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Вид документа : Однотомное издание
Шифр издания : Е041/М 54
Автор(ы) : Мазин, Александр Владимирович, Кузнеделов К. Д., Краев А. С., Холодилов Н. Г.
Заглавие : Методы молекулярной генетики и генной инженерии : научное издание
Выходные данные : Новосибирск: Наука. Сиб. отд-ние, 1990
Колич.характеристики :247 с.
Коллективы : АН СССР. Сибирское отделение, Институт цитологии и генетики (Новосибирск)
Примечания : Библиогр. в конце глав. - Авт. указаны на обороте тит. л.
ISBN, Цена 5-02-029565-5: 3.10, 3.10, р.
ГРНТИ : 34.15.27 + 34.15.23 + 34.23.19
УДК : 577.21.08
ББК : Е041.10в7 + Е041.15в7
Предметные рубрики: Молекулярная генетика
Генная инженерия
Аннотация: Выделение ДНК... 7 Эндонуклеазы рестрикции II типа: свойства иприменение... 14 Введение метки в ДНК... 25 Блот-гибридизация ДНК накапроновых мембранах... 35 Трансформация E. coli плазмидной ДНК... 39Коннекторный способ клонирования кДНК... 44 Получение геномныхбиблиотек в -векторах... 56 Детекция рекомбинантной ДНК методоммолекулярной гибридизации... 74 Белоксинтезирующие системы in vitro иin vivo... 80 Направленный мутагенез in vitro. Индукция транзиций GC AT... 91 Метод килосеквенирования... 99 Определение нуклеотиднойпоследовательности ДНК методом Максама-Гилберта... 107 Клонирование исеквенирование в М13... 115 Анализ функциональных сайтов в геномахпро- и эукариот... 154DNA separation... 7 Type II restriction endonuclease:properties and application... 14 DNA labelling... 25 DNA blothybridization on kapron membranes... 35 E. coli plasmid DNAtransformation... 39 Connection method of cDNA cloning... 44Producing genomic libraries in lambda-vectors... 56 Recombinant DNAdetection by molecular hybridization technique... 74 Proteinsynthesizing systems in vitro and in vivo... 80 Directed mutagenesisin vitro GC AT transition induction... 91 Kilosequencing methods...99 Detection of DNA nucleotide sequence by Maxam and Gilbertmethod... 107 Cloning and sequencing in M13... 115 Analysis offunctional sites in genomes of pro- and eucells... 154
Экземпляры :КФ(1)
Свободны : КФ(1)
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Illarrionov B.A., Blinov V.M., Douchenko A.P., Protopopova M.V., Karginov V.A., Mertvetsov N.P., Gitelson J.I.
Заглавие : Isolation of bioluminescent functions from Photobacterium leiognathi: analysis of luxA, luxB, luxG and neighboring genes
Место публикации : Gene. - 1990. - Vol. 86, Is. 1. - С. 89-94. - ISSN 03781119 (ISSN)
Ключевые слова (''Своб.индексиров.''): bioluminescence--expression in e. coli--luciferase--molecular evolution--nucleotide sequence--protein alignment--recombinant dna--luciferase--amino acid sequence--article--bioluminescence--fungus--gene structure--genetic engineering--heredity--nonhuman--nucleotide sequence--priority journal--vibrionaceae--acyltransferases--amino acid sequence--bacterial proteins--base sequence--cloning, molecular--dna, bacterial--genes, structural, bacterial--luciferase--luminescence--molecular sequence data--operon--photobacterium--restriction mapping--escherichia coli--fungi--photobacterium leiognathi--vibrio harveyi--vibrionaceae
Аннотация: Genes encoding luminescence of Photobacterium leiognathi have been cloned in Escherichia coli. The luminescent clones were readily apparent. Among them, a clone containing a recombinant plasmid with a 13.5-kb insertion was identified. This DNA fragment contained all of the luminescence-encoding genes. The luciferase-encoding genes (lux) in this DNA fragment were localized. We have sequenced a part of the cloned lux region and identified the luxA, luxB and luxG genes encoding the ? and ? subunits of luciferase and a ? protein with an Mr of 26 180, respectively. The analysis of deduced amino acid sequences and comparison with known luciferase sequences from Vibrio harveyi, indicate the common origin of these proteins. В© 1990.
