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   Е071
   Б 63

    Шевырногов, Анатолий Петрович.
    Биосфера - взгляд сверху (экспрессные методы мониторинга биосферы в ИБФ СО РАН – ХХ–ХХI вв.) [Текст] = biosphere - a view from space (express methods of the biosphere monitoring in the Institute of Biophysics SB RAS – XX–XXI century) / А. П. Шевырногов, Д. В. Дементьев // Биофизика для экологии и медицины: к 90-летию академика РАН И. И. Гительзона / И. И. Гительзон, Т. Г. Волова, А. Г. Дегерменджи [и др.] ; ред., авт. предисл. Т. Г. Волова. - Новосибирск : Издательство Сибирского отделения Российской академии наук, 2019. - С. 180-193. - Библиогр.: с. 193 . - ISBN 978-5-7692-1650-3

УДК

577

574

ББК Е071я43 + Р252.0я43

Доп.точки доступа:
Гительзон, Иосиф Исаевич; Волова, Татьяна Григорьевна; Дегерменджи, Андрей Георгиевич; Дегерменджи, Н. Н.; Кратасюк, В. А.; Барцев, Сергей иванович; Болсуновский, Александр Яковлевич; Бондарь, Владимир Антонович; Буров, А. Е.; Величко, В. В.; Гладышев, Михаил Иванович; Есимбекова, Е. Н.; Дементьев, Д. В.; Задереев, Егор Сергеевич; Зотина, Т. А.; Косиненко, Сергей Васильевич; Медведева, С. Е.; Петушков, В. Н.; Печуркин, Николай Савельевич; Прокопкин, И. Г.; Пузырь, А. П.; Пуртов, К. В.; Рогозин, Денис Юрьевич; Родионова, Н. С.; Ронжин, Н. О.; Сомова, Лидия Александровна; Тихомиров, Александр Аполлинариевич; Тихомирова, Наталья Александровна; Трифонов, С. В.; Ушакова, Софья Аврумовна; Франк, Л. А.; Хромечек, Е. Б.; Шишацкая, Е. И.; Шуваев, А. Н.; Российская академия наук. Сибирское отделение; Институт биофизики(Красноярск)

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   Е071
   Б 63
Е07 / Б 63-ИБФ-КФ


    Биофизика для экологии и медицины: к 90-летию академика РАН И. И. Гительзона [Текст] / И. И. Гительзон, Т. Г. Волова, А. Г. Дегерменджи [и др.] ; ред., авт. предисл. Т. Г. Волова ; Российская академия наук, Сибирское отделение, Институт биофизики (Красноярск). - Новосибирск : Издательство Сибирского отделения Российской академии наук, 2019. - 292, [2] с. : ил., цв. ил. ; 25 см. - Рез. ст. англ. - Библиогр. в конце ст. - 300 экз. - ISBN 978-5-7692-1650-3 : 1635.00 р.
    Содержание:
Гительзон, Иосиф Исаевич. Краткий очерк истории, состояния и перспектив = A short essay on the history, state and prospects of the institute of biophysics FRC KSC SB RAS / И. И. Гительзон. - С .14-23
Медведева, С. Е. Коллекция культур ибсо как база для исследований биолюминесценции й и грибов в ИБФ СО РАН = Culture collection ibso as a basis for research of bioluminescence of bacteria and fungi in IBP SB RAS / С. Е. Медведева. - С .24-39. - Библиогр.: с. 37-39
Гительзон, Иосиф Исаевич. Биолюминесценция Мирового океана = Bioluminescence of the World Ocean / И. И. Гительзон, Л. А. Левин, А. С. Артемкин, Р. Н., Чепилов В. В., Молвинских С.Л., Черепанов О. А., Чугунов Ю. В., Караев Н. Д., Загородний Ю. А., Шевырногов А. П. Утюшев Р. Н. - С .40-60. - Библиогр.: с. 60
Другие авторы: Левин Л. А., Артемкин А. С., Утюшев Р. Н., Чепилов В. В., Молвинских С.Л., Черепанов О. А., Чугунов Ю. В., Караев Н. Д., Загородний Ю. А., Шевырногов А. П.
Кратасюк, В. А. Бактериальная люцифераза в биолюминесцентном анализе = Bacterial luciferase in bioluminescent analysis / В. А. Кратасюк, Е. Н. Есимбекова. - С .61-71. - Библиогр.: с. 70-71
Франк, Л. А. Целентеразин-зависимые биолюминесцентные системы = Coelenterazine-dependent bioluminescent systems / Л. А. Франк. - С .72-87. - Библиогр.: с. 85-87
Кл.слова: люцифераза
Пуртов, К. В. Изучение химического механизма биолюминесценции грибов = The study of the chemical mechanism of bioluminescence of fungi / К. В. Пуртов, В. Н. Петушков, Н. С. Родионова. - С .88-98. - Библиогр.: с. 98
Родионова, Н. С. Исследование биолюминесценции сибирских почвенных олигохет = Study of siberian bioluminescent earthworms / Н. С. Родионова, А. А. Петушков. - С .99-118. - Библиогр.: с. 116-118
Тихомиров, А. А. Экспериментальные модели замкнутых экосистем с расчетной долей человека как перспективное направление исследований по созданию биолого-технической системы жизнеобеспечения = Experimental models of closed ecosystems with the human calculated limits as a perspective direction of research on the creation of BTLSS / А. А. Тихомиров, С. А. Ушакова, Н. А. Тихомирова, С. В., Величко В. В. Трифонов С. В. - С .119-128. - Библиогр.: с. 128
Другие авторы: Ушакова С. А., Тихомирова Н. А., Трифонов С. В., Величко В. В.
Волова, Татьяна Григорьевна. Управляемый биосинтез: от параметрически управляемых продуцирующих биосистем до новейших биофизических технологий = Controlled biosynthesis: from parametrically controlled producing biosystems to newest biophysical technologies / Т. Г. Волова, Е. И. Шишацкая. - С .129-148. - Библиогр.: с. 147-148
Бондарь, Владимир Станиславович. Биомедицинские приложения наноалмазов взрывного синтеза = Biomedical applications of nanodiamonds of explosive synthesis / В. С. Бондарь, А. П. Пузырь, Н. О. Ронжин, А. В., Буров А. Е. Барон А. В. - С .149-165. - Библиогр.: с. 161-165
Другие авторы: Пузырь А. П., Ронжин Н. О., Барон А. В., Буров А. Е.
Болсуновский, Александр Яковлевич. Применение радиоизотопных методов в институте биофизики СО РАН: от клеток крови до экосистем = Use od radioisotope techniques in the Institute of Biophysics SB RAS: from blood cells to ecosystems / А. Я. Болсуновский, С. В. Косиненко, Т. А. Зотина, Д. В. Дементьев. - С .166-179. - Библиогр.: с. 177-179
Другие авторы: Косиненко С. В., Зотина Т. А., Дементьев Д. В.
Шевырногов, Анатолий Петрович. Биосфера - взгляд сверху (экспрессные методы мониторинга биосферы в ИБФ СО РАН – ХХ–ХХI вв.) = biosphere - a view from space (express methods of the biosphere monitoring in the Institute of Biophysics SB RAS – XX–XXI century) / А. П. Шевырногов. - С .180-193. - Библиогр.: с. 193
Гладышев, Михаил Иванович. Жирные кислоты в экологической биофизике водных систем = Fatty acids in ecological biophysics of aquatic ecosystems / М. И. Гладышев. - С .194-209. - Библиогр.: с. 206-209
Рогозин, Денис Юрьевич. Сравнительное исследование устойчивости стратификации и структуры трофической сети в меромиктических озерах Шира и Шунет (Южная Сибирь, Россия) = Comparative study of the stability of stratification and the food web structure in the meromictic lakes Shira and Shunet (South Siberia, Russia) / Д. Ю. Рогозин, Е. С. Задереев, И. Г. Прокопкин [и др.]. - С .210-247. - Библиогр.: с. 243-247
Другие авторы: Задереев Е. С., Прокопкин И. Г., Толомеев А. П., Бархатов Ю. В., Хромечек Е. Б., Дегерменджи Н. Н., Дроботов А. В., Дегерменджи А. Г.
Печуркин, Николай Савельевич. Непрерывный рост интенсивности энерго-вещественных взаимодействий в эволюции геобиосферы Земли = Transparent growth of the energy/matter interactions on Earth in the evolution of geobiosphere / Н. С. Печуркин, А. Н. Шуваев, Л. А. Сомова. - С .248-254
Барцев, Сергей Иванович. Малоразмерные модели биосферы и феноменология изменения глобального климата = Small-scale biosphere models and phenomenology of global climate change / С. И. Барцев, А. Г. Дегерменджи. - С .255-283. - Библиогр.: с. 281-283
Дегерменджи, Андрей Георгиевич. Направления развития биофизики в Красноярске / А. Г. Дегерменджи. - С .284-288

