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Каталог книг и продолжающихся изданий библиотеки Института биофизики СО РАН

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Труды сотрудников ИБФ СО РАН

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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Illarrionov B.A., Blinov V.M., Douchenko A.P., Protopopova M.V., Karginov V.A., Mertvetsov N.P., Gitelson J.I.
Заглавие : Isolation of bioluminescent functions from Photobacterium leiognathi: analysis of luxA, luxB, luxG and neighboring genes
Место публикации : Gene. - 1990. - Vol. 86, Is. 1. - С. 89-94. - ISSN 03781119 (ISSN)
Ключевые слова (''Своб.индексиров.''): bioluminescence--expression in e. coli--luciferase--molecular evolution--nucleotide sequence--protein alignment--recombinant dna--luciferase--amino acid sequence--article--bioluminescence--fungus--gene structure--genetic engineering--heredity--nonhuman--nucleotide sequence--priority journal--vibrionaceae--acyltransferases--amino acid sequence--bacterial proteins--base sequence--cloning, molecular--dna, bacterial--genes, structural, bacterial--luciferase--luminescence--molecular sequence data--operon--photobacterium--restriction mapping--escherichia coli--fungi--photobacterium leiognathi--vibrio harveyi--vibrionaceae
Аннотация: Genes encoding luminescence of Photobacterium leiognathi have been cloned in Escherichia coli. The luminescent clones were readily apparent. Among them, a clone containing a recombinant plasmid with a 13.5-kb insertion was identified. This DNA fragment contained all of the luminescence-encoding genes. The luciferase-encoding genes (lux) in this DNA fragment were localized. We have sequenced a part of the cloned lux region and identified the luxA, luxB and luxG genes encoding the ? and ? subunits of luciferase and a ? protein with an Mr of 26 180, respectively. The analysis of deduced amino acid sequences and comparison with known luciferase sequences from Vibrio harveyi, indicate the common origin of these proteins. В© 1990.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Popova L.Yu., Kargatova T.V., Ganusova E.E., Lobova T.I., Boyandin A.N., Mogilnaya O.A., Pechurkin N.S.
Заглавие : Population dynamics of transgenic strain Escherichia coli Z905/pPHL7 in freshwater and saline lake water microcosms with differing microbial community structures
Место публикации : Advances in Space Research. - 2005. - Vol. 35, Is. 9 SPEC. ISS. - С. 1573-1578. - ISSN 02731177 (ISSN) , DOI 10.1016/j.asr.2005.01.037
Ключевые слова (''Своб.индексиров.''): microcosm--plasmid--population dynamics--transgenic microorganism--bacteria--genes--microorganisms--saline water--microcosms--plasmid--population dynamics--transgenic microorganism--escherichia coli--brine--fresh water--inorganic salt--bacterial gene--conference paper--ecosystem--escherichia coli--gene expression regulation--genetics--growth, development and aging--microbiology--micrococcus--penicillin resistance--plasmid--population dynamics--transgenic organism--ampicillin resistance--ecosystem--environmental microbiology--escherichia coli--fresh water--gene expression regulation, bacterial--genes, bacterial--micrococcus--organisms, genetically modified--plasmids--population dynamics--salts--water microbiology
Аннотация: Populations of Escherichia coli Z905/pPHL7, a transgenic microorganism, were heterogenic in the expression of plasmid genes when adapting to the conditions of water microcosms of various mineralization levels and structure of microbial community. This TM has formed two subpopulations (ampicillin- resistant and ampicillin-sensitive) in every microcosm. Irrespective of mineralization level of a microcosm, when E. coli Z905/pPHL7 alone was introduced, the ampicillin-resistant subpopulation prevailed, while introduction of the TM together with indigenous bacteria led to the dominance of the ampicillin-sensitive subpopulation. A high level of lux gene expression maintained longer in the freshwater microcosms than in sterile saline lake water microcosms. A horizontal gene transfer has been revealed between the jointly introduced TM and Micrococcus sp. 9/pSH1 in microcosms with the Lake Shira sterile water. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Lobova T.I., Popova L.Yu., Pechurkin N.S.
