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POPULATION-STRUCTURE DYNAMICS OF A RECOMBINANT STRAIN OF ESCHERICHIA-COLI IN CONTINUOUS CULTURE [Text] / L. Y. POPOVA [et al.] // Microbiology. - 1992. - Vol. 61, Is. 4. - P417-422. - Cited References: 10 . - ISSN 0026-2617

РУБ Microbiology

Аннотация: We studied the population structure of Escherichia coli strain Z905, carrying the recombinant plasmid of the Ap(r)Lux+ phenotype, in continuous culture in a chemostat. We found that the maintenance of a stable, defined ratio of plasmidless to plasmid-carrying cells in the population depended primarily on the presence in the medium of the selective factor (ampicillin), and also on the nature of the growth-limiting carbon and energy source.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
POPOVA, L.Y.; LUTSKAYA, N.I.; BOGUCHAROV, A.A.; BRILKOV, A.V.; PECHURKIN, N.S.

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Phenotypic variability of the population of a recombinant luminescent strain of Escherichia coli in aqueous microcosms [Text] / T. V. Kargatova [et al.] // Microbiology. - 1997. - Vol. 66, Is. 1. - P85-90. - Cited References: 12 . - ISSN 0026-2617

РУБ Microbiology
Рубрики:
GENETICALLY-MODIFIED MICROORGANISMS
Аннотация: The behavior of Escherichia coil Z905, carrying a recombinant plasmid pPHL7 with genes determining ampicillin resistance and bacterial luminescence, and the efficiency of expression of cloned genes were studied after introduction of the strain into model aqueous ecosystems with different trophic chain lengths. The E. coli Z905 variants isolated from ecosystems after different periods of time were found to vary in their resistance to ampicillin (from 50 to 0.05 mu g/ml) and in the intensity of bioluminescence. An increase in the concentration of the selective factor (ampicillin) or in the extent of the aqueous microcosm blooming restored the expression of the recombinant plasmid genes in some clones.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kargatova, T.V.; Maksimova, E.E.; Popova, L.Y.; Brilkov, A.V.; Pechurkin, N.S.

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Controlled expression of bacterial luminescence genes cloned in a multicopy recombinant plasmid [Text] / E. E. Maksimova [et al.] // Microbiology. - 1997. - Vol. 66, Is. 2. - P. 184-187. - Cited References: 17 . - ISSN 0026-2617

РУБ Microbiology
Рубрики:
GENETICALLY-MODIFIED MICROORGANISMS
BIOLUMINESCENCE
ENVIRONMENT
Кл.слова (ненормированные):
recombinant plasmid -- Escherichia coli -- luminescence -- catabolite repression
Аннотация: Luminescence and growth responses of the recombinant strain Escherichia coil Z905 (Ap(r)Lux(+)) to different concentrations of ampicillin depended on the source of carbon and energy. When glycerol was used as the substrate, the intensity of luminescence rose with the ampicillin concentration in the medium. Glucose caused catabolite repression of cell luminescence up to the late stationary phase, and ampicillin enhanced this effect. The feasibility of controlling the expression of cloned lux genes was shown.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Maksimova, E.E.; Popova, L.Y.; Kargatova, T.V.; Shpagina, V.V.

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A study on the possibility of environmental adaptation of a Bacillus subtilis strain containing a recombinant plasmid with the gene of human interferon alpha 2 [Text] / L. Y. Popova [et al.] // Microbiology. - 1997. - Vol. 66, Is. 6. - P. 637-641. - Cited References: 13 . - ISSN 0026-2617

РУБ Microbiology
Рубрики:
GENETICALLY-MODIFIED MICROORGANISMS
Кл.слова (ненормированные):
microecosystem -- Bacillus subtilis -- recombinant plasmid -- interferon -- adaptation
Аннотация: Adaptation of the Bacillus subtilis strain 2335/105 (Km(r)Inf(+)) containing a recombinant plasmid encoding the extracellular human interferon alpha 2 was studied under various conditions. Stability of the plasmid in the population of B. subtilis 2335/105 was estimated under nonselective conditions. The plasmid-free cells and cells with a low number of plasmid copies were found to accumulate progressively, constituting 80% of the population after 10 culture passages, indicating the poor competitiveness of cells carrying a high number of plasmid copies. The behavior of vegetative cells of the recombinant strain introduced into aquatic microcosms differing in trophic chain length was studied. Within the first 10 days, the lysis of vegetative cells of B. subtilis 2335/105 occurred; the number of viable spores was very low but remained constant for half a year.

