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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Хижняк С.В., Таушева И.В., Березикова А.А., Нестеренко Е.В., Рогозин Д.Ю.
Заглавие : Психрофильные и психротолерантные гетеротрофные микроорганизмы карстовых полостей Средней Сибири : научное издание
Место публикации : Экология. - 2003. - N 4. - С. 261-266. - ISSN 0367-0597
ГРНТИ : 62.99.29
Предметные рубрики: ПСИХРОФИЛЬНЫЕ МИКРООРГАНИЗМЫ
ПСИХРОТОЛЕРАНТНЫЕ МИКРООРГАНИЗМЫ
ЧИСЛЕННОСТЬ
ИДЕНТИФИКАЦИЯ
ПОДЗЕМНЫЕ ЭКОСИСТЕМЫ
ПЕЩЕРЫ
Аннотация: Исследовали естественную микрофлору карстовых полостей Средней Сибири, представленную психрофильными и психротолерантными бактериями и грибами, способными к росту при т-ре +3...+15'ГРАДУС'C и +3...+28'ГРАДУС'C соответственно. Бактерии в пещерах распространены повсеместно, их численность колеблется от 10{3} до 10{7} клеток/г грунта. Идентификация показала наличие родов Pseudomonas, Arthrobacter, Bacillus и коринебактерий. Грибы встречаются в местах с повышенной антропогенной нагрузкой, их численность достигает 10{6}-10{7} колониеобразующих единиц/г грунта. Среди грибов доминируют представители родов Mucor, Penicillium, Chrysosporium. Россия, Красноярский гос. аграрный ун-т, Красноярск. Библ. 12
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Письман Т.И., Сомова Л.А., Печуркин Н.С.
Заглавие : Математическая модель взаимодействия звеньев в системе растение-ризосферные микроорганизмы при повышенном содержании диоксида углерода в атмосфере : научное издание
Место публикации : Биофизика. - 2002. - Т. 47, N 5. - С. 920-925. - ISSN 0006-3029
ГРНТИ : 34.03.23
Предметные рубрики: ЭКОСИСТЕМЫ
ДИОКСИД УГЛЕРОДА
ВЛИЯНИЕ
РАСТЕНИЯ
РИЗОСФЕРНЫЕ МИКРООРГАНИЗМЫ
МАТЕМАТИЧЕСКАЯ МОДЕЛЬ
Аннотация: Построена математическая модель, описывающая взаимодействие растений и ризосферных микроорганизмов на полной минеральной среде при повышенном содержании CO[2] в атмосфере. Показано положительное действие повышенного содержания CO[2] на систему растение - ризосферные микроорганизмы. С помощью модели продемонстрировано влияние ризосферных микроорганизмов на рост растений. Показано, что биомасса растений в системе растение - ризосферные микроорганизмы меньше биомассы растений без микроорганизмов. На примере простой экосистемы пшеница - Pseudomonas putida - искусственная почва экспериментально подтверждено, что на минеральной среде (без лимита по азоту) при повышенном содержании CO[2] (0,66%) биомасса растений, корни которых инокулированы микроорганизмами, меньше биомассы растений без инокуляции микроорганизмами. Россия, Ин-т биофизики СО РАН, 660036, Красноярск, Академгородок. Библ. 13
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Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Сомова Л.А., Печуркин Н.С., Сарангова А.Б., Письмам Т.И., Полонский В.И.
