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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Prudnikova S.V., Karpov V.A., Ivonin V.N., D? N.L., Nguy?n T.H., Le T.M.H., Filichev N.L., Levin A.L., Filipenko M.L., Volova T.G., Gitelson I.I.
Заглавие : Microbial degradation of polyhydroxyalkanoates in tropical soils
Место публикации : International Biodeterioration and Biodegradation. - 2013. - Vol. 83. - С. 77-84. - ISSN 09648305 (ISSN) , DOI 10.1016/j.ibiod.2013.04.014
Ключевые слова (''Своб.индексиров.''): biopolymer properties--biopolymers--pha degrading microorganisms--polyhydroxyalkanoates (phas)--soil biodegradation--3-hydroxybutyric acid--degree of crystallinity--hydroxyalkanoic acids--microbial characteristics--microbial degradation--polyhydroxyalkanoates--polymer molecular mass--soil biodegradations--bacteriology--biomolecules--biopolymers--microorganisms--soils--tropics--biodegradation--biodegradation--chemical composition--microbial activity--microbial community--molecular analysis--organic compound--polymer--soil degradation--soil microorganism--tropical soil
Аннотация: The integrated study addressing biodegradation of microbial linear polyesters of hydroxyalkanoic acids (polyhydroxyalkanoates, PHAs) in tropical conditions by microbial communities of Vietnamese soils was performed in locations close to Hanoi and Nha Trang, which differed in their weather conditions and microbial communities. It shows that PHA degradation in tropical soils is influenced by polymer chemical composition, specimen shape, and microbial characteristics. The homopolymer of 3-hydroxybutyric acid is degraded at higher rates than the copolymer of 3-hydroxybutyric and 3-hydroxyvaleric acids. The average rates of mass loss were 0.04-0.33% per day for films and 0.02-0.18% for compact pellets. PHA degradation was accompanied by a decrease in the polymer molecular mass and, usually, an increase in the degree of crystallinity, suggesting preferential degradation of the amorphous phase. Under the study conditions, representatives of the bacterial genera Burkholderia, Bacillus, Cupriavidus, Mycobacterium, and Nocardiopsis and such micromycetes as Acremonium, Gongronella, Paecilomyces, and Penicillium, Trichoderma have been identified as major PHA degraders. В© 2013 Elsevier Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Zhila N.O., Kalacheva G.S., Brigham C.J., Sinskey A.J.
Заглавие : Effects of intracellular poly(3-hydroxybutyrate) reserves on physiological-biochemical properties and growth of Ralstonia eutropha
Место публикации : Research in Microbiology. - 2013. - Vol. 164, Is. 2. - С. 164-171. - ISSN 09232508 (ISSN) , DOI 10.1016/j.resmic.2012.10.008
Ключевые слова (''Своб.индексиров.''): granules--pha cycle--poly(3-hydroxybutyrate)--ralstonia eutropha--poly(3 hydroxybutyric acid)--article--bacterial growth--bacterial strain--bacterium culture--cell division--cell granule--cell size--controlled study--cupriavidus necator--electron microscopy--nonhuman--polymer production--priority journal--cupriavidus necator--cytoplasmic granules--hydroxybutyrates--microscopy, electron, transmission--polyesters--bacteria (microorganisms)--cupriavidus necator
Аннотация: Microbial polyhydroxyalkanoates (PHAs), because of their well studied complex physiology and commercial potential, are vehicles for carbon and potential storage reduction for many microbial species. Even with the wealth of studies about microbial PHAs in the scientific literature, polymer accumulation and degradation are still not comprehensively understood. Poly(3-hydroxybutyrate) (P3HB) granule formation and polymer mobility were studied here in the bacterium Ralstonia eutropha strain B5786 in autotrophic cultures. Electron microscopy studies revealed decreasing cell size concomitant with enlargement of size and number of intracellular granules, and inhibition of cell division during intracellular polymer production. Activities of key P3HB biosynthetic enzymes demonstrated correlations with each other during polymer accumulation, suggesting an intricately regulated P3HB cycle in autotrophically grown R. eutropha cells. В© 2012 .
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Voinova O.N., Kalacheva G.S., Grodnitskaya I.D., Volova T.G.