Заглавие : Survival and alteration of the plasmid-containing microorganism Escherichia coli Z905/pPHL7 introduced into manmade closed aquatic microcosms
Место публикации : Advances in Space Research. - 2003. - Vol. 31, Is. 7. - С. 1763-1768. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(03)00118-2
Ключевые слова (''Своб.индексиров.''): cells--escherichia coli--salts--recombinant plamid--space research--ampicillin--chloride--inorganic salt--magnesium--mineral--potassium--sodium--sulfate--genetically modified organism--article--bacterial count--chemoluminescence--culture medium--drug effect--escherichia coli--genetics--growth, development and aging--microbiology--microclimate--penicillin resistance--plasmid--russian federation--transgenic organism--ampicillin--ampicillin resistance--chemiluminescent measurements--chlorides--colony count, microbial--culture media--ecological systems, closed--escherichia coli--magnesium--minerals--organisms, genetically modified--plasmids--potassium--russia--salts--sodium--sulfates--water microbiology
Аннотация: It has been demonstrated that the transgenic microorganism Escherichia coli Z905/pPHL7 (Ap'Lux+) can exist for a long time at an elevated concentration of mineral salts. The microorganism was introduced into microcosms with sterile brackish water (salinity variable from 21 to 22 g 1-1) taken from Lake Shira (Khakasia, Russia). The survivof the microorganism was estimated both by measuring the growth of the colonies on solid nutrient media and by the bioluminescence exhibited by the transgenic strain in samples from the microcosms and in the enrichment culture with the added selective factor - ampicillin (50 ?g/ml). In the enrichment culture, the bioluminescent signal was registered through the 160-day experiment. It has been shown that in the closed microcosms with brackish water the E. coli strain becomes heterogeneous in its ampicillin resistance. The populations of the transgenic strain were mainly represented by isolates able to persist in the medium containing 50 ?g/ml, but there were also the cells (about 10%) with the threshold of ampicillin resistance not more than 0.05 ?g/ml. Thus, it was shown that in the microcosms with brackish water and in the absence of the selective factor the transgenic strain survives and retains the recombinant plasmid. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kargatova T.V., Boyandin A.N., Popova L.Yu., Pechurkin N.S.
Заглавие : Experimental evaluation of the processes resulting from the introduction of the transgenic microorganism Escherichia coli Z905/pPHL7 (lux+) into aquatic microcosms
Место публикации : Advances in Space Research. - 2003. - Vol. 31, Is. 7. - С. 1769-1774. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(03)00119-4
Ключевые слова (''Своб.индексиров.''): escherichia coli--genes--sensitivity analysis--aquatic microcosms--space research--genetically modified organism--adaptation, physiological--ampicillin resistance--animals--chemiluminescent measurements--colony count, microbial--daphnia--ecological systems, closed--environmental monitoring--escherichia coli--fresh water--gene expression regulation, bacterial--organisms, genetically modified--plasmids--protozoa--time factors--water microbiology
Аннотация: The processes resulting from the introduction of the transgenic microorganism (TM) E. coli Z905/pPHL7 into aquatic microcosms have been modeled experimentally. It has been shown that the TM E. coli is able to adapt to a long co-existence with indigenous heterotrophic microflora in variously structured microcosms. In more complex microcosms the numerical dynamics of the introduced E. coli Z905/pPHL7 population is more stable. In the TM populations staying in the microcosms for a prolonged time, changes are recorded in the phenotypic expression of plasmid genes (ampicillin resistance and the luminescence level) and chromosome genes (morphological and physiological traits). However, in our study microcosms, the recombinant plasmid persisted in the TM cells for 6 years after the introduction, and as the population adapts to the conditions of the microcosms, the efficiency of the cloned gene expression in the cells is restored. In the microcosms with high microalgal counts (107 cells/ml), cells with a high threshold of sensitivity to ampicillin dominate in the population of the TM E. coli Z905/pPHL7. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Popova L.Yu., Lobova T.I., Krylova T.Yu., Kargatova T.V., Maksimova E.E., Boyandin A.N., Pechurkin N.S.