ГРНТИ	31.27	76.03
УДК	577	574	61

ББК Е071я43 + Р252.0я43
Рубрики:
Экологическая биофизика
Медицинская биофизика
Кл.слова (ненормированные):
биолюминесценция -- люцифераза -- целентаразин -- олигохеты -- замкнутые экосистемы -- управляемый биосинтез -- наноалмазы -- радиоизотопные методы -- биосфера -- жирные кислоты -- системы жизнеобеспечения -- меромиктические озера -- геобиосфера -- эволюция -- глобальный климат -- Медицинская биофизика
Аннотация: Сборник посвящен широкому кругу исследований в области экологической биофизики – научного направления на стыке наук – от исследований на молекулярном уровне до вопросов управления большими природными экосистемами. Рассмотрены исторические вехи развития экологического направления биофизики. Основной акцент сборника основан на современных, актуальных достижениях красноярских биофизиков, которым удалось сохранить и развить многоплановые направления, которые были заложены в 50-х гг. ХХ века И. И. Гительзоном. Наряду с обзорными материалами и результатами фундаментальных исследований представлен ряд готовых к внедрению биотехнологий. Книга адресована биофизикам, экологам и химикам, а также преподавателям и студентам биофизических, биологических и экологических кафедр университетов.

Держатели документа:
Библиотека Института биофизики СО РАН : 660036, Академгородок, 50/12

Доп.точки доступа:
Гительзон, Иосиф Исаевич; Волова, Татьяна Григорьевна; Дегерменджи, Андрей Георгиевич; Дегерменджи, Н. Н.; Шевырногов, Анатолий Петрович; Кратасюк, В. А.; Барцев, Сергей иванович; Болсуновский, Александр Яковлевич; Бондарь, Владимир Антонович; Буров, А. Е.; Величко, В. В.; Гладышев, Михаил Иванович; Есимбекова, Е. Н.; Дементьев, Д. В.; Задереев, Егор Сергеевич; Зотина, Т. А.; Косиненко, Сергей Васильевич; Медведева, С. Е.; Петушков, В. Н.; Печуркин, Николай Савельевич; Прокопкин, И. Г.; Пузырь, А. П.; Пуртов, К. В.; Рогозин, Денис Юрьевич; Родионова, Н. С.; Ронжин, Н. О.; Сомова, Лидия Александровна; Тихомиров, Александр Аполлинариевич; Тихомирова, Наталья Александровна; Трифонов, С. В.; Ушакова, Софья Аврумовна; Франк, Л. А.; Хромечек, Е. Б.; Шишацкая, Е. И.; Шуваев, А. Н.; Волова, Татьяна Григорьевна \ред., авт. предисл.\; Утюшев Р. Н., Чепилов В. В., Молвинских С.Л., Черепанов О. А., Чугунов Ю. В., Караев Н. Д., Загородний Ю. А., Шевырногов А. П.; Трифонов С. В., Величко В. В.; Барон А. В., Буров А. Е.; Толомеев А. П., Бархатов Ю. В., Хромечек Е. Б., Дегерменджи Н. Н.; Дроботов А. В.; Дегерменджи А. Г., Андрей Георгиевич; Гительзон, Иосиф Исаевич \о нем\; Российская академия наук. Сибирское отделение; Институт биофизики (Красноярск)
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Variability of fluorescence spectra of coelenteramide-containing proteins as a basis for toxicity monitoring / R. R. Alieva, N. S. Kudryasheva // Talanta. - 2017. - Vol. 170. - P425-431, DOI 10.1016/j.talanta.2017.04.043 . - ISSN 0039-9140
Кл.слова (ненормированные):
Coelenteramide-containing fluorescent protein -- Multicolor fluorescent bioassay -- Obelin -- Primary photochemical process -- Protein destruction -- Proton transfer -- Bioassay -- Biomarkers -- Excited states -- Fluorescence -- Fluorophores -- Ionizing radiation -- Proton transfer -- Toxicity -- Electron-excited state -- Fluorescence spectra -- Fluorescent protein -- Green fluorescent protein -- Obelin -- Photochemical process -- Photochemical properties -- Physicochemical process -- Proteins
Аннотация: Nowadays, physicochemical approach to understanding toxic effects remains underdeveloped. A proper development of such mode would be concerned with simplest bioassay systems. Coelenteramide-Containing Fluorescent Proteins (CLM-CFPs) can serve as proper tools for study primary physicochemical processes in organisms under external exposures. CLM-CFPs are products of bioluminescent reactions of marine coelenterates. As opposed to Green Fluorescent Proteins, the CLM-CFPs are not widely applied in biomedical research, and their potential as colored biomarkers is undervalued now. Coelenteramide, fluorophore of CLM-CFPs, is a photochemically active molecule; it acts as a proton donor in its electron-excited states, generating several forms of different fluorescent state energy and, hence, different fluorescence color, from violet to green. Contributions of the forms to the visible fluorescence depend on the coelenteramide microenvironment in proteins. Hence, CLM-CFPs can serve as fluorescence biomarkers with color differentiation to monitor results of destructive biomolecule exposures. The paper reviews experimental and theoretical studies of spectral-luminescent and photochemical properties of CLM-CFPs, as well as their variation under different exposures – chemicals, temperature, and ionizing radiation. Application of CLM-CFPs as toxicity bioassays of a new type is justified. © 2017

