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Dynamic model of the haeme-haeme interaction / I. I. Gitel'zon, T. P. Sandalova, Yu. A. Kudenko // Biophysics. - 1973. - Vol. 18, Is. 4. - P804-807 . - ISSN 0006-3509
Аннотация: The possibility of explaining the acceleration of the reaction of oxygenation of haemoglobin from studying the thermodynamic properties of the molecular chains of haemoglobin present in contact with each other is considered. В© 1974.

Scopus
Держатели документа:
Institute of Physics, U.S.S.R. Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitel'zon, I.I.; Sandalova, T.P.; Kudenko, Yu.A.

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Effect of non-central interactions on energy and lattice dynamics of molecular crystals / I. A. Remizov [et al.] // Chemical Physics. - 1985. - Vol. 92, Is. 1. - P163-168 . - ISSN 0301-0104
Аннотация: Atom-atom, multipole-multipole and three-body potentials have been used to calculate the energy and lattice dynamics of ?-paradichlorobenzene, phenanthrene and m-chloronitrobenzene. Non-central interactions are shown to give a significant contribution to the intermolecular interaction energy but have little effect on the phonon spectrum of the crystals. В© 1985.

Scopus
Держатели документа:
L.V. Kirensky Institute of Physics, USSR Academy of Sciences, Siberian Branch, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Remizov, I.A.; Podoprigora, V.G.; Botvich, A.N.; Kharitonova, T.A.; Shabanov, V.F.

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PHOTON-PHONON INTERACTION INFLUENCE ON THE EXTERNAL VIBRATION SPECTRA OF MOLECULAR CRYSTALS. / A. N. Botvich [et al.] // Physica Status Solidi (B) Basic Research. - 1986. - Vol. 134, Is. 2. - P515-522 . - ISSN 0370-1972
Кл.слова (ненормированные):
BENZENE -- PHONONS -- PHOTONS -- EXTERNAL VIBRATION SPECTRA -- M-CHLORONITROBENZENE -- MOLECULAR CRYSTALS -- PHOTON-PHONON INTERACTION -- CRYSTALS
Аннотация: A method is developed to determine the parameters of electromagnetic and elastic excitations in molecular crystals including the polariton resonance region. The dependence of eigenvectors and eigenfrequencies on the wavevector of elementary excitations is calculated numerically for m-chloronitrobenzene crystals. The conditions are found when the photon-phonon resonance effect becomes observable in the Raman scattering (RS) spectra of this crystal.

Scopus
Держатели документа:
Acad of Sciences of the USSR, Krasnoyarsk, USSR, Acad of Sciences of the USSR, Krasnoyarsk, USSR : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Botvich, A.N.; Shabanov, V.F.; Vtyurin, A.N.; Pozdnyakova, T.A.

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SURFACE PHONONS IN MOLECULAR CRYSTALS. / V. G. Podoprigora [et al.] // Physica Status Solidi (B) Basic Research. - 1987. - Vol. 139, Is. 1. - P109-115 . - ISSN 0370-1972
Кл.слова (ненормированные):
HYDROCARBONS - Surfaces -- PHONONS -- MOLECULAR CRYSTALS -- SURFACE PHONONS -- CRYSTALS
Аннотация: The lattice dynamics of the surface layers of anthracene and beta -paradichlorobenzene crystals as well as of the adsorbed monolayer of paradibromobenzene on beta -paradichlorobenzene is investigated using the method of atom-atom potentials in slab dynamical calculations. The dynamic, structural, and electro-optical parameters of the surface crystal layers are compared with those for the bulk.

Scopus
Держатели документа:
Acad of Sciences of the USSR, Krasnoyarsk, USSR, Acad of Sciences of the USSR, Krasnoyarsk, USSR : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Podoprigora, V.G.; Remizov, I.A.; Shabanov, V.F.; Botvich, A.N.

