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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G.
Заглавие : A comparative investigation of biodegradable polyhydroxyalkanoate films as matrices for in vitro cell cultures
Место публикации : J. Mater. Sci.-Mater. Med.: KLUWER ACADEMIC PUBL, 2004. - Vol. 15, Is. 8. - С. 915-923. - 9. - ISSN 0957-4530, DOI 10.1023/B:JMSM.0000036280.98763.c1
Примечания : Cited References: 34
Предметные рубрики: DEGRADATION
POLY(3-HYDROXYBUTYRATE)
POLYESTERS
POLYMERS
Аннотация: The paper describes the production and investigation of flexible films made of high-purity polyhydroxyalkanoates (PHAs) - polyhydroxybutyrate [poly-(3HB)] and poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate [poly(3Hl3-co-3HV)], containing 4-30 mol % hydroxyvalerate. Poly(3HB-co-3HV) films have a more porous structure than poly-(3HB) films, which are more compact, but their surface properties, such as wettability and surface and interface energies, are the same. Sterilisation of the PHA films by conventional methods (heat treatment and gamma-irradiation) did not impair their strength. Cells cultured on PHA films exhibited high levels of cell adhesion. Cell morphology, protein synthesis and DNA synthesis were estimated by extent of H-3-thymidine incorporation into the animal cell cultures of various origins (fibroblasts, endothelium cells, and isolated hepatocytes) in direct contact with PHAs. The investigation showed that this material can be used to make matrices for in vitro proliferous cells. The investigated properties of poly-(3HB) and poly(3HB-co-3HV) films proved to be fundamentally similar. (C) 2004 Kluwer Academic Publishers.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Kalacheva G.S., Rodicheva E.K., Volova T.G.
Заглавие : Luminous bacteria as potential producers of resorbed polyhydroxyalkanoate polyesters
Место публикации : Dokl. Biochem. Biophys.: SPRINGER, 2007. - Vol. 416, Is. 01.06.2013. - С. 248-251. - 4. - ISSN 1607-6729, DOI 10.1134/S1607672907050067
Примечания : Cited References: 15
Предметные рубрики: VIBRIO-HARVEYI
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prudnikova S.V., Boyandin A.N., Kalacheva G.S., Sinskey A.J.
Заглавие : Degradable Polyhydroxyalkanoates as Herbicide Carriers
Коллективы : Government of the Russian Federation [11.G34.31.0013]
Место публикации : J. Polym. Environ.: SPRINGER/PLENUM PUBLISHERS, 2013. - Vol. 21, Is. 3. - С. 675-682. - 8. - ISSN 1566-2543, DOI 10.1007/s10924-012-0561-z
Примечания : Cited References: 31. - We thank Dr. Christopher Brigham for critical review of the manuscript prior to submission. The study was supported by the project initiated by the Government of the Russian Federation for governmental support of scientific research conducted under the guidance of leading scientists at Russian institutions of higher learning (Agreement No. 11.G34.31.0013).
Предметные рубрики: CONTROLLED-RELEASE
SOIL
BIODEGRADATION
POLYESTERS
PESTICIDE
POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)
MICROSPHERES
BACTERIA
Ключевые слова (''Своб.индексиров.''): herbicide--haloxyfop-p-methyl--zellek super--sustained-release formulations--polyhydroxyalkanoates--plant growth suppression
Аннотация: The biodegradable polymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) has been used to design experimental sustained-release formulations of the herbicide Zellek Super in the form of films and microgranules. The kinetics of polymer degradation and the dynamics of herbicide release show that the rate and extent of herbicide release from the polymer matrix into the soil depends on the geometry of the carrier and the proportion of the pesticide loaded into it (polymer/pesticide mass ratio). Experiments with the creeping bentgrass (Agrostis stolonifera L.) show that the formulations of the herbicide Zellek Super constructed as microgranules and films can be successfully used to suppress the growth of grasses. This study is the first to demonstrate that biodegradable polyhydroxyalkanoates can be used effectively to construct environmentally friendly sustained-release PHA-herbicide systems that can be placed into the soil together with seeds.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Murueva A.V., Shershneva A.M., Shishatskaya E.I., Volova T.G.
Заглавие : The Use of Polymeric Microcarriers Loaded with Anti-Inflammatory Substances in the Therapy of Experimental Skin Wounds
Колич.характеристики :6 с
Место публикации : Bulletin of Experimental Biology and Medicine. - 2014. - ISSN 00074888 (ISSN) , DOI 10.1007/s10517-014-2624-8
Аннотация: We studied the effects of anti-inflammatory substances incorporated in polymeric microparticles made of degradable natural polyhydroxyalkanoate polyesters on experimental skin wounds caused by chemical burns in laboratory animals. Treatment with encapsulated forms of anti-inflammatory substances (applied in gel) accelerated wound healing in comparison with routine therapy (estimated by area of burn wound, wound healing activity, number of acanthotic cells, and number of hair and sebaceous follicles). The results showed the perspectives of usage of developed form of substances (degradable polymeric microparticles) for treatment of skin defects.
