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Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Волова Т.Г.
Заглавие : Биотехнология водородокисляющих микроорганизмов : научное издание
Место публикации : Материалы международной научной конференции "Автотрофные микроорганизмы", посвященной 75-летию со дня рождения академика Е.Н. Кондратьевой, Москва, 13-15 декабря, 2000. - М., 2000. - С. 39-40. - ISBN 5-317-00100-5
ГРНТИ : 34.27.39
Предметные рубрики: ВОДОРОДНЫЕ БАКТЕРИИ
ALCALIGENES EUTROPHUS (BACT.)
БЕЛОК ОДНОКЛЕТОЧНЫХ
ГИДРОГЕНАЗЫ
CO-ОКСИДАЗЫ
ЦИТОХРОМЫ
ПОЛИОКСИАЛКАНОАТЫ
ПОЛУЧЕНИЕ
Аннотация: Практический интерес к водородокисляющим бактериям возник в шестидесятые годы как потенциальному регенеративному звену в системах жизнеобеспечения человека (СЖО), а также продуценту белка одноклеточных. Перспективной в этом отношении является всесторонне исследованная культура Alcaligenes eutrophus. Разработана технология и создано опытное производство белка одноклеточных на водороде. В результате комплексных испытаний подтверждена высокая биологическая ценность полученного нового белкового препарата и эффективность применения биомассы водородных бактерий в качестве кормового белка. Отобраны и исследованы штаммы, резистентные к окиси углерода, выявлена специфика бактериальной устойчивости к CO. Обоснована применимость для выращивания бактерий техногенных источников водорода (конвертированного газа и водородсодержащих продуктов переработки низкосортных бурых углей и гидролизного лигнина). Отработаны технологические режимы для продукции гидрогеназ, CO-оксидазы, цитохромов. Показана возможность использования иммобилизированных клеток водородокисляющих микроорганизмов и ферментов для регенерации НАД, индикации H[2], связывания CO из воздуха. Исследованы закономерности синтеза водородными бактериями и карбоксидобактериями термопластичных биоразрушаемых полимеров оксипроизводных жирных к-т (полиоксиалканоатов), разработана и реализована лабораторная технология получения полиоксиалканоатов на основе водородного биосинтеза. Проводимые сегодня исследования ориентированы на оптимизацию технологии синтеза полиоксиалканоатов, включая клонирование phb- и pha-генов из Alcaligenes eutrophus и получение трансгенных суперпродуцентов разнообразных полимеров. Россия, Ин-т биофизики СО РАН, Красноярск
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Puzyr A.P., Mogilnaya O.A., Efremov S.N.
Заглавие : Tissue response to the implantation of biodegradable polyhydroxyalkanoate sutures
Место публикации : Journal of Materials Science: Materials in Medicine. - 2004. - Vol. 15, Is. 6. - С. 719-728. - ISSN 09574530 (ISSN) , DOI 10.1023/B:JMSM.0000030215.49991.0d
Ключевые слова (''Своб.индексиров.''): necrosis--polymeric implants--suppurative inflammation--tissue reaction--biodegradation--calcification (biochemistry)--cells--copolymers--implants (surgical)--silk--tissue--tumors--materials science--acid phosphatase--copolymer--poly(3 hydroxybutyric acid)--polyhydroxyalkanoic acid--polyhydroxyvaleric acid--unclassified drug--animal experiment--animal model--article--biodegradable implant--blood vessel reactivity--catgut--controlled study--enzyme activity--female--giant cell--histochemistry--inflammation--macrophage--nonhuman--priority journal--rat--silk--suture--tensile strength--tissue reaction--tissue structure--wound healing--young modulus--absorbable implants--animals--female--fibrosis--foreign-body reaction--hydroxybutyrates--muscle, skeletal--polyesters--polymers--rats--rats, wistar--sutures--treatment outcome--wounds, penetrating--animalia
Аннотация: Polyhydroxyalkanoate (PHA) sutures were implanted to test animals intramuscularly, and tissue reaction was investigated and compared with the reaction to silk and catgut. Tested monofilament sutures made of PHAs of two types polyhydroxybutyrate (PHB) and a copolymer of hydroxybutyrate and hydroxyvalerate (PHV) featured the strength necessary for the healing of muscle-fascial wounds. The reaction of tissues to polymeric implants was similar to their reaction to silk and was less pronounced than the reaction to catgut; it was expressed in a transient post-traumatic inflammation (up to four weeks) and the formation of a fibrous capsule less than 200 ?m thick, which became as thin as 4060 ?m after 16 weeks, in the course of reverse development. Macrophages and foreign-body giant cells with a high activity of acid phosphatase were actively involved in this process. PHB and PHB/PHV sutures implanted intramuscularly for an extended period (up to one year) did not cause any acute vascular reaction at the site of implantation or any adverse events, such as suppurative inflammation, necrosis, calcification of the fibrous capsule or malignant tumor formation. No statistically significant differences were revealed in the tissue response to polymer sutures of the two types. Capsules around silk and catgut sutures did not become significantly thinner.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Puzyr A.P., Mogilnaya O.A., Efremov S.N.
