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Production and Properties of Microbial Polyhydroxyalkanoates Synthesized from Hydrolysates of Jerusalem Artichoke Tubers and Vegetative Biomass / T. G. Volova, E. G. Kiselev, A. V. Demidenko [et al.] // Polymers. - 2022. - Vol. 14, Is. 1. - Ст. 132, DOI 10.3390/polym14010132. - Cited References:93. - This study was financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No. 075-15-2021-626) in accordance with Resolution No. 220 of the Government of the Russian Federation of 9 April 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning" (polymer synthesis, properties), and by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (films production, surface properties). . - ISSN 2073-4360

РУБ Polymer Science
Рубрики:
GLUCOSE-UTILIZING STRAIN
   RALSTONIA-EUTROPHA
   ASPERGILLUS-NIGER
   ACID
Кл.слова (ненормированные):
Jerusalem artichoke hydrolysates -- PHA synthesis -- productivity -- polyhydroxyalkanoates
Аннотация: One of the major challenges in PHA biotechnology is optimization of biotechnological processes of the entire synthesis, mainly by using new inexpensive carbon substrates. A promising substrate for PHA synthesis may be the sugars extracted from the Jerusalem artichoke. In the present study, hydrolysates of Jerusalem artichoke (JA) tubers and vegetative biomass were produced and used as carbon substrate for PHA synthesis. The hydrolysis procedure (the combination of aqueous extraction and acid hydrolysis, process temperature and duration) influenced the content of reducing substances (RS), monosaccharide contents, and the fructose/glucose ratio. All types of hydrolysates tested as substrates for cultivation of three strains-C. necator B-10646 and R. eutropha B 5786 and B 8562-were suitable for PHA synthesis, producing different biomass concentrations and polymer contents. The most productive process, conducted in 12-L fermenters, was achieved on hydrolysates of JA tubers (X = 66.9 g/L, 82% PHA) and vegetative biomass (55.1 g/L and 62% PHA) produced by aqueous extraction of sugars at 80 degrees C followed by acid hydrolysis at 60 degrees C, using the most productive strain, C. necator B-10646. The effects of JA hydrolysates on physicochemical properties of PHAs were studied for the first time. P(3HB) specimens synthesized from the JA hydrolysates, regardless of the source (tubers or vegetative biomass), hydrolysis conditions, and PHA producing strain employed, exhibited the 100-120 degrees C difference between the T-melt and T-degr, prevailing of the crystalline phase over the amorphous one (C-x between 69 and 75%), and variations in weight average molecular weight (409-480) kDa. Supplementation of the culture medium of C. necator B-10646 grown on JA hydrolysates with potassium valerate and epsilon-caprolactone resulted in the synthesis of P(3HB-co-3HV) and P(3HB-co-4HB) copolymers that had decreased degrees of crystallinity and molecular weights, which influenced the porosity and surface roughness of polymer films prepared from them. The study shows that JA hydrolysates used as carbon source enabled productive synthesis of PHAs, comparable to synthesis from pure sugars. The next step is to scale up PHA synthesis from JA hydrolysates and conduct the feasibility study. The present study contributes to the solution of the critical problem of PHA biotechnology-finding widely available and inexpensive substrates.

WOS
Держатели документа:
Siberian Fed Univ, Sch Fundamental Biol & Biotechnol, Basic Dept Biotechnol, Krasnoyarsk 660041, Russia.
Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys, SB RAS, Krasnoyarsk 660036, Russia.
Krasnoyarsk Sci Ctr SB RAS, LV Kirensky Phys Inst, Fed Res Ctr, SB RAS, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Fed Res Ctr, Krasnoyarsk Sci Ctr, Siberian Branch, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Volova, Tatiana G.; Kiselev, Evgeniy G.; Demidenko, Alexey V.; Zhila, Natalia O.; Nemtsev, Ivan V.; Lukyanenko, Anna V.; Kiselev, Evgeniy; Project "Agropreparations of the new generation: a strategy of construction and realization" [075-15-2021-626]; State Assignment of the Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]

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Development of Biodegradable Delivery Systems Containing Novel 1,2,4-Trioxolane Based on Bacterial Polyhydroxyalkanoates / S. Y. Lipaikin, I. A. Yaremenko, A. O. Terent'ev [et al.] // Adv. Polym. Technol. - 2022. - Vol. 2022. - Ст. 6353909, DOI 10.1155/2022/6353909. - Cited References:70. - \his study was financially supported by the project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 074-02-2018-328) in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning." . - ISSN 0730-6679. - ISSN 1098-2329

РУБ Engineering, Chemical + Polymer Science
Рубрики:
IN-VITRO
   BRIDGED 1,2,4,5-TETRAOXANES
   ANTIMALARIAL ACTIVITY
   RELEASE
Аннотация: In this work, delivery systems in the form of microparticles and films containing 1,2,4-trioxolane (ozonide, OZ) based on polyhydroxyalkanoates (PHAs) were developed. Main systems' characteristics were investigated: the particle yield, average diameter, zeta potential, surface morphology, loading capacity, and drug release profile of microparticles, as well as surface morphology and release profiles of OZ-containing films. PHA-based OZ-loaded microparticles have been found to have satisfactory size, zeta potential, and ozonide loading-release behavior. It was noted that OZ content influenced the surface morphology of obtained systems.

WOS
Держатели документа:
Siberian Fed Univ, 79 Svobodnyi Av, Krasnoyarsk 660041, Russia.
Russian Acad Sci, ND Zelinsky Inst Organ Chem, 47 Leninsky Prosp, Moscow 119991, Russia.
Inst Biophys SB RAS, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Lipaikin, Sergei Y.; Yaremenko, Ivan A.; Terent'ev, Alexander O.; Volova, Tatiana G.; Shishatskaya, Ekaterina, I; Lipaikin, Sergei; Government of the Russian Federation [074-02-2018-328]

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Properties of Degradable Polyhydroxyalkanoates (PHAs) Synthesized by a New Strain, Cupriavidus necator IBP/SFU-1, from Various Carbon Sources / N. O. Zhila, K. Y. Sapozhnikova, E. G. Kiselev [et al.] // Polymers. - 2021. - Vol. 13, Is. 18. - Ст. 3142, DOI 10.3390/polym13183142. - Cited References:78. - This work was financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 075-15-2021-626) in accordance with Resolution No 220 of the Government of the Russian Federation of 9 April 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning" (strain isolation, polymer synthesis and investigation), and by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (study of film properties). . - ISSN 2073-4360