Заглавие : Динамика системы "Растения - ризосферные микроогранизмы - искуственная почва" при повышенном содержании диоксида углерода в атмосфере : научное издание
Место публикации : 2-й Съезд. биофизиков России, Москва, 23-27 авг., 1999. - М., 1999. - Т.3. - С. 914. - ISBN 5-201-14417-9
ГРНТИ : 34.27.23
Предметные рубрики: ЭКОСИСТЕМЫ
ДИОКСИД УГЛЕРОДА
УВЕЛИЧЕНИЕ СОДЕРЖАНИЯ
ВЛИЯНИЕ
ДИНАМИКА
Аннотация: Изучена реакция простой наземной экосистемы "пшеница-ризосферные микроорганизмы-искусственная почва" на повышение содержания диоксида углерода в атмосфере до 0,06%. В этих условиях у растений, инокулированных ризосферными бактериями (Pseudomonas), скорость фотосинтеза повышается на 30% по сравнению с растениями, семена которых были не инокулированы бактериями. В системе с нормальным содержанием CO[2] в атмосфере (0,03%) скорость фотосинтеза у растений, инокулированных бактериями, увеличивается только на 10%. В экспериментах оценен вклад микроорганизмов в развитие системы. Растения с микроорганизмами в системе вовлекают в круговорот на 40% углерода больше, чем растения без микроорганизмов. При этом более 30% углерода, ассимилированного в системе, через корневые эксудаты идет на рост и дыхание микроорганизмов. Россия, Ин-т биофизики СО РАН, 660036 Красноярск, Академгородок
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Печуркин Н.С., Сомова Л.А., Полонский В.И., Письман Т.И., Сарангова А.Б., Полонская Д.Е., Садовская Г.М.
Заглавие : Влияние ризосферных бактерий Pseudomonas на рост молодых растений пшеницы в условиях полного минерального питания и при дефиците азота : научное издание
Место публикации : Микробиология. - 1997. - Т. 66, N 4. - С. 553-557. - ISSN 0026-3656
ГРНТИ : 68.03.07
Предметные рубрики: PSEUDOMONAS FLUORESCENS (BACT.)
PSEUDOMONAS PUTIDA (BACT.)
ВЛИЯНИЕ
РОСТ ПШЕНИЦЫ
РИЗОСФЕРНЫЕ МИКРООРГАНИЗМЫ
Аннотация: Изучено влияние двух видов ризосферных микроорганизмов Pseudomonas fluorescens и Pseudomonas putida на рост молодых растений пшеницы в условиях полного минерального питания и при дефиците азота в среде. Показано, что на полной минеральной среде растения, семена к-рых были инокулированы бактериями рода Pseudomonas (опытные растения), имели ростовые преимущества по сравнению с растениями, не инокулированными этими бактериями (контрольные растения). У опытных растений возрастала интенсивность транспирации, в биомассе растений было более высокое содержание органического азота. При дефиците азота в среде не отмечено изменений ростовых характеристик растений, инокулированных бактериями и без них. Не обнаружено конкуренции микроорганизмов с растениями за источники азота, так как лимитирующим фактором развития ризосферных микробов являлась скорость поступления органических веществ с корневыми эксудатами. Показано дифференцированное влияние ризосферных микроорганизмов на рост растений в зависимости от факторов среды
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Вид документа : Статья из сборника (выпуск монографической серии)
Шифр издания :
Автор(ы) : Gros J.-B., Lasseur C., Tikhomirov A.A., Manukovsky N.S., Ushakova S.A., Zolotukhin I.G., Tirranen L.S., Borodina E.V., Kovalev V.S.
Заглавие : Wheat growth on neutral and soil-like substrates: Carbon dioxide exchange and microflora
Место публикации : Acta Horticulturae. - 2004. - Vol. 644. - С. 243-248
Ключевые слова (''Своб.индексиров.''): carbon dioxide--closed ecological system
Аннотация: The soil-like substrate (SLS), which is a potential candidate for employment in closed ecological systems, has been tested. CO2 exchange, composition and numbers of soil microflora have been examined in .wheat-SLS. system. The results produced have been compared to analogous characteristics of .wheat-neutral substrate. system. A hydroponic method was used under wheat growing on the neutral substrate (expanded clay aggregate). Plants for both studies were grown in closed environment from seed to physiological maturity. In the .wheat-SLS. system, the net photosynthetic rate of canopy was positive in the course of 6.55 days after planting. The net photosynthetic rate of canopy in .wheat-neutral substrate. system was positive in the entire course of vegetation. According to calculations in the course of vegetation, photosynthesis has withdrawn 3.28 kg m-2 CO2 from the .wheat-SLS. system and 3.40 kg m-2 CO2 from the .wheat-neutral substrate. system. On the SLS dominant among bacteria were the spore-forming bacteria from Bacillus genus, among fungi . from Trichoderma genus. In the hydroponic cultivation on neutral substrate dominant were bacteria from Pseudomonas genus, most commonly found fungi were species from Fusarium and Botrytis genera.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prudnikova S., Streltsova N., Volova T.