Заглавие : Population dynamics of transgenic microorganisms in the different microecosystem conditions
Место публикации : Advances in Space Research. - 2001. - Vol. 27, Is. 9. - С. 1571-1579. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(01)00248-4
Ключевые слова (''Своб.индексиров.''): ecosystems--escherichia coli--genes--interferons--luminescence--vaccines--microcosms--microorganisms--fresh water--microorganism--adaptation--article--bacillus subtilis--comparative study--ecosystem--escherichia coli--gene expression--genetics--growth, development and aging--hazardous waste--microbiology--micrococcus--osmotic pressure--plasmid--population dynamics--risk assessment--transgenic organism--adaptation, physiological--bacillus subtilis--containment of biohazards--ecosystem--escherichia coli--fresh water--gene expression--micrococcus--organisms, genetically modified--osmotic pressure--plasmids--population dynamics--risk assessment--water microbiology
Аннотация: The role of key environmental factors in adaptation of spore-forming and non-spore-forming transgenic microorganisms (TM) have been studied in model ecosystems. Model TM Escherichia coli Z905 (bearing plasmid genes of bacterial luminescence Ap1Lux+) has been found to have a higher adaptation potential than TM Bacillus subtilis 2335/105 (bearing genes of human ? 2-interferon Km1Inf+), planned for employment as a living vaccine under varying environmental conditions. Effects of abiotic factors on migration of natural and recombinant plasmids between microorganisms under model ecosystem conditions has been estimated. The transgenic microorganisms with low copy number survived better under introduction conditions in the microcosms studied. This trend has been shown to be independent of the microcosm type and its complexity. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Lobova T.I., Krylova T.Yu., Kargatova T.V., Popova L.Yu., Pechurkin N.S.
Заглавие : Effect of salinity on the adaptive capacity of recombinant strains of Escherichia coli and Bacillus subtilis
Место публикации : Microbiology. - 2000. - Vol. 69, Is. 2. - С. 196-199. - ISSN 00262617 (ISSN)
Ключевые слова (''Своб.индексиров.''): genetically modified microorganisms--salinity--salt resistance
Аннотация: The effect of different concentrations of salts on natural and recombinant strains of Bacillus subtilis and Escherichia coli was studied. The recombinant strain of B. subtilis was found to be more osmotolerant than the wild-type strain of this bacterium, whereas the opposite situation was observed for the recombinant and wild-type strains of E. coli. Some salts exerted a bacteriostatic effect on E. coli and B. subtilis. The adaptive capacity of recombinant strains depended on the number of plasmid copies in the cells. The introduction of recombinant bacteria into model ecosystems resulted in the generation of their variants with increased osmotolerance. В© 2000 MAIK "Nauka/Interperiodica".
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Krylova T.Yu., Popova L.Yu., Pechurkin N.S., Kashperova T.A., Belyavskaya V.A.