Scopus
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Eke G..., Kuzmina A.M., Goreva A.V., Shishatskaya E.I., Hasirci N..., Hasirci V...
Заглавие : In vitro and transdermal penetration of PHBV micro/nanoparticles
Колич.характеристики :11 с
Коллективы : Government of the Russian Federation [220, 11.G34.31.0013, MD-3112.2012.4]; EC FP7 SKINTREAT project; State Planning Organization (Turkey)
Место публикации : J. Mater. Sci.-Mater. Med.: SPRINGER, 2014. - Vol. 25, Is. 6. - С. 1471-1481. - ISSN 0957-4530, DOI 10.1007/s10856-014-5169-5. - ISSN 1573-4838
Примечания : Cited References: 38. - The study was supported by the Government of the Russian Federation (Decree No. 220 of 09.04.2010) (Agreement No. 11.G34.31.0013) and (Grant No MD-3112.2012.4). We gratefully acknowledge the EC FP7 SKINTREAT project and the State Planning Organization (Turkey) for the grant to establish BIOMATEN. Mr. A. Buyuksungur is acknowledged for his contributions with CLSM.
Предметные рубрики: DRUG-DELIVERY
PLGA NANOPARTICLES
CELLULAR UPTAKE
MICROPARTICLES
POLYHYDROXYALKANOATES
CYTOTOXICITY
SIZE
POLYESTERS
MECHANISM
CELLS
Аннотация: The purpose of this study was to develop micro and nano sized drug carriers from poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), and study the cell and skin penetration of these particles. PHBV micro/nanospheres were prepared by o/w emulsion method and were stained with a fluorescent dye, Nile Red. The particles were fractionated by centrifugation to produce different sized populations. Topography was studied by SEM and average particle size and its distribution were determined with particle sizer. Cell viability assay (MTT) was carried out using L929 fibroblastic cell line, and particle penetration into the cells were studied. Transdermal permeation of PHBV micro/nanospheres and tissue reaction were studied using a BALB/c mouse model. Skin response was evaluated histologically and amount of PHBV in skin was determined by gas chromatography-mass spectrometry. The average diameters of the PHBV micro/nanosphere batches were found to be 1.9 mu m, 426 and 166 nm. Polydispersity indices showed that the size distribution of micro sized particles was broader than the smaller ones. In vitro studies showed that the cells had a normal growth trend. MTT showed no signs of particle toxicity. The 426 and 166 nm sized PHBV spheres were seen to penetrate the cell membrane. The histological sections revealed no adverse effects. In view of this data nano and micro sized PHBV particles appeared to have potential to serve as topical and transdermal drug delivery carriers for use on aged or damaged skin or in cases of skin diseases such as psoriasis, and may even be used in gene transfer to cells.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Voinova O..., Gladyshev M.I., Volova T.G.
Заглавие : Comparative study of PHA degradation in natural reservoirs having various types of ecosystems
Место публикации : Macromol. Symp.: WILEY-V C H VERLAG GMBH, 2008. - Vol. 269: 4th European Symposium on Biopolymers (OCT 02-04, 2007, Kusadasi, TURKEY). - С. 34-37. - 4. - ISSN 1022-1360, DOI 10.1002/masy.zoo850906
Примечания : Cited References: 15
Предметные рубрики: POLYHYDROXYALKANOATES
BIODEGRADATION
DISAPPEARANCE
POLYESTERS
PHENOL
Ключевые слова (''Своб.индексиров.''): biodegradable polyesters--ecosystem--natural environment--pha--water reservoir
Аннотация: The kinetics of polyhydroxyalkanoate (PHA) degradation in natural environment in two water reservoirs having various ecological characteristics was studied. It was shown that biodegradation of polymer essentially depends on the environment temperature and inorganic composition of water. The processes of polymer degradation under aerobic and anaerobic conditions were compared. The polymer degradation was slower under anaerobic conditions.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I.