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Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Akademgorodok 50/50, Krasnoyarsk, Russian Federation
Siberian Federal University, Svobodny Prospect 79, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Alieva, R. R.; Kudryasheva, N. S.

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Using DCMU-fluorescence method for the identification of dominant phytoplankton groups [Text] / N. A. Gaevsky [et al.] // J. Appl. Phycol. - 2005. - Vol. 17, Is. 6. - P. 483-494, DOI 10.1007/s10811-005-2903-x. - Cited References: 28 . - ISSN 0921-8971

РУБ Biotechnology & Applied Microbiology + Marine & Freshwater Biology
Рубрики:
IN-VIVO
   CHLOROPHYLL FLUORESCENCE
   FLUOROMETRIC METHOD
   SITU
   PHOTOSYNTHESIS
   CYANOBACTERIA
   ALGAE
   EVOLUTION
   CULTURES
   LAKES
Кл.слова (ненормированные):
algae -- chlorophyll -- fluorescence -- vizualization
Аннотация: For the identification of ecologically significant dominant groups of phytoplanktonic algae a polychromatic DCMU-induced fluorescence method is recommended. A special fluorometer equipped with a system of replaceable filters is used to differentiate three regions of the spectrum (410 +/- 20, 510 +/- 20 and 540 +/- 10 nm) that can excite the basic light-harvesting pigments. Total and differential (for every algal taxon studied) chlorophyll a calculated from the fluorescence signals is in good agreement with biomass estimates from direct cell counts for several different trophic types of aquatic systems. This is made possible by the vizualization of the ratios of fluorescence signal values in their own coordinates: first, to decide whether it is necessary to correct linear equations in order to eliminate negative solutions; second, to determine the possibility of nulling the negative solution if a point is situated close to a side of the triangle; and third, to reduce the number of linear algebraic equations to two if the points are situated along one of the triangle sides or to one if the points are gathered at the apex. The polychromatic DCMU-induced fluorescence method can be used for monitoring natural phytoplankton populations to detect changes in their taxonomic structure.

WOS
Держатели документа:
Krasnoyarsk State Univ, Dept Biol, Krasnoyarsk, Russia
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gaevsky, N.A.; Kolmakov, V.I.; Anishchenko, O.V.; Gorbaneva, T.B.

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Use of unmanned aerial vehicles for sensing microrelief during agrocenoses monitoring / A. P. Shevyrnogov, N. O. Malchikov, I. Y. Botvich [et al.] // CEUR Workshop Proceedings : CEUR-WS, 2019. - Vol. 2534: 2019 All-Russian Conference ""Spatial Data Processing for Monitoring of Natural and Anthropogenic Processes"", SDM 2019 (26 August 2019 through 30 August 2019, ) Conference code: 156641. - P485-490
Кл.слова (ненормированные):
Agriculture -- Barley -- Precision farming -- Spectrometry -- UAV -- Vegetation index -- Agriculture -- Antennas -- Data handling -- Spectrometry -- Unmanned aerial vehicles (UAV) -- Vegetation -- Agricultural land -- Barley -- Ground based -- Integral values -- Microrelief -- Precision farming -- Vegetation index -- Vegetation periods -- Monitoring
Аннотация: The use of UAVs for obtaining data on field microrelief within agricultural lands was demonstrated. The work relies on the NDVI integral values during the vegetation period obtained using satellite and ground-based spectrometry. It was established that a change in microrelief has a significant impact on the value of the NDVI integral and yield. The proposed approach can be used in precision farming for planning future agricultural work. Copyright © 2019 for this paper by its authors. Use permitted under Creative Commons License Attribution 4.0 International (CC BY 4.0).

Scopus
Держатели документа:
Institute of Biophysics SB RAS, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Shevyrnogov, A. P.; Malchikov, N. O.; Botvich, I. Y.; Yemelianov, D. V.; Larko, A. A.

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Understanding the mechanisms of blooming of phytoplankton in Lake Shira, a saline lake in Siberia (the Republic of Khakasia) / A. G. Degermendzhy, R. D. Gulati // Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - P331-340 . - ISSN 1386-2588
Кл.слова (ненормированные):
Carbon budget -- Cyanobacteria -- Heterotrophic bacteria -- Hydrogen sulphide -- Mathematical models of stratification -- Meromictic lakes -- Microbial loop -- Stratification -- Trophic scheme -- algal bloom -- ecosystem modeling -- limiting factor -- nutrient availability -- phytoplankton -- saline lake -- trophic interaction -- Russian Federation -- algae -- Bacteria (microorganisms) -- Cyanobacteria -- Lyngbya -- Lyngbya contorta
Аннотация: The paper summarises the results of a three-year research study (European Union Grant: INTAS 97-0519) aimed at investigating the planktonic populations and trophic organization of the Lake Shira ecosystem - a saline lake in Khakasia, Siberia. The lake exhibits a stable summer-autumn stratification of the chemical-biological components. The mechanisms responsible for the 'blooming' of phytoplankton in the deeper layers were investigated in greater detail, using data from both field and laboratory experiments. The spectra of nutrition were examined to estimate the relationships between the specific growth rates of the hydrobionts and the influence of the limiting factors: light, nutrients. The observed heterotrophic capability of a metalimnetic phytoplankton population might help explain the development in the deeper waters of Lyngbya contorta. The scheme of trophic interactions was put up, based on the assessment of the carbon pools and carbon flows in the pelagic zone of the lake. A mathematical model of the vertical structure of the lake's plankton populations was constructed, using the ecosystem description and data of vertical turbulent diffusion. The role of light and nutrient limitations and grazing mortality in forming the vertical inhomogeneities, particularly in lowering the depth of the maximal cyanobacterial biomass, has been demonstrated. The theoretical curves for the stratification of chemical and biological parameters have been brought in conformity with the field observations, e.g. for the different patterns of the peaks, and for the biomass maxima of cyanobacteria, purple and green sulphur bacteria, oxygen, and hydrogen sulphide. The calculations revealed that for an adequate assessment of the parameters for the hydrogen sulphide zone it is necessary to introduce flows of allochthonous organic matter. Based on the form of the sulphur distribution curve, the allochthonous input of organic matter and the inflow of hydrogen sulphide from the bottom have been theoretically discriminated for the first time. It has also been ascertained that irrespective of the depth the allochthonous substances limiting bacterial growth, the bacteria are uniformly distributed over depth and can serve as an indicator of the presence of limitation (the effect of autostabilisation in space). Of indisputable interest to limnology are the specific methods developed for understanding the functioning of Lake Shira ecosystem. These include the autostabilisation of the limiting factors, the on-the-spot fluorescent method of determining the three classes of microalgae, the algal mixotrophy and the planktonic population interactions and feedbacks, and development of a more sensitive, bioluminescent method for mapping the nonhomogeneities. Owing to a balanced combination of classical approaches (field observations, in situ data on production-decomposition) and the more recent ones (satellite monitoring, biophysical methods of estimating interactions of populations, mathematical models based on the field and experimental data), many of the structural-function relationships in the ecosystem can now be explained, and the models can provide 'mutual control and mutual agreement' between the data collected using different approaches.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Degermendzhy, A.G.; Gulati, R.D.