Заглавие : Population heterogeneity of plasmid-bearing and plasmid-free Bacillus subtilis strains under different environmental conditions
Место публикации : Microbiology. - 2000. - Vol. 69, Is. 2. - С. 220-224. - ISSN 00262617 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacillus subtilis--heterogeneity--introduction--microcosm--recombinant plasmid
Аннотация: The population heterogeneity of recombinant and plasmid-free Bacillus subtilis strains introduced into aquatic microcosms was studied. After introduction, the population of the plasmid-free strain B. subtilis 2335 in microcosms has long been represented by both vegetative cells and spores, whereas, already ten days after introduction, the population of the recombinant strain B. subtilis 2335/105 (KmI)Inf+) was represented only by spores. The number of plasmid copies in the spore isolates of the recombinant strain was the same as before introduction, but the plasmid abundance in the vegetative isolates of this strain decreased. The isolates of B. subtilis 2335/105 obtained from microcosms and the variants of this strain obtained by ten successive subcultures on M9 and 0.1?M9 media with and without kanamycin (Km) differed in the number of plasmid copies, Km resistance, and maximum biomass yield during batch cultivation. Irrespective of the presence of Km, more than 50% of the variants subcultured on M9 medium showed reduced plasmid abundance. At the same time, about 70% of the variants subcultured on 0.1 ? M9 medium with Km and 90% of the variants subcultured on the same medium without Km retained the initial number of plasmid copies. The variants subcultured on media with Km retained the initial biomass level. In more than 70% of the variants isolated from media without Km, the biomass yield increased. В© 2000 MAIK "Nauka/Interperiodica".
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Lobova T.I., Yu Krylova T., Kargatova T.V., Yu Popova L., Pechurkin N.S.
Заглавие : Effect of salinity on the adaptive capacity of recombinant strains of escherichia coli and bacillus subtilis
Место публикации : Mikrobiologiya. - 2000. - Vol. 69, Is. 2. - С. 243-247. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): genetically modified microorganisms--salinity--salt resistance--sodium chloride--adaptation--article--bacillus subtilis--electrolyte balance--escherichia coli--genetic recombination--genetics--physiology--adaptation, biological--bacillus subtilis--escherichia coli--recombination, genetic--sodium chloride--water-electrolyte balance
Аннотация: Effect of different concentrations of salts on natural and recombinant strains of Bacillus subtilis and Escherichia coli was studied. The recombinant strain of B. subtilis was found to be more osmotolerant than the wild-type strain of this bacterium, whereas the opposite situation was observed for the recombinant and wildtype strains of E. coli. Some salts exerted a bacteriostatic effect on E. coli and B. subtilis. The adaptive capacity of recombinant strains depended on the number of plasmid copies in the cells. The introduction of recombinant bacteria into model ecosystems resulted in the generation of their variants with increased osmotolerance.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Krylova T.Yu., Popova L.Yu., Pechurkin N.S., Kashperova T.A., Belyavskaya V.A.
Заглавие : Population heterogeneity of plasmid-bearing and plasmid-free bacillus subtilis strains under different environmental conditions
Место публикации : Mikrobiologiya. - 2000. - Vol. 69, Is. 2. - С. 270-275. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacillus subtilis--heterogeneity--introduction--microcosm--recombinant plasmid--article--bacillus subtilis--culture medium--genetic recombination--genetics--plasmid--bacillus subtilis--culture media--plasmids--recombination, genetic
Аннотация: The population heterogeneity of recombinant and plasmid-free Bacillus subtilis strains introduced into aquatic microcosms was studied. After introduction, the population of the plasmid-free strain B. subtilis 2335 in microcosms has long been represented by both vegetative cells and spores, whereas, already ten days after introduction, the population of the recombinant strain B. subtilis 2335/105 (Km rInf +) was represented only by spores. The number of plasmid copies in the spore isolates of the recombinant strain was the same as before introduction, but the plasmid abundance in the vegetative isolates of this strain decreased. The isolates of B. subtilis 2335/105 obtained from microcosms and the variants of this strain obtained by ten successive subcultures on M9 and 0.1 ? M9 media with and without kanamycin (Km) differed in the number of plasmid copies, Km resistance, and maximum biomass yield during batch cultivation. Irrespective of the presence of Km, more than 50% of the variants subcultured on M9 medium showed reduced plasmid abundance. At the same time, about 70% of the variants subcultured on 0.1 ? M9 medium with Km and 90% of the variants subcultured on the same medium without Km retained the initial number of plasmid copies. The variants subcultured on media with Km retained the initial biomass level. In more than 70% of the variants isolated from media without Km, the biomass yield increased.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ganusov V.V., Bril'kov A.V., Pechurkin N.S.