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EFFECT OF METABOLIC BOTTLENECK ORGANIZATION ON KINETICS OF ENZYMIC SUBSTRATE CONVERSION [Text] / R. P. TRENKENSHU // Mol. Biol. - 1988. - Vol. 22, Is. 6. - P. 1170-1177. - Cited References: 27 . - ISSN 0026-8933

РУБ Biochemistry & Molecular Biology

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
TRENKENSHU, R.P.

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Obtaining bacterial luciferase for bioluminescent analysis / V. S. Bondar [и др.] // Prikladnaya Biokhimiya i Mikrobiologiya. - 1988. - Vol. 24, Is. 6. - С. 745-753 . - ISSN 0555-1099
Кл.слова (ненормированные):
luciferase -- enzyme isolation -- enzyme subunit structure -- molecular weight -- nonhuman -- photobacterium leiognathi

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of the USSR Academy of Sciences, Krasnoyarsk, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bondar, V.S.; Vysotsky, E.S.; Zavoruev, V.V.; Mezhevikin, V.V.; Raibekas, A.A.

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Nucleotide sequence of part of Photobacterium leiognathi lux region / B. A. Illarionov [et al.] // Nucleic Acids Research. - 1988. - Vol. 16, Is. 20. - P9855, DOI 10.1093/nar/16.20.9855 . - ISSN 0305-1048
Кл.слова (ненормированные):
bacterial protein -- luciferase -- article -- bacterial gene -- genetics -- molecular genetics -- nucleotide sequence -- Photobacterium -- Bacterial Proteins -- Base Sequence -- Genes, Bacterial -- Luciferase -- Molecular Sequence Data -- Photobacterium

Scopus
Держатели документа:
Krasnoyarsk State University, Krasnoyarsk, Russian Federation
Institute of Biophysics, Krasnoyarsk, Russian Federation
Institute of Clinical and Experimental Medicine, Novosibirsk, Russian Federation
Novosibirsk Institute of Bioorganic Chemistry, 630090, Novosibirsk, Lavrentjev prospect 8, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Illarionov, B.A.; Protopopova, M.V.; Karginov, V.A.; Mertvetsov, N.P.; Gitelson, J.I.

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Electrooptic parameters of molecular crystals: Technique of calculations / A. N. Botvich [et al.] // CONFERENCE ON LASERS AND ELECTRO-0PTICS. - 1989. - Summaries of Papers Presented at the Conference on Lasers and Electro-Optics (24 April 1989 through 28 April 1989, Baltimore, MD, USA) Conference code: 12771. - P210
Кл.слова (ненормированные):
Benzene -- Computer Simulation -- Electrooptical Effects -- Digest of Paper -- Intermolecular Distances -- Molecular Polarizability -- Molecular Crystals
Аннотация: Computer simulations of electrooptic interactions in solid molecular systems have been widely used with good effect. In these calculations molecules are usually considered point dipoles (molecule-point approximation), their parameters are taken from free molecules, and summations over the crystal lattice (lattice sums) are done by the Ewald method. Synthesis of effective new systems for electrooptic applications results in large complicated molecules much longer than the intermolecular distances in crystals. To take molecular fragmentation directly into account in this approach requires very long computing time. To simplify this problem, the molecular lattice sums are modified by dividing the molecule into fragments and calculating the lattice sums for each fragment. The results are then averaged over the weight fragment polarizabilities. This weighting coefficient is introduced to take account of the anisotropy of the molecular polarizability distribution over the molecular frame. The rest of the calculations are performed in the usual way. The method has been used to calculate linear and nonlinear optic parameters for some substituted benzene crystals with good results.

Scopus
Держатели документа:
L.V. Kirensky Inst of Phys, Krasnoyarsk, USSR : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Botvich, A.N.; Podoprigora, V.G.; Shabanov, V.F.; Vtyurin, A.N.