Scopus
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Попова Л.Ю., Луцкая Н.И., Богучарова А.А., Брильков А.В., Печуркин Н.С.
Заглавие : Динамика структуры популяции рекомбинантного штамма Escherichia coli при непрерывном культивировании : научное издание
Место публикации : Микробиология. - 1992. - Т. 61, N 4. - С. 598-603. - ISSN 0026-3656
ГРНТИ : 62.37.99
Предметные рубрики: КУЛЬТИВИРОВАНИЕНЕПРЕРЫВНОЕ
ESCHERICHIACOLI
РЕКОМБИНАНТНЫЙШТАММ
ПЛАЗМИДА
УСЛОВИЯСОХРАНЕНИЯВПОПУЛЯЦИИКЛЕТОК
CONTINUOUSCULTURE
PLASMID
Аннотация: Исследовали структуры популяции штамма Escherichia coli Z905, несущего рекомбинантную плазмиду с фенотипом Ap{r} ux{+}, при непрерывном культивировании в хемостате. Показано, что устойчивое поддержание определенного соотношения числа бесплазмидных и плазмидных клеток в популяции зависит прежде всего от наличия в среде селективного фактора (ампициллина), а также от типа источника углерода и энергии, лимитирующего рост. СНГ, Ин-т биофизики СО РАН, Красноярск. Библ. 10
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : POPOVA L.Y., LUTSKAYA N.I., BOGUCHAROV A.A., BRILKOV A.V., PECHURKIN N.S.
Заглавие : POPULATION-STRUCTURE DYNAMICS OF A RECOMBINANT STRAIN OF ESCHERICHIA-COLI IN CONTINUOUS CULTURE
Колич.характеристики :6 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1992. - Vol. 61, Is. 4. - С. 417-422. - ISSN 0026-2617
Примечания : Cited References: 10
Аннотация: We studied the population structure of Escherichia coli strain Z905, carrying the recombinant plasmid of the Ap(r)Lux+ phenotype, in continuous culture in a chemostat. We found that the maintenance of a stable, defined ratio of plasmidless to plasmid-carrying cells in the population depended primarily on the presence in the medium of the selective factor (ampicillin), and also on the nature of the growth-limiting carbon and energy source.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : ILLARIONOV B.A., BONDAR V.S., ILLARIONOVA V.A., VYSOTSKI E.S.
Заглавие : SEQUENCE OF THE CDNA-ENCODING THE CA2+-ACTIVATED PHOTOPROTEIN OBELIN FROM THE HYDROID POLYP OBELIA-LONGISSIMA
Место публикации : Gene: ELSEVIER SCIENCE BV, 1995. - Vol. 153, Is. 2. - С. 273-274. - 2. - ISSN 0378-1119, DOI 10.1016/0378-1119(94)00797-V
Примечания : Cited References: 6
Предметные рубрики: CA-2+-ACTIVATED PHOTOPROTEIN
AEQUORIN
CLONING
Ключевые слова (''Своб.индексиров.''): bioluminescence--calcium--gene--plasmid--marine coelenterates
Аннотация: A cDNA clone encoding the Ca2+-activated photoprotein, obelin (Obl), from Obelia longissima was sequenced. The nucleotide (nt) sequence contained two long overlapping open reading frames (ORFs), one of which encoded apoobelin (apoObl). The deduced amino acid (aa) sequence of apoObl revealed that this 195-aa protein has three EF-hand structures that are characteristic for Ca2+-binding domains. Strong aa homology was shown among apoObl, apoaequorin and apoclytin. The second ORF present in the obl cDNA consists of 139 codons and encodes a very basic protein with a calculated pI of 10.56 and a molecular mass of 16153 Da.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kargatova T.V., Maksimova E.E., Popova L.Yu., Bril'kov A.V., Pechurkin N.S.