Заглавие : Tissue response to the implantation of biodegradable polyhydroxyalkanoate sutures
Колич.характеристики :10 с
Место публикации : J. Mater. Sci.-Mater. Med.: KLUWER ACADEMIC PUBL, 2004. - Vol. 15, Is. 6. - P719-728. - ISSN 0957-4530, DOI 10.1023/B:JMSM.0000030215.49991.0d
Примечания : Cited References: 34
Предметные рубрики: DEGRADATION
POLYESTERS
POLYMERS
FIBERS
PHB
Аннотация: Polyhydroxyalkanoate (PHA) sutures were implanted to test animals intramuscularly, and tissue reaction was investigated and compared with the reaction to silk and catgut. Tested monofilament sutures made of PHAs of two types-polyhydroxybutyrate (PHB) and a copolymer of hydroxybutyrate and hydroxyvalerate (PHV)-featured the strength necessary for the healing of muscle-fascial wounds. The reaction of tissues to polymeric implants was similar to their reaction to silk and was less pronounced than the reaction to catgut; it was expressed in a transient post-traumatic inflammation (up to four weeks) and the formation of a fibrous capsule less than 200 mum thick, which became as thin as 40-60 mum after 16 weeks, in the course of reverse development. Macrophages and foreign-body giant cells with a high activity of acid phosphatase were actively involved in this process. PHB and PHB/PHV sutures implanted intramuscularly for an extended period (up to one year) did not cause any acute vascular reaction at the site of implantation or any adverse events, such as suppurative inflammation, necrosis, calcification of the fibrous capsule or malignant tumor formation. No statistically significant differences were revealed in the tissue response to polymer sutures of the two types. Capsules around silk and catgut sutures did not become significantly thinner. (C) 2004 Kluwer Academic Publishers.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S.
Заглавие : The synthesis of hydroxybutyrate and hydroxyvalerate copolymers by the bacterium Ralstonia eutropha
Место публикации : Mikrobiologiya. - 2005. - Vol. 74, Is. 1. - С. 63-69. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): ?-ketothiolase--controlled synthesis--poly(hydroxybutyrate-co-hydroxyvalerate)--ralstonia eutropha--bacteria (microorganisms)--cupriavidus necator--acetoacetyl coenzyme a--acetyl coenzyme a acyltransferase--acyl coenzyme a--acyltransferase--butyric acid derivative--carbon dioxide--fructose--hydrogen--poly(3 hydroxybutyrate) co (3 hydroxyvalerate)--poly(3-hydroxyalkanoic acid) synthase--poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate)--polyester--polyhydroxyalkanoate synthase--valeric acid derivative--article--chemistry--crystallization--culture medium--metabolism--wautersia eutropha--acetyl-coa c-acyltransferase--acyl coenzyme a--acyltransferases--butyrates--carbon dioxide--crystallization--culture media--cupriavidus necator--fructose--hydrogen--polyesters--valerates
Аннотация: The paper deals with the study of the synthesis of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) copolymers by the bacterium Ralstonia eutropha B-5786 grown under different carbon nutrition conditions (growth on carbon dioxide, fructose, and CO2-valerate and fructose-valerate mixtures). The parameters to be analyzed included the yield of biomass, the yield, synthesis rate, and composition of copolymers, the activity of the key enzymes of polyhydroxyalkanoate (PHA) synthesis (?-ketothiolase, acetoacetyl-CoA reductase, and PHA synthase), the maximum tolerable concentration of valerate to the bacterium, and the conditions that govern the incorporation of hydroxyvalerate to copolymers. This allowed the relationship between cultivation conditions and the proportion of monomers in the copolymers to be deduced. We were able to synthesize a range of 3HB/3HV copolymers and found that the thermal characteristics and the degree of crystallinity of these copolymers depend on the molar fraction of 3HV.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S.