Заглавие : Microbial polymers as a degradable carrier for pesticide delivery
Место публикации : Applied Biochemistry and Microbiology. - 2009. - Vol. 45, Is. 4. - С. 384-388. - ISSN 00036838 (ISSN) , DOI 10.1134/S0003683809040061
Аннотация: The possibility of use of polyhydroxyalkanoates (PHAs), biodegradable microbial polyesters, as a carrier for pesticides (?-hexachlorcyclohexane and lindane) for targeted and controlled delivery of these compounds to soil was investigated. The kinetics of polymer degradation and the dynamics of pesticide release from the extended-release formulations was studied. It is shown that pesticides embedded in a degradable polymer (PHA) carrier are released gradually and slowly, without surges, as the polymer is degraded by the soil micro-flora. The microbial soil component actively responded to the addition of the polymer as an additional nutrient substrate: the latter was degraded and then utilized. The rate of the pesticide release to the soil can be regulated by varying the polymer-pesticide ratio. В© 2009 Pleiades Publishing, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Goreva A.V., Voinova O.N., Inzhevatkin E.V., Khlebopros R.G., Volova T.G.
Заглавие : Evaluation of antitumor activity of rubomycin deposited in absorbable polymeric microparticles
Место публикации : Bulletin of Experimental Biology and Medicine. - 2008. - Vol. 145, Is. 3. - С. 358-361. - ISSN 00074888 (ISSN) , DOI 10.1007/s10517-008-0091-9
Ключевые слова (''Своб.индексиров.''): absorbable polymers--ehrlich's ascitic carcinoma--microencapsulation--polyhydroxybutyrate--rubomycin--poly(3 hydroxybutyric acid)--polymer--rubomycin--animal cell--animal experiment--animal model--antineoplastic activity--article--bagg albino mouse--cancer inhibition--cancer mortality--cancer survival--controlled study--drug delivery system--ehrlich ascites tumor--microencapsulation--mouse--nonhuman--polymerization--survival rate--tumor cell--tumor volume--absorption--animals--antibiotics, antineoplastic--carcinoma, ehrlich tumor--daunorubicin--hydroxybutyrates--mice--mice, inbred balb c--microspheres--polyesters--mus
Аннотация: An experimental dosage form of rubomycin is developed: the drug is incorporated in absorbable polymeric (polyhydroxybutyrate) matrix in the form of microparticles. Antitumor efficiency of this rubomycin dosage form was studied in laboratory mice with transplanted Ehrlich ascitic carcinoma. Rubomycin deposited in polymeric microparticles exhibited pronounced antitumor activity, inhibited the proliferative activity of Ehrlich ascitic carcinoma, and improved survival of mice with tumors. This dosage form of the drug can be used for local injections. В© Springer Science+Business Media, Inc. 2008.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Voinova O., Gladyshev M., Volova T.G.
Заглавие : Comparative study of PHA degradation in natural reservoirs having various types of ecosystems
Место публикации : Macromolecular Symposia. - 2008. - Vol. 269, Is. 1. - С. 34-37. - ISSN 10221360 (ISSN) , DOI 10.1002/masy.200850906
Ключевые слова (''Своб.индексиров.''): biodegradable polyesters--ecosystem--natural environment--pha--water reservoir--abs resins--biochemistry--biodegradable polymers--biodegradation--biopolymers--biotechnology--degradation--ecology--ecosystems--esters--polymers--aerobic and anaerobic conditions--anaerobic conditions--biodegradable polyesters--comparative studies--ecological characteristics--environment temperatures--natural environment--natural environments--pha--polyhydroxyalkanoate--polymer degradations--water reservoir--water reservoirs--reservoirs (water)
Аннотация: The kinetics of polyhydroxyalkanoate (PHA) degradation in natural environment in two water reservoirs having various ecological characteristics was studied. It was shown that biodegradation of polymer essentially depends on the environment temperature and inorganic composition of water. The processes of polymer degradation under aerobic and anaerobic conditions were compared. The polymer degradation was slower under anaerobic conditions. Copyright В© 2008 WILEY-VCH Verlag GmbH & Co. KGaA.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A., Kalacheva G.S., Medvedeva S., Rodicheva E., Volova T.G.