Заглавие : Rubomycin microincapsulation with biodegradable polymer matrix
Место публикации : Antibiotiki i Khimioterapiya. - 2005. - Vol. 50, Is. 8-9. - С. 4-9. - ISSN 02352990 (ISSN)
Ключевые слова (''Своб.индексиров.''): daunorubicin (rubomycin)--microspheres--polyhydroxybutirate--antineoplastic antibiotic--daunorubicin--hydroxybutyric acid--microsphere--poly(3 hydroxybutyric acid)--poly-beta-hydroxybutyrate--polyester--polymer--article--chemistry--delayed release formulation--electronics--methodology--microcapsule--temperature--antibiotics, antineoplastic--capsules--daunorubicin--delayed-action preparations--hydroxybutyrates--microspheres--miniaturization--polyesters--polymers--temperature
Аннотация: A procedure for preparation of microspheres from biodegradable linear polyether of microbiological origin (polyhydroxybutirate, PHB) with using the technology of solvent evaporation was developed considering a specific example of two- and three-component emulsions. The procedure provided permanent preparation of the microspheres of high quality. The influence of the procedure (emulsion type, dispersion process and medium temperature) on the yield of the microspheres, their structure and size was shown. The temperature had a significant impact on incorporation of the antitumor anthracycline antibiotic daunorubicin (rubomycin) to the polymer matrix. The microspheres with various levels of the drug load (29 and 90% of the initial content in the emulsion) were prepared and the kinetics of the in vitro rubomycin release was studied. The dynamics of the highly toxic rubomycin release from the microspheres was on the whole even with the curve profile reaching the plateau in 20-22 hours of the observation period. The rate of the rubomycin release to the medium depended on the value of the antibiotic incorporation and was maximum within the first two hours (3.3 and 13.0 mcg/mlВ·h) that corresponded to the release of 0.97 and 3.89 of the incorporated antibiotic. The average rate of rubomycin release during 300 hours was 0.81В·10-4 and 2.3В·10-4 mcg/mlВ·h. The release constituted respectively 3.9 and 13.11% of the antibiotic incorporated to the microspheres.
Scopus
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I.
Заглавие : Microencapculation of daunorubicin with biodegradable polymer matrix
Место публикации : Antibiotiki i Khimioterapiya. - 2007. - Vol. 52, Is. 9-10. - С. 3-8. - ISSN 02352990 (ISSN)
Ключевые слова (''Своб.индексиров.''): daunorubicin--microencapsulation--polymer matrix--antineoplastic antibiotic--daunorubicin--polyester--animal--article--bagg albino mouse--chemistry--kinetics--microcapsule--mouse--particle size--animals--antibiotics, antineoplastic--capsules--daunorubicin--kinetics--mice--mice, inbred balb c--particle size--polyesters--animalia
Аннотация: Procedure for microencapsulation providing stable formation of high quality microspheres was designed. Conditions for deposition of the anthracycline antibiotic daunorubicin to polymer matrix were developed and microsheres loaded with various quantities of the drug were prepared. The kinetics of the in vitro and in vivo release of daunorubicin from the microsheres was studied. The rate of the rubomycin release to the medium in vitro (balanced phosphate buffer at 37.8В°C) in a 300-hour experiment directly depended on the quantity of the incorporated drug and averaged 0.81 В· 10 -4 to 2.3 В· 10 -4 mcg/mlВ·h. The experiment on laboratory animals with intraperitoneal administration of the rubomycin microsperes showed that the drug remained in the blood and abdominal liquid for a long time (up to 10 days). Possible control of the quantity of the rubomycin encapsulation to the polymer matrix, no sharp efflux of the drug at the early stages of the observation and low rate of the drug release to the medium allowed to conclude that the use of the biodegradable polymer microsperes as carriers of the high toxic antibiotic providing its prolonged action was prospective.
Scopus
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Prudnikova S.V., Karpov V.A., Ivonin V.N., Do N.L., Nguyen T.H., Le TMH, Filichev N.L., Levin A.L., Filipenko M.L., Volova T.G., Gitelson I.I.
Заглавие : Microbial degradation of polyhydroxyalkanoates in tropical soils
Место публикации : Int. Biodeterior. Biodegrad.: ELSEVIER SCI LTD, 2013. - Vol. 83. - С. 77-84. - 8. - ISSN 0964-8305, DOI 10.1016/j.ibiod.2013.04.014
Примечания : Cited References: 74. - This study was financially supported by Project "Biotechnologies of novel biomaterials" (Agreement No. 11.G34.31.0013) in accordance with Resolution No. 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning".
Предметные рубрики: POLY-BETA-HYDROXYBUTYRATE
CHAIN-LENGTH POLYHYDROXYALKANOATES
POLY(3-HYDROXYBUTYRATE) DEPOLYMERASE
COASTAL WATERS
PHA FILMS
BIODEGRADATION
BACTERIA
IDENTIFICATION
POLYESTERS
POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)
Ключевые слова (''Своб.индексиров.''): polyhydroxyalkanoates (phas)--biopolymers--biopolymer properties--soil biodegradation--pha degrading microorganisms
Аннотация: The integrated study addressing biodegradation of microbial linear polyesters of hydroxyalkanoic acids (polyhydroxyalkanoates, PHAs) in tropical conditions by microbial communities of Vietnamese soils was performed in locations close to Hanoi and Nha Trang, which differed in their weather conditions and microbial communities. It shows that PHA degradation in tropical soils is influenced by polymer chemical composition, specimen shape, and microbial characteristics. The homopolymer of 3-hydroxybutyric acid is degraded at higher rates than the copolymer of 3-hydroxybutyric and 3-hydroxyvaleric acids. The average rates of mass loss were 0.04-0.33% per day for films and 0.02-0.18% for compact pellets. PHA degradation was accompanied by a decrease in the polymer molecular mass and, usually, an increase in the degree of crystallinity, suggesting preferential degradation of the amorphous phase. Under the study conditions, representatives of the bacterial genera Burkholderia, Bacillus, Cupriavidus, Mycobacterium, and Nocardiopsis and such micromycetes as Acremonium, Gongronella, Paecilomyces, and Penicillium, Trichoderma have been identified as major PHA degraders. (C) 2013 Elsevier Ltd. All rights reserved.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A..., Kalacheva G.S., Medvedeva S..., Rodicheva E..., Volova T.G.