Заглавие : The synthesis of hydroxybutyrate and hydroxyvalerate copolymers by the bacterium Ralstonia eutropha
Место публикации : Microbiology. - 2005. - Vol. 74, Is. 1. - С. 54-59. - ISSN 00262617 (ISSN) , DOI 10.1007/s11021-005-0028-5
Ключевые слова (''Своб.индексиров.''): ?-ketothiolase--controlled synthesis--poly(hydroxybutyrate-co-hydroxyvalerate)--ralstonia eutropha--bacteria (microorganisms)--ralstonia--wautersia eutropha
Аннотация: The paper deals with the study of the synthesis of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) copolymers by the bacterium Ralstonia eutropha B-5786 grown under different carbon nutrition conditions (growth on carbon dioxide, fructose, and CO2-valerate and fructose-valerate mixtures). The parameters to be analyzed included the yield of biomass; the yield, synthesis rate, and composition of copolymers; the activity of the key enzymes of polyhydroxyalkanoate (PHA) synthesis ?-ketothiolase, acetoacetyl-CoA reductase, and PHA synthase); the maximum tolerable concentration of valerate to the bacterium; and the conditions that govern the incorporation of hydroxyvalerate into copolymers. This allowed the relationship between cultivation conditions and the proportion of monomers in the copolymers to be deduced. We were able to synthesize a range of 3HB/3HV copolymers and found that the thermal characteristics and the degree of crystallinity of such copolymers depend on the molar fraction of 3HV. В© 2005 Pleiades Publishing, Inc.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E. I., Menzyanova N. G., Shumilova A. A.
Заглавие : The effect of the chemical composition and structure of polymer films made from resorbable polyhydroxyalkanoates on blood cell response
Место публикации : Int. J. Biol. Macromol.: Elsevier B.V., 2019. - Vol. 141. - С. 765-774. - ISSN 01418130 (ISSN) , DOI 10.1016/j.ijbiomac.2019.09.015
Аннотация: Four PHA types were synthesized in the culture of Cupriavidus eutrophus B-10646 under special conditions, poly(3?hydroxybutyrate) [P(3HB)] and of copolymers, which contained 3HB monomers and 4?hydroxybutyrate (4HB), 3?hydroxyvalerate (3HV), or 3?hydroxyhexanoate (3HHx). All copolymers had the Mw of about 550–670 kDa, and the homopolymer P(3HB) had a significantly higher Mw – 920 kDa. P(3HB?co?4HB) and P(3HB?co?3HHx) had the lowest Cx (42 and 49%) while P(3HB?co?3HV) and P(3HB) exhibited higher Cx values (76%). Polymer films were prepared from different PHAs. Electron microscopy showed differences in the surface microstructure of the films. Films prepared from the P(3HB) were more hydrophobic and the arithmetic mean surface roughness of 71–75 nm, than the copolymer films, which were hydrophilic (57–60°) and had considerably higher roughness (158–177 nm). Blood parameters (hemoglobin and hemolysis) and response of the cells (erythrocytes, platelets, and monocytes) were studied in experiments with blood directly contacting the surface of the films of PHAs with different compositions. Cultivation of blood cells on polymer films did not cause any adverse effects on adhesion and morphology of all cell types. Results of studying blood cell response suggested that the films made from low-crystallinity copolymers containing 4?hydroxybutyrate and 3?hydroxyhexanoate were the best for contact with blood. © 2019 Elsevier B.V.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya, Ekaterina I., Menzyanova, Natalia G., Shumilova, Anna A.
