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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prokopkin I.G., Mooij W.M., Janse J.H., Degermendzhy A.G.
Заглавие : A general one-dimensional vertical ecosystem model of Lake Shira (Russia, Khakasia): Description, parametrization and analysis
Место публикации : Aquatic Ecology. - 2010. - Vol. 44, Is. 3. - С. 585-618. - ISSN 13862588 (ISSN) , DOI 10.1007/s10452-010-9326-8
Ключевые слова (''Своб.индексиров.''): meromictic brackish lake--one-dimensional vertical ecological model--sensitivity analysis--alga--biomass--brackish water--diffusion--ecosystem modeling--meromictic lake--microbial community--model test--nutrient--one-dimensional modeling--parameterization--phytoplankton--seasonality--sensitivity analysis--solar radiation--vertical profile--water chemistry--water temperature--zooplankton--khakassia--lake shira--russian federation--algae
Аннотация: A one-dimensional ecological model of the meromictic brackish Lake Shira (Russia, Khakasia) was developed. The model incorporates state-of-the-art knowledge about the functioning of the lake ecosystem using the most recent field observations and ideas from PCLake, a general ecosystem model of shallow freshwater lakes. The model of Lake Shira presented here takes into account the vertical dynamics of biomasses of the main species of algae, zooplankton and microbial community, as well as the dynamics of oxygen, detritus, nutrients and hydrogen sulphide from spring to autumn. Solar radiation, temperature and diffusion are modelled using real meteorological data. The parameters of the model were calibrated to the field data, after applying different methods of sensitivity analysis to the model. The resulting patterns of phytoplankton and nutrients dynamics show a good qualitative and quantitative agreement with the field observations during the whole summer season. Results are less satisfactory with respect to the vertical distribution of zooplankton biomass. We hypothesize that this is due to the fact that the current model does not take the sex and age structure of zooplankton into account. The dynamics of oxygen, hydrogen sulphide and the modelled positions of the chemocline and thermocline are again in good agreement with field data. This resemblance confirms the validity of the approach we took in the model regarding the main physical, chemical and ecological processes. This general model opens the way for checking various hypotheses on the functioning of the Lake Shira ecosystem in future investigations and for analysing options for management of this economically important lake. В© 2010 Springer Science+Business Media B.V.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Rogozin D.Y., Genova S.N., Gulati R.D., Degermendzhy A.G.
Заглавие : Some generalizations based on stratification and vertical mixing in meromictic Lake Shira, Russia, in the period 2002-2009
Место публикации : Aquatic Ecology. - 2010. - Vol. 44, Is. 3. - С. 485-496. - ISSN 13862588 (ISSN) , DOI 10.1007/s10452-010-9328-6
Ключевые слова (''Своб.индексиров.''): 1-d model--meromixis--mixolimnion--oxic-anoxic interface--thermocline--weather conditions--brackish water--meromictic lake--numerical model--one-dimensional modeling--overturn--physicochemical property--salinity--seasonality--stratification--temperate environment--vertical mixing--water temperature--weather--khakassia--lake shira--russian federation
Аннотация: In a brackish, temperate, 24-m-deep Lake Shira, the profiles of salinity, temperature, oxygen and sulfide concentrations were measured on a seasonal basis from 2002 to 2009. The lake was shown to be meromictic with autumnal overturn restricted to mixolimnion. The depth of mixolimnion and position of oxic-anoxic interface varied annually. The spring mixing processes contribute to the formation of mixolimnion in autumn. The exceptionally windy spring of 2007 caused the deepening of mixolimnion in the winter of 2008. The winter position of oxic-anoxic interface was affected by the position of lower boundary of mixolimnion in all winters. The salinity in the winter mixolimnion increased compared with the autumn because of freezing out of salts from the upper water layers meters during ice formation and their dissolution in water below. The profiles of salinity and temperature were simulated by the mathematical 1-D model of temperature and salinity conditions taking into account ice formation. The simulated profiles generally coincided with the measured ones. The coincidence implies that simplified one-dimensional model can be applied to roughly describe salinity and density profiles and mixing behavior of Lake Shira. В© 2010 The Author(s).
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Degermendzhy A.G., Gulati R.D.