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   Е041
   М 54


    Методы молекулярной генетики и генной инженерии [Текст] : научное издание / А. В. Мазин, К. Д. Кузнеделов, А. С. Краев и др.; Отв. ред. Р. И. Салганик ; АН СССР. Сиб. отд-ние. Ин-т цитологии и генетики. - Новосибирск : Наука. Сиб. отд-ние, 1990. - 247 с. - Библиогр. в конце глав. - 3450 экз. - ISBN 5-02-029565-5 : 3.10 р.
Авт. указаны на обороте тит. л.

ГРНТИ	34.15.27	34.15.23	34.23.19
УДК	577.21.08

ББК Е041.10в7 + Е041.15в7
Рубрики:
Молекулярная генетика
Генная инженерия
Аннотация: Выделение ДНК... 7 Эндонуклеазы рестрикции II типа: свойства иприменение... 14 Введение метки в ДНК... 25 Блот-гибридизация ДНК накапроновых мембранах... 35 Трансформация E. coli плазмидной ДНК... 39Коннекторный способ клонирования кДНК... 44 Получение геномныхбиблиотек в -векторах... 56 Детекция рекомбинантной ДНК методоммолекулярной гибридизации... 74 Белоксинтезирующие системы in vitro иin vivo... 80 Направленный мутагенез in vitro. Индукция транзиций GC AT... 91 Метод килосеквенирования... 99 Определение нуклеотиднойпоследовательности ДНК методом Максама-Гилберта... 107 Клонирование исеквенирование в М13... 115 Анализ функциональных сайтов в геномахпро- и эукариот... 154DNA separation... 7 Type II restriction endonuclease:properties and application... 14 DNA labelling... 25 DNA blothybridization on kapron membranes... 35 E. coli plasmid DNAtransformation... 39 Connection method of cDNA cloning... 44Producing genomic libraries in lambda-vectors... 56 Recombinant DNAdetection by molecular hybridization technique... 74 Proteinsynthesizing systems in vitro and in vivo... 80 Directed mutagenesisin vitro GC AT transition induction... 91 Kilosequencing methods...99 Detection of DNA nucleotide sequence by Maxam and Gilbertmethod... 107 Cloning and sequencing in M13... 115 Analysis offunctional sites in genomes of pro- and eucells... 154
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Мазин, Александр Владимирович; Кузнеделов, К. Д.; Краев, А. С.; Холодилов, Н. Г.; АН СССР. Сибирское отделение; Институт цитологии и генетики (Новосибирск)
Экземпляры всего: 1
КФ (1)
Свободны: КФ (1)
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10.

Isolation of bioluminescent functions from Photobacterium leiognathi: analysis of luxA, luxB, luxG and neighboring genes / B. A. Illarrionov [et al.] // Gene. - 1990. - Vol. 86, Is. 1. - P89-94 . - ISSN 0378-1119
Кл.слова (ненормированные):
Bioluminescence -- expression in E. coli -- luciferase -- molecular evolution -- nucleotide sequence -- protein alignment -- recombinant DNA -- luciferase -- amino acid sequence -- article -- bioluminescence -- fungus -- gene structure -- genetic engineering -- heredity -- nonhuman -- nucleotide sequence -- priority journal -- vibrionaceae -- Acyltransferases -- Amino Acid Sequence -- Bacterial Proteins -- Base Sequence -- Cloning, Molecular -- DNA, Bacterial -- Genes, Structural, Bacterial -- Luciferase -- Luminescence -- Molecular Sequence Data -- Operon -- Photobacterium -- Restriction Mapping -- Escherichia coli -- Fungi -- Photobacterium leiognathi -- Vibrio harveyi -- Vibrionaceae
Аннотация: Genes encoding luminescence of Photobacterium leiognathi have been cloned in Escherichia coli. The luminescent clones were readily apparent. Among them, a clone containing a recombinant plasmid with a 13.5-kb insertion was identified. This DNA fragment contained all of the luminescence-encoding genes. The luciferase-encoding genes (lux) in this DNA fragment were localized. We have sequenced a part of the cloned lux region and identified the luxA, luxB and luxG genes encoding the ? and ? subunits of luciferase and a ? protein with an Mr of 26 180, respectively. The analysis of deduced amino acid sequences and comparison with known luciferase sequences from Vibrio harveyi, indicate the common origin of these proteins. В© 1990.