Заглавие : The effect of the chemical composition and structure of polymer films made from resorbable polyhydroxyalkanoates on blood cell response
Колич.характеристики :11 с
Коллективы : Russian Science FoundationRussian Science Foundation (RSF) [17-15-01352]
Место публикации : Int. J. Biol. Macromol.: ELSEVIER, 2019. - Vol. 141. - С. 765-+. - ISSN 0141-8130, DOI 10.1016/j.ijbiomac.2019.09.015. - ISSN 1879-0003(eISSN)
Примечания : Cited References:57. - The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: The study is supporting by the Russian Science Foundation, Project No 17-15-01352.
Предметные рубрики: 3-HYDROXYBUTYRATE
BIOCOMPATIBILITY
BIOSYNTHESIS
ADHESION
STENTS
Аннотация: Four PHA types were synthesized in the culture of Cupriavidus eutrophus B-10646 under special conditions, poly(3 hydroxybutyrate) [P(3HB)] and of copolymers, which contained 3HB monomers and 4 hydroxybutyrate (4HB), 3 hydroxyvalerate (3HV), or 3 hydroxyhexanoate (3HHx). All copolymers had the M-w of about 550-670 kDa, and the homopolymer P(3HB) had a significantly higher M-w - 920 kDa. P(3HB co 4HB) and P(3HB co 3HHx) had the lowest C-x (42 and 49%) while P(3HB co 3HV) and P(3HB) exhibited higher C-x values (76%). Polymer films were prepared from different PHAs. Electron microscopy showed differences in the surface microstructure of the films. Films prepared from the P(3HB) were more hydrophobic and the arithmetic mean surface roughness of 71-75 nm, than the copolymer films, which were hydrophilic (57-60 degrees) and had considerably higher roughness (158-177 nm). Blood parameters (hemoglobin and hemolysis) and response of the cells (erythrocytes, platelets, and monocytes) were studied in experiments with blood directly contacting the surface of the films of PHAs with different compositions. Cultivation of blood cells on polymer films did not cause any adverse effects on adhesion and morphology of all cell types. Results of studying blood cell response suggested that the films made from low-crystallinity copolymers containing 4 hydroxybutyrate and 3 hydroxyhexanoate were the best for contact with blood. (C) 2019 Elsevier B.V. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Altukhova O.V.
Заглавие : The autotrophic synthesis of polyhydroxyalkanoate by alcaligenes eutrophus in the presence of carbon monoxide
Место публикации : Mikrobiologiya. - 2001. - Vol. 70, Is. 6. - С. 745-752. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): co inhibition--hydrogen-oxidizing bacteria--polyhydroxyalkanoates (phas)--synthesis--alkane--carbon monoxide--fatty acid--hydroxyacid--lipid--nitrogen--polyester--article--chemistry--culture medium--drug antagonism--growth, development and aging--mass spectrometry--metabolism--wautersia eutropha--alkanes--carbon monoxide--culture media--cupriavidus necator--fatty acids--hydroxy acids--lipids--mass spectrometry--nitrogen--polyesters
Аннотация: The CO-resistant strain B5786 of the hydrogen-oxidizing bacterium Alcaligenes eutrophus was found to be able to synthesize polyhydroxyalkanoates (PHAs) under the conditions of growth limitation by nitrogen deficiency (the factor that promotes PHA synthesis) and growth inhibition by carbon monoxide. The gas mixtures that contained from 5 to 20 vol % CO did not inhibit the key enzymes of PHA synthesis-?-ketothiolase, acetoacetyl-CoA reductase, hydroxybutyrate dehydrogenase, and PHA synthase. In the presence of CO, cells accumulated up to 70-75 wt % PHA (with respect to the dry biomass) without any noticeable increase in the consumption of the gas substrate. Chromatographic-mass spectrometric analysis showed that the PHA synthesized by A. eutrophus is a copolymer containing more than 99 mol % ?-hydroxybutyrate and trace amounts of ?-hydroxyvalerate. The PHA synthesized under the conditions described did not differ from that synthesized by A. eutrophus cells from electrolytic hydrogen.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Altukhova O.V.