Заглавие : Luminous bacteria as producers of polyhydroxyalkanoates
Место публикации : Macromol. Symp.: WILEY-V C H VERLAG GMBH, 2008. - Vol. 269: 4th European Symposium on Biopolymers (OCT 02-04, 2007, Kusadasi, TURKEY). - С. 17-22. - 6. - ISSN 1022-1360, DOI 10.1002/masy.200850904
Примечания : Cited References: 16
Предметные рубрики: VIBRIO-HARVEYI
BIOLUMINESCENCE
Ключевые слова (''Своб.индексиров.''): luminous bacteria--polyhydroxyalkanoates--polyhydroxybutyrate
Аннотация: The study addresses the ability of luminous bacteria of different taxa (Photobacterium leiognathi, Photobacterium phosphoreum, Vibrio harveyi, Vibrio fischeri) to synthesize polyesters of hydrocarbon acids (polyhydroxyalkanoates, PHAs) as storage macromolecules. The screened strains widely varied in their PHA productivity. Conditions for attaining high polymer yields (including two- and three-component polymers) in batch culture have been determined. The attained polymer yields reached 40-70% of dry cell biomass. The results suggest a conclusion that luminous microorganisms can be considered as producers of multi-component PHAs.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Goreva A.V., Shishatskaya E.I., Volova T.G., Sinskey A.J.
Заглавие : Characterization of polymeric microparticles based on resorbable polyesters of oxyalkanoic acids as a platform for deposition and delivery of drugs
Место публикации : Polym. Sci. Ser. A: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2012. - Vol. 54, Is. 2. - С. 94-105. - 12. - ISSN 0965-545X, DOI 10.1134/S0965545X12020022
Примечания : Cited References: 33. - This work was supported by the program for Support of Leading Scientific Schools of the Russian Federation (project no. 11.G34.31.0013.2010, Biotechnology of New Biomaterials) and the program of integrated studies of the Presidium of the Siberian Branch, Russian Academy of Sciences (project no. 93).
Предметные рубрики: IN-VITRO RELEASE
POLYHYDROXYBUTYRATE MICROSPHERES
BLENDS
RIFAMPICIN
BIOCOMPATIBILITY
DEGRADATION
FORMULATION
COMPOSITE
CARRIERS
MODEL
Аннотация: The effect of the preparation technique (chemical composition of a polymer, type and method of emulsion mixing, and molecular mass of a drug) on the yield, structure, and size of microparticles obtained from resorbable polyesters of microbiological origin, polyhydroxyalkanoates, is studied. It is found that the concentration of the polymer solution and the method of emulsion mixing are the most significant factors affecting the diameter of microparticles based on polyhydroxyalkanoates; the surface structure of particles depends to a higher extent on the chemical composition of the polymer. The family of microparticles from 100-200 nm to 50-70 mu m in diameter is synthesized. It is shown that the rate of drug release from microparticles in vitro into the medium is higher in the case of 3-hydroxybutyrate copolymers with 3-hydroxyvalerate than in the case of the homopolymer of 3-hydroxybutyrate. This parameter increases with the content of 3-hydroxyvalerate units in the copolymer and the porosity and mass fraction of the drug in particles with a decrease in their sizes. For in vitro systems containing a phosphate buffer, variation in the preparation parameters makes it possible to obtain microparticles with various characteristics suitable for deposition of drugs. For microparticles obtained from polyhydroxyalkanoates and having different diameters, the mathematical description of the kinetics of drug release from the polymer matrix is provided.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin, Anatoly Nikolayevich, Dvoinina, Ljublyana Mikhailovna, Sukovatyi, Aleksey Grigorievich, Sukhanova, Anna Alekseevna
Заглавие : Production of Porous Films Based on Biodegradable Polyesters by the Casting Solution Technique Using a Co-Soluble Porogen (Camphor)
Колич.характеристики :12 с
Коллективы : Ministry of Science and Higher Education of the Russian Federation [FEFE-2020-0015]
Место публикации : Polymers: MDPI, 2020. - Vol. 12, Is. 9. - Ст.1950. - ISSN 2073-4360(eISSN), DOI 10.3390/polym12091950
Примечания : Cited References:42. - This work was carried out by the team of the scientific laboratory "Smart Materials and Structures" within the state assignment of the Ministry of Science and Higher Education of the Russian Federation for the implementation of the project "Development of multifunctional smart materials and structures based on modified polymer composite materials capable to function in extreme conditions" (Project No. FEFE-2020-0015).