Заглавие : Understanding the mechanisms of blooming of phytoplankton in Lake Shira, a saline lake in Siberia (the Republic of Khakasia)
Место публикации : Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - С. 331-340. - ISSN 13862588 (ISSN)
Ключевые слова (''Своб.индексиров.''): carbon budget--cyanobacteria--heterotrophic bacteria--hydrogen sulphide--mathematical models of stratification--meromictic lakes--microbial loop--stratification--trophic scheme--algal bloom--ecosystem modeling--limiting factor--nutrient availability--phytoplankton--saline lake--trophic interaction--russian federation--algae--bacteria (microorganisms)--cyanobacteria--lyngbya--lyngbya contorta
Аннотация: The paper summarises the results of a three-year research study (European Union Grant: INTAS 97-0519) aimed at investigating the planktonic populations and trophic organization of the Lake Shira ecosystem - a saline lake in Khakasia, Siberia. The lake exhibits a stable summer-autumn stratification of the chemical-biological components. The mechanisms responsible for the 'blooming' of phytoplankton in the deeper layers were investigated in greater detail, using data from both field and laboratory experiments. The spectra of nutrition were examined to estimate the relationships between the specific growth rates of the hydrobionts and the influence of the limiting factors: light, nutrients. The observed heterotrophic capability of a metalimnetic phytoplankton population might help explain the development in the deeper waters of Lyngbya contorta. The scheme of trophic interactions was put up, based on the assessment of the carbon pools and carbon flows in the pelagic zone of the lake. A mathematical model of the vertical structure of the lake's plankton populations was constructed, using the ecosystem description and data of vertical turbulent diffusion. The role of light and nutrient limitations and grazing mortality in forming the vertical inhomogeneities, particularly in lowering the depth of the maximal cyanobacterial biomass, has been demonstrated. The theoretical curves for the stratification of chemical and biological parameters have been brought in conformity with the field observations, e.g. for the different patterns of the peaks, and for the biomass maxima of cyanobacteria, purple and green sulphur bacteria, oxygen, and hydrogen sulphide. The calculations revealed that for an adequate assessment of the parameters for the hydrogen sulphide zone it is necessary to introduce flows of allochthonous organic matter. Based on the form of the sulphur distribution curve, the allochthonous input of organic matter and the inflow of hydrogen sulphide from the bottom have been theoretically discriminated for the first time. It has also been ascertained that irrespective of the depth the allochthonous substances limiting bacterial growth, the bacteria are uniformly distributed over depth and can serve as an indicator of the presence of limitation (the effect of autostabilisation in space). Of indisputable interest to limnology are the specific methods developed for understanding the functioning of Lake Shira ecosystem. These include the autostabilisation of the limiting factors, the on-the-spot fluorescent method of determining the three classes of microalgae, the algal mixotrophy and the planktonic population interactions and feedbacks, and development of a more sensitive, bioluminescent method for mapping the nonhomogeneities. Owing to a balanced combination of classical approaches (field observations, in situ data on production-decomposition) and the more recent ones (satellite monitoring, biophysical methods of estimating interactions of populations, mathematical models based on the field and experimental data), many of the structural-function relationships in the ecosystem can now be explained, and the models can provide 'mutual control and mutual agreement' between the data collected using different approaches.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Degermendzhy A.G., Belolipetsky V.M., Zotina T.A., Gulati R.D.
Заглавие : Formation of the vertical heterogeneity in the Lake Shira ecosystem: The biological mechanisms and mathematical model
Место публикации : Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - С. 271-297. - ISSN 13862588 (ISSN) , DOI 10.1023/A:1015621508971
Ключевые слова (''Своб.индексиров.''): hydrogen sulphide--phytoplankton--stratification control--sulphate-reducing bacteria--sulphur cycle--vertical model--biological production--community structure--ecosystem modeling--nutrient cycling--plankton--saline lake--seasonal variation--vertical distribution--russian federation--algae--arctodiaptomus--bacteria (microorganisms)--calanoida--chlorophyta--copepoda--crustacea--cyanobacteria--dictyosphaerium--lyngbya--lyngbya contorta
Аннотация: Data on the seasonal changes in vertical heterogeneity of the physical-chemical and biological parameters of the thermally stratified Shira Lake ecosystem (Khakasia, Siberia) in 1996-2000 have been analyzed. The interaction mechanisms involving: (1) The plankton populations in aerobic and anaerobic zones, involving the cycling of carbon and sulphur, (2) the primary production limitation (by light and phosphorus) and inhibition (by light), and (3) the kinetic characteristics of plankton populations have been elucidated. A mathematical model of the vertical structure of the lake's plankton populations, based on the ecosystem description and on vertical turbulent diffusion of the matter, has been constructed. The green alga Dictyosphaerium tetrachotomum (Chlorophyta) and the cyanobacterium Lyngbya contorta (Cyanophyta), which dominated the phytoplankton biomass, were taken as oxygen producers. Arctodiaptomus salinus (a calanoid copepod) has been assumed as the main grazer in Shira Lake as it dominated the zooplankton biomass. Four groups of microorganisms involved in the sulphur cycle formation have been distinguished: sulphur, sulphur purple, sulphur green and SRB. H2S is oxidized to sulphate (only the green sulphur bacteria oxidize it to sulphur), and sulphate is reduced to H2S, forming neither sulphur