Заглавие : Mathematical modeling of population dynamics of unstable plasmid-containing bacteria during continuous cultivation in a chemostat
Место публикации : Biofizika. - 2000. - Vol. 45, Is. 5. - С. 908-914. - ISSN 00063029 (ISSN)
Ключевые слова (''Своб.индексиров.''): article--bioreactor--escherichia coli--fermentation--genetics--growth, development and aging--photobacterium--plasmid--theoretical model--bioreactors--escherichia coli--fermentation--models, theoretical--photobacterium--plasmids
Аннотация: A structural approach to studying the regularities of the population dynamics of unstable recombinant bacterial strains in a chemostat was elaborated. The approach is based on the mathematical modeling of cell distribution in a population with different numbers of plasmid copies. The effect of decreased selective preference of plasmidless variants of the recombinant strain in the chemostat, which is related to a decrease in the number of plasmid copies in cells upon long-term incubation was analyzed. It is shown that the time of half-elimination of plasmids from the bacterial population in the steady state in the chemostat T1/2 does not depend on the maximum number of plasmid copies in cells N but is determined only by the mean time of generation g and the probability of the loss of one plasmid copy tau. The dependence of the preference of bacterial plasmidless variants on the efficiency of expression of genes cloned into plasmids in chemostat was analyzed using the recombinant strain E. coli Z905, whose plasmids pPHL-7 contain cloned genes for the luminescence system of marine luminescing bacteria Photobacterium leiognathi.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kargatova T.V., Maksimova E.E., Popova L.Yu., Bril'Kov A.V., Pechurkin N.S.
Заглавие : Phenotypic variability of the population of a recombinant luminescent strain of escherichia coli in aqueous microcosms
Место публикации : Mikrobiologiya. - 1997. - Vol. 66, Is. 1. - С. 101-106. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): article--escherichia coli--genetic recombination--genetics--luminescence--microbiology--penicillin resistance--phenotype--plasmid--ampicillin resistance--escherichia coli--luminescent measurements--phenotype--plasmids--recombination, genetic--water microbiology
Аннотация: The behavior of Escherichia coli Z905, carrying a recombinant plasmid pPHL7 with genes determining ampicillin resistance and bacterial luminescence, and the efficiency of expression of cloned genes were studied after introduction of the strain into model aqueous ecosystems with different trophic chain lengths. The E. coli Z905 variants isolated from ecosystems after different periods of time were found to vary in their resistance to ampicillin (from 50 to 0.05 ?g/ml) and in the intensity of bioluminescence. An increase in the concentration of the selective factor (ampicillin) or in the extent of the aqueous microcosm blooming restored the expression of the recombinant plasmid genes in some clones.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kargatova T.V., Maksimova E.E., Popova L.Yu., Bril'kov A.V., Pechurkin N.S.
Заглавие : Phenotypic variability of the population of a recombinant luminescent strain of escherichia coli in aqueous microcosms
Место публикации : Microbiology. - 1997. - Vol. 66, Is. 1. - С. 85-90. - ISSN 00262617 (ISSN)
Аннотация: The behavior of Escherichia coli Z905, carrying a recombinant plasmid pPHL7 with genes determining ampicillin resistance and bacterial luminescence, and the efficiency of expression of cloned genes were studied after introduction of the strain into model aqueous ecosystems with different trophic chain lengths. The E. coli Z905 variants isolated from ecosystems after different periods of time were found to vary in their resistance to ampicillin (from 50 to 0.05 ?g/ml) and in the intensity of bioluminescence. An increase in the concentration of the selective factor (ampicillin) or in the extent of the aqueous microcosm blooming restored the expression of the recombinant plasmid genes in some clones. В© 1997 MAHK Hayka/Interperiodica Publishing.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Maksimova E.E., Popova L.Yu., Shpagina V.V., Belyavskaya V.A., Pechurkin N.S.
