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THE ECOLOGICAL BIOPHYSICS AND ITS PART IN INVESTIGATION OF AQUATIC ECOSYSTEMS [Текст] / I. I. GITELSON [и др.] // Biofizika. - 1993. - Vol. 38, Is. 6. - С. 1069-1078. - Cited References: 11 . - 10. - ISSN 0006-3029

РУБ Biophysics
Рубрики:
SEASONAL DYNAMICS
RESERVOIR
BAY
Аннотация: The notion of ecological biophysics as a scientific discipline investigating physical processes and phenomena caused by functioning of the living super-organism systems is formulated. The three main constituents of the ecological biophysics are defined: elaboration of the monitoring methods of the basis of sensing of the biophysical fields (of bioluminescence and fluorescence), mathematical and physical modeling and investigation of the part of living organisms in the hydrophysical processes of the ecosystem scale. Examples of realization of the ecological biophysical approach in the process of investigations of the World Ocean, Lake Baikal, the reservoirs of Dnieper and Yenisei revers are given.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
GITELSON, I.I.; GLADYSHEV, M.I.; DEGERMENDZHY, A.G.; LEVIN, L.A.; SIDKO, F.Y.

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Ecological biophysics and its role in study of aquatic ecosystems / I. I. Gitel'zon [et al.] // Biophysics. - 1993. - Vol. 38, Is. 6. - P1099-1108 . - ISSN 0006-3509
Аннотация: A concept is formulated on ecological and biophysics as a scientific discipline which studies physical processes and phenomena resulting from the functioning of live supra-organism systems. Three main directions of ecological biophysics are defined: creation of methods of monitoring ecosystems on the basis of probing biophysical fields (fields of bioluminescence and fluorescence); mathematical and physical modelling; and study of the role of live organisms in hydrophysical processes on the ecosystem scale. Examples are given of the realization of the ecologo-biophysical approach in research into the ecosystems of the world ocean, Lake Baikal and the Dnieper and Yenisei reservoirs. В© 1994.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitel'zon, I.I.; Gladyshev, M.I.; Degermendzhi, A.G.; Levin, L.A.; Sid'ko, F.Ya.

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Remote diagnostics of vegetation covers by spectral brightness of plants / A. P. Shevyrnogov, A. F. Sidko // Advances in Space Research. - 1995. - Vol. 16, Is. 10. - P185-188 . - ISSN 0273-1177
Кл.слова (ненормированные):
plant -- spectral brightness -- vegetation
Аннотация: Optical remote methods are able to solve numerous scientific and practical problems associated with development of ecological monitoring systems, monitoring of plant cenoses' condition, forests and agricultural crops, in particular. Investigation of vegetation covers (VC) by optical remote methods is based on comprehensive examination and analysis of interaction mechanisms of incident and reflected solar light with plants, their dependence on geometric and pigment structure. В© 1995.

Scopus
Держатели документа:
Institute of Biophysics (Russian Academy of Sciences, Siberian Branch), Academgorodok, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shevyrnogov, A.P.; Sidko, A.F.

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Rapid assay of fatty acid composition using a portable high-performance liquid chromatograph for monitoring aquatic ecosystems / N. N. Sushchik [et al.] // Journal of Chromatography A. - 1995. - Vol. 695, Is. 2. - P223-228, DOI 10.1016/0021-9673(94)01090-2 . - ISSN 0021-9673
Кл.слова (ненормированные):
fatty acid -- alga -- article -- culture medium -- ecology -- high performance liquid chromatography -- instrument -- methodology -- priority journal -- ultraviolet spectrophotometry -- water analysis
Аннотация: The chromatographic conditions presented allowed the separation of the nitrophenacyl derivatives of standards of eleven free fatty acids (FFA) using a portable high-performance chromatograph, suitable for use aboard a research vessel. A statistically significant linear correlation between UV absorbance and amount of the analytes injected was obtained. The method was tested on FFA from algae cultural media. The method can be used for the ecological monitoring of natural waters.