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Tritium in surface waters of the Yenisei River basin / A. Y. Bolsunovsky, L. G. Bondareva // Journal of Environmental Radioactivity. - 2003. - Vol. 66, Is. 3. - P285-294, DOI 10.1016/S0265-931X(02)00132-7 . - ISSN 0265-931X
Кл.слова (ненормированные):
Deep-well injection of radionuclides -- Nuclear reactor -- Surface and ground waters -- Tritium -- Yenisei River basin -- Cooling water -- Nuclear reactors -- Sediments -- Surface waters -- River basins -- Tritium -- carbon 14 -- surface water -- tritium -- groundwater -- nuclear power plant -- radioactive pollution -- surface water -- tritium -- article -- catchment -- measurement -- radioactive contamination -- river -- sediment -- Environmental Monitoring -- Geologic Sediments -- Mining -- Power Plants -- Reference Values -- Russia -- Tritium -- Water Pollutants, Radioactive -- Russian Federation -- Tritium
Аннотация: This paper reports an investigation of the tritium content in the surface waters of the Yenisei River basin near the Mining and Chemical Combine (MCC). In 2001 the maximum tritium concentration in the Yenisei River did not exceed 4 В± 1 Bq l-1, which is consistent with the data of 1998-99. However, it has been found that there are surface waters containing enhanced tritium as compared with the background values for the Yenisei River. For instance, in the Ploskii Stream and the Shumikha River the maximum tritium concentrations amount to 168 and 81 Bq l-1, respectively. The source of tritium in these surface waters is the last operating reactor at the MCC, which still uses the Yenisei water as coolant. In water and sediment samples of the Bolshaya Tel River (a tributary of the Yenisei River) the tritium content turned out to be at least 10 times higher than the background values for the Yenisei River. The measurements conducted at the RPA RADON (Moscow) revealed not only tritium but also the artificial radionuclide 14C in the Bolshaya Tel samples. The data obtained suggest that the Bolshaya Tel River receives the major part of tritium from sediments rather than from the water catchment area. This allows the conclusion that there is water exchange between the surface waters and the radioactively contaminated underground horizons of the "Severny" testing site. В© 2002 Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsky, A.Y.; Bondareva, L.G.

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Time-space structure and variability of surface temperature frontal zones in the ocean (based on AVHRR satellite data) / A. V. Kartushinsky // Advances in Space Research. - 2000. - Vol. 25, Is. 5. - P1107-1110, DOI 10.1016/S0273-1177(99)00871-6 . - ISSN 0273-1177
Кл.слова (ненормированные):
AVHRR -- frontal feature -- global ocean -- sea surface temperature -- spatiotemporal analysis
Аннотация: Satellite monitoring of large-scale ocean fields allows collection and analyse of information on dynamics processes in various areas of the ocean. Investigation of the World Ocean processes involves the determination of space-time changes of sea surface temperature (SST). Water temperature of the ocean is one of major characteristics of surface water masses. Distribution of water temperature depends not only on regional climate peculiarities but also on formation of high gradient temperature zones. These zones emerge due to interaction of different current structure, circulation and eddies. Some more important characteristics of such zones are space dimensions and time of existence (lifetime). Space-time scales of the above structure have not been investigated in detail. The aim of the paper is to study space-time changes of the surface temperature frontal zones and frontal partitions in the ocean by AVHRR satellite information.

Scopus
Держатели документа:
Institute of Biophysics (Russian Academy of Sciences, Siberian Branch), Academgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kartushinsky, A.V.

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Time-space earth surface gradients for satellite monitoring of ecosystems / A. V. Kartushinsky, N. A. Ogorodov, A. A. Larko // CEUR Workshop Proceedings : CEUR-WS, 2019. - Vol. 2534: 2019 All-Russian Conference ""Spatial Data Processing for Monitoring of Natural and Anthropogenic Processes"", SDM 2019 (26 August 2019 through 30 August 2019, ) Conference code: 156641. - P366-370
Кл.слова (ненормированные):
Average seasonal variability -- Ecotone -- Frontal zones -- Gradient fields -- Regional features -- Satellite data -- Spatial averaging -- Synergistic effect -- Aquatic ecosystems -- Data handling -- Earth (planet) -- Satellites -- Ecotone -- Frontal zones -- Gradient fields -- Regional feature -- Satellite data -- Seasonal variability -- Spatial averaging -- Synergistic effect -- Monitoring
Аннотация: The results of Earth surface gradients characteristics calculate based on satellite data are presents. Dynamics structural features of horizontal gradient fields in aquatic objects and land ecosystems by physical, biological parameters are considered. Problems of the parametrization for environment exchanges evaluations with using numerical modeling based on satellite data and software tools are submitted. Spatial-temporal scaling and averaging of gradient components are discussed. Develop improved estimates of Earth surface gradients fields in the different ecosystems is considered. Copyright © 2019 for this paper by its authors. Use permitted under Creative Commons License Attribution 4.0 International (CC BY 4.0).