Заглавие : The effect of the pesticide delivery method on the microbial community of field soil
Место публикации : Environ. Sci. Pollut. Res.: Springer Science and Business Media Deutschland GmbH, 2020. - Article in press. - ISSN 09441344 (ISSN), DOI 10.1007/s11356-020-11228-7
Аннотация: The study deals with the effects of herbicides (metribuzin, tribenuron-methyl, fenoxaprop-P-ethyl) and fungicides (tebuconazole, epoxiconazole, azoxystrobin) applied to soil as free pesticides or as slow release formulations embedded in a biodegradable composite matrix on the structure of the soil microbial community. The matrix consisted of a natural biopolymer poly-3-hydroxybutyrate [P(3HB)] and a filler—one of the natural materials (peat, clay, and wood flour). The soil microbial community was characterized, including the major eco-trophic groups of bacteria, dominant taxa of bacteria and fungi, and primary P(3HB)-degrading microorganisms, such as Pseudomonas, Bacillus, Pseudarthrobacter, Streptomyces, Penicillium, and Talaromyces. The addition of free pesticides adversely affected the abundance of soil microorganisms; the decrease varied from 1.4 to 56.0 times for different types of pesticides. The slow release pesticide formulations, in contrast to the free pesticides, exerted a much weaker effect on soil microorganisms, no significant inhibition in the abundance of saprotrophic bacteria was observed, partly due to the positive effects of the composite matrix (polymer/natural material), which was a supplementary substrate for microorganisms. The slow release fungicide formulations, like the free fungicides, reduced the total abundance of fungi and inhibited the development of the phytopathogens Fusarium and Alternaria. Thus, slow release formulations of pesticides preserve the bioremediation potential of soil microorganisms, which are the main factor of removing xenobiotics from the biosphere. © 2020, Springer-Verlag GmbH Germany, part of Springer Nature.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gros J.B., Lasseur Ch., Tikhomirov A.A., Manukovsky N.S., Kovalev V.S., Ushakova S.A., Zolotukhin I.G., Tirranen L.S., Karnachuk R.A., Dorofeev V.Yu.
Заглавие : Testing soil-like substrate for growing plants in bioregenerative life support systems
Место публикации : Advances in Space Research. - 2005. - Vol. 36, Is. 7. - С. 1312-1318. - ISSN 02731177 (ISSN) , DOI 10.1016/j.asr.2005.05.079
Ключевые слова (''Своб.индексиров.''): life support system--matter recycling--plants--soil-like substrate--biodiversity--correlation methods--growth kinetics--hormones--plants (botany)--recycling--soils--bioregeneration--life support system--matter recycling--soil-like substrate--space research
Аннотация: We studied soil-like substrate (SLS) as a potential candidate for plant cultivation in bioregenerative life support systems (BLSS). The SLS was obtained by successive conversion of wheat straw by oyster mushrooms and worms. Mature SLS contained 9.5% humic acids and 4.9% fulvic acids. First, it was shown that wheat, bean and cucumber yields as well as radish yields when cultivated on mature SLS were comparable to yields obtained on a neutral substrate (expanded clay aggregate) under hydroponics. Second, the possibility of increasing wheat and radish yields on the SLS was assessed at three levels of light intensity: 690, 920 and 1150 ?mol m-2 s-1 of photosynthetically active radiation (PAR). The highest wheat yield was obtained at 920 ?mol m-2 s-1, while radish yield increased steadily with increasing light intensity. Third, long-term SLS fertility was tested in a BLSS model with mineral and organic matter recycling. Eight cycles of wheat and 13 cycles of radish cultivation were carried out on the SLS in the experimental system. Correlation coefficients between SLS nitrogen content and total wheat biomass and grain yield were 0.92 and 0.97, respectively, and correlation coefficients between nitrogen content