Scopus
Держатели документа:
Institute Biophysics, Akademgorodok, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sushchik, N.N.; Gladyshev, M.I.; Kalachova, G.S.; Guseynova, V.E.

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REMOTE DIAGNOSTICS OF VEGETATION COVERS BY SPECTRAL BRIGHTNESS OF PLANTS [Text] / A. P. SHEVYRNOGOV, A. F. SIDKO ; ed. OV, AP SHEVYRN // SATELLITE MONITORING OF THE EARTH'S SURFACE AND ATMOSPHERE. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON PRESS LTD, 1995. - Vol. 16: A1 Symposium of COSPAR Scientific Commission A on Satellite Monitoring of the Earths Surface and Atmosphere, at the 13th COSPAR Scientific Assembly (JUL 11-21, 1994, HAMBURG, GERMANY), Is. 10. - P185-188, DOI 10.1016/0273-1177(95)00401-Y. - Cited References: 5 . - 4. - ISBN 0273-1177. - ISBN 0-08-042635-2

РУБ Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences + Remote Sensing

Аннотация: Optical remote methods are able to solve numerous scientific and practical problems associated with development of ecological monitoring systems, monitoring of plant cenoses' condition, forests and agricultural crops, in particular. Investigation of vegetation covers (VC) by optical remote methods is based on comprehensive examination and analysis of interaction mechanisms of incident and reflected solar light with plants, their dependence on geometric and pigment structure.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
SHEVYRNOGOV, A.P.; SIDKO, A.F.; SHEVYRN, OV, AP \ed.\

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Observed trends of chlorophyll concentration in the surface layer of the Northern and Central Atlantic (1979-1983) [Text] / A. P. Shevyrnogov, G. S. Vysotskaya ; ed. BJ Choudhury [et al.] // SYNERGISTIC USE OF MULTISENSOR DATA FOR LAND PROCESSES. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON PRESS LTD, 1998. - Vol. 22: A3 1 Symposium of COSPAR Scientific Commission A on Synergistic Use of Multisensor Data for Land Processes at 31st COSPAR Scientific Assembly (JUL 14-21, 1996, BIRMINGHAM, ENGLAND), Is. 5. - P. 701-704, DOI 10.1016/S0273-1177(97)01136-8. - Cited References: 7 . - ISBN 0273-1177. - ISBN 0-08-043469-X

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences + Remote Sensing

Аннотация: To preserve and utilize the biosphere it is imperative that we understand the long-term dynamics of the primary production process on our planet. Variability of chlorophyll concentration in the ocean is one of the most important components of this process. However, size and access make its investigation labour-intensive. Dependence of primary production on high variability of hydrophysical phenomena in the ocean (fluctuations of currents, frontal zones, etc.) makes it difficult to locate points to measure chlorophyll concentration dynamics. To locate such quasistationary zones with respect to seasonal dynamics of chlorophyll concentration by satellite data the Institute of Biophysics (Russian Academy of Sciences, Siberian Branch) has developed a geoinformation system. II use made it possible to locate such zones over the entire ocean. The work shows zones on the Central and Northern Atlantic. They form the ground to analyse perennial dynamics of chlorophyll concentration. The quasistationary zones are proposed for systematic monitoring of phytopigment concentration by space-borne and marine craft. (C) 1998 COSPAR. Published by Elsevier Science Ltd.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
Russian Acad Sci, Siberian Branch, Ctr Comp, Krasnoyarsk 660036, Russia
ИБФ СО РАН
ИВМ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shevyrnogov, A.P.; Vysotskaya, G.S.; Choudhury, BJ \ed.\; Tanaka, S \ed.\; Kondo, A \ed.\; Menenti, M \ed.\; Menent, M \ed.\