nor its water-soluble compounds. The role of grazing, light and nutrient limitation, in forming the vertical inhomogeneities, particularly in lowering the depth of the maximal cyanobacterial biomass, has been demonstrated. When the model takes into account both light limitation and nutrient limitation of algal growth by P and consumption of algae by crustaceans: (a) in the scenario where the P is formed only by the cycling and decomposition of autochthonous organic matter, both the green algae and cyanobacteria are eliminated; (b) in the scenario involving an additional P flux in the deep water layers the peak of the cyanobacteria is at a depth of 10 m, and its amplitude is close to the one observed in the lake. The position of the peak remains stable owing to the 'double' limitation mechanism: light 'from above' and P 'from below'. Another mechanism responsible for the deep position of the peak of cyanobacteria was analyzed mathematically based on the model involving the experimentally proven assumption of the growth inhibition by light in the epilimnion and the light limitation in the hypolimnion. The main result is: the peak is positioned stable at its depth and does not change with time. The analytical and numerical calculations made for this positioning mechanism yielded the formulae relating the depth of the maximum of algal biomass, the 'width' of the peak base and the peak amplitude and a number of parameters (algae elimination, turbulent diffusion coefficient, sedimentation rate, light extinction coefficient and light intensity). The theoretical curves for the stratification of chemical and biological parameters have been brought in conformity with field observations, e.g. for the different patterns for the peaks, and the biomass maxima of cyanobacteria, purple and green sulphur bacteria, oxygen, and hydrogen sulphide. The calculations revealed that for an adequate assessment of the parameters for the hydrogen sulphide zone it is necessary to introduce flows of allochthonous organic matter. For the first time, theoretically, based on the form of the sulphur distribution curve, the allochthonous input of organic matter and the inflow of hydrogen sulphide from the bottom have been discriminated. The theoretical limit for the depth up to which the hydrogen-sulphide zone can ascend under the impact of allochthonous organic loading, has been determined.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kiselev E.G., Shishatskaya E.I., Zhila N.O., Boyandin A.N., Syrvacheva D.A., Vinogradova O.N., Kalacheva G.S., Vasiliev A.D., Peterson I.V.
Заглавие : Cell growth and accumulation of polyhydroxyalkanoates from CO2 and H2 of a hydrogen-oxidizing bacterium, Cupriavidus eutrophus B-10646
Место публикации : Bioresource Technology. - 2013. - Vol. 146. - С. 215-222. - ISSN 09608524 (ISSN) , DOI 10.1016/j.biortech.2013.07.070
Ключевые слова (''Своб.индексиров.''): autotrophic synthesis--cupriavidus eutrophus--polyhydroxyalkanoates
Аннотация: Synthesis of polyhydroxyalkanoates (PHAs) by a new strain of Cupriavidus - Cupriavidus eutrophus B-10646 - was investigated under autotrophic growth conditions. Under chemostat, at the specific flow rate D=0.1h-1, on sole carbon substrate (CO2), with nitrogen, sulfur, phosphorus, potassium, and manganese used as growth limiting elements, the highest poly(3-hydroxybutyrate) [P(3HB)] yields were obtained under nitrogen deficiency. In batch autotrophic culture, in the fermenter with oxygen mass transfer coefficient 0.460h-1, P(3HB) yields reached 85% of dry cell weight (DCW) and DCW reached 50g/l. Concentrations of supplementary PHA precursor substrates (valerate, hexanoate, ?-butyrolactone) and culture conditions were varied to produce, for the first time under autotrophic growth conditions, PHA ter- and tetra-polymers with widely varying major fractions of 3-hydroxybutyrate, 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate monomer units. Investigation of the high-purity PHA specimens showed significant differences in their physicochemical and physicomechanical properties. В© 2013 Elsevier Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Trusova M.Y., Kalacheva G.S., Kozhevnicov I.V.
Заглавие : Physiological-biochemical properties and the ability to synthesize polyhydroxyalkanoates of the glucose-utilizing strain of the hydrogen bacterium Ralstonia eutropha B8562.
Место публикации : Applied microbiology and biotechnology. - 2006. - Vol. 73, Is. 2. - С. 429-433. - ISSN 01757598 (ISSN) , DOI 10.1007/s00253-006-0460-0
Ключевые слова (''Своб.индексиров.''): beta hydroxyvaleric acid--beta-hydroxyvaleric acid--carbon dioxide--ester--fatty acid--fructose--glucose--hexanoic acid derivative--hydrogen--hydroxyacid--oxygen--polymer--ribosome rna--valeric acid derivative--article--biotechnology--chemistry--metabolism--methodology--wautersia eutropha--biotechnology--carbon dioxide--cupriavidus necator--esters--fatty acids--fructose--glucose--hexanoic acids--hydrogen--hydroxy acids--oxygen--pentanoic acids--polymers--rna, ribosomal
Аннотация: Physiological-biochemical, genetic, and cultural properties of the glucose-utilizing mutant strain Ralstonia eutropha B8562 have been compared with those of its parent strain R. eutropha B5786. It has been shown that growth characteristics of the strain cultured on glucose as the sole carbon and energy source are comparable with those of the parent strain. Strain B8562 is characterized by high polyhydroxyalkanoate (PHA) yields on different carbon sources (CO(2), fructose, and glucose). PHA accumulation in the strain batch cultured on glucose under nitrogen deficiency reaches 90 %. The major monomer in the PHA is beta-hydroxybutyric acid (more than 99 mol %); the identified minor components are beta-hydroxyvaleric acid (0.25-0.72 mol %) and beta-hydroxyhexanoic acid (0.08-1.5 mol %). The strain is a promising PHA producer on available sugar-containing media with glucose.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shatilina Z.M., Gubanov M.V., Zadereev E.S., Pavlichenko V.V., Axenov-Gribanov D.V., Sapozhnikova E.A., Protopopova M.V., Bedulina D.S., Timofeyev M.A.