WOS
Держатели документа:
Russian Acad Sci, Akademgorodok, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
State Sci Ctr VB Vektor, Sci Res Design & Technol Inst Biol Act Subst, Berdsk 633190, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Popova, L.Y.; Kargatova, T.V.; Maksimova, E.E.; Belyavskaya, V.A.

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Salt-dependent inhibition of light emitting of the luminescent microorganism Escherichia coli Z9051 [Текст] / A. N. Boyandin, L. Y. Popova // Biofizika. - 2001. - Vol. 46, Is. 2. - С. 251-255. - Cited References: 12 . - 5. - ISSN 0006-3029

РУБ Biophysics

Кл.слова (ненормированные):
bacterial luminescence -- recombinant plasmid -- salt concentration
Аннотация: The influence of some mineral salts on the recombinant strain Escherichia coli Z9051 was investigated. It was shown that the composition (NaCl, Na2SO4, MgCl2 and MgSO4) and concentration (5 and 10%) of the salts substantially affect the expression of genes for the luminescence system of fight-emitting bacteria cloned in the plasmid under the control of the lac-promoter. In some cases, the luminescence level of the microorganism in the presence of salts was similar to the luminescence level under catabolite repression by glucose, the more strong influence of the salts exceeding the effect of catabolite repression. The possibility of adaptation of the genetically modified microorganism to the salinity factor is discussed.

Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Popova, L.Y.

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Quantitative criteria for estimating the effectiveness of bioluminescence expression in natural and transgenic luminescent bacteria / A. A. Gusev [et al.] // Biophysics. - 2008. - Vol. 53, Is. 5. - P417-420, DOI 10.1134/S0006350908050175 . - ISSN 0006-3509
Кл.слова (ненормированные):
Effectiveness of lux-operon expression -- Natural and transgenic bacteria -- Bacteria (microorganisms) -- Escherichia coli -- Photobacterium leiognathi
Аннотация: Computational coefficients for estimating the effectiveness of bioluminescence expression in natural luminescent bacteria Photobacterium leiognathi 54 and transgenic strain E. coli Z905/pPHL7 bearing lux-operon in a multicopy plasmid are suggested and their use at molecular, cell, and population levels was considered. It was shown that at the population level, all transgenic variants have an advantage over natural variants of P. leiognathi 54 irrespective of the type of lux-operon regulation. At the cell level, the effectiveness of bioluminescence expression in the bright and dim variants of the transgenic strain increased by several orders. At the level of one lux-operon, the effectiveness of expression in the bright variant of the transgenic strain is substantially higher than in the natural bright variant; in dim variants, the efficiency values are similar; the effectiveness of bioluminescence expression in the dark variant of E. coli Z905-2/pPHL7 is by two orders of magnitude lower than in the dark variant of P. leiognathi 54. В© 2008 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50/50, Krasnoyarsk, 660036, Russian Federation
Krasnoyarsk Scientific Center, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gusev, A.A.; Kargatova, T.V.; Medvedeva, S.E.; Popova, L.Yu.

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The interaction of linear and ring forms of DNA molecules with nanodiamonds synthesized by detonation [Text] / K. V. Purtov [et al.] // Nanotechnology. - 2008. - Vol. 19, Is. 32. - Ст. 325101, DOI 10.1088/0957-4484/19/32/325101. - Cited References: 13 . - ISSN 0957-4484

РУБ Nanoscience & Nanotechnology + Materials Science, Multidisciplinary + Physics, Applied
Рубрики:
DIAMOND
NANOPARTICLES
Аннотация: Nanodiamonds synthesized by detonation have been found not to immobilize the ring form of pUC19 plasmid DNA. Linear pUC19 molecules with blunt ends, prepared by restriction of the initial ring form of pUC19 DNA, and linear 0.25-10 kb DNA fragments are adsorbed on nanodiamonds. The amount of adsorbed linear DNA molecules depends on the size of the molecules and the size of the nanodiamond clusters.