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Observed trends of chlorophyll concentration in the surface layer of the northern and central Atlantic (1979-1983) / A. P. Shevyrnogov, G. S. Vysotskaya // Advances in Space Research. - 1998. - Vol. 22, Is. 5. - P701-704, DOI 10.1016/S0273-1177(97)01136-8 . - ISSN 0273-1177
Кл.слова (ненормированные):
chlorophyll -- primary production -- remote sensing -- Atlantic Ocean
Аннотация: To preserve and utilize the biosphere it is imperative that we understand the long-term dynamics of the primary production process on our planet. Variability of chlorophyll concentration in the ocean is one of the most important components of this process. However, size and access make its investigation labour-intensive. Dependence of primary production on high variability of hydrophysical phenomena in the ocean (fluctuations of currents, frontal zones, etc.) makes it difficult to locate points to measure chlorophyll concentration dynamics. To locate such quasistationary zones with respect to seasonal dynamics of chlorophyll concentration by satellite data the Institute of Biophysics (Russian Academy of Sciences, Siberian Branch) has developed a geoinformation system. Its use made it possible to locate such zones over the entire ocean. The work shows zones on the Central and Northern Atlantic. They form the ground to analyse perennial dynamics of chlorophyll concentration. The quasistationary zones are proposed for systematic monitoring of phytopigment concentration by space-borne and marine craft.

Scopus
Держатели документа:
Institue of Biophysics, Siberian Branch, Russian Academy of Sciences, Academgorodok 660036, Krasnoyarsk, Russian Federation
Computer Center, Siberian Branch, Russian Academy of Sciences, Academgorodok 660036, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shevyrnogov, A.P.; Vysotskaya, G.S.

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Development of bioluminescent bioindicators for analysis of environmental pollution [Text] / N. . Kudryasheva [et al.] // Field Anal. Chem. Technol. - 1998. - Vol. 2, Is. 5. - P. 277-280, DOI 10.1002/(SICI)1520-6521(1998)2:5277::AID-FACT43.0.CO;2-P. - Cited References: 17 . - ISSN 1086-900X

РУБ Chemistry, Analytical + Environmental Sciences + Instruments & Instrumentation

Кл.слова (ненормированные):
biotest -- bioluminescence -- enzymes -- environmental monitoring
Аннотация: The influence of several suites of pollutants (metallic salts, quinones, and phenols) on bacterial bioluminescence in vivo and in vitro (five test systems) was investigated. The sensitivity of bioluminescence to the different pollutants was evaluated, and inhibition constants were measured. The data obtained were shown to correlate with the physical and chemical characteristics of the substances and the structure of the bioluminescent systems. It has been found that three bioluminescent tests (water-soluble enzyme systems, immobilized enzyme systems, and bioluminescent bacteria) show higher sensitivity to pollutants and cover all types of widespread contamination. These tests were chosen as a set of bioluminescent assays for the detection of pollutants. (C) 1998 John Wiley & Sons, Inc.

WOS
Держатели документа:
RAS, Inst Biophys, SB, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kudryasheva, N...; Kratasyuk, V...; Esimbekova, E...; Vetrova, E...; Nemtseva, E...; Kudinova, I...

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Biotesting of Effluent and River Water by Lyophilized Luminous Bacteria Biotest / A. M. Kuznetsov, E. K. Rodicheva, S. E. Medvedeva // Field Analytical Chemistry and Technology. - 1998. - Vol. 2, Is. 5. - P267-275 . - ISSN 1086-900X
Кл.слова (ненормированные):
Bioluminescence -- Effluent -- Luminous bacteria biotest -- Toxicity -- Water quality
Аннотация: Waters of the Yenisei River, certain rivers and lakes of the Altai Territory, and effluents of some industrial factories in Krasnoyarsk were studied by luminous bacteria biotest Microbiosensor B17 677F. The lyophilized luminous bacteria Photobacterium phosphoreum from the IBSO collection were used to design this biotest. The bioluminescent test is based on bioluminescence quenching resulting from the action of water samples on luminous bacteria. The test results indicated locations and zones of impaired water quality. The heaviest pollution of water in the Yenisei River was recorded in the zones 0-116 km downstream from Krasnoyarsk (Krasnoyarsk and satellite towns). The effluents of most factories were found to be toxic. Underground and surface waters of some areas of the Altai Territory had different toxicity levels; there were deviations from the norm in most water samples taken from the different lakes and rivers. The data from this study show that the luminous bacteria biotest is simple and convenient, and that the results obtained are within acceptable levels of accuracy for the evaluation of the toxicity of effluent and river water. It takes no more than 30 min to do the biotest. It can be used in ecological monitoring like the Microtox toxicity test. В© 1998 John Wiley & Sons, Inc.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kuznetsov, A.M.; Rodicheva, E.K.; Medvedeva, S.E.