Заглавие : Luminous bacteria as producers of polyhydroxyalkanoates
Место публикации : Macromolecular Symposia. - 2008. - Vol. 269, Is. 1. - С. 17-22. - ISSN 10221360 (ISSN) , DOI 10.1002/masy.200850904
Ключевые слова (''Своб.индексиров.''): luminous bacteria--polyhydroxyalkanoates--polyhydroxybutyrate--abs resins--acids--bacteriology--batch cell culture--biological materials--biomass--biopolymers--biotechnology--cell culture--esters--hydrocarbons--organic compounds--polymers--renewable energy resources--supramolecular chemistry--batch cultures--dry cells--luminous bacteria--micro-organisms--photobacterium leiognathi--photobacterium phosphoreum--polyhydroxyalkanoates--polyhydroxybutyrate--polymer yields--vibrio fischeri--bioluminescence
Аннотация: The study addresses the ability of luminous bacteria of different taxa (Photobacterium leiognathi, Photobacterium phosphoreum, Vibrio harveyi, Vibrio fischeri) to synthesize polyesters of hydrocarbon acids (polyhydroxyalkanoates, PHAs) as storage macromolecules. The screened strains widely varied in their PHA productivity. Conditions for attaining high polymer yields (including two- and three-component polymers) in batch culture have been determined. The attained polymer yields reached 40-70% of dry cell biomass. The results suggest a conclusion that luminous microorganisms can be considered as producers of multi-component PHAs. Copyright В© 2008 WILEY-VCH Verlag GmbH & Co. KGaA.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Kalacheva G.S., Rodicheva E.K., Volova T.G.
Заглавие : Synthesis of reserve polyhydroxyalkanoates by luminescent bacteria
Место публикации : Microbiology. - 2008. - Vol. 77, Is. 3. - С. 318-323. - ISSN 00262617 (ISSN) , DOI 10.1134/S0026261708030119
Ключевые слова (''Своб.индексиров.''): biosynthesis--chemical structure--luminescent bacteria--polyhydroxyalkanoates (pha)--bacteria (microorganisms)--photobacterium leiognathi--vibrio harveyi
Аннотация: The ability of marine luminescent bacteria to synthesize polyesters of hydroxycarboxylic acids (polyhydroxyalkanoates, PHA) as reserve macromolecules was studied. Twenty strains from the collection of the luminescent bacteria CCIBSO (WDCM839) of the Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, assigned to different taxa (Photobacterium leiognathi, Ph. phosphoreum, Vibrio harveyi, and V. fischeri) were analyzed. The most productive strains were identified, and the conditions ensuring high polymer yields in batch culture (40-70% of the cell dry mass weight) were determined. The capacity for synthesizing two-and three-component polymers containing hydroxybutyric acid as the main monomer and hydroxyvaleric and hydroxyhexanoic acids was revealed in Ph. leiognathi and V. harveyi strains. The results allow luminescent microorganisms to be regarded as new producers of multicomponent polyhydroxyalkanoates. В© 2008 MAIK Nauka.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Voinova O.N., Kalacheva G.S., Grodnitskaya I.D.
Заглавие : The prospects of the use of resorbable polyesters for designing safe pesticides
Место публикации : Doklady Biological Sciences. - 2008. - Vol. 419, Is. 1. - С. 100-103. - ISSN 00124966 (ISSN) , DOI 10.1134/S0012496608020099
Ключевые слова (''Своб.индексиров.''): pesticide--polyester--article--chemistry--drug design--metabolism--microbiology--drug design--pesticides--polyesters--soil microbiology
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Voinova O.N., Goreva A.V., Mogilnaya O.A., Volova T.G.