РУБ Polymer Science
Рубрики:
RALSTONIA-EUTROPHA
   POLY(3-HYDROXYBUTYRATE) PRODUCTION
   PLANT OIL
   ACID
Кл.слова (ненормированные):
Cupriavidus necator IBP -- SFU-1 -- cell growth and PHA synthesis -- various -- carbon sources -- PHA composition and properties -- polymer films
Аннотация: The bacterial strain isolated from soil was identified as Cupriavidus necator IBP/SFU-1 and investigated as a PHA producer. The strain was found to be able to grow and synthesize PHAs under autotrophic conditions and showed a broad organotrophic potential towards different carbon sources: sugars, glycerol, fatty acids, and plant oils. The highest cell concentrations (7-8 g/L) and PHA contents were produced from oleic acid (78%), fructose, glucose, and palm oil (over 80%). The type of the carbon source influenced the PHA chemical composition and properties: when grown on oleic acid, the strain synthesized the P(3HB-co-3HV) copolymer; on plant oils, the P(3HB-co-3HV-co-3HHx) terpolymer, and on the other substrates, the P(3HB) homopolymer. The type of the carbon source influenced molecular-weight properties of PHAs: P(3HB) synthesized under autotrophic growth conditions, from CO2, had the highest number-average (290 +/- 15 kDa) and weight-average (850 +/- 25 kDa) molecular weights and the lowest polydispersity (2.9 +/- 0.2); polymers synthesized from organic carbon sources showed increased polydispersity and reduced molecular weight. The carbon source was not found to affect the degree of crystallinity and thermal properties of the PHAs. The type of the carbon source determined not only PHA composition and molecular weight but also surface microstructure and porosity of the polymer films. The new strain can be recommended as a promising P(3HB) producer from palm oil, oleic acid, and sugars (fructose and glucose) and as a producer of P(3HB-co-3HV) from oleic acid and P(3HB-co-3HV-co-3HHx) from palm oil.

WOS
Держатели документа:
Siberian Fed Univ, Basic Dept Biotechnol, Sch Fundamental Biol & Biotechnol, 79 Svobodnyi Av, Krasnoyarsk 660041, Russia.
SB RAS, Inst Biophys, Fed Res Ctr, Krasnoyarsk Sci Ctr, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
SB RAS, LV Kirensky Inst Phys, Krasnoyarsk Sci Ctr, Fed Res Ctr, 50-38 Akademgorodok, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Fed Res Ctr, Krasnoyarsk Sci Ctr, Siberian Branch, 50 Akademgorodok, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Zhila, Natalia O.; Sapozhnikova, Kristina Yu; Kiselev, Evgeniy G.; Vasiliev, Alexander D.; Nemtsev, Ivan, V; Shishatskaya, Ekaterina, I; Volova, Tatiana G.; Russian FederationRussian Federation [075-15-2021-626, 220]; State Assignment of the Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]

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Properties of degradable polyhydroxyalkanoates (Phas) synthesized by a new strain, cupriavidus necator ibp/sfu-1, from various carbon sources / N. O. Zhila, K. Yu. Sapozhnikova, E. G. Kiselev [et al.] // Polym. - 2021. - Vol. 13, Is. 18. - Ст. 3142, DOI 10.3390/polym13183142 . - ISSN 2073-4360
Кл.слова (ненормированные):
Cell growth and PHA synthesis -- Cupriavidus necator IBP/SFU-1 -- PHA composition and properties -- Polymer films -- Various carbon sources -- Biodegradable polymers -- Carbon -- Carbon films -- Cell proliferation -- Crystallinity -- Fructose -- Glucose -- Long Term Evolution (LTE) -- Oleic acid -- Organic carbon -- Palm oil -- Polydispersity -- Semiconducting films -- Autotrophics -- Carbon source -- Cell growth and PHA synthesis -- Cupriavidu necator IBP/SFU-1 -- PHA composition and property -- Plant oil -- Polyhydroxyalkanoates -- Property -- Synthesised -- Various carbon source -- Polymer films
Аннотация: The bacterial strain isolated from soil was identified as Cupriavidus necator IBP/SFU-1 and investigated as a PHA producer. The strain was found to be able to grow and synthesize PHAs under autotrophic conditions and showed a broad organotrophic potential towards different carbon sources: sugars, glycerol, fatty acids, and plant oils. The highest cell concentrations (7–8 g/L) and PHA contents were produced from oleic acid (78%), fructose, glucose, and palm oil (over 80%). The type of the carbon source influenced the PHA chemical composition and properties: when grown on oleic acid, the strain synthesized the P(3HB-co-3HV) copolymer; on plant oils, the P(3HB-co-3HV-co-3HHx) terpolymer, and on the other substrates, the P(3HB) homopolymer. The type of the carbon source influenced molecular-weight properties of PHAs: P(3HB) synthesized under autotrophic growth conditions, from CO2, had the highest number-average (290 ± 15 kDa) and weight-average (850 ± 25 kDa) molecular weights and the lowest polydispersity (2.9 ± 0.2); polymers synthesized from organic carbon sources showed increased polydispersity and reduced molecular weight. The carbon source was not found to affect the degree of crystallinity and thermal properties of the PHAs. The type of the carbon source determined not only PHA composition and molecular weight but also surface microstructure and porosity of the polymer films. The new strain can be recommended as a promising P(3HB) producer from palm oil, oleic acid, and sugars (fructose and glucose) and as a producer of P(3HB-co-3HV) from oleic acid and P(3HB-co-3HV-co-3HHx) from palm oil. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Scopus
Держатели документа:
Basic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk, 660041, Russian Federation
Federal Research Center, “Krasnoyarsk Science Center SB RAS”, Institute of Biophysics SB RAS, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Research Center, “Krasnoyarsk Science Center SB RAS”, L.V. Kirensky Institute of Physics SB RAS, 50/38 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Research Center, “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences”, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Zhila, N. O.; Sapozhnikova, K. Yu.; Kiselev, E. G.; Vasiliev, A. D.; Nemtsev, I. V.; Shishatskaya, E. I.; Volova, T. G.

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A study of the properties and efficacy of microparticles based on P(3HB) and P(3HB/3HV) loaded with herbicides / R. Vijayamma, H. J. Maria, S. Thomas [et al.] // J. Appl. Polym. Sci. - 2021. - Ст. e51756, DOI 10.1002/app.51756. - Cited References:57. - This work was supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 074-02-2018-328) in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning". Instruments of Krasnoyarsk Regional Center of Research Equipment of Federal Research Center Krasnoyarsk Science Center SB RAS were used. . - Article in press. - ISSN 0021-8995. - ISSN 1097-4628

РУБ Polymer Science
Рубрики:
FENOXAPROP-P-ETHYL
CONTROLLED-RELEASE
BIODEGRADABLE
Кл.слова (ненормированные):
biodegradable -- drug delivery systems -- microparticles
Аннотация: The wide use of pesticides in agriculture has caused uncontrolled distribution of these chemicals in the environment, calling for the development and investigation of new environmentally friendly formulations, which would reduce human impact on nature. In the present study, the metribuzin (MET), tribenuron-methyl (TBM), and fenoxaprop-P-ethyl (FPE) herbicides were encapsulated in microparticles of degradable microbial polymers - polyhydroxyalkanoates (PHAs) - of two types - poly-3-hydroxybutyrate [P(3HB)] and poly(3-hydroxybutyrate-co-3-hydroxyvalerate [P(3HB/3HV)]. The use of P(3HB) resulted in higher yields of microparticles (63% to 79%) and larger sizes of the particles, whose average diameter was 0.60 +/- 0.06-0.75 +/- 0.11 mu m, while the average diameter of copolymer particles varied between 0.43 +/- 0.12 and 0.55 +/- 0.05 mu m. Encapsulation efficiency was rather determined by the type of herbicide and its solubility, varying from 24.7% to 48.2%. In vitro herbicide release from microparticles to water was affected by herbicide solubility and PHA chemical composition. The readily soluble MET showed the highest release rate, and over 30 days, 64% and 78% of the encapsulated amounts were released from P(3HB) and P(3HB/3HV) microparticles, respectively. High herbicidal activity of microparticles loaded with metribuzin and tribenuron-methyl was demonstrated in the laboratory stands of the Elsholtzia ciliata weed plant.