Заглавие : Comparative study of Thermoresistances' cellular mechanisms in representatives of the Gammarus lacustris Sars populations inhabiting Saline Lake Shira (Republic of Khakassia) and a fresh water body in the Cis-Baikal region
Место публикации : Doklady Biological Sciences. - 2010. - Vol. 434, Is. 1. - С. 359-362. - ISSN 00124966 (ISSN) , DOI 10.1134/S0012496610050182
Ключевые слова (''Своб.индексиров.''): antioxidant--catalase--fresh water--heat shock protein 70--reactive oxygen metabolite--sea water--amphipoda--animal--article--biosynthesis--comparative study--ecosystem--heat--metabolism--russian federation--amphipoda--animals--antioxidants--catalase--ecosystem--fresh water--hot temperature--hsp70 heat-shock proteins--reactive oxygen species--seawater--siberia--gammarus--gammarus lacustris
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zadereev E.S., Tolomeyev A.P., Drobotov A.V., Emeliyanova A.Y., Gubanov M.V.
Заглавие : The vertical distribution and abundance of Gammarus lacustris in the pelagic zone of the meromictic lakes Shira and Shunet (Khakassia, Russia)
Место публикации : Aquatic Ecology. - 2010. - Vol. 44, Is. 3. - С. 531-539. - ISSN 13862588 (ISSN) , DOI 10.1007/s10452-010-9329-5
Ключевые слова (''Своб.индексиров.''): amphipod--gammarus lacustris--meromictic lake--thermocline--vertical distribution--abundance--amphipod--dissolved oxygen--intertidal environment--meromictic lake--pelagic environment--seston--stratification--thermocline--vertical distribution--videography--water temperature--khakassia--lake shira--lake shunet--russian federation--amphipoda--animalia--gammarus--gammarus lacustris
Аннотация: The vertical distribution and abundance of Gammarus lacustris in the pelagic zone of two fishless meromictic lakes, L. Shira and L. Shunet, in Southern Siberia (Russia), was studied with the underwater video recording system and using vertical hauls. In both lakes, during summer stratification, Gammarus was distributed non-homogenously, with a stable peak in the metalimnion. The average depth of Gammarus population in the pelagic zone was significantly correlated with the depth of the thermocline. Gammarus abundances obtained using vertical plankton hauls with net were quite comparable with those obtained from video records. The peak abundance of Gammarus in the pelagic zone of the lakes observed with underwater video amounted up to 400 individuals m-2, while the peak animal densities in the metalimnion reached 50 ind. m-3. The data are compared with previously published abundances of Gammarus in the littoral of Lake Shira. Both littoral and pelagic can be equally important habitats for amphipods in meromictic lakes. The absence of fish in the pelagic zone, high oxygen concentration, low water temperature, increased seston concentration, elevated water density in the metalimnion and the anoxic hypolimnion can be the most probable combination of factors that are responsible for the peak of Gammarus in the metalimnion of these lakes. В© 2010 Springer Science+Business Media B.V.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Maillard-Schaller E., Kuettel O.M., Diederich L., Schlapbach L., Zhirnov V.V., Belobrov P.I.
Заглавие : Surface properties of nanodiamond films deposited by electrophoresis on Si(100)
Место публикации : Diamond and Related Materials. - 1999. - Vol. 8, Is. 2-5. - С. 805-808. - ISSN 09259635 (ISSN)
Ключевые слова (''Своб.индексиров.''): energy band diagram--nanodiamond--raman spectroscopy--surface characterization--band structure--electrodeposition--electrophoresis--hydrogen--nanostructured materials--nitrogen--oxidation--oxygen--phonons--plasma applications--silicon wafers--surface properties--dielectrophoresis--negative electron affinity (nea)--phonon confinement effect--diamond films
Аннотация: The surface properties of diamond nanoparticles (40-50 A in diameter) have been investigated by X-ray photoelectron spectroscopy (XPS), UV photoelectron spectroscopy (UPS) and Raman spectroscopy. The diamond nanoparticles have been deposited on flat Si(100) substrates by electrophoresis/dielectrophoresis. The as-deposited films are strongly oxidized and present a 1-2% nitrogen content. After treatment at 850 В°C in H2 plasma for 60 min, the oxygen is removed, and the position of the C 1s core-level peak indicates a n-type electronic comportment of the diamond nanoparticles. Raman spectroscopy of the as-deposited film shows a sp3 contribution at 1321 cm-1 and a sp2 contribution around 1620 cm-1. The 12 cm-1 shift of the sp3 contribution with respect to the bulk diamond peak at 1333 cm-1 is attributed to a phonon confinement effect due to the size of the diamond particles. The H2 plasma treatment induces a size decrease of the nanocrystallites confirmed by Raman and scanning electron microscopy (SEM) measurements. UPS spectroscopy shows a negative electron affinity of -0.2 eV of the hydrogenated nanodiamond film.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ushakova S.A., Tikhomirov A.A., Shikhov V.N., Gros J.-B., Golovko T.K., Dal'Ke I.V., Zakhozhii I.G.