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10.

A biological luciferase test for the bioluminescent assay of wheat grain infection with Fusarium [Text] / V. A. Kratasyuk [et al.] // Appl. Biochem. Microbiol. - 1998. - Vol. 34, Is. 6. - P. 622-624. - Cited References: 7 . - ISSN 0003-6838

РУБ Biotechnology & Applied Microbiology + Microbiology

Аннотация: The extent of inhibition of the bioluminescence reaction by wheat grain extracts was studied as a function of the scabby kernel content in wheat. The NADH : flavine mononucleotide oxidoreductase-luciferase bienzyme bioluminescence system was found to be the most sensitive to mycotoxins produced by fungi of the genus Fusarium. A biological luciferase test was developed for monitoring wheat grain infection with Fusarium.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
All Russia Res Inst Grain & Grain Prod, Moscow 127434, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kratasyuk, V.A.; Egorova, O.I.; Esimbekova, E.N.; Kudryashova, N.S.; Orlova, N.Y.; L'vova, L.S.

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11.

Experimental microcosms as models of natural ecosystems for monitoring survival of genetically modified microorganism. / U - Popova LYu [et al.] // Life support & biosphere science : international journal of earth space. - 1999. - Vol. 6, Is. 3. - P193-197 . - ISSN 1069-9422
Кл.слова (ненормированные):
bacterial DNA -- recombinant DNA -- adaptation -- article -- ecosystem -- Escherichia coli -- genetics -- microbiology -- plasmid -- risk assessment -- Adaptation, Biological -- DNA, Bacterial -- DNA, Recombinant -- Ecosystem -- Escherichia coli -- Microbiology -- Plasmids -- Risk Assessment -- Soil Microbiology -- Water Microbiology
Аннотация: An experimental approach for investigation of genetically modified microorganisms (GMMO) introduced into model ecosystems to evaluate potential risk of propagation of recombinant plasmids in surrounding medium has been developed. The object of modeling was Escherichia coli Z905 strain with a recombinant plasmid with bacterial luminescence genes, which was introduced into water microcosms of different structure. The approach involves comprehensive investigation of GMMO at four hierarchical levels: molecular (retaining the structure of the plasmid and expression of cloned genes); cellular (variation of metabolic activity); population (competitive power and metabolic interactions of GMMO with indigenous microflora, migration of recombinant and natural plasmids); ecosystem (effect of GMMO and cloned genes on ecosystem parameters). The experimental evidence and theoretical estimates are intended to form grounds to develop a basic version of an ecological certificate for different GMMO variants.

Scopus
Держатели документа:
Institute of Biophysics (Russian Academy of Sciences, Siberian Branch), Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
, U - Popova LYu; Pechurkin, N.S.; Maksimova, E.E.; Kargatova, T.V.; , U - Krylova TYu; Lobova, T.I.; Boyandin, A.N.

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12.

Key factors in development of man-made and natural ecosystems / N. S. Pechurkin // Advances in Space Research. - 1999. - Vol. 24, Is. 3. - P377-381, DOI 10.1016/S0273-1177(99)00321-X . - ISSN 0273-1177
Кл.слова (ненормированные):
artificial ecosystem -- ecosystem function -- article -- ecosystem -- microbiology -- microclimate -- Ecological Systems, Closed -- Ecosystem -- Environmental Microbiology -- Life Support Systems -- Soil Microbiology
Аннотация: Key factors of ecosystem functioning are of the same nature for artificial and natural types. An hierarchical approach gives the opportunity for estimation of the quantitative behavior of both individual links and the system as a whole. At the organismic level we can use interactions of studied macroorganisms (man, animal, higher plant) with selected microorganisms as key indicating factors of the organisms immune status. The most informative factor for the population/community level is an age structure of populations and relationships of domination/elimination. The integrated key factors of the ecosystems level are productivity and rates of cycling of the limiting substances. The key factors approach is of great value for growth regulations and monitoring the state of any ecosystem, including the life support system (LSS)-type.