and total radish biomass and edible root yield were 0.88 and 0.87, respectively. Changes in hormone content (auxins, gibberellins, cytokinins and abscisic acid) in the SLS during matter recycling did not reduce plant productivity. Quantitative and species compositions of the SLS and irrigation water microflora were also investigated. Microbial community analysis of the SLS showed bacteria from Bacillus, Pseudomonas, Proteus, Nocardia, Mycobacterium, Arthrobacter and Enterobacter genera, and fungi from Trichoderma, Penicillium, Fusarium, Aspergillus, Mucor, Botrytis, and Cladosporium genera. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Somova L.A., Pechurkin N.S., Sarangova A.B., Pisman T.I., Polonsky V.I., Sadovskay G.M.
Заглавие : Small artificial ecosystems: response to variation of environmental factors (CO2 enrichment).
Место публикации : Life support & biosphere science : international journal of earth space. - 1999. - Vol. 6, Is. 3. - С. 215-220. - ISSN 10699422 (ISSN)
Ключевые слова (''Своб.индексиров.''): carbon--carbon dioxide--article--biomass--comparative study--drug effect--ecosystem--growth, development and aging--metabolism--microbiology--microclimate--photosynthesis--plant seed--pseudomonas--wheat--biomass--carbon--carbon dioxide--ecosystem--environment, controlled--photosynthesis--pseudomonas--seeds--soil microbiology--triticum
Аннотация: Response of "wheat plants--rhizospheric microorganisms--artificial soil"--a simple terrestrial ecosystem--to carbon dioxide increased in its atmosphere to 0.06% has been studied. It has been experimentally demonstrated that a simple ecosystem develops and functions different from its individual elements (components), in this case "plants-artificial soil" without microorganisms. With mineral nutrition unlimited and CO2 enrichment the system is capable of binding (involving into turnover) 40% more carbon than the system without microorganisms. With material balance as the basis, this article evaluates the contribution of a system's elements into its development, namely, the contribution of the photosynthesizing component and the contribution of microorganisms.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Lee J.
Заглавие : Purification and characterization of flavoproteins and cytochromes from the yellow bioluminescence marine bacterium Vibrio fischeri strain Y1
Место публикации : European Journal of Biochemistry. - 1997. - Vol. 245, Is. 3. - С. 790-796. - ISSN 00142956 (ISSN)
Ключевые слова (''Своб.индексиров.''): anisotropy--lumazine protein--photobacterium--thioredoxin reductase--time-resolved fluorescence--cytochrome--flavoprotein--article--bioluminescence--nonhuman--priority journal--protein analysis--protein purification--sea--vibrio--amino acid sequence--bacterial proteins--cytochromes--flavoproteins--molecular sequence data--sequence alignment--vibrio--azotobacter--bacteria (microorganisms)--escherichia coli--haemophilus--haemophilus influenza--murinae--negibacteria--photobacterium--photobacterium leiognathi--pseudomonas--uncultured marine bacterium--vibrio fischeri
Аннотация: Several flavoproteins and cytochromes that occur as major components in extracts of the yellow bioluminescence Y1 strain of the murine bacterium Vibrio fischeri have been purified and characterized with respect to their mass (SDS/PAGE) and matrix-assisted laser-desorption/ionization MS), chromatographic properties, N-terminal sequence, and spectroscopy (absorption, fluorescence emission and anisotropy decay). The investigated proteins were as follows: yellow fluorescence protein (YFP) with bound riboflavin, FMN or 6,7-dimethyl-8-ribityllumazine; a blue fluorescence protein (BFP) with bound 6,7-dimethyl-8-ribityllumazine, riboflavin, or 6- methyl-7-oxo-ribityllumazine; thioredoxin reductase with FAD as ligand; and two c-type diheme cytochromes, c551 and c554. We present evidence that the riboflavin-bound YFP has an N-terminal sequence