Предметные рубрики: TISSUE ENGINEERING APPLICATIONS
SCAFFOLDS
FABRICATION
POLYMERS
Аннотация: Porous films have been prepared from degradable polymers-poly-3-hydroxybutyrate (PHB), poly-epsilon-caprolactone (PCL) and a blend of these polymers (1:3)-by adding porogen (camphor) to the polymer solution at 10%, 30% or 50% of the total mass of the polymer and porogen, and leaching it out afterwards. After the rinse, camphor content in films decreased to about 0.025%. The structure, physical/mechanical and biological properties of the films were investigated as dependent on their composition and porosity, which varied depending on the amount of camphor added. The surface of PHB films was porous, the PCL films were relatively smooth, and the PHB/PCL films had an intermediate structure. The addition of camphor increased the thickness (from 35 to 45 mu m, from 40 to 80 mu m and from 20 to 65 mu m for PHB, PCL and PHB/PCL, respectively) and porosity (from 4.2(+/- 3.6)% to 50.0(+/- 12.8)%, from 6.4(+/- 5.5)% to 54.5(+/- 6.0)% and from 4.9(+/- 4.8)% to 51.5(+/- 5.8)%, respectively) of the films. The introduction (and removal) of 10% camphor into the PHB and PHB/PCL films led to an approximately twofold increase in the polar component of the free surface energy (from 5.4 +/- 0.38 to 11.8 +/- 1.33 and from 2.7 +/- 0.13 to 5.2 +/- 0.09 mN/m, respectively) but in other cases, on the contrary, a decrease in this indicator was registered. The increase of camphor addition from 0% to 50% gradually impaired mechanical properties of the films: so, Young's modulus decreased from 3.6 to 1.8 GPa, from 0.30 to 0.12 GPa and from 0.50 to 0.20 GPa for PHB, PCL and PHB/PCL, respectively. At the same time, the water vapor transmission rate considerably increased from 197.37 +/- 23.62 to 934.03 +/- 114.34 g/m(2)/d for PHB films; from 1027.99 +/- 154.10 to 7014.62 +/- 280.81 g/m(2)/d for PCL films; and from 715.47 +/- 50.08 to 4239.09 +/- 275.54 g/m(2)/d for PHB/PCL films. Results of biocompatibility testing in the culture of NIH 3T3 mouse fibroblast cells showed that for the most of experimental samples cell adhesion and proliferation were comparable or superior to the corresponding parameters on the initial nonporous films. The best results were obtained for PHB films where at Day 3 of the experiment the registered cell density for experimental samples arrived at 2.66(+/- 0.26) x 10(5) cells/cm(2) versus 1.29(+/- 0.33) x 10(5) cells/cm(2) in the control. So, the proposed method can be used to construct highly porous cell scaffolds for cellular engineering.
WOS
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A. N., Dvoinina L. M., Sukovatyi A. G., Sukhanova A. A.