Заглавие : Tissue reaction to intramuscular injection of resorbable polymer microparticles
Место публикации : Bulletin of Experimental Biology and Medicine. - 2007. - Vol. 144, Is. 6. - С. 786-790. - ISSN 00074888 (ISSN) , DOI 10.1007/s10517-007-0432-0
Ключевые слова (''Своб.индексиров.''): microencapsulation--polyhydroxybutyrate--resorbable polymers--tissue reaction--poly(3 hydroxybutyric acid)--polymer--animal experiment--animal tissue--article--cell infiltration--controlled study--drug delivery system--drug formulation--female--giant cell--inflammation--macrophage--microencapsulation--nonhuman--rat--tissue reaction--absorbable implants--animals--drug compounding--female--foreign-body reaction--injections, intramuscular--microspheres--polyesters--rats--rats, wistar
Аннотация: Tissue reaction to implantation of polymeric microparticles from resorbable polymer (polyhydroxybutyrate) is characterized by slight inflammatory reaction and pronounced progressive macrophage infiltration with the presence of mono-and multinuclear foreign body giant cells resorbing the polymeric matrix. No fibrous capsules were formed around the polymeric microparticles; neither necrosis nor other adverse morphological changes and tissue transformation in response to implantation of the PHB microparticles were recorded. The results indicate good prospects of using polyhydroxybutyrate for the construction of long-acting dosage forms as microparticles for intramuscular injection. В© Springer Science+Business Media, Inc. 2007.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Kalacheva G.S., Rodicheva E.K., Volova T.G.
Заглавие : Luminous bacteria as potential producers of resorbed polyhydroxyalkanoate polyesters
Место публикации : Doklady Biochemistry and Biophysics. - 2007. - Vol. 416, Is. 1-6. - С. 248-251. - ISSN 16076729 (ISSN) , DOI 10.1134/S1607672907050067
Ключевые слова (''Своб.индексиров.''): photoprotein--polyester--polyhydroxyalkanoic acid--article--bacterium--chemistry--chemoluminescence--classification--genetic procedures--genetics--metabolism--photobacterium--phylogeny--time--bacteria--biosensing techniques--chemiluminescent measurements--luminescent proteins--photobacterium--phylogeny--polyesters--polyhydroxyalkanoates--time factors--bacteria (microorganisms)
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Trusova M.Y., Kalacheva G.S., Kozhevnicov I.V.
Заглавие : Physiological-biochemical properties and the ability to synthesize polyhydroxyalkanoates of the glucose-utilizing strain of the hydrogen bacterium Ralstonia eutropha B8562
Место публикации : Applied Microbiology and Biotechnology. - 2006. - Vol. 73, Is. 3. - С. 429-433. - ISSN 01757598 (ISSN) , DOI 10.1007/s00253-006-0460-0
Ключевые слова (''Своб.индексиров.''): biochemistry--carbon--genetic engineering--glucose--hydrogen--polyesters--cultural properties--glucose-utilizing mutant strain--nitrogen deficiency--polyhydroxyalkanoate (pha)--bacteria--3 hydroxybutyric acid--carbon dioxide--fructose--glucose--hydrogen--monomer--polyhydroxyalkanoic acid--sugar--bacterium--carbon--comparative study--energy--ester--experimental study--glucose--hydrogen--physicochemical property--article--bacterial strain--bacterium culture--bacterium mutant--biomass--chemical analysis--cupriavidus necator--energy consumption--genetic analysis--nonhuman--synthesis--bacteria (microorganisms)--cupriavidus necator
Аннотация: Physiological-biochemical, genetic, and cultural properties of the glucose-utilizing mutant strain Ralstonia eutropha B8562 have been compared with those of its parent strain R. eutropha B5786. It has been shown that growth characteristics of the strain cultured on glucose as the sole carbon and energy source are comparable with those of the parent strain. Strain B8562 is characterized by high polyhydroxyalkanoate (PHA) yields on different carbon sources (CO2, fructose, and glucose). PHA accumulation in the strain batch cultured on glucose under nitrogen deficiency reaches 90 %. The major monomer in the PHA is ?-hydroxybutyric acid (more than 99 mol %); the identified minor components are ?-hydroxyvaleric acid (0.25-0.72 mol %) and ?-hydroxyhexanoic acid (0.08-1.5 mol %). The strain is a promising PHA producer on available sugar-containing media with glucose. В© Springer-Verlag 2006.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S.