Scopus
Держатели документа:
Institute of Biophysics, Academgorodok, 660036, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pechurkin, N.S.

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13.

Analysis of river water by bioluminescent biotests / A. M. Kuznetsov, E. K. Rodicheva, S. E. Medvedeva // Luminescence. - 1999. - Vol. 14, Is. 5. - P263-265 . - ISSN 1522-7235
Кл.слова (ненормированные):
Bioluminescent bioassay -- Pollution -- Water quality -- fresh water -- article -- bioassay -- Escherichia coli -- freeze drying -- genetic procedures -- luminescence -- methodology -- Photobacterium -- Russian Federation -- sensitivity and specificity -- water pollutant -- Biological Assay -- Biosensing Techniques -- Escherichia coli -- Freeze Drying -- Fresh Water -- Luminescence -- Photobacterium -- Russia -- Sensitivity and Specificity -- Water Pollutants, Chemical
Аннотация: The bacterial bioluminescence has high sensitivity to the action of various inhibitors of biological activity. The lyophilized luminous bacteria Photobacterium phosphoreum (Microbiosensor B17 677F) and luminous strain Escherichia coli (Microbiosensor EC) from the Culture Collection IBSO were used to create bioluminescent biotests. They have been applied in ecological monitoring to determine the overall toxicity of the Yenisei and Angara Rivers and some water sources of Altai Territory. As a rule the heaviest pollution of water in studied rivers was registered near cities and settlements. The luminous bacteria biotests are simple and convenient in work, standardized and quantitative, have rapid response to actions of different substances and high sensitivity to environmental pollutants. It takes less than 30 min to do the biotest (the other biotests take 48-96 h). Copyright В© 1999 John Wiley & Sons, Ltd.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kuznetsov, A.M.; Rodicheva, E.K.; Medvedeva, S.E.

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14.

Bioluminescent water quality monitoring of salt lake Shira [Text] / V. A. Kratasyuk, E. V. Vetrova, N. S. Kudryasheva // Luminescence. - 1999. - Vol. 14: 10th International Symposium on Bioluminescence and Chemiluminescence (1998, BOLOGNA, ITALY), Is. 4. - P. 193-195, DOI 10.1002/(SICI)1522-7243(199907/08)14:4193::AID-BIO5283.3.CO;2-J. - Cited References: 9 . - ISSN 1522-7235

РУБ Biochemistry & Molecular Biology

Кл.слова (ненормированные):
bioluminescence -- biotest -- ecological monitoring -- salt lake
Аннотация: The coupled bioluminescent enzyme system luciferase-NADH:FMN-oxidoreductase was used as a biotest in ecological monitoring of the health resort salt lake Shira (South Siberia, Russia). The technique was adapted to saltwater conditions. Bioluminescence kinetic parameters sensitive to pollutants were determined. Conditions for the use of bacterial bioluminescence biotests in salty environmental media were established. Copyright (C) 1999 John Wiley & Sons, Ltd.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, SB, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kratasyuk, V.A.; Vetrova, E.V.; Kudryasheva, N.S.

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15.