Scopus
Держатели документа:
Institute of Biophysics SB RAS, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Federal Research Center KSC SB RAS, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Kartushinsky, A. V.; Ogorodov, N. A.; Larko, A. A.

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10.

The use of bioluminescent biotests for study of natural and laboratory aquatic ecosystems / V. A. Kratasyuk [et al.] // Chemosphere. - 2001. - Vol. 42, Is. 8. - P909-915, DOI 10.1016/S0045-6535(00)00177-6 . - ISSN 0045-6535
Кл.слова (ненормированные):
Alcohol dehydrogenase -- Bacterial luciferase -- Bioluminescence -- Blooming -- Pollution -- Trypsin -- Water toxicity -- alcohol dehydrogenase -- benzoquinone -- luciferase -- trypsin -- aquatic ecosystem -- bioluminescence -- water quality -- article -- bacterium culture -- bioluminescence -- blue green alga -- ecosystem -- pond -- seasonal variation -- water pollution -- water quality -- Benzoquinones -- Biological Assay -- Cyanobacteria -- Ecosystem -- Environmental Monitoring -- Eutrophication -- FMN Reductase -- Indicators and Reagents -- Luminescent Measurements -- NADH, NADPH Oxidoreductases -- Water Pollutants -- Russian Federation -- algae -- Bacteria (microorganisms) -- Chlorophyta -- Cyanobacteria -- uncultured cyanobacterium
Аннотация: A set of bioluminescent tests was developed to monitor water quality in natural and laboratory ecosystems. It consisted of four bioluminescent systems: luminous bacteria, coupled enzyme system NADH:FMN-oxidoreductase-luciferase and triplet enzyme systems with alcohol dehydrogenase and trypsin. The set of biotests was applied for a small forest pond (Siberia, Russia), laboratory microecosystems polluted with benzoquinone and a batch culture of blue-green algae. Thereby effects of natural water compared to those of models of heavy pollution and "bloom" of blue-greens on the bioluminescent tests were revealed. The set of biotests was not affected by a natural seasonal variability of water quality in the unpolluted pond, but responded to the heavy pollution and the "bloom" of blue-greens. The set of biotests could be recommended as the alarm test to control the acute toxicity of natural water bodies. В© 2001 Elsevier Science Ltd.

Scopus
Держатели документа:
Krasnoyarsk State University, pr. Svobodnii 79, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Krasnoyarsk State Agricultural University, Mira av., 88, Krasnoyarsk, 660049, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kratasyuk, V.A.; Esimbekova, E.N.; Gladyshev, M.I.; Khromichek, E.B.; Kuznetsov, A.M.; Ivanova, E.A.

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11.

The smallest natural high-active luciferase: Cloning and characterization of novel 16.5-kDa luciferase from copepod Metridia longa [Text] / S. V. Markova [et al.] // Biochem. Biophys. Res. Commun. - 2015. - Vol. 457, Is. 1. - P77-82, DOI 10.1016/j.bbrc.2014.12.082. - Cited References:20. - The cloning of cDNA encoding MLuc7 luciferase of M. longa was supported by Bayer AG (Germany); all other studies - by the grant 14-14-01119 of the Russian Science Foundation. We declare that authors have no conflict of interest. . - ISSN 0006-291X. - ISSN 1090-2104

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
CDNA CLONING
   SECRETED LUCIFERASE
   ESCHERICHIA-COLI
   EXPRESSION
Кл.слова (ненормированные):
Bioluminescence -- Coelenterazine -- Copepod luciferase -- Mammalian -- expression -- Real-time imaging
Аннотация: Coelenterazine-dependent copepod luciferases containing natural signal peptide for secretion are a very convenient analytical tool as they enable monitoring of intracellular events with high sensitivity, without destroying cells or tissues. This property is well suited for application in biomedical research and development of cell-based assays for high throughput screening. We report the cloning of cDNA gene encoding a novel secreted non-allelic 16.5-kDa isoform (MLuc7) of Metridia longa luciferase, which, in fact, is the smallest natural luciferase of known for today. Despite the small size, isoform contains 10 conservative Cys residues suggesting the presence of up to 5 S-S bonds. This hampers the efficient production of functionally active recombinant luciferase in bacterial expression systems. With the use of the baculovirus expression system, we produced substantial amounts of the proper folded MLuc7 luciferase with a yield of similar to 3 mg/L of a high purity protein. We demonstrate that MLuc7 produced in insect cells is highly active and extremely thermostable, and is well suited as a secreted reporter when expressed in mammalian cells ensuring higher sensitivity of detection as compared to another Metridia luciferase isoform (MLuc164) which is widely employed in real-time imaging. (C) 2014 Elsevier Inc. All rights reserved.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Photobiol Lab, Siberian Branch, Krasnoyarsk, Russia.
Siberian Fed Univ, Chair Biophys, Krasnoyarsk, Russia.
ИБФ СО РАН

Доп.точки доступа:
Markova, Svetlana V.; Larionova, Marina D.; Burakova, Ludmila P.; Vysotski, Eugene S.; Bayer AG (Germany); Russian Science Foundation [14-14-01119]

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12.

The light-sensitive photoprotein berovin from the bioluminescent ctenophore Beroe abyssicola: a novel type of Ca2+-regulated photoprotein / S. V. Markova [et al.] // FEBS J. - 2012. - Vol. 279, Is. 5. - P856-870, DOI 10.1111/j.1742-4658.2012.08476.x. - Cited References: 63. - The authors thank Natalia Chervyakova from Department of Zoology of Invertebrates of Moscow State University for the photo of the White Sea ctenophore Beroe abyssicola. This work was supported by RFBR grant 09-04-00172, by grant 64987.2010.4, Molecular and Cellular Biology program of RAS, and Bayer Pharma AG (Germany). . - ISSN 1742-464X