Держатели документа:
[Purtov, K. V.
Burakova, L. P.
Puzyr, A. P.
Bondar, V. S.] Inst Biophys SB RAS, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Purtov, K.V.; Burakova, L.P.; Puzyr, A.P.; Bondar, V.S.

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SEQUENCE OF THE CDNA-ENCODING THE CA2+-ACTIVATED PHOTOPROTEIN OBELIN FROM THE HYDROID POLYP OBELIA-LONGISSIMA [Text] / B. A. ILLARIONOV [et al.] // Gene. - 1995. - Vol. 153, Is. 2. - P273-274, DOI 10.1016/0378-1119(94)00797-V. - Cited References: 6 . - 2. - ISSN 0378-1119

РУБ Genetics & Heredity
Рубрики:
CA-2+-ACTIVATED PHOTOPROTEIN
AEQUORIN
CLONING
Кл.слова (ненормированные):
BIOLUMINESCENCE -- CALCIUM -- GENE -- PLASMID -- MARINE COELENTERATES
Аннотация: A cDNA clone encoding the Ca2+-activated photoprotein, obelin (Obl), from Obelia longissima was sequenced. The nucleotide (nt) sequence contained two long overlapping open reading frames (ORFs), one of which encoded apoobelin (apoObl). The deduced amino acid (aa) sequence of apoObl revealed that this 195-aa protein has three EF-hand structures that are characteristic for Ca2+-binding domains. Strong aa homology was shown among apoObl, apoaequorin and apoclytin. The second ORF present in the obl cDNA consists of 139 codons and encodes a very basic protein with a calculated pI of 10.56 and a molecular mass of 16153 Da.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
ILLARIONOV, B.A.; BONDAR, V.S.; ILLARIONOVA, V.A.; VYSOTSKI, E.S.

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Salt-dependent inhibition of light emitting of the luminescent microorganism Escherichia coli Z9051 [Текст] / A. N. Boyandin, L. Y. Popova // Biofizika. - 2001. - Vol. 46, Is. 2. - P. 251-255. - Cited References: 12 . - ISSN 0006-3029

РУБ Biophysics

Кл.слова (ненормированные):
bacterial luminescence -- recombinant plasmid -- salt concentration
Аннотация: The influence of some mineral salts on the recombinant strain Escherichia coli Z9051 was investigated. It was shown that the composition (NaCl, Na2SO4, MgCl2 and MgSO4) and concentration (5 and 10%) of the salts substantially affect the expression of genes for the luminescence system of fight-emitting bacteria cloned in the plasmid under the control of the lac-promoter. In some cases, the luminescence level of the microorganism in the presence of salts was similar to the luminescence level under catabolite repression by glucose, the more strong influence of the salts exceeding the effect of catabolite repression. The possibility of adaptation of the genetically modified microorganism to the salinity factor is discussed.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Popova, L.Y.

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10.

The influence of salt concentration on the copy number of plasmid pSH1 replicating in Micrococcus sp 9 [Text] / T. I. Lobova, S. N. Zagrebel'nyi, L. Y. Popova // Microbiology. - 2005. - Vol. 74, Is. 3. - P. 296-302, DOI 10.1007/s11021-005-0066-z. - Cited References: 19 . - ISSN 0026-2617

РУБ Microbiology
Рубрики:
CYANOBACTERIUM-SYNECHOCOCCUS SP
ENDOGENOUS PLASMID
Кл.слова (ненормированные):
saline lakes -- halotolerance -- heterotrophic bacteria -- plasmids
Аннотация: The study of heterotrophic bacteria isolated from the brackish waters of Lake Shira has shown that some of them contain plasmid pSH1 of approximately 2.7 kb in size. The number of plasmid copies in plasmid-containing strains cultivated at a minimal concentration of sodium chloride is found to be low, whereas the sub-culturing of these strains at high salt concentrations increases the plasmid number. The role of natural pSH1 plasmid in the osmotolerance of host bacteria is discussed.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
Novosibirsk State Univ, Novosibirsk 630090, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Lobova, T.I.; Zagrebel'nyi, S.N.; Popova, L.Y.

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11.