The influence of quinones and phenols on the triple NAD(H)-dependent enzyme systems [Text] / N. S. Kudryasheva [et al.] // Chemosphere. - 1999. - Vol. 38, Is. 4. - P. 751-758, DOI 10.1016/S0045-6535(98)00218-5. - Cited References: 7 . - ISSN 0045-6535

РУБ Environmental Sciences

Аннотация: Kinetics of the triple bioluminescent enzyme system: alcohol dehydrogenase - NADH:FMN-oxidoreductase - luciferase in the presence of quinones and phenols has been studied. The correspondence between the bioluminescent kinetic parameters, redox potentials and concentrations of the quinones and phenols has been estimated. The substances have been shown to change bioluminescent kinetics through moving off the NAD(+)/NADH balance in the enzyme processes. This system is proposed to be used as enzymatic biotest in ecological monitoring. (C) 1998 Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia
Irkutsk State Univ, Biol Res Inst, Irkutsk 664003, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kudryasheva, N.S.; Kudinova, I.Y.; Esimbekova, E.N.; Kratasyuk, V.A.; Stom, D.I.

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16.

Time-space structure and variability of surface temperature frontal zones in the ocean (based on AVHRR satellite data) / A. V. Kartushinsky // Advances in Space Research. - 2000. - Vol. 25, Is. 5. - P1107-1110, DOI 10.1016/S0273-1177(99)00871-6 . - ISSN 0273-1177
Кл.слова (ненормированные):
AVHRR -- frontal feature -- global ocean -- sea surface temperature -- spatiotemporal analysis
Аннотация: Satellite monitoring of large-scale ocean fields allows collection and analyse of information on dynamics processes in various areas of the ocean. Investigation of the World Ocean processes involves the determination of space-time changes of sea surface temperature (SST). Water temperature of the ocean is one of major characteristics of surface water masses. Distribution of water temperature depends not only on regional climate peculiarities but also on formation of high gradient temperature zones. These zones emerge due to interaction of different current structure, circulation and eddies. Some more important characteristics of such zones are space dimensions and time of existence (lifetime). Space-time scales of the above structure have not been investigated in detail. The aim of the paper is to study space-time changes of the surface temperature frontal zones and frontal partitions in the ocean by AVHRR satellite information.

Scopus
Держатели документа:
Institute of Biophysics (Russian Academy of Sciences, Siberian Branch), Academgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kartushinsky, A.V.

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17.

Effects of quinones on NADH-dependent enzymatic bioluminescent systems [Text] / N. S. Kudryasheva [et al.] // Appl. Biochem. Microbiol. - 2000. - Vol. 36, Is. 4. - P. 409-413, DOI 10.1007/BF02738052. - Cited References: 13 . - ISSN 0003-6838

РУБ Biotechnology & Applied Microbiology + Microbiology

Аннотация: The effects of a number of quinones on the bioluminescence characteristics of a three-component enzymatic system containing alcohol dehydrogenase, bacterial luciferase, and NADH-FMN oxidoreductase were studied to find the most sensitive kinetic parameters of the system intended to be used in biological testing. Both direct and back reactions catalyzed by alcohol dehydrogenase were studied in the presence and in the absence of quinones. The kinetic parameters of the bioluminescent system were found to depend on the redox potentials and concentrations of quinones. The quinone-induced effects were shown to be associated with changes in the NAD(+)/NADH ratio in the chain of NADH-dependent enzymes, The three-enzyme system based on alcohol dehydrogenase is suggested as a bioluminescence test for ecological monitoring of waste water.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
Irkutsk State Univ, Irkutsk 664003, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kudryasheva, N.S.; Esimbekova, E.N.; Kudinova, I.Y.; Kratasyuk, V.A.; Stom, D.I.

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18.

On monitoring the bacterial component as an indicator of the state of small man-made ecosystems / A. B. Sarangova, L. A. Somova, N. S. Pechurkin // Advances in Space Research. - 2001. - Vol. 27, Is. 9. - P1605-1609, DOI 10.1016/S0273-1177(01)00256-3 . - ISSN 0273-1177
Кл.слова (ненормированные):
Bacteria -- Ecosystems -- Substrates -- Intracellular substrate concentration -- Space research -- catalase -- oxidoreductase -- artificial ecosystem -- article -- bacterial phenomena and functions -- biomass -- culture medium -- ecosystem -- enzymology -- growth, development and aging -- metabolism -- microbiology -- oxygen consumption -- Pseudomonas -- Bacterial Physiology -- Biomass -- Catalase -- Culture Media -- Ecosystem -- Oxidoreductases -- Oxygen Consumption -- Pseudomonas -- Water Microbiology
Аннотация: High reproduction rates make the bacterial component of ecosystems a good indicator of the state of the system on the whole. This determines the necessity to develop rapid monitoring of the functional state of the bacterial component of small ecosystems. Information about substrate concentration in the population is indicative of the state of the bacterial culture. Conventional methods of monitoring the concentration of integral substrate in the system take time much longer than the changes in the ecosystem. The paper presents theoretical foundations for the logical sequence "catalase activity - intracellular substrate concentration - estimate of substrate consumed by bacteria" for experimental verification and as a consequence of development of the integral method of monitoring the bacterial population on the basis of determining bacterial catalase activity. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