Scopus
Держатели документа:
Krasnoyarsk State University, Krasnoyarsk, 660062, Russian Federation
All-Union Research Institute of Molecular Biology, Novosibirsk Region, 633159, Russian Federation
Institute of Biophysics, Krasnoyarsk, 660036, Russian Federation
Institute of Clinical and Experimental Medicine, Novosibirsk, Russian Federation
Novosibirsk Institute of Bioorganic Chemistry, Novosibirsk, 630090, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Illarrionov, B.A.; Blinov, V.M.; Douchenko, A.P.; Protopopova, M.V.; Karginov, V.A.; Mertvetsov, N.P.; Gitelson, J.I.

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11.

ISOLATION AND PROPERTIES OF VARIOUS MOLECULAR-FORMS OF CA2+-ACTIVATED PHOTOPROTEIN OBELIN [Текст] / Y. S. VYSOTSKII, V. S. BONDAR, I. I. GITELZON // DOKLADY AKADEMII NAUK SSSR. - 1991. - Vol. 321, Is. 1. - С. 214-217. - Cited References: 14 . - ISSN 0002-3264

РУБ Multidisciplinary Sciences
Рубрики:
CALCIUM-ACTIVATED PHOTOPROTEINS
   CTENOPHORES MNEMIOPSIS SP
   BEROE-OVATA
   AEQUORIN
   PROTEIN
   PURIFICATION
   EXTRACTION
   PHIALIDIN
   SEQUENCE
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
VYSOTSKII, Y.S.; BONDAR, V.S.; GITELZON, I.I.

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12.

Green flavoprotein from P. leiognathi: purification, characterization and identification as the product of the lux G(N) gene / A. A. Raibekas // Journal of bioluminescence and chemiluminescence. - 1991. - Vol. 6, Is. 3. - P. 169-176 . - ISSN 0884-3996
Кл.слова (ненормированные):
bacterial protein -- flavoprotein -- amino acid sequence -- article -- bacterial gene -- chemistry -- genetics -- isolation and purification -- luminescence -- molecular genetics -- molecular weight -- Photobacterium -- Amino Acid Sequence -- Bacterial Proteins -- Flavoproteins -- Genes, Bacterial -- Luminescence -- Molecular Sequence Data -- Molecular Weight -- Photobacterium -- Support, U.S. Gov't, P.H.S.
Аннотация: A green flavoprotein (GFP) was isolated and purified to homogeneity from Photobacterium leiognathi, strain 208. GFP is a homodimer of molecular weight 54,000 and contains two molecules of an unusual flavin per molecule of protein. Various biochemical characteristics including isoelectric point, trypsin and chymotrypsin degradation, SDS and temperature influence on subunit dissociation and the dissociation of the flavin chromophore, were investigated. The sequence of 23 N-terminal amino acids was determined and found to be concurrent with the N-terminal amino acid sequence encoded by the lux G(N) gene of P. leiognathi. This fact suggests that GFP is a structural component of the Photobacterium luminescence system.

Scopus
Держатели документа:
Institute of Biophysics, USSR Academy of Sciences, Krasnoyarsk. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Raibekas, A.A.

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13.