Заглавие : The synthesis of hydroxybutyrate and hydroxyvalerate copolymers by the bacterium Ralstonia eutropha
Место публикации : Mikrobiologiya. - 2005. - Vol. 74, Is. 1. - С. 63-69. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): ?-ketothiolase--controlled synthesis--poly(hydroxybutyrate-co-hydroxyvalerate)--ralstonia eutropha--bacteria (microorganisms)--cupriavidus necator--acetoacetyl coenzyme a--acetyl coenzyme a acyltransferase--acyl coenzyme a--acyltransferase--butyric acid derivative--carbon dioxide--fructose--hydrogen--poly(3 hydroxybutyrate) co (3 hydroxyvalerate)--poly(3-hydroxyalkanoic acid) synthase--poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate)--polyester--polyhydroxyalkanoate synthase--valeric acid derivative--article--chemistry--crystallization--culture medium--metabolism--wautersia eutropha--acetyl-coa c-acyltransferase--acyl coenzyme a--acyltransferases--butyrates--carbon dioxide--crystallization--culture media--cupriavidus necator--fructose--hydrogen--polyesters--valerates
Аннотация: The paper deals with the study of the synthesis of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) copolymers by the bacterium Ralstonia eutropha B-5786 grown under different carbon nutrition conditions (growth on carbon dioxide, fructose, and CO2-valerate and fructose-valerate mixtures). The parameters to be analyzed included the yield of biomass, the yield, synthesis rate, and composition of copolymers, the activity of the key enzymes of polyhydroxyalkanoate (PHA) synthesis (?-ketothiolase, acetoacetyl-CoA reductase, and PHA synthase), the maximum tolerable concentration of valerate to the bacterium, and the conditions that govern the incorporation of hydroxyvalerate to copolymers. This allowed the relationship between cultivation conditions and the proportion of monomers in the copolymers to be deduced. We were able to synthesize a range of 3HB/3HV copolymers and found that the thermal characteristics and the degree of crystallinity of these copolymers depend on the molar fraction of 3HV.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Gordeev S.A., Puzyr A.P.
Заглавие : Degradation of P(3HB) and P(3HB-co-3HV) in biological media
Место публикации : Journal of Biomaterials Science, Polymer Edition. - 2005. - Vol. 16, Is. 5. - С. 643-657. - ISSN 09205063 (ISSN) , DOI 10.1163/1568562053783678
Ключевые слова (''Своб.индексиров.''): degradation rate--fiber properties--morphology--poly(hydroxybutyrate-co-hydroxyvalerate) (p(3hb-co-3hv))--polyhydroxyalkanoates(phas)--polyhydroxybutyrate (p(3hb))--copolymers--degradation--differential scanning calorimetry--enzymes--morphology--scanning electron microscopy--tensile strength--tissue--transmission electron microscopy--degradation rate--fiber properties--polyhydroxyalkanoates (phas)--polyhydroxybutyrate (p(3hb))--biopolymers--buffer--copolymer--poly(3 hydroxybutyric acid)--polyhydroxybutyrate hydroxyvalerate copolymer--unclassified drug--animal experiment--animal model--animal tissue--article--biodegradation--controlled study--crystal structure--fiber--giant cell--in vitro study--in vivo study--macrophage--morphology--nonhuman--ph--priority journal--rat--structure analysis--tensile strength--tissue water--weight reduction--animals--biodegradation, environmental--buffers--humans--hydrogen-ion concentration--hydroxybutyrates--macrophages--microscopy, electron, scanning--microscopy, electron, transmission--muscle, skeletal--polyesters--rats--rats, wistar
Аннотация: The biodegradability of oriented fibers made of polyhydroxybutyrate (P(3HB)) and its co-polymer with ?-hydroxyvalerate (P(3HB-co-3HV)) was investigated in buffer solutions and in biological media in vitro and in vivo. The fibers of both polymer types demonstrated resistance to hydrolytic degradation in buffer solutions at 38В°C and pH from 4.5 to 7.0 (for up to 180 days). It has been found that the biodegradation of the fibers in vitro in blood and serum and in vivo is accompanied by weight losses and minor changes in the microstructure with no significant losses in the tensile strength over a long time (up to 180 days). The biodegradation rate of the less crystalline co-polymer P(3HB-co-3HV) fibers was 1.4-2.0-times higher than that of the homopolymer P(3HB). It has also been shown that the degradation of the fibers in vivo is influenced both by tissue fluid enzymes and cells (macrophages and foreign-body giant cells). The fibers were eroded on the surface only with no gross defects and no dramatic effects on their mechanical performance. В© VSP 2005.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Puzyr A.P., Mogilnaya O.A., Efremov S.N.