Заглавие : Production of porous films based on biodegradable polyesters by the casting solution technique using a co-soluble porogen (Camphor)
Место публикации : Polym.: MDPI AG, 2020. - Vol. 12, Is. 9. - Ст.1950. - С. 1-12. - ISSN 20734360 (ISSN), DOI 10.3390/polym12091950
Аннотация: Porous films have been prepared from degradable polymers—poly-3-hydroxybutyrate (PHB), poly-?-caprolactone (PCL) and a blend of these polymers (1:3)—by adding porogen (camphor) to the polymer solution at 10%, 30% or 50% of the total mass of the polymer and porogen, and leaching it out afterwards. After the rinse, camphor content in films decreased to about 0.025%. The structure, physical/mechanical and biological properties of the films were investigated as dependent on their composition and porosity, which varied depending on the amount of camphor added. The surface of PHB films was porous, the PCL films were relatively smooth, and the PHB/PCL films had an intermediate structure. The addition of camphor increased the thickness (from 35 to 45 µm, from 40 to 80 µm and from 20 to 65 µm for PHB, PCL and PHB/PCL, respectively) and porosity (from 4.2(±3.6)% to 50.0(±12.8)%, from 6.4(±5.5)% to 54.5(±6.0)% and from 4.9(±4.8)% to 51.5(±5.8)%, respectively) of the films. The introduction (and removal) of 10% camphor into the PHB and PHB/PCL films led to an approximately twofold increase in the polar component of the free surface energy (from 5.4 ± 0.38 to 11.8 ± 1.33 and from 2.7 ± 0.13 to 5.2 ± 0.09 mN/m, respectively) but in other cases, on the contrary, a decrease in this indicator was registered. The increase of camphor addition from 0% to 50% gradually impaired mechanical properties of the films: so, Young’s modulus decreased from 3.6 to 1.8 GPa, from 0.30 to 0.12 GPa and from 0.50 to 0.20 GPa for PHB, PCL and PHB/PCL, respectively. At the same time, the water vapor transmission rate considerably increased from 197.37 ± 23.62 to 934.03 ± 114.34 g/m2 /d for PHB films; from 1027.99 ± 154.10 to 7014.62 ± 280.81 g/m2 /d for PCL films; and from 715.47 ± 50.08 to 4239.09 ± 275.54 g/m2 /d for PHB/PCL films. Results of biocompatibility testing in the culture of NIH 3T3 mouse fibroblast cells showed that for the most of experimental samples cell adhesion and proliferation were comparable or superior to the corresponding parameters on the initial nonporous films. The best results were obtained for PHB films where at Day 3 of the experiment the registered cell density for experimental samples arrived at 2.66(±0.26) ? 105 cells/cm2 versus 1.29(±0.33) ? 105 cells/cm2 in the control. So, the proposed method can be used to construct highly porous cell scaffolds for cellular engineering. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin, Anatoly Nikolayevich, Sukhanova, Anna Alekseevna, Orlova, Viktoriya Viktorovna, Volchek, Alexander Ivanovich
Заглавие : A novel method of fabricating polymer tubes using the casting solution technique
Колич.характеристики :4 с
Коллективы : scientific laboratory "Intelligent Materials and Structures"within the state assignment of the Ministry of Science and Higher Education of the Russian Federation [FEFE-2020-0015]
Место публикации : Mater. Lett.: ELSEVIER, 2021. - Vol. 282. - Ст.128833. - ISSN 0167-577X, DOI 10.1016/j.matlet.2020.128833. - ISSN 1873-4979(eISSN)
Примечания : Cited References:8. - This work was carried out by the team of the scientific laboratory "Intelligent Materials and Structures"within the state assignment of the Ministry of Science and Higher Education of the Russian Federation for the implementation of the project "Development of multifunctional smart materials and structures based on modified polymer composite materials capable to function in extreme conditions" (Project No. FEFE-2020-0015).
Аннотация: A procedure has been developed to fabricate polymer tubes using solution casting inside template tubes. Polyester solution placed inside the vertically fixed template evaporated forming a hollow tube on the inner walls of the template. Silicone tubes used as the templates were permeable to solvent vapors and had relatively low adhesion to materials of the fabricated tubes, enabling their effortless removal. Parameters of the polymer tubes were determined by a type of the polymer, its concentration in the solution, and the inner diameter of the template. In the first method, a clamp was placed on the lower end of the template tube. A thickness of the new tube walls gradually increased from the open to the clamped end of the template tube. In another method viscous polymer solution without clamping was used; in this case, a polymeric bulkhead was formed in the middle of the tube. Two microbial polyesters, poly-3-hydroxybutyrate and poly-3-hydroxybutyrate-co-3-hydroxyvalerate, and also synthetic poly-L-lactide were used as model polymers. (C) 2020 Elsevier B.V. All rights reserved.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A. N., Kazantseva E. A.
Заглавие : Constructing slow-release formulations of herbicide metribuzin using its co-extrusion with biodegradable polyester poly-ε-caprolactone
Место публикации : J. Environ. Sci. Health Part B Pestic. Food Contamin. Agric. Wastes: Bellwether Publishing, Ltd., 2021. - Article in press. - ISSN 03601234 (ISSN), DOI 10.1080/03601234.2021.1911206
Аннотация: Different technologies to prepare long term pesticide forms include polymer coating, preparing composites and encapsulating pesticides in nanoparticles. A simple and low-cost method was proposed to obtain slow-release formulations by co-extrusion of a pesticide with a biodegradable polymer at a temperature above the melting points of both components. A herbicide metribuzin and low-melting polyester poly-?-caprolactone were chosen for this work. Formulations containing 10%, 20%, and 40% herbicide were prepared. During 7 days of their exposition in water, it was released from 81% to 96% of initially loaded metribuzin; the highest release was detected for 40%-loaded forms. Biodegradation of the constructs and pesticide release were further studied in the model soil. Degradation rates of the specimens increased with an increase in pesticide content, from 9% to 20% over 14 weeks for the 10%/20%-loaded and the 40%-loaded specimens, respectively. The release of metribuzin reached, respectively, 37–38% and 55%. The herbicide content in soil was lower due to its partial degradation in soil; it reached 23–25% and 33%, respectively, from initially loaded into the polymer matrix. Release kinetics of metribuzin in water as in soil best fitted the First-order model. The used approach is promising for obtaining long-term release formulations for soil applications. © 2021 Taylor & Francis Group, LLC.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A. N., Kazantseva E. A.