PHYSICOCHEMICAL PROPERTIES OF A PHOTOPROTEIN FROM THE HYDROID POLYP OBELIA-LONGISSIMA [Text] / V. S. BONDAR, K. P. TROFIMOV, E. S. VYSOTSKII // Biochem.-Moscow. - 1992. - Vol. 57, Is. 10. - P1020-1027. - Cited References: 36 . - 8. - ISSN 0006-2979

РУБ Biochemistry & Molecular Biology
Рубрики:
CALCIUM-ACTIVATED PHOTOPROTEINS
   CTENOPHORES MNEMIOPSIS SP
   BEROE-OVATA
   AEQUORIN
   CA-2+
   INDICATORS
   PROTEIN
   BINDING
   PURIFICATION
   EXTRACTION
Кл.слова (ненормированные):
BIOLUMINESCENCE -- CA2+-ACTIVATED PHOTOPROTEIN -- OBELIN -- CHROMATOGRAPHY -- CALCIUM
Аннотация: The photoprotein obelin was isolated and purified to homogeneity (as indicated by sodium dodecyl sulfate polyacrylamide gel electrophoresis) from hydroids of Obelia longissima by gel filtration on Sephadex G-75 fine, ion exchange chromatography on Polysil CA-300 (10 mum), hydrophobic chromatography on Phenyl-Sepharose CL-4B, gel filtration on Sephacryl S-200 superfine, ion exchange chromatography on a Mono Q column at pH 7.0, chromatofocusing on a Mono P column (pH gradient 6.0-4.0), and ion exchange chromatography on a Mono Q column at pH 5.5, 8.8, and 7.0. The molecular weight of the native protein was 30 kD, and that measured in the presence of SDS was 19.8 kD. The specific activity of obelin is 4.9.10(15) quanta/mg protein, pseudo-first-order constant of bioluminescence decay 4 sec-1, and quantum yield 0.16 The range of measurable Ca2+ concentrations is 10(-7) to 10(-5) M. The luminescence spectrum of obelin peaks at 469 nm, and the fluorescence emission maximum of the discharged protein is at 455 nm. The optimum pH for luminescence is between 9.0 and 10.5. The molecular ionization constants are pK1 6.8 and pK2 12.2, and the ionization constants for the active site are pK1 9.1 and pK2 10.2
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
BONDAR, V.S.; TROFIMOV, K.P.; VYSOTSKII, E.S.

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14.

The comparative redundancy of genes of various organisms and viruses / A. N. Gorban [и др.] // Genetika. - 1993. - Vol. 29, Is. 9. - P. 1413-1419 . - ISSN 0016-6758
Кл.слова (ненормированные):
article -- dna content -- evolution -- genome -- nonhuman -- restriction mapping -- virus -- amino acid sequence -- comparative study -- gene frequency -- human -- molecular genetics -- nucleotide sequence -- restriction mapping -- virus gene -- Amino Acid Sequence -- Base Sequence -- Comparative Study -- English Abstract -- Gene Frequency -- Genes, Viral -- Human -- Molecular Sequence Data -- Restriction Mapping

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gorban, A.N.; Mirkes, E.M.; Popova, T.G.; Sadovsky, M.G.

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15.

REDUNDANCY OF GENETIC TEXTS [Text] / A. N. GORBAN, T. G. POPOVA, M. G. SADOVSKII // Mol. Biol. - 1994. - Vol. 28, Is. 2. - P. 206-213. - Cited References: 14 . - ISSN 0026-8933

РУБ Biochemistry & Molecular Biology

Кл.слова (ненормированные):
GENE -- CORRELATION -- REDUNDANCY
Аннотация: A new method is proposed for measuring and comparing the redundancy of genetic texts. It is based on compiling for a given text a frequency/correlation dictionary encompassing all the words (subsequences) of length from 1 to N encountered in the text (N is text length); the measure of redundancy is the minimal length at which all words are unique. Preliminary results are reported for different genetic texts.

WOS
Держатели документа:
RUSSIAN ACAD SCI,INST BIOPHYS,KRASNOYARSK 660036,RUSSIA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
GORBAN, A.N.; POPOVA, T.G.; SADOVSKII, M.G.

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16.