Заглавие : Phenotypic variability of the population of a recombinant luminescent strain of escherichia coli in aqueous microcosms
Место публикации : Microbiology. - 1997. - Vol. 66, Is. 1. - С. 85-90. - ISSN 00262617 (ISSN)
Аннотация: The behavior of Escherichia coli Z905, carrying a recombinant plasmid pPHL7 with genes determining ampicillin resistance and bacterial luminescence, and the efficiency of expression of cloned genes were studied after introduction of the strain into model aqueous ecosystems with different trophic chain lengths. The E. coli Z905 variants isolated from ecosystems after different periods of time were found to vary in their resistance to ampicillin (from 50 to 0.05 ?g/ml) and in the intensity of bioluminescence. An increase in the concentration of the selective factor (ampicillin) or in the extent of the aqueous microcosm blooming restored the expression of the recombinant plasmid genes in some clones. В© 1997 MAHK Hayka/Interperiodica Publishing.
Scopus
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kargatova T.V., Maksimova E.E., Popova L.Yu., Bril'Kov A.V., Pechurkin N.S.
Заглавие : Phenotypic variability of the population of a recombinant luminescent strain of escherichia coli in aqueous microcosms
Место публикации : Mikrobiologiya. - 1997. - Vol. 66, Is. 1. - С. 101-106. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): article--escherichia coli--genetic recombination--genetics--luminescence--microbiology--penicillin resistance--phenotype--plasmid--ampicillin resistance--escherichia coli--luminescent measurements--phenotype--plasmids--recombination, genetic--water microbiology
Аннотация: The behavior of Escherichia coli Z905, carrying a recombinant plasmid pPHL7 with genes determining ampicillin resistance and bacterial luminescence, and the efficiency of expression of cloned genes were studied after introduction of the strain into model aqueous ecosystems with different trophic chain lengths. The E. coli Z905 variants isolated from ecosystems after different periods of time were found to vary in their resistance to ampicillin (from 50 to 0.05 ?g/ml) and in the intensity of bioluminescence. An increase in the concentration of the selective factor (ampicillin) or in the extent of the aqueous microcosm blooming restored the expression of the recombinant plasmid genes in some clones.
Scopus
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Popova L.Y., Kargatova T.V., Maksimova E.E., Belyavskaya V.A.
Заглавие : A study on the possibility of environmental adaptation of a Bacillus subtilis strain containing a recombinant plasmid with the gene of human interferon alpha 2
Колич.характеристики :5 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1997. - Vol. 66, Is. 6. - P637-641. - ISSN 0026-2617
Примечания : Cited References: 13
Предметные рубрики: GENETICALLY-MODIFIED MICROORGANISMS
Ключевые слова (''Своб.индексиров.''): microecosystem--bacillus subtilis--recombinant plasmid--interferon--adaptation
Аннотация: Adaptation of the Bacillus subtilis strain 2335/105 (Km(r)Inf(+)) containing a recombinant plasmid encoding the extracellular human interferon alpha 2 was studied under various conditions. Stability of the plasmid in the population of B. subtilis 2335/105 was estimated under nonselective conditions. The plasmid-free cells and cells with a low number of plasmid copies were found to accumulate progressively, constituting 80% of the population after 10 culture passages, indicating the poor competitiveness of cells carrying a high number of plasmid copies. The behavior of vegetative cells of the recombinant strain introduced into aquatic microcosms differing in trophic chain length was studied. Within the first 10 days, the lysis of vegetative cells of B. subtilis 2335/105 occurred; the number of viable spores was very low but remained constant for half a year.
WOS
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kargatova T.V., Maksimova E.E., Popova L.Y., Brilkov A.V., Pechurkin N.S.
Заглавие : Phenotypic variability of the population of a recombinant luminescent strain of Escherichia coli in aqueous microcosms
Колич.характеристики :6 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1997. - Vol. 66, Is. 1. - С. 85-90. - ISSN 0026-2617
Примечания : Cited References: 12
Предметные рубрики: GENETICALLY-MODIFIED MICROORGANISMS
Аннотация: The behavior of Escherichia coil Z905, carrying a recombinant plasmid pPHL7 with genes determining ampicillin resistance and bacterial luminescence, and the efficiency of expression of cloned genes were studied after introduction of the strain into model aqueous ecosystems with different trophic chain lengths. The E. coli Z905 variants isolated from ecosystems after different periods of time were found to vary in their resistance to ampicillin (from 50 to 0.05 mu g/ml) and in the intensity of bioluminescence. An increase in the concentration of the selective factor (ampicillin) or in the extent of the aqueous microcosm blooming restored the expression of the recombinant plasmid genes in some clones.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Maksimova E.E., Popova L.Y., Kargatova T.V., Shpagina V.V.