WOS
Держатели документа:
Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Krasnoyarsk, Russia.
Mahatma Gandhi Univ, Int & Inter Univ Ctr Nanosci & Nanotechnol, Kottayam, Kerala, India.
RAS, Krasnoyarsk Sci Ctr SB, Fed Res Ctr, Inst Biophys SB, Krasnoyarsk, Russia.
RAS, Krasnoyarsk Sci Ctr SB, Res Equipment Fed Res Ctr, Krasnoyarsk Reg Ctr, Krasnoyarsk, Russia.
RAS, Krasnoyarsk Sci Ctr SB, Fed Res Ctr, LV Kirensky Inst Phys, Krasnoyarsk, Russia.
Reshetnev Siberian State Univ Sci & Technol, Sci Lab, Krasnoyarsk, Russia.

Доп.точки доступа:
Vijayamma, Raji; Maria, Hanna J.; Thomas, Sabu; Shishatskaya, Ekaterina I.; Kiselev, Evgeniy G.; Nemtsev, Ivan V.; Sukhanova, Anna A.; Volova, Tatiana G.; Project "Agropreparations of the new generation: a strategy of construction and realization" [074-02-2018-328]; Government of the Russian Federation [220]

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A study of the properties and efficacy of microparticles based on P(3HB) and P(3HB/3HV) loaded with herbicides / R. Vijayamma, H. J. Maria, S. Thomas [et al.] // J. Appl. Polym. Sci. - 2021, DOI 10.1002/app.51756 . - Article in press. - ISSN 0021-8995
Кл.слова (ненормированные):
biodegradable -- drug delivery systems -- microparticles -- Agricultural robots -- Controlled drug delivery -- Solubility -- Targeted drug delivery -- Weed control -- 3-Hydroxybutyrate -- Average diameter -- Biodegradable -- Drug-delivery systems -- Human impact -- Metribuzin -- Micro particles -- Poly-3-hydroxybutyrate -- Polyhydroxyalkanoates -- Property -- Herbicides
Аннотация: The wide use of pesticides in agriculture has caused uncontrolled distribution of these chemicals in the environment, calling for the development and investigation of new environmentally friendly formulations, which would reduce human impact on nature. In the present study, the metribuzin (MET), tribenuron-methyl (TBM), and fenoxaprop-P-ethyl (FPE) herbicides were encapsulated in microparticles of degradable microbial polymers – polyhydroxyalkanoates (PHAs) – of two types – poly-3-hydroxybutyrate [P(3HB)] and poly(3-hydroxybutyrate-co-3-hydroxyvalerate [P(3HB/3HV)]. The use of P(3HB) resulted in higher yields of microparticles (63% to 79%) and larger sizes of the particles, whose average diameter was 0.60 ± 0.06–0.75 ± 0.11 ?m, while the average diameter of copolymer particles varied between 0.43 ± 0.12 and 0.55 ± 0.05 ?m. Encapsulation efficiency was rather determined by the type of herbicide and its solubility, varying from 24.7% to 48.2%. In vitro herbicide release from microparticles to water was affected by herbicide solubility and PHA chemical composition. The readily soluble MET showed the highest release rate, and over 30 days, 64% and 78% of the encapsulated amounts were released from P(3HB) and P(3HB/3HV) microparticles, respectively. High herbicidal activity of microparticles loaded with metribuzin and tribenuron-methyl was demonstrated in the laboratory stands of the Elsholtzia ciliata weed plant. © 2021 Wiley Periodicals LLC.

Scopus
Держатели документа:
Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation
International and Inter University Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam, India
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
Krasnoyarsk Regional Center of Research Equipment of Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
L.V. Kirensky Institute of Physics, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
Scientific Laboratory, Reshetnev Siberian State University of Science and Technology, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Vijayamma, R.; Maria, H. J.; Thomas, S.; Shishatskaya, E. I.; Kiselev, E. G.; Nemtsev, I. V.; Sukhanova, A. A.; Volova, T. G.

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Properties of degradable polyhydroxyalkanoates with different monomer compositions / T. Volova, E. Kiselev, I. Nemtsev [et al.] // Int. J. Biol. Macromol. - 2021. - Vol. 182. - P98-114, DOI 10.1016/j.ijbiomac.2021.04.008 . - ISSN 0141-8130
Кл.слова (ненормированные):
Chemical composition -- Films -- Microstructure -- Physicochemical properties -- Polyhydroxyalkanoates -- Surface properties -- 3 hydroxybutyric acid -- 3 hydroxyhexanoate -- 3 hydroxyvalerate -- 4 hydroxybutyric acid -- monomer -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- polymer -- unclassified drug -- Article -- chemical composition -- comparative study -- crystallization -- degradation -- dispersity -- elasticity -- glass transition temperature -- hydrophilicity -- melting point -- molecular weight -- surface property -- synthesis -- thermoregulation -- thermostability
Аннотация: Purpose: To synthesize and investigate polyhydroxyalkanoates (PHAs) with different monomer composition and percentages and polymer films prepared from them. Results: Various PHAs: homopolymer poly-3-hydroxybutyrate P(3HB) and 2-, 3-, and 4-component copolymers comprising various combinations of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), 4-hydroxybutyrate (4HB), and 3-hydroxyhexanoate (3HHx) monomers were synthesized under specialized conditions. Relationships were found between the monomer composition of PHAs and their molecular-weight and thermal properties and degree of crystallinity. All copolymers had decreased weight average molecular weights, Mw (to 390–600 kDa), and increased values of polydispersity (3.2–4.6) compared to the P(3HB). PHA copolymers showed different thermal behavior: an insignificant decrease in Tmelt and the presence of the second peak in the melting region and changes in parameters of crystallization and glass transition. At the same time, they retained thermostability, and the difference between Tmelt and Tdegr was at least 100–120 °C. Incorporation of 4HB, 3HV, and 3HHx monomer units into the 3-hydroxybutyrate chain caused changes in the amorphous to crystalline ratio and decreased the degree of crystallinity (Cx) to 20–40%. According to the degree to which the monomers reduced crystallinity, they were ranked as follows: 4HB – 3HHx – 3HV. A unique set of films was produced; their surface properties and physical/mechanical properties were studied as dependent on PHA composition; monomers other than 3-hydroxybutyrate were found to enhance hydrophilicity, surface development, and elasticity of polymer films. Conclusion: An innovative set of PHA copolymers was synthesized and solution-cast films were prepared from them; the copolymers and films were investigated as dependent on polymer chemical composition. Results obtained in the present study contribute to the solution of a critical issue of producing degradable polymer materials. © 2021 Elsevier B.V.