Заглавие : Constructing Slow-Release Metribuzin Formulations by Co-extrusion of the Pesticide with Poly-?-Caprolactone
Место публикации : Macromol. Sympos.: Wiley-VCH Verlag, 2021. - Vol. 395, Is. 1. - Ст.2000283. - ISSN 10221360 (ISSN), DOI 10.1002/masy.202000283
Аннотация: A simple and low-cost method of obtaining slow-release pesticide formulations is proposed by co-extrusion of a herbicide metribuzin with a low-melting biodegradable polyester poly-?-caprolactone, at a temperature above the melting points of both components. Formulations containing 10%, 20%, and 40% herbicide are prepared. Metribuzin release in water during 7 days of exposition reached 81% from the formulations with the 10% loading and 96% from the specimens with the 40% herbicide loading. Biodegradation and pesticide release from the polymer constructs are studied in the model soil for 14 weeks. Degradation rates of the specimens increased with an increase in pesticide content: between 9% for the 10%-loaded specimen and 20% for the 40%-loaded specimen over 14 weeks. The release of metribuzin from the specimens with the 10–20% and 40% loadings reached 37–38% and 55%, respectively; thus, taking into account soil degradation of the herbicide, the herbicide content in soil reached 23–25% and 33%, respectively, of the initially loaded into the polymer matrix. The used approach is promising to obtain long-term release formulations for soil application. © 2021 Wiley-VCH GmbH
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A. N., Sukhanova A. A., Orlova V. V., Volchek A. I.
Заглавие : A novel method of fabricating polymer tubes using the casting solution technique
Место публикации : Mater Lett: Elsevier B.V., 2021. - Vol. 282. - Ст.128833. - ISSN 0167577X (ISSN), DOI 10.1016/j.matlet.2020.128833
Аннотация: A procedure has been developed to fabricate polymer tubes using solution casting inside template tubes. Polyester solution placed inside the vertically fixed template evaporated forming a hollow tube on the inner walls of the template. Silicone tubes used as the templates were permeable to solvent vapors and had relatively low adhesion to materials of the fabricated tubes, enabling their effortless removal. Parameters of the polymer tubes were determined by a type of the polymer, its concentration in the solution, and the inner diameter of the template. In the first method, a clamp was placed on the lower end of the template tube. A thickness of the new tube walls gradually increased from the open to the clamped end of the template tube. In another method viscous polymer solution without clamping was used; in this case, a polymeric bulkhead was formed in the middle of the tube. Two microbial polyesters, poly-3-hydroxybutyrate and poly-3-hydroxybutyrate-co-3-hydroxyvalerate, and also synthetic poly-L-lactide were used as model polymers. © 2020 Elsevier B.V.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sevastianov V.I., Perova N.V., Shishatskaya E.I., Kalacheva G.S., Volova T.G.
Заглавие : Production of purified polyhydroxyalkanoates (PHAs) for applications in contact with blood
Колич.характеристики :14 с
Место публикации : J. Biomater. Sci.-Polym. Ed.: VSP BV, 2003. - Vol. 14, Is. 10. - P1029-1042. - ISSN 0920-5063, DOI 10.1163/156856203769231547
Примечания : Cited References: 34
Предметные рубрики: BIODEGRADABLE POLYESTERS
POLYMERS
Ключевые слова (''Своб.индексиров.''): polyhydroxyalkanoates (phas)--polyhydroxybutyrate (phb)--poly(hydroxybutyrate-co-hydroxyvalerate) (phbv)--endotoxins--beta-hydroxy acids--hemocompatibility
Аннотация: Samples of olyhydroxyalkanoates (PHAs), polyhydroxybutyrate (PHB) and copolymers poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) with 4 and 18 mol% hydroxyvalerate, synthesized by the bacteria Ralstonia eutropha B5786, were investigated. PHA films in contact with blood did not activate the hemostasis system at the level of cell response, but they did activate the coagulation system and the complement reaction. To detect biologically-active components in the PHAs, a detailed analysis of the composition of the polymers was conducted. Gas chromatography-mass spectrometry revealed long-chain fatty acids (FAs) in the tested PHAs. Their total concentration in the polymer ranged from tenths of mol% to 2-3 mol%, depending on the purification method. C-16:0 constituted the largest proportion, up to 70%. Of the long-chain hydroxy acids, only beta-OH-C-14:0 was detected and it did not exceed 0.06 mol%. The analysis of the hemocompatibility properties of the PHAs purified by a specialized procedure, including the quantitative and morphological estimation of platelets adherent to the surface of polymer films, the plasma recalcification time and complement activation studies, indicated that PHB and PHBV can be used in contact with blood. It has been found out that the lipopolysaccharides of bacteria producing PHAs, which contain mostly long-chain hydroxy acids, can be the factor activating the hemostasis systems. Thus, the technology of PHA purification must satisfy rather stringent specific requirements.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T..., Shishatskaya E..., Sevastianov V..., Efremov S..., Mogilnaya O...