Заглавие : Controlled expression of bacterial luminescence genes cloned in a multicopy recombinant plasmid
Колич.характеристики :4 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1997. - Vol. 66, Is. 2. - P184-187. - ISSN 0026-2617
Примечания : Cited References: 17
Предметные рубрики: GENETICALLY-MODIFIED MICROORGANISMS
BIOLUMINESCENCE
ENVIRONMENT
Ключевые слова (''Своб.индексиров.''): recombinant plasmid--escherichia coli--luminescence--catabolite repression
Аннотация: Luminescence and growth responses of the recombinant strain Escherichia coil Z905 (Ap(r)Lux(+)) to different concentrations of ampicillin depended on the source of carbon and energy. When glycerol was used as the substrate, the intensity of luminescence rose with the ampicillin concentration in the medium. Glucose caused catabolite repression of cell luminescence up to the late stationary phase, and ampicillin enhanced this effect. The feasibility of controlling the expression of cloned lux genes was shown.
WOS
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Popova L.I., Maksimova E.E., Kargatova T.V., Bril'kov A.V., Pechurkin N.S.
Заглавие : Introduction and long-term storage of recombinant luminescent Escherichia coli strain Z905 in laboratory water microecosystems
Место публикации : Izvestiia Akademii nauk. Seriia biologicheskaia / Rossiiskaia akademiia nauk. - 1998. - Is. 6. - С. 670-677. - ISSN 10263470 (ISSN)
Ключевые слова (''Своб.индексиров.''): article--bacterial gene--chemoluminescence--escherichia coli--gene expression--genetic recombination--genetics--laboratory--microbiology--chemiluminescent measurements--escherichia coli--gene expression--genes, bacterial--laboratories--recombination, genetic--water microbiology
Аннотация: We studied preservation of recombinant Escherichia coli strain Z905 (AprLux+) in liquid microecosystems (LME) after the introduction. E. coli cells were shown to remain viable and preserve the ability to express the cloned lux genes for a long time (more than a year) in LME. The majority of the clones have reduced efficiency of the expression due to either changed regulation of the lux operon or decreased number of copies of the plasmid. These mechanisms could be realized either independently or simultaneously depending on LME conditions. We have exposed the major factors affecting the metabolic activity of the E. coli strain Z905 (AprLux+) introduced into model ecosystems and the level of expression of the cloned genes.
Scopus
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Maksimova E.E., Popova L.Yu., Shpagina V.V., Belyavskaya V.A., Pechurkin N.S.
Заглавие : Effect of environmental factors on the expression of the catabolite-dependent lux-operon borne by a recombinant plasmid
Место публикации : Mikrobiologiya. - 1998. - Vol. 67, Is. 2. - С. 170-175. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): catabolite repression--environmental factors--escherichia coli--introduction into model ecosystems--lux-operon--recombinant plasmid--regulation of expression--recombinant dna--article--bacterial gene--chemoluminescence--culture medium--escherichia coli--gene expression regulation--genetics--microbiology--molecular cloning--operon--plasmid--chemiluminescent measurements--cloning, molecular--culture media--dna, recombinant--escherichia coli--gene expression regulation, bacterial--genes, bacterial--operon--plasmids--water microbiology
Аннотация: Expression of the lux-genes cloned on the recombinant plasmid pPHL7 (Ap rLux +) in Escherichia coli Z905 cells was studied in various environments, including model aquatic ecosystems. Expression of the lux-genes strongly depended on the nutritional status of the medium. In particular, the cultivation of cells in nutrient-rich medium favored the maintenance of the initial level of expression of the lux-operon, whereas nutrient limitation induced recombinant cell variants with an impaired control of the catabolite-dependent luxoperon. On the other hand, long-term laboratory cultivation of the recombinant strain in nutrient-deficient media or its long-term life in model aquatic ecosystems led to the accumulation of cells with a stringent control on the cloned lux-genes in the bacterial population. The presence of the selective factor (ampicillin) in the medium had no significant effect on the expression of the lux-operon.