РУБ Biochemistry & Molecular Biology
Рубрики:
CALCIUM-ACTIVATED PHOTOPROTEINS
   C-TERMINAL PROLINE
   SEQUENCE-ANALYSIS
   MNEMIOPSIS-SP
   COELENTERAZINE-BINDING
   ANGSTROM RESOLUTION
   RECOMBINANT OBELIN
   CRYSTAL-STRUCTURES
   EXCITED-STATE
   CDNA CLONING
Кл.слова (ненормированные):
bioluminescence -- calcium -- coelenterazine -- luciferase -- mammalian expression
Аннотация: Light-sensitive Ca2+-regulated photoproteins are responsible for the bright bioluminescence of ctenophores. Using functional screening, four full-size cDNA genes encoding the same 208-amino-acid polypeptide were isolated from two independent cDNA libraries prepared from two Beroe abyssicola specimens. Sequence analysis revealed three canonical EF-hand calcium-binding sites characteristic of Ca2+-regulated photoproteins, but a very low degree of sequence identity (2729%) with aequorin-type photoproteins, despite functional similarities. Recombinant berovin was expressed in Escherichia coli cells, purified, converted to active photoprotein and characterized. Active berovin has absorption maxima at 280 and 437 nm. The Ca2+-discharged protein loses visible absorption, but exhibits a new absorption maximum at 335 nm. The berovin bioluminescence is blue (?max = 491 nm) and a change in pH over the range 6.09.5 has no significant effect on the light emission spectrum. By contrast, the fluorescence of Ca2+-discharged protein (?ex = 350 nm) is pH sensitive: at neutral pH the maximum is at 420 nm and at alkaline pH there are two maxima at 410 and 485 nm. Like native ctenophore photoproteins, recombinant berovin is also inactivated by light. The Ca2+ concentrationeffect curve is a sigmoid with a slope on a loglog plot of similar to 2.5. Although this curve for berovin is very similar to those obtained for obelin and aequorin, there are evident distinctions: berovin responds to calcium changes at lower concentrations than jellyfish photoproteins and its Ca2+-independent luminescence is low. Recombinant berovin was successfully expressed in mammalian cells, thereby demonstrating potential for monitoring intracellular calcium.

Держатели документа:
[Vysotski, Eugene S.] Russian Acad Sci, Siberian Branch, Inst Biophys, Photobiol Lab, Krasnoyarsk 660036, Russia
[Markova, Svetlana V.
Burakova, Ludmila P.
Malikova, Natalia P.
Frank, Ludmila A.
Vysotski, Eugene S.] Siberian Fed Univ, Dept Biophys, Krasnoyarsk, Russia
[Golz, Stefan] Bayer Pharma AG, Global Drug Discovery, Wuppertal, Germany
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Markova, S.V.; Burakova, L.P.; Golz, S...; Malikova, N.P.; Frank, L.A.; Vysotski, E.S.

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13.

The Information Content of Spectral Vegetation Indices in the Interpretation of Satellite Images of Cultivated Fields / T. I. Pisman [et al.] // Biophysics. - 2019. - Vol. 64, Is. 4. - P588-592, DOI 10.1134/S0006350919040158 . - ISSN 0006-3509
Кл.слова (ненормированные):
bare fallows -- Keywords: sod fields -- NDSI -- NDVI -- Sentinel-2
Аннотация: Abstract—The results of satellite monitoring of vegetation on unused agricultural lands during the growing season of 2018 are presented. Sod fields of different ages (2, 7, and 20 years) and bare fallows on the land used by the Krasnoyarsk Research Institute of Agriculture were the objects of the study. Satellite data with high spatial resolution (Sentinel-2 Earth remote sensing satellites) at the pre-processing Level-1C (https://earthexplorer.usgs.gov/) were used for the interpretation of sod field and fallow images. These data were used to calculate the Normalized Difference Vegetation Index (NDVI) and the Normalized Difference Soil Index (NDSI). Algorithms and software for the processing of Sentinel-2 satellite data were developed. The possibility of using NDVI dynamics for assessment and monitoring of the condition of sod fields and bare fallows has been demonstrated. The applicability of the NDSI soil index for assessment of the status of arable land has been demonstrated. © 2019, Pleiades Publishing, Inc.

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Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, 50/50, Krasnoyarsk, 660036, Russian Federation
Agricultural Research Institute, Svobodnyi pr., 66, Krasnoyarsk, 660041, Russian Federation

Доп.точки доступа:
Pisman, T. I.; Shevyrnogov, A. P.; Larko, A. A.; Botvich, I. Y.; Emelyanov, D. V.; Shpedt, A. A.; Trubnikov, Y. N.

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14.

The influence of quinones and phenols on the triple NAD(H)-dependent enzyme systems [Text] / N. S. Kudryasheva [et al.] // Chemosphere. - 1999. - Vol. 38, Is. 4. - P. 751-758, DOI 10.1016/S0045-6535(98)00218-5. - Cited References: 7 . - ISSN 0045-6535

РУБ Environmental Sciences

Аннотация: Kinetics of the triple bioluminescent enzyme system: alcohol dehydrogenase - NADH:FMN-oxidoreductase - luciferase in the presence of quinones and phenols has been studied. The correspondence between the bioluminescent kinetic parameters, redox potentials and concentrations of the quinones and phenols has been estimated. The substances have been shown to change bioluminescent kinetics through moving off the NAD(+)/NADH balance in the enzyme processes. This system is proposed to be used as enzymatic biotest in ecological monitoring. (C) 1998 Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia
Irkutsk State Univ, Biol Res Inst, Irkutsk 664003, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kudryasheva, N.S.; Kudinova, I.Y.; Esimbekova, E.N.; Kratasyuk, V.A.; Stom, D.I.

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15.

The fluorescence method for determining of photosynthetic apparatus reactivity in plant leaves / T. V. Nesterenko, V. N. Shikhov, A. A. Tikhomirov // Zhurnal Obshchei Biol. - 2019. - Vol. 80, Is. 3. - С. 187-199, DOI 10.1134/S0044459619030060. - Cited References:31 . - ISSN 0044-4596

РУБ Biology
Рубрики:
ELECTRIC-POTENTIAL DIFFERENCE
FREQUENTLY ASKED QUESTIONS
CHLOROPHYLL
Аннотация: Presently, the most promising way of studying, forecasting, and enhancing of organisms' tolerance to harsh environmental impacts is considered to be the estimation of initial functional state of an organism's regulatory systems. To resolve the problem of photosynthetic apparatus (PSA) tolerance to harsh impacts at the level of such a complicated functional system as a plant leaf, it is necessary to assess integral responses of the leaf's PSA to the impact. At that, simple and versatile traits may have certain advantages. At present, chlorophyll fluorescence seems to be one of the main indices of PSA activity, which can be measured relatively fast and easy. One of the possible approaches to operational integrative assessment of PSA activity may consist in usage of the parameters introduced for the curves of chlorophyll fluorescence induction (CFI) slow phase. Temporal patterns of CFI are of special interest. The simplest index T-0.5 (i.e., half-time of chlorophyll fluorescence intensity decrease during the slow phase of CFI) appears to be an integral characteristic of activation rate with regard to a number of photo-assimilation and photo-protective processes in leaves. On basis of the studies, conducted earlier, and published data, we have analyzed the behavior of T-0.5 parameter with comparison to other CFI traits (namely: qN - non-photochemical quenching coefficient, ETR - electron transport rate, Phi(PSII) - effective quantum yield of PSII photochemistry, F-p/F-T ratio) under different conditions. The influence of leaf senescence, changes in intensity of excitation light, slight dehydration of plants and their recovery from water deficiency have been examined. The pattern of T-0.5 behavior, observed in laboratory experiments, and the results of its comparison with other indices of CFI give occasion to propose the usage of T-0.5 for indirect estimation of PSA activity when operational integrative monitoring of PSA state is required. Further studies are necessary for establishing quantitative relationships between PSA activity and fluorescence parameter T-0.5 under specific stress conditions.