Experimental evaluation of the processes resulting from the introduction of the transgenic microorganism Escherichia coli Z905/pPHL7 (luk(+)) into aquatic microcosms [Text] / T. V. Kargatova [et al.] ; ed. M Nelson [et al.] // SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - P. 1769-1774, DOI 10.1016/S0273-1177(03)00119-4. - Cited References: 16 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Ecology + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences
Рубрики:
SURVIVAL
PROTEIN
Аннотация: The processes resulting from the introduction of the transgenic microorganism (TM) E. coli Z905/pPHL7 into aquatic microcosms have been modeled experimentally. It has been shown that the TM E. coli is able to adapt to a long co-existence with indigenous heterotrophic microflora in variously structured microcosms. In more complex microcosms the numerical dynamics of the introduced E. coli Z905/pPHL7 population is more stable. In the TM populations staying in the microcosms for a prolonged time, changes are recorded in the phenotypic expression of plasmid genes (ampicillin resistance and the luminescence level) and chromosome genes (morphological and physiological traits). However, in our study microcosms, the recombinant plasmid persisted in the TM cells for 6 years after die introduction, and as the population adapts to the conditions of the microcosms, the efficiency of the cloned gene expression in the cells is restored. In the microcosms with high microalgal counts (10(7) cells/ml), cells with a high threshold of sensitivity to ampicillin dominate in the population of the TM E. coli Z905/pPHL7. (C) 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
SB RAS, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kargatova, T.V.; Boyandin, A.N.; Popova, L.Y.; Pechurkin, N.S.; Nelson, M \ed.\; Pechurkin, NS \ed.\; Dempster, WF \ed.\; Somova, LA \ed.\; Somo, , LA \ed.\

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12.

Development of the method to produce functionally active recombinant streptavidin in escherichia coli cells / E. E. Bashmakova, A. N. Kudryavtsev, L. A. Frank // J. Sib. Fed. Univ. - Biol. - 2020. - Vol. 13, Is. 2. - С. 218-229, DOI 10.17516/1997-1389-0324 . - ISSN 1997-1389
Кл.слова (ненормированные):
E. coli protein-producing strain -- Microanalysis -- Recombinant streptavidin
Аннотация: Streptavidin is a homotetrameric protein produced by Streptomyces avidinii, each subunit of which binds biotin (vitamin H), forming a stable complex (Kd = 10-15 M). Streptavidin-biotin coreaction is widely used in analytical systems, for targeted delivery of compounds, for affinity purification, etc. The aim of this study was to develop a rational technique to produce functionally active recombinant streptavidin. Recombinant Escherichia coli strains producing minimal core and full-sized streptavidin variants were obtained. The E. coli BL21 Codon Plus (DE3) RIPL, as host cells, and the pET19b plasmid carrying gene of minimally-sized core (miniSAV) or full-sized (SAV) streptavidin were used. Synthesis of miniSAV results in its localization as insoluble inclusion bodies. Denatured miniSAV yield was 130 mg per liter of E. coli c ulture. T he r enaturation g ives o nly 10- 15 % of the functionally active protein. Full-sized streptavidin localizes in the cytoplasm in a soluble state, but its toxicity causes low yield of the protein (10-13 mg per liter of the culture). The induction of SAV synthesis at the end of the logarithmic stage of cell growth was found to increase the yield of SAV approximately 2-fold. The yield of functionally active protein was 30 mg per liter culture. SAV was produced practically in individual state after affine chromatography on 2-iminobiotin agarose. One molecule of full-sized streptavidin bound 3.9 biotin molecules as was shown by colorimetric analysis using HABA (4-hydroxyazobenzene-2-carboxylic acid). Both streptavidins form sandwichtype complexes with biotinylated molecules in solid-phase microassay conditions. E. coli BL21 Codon Plus (DE3) RIPL/pET19bSAV strain was stable during storage with 20 % glycerol at -70 °C, which was shown by repeated two-year reseeding. The streptavidin producing strain (E. coli BL21 Codon Plus (DE3) RIPL/pET19bSAV) is deposited in the Collection for extremophile microorganisms and type cultures (Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk), No. 3505. The method for producing functionally active recombinant streptavidin developed in this study ensures its availability for biotechnological research. © Siberian Federal University. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, FRC Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Bashmakova, E. E.; Kudryavtsev, A. N.; Frank, L. A.

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13.