Scopus
Держатели документа:
Siberian Federal University, 79 Svobodnyi av., Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
L.V. Kirensky Institute of Physics, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/12 Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Volova, T.; Kiselev, E.; Nemtsev, I.; Lukyanenko, А.; Sukovatyi, A.; Kuzmin, A.; Ryltseva, G.; Shishatskaya, E.

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Synthesis and characterization of multicomponent PHAs / E. G. Kiselev, A. D. Vasiliev, T. G. Volova // J. Sib. Fed. Univ. - Biol. - 2021. - Vol. 14, Is. 1. - С. 97-113, DOI 10.17516/1997-1389-0325. - Cited By :1 . - ISSN 1997-1389
Кл.слова (ненормированные):
Biosynthesis -- Copolymers -- Physicochemical properties -- Polyhydroxyalkanoates (PHAs) -- Precursor substrates
Аннотация: Cupriavidus necator B10646 bacterial cells were cultivated in the mode of synthesis of the reserve polyhydroxyalkanoates (PHAs) in the growth medium that contained, in addition to glucose as the main substrate, precursor substrates of the monomers of various monocarboxylic acids - salts of valeric and hexanoic acids, propionate, and ?-butyrolactone. PHA terpolymers and quaterpolymers with different compositions and proportions of monomers were synthesized, and their physicochemical properties were studied. The terpolymers were composed of monomers of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), 4-hydroxybutyrate (4HB), or 3-hydroxyhexanoate (3HHx) and had the following compositions: P(3HB/3HB/4HHx) and P(3HB/3HV/4HHx). The quaterpolymers had the following composition: P(3HB/3HV/4HB/3HHx). All copolymer samples, regardless of the composition and proportions of monomers, had lower molecular weights and higher polydispersity values compared to the highly crystalline 3-hydroxybutyrate homopolymer, but retained thermal stability properties, with a difference between the melting point and thermal degradation of at least 100-110 °C. The inclusion of 3HV, 4HB, and 3HHx monomers in the C-chain of 3HB caused changes in the crystalline to amorphous phase ratio and a significant decrease in the degree of crystallinity (Cx), which depended on the type of monomers and their contents in the copolymer. The maximum decrease in Cx (9-17 %) was detected in the P(3HB/3HV/4HB) terpolymer and the P(3HB/3HV/4HB/3HHx) quaterpolymer (30-36 %). The study confirms that there is the possibility of synthesizing polymers with various compositions, including new ones, which differ significantly in their basic properties. © Siberian Federal University. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, FRC Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
L.V. Kirenskii Institute of Physics SB RAS, FRC Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Kiselev, E. G.; Vasiliev, A. D.; Volova, T. G.

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Biodegradable polymers - Perspectives and applications in agriculture / E. G. Kiselev, N. O. Zhila, T. G. Volova // IOP Conference Series: Earth and Environmental Science : IOP Publishing Ltd, 2021. - Vol. 689: 2020 International Conference on Germany and Russia: Ecosystems Without Borders, EcoSystConfKlgtu 2020 (5 October 2020 through 10 October 2020, ) Conference code: 167944, Is. 1. - Ст. 012036, DOI 10.1088/1755-1315/689/1/012036
Кл.слова (ненормированные):
Biodegradable polymers -- Ecosystems -- Fungi -- Glycerol -- Monounsaturated fatty acids -- Oilseeds -- Pesticides -- Substrates -- Sunflower oil -- Fenoxaprop-p-ethyl -- Natural materials -- Pesticide formulations -- Poly-3-hydroxybutyrate -- Polyhydroxyalkanoates -- Productive process -- Strategy of constructions -- Various substrates -- Palm oil
Аннотация: The paper presents a brief overview of the results of the implementation of the project "Agropreparations of the new generation: a strategy of construction and realization". The first part contains the analysis of the growth of the wild-type strain Cupriavidus necator B-10646 (formerly eutrophus) and the synthesis of polyhydroxyalkanoates by this strain on various substrates: glycerol, palm oil, Siberian oil seed, sunflower seed oils, and oleic acid. On refined glycerin, a highly productive process is implemented when scaling up, allowing to obtain 128 ± 11 g / L PHA. Evaluation of oils has shown that palm oil is the best carbon substrate. The second part presents the results of the development of environmentally friendly slow-release pesticide formulations. They are a degradable matrix of poly-3-hydroxybutyrate mixed with natural materials (peat, clay, wood flour), into which a pesticide (metribuzin, tribenuron-methyl, fenoxaprop-P-ethyl, azoxystrobin, epoxiconazole, and tebuconazole) has been. The developed preparations showed high activity against pathogenic fungi and weeds and had a much weaker negative effect on the soil microflora. Studies of the degradation of the developed preparations and the release of pesticides into the soil confirm their effectiveness over a long period of time, up to 90 days. © Published under licence by IOP Publishing Ltd.

Scopus
Держатели документа:
School of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation
Laboratory of Chemoautotrophic Biosynthesis, Institute of Biophysics, SB, RAS, Federal Research Center, Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Kiselev, E. G.; Zhila, N. O.; Volova, T. G.

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10.

Properties of degradable polyhydroxyalkanoates with different monomer compositions / T. Volova, E. Kiselev, I. Nemtsev [et al.] // Int. J. Biol. Macromol. - 2021. - Vol. 182. - P98-114, DOI 10.1016/j.ijbiomac.2021.04.008. - Cited References:106. - The part of the reported study on polymer synthesis and examinationwas funded by RFBR and KKRF [Grant No. 19-43-240012 "Biological and physical principles of production of new generation biomaterials"]. The work on production and investigation of polymer films was carried out as part of the State Assignment of the Ministry of Education and Science of the Russian Federation [Grant No. FSRZ-2020-0006]. . - ISSN 0141-8130. - ISSN 1879-0003

РУБ Biochemistry & Molecular Biology + Chemistry, Applied + Polymer Science
Рубрики:
PALM KERNEL OIL
RALSTONIA-EUTROPHA
BIODEGRADABLE POLYMERS
Кл.слова (ненормированные):
Polyhydroxyalkanoates -- Chemical composition -- Physicochemical properties -- Films -- Microstructure -- Surface properties
Аннотация: Purpose: To synthesize and investigate polyhydroxyalkanoates (PHAs) with different monomer composition and percentages and polymer films prepared from them. Results: Various PHAs: homopolymer poly-3-hydroxybutyrate P(3HB) and 2-, 3-, and 4-component copolymers comprising various combinations of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), 4-hydroxybutyrate (4HB), and 3-hydroxyhexanoate (3HHx) monomers were synthesized under specialized conditions. Relationships were found between the monomer composition of PHAs and their molecular-weight and thermal properties and degree of crystallinity. All copolymers had decreased weight average molecular weights, Mw (to 390-600 kDa), and increased values of polydispersity (3.2-4.6) compared to the P(3HB). PHA copolymers showed different thermal behavior: an insignificant decrease in Tmelt and the presence of the second peak in the melting region and changes in parameters of crystallization and glass transition. At the same time, they retained thermostability, and the difference between Tmelt and Tdegr was at least 100-120 degrees C. Incorporation of 4HB, 3HV, and 3HHx monomer units into the 3-hydroxybutyrate chain caused changes in the amorphous to crystalline ratio and decreased the degree of crystallinity (Cx) to 20-40%. According to the degree to which the monomers reduced crystallinity, they were ranked as follows: 4HB - 3HHx - 3HV. A unique set of films was produced; their surface properties and physical/mechanical properties were studied as dependent on PHA composition; monomers other than 3hydroxybutyrate were found to enhance hydrophilicity, surface development, and elasticity of polymer films. Conclusion: An innovative set of PHA copolymers was synthesized and solution-cast films were prepared from them; the copolymers and films were investigated as dependent on polymer chemical composition. Results obtained in the present study contribute to the solution of a critical issue of producing degradable polymer materials. (C) 2021 Elsevier B.V. All rights reserved.