corresponding to that published for the dimeric YFP. We show that an equilibrium replacement of the riboflavin can be made with excess lumazine derivative and that lumazine- bound YFP has different bioluminescence properties to those of the lumazine protein from Photobacterium leiognathi. BFP is a different protein again, and in the bacterial lysate it occurs in multiple forms, ligated to either riboflavin, lumazine, or t he 7-oxolumazine derivative. The N-terminal sequence for BFP-shows similarities to those of the YFP proteins and to lumazine protein and riboflavin synthase from Photobacterium. BFP in any form has no bioluminescence or riboflavin-synthase activity. A 70-kDa fluorescent flavoprotein with FAD as ligand has an N-terminal sequence highly similar to those of thioredoxin reductases from Haemophilus influenza and Escherichia coli. Cytochrome contaminations in previous preparations of YFP have been removed and an identified as the two c-type cytochromes c551 and c554. Both inhibit the NADH-induced bioluminescence in the reductase/luciferase system with the luciferase from P. leiognathi and V. fischeri. The N-terminal amino acid sequence of the cytochrome (c551) corresponds to a diheme cytochrome c4. The spectral properties of c554 are similar to those of other c5 cytochromes, and both c554 and c551 have absorption spectra similar to those of the respective cytochromes from the gram-negative bacteria Pseudomonas and Azotobacter.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Khizhnyak S.V., Tausheva I.V., Berezikova A.A., Nesterenko E.V., Rogozin D.Y.
Заглавие : Psychrophilic and psychrotolerant heterotrophic microorganisms of Middle Siberian karst cavities
Колич.характеристики :5 с
Место публикации : Russ. J. Ecol.: MAIK NAUKA/INTERPERIODICA, 2003. - Vol. 34, Is. 4. - P231-235. - ISSN 1067-4136, DOI 10.1023/A:1024537513439
Примечания : Cited References: 12
Ключевые слова (''Своб.индексиров.''): psychrophilic and psychrotolerant microorganisms--karst cavities--caves--heterotrophic microorganisms
Аннотация: The natural microflora of Middle Siberian karst cavities, which comprises psychrotolerant bacteria and fungi capable of growing at 3-15 and 3...+28degreesC, respectively, has been studied. Bacteria are ubiquitous in caves, their count varying from 10(3) to 10(7) cells/g ground. The bacteria have been identified as Pseudomonas, Arthrobacter, Bacillus, and coryneform bacteria. Fungi have been found in places exposed to increased anthropogenic impact, their count being as large as 10(6) to 10(7) cells/g ground. Mucor Penicillium, and Chrysosporium were dominant fungal genera.
WOS
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Khizhnyak S.V., Tausheva I.V., Berezikova A.A., Nesterenko E.V., Rogozin D.Yu.
Заглавие : Psychrophilic and psychrotolerant heterotrophic microorganisms of Middle Siberian karst cavities
Место публикации : Russian Journal of Ecology. - 2003. - Vol. 34, Is. 4. - С. 231-235. - ISSN 10674136 (ISSN) , DOI 10.1023/A:1024537513439
Ключевые слова (''Своб.индексиров.''): caves--heterotrophic microorganisms--karst cavities--psychorophilic and psychrotolerant microorganisms--bacterium--cavernicolous species--fungus--heterotrophy--karst--low temperature--russian federation--arthrobacter--bacteria (microorganisms)--chrysosporium--corynebacteriaceae--fungi--mucor--penicillium--pseudomonas
Аннотация: The natural microflora of Middle Siberian karst cavities, which comprises psychrotolerant bacteria and fungi capable of growing at 3-15 and 3...+28В°C, respectively, has been studied. Bacteria are ubiquitous in caves, their count varying from 103 to 107 cells/g ground. The bacteria have been identified as Pseudomonas, Arthrobacter, Bacillus, and coryneform bacteria. Fungi have been found in places exposed to increased anthropogenic impact, their count being as large as 106 to 107 cells/g ground. Mucor, Penicillium, and Chrysosporium were dominant fungal genera.