Заглавие : The autotrophic synthesis of polyhydroxyalkanoate by Alcaligenes eutrophus in the presence of carbon monoxide
Место публикации : Microbiology. - 2001. - Vol. 70, Is. 6. - С. 640-646. - ISSN 00262617 (ISSN)
Ключевые слова (''Своб.индексиров.''): co inhibition--hydrogen-oxidizing bacteria--polyhydroxyalkanoates (phas)--synthesis
Аннотация: The CO-resistant strain B5786 of the hydrogen-oxidizing bacterium Alcaligenes eutrophus was found to be able to synthesize polyhydroxyalkanoates (PHAs) under the conditions of growth limitation by nitrogen deficiency (the factor that promotes PHA synthesis) and growth inhibition by carbon monoxide. The gas mixtures that contained from 5 to 20 vol % CO did not inhibit the key enzymes of PHA synthesis-?-ketothiolase, acetoacetyl-CoA reductase, hydroxybutyrate dehydrogenase, and PHA synthase. In the presence of CO, cells accumulated up to 70-75 wt % PHA (with respect to the dry biomass) without any noticeable increase in the consumption of the gas substrate. Chromatographic-mass spectrometric analysis showed that the PHA synthesized by A. eutrophus is a copolymer containing more than 99 mol % ?-hydroxybutyrate and trace amounts of ?-hydroxyvalerate. The PHA synthesized under the conditions described did not differ from that synthesized by A. eutrophus cells from electrolytic hydrogen. В© 2001 MAIK "Nauka/Interperiodica".
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Altukhova O.V.
Заглавие : The autotrophic synthesis of polyhydroxyalkanoate by Alcaligenes eutrophus in the presence of carbon monoxide
Колич.характеристики :7 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 2001. - Vol. 70, Is. 6. - P640-646. - ISSN 0026-2617, DOI 10.1023/A:1013175413013
Примечания : Cited References: 19
Ключевые слова (''Своб.индексиров.''): hydrogen-oxidizing bacteria--polyhydroxyalkanoates (phas)--synthesis--co inhibition
Аннотация: The CO-resistant strain B5786 of the hydrogen-oxidizing bacterium Alcaligenes eutrophus was found to be able to synthesize polyhydroxyalkanoates (PHAs) under the conditions of growth limitation by nitrogen deficiency (the factor that promotes PHA synthesis) and growth inhibition by carbon monoxide. The gas mixtures that contained from 5 to 20 vol % CO did not inhibit the key enzymes of PHA synthesis-beta-ketothiolase. acetoacetyl-CoA reductase, hydroxybutyrate dehydrogenase, and PHA synthase. In the presence of CO, cells accumulated up to 70-75 wt % PHA (with respect to the dry biomass) without any noticeable increase in the consumption of the gas substrate. Chromatographic-mass spectrometric analysis showed that the PHA synthesized by A. eutrophus is a copolymer containing more than 99 mol % beta-hydroxybutyrate and trace amounts of beta-hydroxyvalerate. The PHA synthesized under the conditions described did not differ from that synthesized by A. eutrophus cells from electrolytic hydrogen.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Kalacheva G.S., Rodicheva E.K., Volova T.G.
Заглавие : Synthesis of reserve polyhydroxyalkanoates by luminescent bacteria
Место публикации : Microbiology. - 2008. - Vol. 77, Is. 3. - С. 318-323. - ISSN 00262617 (ISSN) , DOI 10.1134/S0026261708030119
Ключевые слова (''Своб.индексиров.''): biosynthesis--chemical structure--luminescent bacteria--polyhydroxyalkanoates (pha)--bacteria (microorganisms)--photobacterium leiognathi--vibrio harveyi
Аннотация: The ability of marine luminescent bacteria to synthesize polyesters of hydroxycarboxylic acids (polyhydroxyalkanoates, PHA) as reserve macromolecules was studied. Twenty strains from the collection of the luminescent bacteria CCIBSO (WDCM839) of the Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, assigned to different taxa (Photobacterium leiognathi, Ph. phosphoreum, Vibrio harveyi, and V. fischeri) were analyzed. The most productive strains were identified, and the conditions ensuring high polymer yields in batch culture (40-70% of the cell dry mass weight) were determined. The capacity for synthesizing two-and three-component polymers containing hydroxybutyric acid as the main monomer and hydroxyvaleric and hydroxyhexanoic acids was revealed in Ph. leiognathi and V. harveyi strains. The results allow luminescent microorganisms to be regarded as new producers of multicomponent polyhydroxyalkanoates. В© 2008 MAIK Nauka.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T., Kiselev E., Zhila N., Shishatskaya E.