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Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Academgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sarangova, A.B.; Somova, L.A.; Pechurkin, N.S.

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19.

On monitoring the bacterial component as an indicator of the state of small man-made ecosystems [Text] / A. B. Sarangova, L. A. Somova, N. S. Pechurkin ; ed. LA Somova // SPACE LIFE SCIENCES: CLOSED ECOLOGICAL SYSTEMS: EARTH AND SPACE APPLICATIONS. Ser. ADVANCES IN SPACE RESEARCH : ELSEVIER SCIENCE BV, 2001. - Vol. 27: F4 4 Symposium of COSPAR Scientific Commission F held at the 33rd COSPAR Scientific Assembly (JUL, 2000, WARSAW, POLAND), Is. 9. - P. 1605-1609, DOI 10.1016/S0273-1177(01)00256-3. - Cited References: 16 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences

Аннотация: High reproduction rates make the bacterial component of ecosystems a good indicator of the state of the system on the whole. This determines the necessity to develop rapid monitoring of the functional state of the bacterial component of small ecosystems. Information about substrate concentration in the population is indicative of the state of the bacterial culture. Conventional methods of monitoring the concentration of integral substrate in the system take time much longer than the changes in the ecosystem. The paper presents theoretical foundations for the logical sequence "catalase activity - intracellular substrate concentration - estimate of substrate consumed by bacteria" for experimental verification and as a consequence of development of the integral method of monitoring the bacterial population on the basis of determining bacterial catalase activity. (C) 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sarangova, A.B.; Somova, L.A.; Pechurkin, N.S.; Somova, LA \ed.\

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20.

Closed artificial ecosystems as a means of ecosystem studies for earth and space needs / N. S. Pechurkin, I. M. Shirobokova // Advances in Space Research. - 2001. - Vol. 27, Is. 9. - P1497-1504, DOI 10.1016/S0273-1177(01)00244-7 . - ISSN 0273-1177
Кл.слова (ненормированные):
artificial ecosystem -- bioremediation -- biosphere -- ecosystem -- environmental monitoring -- model -- Bioremediation -- Ecology -- Ecosystems -- Health -- Biosphere -- Space research -- artificial ecosystem -- Biodegradation, Environmental -- Earth (Planet) -- Ecological Systems, Closed -- Ecology -- Ecosystem -- Energy Transfer -- Environmental Microbiology -- Life Support Systems -- Population Dynamics -- Yeasts
Аннотация: Closed Artificial ecosystems (CAES) have good prospects for wide use as new means for quantitative studies of different types of both natural ecosystems and man-made ones. The paper deals with the discussion of three points of CAES applications. The first one is of importance for theoretical ecology development and is connected with bringing together В«holisticВ» and В«merologicalВ» approaches in ecosystems studies. Using CAES, we can combine both approaches, taking into account the biotic turnover of limiting substrates which few in number even for complicated natural ecosystems. The second CAES use concerns the development of В«ecosystems healthВ» concept and application of a key-factor-approach for the indication and measurement of healthy unhealthy state and functioning of ecosystems or their links. The third use is more of an applied nature, oriented to the intensification of bioremediation or biodepollution processes in different types of ecosystems, including the global biosphere. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, 660036, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pechurkin, N.S.; Shirobokova, I.M.

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