Заглавие : Tolerance of wheat and lettuce plants grown on human mineralized waste to high temperature stress
Место публикации : Advances in Space Research. - 2013. - Vol. 51, Is. 11. - С. 2075-2083. - ISSN 02731177 (ISSN) , DOI 10.1016/j.asr.2013.01.017
Ключевые слова (''Своб.индексиров.''): air temperature step--blss--heat resistance--human waste recycling--lettuce--wheat--air temperature--blss--human waste--lettuce--wheat--atmospheric temperature--electromagnetic field effects--electromagnetic fields--gas plants--heat resistance--oxygen supply--plants (botany)--productivity--specific heat--thermal stress--waste utilization--plant shutdowns
Аннотация: The main objective of a life support system for space missions is to supply a crew with food, water and oxygen, and to eliminate their wastes. The ultimate goal is to achieve the highest degree of closure of the system using controlled processes offering a high level of reliability and flexibility. Enhancement of closure of a biological life support system (BLSS) that includes plants relies on increased regeneration of plant waste, and utilization of solid and liquid human wastes. Clearly, the robustness of a BLSS subjected to stress will be substantially determined by the robustness of the plant components of the phototrophic unit. The aim of the present work was to estimate the heat resistance of two plants (wheat and lettuce) grown on human wastes. Human exometabolites mineralized by hydrogen peroxide in an electromagnetic field were used to make a nutrient solution for the plants. We looked for a possible increase in the heat tolerance of the wheat plants using changes in photosynthetically active radiation (PAR) intensity during heat stress. At age 15 days, plants were subjected to a rise in air temperature (from 23 В± 1 В°C to 44 В± 1 В°) under different PAR intensities for 4 h. The status of the photosynthetic apparatus of the plants was assessed by external 2 gas exchange and fluorescence measurements. The increased irradiance of the plants during the high temperature period demonstrated its protective action for both the photosynthetic apparatus of the leaves and subsequent plant growth and development. The productivity of the plants subjected to temperature changes at 250 W m-2 of PAR did not differ from that of controls, whereas the productivity of the plants subjected to the same heat stress but in darkness was halved. В© 2012 COSPAR. Published by Elsevier Ltd.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tikhomirov A.A., Kudenko Y.A., Degermendzhi A.G., Trifonov S.V., Sutormina E.F., Ivanova Y.A.
Заглавие : Assessment of composition and toxicity for plants of gases produced during physicochemical processing of human exometabolites as applied to biotechnical life support systems
Место публикации : Doklady Biochemistry and Biophysics. - 2011. - Vol. 441, Is. 1. - С. 252-254. - ISSN 16076729 (ISSN) , DOI 10.1134/S1607672911060032
Ключевые слова (''Своб.индексиров.''): ammonia--carbon dioxide--nitrogen oxide--oxygen--article--biomass--bioremediation--drug effect--gas--growth, development and aging--human--instrumentation--methodology--microclimate--plant--waste management--ammonia--biodegradation, environmental--biomass--carbon dioxide--ecological systems, closed--gases--humans--life support systems--nitrogen oxides--oxygen--plants--waste management
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12.

Вид документа : Статья из сборника (выпуск продолж. издания)
Шифр издания :
Автор(ы) : Tikhomirov A.A., Ushakova S.A., Shikhov V.N., Gros J.-B., Golovko T.K., Dal'Ke I.V., Zakhozhii I.G.