Заглавие : Tissue response to the implantation of biodegradable polyhydroxyalkanoate sutures
Место публикации : Journal of Materials Science: Materials in Medicine. - 2004. - Vol. 15, Is. 6. - С. 719-728. - ISSN 09574530 (ISSN) , DOI 10.1023/B:JMSM.0000030215.49991.0d
Ключевые слова (''Своб.индексиров.''): necrosis--polymeric implants--suppurative inflammation--tissue reaction--biodegradation--calcification (biochemistry)--cells--copolymers--implants (surgical)--silk--tissue--tumors--materials science--acid phosphatase--copolymer--poly(3 hydroxybutyric acid)--polyhydroxyalkanoic acid--polyhydroxyvaleric acid--unclassified drug--animal experiment--animal model--article--biodegradable implant--blood vessel reactivity--catgut--controlled study--enzyme activity--female--giant cell--histochemistry--inflammation--macrophage--nonhuman--priority journal--rat--silk--suture--tensile strength--tissue reaction--tissue structure--wound healing--young modulus--absorbable implants--animals--female--fibrosis--foreign-body reaction--hydroxybutyrates--muscle, skeletal--polyesters--polymers--rats--rats, wistar--sutures--treatment outcome--wounds, penetrating--animalia
Аннотация: Polyhydroxyalkanoate (PHA) sutures were implanted to test animals intramuscularly, and tissue reaction was investigated and compared with the reaction to silk and catgut. Tested monofilament sutures made of PHAs of two types polyhydroxybutyrate (PHB) and a copolymer of hydroxybutyrate and hydroxyvalerate (PHV) featured the strength necessary for the healing of muscle-fascial wounds. The reaction of tissues to polymeric implants was similar to their reaction to silk and was less pronounced than the reaction to catgut; it was expressed in a transient post-traumatic inflammation (up to four weeks) and the formation of a fibrous capsule less than 200 ?m thick, which became as thin as 4060 ?m after 16 weeks, in the course of reverse development. Macrophages and foreign-body giant cells with a high activity of acid phosphatase were actively involved in this process. PHB and PHB/PHV sutures implanted intramuscularly for an extended period (up to one year) did not cause any acute vascular reaction at the site of implantation or any adverse events, such as suppurative inflammation, necrosis, calcification of the fibrous capsule or malignant tumor formation. No statistically significant differences were revealed in the tissue response to polymer sutures of the two types. Capsules around silk and catgut sutures did not become significantly thinner.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Voinov N.A.
Заглавие : Study of Ralstonia eutropha culture producing polyhydroxyalkanoates on products of coal processing
Место публикации : Prikladnaia biokhimiia i mikrobiologiia. - 2004. - Vol. 40, Is. 3. - С. 296-300. - ISSN 05551099 (ISSN)
Ключевые слова (''Своб.индексиров.''): carbon monoxide--coal--polyester--antibiotic resistance--article--culture medium--enzyme specificity--gas--growth, development and aging--metabolism--microbiology--wautersia eutropha--carbon monoxide--coal--culture media--cupriavidus necator--drug resistance, microbial--gases--polyesters--substrate specificity
Аннотация: Kinetic indices of growth, polyhydroxyalkanoate (PHA) accumulation, and gas exchange have been studied in a culture of the carbon monoxide-resistant hydrogen strain Ralstonia eutropha B-5786 grown on a gaseous substrate (GS) obtained by lignite gasification. The GS was shown to be suitable for PHA production. To increase the degree of GS consumption, various modes of gas supply to the culture were tested. Based on the results, an algorithm was developed for calculating and controlling gas-exchange parameters in the PHA-accumulating culture of Ralstonia eutropha, grown on a new GS allowing high polymer yields (up to 75%) and degrees of the substrate utilization (up to 90%).
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sevastianov V.I., Perova N.V., Shishatskaya E.I., Kalacheva G.S., Volova T.G.