Formation of structured communities by natural and transgenic naphthalene-degrading bacteria [Text] / O. A. Mogil'naya [et al.] // Appl. Biochem. Microbiol. - 2005. - Vol. 41, Is. 1. - P. 63-68, DOI 10.1007/s10438-005-0012-x. - Cited References: 18 . - ISSN 0003-6838

РУБ Biotechnology & Applied Microbiology + Microbiology
Рубрики:
GENETICALLY-ENGINEERED MICROORGANISM
   POLYCYCLIC AROMATIC-HYDROCARBONS
   BIOFILM FORMATION
   DEGRADATION
Аннотация: This study concerns the formation of structured communities by monocultures and binary associations of Pseudomonas fluorescens transgenic strains and natural heterotrophic bacterial species in naphthalene-containing media with various osmotic pressures. It was shown that cells of P. fluorescens strain 5RL, harboring a recombinant construct in the chromosome, were more resistant to the combined action of the stress factors under study than P. fluorescens 82/pUTK21, harboring a recombinant construct within a plasmid. Natural P. fluorescens 1 strains, particularly Vibrio sp. 14, were more viable at high osmotic pressures and naphthalene concentrations. Experiments with the combined introduction of transgenic and natural bacterial strains at high osmotic pressures demonstrated the stable coexistence of bacterial associations in biofilms, independent of naphthalene concentration. Strains considered for introduction into the environment for bioremediation should be assessed with regard to their susceptibility to the combined effect of anthropogenic and natural stress factors. The design of bacterial associations for the same purpose should take into account the effect of factors important for their survival in polluted areas.

WOS
Держатели документа:
Russian Acad Sci, Siberian Div, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Mogil'naya, O.A.; Krivomazova, E.S.; Kargatova, T.V.; Lobova, T.I.; Popova, L.Y.

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14.

Population dynamics of transgenic microorganisms in the different microecosystem conditions [Text] / L. Y. Popova [et al.] ; ed. , AN Boyand // SPACE LIFE SCIENCES: CLOSED ECOLOGICAL SYSTEMS: EARTH AND SPACE APPLICATIONS. Ser. ADVANCES IN SPACE RESEARCH : ELSEVIER SCIENCE BV, 2001. - Vol. 27: F4 4 Symposium of COSPAR Scientific Commission F held at the 33rd COSPAR Scientific Assembly (JUL, 2000, WARSAW, POLAND), Is. 9. - P. 1571-1579, DOI 10.1016/S0273-1177(01)00248-4. - Cited References: 8 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences
Рубрики:
GENETICALLY-ENGINEERED MICROORGANISMS
Аннотация: The role of key environmental factors in adaptation of spore-forming and non-spore-forming transgenic microorganisms (TM) have been studied in model ecosystems. Model TM Escherichia coli Z905 (bearing plasmid genes of bacterial luminescence Ap(r)Lux(+)) has been found to have a higher adaptation potential than TM Bacillus subtilis 2335/105 (bearing genes of human alpha 2-interferon Km(r)lnf(+)), planned for employment as a living vaccine under varying environmental conditions. Effects of abiotic factors on migration of natural and recombinant plasmids between microorganisms under model ecosystem conditions has been estimated. The transgenic microorganisms with low copy number survived better under introduction conditions in the microcosms studied. This trend has been shown to be independent of the microcosm type and its complexity. (C) 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Popova, L.Y.; Lobova, T.I.; Krylova, T.Y.; Kargatova, T.V.; Maksimova, E.E.; Boyandin, A.N.; Pechurkin, N.S.; Boyand, , AN \ed.\

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15.

Coexistence of genetically engineered Escherichia coli strains and natural microorganisms in experimental aquatic microcosms [Text] / T. V. Kargatova, E. E. Maksimova, L. Y. Popova // Microbiology. - 2001. - Vol. 70, Is. 2. - P. 211-216, DOI 10.1023/A:1010437731920. - Cited References: 17 . - ISSN 0026-2617

РУБ Microbiology
Рубрики:
SURVIVAL
BACTERIA
GROWTH
Кл.слова (ненормированные):
Escherichia coli -- aquatic microecosystems -- recombinant plasmid
Аннотация: In experimental aquatic microcosms (AMCs), the population of the Escherichia coli strain Z905 harboring the recombinant plasmid pPHL7 (Ap(r)Lux(+)) was found to gradually accumulate AMC-adapted cells, which retained the plasmid but differed from the original cells in some biochemical and physiological characteristics. Both the original and the AMC-adapted E. coil cells could coexist with the native AMC microflora for one year or longer. When introduced into AMCs together with native pseudomonads, the AMC-adapted E. coil Z905-33 (pPHL7) cells were more competitive than the nonadapted cells.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kargatova, T.V.; Maksimova, E.E.; Popova, L.Y.