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Держатели документа:
Siberian Fed Univ, 79 Svohodnyi Av, Krasnoyarsk 660041, Russia.
SB RAS, Krasnoyarsk Sci Ctr, Fed Res Ctr, Inst Biophys, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
SB RAS, Krasnoyarsk Sci Ctr, Fed Res Ctr, 50 Akademgorodok, Krasnoyarsk 660036, Russia.
SB RAS, Krasnoyarsk Sci Ctr, Fed Res Ctr, LV Kirensky Inst Phys, 50-12 Akademgorodok, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Volova, T.; Kiselev, E.; Nemtsev, I.; Lukyanenko, A.; Sukovatyi, A.; Kuzmin, A.; Ryltseva, G.; Shishatskaya, E.; Petrovich, Kuzmin Andrey; RFBRRussian Foundation for Basic Research (RFBR) [19-43-240012]; Ministry of Education and Science of the Russian FederationMinistry of Education and Science, Russian Federation [FSRZ-2020-0006]

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11.

Laser Processing of Polymer Films Fabricated from PHAs Differing in Their Monomer Composition / T. G. Volova, A. I. Golubev, I. V. Nemtsev [et al.] // Polymers. - 2021. - Vol. 13, Is. 10. - Ст. 1553, DOI 10.3390/polym13101553. - Cited References:87. - This work was supported by the Russian Foundation for Basic Research/Regional State Autonomous Institution "Krasnoyarsk Regional Fund for the Support of Scientific and ScientificTechnical Activities" foundations under Grant number 19-43-240012 (laser treatment and films properties) and by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (polymer synthesis). . - ISSN 2073-4360

РУБ Polymer Science
Рубрики:
CHEMOMECHANICAL PROPERTIES
PHYSICOCHEMICAL PROPERTIES
SURFACE
Кл.слова (ненормированные):
P(3HB) -- copolymers -- films -- CO2 laser -- SEM -- AFM -- water contact angles -- MTT assay
Аннотация: The study reports results of using a CO2-laser in continuous wave (3 W; 2 m/s) and quasi-pulsed (13.5 W; 1 m/s) modes to treat films prepared by solvent casting technique from four types of polyhydroxyalkanoates (PHAs), namely poly-3-hydroxybutyrate and three copolymers of 3-hydroxybutyrate: with 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate (each second monomer constituting about 30 mol.%). The PHAs differed in their thermal and molecular weight properties and degree of crystallinity. Pristine films differed in porosity, hydrophilicity, and roughness parameters. The two modes of laser treatment altered these parameters and biocompatibility in diverse ways. Films of P(3HB) had water contact angle and surface energy of 92 degrees and 30.8 mN/m, respectively, and average roughness of 144 nm. The water contact angle of copolymer films decreased to 80-56 degrees and surface energy and roughness increased to 41-57 mN/m and 172-290 nm, respectively. Treatment in either mode resulted in different modifications of the films, depending on their composition and irradiation mode. Laser-treated P(3HB) films exhibited a decrease in water contact angle, which was more considerable after the treatment in the quasi-pulsed mode. Roughness parameters were changed by the treatment in both modes. Continuous wave line-by-line irradiation caused formation of sintered grooves on the film surface, which exhibited some change in water contact angle (76-80 degrees) and reduced roughness parameters (to 40-45 mN/m) for most films. Treatment in the quasi-pulsed raster mode resulted in the formation of pits with no pronounced sintered regions on the film surface, a more considerably decreased water contact angle (to 67-76 degrees), and increased roughness of most specimens. Colorimetric assay for assessing cell metabolic activity (MTT) in NIH 3T3 mouse fibroblast culture showed that the number of fibroblasts on the films treated in the continuous wave mode was somewhat lower; treatment in quasi-pulsed radiation mode caused an increase in the number of viable cells by a factor of 1.26 to 1.76, depending on PHA composition. This is an important result, offering an opportunity of targeted surface modification of PHA products aimed at preventing or facilitating cell attachment.

WOS
Держатели документа:
Siberian Fed Univ, Sch Fundamental Biol & Biotechnol, Basic Dept Biotechnol, 79 Svobodnyi Av, Krasnoyarsk 660041, Russia.
Krasnoyarsk Sci Ctr SB RAS, Inst Biophys SB RAS, Fed Res Ctr, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
Krasnoyarsk Sci Ctr SB RAS, LV Kirensky Inst Phys SB RAS, Fed Res Ctr, 50-38 Akademgorodok, Krasnoyarsk 660036, Russia.
Krasnoyarsk Sci Ctr SB RAS, Special Design & Technol BureauNaukaFed Res Ctr, 50-45 Akademgorodok, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Siberian Branch, Krasnoyarsk Sci Ctr, Fed Res Ctr, 50 Akademgorodok, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Volova, Tatiana G.; Golubev, Alexey, I; Nemtsev, Ivan, V; Lukyanenko, Anna, V; Dudaev, Alexey E.; Shishatskaya, Ekaterina, I; Russian Foundation for Basic Research/Regional State Autonomous Institution "Krasnoyarsk Regional Fund for the Support of Scientific and ScientificTechnical Activities" foundations [19-43-240012]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]

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12.