Scopus
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Maksimova E.E., Popova L.Yu., Shpagina V.V., Belyavskaya V.A., Pechurkin N.S.
Заглавие : Effect of environmental factors on the expression of the catabolite-dependent lux-operon borne by a recombinant plasmid
Место публикации : Microbiology. - 1998. - Vol. 67, Is. 2. - С. 137-141. - ISSN 00262617 (ISSN)
Ключевые слова (''Своб.индексиров.''): catabolite repression--environmental factors--escherichia coli--introduction into model ecosystems--lux-operon--recombinant plasmid--regulation of expression
Аннотация: Expression of the lux-genes cloned in the recombinant plasmid pPHL7 (Ap1Lux+) in Escherichia coli Z905 cells was studied in various environments, including model aquatic ecosystems. Expression of the lux-genes strongly depended on the nutritional status of the medium. In particular, the cultivation of cells in nutrient-rich medium favored the maintenance of the initial level of expression of the lux-operon, whereas nutrient limitation induced recombinant cell variants with an impaired control of the catabolite-dependent luxoperon. On the other hand, long-term laboratory cultivation of the recombinant strain in nutrient-deficient media or its long-term life in model aquatic ecosystems led to the accumulation of cells with a stringent control on the cloned lux-genes in the bacterial population. The presence of the selective factor (ampicillin) in the medium had no significant effect on the expression of the lux-operon. В© 1998 MAHK Hayka/Interperiodica Publishing.
Scopus
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pechurkin N.S., Brilkov A.V., Ganusov V.V., Kargatova T.V., Maksimova E.E., Popova L.Yu.
Заглавие : Modelling of genetically engineered microorganisms introduction in closed artificial microcosms
Место публикации : Advances in Space Research. - 1999. - Vol. 24, Is. 3. - С. 335-341. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(99)00320-8
Ключевые слова (''Своб.индексиров.''): aquatic environment--artificial ecosystem--ecological modeling--genetically modified organism--alga--animal--article--bacterial count--bacterial gene--biological model--biomass--escherichia coli--feasibility study--genetic engineering--genetics--growth, development and aging--microbiology--microclimate--photobacterium--plasmid--protozoon--time--yeast--algae--animals--biomass--colony count, microbial--ecological systems, closed--escherichia coli--feasibility studies--genes, bacterial--genetic engineering--models, biological--photobacterium--plasmids--protozoa--time factors--water microbiology--yeasts
Аннотация: The possibility of introducing genetically engineered microorganisms (GEM) into simple biotic cycles of laboratory water microcosms was investigated. The survival of the recombinant strain Escherichia coli Z905 (Ap(r), Lux+) in microcosms depends on the type of model ecosystems. During the absence of algae blooming in the model ecosystem, the part of plasmid-containing cells E.coli decreased fast, and the structure of the plasmid was also modified. In conditions of algae blooming (Ankistrodesmus sp.) an almost total maintenance of plasmid-containing cells was observed in E.coli population. A mathematics model of GEM's behavior in water ecosystems with different level of complexity has been formulated. Mechanisms causing the difference in luminescent exhibition of different species are discussed, and attempts are made to forecast the GEM's behavior in water ecosystems.
Scopus
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : U - Popova LYu, Pechurkin N.S., Maksimova E.E., Kargatova T.V. U - Krylova TYu, Lobova T.I., Boyandin A.N.
Заглавие : Experimental microcosms as models of natural ecosystems for monitoring survival of genetically modified microorganism.