MOLECULAR-STRUCTURE AND PHYSICOCHEMICAL PROPERTIES STUDY OF POLYHYDROXYBUTYRATE [Текст] / O. V. FALALEEV [и др.] // Dokl. Akad. Nauk. - 1994. - Vol. 337, Is. 6. - С. 813-817. - Cited References: 13 . - 5. - ISSN 0869-5652

РУБ Multidisciplinary Sciences
Рубрики:
POLY(BETA-HYDROXYBUTYRATE)
BIOTECHNOLOGY

Держатели документа:
LV KIRENSKII INST PHYS,KRASNOYARSK,RUSSIA : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
FALALEEV, O.V.; VOLOVA, T.G.; ZEER, E.P.; VASILEV, A.D.

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17.

SEQUENCE OF THE CDNA-ENCODING THE CA2+-ACTIVATED PHOTOPROTEIN OBELIN FROM THE HYDROID POLYP OBELIA-LONGISSIMA [Text] / B. A. ILLARIONOV [et al.] // Gene. - 1995. - Vol. 153, Is. 2. - P273-274, DOI 10.1016/0378-1119(94)00797-V. - Cited References: 6 . - 2. - ISSN 0378-1119

РУБ Genetics & Heredity
Рубрики:
CA-2+-ACTIVATED PHOTOPROTEIN
AEQUORIN
CLONING
Кл.слова (ненормированные):
BIOLUMINESCENCE -- CALCIUM -- GENE -- PLASMID -- MARINE COELENTERATES
Аннотация: A cDNA clone encoding the Ca2+-activated photoprotein, obelin (Obl), from Obelia longissima was sequenced. The nucleotide (nt) sequence contained two long overlapping open reading frames (ORFs), one of which encoded apoobelin (apoObl). The deduced amino acid (aa) sequence of apoObl revealed that this 195-aa protein has three EF-hand structures that are characteristic for Ca2+-binding domains. Strong aa homology was shown among apoObl, apoaequorin and apoclytin. The second ORF present in the obl cDNA consists of 139 codons and encodes a very basic protein with a calculated pI of 10.56 and a molecular mass of 16153 Da.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
ILLARIONOV, B.A.; BONDAR, V.S.; ILLARIONOVA, V.A.; VYSOTSKI, E.S.

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18.

OBELIN MESSENGER-RNA - A NEW TOOL FOR STUDIES OF TRANSLATION IN CELL-FREE SYSTEMS [Text] / S. V. MATVEEV [et al.] // Anal. Biochem. - 1995. - Vol. 231, Is. 1. - P34-39, DOI 10.1006/abio.1995.1499. - Cited References: 17 . - ISSN 0003-2697

РУБ Biochemical Research Methods + Biochemistry & Molecular Biology + Chemistry, Analytical
Рубрики:
MESSENGER-RNA
   AEQUORIN
   PROTEIN
   CLONING
   CDNA
Аннотация: Obelin mRNA obtained in vitro with the aid of SP6 RNA polymerase was translated in a wheat germ cell-free system, Only the polypeptide with a molecular mass of about 20 kDa was synthesized. The activation of apoobelin with a synthetic coelenterazine revealed a luminescence activity initiated by calcium. The specific activity was 3.6 +/- 0.4 x 10(15) photons per mg of the in vitro synthesized obelin (k = 6.9 s(-1)). The luminescence of the obelin was in a good correlation with the protein concentration calculated by the incorporation of [C-14]Leu. The determination of the amount of de novo synthesized obelin based on measurement of its luminescence is one-thousand times more sensitive than the approach based on the incorporation of labeled amino acid. Thus, obelin mRNA has some advantages for evaluating the efficiency of cell-free translation when compared with standard methods. (C) 1995 Academic Press, Inc.

Держатели документа:
RUSSIAN ACAD SCI,INST BIOPHYS,KRASNOYARSK 660036,RUSSIA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
MATVEEV, S.V.; ILLARIONOV, B.A.; VYSOTSKI, E.S.; BONDAR, V.S.; MARKOVA, S.V.; ALAKHOV, Y.B.