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Держатели документа:
RAS, Siberian Branch, Inst Biophys, Acad Gorodok 50-50, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Nesterenko, T. V.; Shikhov, V. N.; Tikhomirov, A. A.

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16.

THE ECOLOGICAL BIOPHYSICS AND ITS PART IN INVESTIGATION OF AQUATIC ECOSYSTEMS [Текст] / I. I. GITELSON [и др.] // Biofizika. - 1993. - Vol. 38, Is. 6. - С. 1069-1078. - Cited References: 11 . - 10. - ISSN 0006-3029

РУБ Biophysics
Рубрики:
SEASONAL DYNAMICS
RESERVOIR
BAY
Аннотация: The notion of ecological biophysics as a scientific discipline investigating physical processes and phenomena caused by functioning of the living super-organism systems is formulated. The three main constituents of the ecological biophysics are defined: elaboration of the monitoring methods of the basis of sensing of the biophysical fields (of bioluminescence and fluorescence), mathematical and physical modeling and investigation of the part of living organisms in the hydrophysical processes of the ecosystem scale. Examples of realization of the ecological biophysical approach in the process of investigations of the World Ocean, Lake Baikal, the reservoirs of Dnieper and Yenisei revers are given.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
GITELSON, I.I.; GLADYSHEV, M.I.; DEGERMENDZHY, A.G.; LEVIN, L.A.; SIDKO, F.Y.

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17.

The Ca2+-Regulated Photoprotein Obelin as a Tool for SELEX Monitoring and DNA Aptamer Affinity Evaluation / V. V. Krasitskaya, N. S. Goncharova, V. V. Biriukov [et al.] // Photochem. Photobiol. - 2020, DOI 10.1111/php.13274. - Cited References:25. - This work has been supported by the Russian Foundation for Basic Research (RFBR) under the grant no 18-38-00531. . - Article in press. - ISSN 0031-8655. - ISSN 1751-1097

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
CARDIAC TROPONIN-I
   BIOLUMINESCENCE
   IMMUNOASSAY
   APTASENSOR
   DIAGNOSIS
Аннотация: Bioluminescent solid-phase analysis was proposed to monitor the selection process and to determine binding characteristics of the aptamer-target complexes during design and development of the specific aptamers. The assay involves Ca2+-regulated photoprotein obelin as a simple, sensitive and fast reporter. Applicability and the prospects of the approach were exemplified by identification of DNA aptamers to cardiac troponin I, a highly specific early biomarker for acute myocardial infarction. Two structurally different aptamers specific to various epitopes of troponin I were obtained and then tested in a model bioluminescent assay.

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Держатели документа:
Fed Res Ctr KSC SB RAS, Inst Biophys SB RAS, Krasnoyarsk, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.
Inst Chem Biol & Fundamental Med SB RAS, Novosibirsk, Russia.
Fed Res Ctr KSC SB RAS, Kirensky Inst Phys, Krasnoyarsk, Russia.

Доп.точки доступа:
Krasitskaya, Vasilisa V.; Goncharova, Natalia S.; Biriukov, Vladislav V.; Bashmakova, Eugenia E.; Kabilov, Marsel R.; Baykov, Ivan K.; Sokolov, Aleksey E.; Frank, Ludmila A.; Russian Foundation for Basic Research (RFBR)Russian Foundation for Basic Research (RFBR) [18-38-00531]

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18.

Spatial and temporal variation in Arctic freshwater chemistry-Reflecting climate-induced landscape alterations and a changing template for biodiversity / B. J. Huser, M. N. Futter, D. Bogan [et al.] // Freshw. Biol. - 2020, DOI 10.1111/fwb.13645. - Cited References:98. - Environment and Climate Change Canada; Cumulative Impact Monitoring Program, Government of Northwest Territories . - Article in press. - ISSN 0046-5070. - ISSN 1365-2427

РУБ Ecology + Marine & Freshwater Biology
Рубрики:
DISSOLVED ORGANIC-CARBON
PERMAFROST THAW
CHEMICAL LIMNOLOGY
Кл.слова (ненормированные):
biogeochemistry -- eutrophication -- lakes -- oligotrophication -- rivers
Аннотация: Freshwater chemistry across the circumpolar region was characterised using a pan-Arctic data set from 1,032 lake and 482 river stations. Temporal trends were estimated for Early (1970-1985), Middle (1986-2000), and Late (2001-2015) periods. Spatial patterns were assessed using data collected since 2001. Alkalinity, pH, conductivity, sulfate, chloride, sodium, calcium, and magnesium (major ions) were generally higher in the northern-most Arctic regions than in the Near Arctic (southern-most) region. In particular, spatial patterns in pH, alkalinity, calcium, and magnesium appeared to reflect underlying geology, with more alkaline waters in the High Arctic and Sub Arctic, where sedimentary bedrock dominated. Carbon and nutrients displayed latitudinal trends, with lower levels of dissolved organic carbon (DOC), total nitrogen, and (to a lesser extent) total phosphorus (TP) in the High and Low Arctic than at lower latitudes. Significantly higher nutrient levels were observed in systems impacted by permafrost thaw slumps. Bulk temporal trends indicated that TP was higher during the Late period in the High Arctic, whereas it was lower in the Near Arctic. In contrast, DOC and total nitrogen were both lower during the Late period in the High Arctic sites. Major ion concentrations were higher in the Near, Sub, and Low Arctic during the Late period, but the opposite bulk trend was found in the High Arctic. Significant pan-Arctic temporal trends were detected for all variables, with the most prevalent being negative TP trends in the Near and Sub Arctic, and positive trends in the High and Low Arctic (mean trends ranged from +0.57%/year in the High/Low Arctic to -2.2%/year in the Near Arctic), indicating widespread nutrient enrichment at higher latitudes and oligotrophication at lower latitudes. The divergent P trends across regions may be explained by changes in deposition and climate, causing decreased catchment transport of P in the south (e.g. increased soil binding and trapping in terrestrial vegetation) and increased P availability in the north (deepening of the active layer of the permafrost and soil/sediment sloughing). Other changes in concentrations of major ions and DOC were consistent with projected effects of ongoing climate change. Given the ongoing warming across the Arctic, these region-specific changes are likely to have even greater effects on Arctic water quality, biota, ecosystem function and services, and human well-being in the future.