Заглавие : Tolerance of plants grown on human mineralized waste to changes in air temperature
Место публикации : International Astronautical Federation - 59th International Astronautical Congress 2008, IAC 2008. - 2008. - Vol. 1: 59th International Astronautical Congress 2008, IAC 2008 (29 September 2008 through 3 October 2008, Glasgow) Conference code: 79748. - С. 311-320
Ключевые слова (''Своб.индексиров.''): air temperature--alternating electromagnetic field--biological life support systems--closedness--controlled process--fluorescence measurements--gas exchange--heat stress--high temperature--human waste--nutrient solution--old plants--photosynthetic apparatus--photosynthetically active radiation--plant components--plant growth--plant wastes--protective action--reproductive organs--temperature changes--temperature conditions--atmospheric temperature--electromagnetic field effects--electromagnetic fields--gas plants--heat resistance--hydrogen peroxide--oxygen supply--plant life extension--productivity--specific heat--thermal stress--waste utilization--plant shutdowns
Аннотация: The main objective of an LSS is to supply a crew with food, water and oxygen, and to eliminate its waste. The ultimate goal is to achieve the highest degree of closure of the system using controlled processes offering a high level of reliability and flexibility. Enhancement of closedness of biological life support systems (BLSS) including plants relies on increased regeneration of gas, water and plant waste, and utilization of solid and liquid human wastes. Clearly, the robustness of an LSS subjected to stress will be substantially determined by the robustness of the plant components of the phototrophic unit. The aim of the present work was to estimate the heat resistance of plants grown on human wastes. Human exometabolites mineralized by hydrogen peroxide in an alternating electromagnetic field were used to make a nutrient solution for the plants. We looked for a possible increase in the heat resistance of the plants using changes in photosynthetically active radiation (PAR) intensity during heat stress. At ages 15 and 25 days, plants were subjected to a rise in air temperature (from 22-24В°C to 44В°C) under different PAR intensities for 4 hours. The status of the photosynthetic apparatus of the plants was assessed by external CO2 gas exchange and fluorescence measurements. The increased irradiance of the plants during the high temperature period demonstrated its protective action for both the photosynthetic apparatus of the leaves and subsequent plant growth and development. The productivity of the plants subjected to temperature changes at 250 W/m2 of PAR did not differ from that of controls, whereas the productivity of the plants subjected to the same heat stress but in darkness was halved. The heat resistance of the reproductive organs of 25-day-old plants was significantly lower than that of 15-day-old plants subjected to similar light and temperature conditions.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tikhomirov A.A., Ushakova S.A., Kovaleva N.P., Lamaze B., Lobo M., Lasseur Ch.
Заглавие : Biological life support systems for a Mars mission planetary base: Problems and prospects
Место публикации : Advances in Space Research. - 2007. - Vol. 40, Is. 11. - С. 1741-1745. - ISSN 02731177 (ISSN) , DOI 10.1016/j.asr.2006.11.009
Ключевые слова (''Своб.индексиров.''): blss--matter turnover--wastes utilization--biological systems--biophysics--martian surface analysis--physical chemistry--problem solving--biological life support systems--mars mission planetary base--matter turnover--wastes utilization--life support systems (spacecraft)
Аннотация: The study develops approaches to designing biological life support systems for the Mars mission - for the flight conditions and for a planetary base - using experience of the Institute of Biophysics of the Siberian Branch of the Russian Academy of Sciences (IBP SB RAS) with the Bios-3 system and ESA's experience with the MELISSA program. Variants of a BLSS based on using Chlorella and/or Spirulina and higher plants for the flight period of the Mars mission are analyzed. It is proposed constructing a BLSS with a closed-loop material cycle for gas and water and for part of human waste. A higher-plant-based BLSS with the mass exchange loop closed to various degrees is proposed for a Mars planetary base. Various versions of BLSS configuration and degree of closure of mass exchange are considered, depending on the duration of the Mars mission, the diet of the crew, and some other conditions. Special consideration is given to problems of reliability and sustainability of material cycling in BLSS, which are related to production of additional oxygen inside the system. Technologies of constructing BLSS of various configurations are proposed and substantiated. Reasons are given for using physicochemical methods in BLSS as secondary tools both during the flight and the stay on Mars. В© 2007.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tikhomirov A.A., Ushakova S.A., Manukovsky N.S., Lisovsky G.M., Kudenko Yu.A., Kovalev V.S., Gubanov V.G., Barkhatov Yu.V., Gribovskaya I.V., Zolotukhin I.G., Gros J.B., Lasseur Ch.
Заглавие : Mass exchange in an experimental new-generation life support system model based on biological regeneration of environment
Место публикации : Advances in Space Research. - 2003. - Vol. 31, Is. 7. - С. 1711-1720. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(03)80017-0
Ключевые слова (''Своб.индексиров.''): biomass--photosynthesis--plants (botany)--transpiration--mass exchange--life support systems (spacecraft)--ammonia--nitrogen--oxygen--biosphere--animal--annelid worm--article--biological model--biomass--bioremediation--evaluation--growth, development and aging--human--metabolism--microclimate--photosynthesis--pleurotus--radish--wheat--ammonia--animals--biodegradation, environmental--biomass--ecological systems, closed--evaluation studies--humans--life support systems--models, biological--nitrogen--oligochaeta--oxygen--photosynthesis--pleurotus--raphanus--triticum
Аннотация: An experimental model of a biological life support system was used to evaluate qualitative and quantitative parameters of the internal mass exchange. The photosynthesizing unit included the higher plant component (wheat and radish), and the heterotrophic unit consisted of a soil-like substrate, California worms, mushrooms and microbial microflora. The gas mass exchange involved evolution of oxygen by the photosynthesizing component and its uptake by the heterotroph component along with the formation and maintaining of the SLS structure, growth of mushrooms and California worms, human respiration, and some other processes. Human presence in the system in the form of "virtual human" that at regular intervals took part in the respirative gas exchange during the experiment. Experimental data demonstrated good oxygen/carbon dioxide balance, and the closure of the cycles of these gases was almost complete. The water cycle was nearly 100% closed. The main components in the water mass exchange were transpiration water and the watering solution with mineral elements. Human consumption of the edible plant biomass (grains and roots) was simulated by processing these products by a unique physicochemical method of oxidizing them to inorganic mineral compounds, which were then returned into the system and fully assimilated by the plants. The oxidation was achieved by "wet combustion" of organic biomass, using hydrogen peroxide following a special procedure, which does not require high temperature and pressure. Hydrogen peroxide is produced from the water inside the system. The closure of the cycle was estimated for individual elements and compounds. Stoichiometric proportions are given for the main components included in the experimental model of the system. Approaches to the mathematical modeling of the cycling processes are discussed, using the data of the experimental model. Nitrogen, as a representative of biogenic elements, shows an almost 100% closure of the cycle inside the system. The proposed experimental model of a biological system is discussed as a candidate for potential application in the investigations aimed at creating ecosystems with largely closed cycles of the internal mass exchange. The formation and maintenance of sustainable cycling of vitally important chemical elements and compounds in biological life support systems (BLSS) is an extremely pressing problem. To attain the stable functioning of biological life support systems (BLSS) and to maintain a high degree of closure of material cycles in them, it is essential to understand the character of mass exchange processes and stoichiometric proportions of the initial and synthesized components of the system. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gros J.B., Poughon L., Lasseur C., Tikhomirov A.A.