Laser processing of polymer films fabricated from phas differing in their monomer composition / T. G. Volova, A. I. Golubev, I. V. Nemtsev [et al.] // Polym. - 2021. - Vol. 13, Is. 10. - Ст. 1553, DOI 10.3390/polym13101553 . - ISSN 2073-4360
Кл.слова (ненормированные):
AFM -- CO2 laser -- Copolymers -- Films -- MTT assay -- NIH 3T3 fibroblasts -- P(3HB) -- SEM -- Water contact angles -- Biocompatibility -- Carbon dioxide lasers -- Contact angle -- Continuous cell culture -- Crystallinity -- Fibroblasts -- Interfacial energy -- Irradiation -- Monomers -- Organic polymers -- Pulsed lasers -- Sintering -- Surface roughness -- 3t3 mouse fibroblasts -- Continuous wave modes -- Degree of crystallinity -- Monomer compositions -- Poly-3-hydroxybutyrate -- Polyhydroxyalkanoates -- Roughness parameters -- Water contact angle -- Polymer films
Аннотация: The study reports results of using a CO2-laser in continuous wave (3 W; 2 m/s) and quasi-pulsed (13.5 W; 1 m/s) modes to treat films prepared by solvent casting technique from four types of polyhydroxyalkanoates (PHAs), namely poly-3-hydroxybutyrate and three copolymers of 3-hydroxybutyrate: with 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate (each second monomer constituting about 30 mol.%). The PHAs differed in their thermal and molecular weight properties and degree of crystallinity. Pristine films differed in porosity, hydrophilicity, and roughness parameters. The two modes of laser treatment altered these parameters and biocompatibility in diverse ways. Films of P(3HB) had water contact angle and surface energy of 92? and 30.8 mN/m, respectively, and average roughness of 144 nm. The water contact angle of copolymer films decreased to 80–56? and surface energy and roughness increased to 41–57 mN/m and 172–290 nm, respectively. Treatment in either mode resulted in different modifications of the films, depending on their composition and irradiation mode. Laser-treated P(3HB) films exhibited a decrease in water contact angle, which was more considerable after the treatment in the quasi-pulsed mode. Roughness parameters were changed by the treatment in both modes. Continuous wave line-by-line irradiation caused formation of sintered grooves on the film surface, which exhibited some change in water contact angle (76–80? ) and reduced roughness parameters (to 40–45 mN/m) for most films. Treatment in the quasi-pulsed raster mode resulted in the formation of pits with no pronounced sintered regions on the film surface, a more considerably decreased water contact angle (to 67–76? ), and increased roughness of most specimens. Colorimetric assay for assessing cell metabolic activity (MTT) in NIH 3T3 mouse fibroblast culture showed that the number of fibroblasts on the films treated in the continuous wave mode was somewhat lower; treatment in quasi-pulsed radiation mode caused an increase in the number of viable cells by a factor of 1.26 to 1.76, depending on PHA composition. This is an important result, offering an opportunity of targeted surface modification of PHA products aimed at preventing or facilitating cell attachment. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Scopus
Держатели документа:
Basic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
L.V. Kirensky Institute of Physics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/38 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Special Design and Technological Bureau ‘Nauka’ Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/45 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences”, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Volova, T. G.; Golubev, A. I.; Nemtsev, I. V.; Lukyanenko, A. V.; Dudaev, A. E.; Shishatskaya, E. I.

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13.

Cupriavidus necator B-10646 growth and polyhydroxyalkanoates production on different plant oils / T. Volova, K. Sapozhnikova, N. Zhila // Int. J. Biol. Macromol. - 2020. - Vol. 164. - P121-130, DOI 10.1016/j.ijbiomac.2020.07.095. - Cited References:52. - This studywas financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 074-02-2018-328) in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning" (polymer synthesis fromplant oils), and by the State assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (polymer properties). . - ISSN 0141-8130. - ISSN 1879-0003

РУБ Biochemistry & Molecular Biology + Chemistry, Applied + Polymer Science
Рубрики:
FATTY-ACID-COMPOSITION
PHA SYNTHASE GENE
PALM KERNEL OIL
Кл.слова (ненормированные):
Cupriavidus necator B-10646 -- Plant oils -- Polyhydroxyalkanoates -- Fatty -- acids -- Emulsifiers
Аннотация: The study addresses the growth of the wild-type strain Cupriavidus necator B-10646 and synthesis of polyhydroxyalkanoates by this strain on media containing plant oils with different compositions of fatty acids: palm, Siberian oilseed, and refined and unrefined sunflower seed oils. The study showed that the best carbon substrate was palm oil. Comparison of fatty acid compositions of the starting oils and unutilized residual substrates showed that C. necator B-10646 cells consumed the fatty acids from palm oil evenly while in experiments with other oils, they utilized polyenoic fatty acids first. Higher production parameters of the culture were obtained by preparation of emulsified oil medium using Tween 80 and sodium cocoyl glutamate as emulsifiers. All polyhydroxyalkanoate specimens were terpolymers that contained 3-hydroxybutyrate as the major component and minor amounts of 3-hydroxyvalerate (0.9-1.9 mol%) and 3-hydroxyhexanoate (0.5-1.1 mol%). Molecular weight of polyhydroxyalkanoate specimens depended on the type of plant oil and emulsifier. (C) 2020 Elsevier B.V. All rights reserved.

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Держатели документа:
Siberian Fed Univ, 79 Svobodny Pr, Krasnoyarsk 660041, Russia.
Krasnoyarsk Sci Ctr SB RAS, Inst Biophys SB RAS, Fed Res Ctr, 50-50 Akad Gorodok, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Volova, Tatiana; Sapozhnikova, Kristina; Zhila, Natalia; Project "Agropreparations of the new generation: a strategy of construction and realization" [074-02-2018-328]; Government of the Russian Federation; State assignment of the Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]

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14.

Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by cupriavidus necator B-10646 from mixtures of oleic acid and 3-hydroxyvalerate precursors / N. O. Zhila, G. S. Kalacheva, V. V. Fokht [и др.] // J. Sib. Fed. Univ. - Biol. - 2020. - Vol. 13, Is. 3. - С. 331-341, DOI 10.17516/1997-1389-0320 . - ISSN 1997-1389
Кл.слова (ненормированные):
3-hydroxyvalerate -- Cupriavidus necator -- Fatty acids -- Molecular weight -- oleic acid
Аннотация: Polyhydroxyalkanoates have attracted much attention as biodegradable alternative to petroleum-based synthetic plastics. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] copolymer is one of the best characterized PHA copolymers because of its high commercial potential. However, commercial use of PHAs has been limited by their high price. One approach to reducing the cost of PHA production is to use inexpensive carbon sources (fatty acids, plant oils, etc.). The aim of this work was to study synthesis of P(3HB-co-3HV) by the Cupriavidus necator B-10646 bacterium grown on oleic acid and different biochemical precursors of 3HV. Bacterial cells were grown for 72 h at 30°C and 200 rpm on an incubator shaker. Salts of propionic or valeric acids were used as precursors of 3HV. The content and the composition of the polymer were determined by gas chromatography of fatty acid methyl esters. Lipids and polymer were extracted from biomass using the method of Folch. The addition of potassium propionate and valerate did not inhibit bacterial growth and polymer synthesis, the cell concentration and polymer content reaching 9.3-9.5 g/L and 80-83%, respectively. The addition of potassium valerate or propionate led to the synthesis of (P(3HB-co-3HV)) copolymer containing 21.2 and 14.3 mol% of 3HV, respectively. The number average molecular weight (Mn) of the polymer synthesized by the bacterium on oleic acid alone was 220 kDa; the polydispersity of the polymer was 3.5. The polymer synthesized in the presence of potassium valerate and propionate was characterized by a lower Mn (156-178 kDa) and a higher polydispersity of the polymer (4.4-4.9). The main fatty acids (FA) of intracellular lipids were oleic (33.26% of the total FA) and palmitic acid (27.48% of the total FA). The addition of potassium propionate or valerate did not cause any significant changes in the composition of the FA of intracellular lipids of the strain studied. This study demonstrates the ability of C. necator B-10646 to synthesize P(3HB-co-3HV) from mixtures of oleic acid and 3HV precursors. The data obtained can be used to develop and implement an economically feasible process of the P(3HB-co-3HV) production. © Siberian Federal University. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, FRC Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Zhila, N. O.; Kalacheva, G. S.; Fokht, V. V.; Bubnova, S. S.; Volova, T. G.