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12.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Pisman T.I., Galayda Y.V., Loginova N.S.
Заглавие : Population dynamics of an algal-bacterial cenosis in closed ecological system
Колич.характеристики :5 с
Место публикации : SPACE LIFE SCIENCES: CLOSED ECOLOGICAL SYSTEMS: EARTH AND SPACE APPLICATIONS. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON-ELSEVIER SCIENCE LTD, 2005. - Vol. 35: Workshop on Closed Ecological Systems (JUL, 2004, Paris, FRANCE), Is. 9. - С. 1579-1583. - ISBN 0273-1177, DOI 10.1016/j.asr.2005.03.073
Примечания : Cited References: 14
Ключевые слова (''Своб.индексиров.''): microalgae-bacteria relationships--photosynthates--detritus--chlorella--biotic cycle
Аннотация: The paper deals with microalgae-bacteria interrelationships in the "autotroph-heterotroph" aquatic biotic cycle. Explanations of why and how algal-bacterial ecosystems are formed still remain controversial. The paper presents results of experimental and theoretical investigations of the functioning of the algal-bacterial cenosis (the microalga Chlorella vulgaris and concomitant microflora). The Chlorella microbial community is dominated by representatives of the genus Pseudomonas. Experiments with non-sterile batch cultures of Chlorella on Tamiya medium showed that the biomass of microorganisms increases simultaneously with the increase in microalgal biomass. The microflora of Chlorella can grow on organic substances released by photosynthesizing Chlorella. Microorganisms can also use dying Chlorella cells, i.e. form a "producer-reducer" biocycle. To get a better insight into the cenosis-forming role of microalgae, a mathematical model of the "autotroph-heterotroph" aquatic biotic cycle has been constructed, taking into account the utilization of Chlorella photosynthates and dead cells by microorganisms and the contribution of the components to the nitrogen cycle. A theoretical study showed that the biomass of concomitant bacteria grown on glucose and detritus is larger than the biomass of bacteria utilizing only microalgal photosynthates, which agrees well with the experimental data. (c) 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Somova L.A., Pechurkin N.S., Polonsky V.I., Pisman T.I., Sarangova A.B., Andre M., Sadovskaya G.M.
Заглавие : Plants-rhizospheric organisms interaction in a manmade system with and without biogenous element limitation
Место публикации : Advances in Space Research. - 1997. - Vol. 20, Is. 10. - С. 1939-1943. - ISSN 02731177 (ISSN)
Ключевые слова (''Своб.индексиров.''): nitrogen--article--bacterial count--biomass--comparative study--culture medium--growth, development and aging--metabolism--microbiology--plant leaf--plant root--plant seed--pseudomonas fluorescens--pseudomonas putida--wheat--biomass--colony count, microbial--culture media--nitrogen--plant leaves--plant roots--pseudomonas fluorescens--pseudomonas putida--seeds--triticum
Аннотация: The effect has been studied of inoculation of seeds of wheat with two species of rhizospheric microorganisms, -Pseudomonas fluorescens and Pseudomonas putida - on young plant growth with complete and with nitrogen deficit mineral nutrition. With complete mineral medium, plants grown from seeds inoculated with bacteria of Pseudomonas genus (experiment plants) have been found to have better growth over plants not inoculated with these bacteria (control plants). The experiment plants had increased transpiration and their biomass had higher organic nitrogen content. With nitrogen deficit medium, the plants inoculated with bacteria and those without them, have not revealed changes in growth. Neither case demonstrated competition of microorganisms with plants for nitrogen sources. В© 1997 COSPAR. Published by Elsevier Science Ltd.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sarangova A.B., Somova L.A., Pechurkin N.S.