Заглавие : Recycling efficiencies of C,H,O,N,S, and P elements in a biological life support system based on micro-organisms and higher plants
Место публикации : Advances in Space Research. - 2003. - Vol. 31, Is. 1. - С. 195-199. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(02)00739-1
Ключевые слова (''Своб.индексиров.''): biomass--biosynthesis--chemical elements--earth atmosphere--ecosystems--life support systems (spacecraft)--manned space flight--microorganisms--plants (botany)--wastes--compartments--space research--carbon--carbon dioxide--hydrogen--nitrogen--oxygen--phosphorus--sulfur--space technology--article--biological model--biomass--chemistry--comparative study--computer simulation--growth, development and aging--metabolism--methodology--microbiology--microclimate--plant--waste management--biomass--carbon--carbon dioxide--computer simulation--ecological systems, closed--environmental microbiology--hydrogen--life support systems--models, biological--nitrogen--oxygen--phosphorus--plants, edible--sulfur--waste management
Аннотация: MELiSSA is a microorganism based artificial ecosystem conceived as a tool for understanding the behavior of ecosystems and developing the technology for future Manned Space Missions. MELiSSA is composed of four compartments colonized by the microorganisms required by the function of this ecosystem : breakdown of waste produced by men, regeneration of atmosphere and biosynthesis of edible biomass. This paper reports the mass balance description of a Biological Life Support System composed of the MELiSSA loop and of a Higher Plant Compartment working in parallel with the photosynthetic Spirulina compartment producing edible biomass. The recycling efficiencies of the system are determined and compared for various working conditions of the MELiSSA loop with or without the HPC. В© 2002 Published by Elsevier Science Ltd on behalf of COSPAR.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gitelson J.I., Okladnikov Yu.N.
Заглавие : Consistency of gas exchange of man and plants in a closed ecological system: Lines of attack on the problem
Место публикации : Advances in Space Research. - 1996. - Vol. 18, Is. 1-2. - С. 205-210. - ISSN 02731177 (ISSN)
Ключевые слова (''Своб.индексиров.''): carbon dioxide--oxygen--article--atmosphere--biological model--breathing--human--metabolism--microclimate--nutrition--photosynthesis--physiology--plant--plant physiology--atmosphere--carbon dioxide--ecological systems, closed--humans--models, biological--nutrition physiology--oxygen--photosynthesis--plant physiology--plants--respiration
Аннотация: Gas exchange between man and plants in a closed ecological system based on atmosphere regeneration by plant photosynthesis is made consistent by attaining the equilibrium of human CO2 discharge and the productivity of the gas consuming bioregenerator. In this case the gas exchange might be, however, qualitatively disturbed from the equilibrium in terms of oxygen making it accumulate or decrease continuously in the air of the system. Gas exchange equilibrium in terms of O2 was attained in long-term experiments by equality of the human respiration coefficient and the plant assimilation coefficient. Varying the ratio of these parameters it is possible to control the oxygen concentration in the atmosphere to be reclaimed.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gitelson J.I. U - Okladnikov YuN
Заглавие : Man as a component of a closed ecological life support system
Место публикации : Life support & biosphere science : international journal of earth space. - 1994. - Vol. 1, Is. 2. - С. 73-81. - ISSN 10699422 (ISSN)
Ключевые слова (''Своб.индексиров.''): carbon dioxide--oxygen--article--blood analysis--blood cell count--breathing--construction work and architectural phenomena--crop--diet--growth, development and aging--human--metabolism--microclimate--physiology--russian federation--space flight--standard--waste management--water supply--blood cell count--blood chemical analysis--carbon dioxide--crops, agricultural--diet--ecological systems, closed--environment, controlled--facility design and construction--humans--life support systems--oxygen--respiration--russia--space flight--waste management--water supply
Аннотация: Material support of all manned space flights so far has been provided from a prestored stock of substances or replenished from the Earth's biosphere. Exploration of space will, however, become real only when man is able to break away from Earth completely, when he will be accompanied by a system providing everything necessary to sustain full-valued life for an unlimited time. The only known system to date meeting this requirement is the Earth's biosphere. To break away from his cradle, as K.E. Tsiolkovsky called Earth, it is necessary to devise a life support system functionally similar to the natural biosphere. This need not be similar in structure to the vast diversity of trophic relationships available on Earth, but requires the solution of a multitude of various problems of an ecological, physiological, engineering and social-psychological nature. Human life-support systems based on biological regeneration of environments in small volumes have been studied at the Institute of Biophysics (Siberian Branch of the Russian Academy of Sciences) over many years. This work has resulted in the design of Bios-3, a biologically-based self-sustained human life support system.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vysotski E.S., Trofimov K.P., Bondar' V.S., Gitelson J.I.