Заглавие : Production of purified polyhydroxyalkanoates (PHAs) for applications in contact with blood
Место публикации : Journal of Biomaterials Science, Polymer Edition. - 2003. - Vol. 14, Is. 10. - С. 1029-1042. - ISSN 09205063 (ISSN) , DOI 10.1163/156856203769231547
Ключевые слова (''Своб.индексиров.''): ?-hydroxy acids--endotoxins--hemocompatibility--poly(hydroxybutyrate-co-hydroxyvalerate) (phbv)--polyhydroxyalkanoates (phas)--polyhydroxybutyrate (phb)--bacterium lipopolysaccharide--carbon--complement--copolymer--hydroxyacid--long chain fatty acid--poly(3 hydroxybutyric acid)--polyhydroxyalkanoic acid--valeric acid derivative--adult--article--biofilm--biotechnology--blood analysis--blood clotting--blood compatibility--cell function--chemical analysis--chemical composition--complement activation--concentration (parameters)--controlled study--gas chromatography--hemostasis--human--human cell--mass spectrometry--micromorphology--nonhuman--priority journal--purification--quantitative analysis--sampling--synthesis--thrombocyte adhesion--wautersia eutropha--biocompatible materials--blood--blood coagulation tests--chromatography, gas--complement activation--cupriavidus necator--fatty acids--humans--platelet adhesiveness--polyesters--surface properties
Аннотация: Samples of olyhydroxyalkanoates (PHAs), polyhydroxybutyrate (PHB) and copolymers poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) with 4 and 18 mol% hydroxyvalerate, synthesized by the bacteria Ralstonia eutropha B5786, were investigated. PHA films in contact with blood did not activate the hemostasis system at the level of cell response, but they did activate the coagulation system and the complement reaction. To detect biologically-active components in the PHAs, a detailed analysis of the composition of the polymers was conducted. Gas chromatography-mass spectrometry revealed long-chain fatty acids (FAs) in the tested PHAs. Their total concentration in the polymer ranged from tenths of mol% to 2-3 mol%, depending on the purification method. C16:0 constituted the largest proportion, up to 70%. Of the long-chain hydroxy acids, only ?-OH-C14:0 was detected and it did not exceed 0.06 mol%. The analysis of the hemocompatibility properties of the PHAs purified by a specialized procedure, including the quantitative and morphological estimation of platelets adherent to the surface of polymer films, the plasma recalcification time and complement activation studies, indicated that PHB and PHBV can be used in contact with blood. It has been found out that the lipopolysaccharides of bacteria producing PHAs, which contain mostly long-chain hydroxy acids, can be the factor activating the hemostasis systems. Thus, the technology of PHA purification must satisfy rather stringent specific requirements.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Voinov N.A.
Заглавие : Kinetic parameters of a culture of the hydrogen-oxidizing Ralstonia eutropha, grown under the regimen of biosynthesis of polyhydroxybutyrate
Место публикации : Prikladnaia biokhimiia i mikrobiologiia. - 2003. - Vol. 39, Is. 2. - С. 189-193. - ISSN 05551099 (ISSN)
Ключевые слова (''Своб.индексиров.''): hydrogen--hydroxybutyric acid--polyester--article--culture medium--enzyme specificity--growth, development and aging--kinetics--metabolism--oxidation reduction reaction--temperature--wautersia eutropha--culture media--cupriavidus necator--hydrogen--hydroxybutyrates--kinetics--oxidation-reduction--polyesters--substrate specificity--temperature
Аннотация: Kinetic parameters of a culture of the hydrogen-oxidizing bacterium Ralstonia eutropha, grown on a gas substrate under the conditions favoring autotrophic biosynthesis of polyhydroxybutyrate, were studied. The following parameters, making it possible to control and optimize the process in industrial situations, were determined: specific rate of substrate consumption, physical properties of culture medium, and coefficients of heat emission and mass transfer.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Altukhova O.V.