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16.

Survival and alteration of the plasmid-containing microorganism Escherichia coli Z905/pPHL7 introduced into manmade closed aquatic microcosms [Text] / A. N. Boyandin [et al.] ; ed. M Nelson [et al.] // SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - P. 1763-1768, DOI 10.1016/S0273-1177(03)00118-2. - Cited References: 9 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Ecology + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences
Рубрики:
MARKER
SOIL
Аннотация: It has been demonstrated that the transgenic microorganism Escherichia coli Z905/pPHL7 (Ap(r)Lux(+)) can exist for a long time at an elevated concentration of mineral salts. The microorganism was introduced into microcosms with sterile brackish water (salinity variable from 21 to 22 g l(-1)) taken from Lake Shira (Khakasia, Russia). The survival of the microorganism was estimated both by measuring the growth of the colonies on solid nutrient media and by the bioluminescence exhibited by the transgenic strain in samples from the microcosms and in the enrichment culture with the added selective factor - ampicillin (50 mug/ml). In the enrichment culture, the bioluminescent signal was registered through the 160-day experiment. It has been shown that in the closed microcosms with brackish water the E. coli strain becomes heterogeneous in its ampicillin resistance. The wpopulations of the transgenic strain were mainly represented by isolates able to persist in the medium containing 50 mug/ml, but there were also the cells (about 10%) with the threshold of ampicillin resistance not more than 0.05 mug/ml. Thus, it was shown that in the microcosms with brackish water and in the absence of the selective factor the transgenic strain survives and retains the recombinant plasmid. (C) 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Lobova, T.I.; Popova, L.Y.; Pechurkin, PNS; Nelson, M \ed.\; Pechurkin, NS \ed.\; Dempster, WF \ed.\; Somova, LA \ed.\; Somo, , LA \ed.\

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17.

Expression of cloned genes of transgenic microorganisms introduced into man-made ecosystems [Text] / E. E. Maksimova, L. Y. Popova ; ed. a, EE Maksim // SPACE LIFE SCIENCES: CLOSED ECOLOGICAL SYSTEMS: EARTH AND SPACE APPLICATIONS. Ser. ADVANCES IN SPACE RESEARCH : ELSEVIER SCIENCE BV, 2001. - Vol. 27: F4 4 Symposium of COSPAR Scientific Commission F held at the 33rd COSPAR Scientific Assembly (JUL, 2000, WARSAW, POLAND), Is. 9. - P. 1581-1586, DOI 10.1016/S0273-1177(01)00249-6. - Cited References: 17 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences
Рубрики:
MICROCOSMS
BACTERIA
Аннотация: Modeling of transgenic microorganism introduction into small man-made ecosystems can help forecast changes in expression of cloned genes under different conditions of existence. Introduction of the E. coli Z905/pPHL7 strain containing a plasmid with luminescent system genes of luminous bacteria led to changes in cell and colony morphology, reduction in metabolic activity of cells, and, as a result, a lower level of expression of cloned gene. A low concentration of nutrients has been shown to favor greatly the phenotypic change of cells of the recombinant strain. Expression of cloned genes changed due to: a lower concentration of plasmid DNA, a change in regulation of cloned genes, and a change in cells of biosynthesis of substrates needed for expression of luminescent genes. The conducted investigations can provide a basis for the use of marker transgenic microorganisms in closed ecosystems of different types. (C) 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Maksimova, E.E.; Popova, L.Y.; Maksim, a, EE \ed.\

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18.