Заглавие : Synthesis of Polyhydroxyalkanoates by Hydrogen-Oxidizing Bacteria in a Pilot Production Process
Место публикации : Biomacromolecules: American Chemical Society, 2019. - Article in press. - ISSN 15257797 (ISSN) , DOI 10.1021/acs.biomac.9b00295
Аннотация: The synthesis of polyhydroxyalkanoates (PHAs) was scaled up to pilot production in a 150-L fermenter on sugars (fructose and glucose) and purified and crude glycerol in a culture of the wild-type strain Cupriavidus necator B-10646. Over 60 h of cultivation, a cell concentration of 150-160 g/L was obtained on purified glycerol and glucose; cultivation on fructose and crude glycerol resulted in a cell concentration of 130 ± 10 g/L. Polymer content and yield coefficients for the biomass were similar on all substrates (80-85 wt % and 0.29-0.33 kg biomass/kg carbon substrate, respectively). Copolymers poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and poly(3-hydroxybutyrate-co-4-hydroxybutyrate) and terpolymers poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxybutyrate) having a decreased degree of crystallinity (36-46%) were first synthesized in the scaled-up process using C. necator B-10646 cultivated on glycerol. These results will provide the basis for scaling-up PHA synthesis in an organotrophic C. necator B-10646 culture. © 2019 American Chemical Society.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Zhila N.O., Kalacheva G.S., Sokolenko V.A., Sinski E.J.
Заглавие : Synthesis of 3-hydroxybutyrate-CO-4-hydroxybutyrate copolymers by hydrogen-oxidizing bacteria
Место публикации : Applied Biochemistry and Microbiology. - 2011. - Vol. 47, Is. 5. - С. 494-499. - ISSN 00036838 (ISSN) , DOI 10.1134/S0003683811050152
Ключевые слова (''Своб.индексиров.''): bacteria (microorganisms)--cupriavidus--cupriavidus necator
Аннотация: Synthesis of 3- and 4-hydroxybutyrate copolymer (3HB-co-4HB), the most promising member of the biodegradable polyhydroxyalkanoate (PHA) family, has been studied. Cultivation conditions of naturally occurring strains of hydrogen-oxidizing bacteria Ralstonia eutropha B5786 and Cupriavidus eutrophus B10646 have been optimized to ensure efficient synthesis of the 3HB-co-4HB copolymer. A set of highly pure samples of the 3HB-co-4HB copolymer with 4HB content varying from 8.7 to 24.3 mol% has been obtained. Incorporation of 4HB into the copolymer was shown to cause a more pronounced decrease in polymer crystallinity than the incorporation of 3-hydroxyvalerate or 3-hydroxyhexanoate; samples with a degree of crystallinity below 30% have been obtained. The weight average molecular mass of the 3HB-co-4HB copolymers was shown to be independent on the monomer ratio and to vary broadly (from 540 to 1110 kDa). В© 2010 Pleiades Publishing, Ltd.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kiselev E. G., Vasiliev A. D., Volova T. G.