Заглавие : Autotrophic synthesis of polyhydroxyalkanoates by the bacteria Ralstonia eutropha in the presence of carbon monoxide
Место публикации : Applied Microbiology and Biotechnology. - 2002. - Vol. 58, Is. 5. - С. 675-678. - ISSN 01757598 (ISSN) , DOI 10.1007/s00253-002-0941-8
Ключевые слова (''Своб.индексиров.''): 3 hydroxybutyric acid--acetoacetyl coenzyme a reductase--acetyl coenzyme a acyltransferase--beta hydroxyvalerate--butyrate dehydrogenase--carbon monoxide--electrolyte--hydrogen--oxidoreductase--poly(3 hydroxybutyric acid)--poly(3 hydroxybutyric acid)synthase--polyhydroxyalkanoic acid--polymer--unclassified drug--valeric acid--bacterium--article--autotrophy--bacterial growth--bacterial strain--biomass production--controlled study--crystallization--enzyme activity--molecular weight--nonhuman--synthesis--temperature--wautersia eutropha--carbon monoxide--culture media--cupriavidus necator--fatty acids--lipids--polyesters--bacteria (microorganisms)--negibacteria--ralstonia--wautersia eutropha
Аннотация: It has been found that the carbon monoxide (CO)-resistant strain of the hydrogen bacteria Ralstonia eutropha B5786 is able to synthesise polyhydroxy-alkanoates (PHAs) in the presence of CO under autotrophic conditions. This strain, grown on model gas mixtures containing 5-25% CO (v/v), accumulates up to 70-75% (of absolutely dry matter) PHA, without significant variation in the yield coefficient on hydrogen. No suppression of the activities of the key enzymes of PHA synthesis (?-ketothiolase, acetoacetyl-CoA-reductase, butyrate dehydrogenase and poly-3-hydroxybutyrate synthase) was recorded. The PHA synthesised is a copolymer containing mostly ?-hydroxybutyrate (more than 99 mol%) with trace amounts of ?-hydroxyvalerate. The investigated properties of the polymer (molecular weight, crystallinity, temperature characteristics) do not differ from those of the polymer synthesised on electrolytic hydrogen.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Altukhova O.V.
Заглавие : The autotrophic synthesis of polyhydroxyalkanoate by alcaligenes eutrophus in the presence of carbon monoxide
Место публикации : Mikrobiologiya. - 2001. - Vol. 70, Is. 6. - С. 745-752. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): co inhibition--hydrogen-oxidizing bacteria--polyhydroxyalkanoates (phas)--synthesis--alkane--carbon monoxide--fatty acid--hydroxyacid--lipid--nitrogen--polyester--article--chemistry--culture medium--drug antagonism--growth, development and aging--mass spectrometry--metabolism--wautersia eutropha--alkanes--carbon monoxide--culture media--cupriavidus necator--fatty acids--hydroxy acids--lipids--mass spectrometry--nitrogen--polyesters
Аннотация: The CO-resistant strain B5786 of the hydrogen-oxidizing bacterium Alcaligenes eutrophus was found to be able to synthesize polyhydroxyalkanoates (PHAs) under the conditions of growth limitation by nitrogen deficiency (the factor that promotes PHA synthesis) and growth inhibition by carbon monoxide. The gas mixtures that contained from 5 to 20 vol % CO did not inhibit the key enzymes of PHA synthesis-?-ketothiolase, acetoacetyl-CoA reductase, hydroxybutyrate dehydrogenase, and PHA synthase. In the presence of CO, cells accumulated up to 70-75 wt % PHA (with respect to the dry biomass) without any noticeable increase in the consumption of the gas substrate. Chromatographic-mass spectrometric analysis showed that the PHA synthesized by A. eutrophus is a copolymer containing more than 99 mol % ?-hydroxybutyrate and trace amounts of ?-hydroxyvalerate. The PHA synthesized under the conditions described did not differ from that synthesized by A. eutrophus cells from electrolytic hydrogen.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Eremeev A.V., Gitel'zon I.I., Setkov N.A., Volova T.G.
Заглавие : Cytotoxicity of polyhydroxyalkanoates in animal cell cultures.
Место публикации : Doklady Biological Sciences. - 2000. - Vol. 374, Is. 1-6. - С. 539-542. - ISSN 00124966 (ISSN)
Ключевые слова (''Своб.индексиров.''): dna--hydroxybutyric acid--poly(3 hydroxybutyrate) co (3 hydroxyvalerate)--poly(3 hydroxybutyric acid)--poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate)--poly-beta-hydroxybutyrate--polyester--rna--thymidine--uridine--animal--article--biosynthesis--cell division--cell strain 3t3--cell survival--cytology--drug effect--liver--liver cell--metabolism--mouse--vascular endothelium--3t3 cells--animals--cell division--cell survival--dna--endothelium, vascular--hepatocytes--hydroxybutyrates--liver--mice--polyesters--rna--thymidine--uridine
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