Заглавие : Effect of environmental factors on the expression of the catabolite-dependent lux-operon borne by a recombinant plasmid
Место публикации : Microbiology. - 1998. - Vol. 67, Is. 2. - С. 137-141. - ISSN 00262617 (ISSN)
Ключевые слова (''Своб.индексиров.''): catabolite repression--environmental factors--escherichia coli--introduction into model ecosystems--lux-operon--recombinant plasmid--regulation of expression
Аннотация: Expression of the lux-genes cloned in the recombinant plasmid pPHL7 (Ap1Lux+) in Escherichia coli Z905 cells was studied in various environments, including model aquatic ecosystems. Expression of the lux-genes strongly depended on the nutritional status of the medium. In particular, the cultivation of cells in nutrient-rich medium favored the maintenance of the initial level of expression of the lux-operon, whereas nutrient limitation induced recombinant cell variants with an impaired control of the catabolite-dependent luxoperon. On the other hand, long-term laboratory cultivation of the recombinant strain in nutrient-deficient media or its long-term life in model aquatic ecosystems led to the accumulation of cells with a stringent control on the cloned lux-genes in the bacterial population. The presence of the selective factor (ampicillin) in the medium had no significant effect on the expression of the lux-operon. В© 1998 MAHK Hayka/Interperiodica Publishing.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Maksimova E.E., Popova L.Yu., Shpagina V.V., Belyavskaya V.A., Pechurkin N.S.
Заглавие : Effect of environmental factors on the expression of the catabolite-dependent lux-operon borne by a recombinant plasmid
Место публикации : Mikrobiologiya. - 1998. - Vol. 67, Is. 2. - С. 170-175. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): catabolite repression--environmental factors--escherichia coli--introduction into model ecosystems--lux-operon--recombinant plasmid--regulation of expression--recombinant dna--article--bacterial gene--chemoluminescence--culture medium--escherichia coli--gene expression regulation--genetics--microbiology--molecular cloning--operon--plasmid--chemiluminescent measurements--cloning, molecular--culture media--dna, recombinant--escherichia coli--gene expression regulation, bacterial--genes, bacterial--operon--plasmids--water microbiology
Аннотация: Expression of the lux-genes cloned on the recombinant plasmid pPHL7 (Ap rLux +) in Escherichia coli Z905 cells was studied in various environments, including model aquatic ecosystems. Expression of the lux-genes strongly depended on the nutritional status of the medium. In particular, the cultivation of cells in nutrient-rich medium favored the maintenance of the initial level of expression of the lux-operon, whereas nutrient limitation induced recombinant cell variants with an impaired control of the catabolite-dependent luxoperon. On the other hand, long-term laboratory cultivation of the recombinant strain in nutrient-deficient media or its long-term life in model aquatic ecosystems led to the accumulation of cells with a stringent control on the cloned lux-genes in the bacterial population. The presence of the selective factor (ampicillin) in the medium had no significant effect on the expression of the lux-operon.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Popova L.I., Maksimova E.E., Kargatova T.V., Bril'kov A.V., Pechurkin N.S.