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Держатели документа:
Swedish Univ Agr Sci, Dept Aquat Sci & Assessment, Box 7050, S-75007 Uppsala, Sweden.
Univ Alaska Anchorage, Alaska Ctr Conservat Sci, Anchorage, AK USA.
Norwegian Water Resources & Energy Directorate, Oslo, Norway.
Univ Oslo, Nat Hist Museum, Oslo, Norway.
Wilfrid Laurier Univ, Cold Regions Res Ctr, Waterloo, ON, Canada.
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk, Russia.
Umea Univ, Climate Impacts Res Ctr, Dept Ecol & Environm Sci, Umea, Sweden.
Queens Univ, Dept Biol, Paleoecol Environm Assessment & Res Lab PEARL, Kingston, ON, Canada.
Norwegian Inst Nat Res, Oslo, Norway.
Univ New Brunswick, Canadian Rivers Inst, Fredericton, NB, Canada.
Univ New Brunswick, Dept Biol, Fredericton, NB, Canada.

Доп.точки доступа:
Huser, Brian J.; Futter, Martyn N.; Bogan, Daniel; Brittain, John E.; Culp, Joseph M.; Goedkoop, Willem; Gribovskaya, Iliada; Karlsson, Jan; Lau, Danny C. P.; Ruhland, Kathleen M.; Schartau, Ann Kristin; Shaftel, Rebecca; Smol, John P.; Vrede, Tobias; Lento, Jennifer; Environment and Climate Change Canada; Cumulative Impact Monitoring Program, Government of Northwest Territories

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19.

Software for matching standard activity enzyme biosensors for soil pollution analysis / V. A. Kratasyuk, E. M. Kolosova, O. S. Sutormin [et al.] // Sensors. - 2021. - Vol. 21, Is. 3. - Ст. 1017. - P1-10, DOI 10.3390/s21031017 . - ISSN 1424-8220
Кл.слова (ненормированные):
Bacterial luciferase -- Biosensors -- Butyrylcholinesterase -- Enzyme -- Lactic dehydrogenase -- Software -- Soil pollution -- Biosensors -- Soil pollution -- Soil surveys -- Soils -- Commercial standards -- Environmental Monitoring -- Enzyme biosensors -- Enzyme systems -- Inhibitory effect -- JavaScript programming -- Soil sample -- Toxic agents -- Enzyme activity
Аннотация: This work is dedicated to developing enzyme biosensor software to solve problems regarding soil pollution analysis. An algorithm and specialised software have been developed which stores, analyses and visualises data using JavaScript programming language. The developed software is based on matching data of 51 non-commercial standard soil samples and their inhibitory effects on three enzyme systems of varying complexity. This approach is able to identify the influence of chemical properties soil samples, without toxic agents, on enzyme biosensors. Such software may find wide use in environmental monitoring. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Scopus
Держатели документа:
Department of Biophysics, Institute of Fundamental Biology and Biotechology, Siberian Federal University, 79 Svobodny pr, Krasnoyarsk, 660041, Russian Federation
Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, Photobiology Laboratory, Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, 50/50 Akagemgorodok, Krasnoyarsk, 660036, Russian Federation
Department of High-Efficiency Calculations, Siberian Federal University, 26-ULK building Kirensky St, Krasnoyarsk, 660074, Russian Federation
Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, Krasnoyarsk Research Institute of Agriculture, Russian Academy of Sciences, Siberian Branch, 66 Svobodny pr, Krasnoyarsk, 660037, Russian Federation
Federal Research Center ‘Krasnoyarsk Scientific Center SB RAS’, Krasnoyarsk Research Institute of Agricultural, Siberian Federal University, 79 Svobodny pr, Krasnoyarsk, 660041, Russian Federation

Доп.точки доступа:
Kratasyuk, V. A.; Kolosova, E. M.; Sutormin, O. S.; Lonshakova-Mukina, V. I.; Baygin, M. M.; Rimatskaya, N. V.; Sukovataya, I. E.; Shpedt, A. A.

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20.

Software for Matching Standard Activity Enzyme Biosensors for Soil Pollution Analysis / V. A. Kratasyuk, E. M. Kolosova, O. S. Sutormin [et al.] // Sensors. - 2021. - Vol. 21, Is. 3. - Ст. 1017, DOI 10.3390/s21031017. - Cited References:20. - This research was funded by RFBR, Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science, Grant number 20-44-243001 and the Ministry of Science and Higher Education of the Russian Federation, Grant number FSRZ-2020-0006. . - ISSN 1424-8220

РУБ Chemistry, Analytical + Engineering, Electrical & Electronic + Instruments

Кл.слова (ненормированные):
biosensors -- enzyme -- butyrylcholinesterase -- lactic dehydrogenase -- bacterial luciferase -- soil pollution -- software
Аннотация: This work is dedicated to developing enzyme biosensor software to solve problems regarding soil pollution analysis. An algorithm and specialised software have been developed which stores, analyses and visualises data using JavaScript programming language. The developed software is based on matching data of 51 non-commercial standard soil samples and their inhibitory effects on three enzyme systems of varying complexity. This approach is able to identify the influence of chemical properties soil samples, without toxic agents, on enzyme biosensors. Such software may find wide use in environmental monitoring.

WOS
Держатели документа:
Siberian Fed Univ, Inst Fundamental Biol & Biotechol, Dept Biophys, 79 Svobodny Pr, Krasnoyarsk 660041, Russia.
Russian Acad Sci, Krasnoyarsk Sci Ctr SB RAS, Photobiol Lab, Fed Res Ctr,Siberian Branch,Inst Biophys, 50-50 Akagemgorodok, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Dept High Efficiency Calculat, 26 ULK Bldg Kirensky St, Krasnoyarsk 660074, Russia.
Russian Acad Sci, Krasnoyarsk Sci Ctr SB RAS, Krasnoyarsk Res Inst Agr, Siberian Branch,Fed Res Ctr, 66 Svobodny Pr, Krasnoyarsk 660037, Russia.
Siberian Fed Univ, Inst Fundamental Biol & Biotechol, Dept Aquat & Terr Ecosyst, 79 Svobodny Pr, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Kratasyuk, Valentina A.; Kolosova, Elizaveta M.; Sutormin, Oleg S.; Lonshakova-Mukina, Viktoriya, I; Baygin, Matvey M.; Rimatskaya, Nadezhda, V; Sukovataya, Irina E.; Shpedt, Alexander A.; RFBRRussian Foundation for Basic Research (RFBR); Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science [20-44-243001]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]

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