Заглавие : Synthesis and characterization of multicomponent PHAs
Место публикации : J. Sib. Fed. Univ. - Biol.: Siberian Federal University, 2021. - Vol. 14, Is. 1. - С. 97-113. - ISSN 19971389 (ISSN), DOI 10.17516/1997-1389-0325
Примечания : Cited By :1
Аннотация: Cupriavidus necator B10646 bacterial cells were cultivated in the mode of synthesis of the reserve polyhydroxyalkanoates (PHAs) in the growth medium that contained, in addition to glucose as the main substrate, precursor substrates of the monomers of various monocarboxylic acids - salts of valeric and hexanoic acids, propionate, and ?-butyrolactone. PHA terpolymers and quaterpolymers with different compositions and proportions of monomers were synthesized, and their physicochemical properties were studied. The terpolymers were composed of monomers of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), 4-hydroxybutyrate (4HB), or 3-hydroxyhexanoate (3HHx) and had the following compositions: P(3HB/3HB/4HHx) and P(3HB/3HV/4HHx). The quaterpolymers had the following composition: P(3HB/3HV/4HB/3HHx). All copolymer samples, regardless of the composition and proportions of monomers, had lower molecular weights and higher polydispersity values compared to the highly crystalline 3-hydroxybutyrate homopolymer, but retained thermal stability properties, with a difference between the melting point and thermal degradation of at least 100-110 °C. The inclusion of 3HV, 4HB, and 3HHx monomers in the C-chain of 3HB caused changes in the crystalline to amorphous phase ratio and a significant decrease in the degree of crystallinity (Cx), which depended on the type of monomers and their contents in the copolymer. The maximum decrease in Cx (9-17 %) was detected in the P(3HB/3HV/4HB) terpolymer and the P(3HB/3HV/4HB/3HHx) quaterpolymer (30-36 %). The study confirms that there is the possibility of synthesizing polymers with various compositions, including new ones, which differ significantly in their basic properties. © Siberian Federal University. All rights reserved.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Voinov N.A.
Заглавие : Study of Ralstonia eutropha culture producing polyhydroxyalkanoates on products of coal processing
Место публикации : Prikladnaia biokhimiia i mikrobiologiia. - 2004. - Vol. 40, Is. 3. - С. 296-300. - ISSN 05551099 (ISSN)
Ключевые слова (''Своб.индексиров.''): carbon monoxide--coal--polyester--antibiotic resistance--article--culture medium--enzyme specificity--gas--growth, development and aging--metabolism--microbiology--wautersia eutropha--carbon monoxide--coal--culture media--cupriavidus necator--drug resistance, microbial--gases--polyesters--substrate specificity
Аннотация: Kinetic indices of growth, polyhydroxyalkanoate (PHA) accumulation, and gas exchange have been studied in a culture of the carbon monoxide-resistant hydrogen strain Ralstonia eutropha B-5786 grown on a gaseous substrate (GS) obtained by lignite gasification. The GS was shown to be suitable for PHA production. To increase the degree of GS consumption, various modes of gas supply to the culture were tested. Based on the results, an algorithm was developed for calculating and controlling gas-exchange parameters in the PHA-accumulating culture of Ralstonia eutropha, grown on a new GS allowing high polymer yields (up to 75%) and degrees of the substrate utilization (up to 90%).
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Voinov N.A.
Заглавие : Study of a Ralstonia eutropha culture producing polyhydroxyalkanoates on products of coal processing
Место публикации : Applied Biochemistry and Microbiology. - 2004. - Vol. 40, Is. 3. - С. 249-252. - ISSN 00036838 (ISSN) , DOI 10.1023/B:ABIM.0000025946.47013.03
Ключевые слова (''Своб.индексиров.''): alkanoic acid--carbon monoxide--coal--hydrogen--lignite--algorithm--article--bacterial strain--bacterium culture--coal gasification--controlled study--gas--gas exchange--mathematical computing--nonhuman--ralstonia eutropha--bacteria (microorganisms)--ralstonia--wautersia eutropha
Аннотация: Kinetic indices of growth, polyhydroxyalkanoate (PHA) accumulation, and gas exchange were studied in a culture of the carbon monoxide-resistant hydrogen strain Ralstonia eutropha B-5786 grown on a gaseous substrate (GS) obtained by lignite gasification. The GS was shown to be suitable for PHA production. To increase the degree of GS consumption, various modes of gas supply to the culture were tested. Based on the results, an algorithm was developed for calculating and controlling gas-exchange parameters in the PHA-accumulating culture of Ralstonia eutropha, grown on a new GS allowing high polymer yields (up to 75%) and degrees of substrate utilization (up to 90%).