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15.

Synthesis of Polyhydroxyalkanoates by Hydrogen-Oxidizing Bacteria in a Pilot Production Process / T. Volova [et al.] // Biomacromolecules. - 2019, DOI 10.1021/acs.biomac.9b00295 . - Article in press. - ISSN 1525-7797
Кл.слова (ненормированные):
Crystallinity -- Fructose -- Glucose -- Pilot plants -- Purification -- Substrates -- Terpolymers -- Carbon substrates -- Cell concentrations -- Degree of crystallinity -- Hydrogen-oxidizing bacteria -- Polies (3 hydroxybutyrate co 3 hydroxyvalerate co 4 hydroxybutyrate) -- Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) -- Polyhydroxyalkanoates -- Yield coefficient -- Glycerol
Аннотация: The synthesis of polyhydroxyalkanoates (PHAs) was scaled up to pilot production in a 150-L fermenter on sugars (fructose and glucose) and purified and crude glycerol in a culture of the wild-type strain Cupriavidus necator B-10646. Over 60 h of cultivation, a cell concentration of 150-160 g/L was obtained on purified glycerol and glucose; cultivation on fructose and crude glycerol resulted in a cell concentration of 130 ± 10 g/L. Polymer content and yield coefficients for the biomass were similar on all substrates (80-85 wt % and 0.29-0.33 kg biomass/kg carbon substrate, respectively). Copolymers poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and poly(3-hydroxybutyrate-co-4-hydroxybutyrate) and terpolymers poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxybutyrate) having a decreased degree of crystallinity (36-46%) were first synthesized in the scaled-up process using C. necator B-10646 cultivated on glycerol. These results will provide the basis for scaling-up PHA synthesis in an organotrophic C. necator B-10646 culture. © 2019 American Chemical Society.

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Держатели документа:
Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center Krasnoyarsk Science Center SB RAS, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Volova, T.; Kiselev, E.; Zhila, N.; Shishatskaya, E.

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16.

The effect of the chemical composition and structure of polymer films made from resorbable polyhydroxyalkanoates on blood cell response / E. I. Shishatskaya, N. G. Menzyanova, A. A. Shumilova // Int. J. Biol. Macromol. - 2019. - Vol. 141. - P765-774, DOI 10.1016/j.ijbiomac.2019.09.015 . - ISSN 0141-8130
Кл.слова (ненормированные):
Blood cell response -- Degradable polyhydroxyalkanoates (PHAs) -- Surface structure and properties
Аннотация: Four PHA types were synthesized in the culture of Cupriavidus eutrophus B-10646 under special conditions, poly(3?hydroxybutyrate) [P(3HB)] and of copolymers, which contained 3HB monomers and 4?hydroxybutyrate (4HB), 3?hydroxyvalerate (3HV), or 3?hydroxyhexanoate (3HHx). All copolymers had the Mw of about 550–670 kDa, and the homopolymer P(3HB) had a significantly higher Mw – 920 kDa. P(3HB?co?4HB) and P(3HB?co?3HHx) had the lowest Cx (42 and 49%) while P(3HB?co?3HV) and P(3HB) exhibited higher Cx values (76%). Polymer films were prepared from different PHAs. Electron microscopy showed differences in the surface microstructure of the films. Films prepared from the P(3HB) were more hydrophobic and the arithmetic mean surface roughness of 71–75 nm, than the copolymer films, which were hydrophilic (57–60°) and had considerably higher roughness (158–177 nm). Blood parameters (hemoglobin and hemolysis) and response of the cells (erythrocytes, platelets, and monocytes) were studied in experiments with blood directly contacting the surface of the films of PHAs with different compositions. Cultivation of blood cells on polymer films did not cause any adverse effects on adhesion and morphology of all cell types. Results of studying blood cell response suggested that the films made from low-crystallinity copolymers containing 4?hydroxybutyrate and 3?hydroxyhexanoate were the best for contact with blood. © 2019 Elsevier B.V.

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Держатели документа:
Siberian Federal University, 79 Svobodnyi Av, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Shishatskaya, E. I.; Menzyanova, N. G.; Shumilova, A. A.

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17.

РУБ Biochemistry & Molecular Biology + Chemistry, Applied + Polymer Science
Рубрики:
3-HYDROXYBUTYRATE
   BIOCOMPATIBILITY
   BIOSYNTHESIS
   ADHESION
   STENTS
Кл.слова (ненормированные):
Degradable polyhydroxyalkanoates (PHAs) -- Surface structure and -- properties -- Blood cell response
Аннотация: Four PHA types were synthesized in the culture of Cupriavidus eutrophus B-10646 under special conditions, poly(3 hydroxybutyrate) [P(3HB)] and of copolymers, which contained 3HB monomers and 4 hydroxybutyrate (4HB), 3 hydroxyvalerate (3HV), or 3 hydroxyhexanoate (3HHx). All copolymers had the M-w of about 550-670 kDa, and the homopolymer P(3HB) had a significantly higher M-w - 920 kDa. P(3HB co 4HB) and P(3HB co 3HHx) had the lowest C-x (42 and 49%) while P(3HB co 3HV) and P(3HB) exhibited higher C-x values (76%). Polymer films were prepared from different PHAs. Electron microscopy showed differences in the surface microstructure of the films. Films prepared from the P(3HB) were more hydrophobic and the arithmetic mean surface roughness of 71-75 nm, than the copolymer films, which were hydrophilic (57-60 degrees) and had considerably higher roughness (158-177 nm). Blood parameters (hemoglobin and hemolysis) and response of the cells (erythrocytes, platelets, and monocytes) were studied in experiments with blood directly contacting the surface of the films of PHAs with different compositions. Cultivation of blood cells on polymer films did not cause any adverse effects on adhesion and morphology of all cell types. Results of studying blood cell response suggested that the films made from low-crystallinity copolymers containing 4 hydroxybutyrate and 3 hydroxyhexanoate were the best for contact with blood. (C) 2019 Elsevier B.V. All rights reserved.

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Держатели документа:
Siberian Fed Univ, 79 Svobodnyi Ave, Krasnoyarsk 660041, Russia.
RAS, SB, Krasnoyarsk Sci Ctr, Inst Biophys,Fed Res Ctr, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Shishatskaya, Ekaterina I.; Menzyanova, Natalia G.; Shumilova, Anna A.; Russian Science FoundationRussian Science Foundation (RSF) [17-15-01352]

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18.