Заглавие : Introduction and long-term storage of recombinant luminescent Escherichia coli strain Z905 in laboratory water microecosystems
Место публикации : Izvestiia Akademii nauk. Seriia biologicheskaia / Rossiiskaia akademiia nauk. - 1998. - Is. 6. - С. 670-677. - ISSN 10263470 (ISSN)
Ключевые слова (''Своб.индексиров.''): article--bacterial gene--chemoluminescence--escherichia coli--gene expression--genetic recombination--genetics--laboratory--microbiology--chemiluminescent measurements--escherichia coli--gene expression--genes, bacterial--laboratories--recombination, genetic--water microbiology
Аннотация: We studied preservation of recombinant Escherichia coli strain Z905 (AprLux+) in liquid microecosystems (LME) after the introduction. E. coli cells were shown to remain viable and preserve the ability to express the cloned lux genes for a long time (more than a year) in LME. The majority of the clones have reduced efficiency of the expression due to either changed regulation of the lux operon or decreased number of copies of the plasmid. These mechanisms could be realized either independently or simultaneously depending on LME conditions. We have exposed the major factors affecting the metabolic activity of the E. coli strain Z905 (AprLux+) introduced into model ecosystems and the level of expression of the cloned genes.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pechurkin N.S., Brilkov A.V., Ganusov V.V., Kargatova T.V., Maksimova E.E., Popova L.Yu.
Заглавие : Modelling of genetically engineered microorganisms introduction in closed artificial microcosms
Место публикации : Advances in Space Research. - 1999. - Vol. 24, Is. 3. - С. 335-341. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(99)00320-8
Ключевые слова (''Своб.индексиров.''): aquatic environment--artificial ecosystem--ecological modeling--genetically modified organism--alga--animal--article--bacterial count--bacterial gene--biological model--biomass--escherichia coli--feasibility study--genetic engineering--genetics--growth, development and aging--microbiology--microclimate--photobacterium--plasmid--protozoon--time--yeast--algae--animals--biomass--colony count, microbial--ecological systems, closed--escherichia coli--feasibility studies--genes, bacterial--genetic engineering--models, biological--photobacterium--plasmids--protozoa--time factors--water microbiology--yeasts
Аннотация: The possibility of introducing genetically engineered microorganisms (GEM) into simple biotic cycles of laboratory water microcosms was investigated. The survival of the recombinant strain Escherichia coli Z905 (Ap(r), Lux+) in microcosms depends on the type of model ecosystems. During the absence of algae blooming in the model ecosystem, the part of plasmid-containing cells E.coli decreased fast, and the structure of the plasmid was also modified. In conditions of algae blooming (Ankistrodesmus sp.) an almost total maintenance of plasmid-containing cells was observed in E.coli population. A mathematics model of GEM's behavior in water ecosystems with different level of complexity has been formulated. Mechanisms causing the difference in luminescent exhibition of different species are discussed, and attempts are made to forecast the GEM's behavior in water ecosystems.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : U - Popova LYu, Pechurkin N.S., Maksimova E.E., Kargatova T.V. U - Krylova TYu, Lobova T.I., Boyandin A.N.
Заглавие : Experimental microcosms as models of natural ecosystems for monitoring survival of genetically modified microorganism.
Место публикации : Life support & biosphere science : international journal of earth space. - 1999. - Vol. 6, Is. 3. - С. 193-197. - ISSN 10699422 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacterial dna--recombinant dna--adaptation--article--ecosystem--escherichia coli--genetics--microbiology--plasmid--risk assessment--adaptation, biological--dna, bacterial--dna, recombinant--ecosystem--escherichia coli--microbiology--plasmids--risk assessment--soil microbiology--water microbiology
Аннотация: An experimental approach for investigation of genetically modified microorganisms (GMMO) introduced into model ecosystems to evaluate potential risk of propagation of recombinant plasmids in surrounding medium has been developed. The object of modeling was Escherichia coli Z905 strain with a recombinant plasmid with bacterial luminescence genes, which was introduced into water microcosms of different structure. The approach involves comprehensive investigation of GMMO at four hierarchical levels: molecular (retaining the structure of the plasmid and expression of cloned genes); cellular (variation of metabolic activity); population (competitive power and metabolic interactions of GMMO with indigenous microflora, migration of recombinant and natural plasmids); ecosystem (effect of GMMO and cloned genes on ecosystem parameters). The experimental evidence and theoretical estimates are intended to form grounds to develop a basic version of an ecological certificate for different GMMO variants.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Попова Л.Ю., Луцкая Н.И., Богучарова А.А., Брильков А.В., Печуркин Н.С.