Заглавие : Results of biomedical investigations of PHB and PHB/PHV fibers
Колич.характеристики :9 с
Место публикации : Biochem. Eng. J.: ELSEVIER SCIENCE SA, 2003. - Vol. 16, Is. 2. - P125-133. - ISSN 1369-703X, DOI 10.1016/S1369-703X(03)00038-X
Примечания : Cited References: 20
Предметные рубрики: POLYHYDROXYALKANOATES
POLYESTERS
POLYMERS
Ключевые слова (''Своб.индексиров.''): polyhydroxyalkanoates (phas)--poly-3-hydroxybutyrate (phb)--poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate (phb/phv)--fibers--implants--biocompatibility--tissue reaction
Аннотация: The paper presents the results of biomedical investigations of sutures made of polyhydroxyalkanoates (PHAs) of two types (poly-3-hydroxybutyrate (PHB) and poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate (PHB/PHV)), synthesized by the bacteria Ralstonia eutropha B 5786 in the experiment on test animals in vivo, in comparison with silk and catgut sutures. PHB and PHB/PHV (PHBV) implants produced no adverse effect on physiological, biochemical and functional parameters of the animals during the post-surgery period. The tested PHA sutures featured the necessary strength throughout the healing period of the muscle-fascial cuts. The reaction of tissues to the implantation of PHB and PHB/PHV fibers fitted into the usual scheme characteristic of the wound process and of the reaction to a foreign-body invasion. This reaction and the reaction of tissues to silk had the similar nature and period of inflammation, but it was much less pronounced than the reaction to catgut. The tissue response to the implantation of PHAs consisted in a short-duration (up to 2 weeks) post-traumatic inflammation and the formation of a fibrous capsule less than 200 mum thick during weeks 4-8, which in 4-6 months was reduced to 40-60 mum in the course of reverse development. There were no adverse changes, such as suppurative inflammation, necrosis, calcification, and malignization of the cicatrice, at the site of implantation of PHA filaments, unlike in the cases with silk and catgut. In the case of PHA implantation there was a typical prolonged (throughout the post-surgery monitoring period) pronounced macrophagal stage with a large number of macrophages present. The macrophages were of the phagocytic type and multinucleate giant foreign cells, with a high activity of acid phosphomonoesterase that correlated with the activity of the enzyme in blood. Throughout the period of monitoring no differences in the tissue response to the implantation of the polymer filaments of two PHA types were recorded. (C) 2003 Elsevier Science B.V. All rights reserved.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Plotnikov V.F., Shishatskaya E.I., Mironov P.V., Vasil'ev A.D.
Заглавие : Physicochemical properties of two-component polyhydroxyalkanoates [Poly(3HB/3HV)]
Колич.характеристики :9 с
Место публикации : Biofizika: MEZHDUNARODNAYA KNIGA, 2004. - Vol. 49, Is. 6. - P1038-1046. - ISSN 0006-3029
Примечания : Cited References: 21
Предметные рубрики: BACTERIAL POLYHYDROXYALKANOATES
POLYESTERS
Ключевые слова (''Своб.индексиров.''): polyhydroxyalkanoates--copolymers of hydroxybutyrate and hydroxyvalerate--structure--physicochemical properties
Аннотация: A series of two-component polyhydroxyalkanoates consisting of hydroxybutyrate and hydroxyvalerate monomer at different ratios were synthesized using the bacterium Ralstonia eutropha B5786. The properties of polyhydroxyalkanoates were compared with those of the homopolymer of hydroxybutyric acid by X-ray structure analysis, IR spectroscopy, differential scanning calorimetry, and viscosimetry. With an increase in the molar fraction of hydroxyvalerate, an equalization of the ratio of the crystalline and amorphous phases in the copolymer was observed. The degree of crystallinity of the polymer decreased from 70-80 to 45-50%; in the range of an increase in the hydroxyvalerate molar fraction from several to 25-30 mol %, the dependence was linear. The temperature characteristics, the melting temperature (T-m), and the degradation temperature (T-d) were lower in polyhydroxyalkanoates than in polyhydroxybutyrate, for which T-m and T-d were 168-170 and 260-265degreesC, respectively. In the copolymer, as the molar fraction of hydroxyvalerate grew, both parameters decreased. In the range of variation of monomer ratio studied, they decreased to 150-160 and 200-220degreesC, respectively. No distinct correlation between the composition of the polymer and its molecular mass was found.
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