^a621.37.99^2VINITI
Д 46

Динамика структуры популяции рекомбинантного штамма Escherichia coli при непрерывном культивировании [Текст] : научное издание / Л. Ю. Попова [и др.] // Микробиология. - 1992. - Т. 61, N 4. - С. 598-603 . - ISSN 0026-3656

ГРНТИ

62.37.99

РУБ 621.37.99
Рубрики:
КУЛЬТИВИРОВАНИЕНЕПРЕРЫВНОЕ
   ESCHERICHIACOLI
   РЕКОМБИНАНТНЫЙШТАММ
   ПЛАЗМИДА
   УСЛОВИЯСОХРАНЕНИЯВПОПУЛЯЦИИКЛЕТОК
   CONTINUOUSCULTURE
   PLASMID
Аннотация: Исследовали структуры популяции штамма Escherichia coli Z905, несущего рекомбинантную плазмиду с фенотипом Ap{r} ux{+}, при непрерывном культивировании в хемостате. Показано, что устойчивое поддержание определенного соотношения числа бесплазмидных и плазмидных клеток в популяции зависит прежде всего от наличия в среде селективного фактора (ампициллина), а также от типа источника углерода и энергии, лимитирующего рост. СНГ, Ин-т биофизики СО РАН, Красноярск. Библ. 10
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Попова, Л.Ю.; Луцкая, Н.И.; Богучарова, А.А.; Брильков, А.В.; Печуркин, Н.С.

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19.

Population heterogeneity of plasmid-bearing and plasmid-free Bacillus subtilis strains under different environmental conditions / T. Yu. Krylova [et al.] // Microbiology. - 2000. - Vol. 69, Is. 2. - P220-224 . - ISSN 0026-2617
Кл.слова (ненормированные):
Bacillus subtilis -- Heterogeneity -- Introduction -- Microcosm -- Recombinant plasmid
Аннотация: The population heterogeneity of recombinant and plasmid-free Bacillus subtilis strains introduced into aquatic microcosms was studied. After introduction, the population of the plasmid-free strain B. subtilis 2335 in microcosms has long been represented by both vegetative cells and spores, whereas, already ten days after introduction, the population of the recombinant strain B. subtilis 2335/105 (KmI)Inf+) was represented only by spores. The number of plasmid copies in the spore isolates of the recombinant strain was the same as before introduction, but the plasmid abundance in the vegetative isolates of this strain decreased. The isolates of B. subtilis 2335/105 obtained from microcosms and the variants of this strain obtained by ten successive subcultures on M9 and 0.1?M9 media with and without kanamycin (Km) differed in the number of plasmid copies, Km resistance, and maximum biomass yield during batch cultivation. Irrespective of the presence of Km, more than 50% of the variants subcultured on M9 medium showed reduced plasmid abundance. At the same time, about 70% of the variants subcultured on 0.1 ? M9 medium with Km and 90% of the variants subcultured on the same medium without Km retained the initial number of plasmid copies. The variants subcultured on media with Km retained the initial biomass level. In more than 70% of the variants isolated from media without Km, the biomass yield increased. В© 2000 MAIK "Nauka/Interperiodica".

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Krylova, T.Yu.; Popova, L.Yu.; Pechurkin, N.S.; Kashperova, T.A.; Belyavskaya, V.A.

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20.

Effect of salinity on the adaptive capacity of recombinant strains of Escherichia coli and Bacillus subtilis / A. N. Boyandin [et al.] // Microbiology. - 2000. - Vol. 69, Is. 2. - P196-199 . - ISSN 0026-2617
Кл.слова (ненормированные):
Genetically modified microorganisms -- Salinity -- Salt resistance
Аннотация: The effect of different concentrations of salts on natural and recombinant strains of Bacillus subtilis and Escherichia coli was studied. The recombinant strain of B. subtilis was found to be more osmotolerant than the wild-type strain of this bacterium, whereas the opposite situation was observed for the recombinant and wild-type strains of E. coli. Some salts exerted a bacteriostatic effect on E. coli and B. subtilis. The adaptive capacity of recombinant strains depended on the number of plasmid copies in the cells. The introduction of recombinant bacteria into model ecosystems resulted in the generation of their variants with increased osmotolerance. В© 2000 MAIK "Nauka/Interperiodica".

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Lobova, T.I.; Krylova, T.Yu.; Kargatova, T.V.; Popova, L.Yu.; Pechurkin, N.S.

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