Заглавие : Динамика структуры популяции рекомбинантного штамма Escherichia coli при непрерывном культивировании : научное издание
Место публикации : Микробиология. - 1992. - Т. 61, N 4. - С. 598-603. - ISSN 0026-3656
ГРНТИ : 62.37.99
Предметные рубрики: КУЛЬТИВИРОВАНИЕНЕПРЕРЫВНОЕ
ESCHERICHIACOLI
РЕКОМБИНАНТНЫЙШТАММ
ПЛАЗМИДА
УСЛОВИЯСОХРАНЕНИЯВПОПУЛЯЦИИКЛЕТОК
CONTINUOUSCULTURE
PLASMID
Аннотация: Исследовали структуры популяции штамма Escherichia coli Z905, несущего рекомбинантную плазмиду с фенотипом Ap{r} ux{+}, при непрерывном культивировании в хемостате. Показано, что устойчивое поддержание определенного соотношения числа бесплазмидных и плазмидных клеток в популяции зависит прежде всего от наличия в среде селективного фактора (ампициллина), а также от типа источника углерода и энергии, лимитирующего рост. СНГ, Ин-т биофизики СО РАН, Красноярск. Библ. 10
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gusev A.A., Kargatova T.V., Medvedeva S.E., Popova L.Yu.
Заглавие : Quantitative criteria for estimating the effectiveness of bioluminescence expression in natural and transgenic luminescent bacteria
Место публикации : Biophysics. - 2008. - Vol. 53, Is. 5. - С. 417-420. - ISSN 00063509 (ISSN) , DOI 10.1134/S0006350908050175
Ключевые слова (''Своб.индексиров.''): effectiveness of lux-operon expression--natural and transgenic bacteria--bacteria (microorganisms)--escherichia coli--photobacterium leiognathi
Аннотация: Computational coefficients for estimating the effectiveness of bioluminescence expression in natural luminescent bacteria Photobacterium leiognathi 54 and transgenic strain E. coli Z905/pPHL7 bearing lux-operon in a multicopy plasmid are suggested and their use at molecular, cell, and population levels was considered. It was shown that at the population level, all transgenic variants have an advantage over natural variants of P. leiognathi 54 irrespective of the type of lux-operon regulation. At the cell level, the effectiveness of bioluminescence expression in the bright and dim variants of the transgenic strain increased by several orders. At the level of one lux-operon, the effectiveness of expression in the bright variant of the transgenic strain is substantially higher than in the natural bright variant; in dim variants, the efficiency values are similar; the effectiveness of bioluminescence expression in the dark variant of E. coli Z905-2/pPHL7 is by two orders of magnitude lower than in the dark variant of P. leiognathi 54. В© 2008 Pleiades Publishing, Ltd.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Popova L.Y.
Заглавие : Salt-dependent inhibition of light emitting of the luminescent microorganism Escherichia coli Z9051
Место публикации : Biofizika: MEZHDUNARODNAYA KNIGA, 2001. - Vol. 46, Is. 2. - С. 251-255. - 5. - ISSN 0006-3029
Примечания : Cited References: 12
Ключевые слова (''Своб.индексиров.''): bacterial luminescence--recombinant plasmid--salt concentration
Аннотация: The influence of some mineral salts on the recombinant strain Escherichia coli Z9051 was investigated. It was shown that the composition (NaCl, Na2SO4, MgCl2 and MgSO4) and concentration (5 and 10%) of the salts substantially affect the expression of genes for the luminescence system of fight-emitting bacteria cloned in the plasmid under the control of the lac-promoter. In some cases, the luminescence level of the microorganism in the presence of salts was similar to the luminescence level under catabolite repression by glucose, the more strong influence of the salts exceeding the effect of catabolite repression. The possibility of adaptation of the genetically modified microorganism to the salinity factor is discussed.
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