Место публикации : Life support & biosphere science : international journal of earth space. - 1999. - Vol. 6, Is. 3. - С. 193-197. - ISSN 10699422 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacterial dna--recombinant dna--adaptation--article--ecosystem--escherichia coli--genetics--microbiology--plasmid--risk assessment--adaptation, biological--dna, bacterial--dna, recombinant--ecosystem--escherichia coli--microbiology--plasmids--risk assessment--soil microbiology--water microbiology
Аннотация: An experimental approach for investigation of genetically modified microorganisms (GMMO) introduced into model ecosystems to evaluate potential risk of propagation of recombinant plasmids in surrounding medium has been developed. The object of modeling was Escherichia coli Z905 strain with a recombinant plasmid with bacterial luminescence genes, which was introduced into water microcosms of different structure. The approach involves comprehensive investigation of GMMO at four hierarchical levels: molecular (retaining the structure of the plasmid and expression of cloned genes); cellular (variation of metabolic activity); population (competitive power and metabolic interactions of GMMO with indigenous microflora, migration of recombinant and natural plasmids); ecosystem (effect of GMMO and cloned genes on ecosystem parameters). The experimental evidence and theoretical estimates are intended to form grounds to develop a basic version of an ecological certificate for different GMMO variants.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Lobova T.I., Krylova T.Yu., Kargatova T.V., Popova L.Yu., Pechurkin N.S.
Заглавие : Effect of salinity on the adaptive capacity of recombinant strains of Escherichia coli and Bacillus subtilis
Место публикации : Microbiology. - 2000. - Vol. 69, Is. 2. - С. 196-199. - ISSN 00262617 (ISSN)
Ключевые слова (''Своб.индексиров.''): genetically modified microorganisms--salinity--salt resistance
Аннотация: The effect of different concentrations of salts on natural and recombinant strains of Bacillus subtilis and Escherichia coli was studied. The recombinant strain of B. subtilis was found to be more osmotolerant than the wild-type strain of this bacterium, whereas the opposite situation was observed for the recombinant and wild-type strains of E. coli. Some salts exerted a bacteriostatic effect on E. coli and B. subtilis. The adaptive capacity of recombinant strains depended on the number of plasmid copies in the cells. The introduction of recombinant bacteria into model ecosystems resulted in the generation of their variants with increased osmotolerance. В© 2000 MAIK "Nauka/Interperiodica".
Scopus
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Krylova T.Yu., Popova L.Yu., Pechurkin N.S., Kashperova T.A., Belyavskaya V.A.
Заглавие : Population heterogeneity of plasmid-bearing and plasmid-free Bacillus subtilis strains under different environmental conditions
Место публикации : Microbiology. - 2000. - Vol. 69, Is. 2. - С. 220-224. - ISSN 00262617 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacillus subtilis--heterogeneity--introduction--microcosm--recombinant plasmid
Аннотация: The population heterogeneity of recombinant and plasmid-free Bacillus subtilis strains introduced into aquatic microcosms was studied. After introduction, the population of the plasmid-free strain B. subtilis 2335 in microcosms has long been represented by both vegetative cells and spores, whereas, already ten days after introduction, the population of the recombinant strain B. subtilis 2335/105 (KmI)Inf+) was represented only by spores. The number of plasmid copies in the spore isolates of the recombinant strain was the same as before introduction, but the plasmid abundance in the vegetative isolates of this strain decreased. The isolates of B. subtilis 2335/105 obtained from microcosms and the variants of this strain obtained by ten successive subcultures on M9 and 0.1?M9 media with and without kanamycin (Km) differed in the number of plasmid copies, Km resistance, and maximum biomass yield during batch cultivation. Irrespective of the presence of Km, more than 50% of the variants subcultured on M9 medium showed reduced plasmid abundance. At the same time, about 70% of the variants subcultured on 0.1 ? M9 medium with Km and 90% of the variants subcultured on the same medium without Km retained the initial number of plasmid copies. The variants subcultured on media with Km retained the initial biomass level. In more than 70% of the variants isolated from media without Km, the biomass yield increased. В© 2000 MAIK "Nauka/Interperiodica".
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Lobova T.I., Yu Krylova T., Kargatova T.V., Yu Popova L., Pechurkin N.S.