Polyhydroxyalkanoates (PHA) for therapeutic applications / J. Y. Zhang [et al.] // Mater. Sci. Eng. C-Mater. Biol. Appl. - 2018. - Vol. 86. - P144-150, DOI 10.1016/j.msec.2017.12.035. - Cited References:105. - This research was financially supported by a grant from Ministry of Sciences and Technology (Grant No. 2016YFB0302504); grants from National Natural Science Foundation of China (Grant No. 31430003, 31600072 and 81503079); a grant from Natural Science Foundation of Jiangxi Province (Grant No. 20161BAB215204); and a grant from Russian Science Foundation (Grant No. 17-15-01352). Tsinghua President Fund also supported this project (Grant No. 2015THZ10). . - ISSN 0928-4931. - ISSN 1873-0191

РУБ Materials Science, Biomaterials
Рубрики:
TISSUE ENGINEERING APPLICATIONS
3-HYDROXYBUTYRATE METHYL-ESTER
Кл.слова (ненормированные):
PHA -- Therapeutics -- Tissue engineering -- Implants -- Drug delivery -- Biomedicine
Аннотация: As intracellular carbon and energy storage materials, polyhydroxyalkanoates (PHA) are a diverse biopolyesters synthesized by many bacteria. PHA have been produced in large quantity for various application research including medical implants for approximately 30 years. Many studies demonstrated that PHA are promising implant materials due to their diverse and ascendant mechanical, biodegradable and tissue compatible properties. Importantly, common PHA biodegradation products including oligomers and monomers are also not toxic to the cells and tissues. Pharmaceutical applications of some PHA degradation products also have been reported. So far, no study has been reported to have any carcinogenesis result induced by any PHA or their biodegradation products. All results suggest that PHA could be developed into various bio-implant products.

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Держатели документа:
Nanchang Univ, Inst Life Sci, Lab Fear & Anxiety Disorders, Nanchang 330031, Jiangxi, Peoples R China.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
Tsinghua Univ, Sch Life Sci, MOE Key Lab Bioinformat, Beijing 100084, Peoples R China.
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia.
Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05509900 Sao Paulo, Brazil.

Доп.точки доступа:
Zhang, Junyu; Shishatskaya, Ekaterina I.; Volova, Tatiana G.; da Silva, Luiziana Ferreira; Chen, Guo-Qiang; Ministry of Sciences and Technology [2016YFB0302504]; National Natural Science Foundation of China [31430003, 31600072, 81503079]; Natural Science Foundation of Jiangxi Province [20161BAB215204]; Russian Science Foundation [17-15-01352]; Tsinghua President Fund [2015THZ10]

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19.

Properties of PHA bi-, ter-, and quarter-polymers containing 4-hydroxybutyrate monomer units / N. Zhila, E. Shishatskaya // Int. J. Biol. Macromol. - 2018. - Vol. 111. - P1019-1026, DOI 10.1016/j.ijbiomac.2018.01.130. - Cited References:39. - The research was supported by the Russian Science Foundation (grant No. 17-15-01352). . - ISSN 0141-8130. - ISSN 1879-0003

РУБ Biochemistry & Molecular Biology + Chemistry, Applied + Polymer Science
Рубрики:
ALCALIGENES-FAECALIS T1
COMAMONAS-ACIDOVORANS
ENZYMATIC DEGRADATION
Кл.слова (ненормированные):
Polyhydroxyalkanoates -- 4-Hydroxybutyrate monomer units -- Physicochemical -- and mechanical properties
Аннотация: The present study investigates physicochemical, mechanical, and biological properties of polyhydroxyalkanoate (PHA) copolymers containing 4-hydroxybutyrate (4HB) synthesized in Cupriavidus eutrophus B10646 culture. In poly(3-hydroxybutyrate/4-hydroxybutyrate) [P(3HB/4HB)]bipolymers, 4HB varied between 10.4 and 75.0 mol%; in poly(3-hydroxybutyrate/3-hydroxyvalerate/4-hydroxybutyrate) terpolymers, 4HB constituted 28.7-55.6 mol%; and in poly(3-hydroxybutyrate/3-hydroxyvalerate/4-hydroxybutyrate/3-hydroxyhexanoate) quaterpolymers, 4HB varied between 9.3 and 13.3 mol%. The degree of crystallinity of P(3HB/4HB) copolymers decreased consistently with an increase in 4HB content, reaching 38%. The incorporation of 3-hydroxyvalerate and 3-hydroxyhexanoate into copolymers enhanced that effect. The effect of 4HB monomer units on temperature properties of copolymers was exhibited as lowering of the melting temperature and crystallization temperature, which improved the processing-related properties of the copolymers. All copolymers containing 4HB showed enhanced elongation at break compared to poly(3-hydroxybutyrate). Polymer films prepared from PHA5 with different chemical composition had similar microstructure and porosity and had no toxic effect on mouse fibroblast NIH 3 T3 cells, proving their high biocompatibility. (C) 2018 Elsevier B.V. All rights reserved.

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Держатели документа:
Siberian Fed Univ, 79 Svobodnyi Ave, Krasnoyarsk 660041, Russia.
RAS, Krasnoyarsk Sci Ctr, Fed Res Ctr, Inst Biophys,SB, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Zhila, Natalia; Shishatskaya, Ekaterina; Russian Science Foundation [17-15-01352]

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20.

Novel spray-dried PHA microparticles for antitumor drug release / A. Shershneva [et al.] // Dry. Technol. - 2018. - Vol. 36, Is. 11. - P1387-1398, DOI 10.1080/07373937.2017.1407940. - Cited References:67. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences: [Project no. AAAA-A17- 117013050028-8]. . - ISSN 0737-3937. - ISSN 1532-2300

РУБ Engineering, Chemical + Engineering, Mechanical
Рубрики:
DRYING METHOD
   IN-VITRO
   BIODEGRADABLE NANOPARTICLES
   ORAL
Кл.слова (ненормированные):
5-Fluorouracil -- efficiency -- microparticles -- paclitaxel -- polyhydroxyalkanoates -- spray-drying
Аннотация: The production of poly-3-hydroxybutyrate (P3HB) and poly-3-hydroxybutyrate/polyethylene glycol (PEG)-based microparticles, loaded with antitumor drugs paclitaxel (PTX) and 5-Fluorouracil (5-FU) by spray-drying technique, was investigated. The average diameter of microparticles was found to be 3.4 +/- 0.5 pm and zeta potential was about -44 mV. The addition of surfactant PEG did not show any effect on the morphological characteristics of the particles. But the chemical structure of drug influenced on the properties. Microparticles had heterogeneous pores on the surface when the hydrophobic PTX was encapsulated. It was established that the addition of surfactant positively influenced on the properties of particles and led to the loading of 5-FU directly into the matrix. This is confirmed by the results of electron microscopy and dynamics of drug release in vitro. As a whole, the release profiles of PTX and 5-FU from composite P3HB/PEG microparticles were less than from P3HB microparticles. The results of the morphological evaluation of Hela cells demonstrated that the use of cytostatic drugs loaded in P3HB microparticles induces morphological changes associated with apoptosis (chromatin condensation, core fragmentation, margination of nucleus). Thus, the obtained results can serve as the basis for the development of new antitumor drugs of prolonged action, intended for various modes of administration.

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Держатели документа:
Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, 79 Svobodny, Krasnoyarsk 660041, Russia.
SB RAS, Inst Biophys, Fed Res Ctr, Krasnoyarsk Sci Ctr, Tomsk, Russia.

Доп.точки доступа:
Shershneva, Anna; Murueva, Anastasiya; Nikolaeva, Elena; Shishatskaya, Ekaterina; Volova, Tatiana; Russian Academy of Sciences [AAAA-A17- 117013050028-8]

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