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19.

Properties of recombinant fluorescent proteins from Photobacterium leiognathi and their interaction with luciferase intermediates / V. N. Petushkov, B. G. Gibson, J. Lee // Biochemistry. - 1995. - Vol. 34, Is. 10. - P3300-3309 . - ISSN 0006-2960
Кл.слова (ненормированные):
luciferase -- recombinant protein -- article -- ligand binding -- nonhuman -- priority journal -- protein isolation -- protein protein interaction -- protein stability -- vibrionaceae -- Bacterial Proteins -- Binding Sites -- Carrier Proteins -- Circular Dichroism -- Flavin Mononucleotide -- Fluorescence Polarization -- Genes, Bacterial -- Kinetics -- Ligands -- Luciferase -- Luminescence -- Molecular Sequence Data -- Photobacterium -- Recombinant Proteins -- Spectrophotometry -- Support, U.S. Gov't, P.H.S. -- Photobacterium leiognathi -- Vibrionaceae
Аннотация: Ligand binding and luciferase interaction properties of the recombinant protein corresponding to the lumazine protein gene (EMBL X56534) of Photobacterium leiognathi have been determined by fluorescence dynamics, circular dichroism, gel filtration, and SDS-PAGE. Scatchard analysis of a fluorescence titration shows that the apoprotein possess one binding site, and at 30В°C the KdS (?M) are as follows: 6,7-dimethyl-8-ribityllumazine, 0.26; riboflavin, 0.53; and much more weakly bound FMN, 30. All holoproteins are highly fluorescent and have absorption spectra distinct from each other and from the free ligands. The longest wavelength absorption maxima are, respectively (nm, 2В°C), 420,463, and 458. Ligand binding produces no change in the far-UV circular dichroism; all have mean residual ellipticity at 210 nm of -6500 deg cm2 dmol-1, the same as the native protein. However, in the bioluminescence reaction only the lumazine holoprotein shows a bioluminescence effect. Fluorescence emission anisotropy decay was used to establish that none of these holoproteins complexed with native luciferase and that the lumazine protein alone formed a 1:1 complex with the luciferase hydroxyflavin fluorescent transient and the luciferase peroxyflavin intermediates, revealed by a dominant channel of anisotropy loss, with rotational correlation time of 2.5 ns, and attributed to excitation transfer from the luciferase flavin donor to the acceptor, the lumazine ligand. The complex stability was sufficient to allow its isolation by FPLC gel filtration and verification by SDS-PAGE. These methods also confirmed the absence of interaction of the holoflavoproteins.

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Держатели документа:
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, United States
Institute of Biophysics, Academy of Sciences of Russia (Siberian Branch), 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Petushkov, V.N.; Gibson, B.G.; Lee, J.

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20.

REDUNDANCY OF EUKARYOTIC DNA DECREASES AFTER EXCISION OF INTRONS [Text] / T. G. POPOVA, M. G. SADOVSKII // Mol. Biol. - 1995. - Vol. 29, Is. 3. - P. 281-285. - Cited References: 23 . - ISSN 0026-8933

РУБ Biochemistry & Molecular Biology
Рубрики:
SEQUENCES
Кл.слова (ненормированные):
EXON -- INTRON -- WORD -- FREQUENCY -- REDUNDANCY
Аннотация: The article is devoted to analysis of the internal gene structure with respect to redundancy of exons and introns. Human genes with precisely determined exon-intron structure were studied. Redundancy of each exon and intron within a certain gene was measured. In the analyzed human genes, introns were more redundant than exons. Redundancy was determined as the minimal length of a word (oligonucleotide) that is present as a single copy within the nucleotide sequence studied. Mechanisms are discussed that lead to disturbances in the relationships found between the redundancies of exons and introns.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
POPOVA, T.G.; SADOVSKII, M.G.

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