Заглавие : Effect of salinity on the adaptive capacity of recombinant strains of escherichia coli and bacillus subtilis
Место публикации : Mikrobiologiya. - 2000. - Vol. 69, Is. 2. - С. 243-247. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): genetically modified microorganisms--salinity--salt resistance--sodium chloride--adaptation--article--bacillus subtilis--electrolyte balance--escherichia coli--genetic recombination--genetics--physiology--adaptation, biological--bacillus subtilis--escherichia coli--recombination, genetic--sodium chloride--water-electrolyte balance
Аннотация: Effect of different concentrations of salts on natural and recombinant strains of Bacillus subtilis and Escherichia coli was studied. The recombinant strain of B. subtilis was found to be more osmotolerant than the wild-type strain of this bacterium, whereas the opposite situation was observed for the recombinant and wildtype strains of E. coli. Some salts exerted a bacteriostatic effect on E. coli and B. subtilis. The adaptive capacity of recombinant strains depended on the number of plasmid copies in the cells. The introduction of recombinant bacteria into model ecosystems resulted in the generation of their variants with increased osmotolerance.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Krylova T.Yu., Popova L.Yu., Pechurkin N.S., Kashperova T.A., Belyavskaya V.A.
Заглавие : Population heterogeneity of plasmid-bearing and plasmid-free bacillus subtilis strains under different environmental conditions
Место публикации : Mikrobiologiya. - 2000. - Vol. 69, Is. 2. - С. 270-275. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacillus subtilis--heterogeneity--introduction--microcosm--recombinant plasmid--article--bacillus subtilis--culture medium--genetic recombination--genetics--plasmid--bacillus subtilis--culture media--plasmids--recombination, genetic
Аннотация: The population heterogeneity of recombinant and plasmid-free Bacillus subtilis strains introduced into aquatic microcosms was studied. After introduction, the population of the plasmid-free strain B. subtilis 2335 in microcosms has long been represented by both vegetative cells and spores, whereas, already ten days after introduction, the population of the recombinant strain B. subtilis 2335/105 (Km rInf +) was represented only by spores. The number of plasmid copies in the spore isolates of the recombinant strain was the same as before introduction, but the plasmid abundance in the vegetative isolates of this strain decreased. The isolates of B. subtilis 2335/105 obtained from microcosms and the variants of this strain obtained by ten successive subcultures on M9 and 0.1 ? M9 media with and without kanamycin (Km) differed in the number of plasmid copies, Km resistance, and maximum biomass yield during batch cultivation. Irrespective of the presence of Km, more than 50% of the variants subcultured on M9 medium showed reduced plasmid abundance. At the same time, about 70% of the variants subcultured on 0.1 ? M9 medium with Km and 90% of the variants subcultured on the same medium without Km retained the initial number of plasmid copies. The variants subcultured on media with Km retained the initial biomass level. In more than 70% of the variants isolated from media without Km, the biomass yield increased.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ganusov V.V., Bril'kov A.V., Pechurkin N.S.
Заглавие : Mathematical modeling of population dynamics of unstable plasmid-containing bacteria during continuous cultivation in a chemostat
Место публикации : Biofizika. - 2000. - Vol. 45, Is. 5. - С. 908-914. - ISSN 00063029 (ISSN)
Ключевые слова (''Своб.индексиров.''): article--bioreactor--escherichia coli--fermentation--genetics--growth, development and aging--photobacterium--plasmid--theoretical model--bioreactors--escherichia coli--fermentation--models, theoretical--photobacterium--plasmids
Аннотация: A structural approach to studying the regularities of the population dynamics of unstable recombinant bacterial strains in a chemostat was elaborated. The approach is based on the mathematical modeling of cell distribution in a population with different numbers of plasmid copies. The effect of decreased selective preference of plasmidless variants of the recombinant strain in the chemostat, which is related to a decrease in the number of plasmid copies in cells upon long-term incubation was analyzed. It is shown that the time of half-elimination of plasmids from the bacterial population in the steady state in the chemostat T1/2 does not depend on the maximum number of plasmid copies in cells N but is determined only by the mean time of generation g and the probability of the loss of one plasmid copy tau. The dependence of the preference of bacterial plasmidless variants on the efficiency of expression of genes cloned into plasmids in chemostat was analyzed using the recombinant strain E. coli Z905, whose plasmids pPHL-7 contain cloned genes for the luminescence system of marine luminescing bacteria Photobacterium leiognathi.
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