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Voinov N.A.
Заглавие : Study of a Ralstonia eutropha culture producing polyhydroxyalkanoates on products of coal processing
Колич.характеристики :4 с
Место публикации : Appl. Biochem. Microbiol.: MAIK NAUKA/INTERPERIODICA, 2004. - Vol. 40, Is. 3. - P249-252. - ISSN 0003-6838, DOI 10.1023/B:ABIM.0000025946.47013.03
Примечания : Cited References: 18
Аннотация: Kinetic indices of growth, polyhydroxyalkanoate (PHA) accumulation, and gas exchange were studied in a culture of the carbon monoxide-resistant hydrogen strain Ralstonia eutropha B-5786 grown on a gaseous substrate (GS) obtained by lignite gasification. The GS was shown to be suitable for PHA production. To increase the degree of GS consumption, various modes of gas supply to the culture were tested. Based on the results, an algorithm was developed for calculating and controlling gas-exchange parameters in the PHA-accumulating culture of Ralstonia eutropha, grown on a new GS allowing high polymer yields (up to 75%) and degrees of substrate utilization (up to 90%).
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Belyaeva O.G., Plotnikov V.F., Puzyr' A.P.
Заглавие : Studies of Biodegradation of Microbial Polyhydroxyalkanoates
Место публикации : Prikladnaya Biokhimiya i Mikrobiologiya. - 1998. - Vol. 34, Is. 5. - С. 543. - ISSN 05551099 (ISSN)
Аннотация: Biodegradation of polyhydroxyalkanoates (PHA) - polymers of hydroxylated fatty acids - differing in chemical composition, crystallinity, melting temperature, and molecular weight was studied in soil and water environments under conditions of varying ambient temperature and pH. Under similar conditions, homogeneous films of polyhydroxybutyrate (PHB) with different initial properties were degraded at virtually equal rates. Hydroxybutyrate-hydroxyvalerate copolymers with a lower crystallinity and different microstructure in comparison to PHB were degraded at a 20 to 30% higher rate on average. The ambient temperature significantly influenced the rate of PHA biodegradation.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Belyaeva O.G., Plotnikov V.F., Puzyr' A.P.
Заглавие : Studies of biodegradation of microbial polyhydroxyalkanoates
Место публикации : Applied Biochemistry and Microbiology. - 1998. - Vol. 34, Is. 5. - С. 488-492. - ISSN 00036838 (ISSN)
Аннотация: Biodegradation of polyhydroxyalkanoates (PHA) - polymers of hydroxylated fatty acids - differing in chemical composition, crystallinity, melting temperature, and molecular weight was studied in soil and water environments under conditions of varying ambient temperature and pH. Under similar conditions, homogeneous films of polyhydroxybutyrate (PHB) with different initial properties were degraded at virtually equal rates. Hydroxybutyrate-hydroxyvalerate copolymers with a lower crystallinity and different microstructure in comparison to PHB were degraded at a 20 to 30% higher rate on average. The ambient temperature significantly influenced the rate of PHA biodegradation.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prudnikova S. V., Vinogradova O. N., Trusova M. Y.
Заглавие : Specific character of bacterial biodegradation of polyhydroxyalkanoates with different chemical structure in soil
Место публикации : Doklad. Biochem. Biophys.: Maik Nauka Publishing / Springer SBM, 2017. - Vol. 473, Is. 1. - С. 94-97. - ISSN 16076729 (ISSN) , DOI 10.1134/S1607672917010185
Аннотация: The study addresses the influence of the physicochemical properties of the reserve cellular macromolecules (polyhydroxyalkanoates, PHA) with different chemical composition on their biodegradation in the agro-transformed field soil of the Siberian region (Krasnoyarsk Territory, Russia). It was shown that the degradation of the PHA samples depends on the degree of polymer crystallinity (Cx). For the first time, it was shown that the range of PHA-degrading microorganisms differs for each of PHA types. The study defines the primary degraders specific to each PHA type and common to all types of examined polymers. © 2017, Pleiades Publishing, Ltd.
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