Заглавие : Luminescence of Ca(2+)-activated photoprotein obelin initiated by NaOCl and MnCl2.
Место публикации : Journal of bioluminescence and chemiluminescence. - 1993. - Vol. 8, Is. 6. - С. 301-305. - ISSN 08843996 (ISSN)
Ключевые слова (''Своб.индексиров.''): calcium--chloride--hypochlorite sodium--manganese chloride--manganese derivative--obelin--photoprotein--article--chemistry--drug effect--kinetics--luminescence--metabolism--calcium--chlorides--kinetics--luminescence--luminescent proteins--manganese compounds--sodium hypochlorite
Аннотация: The luminescence of obelin is initiated by NaOCl in a reaction mixture containing no calcium. The addition of Mn2+ enhances the light emission 300-fold. Sodium azide and histidine, as singlet oxygen quenchers, inhibit NaOCl-activated obelin luminescence in the presence or absence of Mn2+. This suggests that the addition of NaOCl to the mixture causes singlet oxygen formation (stimulated by Mn2+ ions), and singlet oxygen initiates the light-emitting reaction.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sarangova A.B., Somova L.A., Pechurkin N.S.
Заглавие : On monitoring the bacterial component as an indicator of the state of small man-made ecosystems
Место публикации : Advances in Space Research. - 2001. - Vol. 27, Is. 9. - С. 1605-1609. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(01)00256-3
Ключевые слова (''Своб.индексиров.''): bacteria--ecosystems--substrates--intracellular substrate concentration--space research--catalase--oxidoreductase--artificial ecosystem--article--bacterial phenomena and functions--biomass--culture medium--ecosystem--enzymology--growth, development and aging--metabolism--microbiology--oxygen consumption--pseudomonas--bacterial physiology--biomass--catalase--culture media--ecosystem--oxidoreductases--oxygen consumption--pseudomonas--water microbiology
Аннотация: High reproduction rates make the bacterial component of ecosystems a good indicator of the state of the system on the whole. This determines the necessity to develop rapid monitoring of the functional state of the bacterial component of small ecosystems. Information about substrate concentration in the population is indicative of the state of the bacterial culture. Conventional methods of monitoring the concentration of integral substrate in the system take time much longer than the changes in the ecosystem. The paper presents theoretical foundations for the logical sequence "catalase activity - intracellular substrate concentration - estimate of substrate consumed by bacteria" for experimental verification and as a consequence of development of the integral method of monitoring the bacterial population on the basis of determining bacterial catalase activity. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pisman T.I., Pechurkin N.S., Babkin A.V., Somova L.A., Sarangova A.B.
Заглавие : Experimental and mathematical models for small aqueous closed ecosystems with spatially separated components
Место публикации : Advances in Space Research. - 1999. - Vol. 24, Is. 3. - С. 361-366. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(99)00486-X
Ключевые слова (''Своб.индексиров.''): carbon dioxide--nitrogen--oxygen--quaternary ammonium derivative--aquatic environment--artificial ecosystem--ecological modeling--trophic interaction--animal--article--biological model--candida--chlorella--fermentation--mathematics--metabolism--microclimate--paramecium--photosynthesis--animals--candida--carbon dioxide--chlorella--ecological systems, closed--fermentation--mathematics--models, biological--nitrogen--oxygen--paramecium--photosynthesis--quaternary ammonium compounds
Аннотация: Experimental and theoretical models of closed 'autotroph-heteretroph' (chlorella-yeast, chlorella- protozoa) ecosystems with spatially separated components have been created and studied. The chart of flows and interaction of components of gas-closed 'chlorella-yeast' system have formed the basis describe mathematically the functioning of the given system, experimental results have been found to agree with computer solution of the problem in terms of quality. Investigation of the experimental model of the 'producer-consumer' trophic chain demonstrated the role of protozoa in nitrogen turnover. 'Production-decomposition' and 'production-grazing-decomposition' cycle models has been theoretically analyzed and compared. The predator has been shown to be a more intensive mineralizer than the reducer component.
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