Заглавие : On monitoring the bacterial component as an indicator of the state of small man-made ecosystems
Место публикации : Advances in Space Research. - 2001. - Vol. 27, Is. 9. - С. 1605-1609. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(01)00256-3
Ключевые слова (''Своб.индексиров.''): bacteria--ecosystems--substrates--intracellular substrate concentration--space research--catalase--oxidoreductase--artificial ecosystem--article--bacterial phenomena and functions--biomass--culture medium--ecosystem--enzymology--growth, development and aging--metabolism--microbiology--oxygen consumption--pseudomonas--bacterial physiology--biomass--catalase--culture media--ecosystem--oxidoreductases--oxygen consumption--pseudomonas--water microbiology
Аннотация: High reproduction rates make the bacterial component of ecosystems a good indicator of the state of the system on the whole. This determines the necessity to develop rapid monitoring of the functional state of the bacterial component of small ecosystems. Information about substrate concentration in the population is indicative of the state of the bacterial culture. Conventional methods of monitoring the concentration of integral substrate in the system take time much longer than the changes in the ecosystem. The paper presents theoretical foundations for the logical sequence "catalase activity - intracellular substrate concentration - estimate of substrate consumed by bacteria" for experimental verification and as a consequence of development of the integral method of monitoring the bacterial population on the basis of determining bacterial catalase activity. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Belyaeva O.G., Gitelzon I.I., Kalacheva G.S., Lukovenko S.G., Plotnikov V.F.
Заглавие : Obtain and research of microbial heteropolymeric polyhydroxyalkanoates
Место публикации : Dokl. Akad. Nauk: MEZHDUNARODNAYA KNIGA, 1996. - Vol. 347, Is. 2. - С. 256-258. - 3. - ISSN 0869-5652
Примечания : Cited References: 12
Предметные рубрики: PSEUDOMONAS
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova, Tatiana G., Prudnikova, Svetlana V., Vinogradova, Olga N., Syrvacheva, Darya A., Shishatskaya, Ekaterina I.
Заглавие : Microbial Degradation of Polyhydroxyalkanoates with Different Chemical Compositions and Their Biodegradability
Колич.характеристики :15 с
Коллективы : Russian Science Foundation [14-26-00039]
Место публикации : Microb. Ecol.: SPRINGER, 2017. - Vol. 73, Is. 2. - С. 353-367. - ISSN 0095-3628, DOI 10.1007/s00248-016-0852-3. - ISSN 1432-184X(eISSN)
Примечания : Cited References:65. - This study was supported by the Russian Science Foundation (Grant No. 14-26-00039).
Предметные рубрики: TROPICAL COASTAL WATERS
PHA-DEGRADING BACTERIA
ALCALIGENES-EUTROPHUS
Ключевые слова (''Своб.индексиров.''): degradable polyhydroxyalkanoates--pha--soil--pha-degrading--microorganisms
Аннотация: The study addresses degradation of polyhydroxyalkanoates (PHA) with different chemical compositions-the polymer of 3-hydroxybutyric acid [P(3HB)] and copolymers of P(3HB) with 3-hydroxyvalerate [P(3HB/3HV)], 4-hydroxybutyrate [P(3HB/4HB)], and 3-hydroxyhexanoate [P(3HB/3HHx)] (10-12 mol%)-in the agro-transformed field soil of the temperate zone. Based on their degradation rates at 21 and 28 A degrees C, polymers can be ranked as follows: P(3HB/4HB) P(3HB/3HHx) P(3HB/3HV) P(3HB). The microbial community on the surface of the polymers differs from the microbial community of the soil with PHA specimens in the composition and percentages of species. Thirty-five isolates of bacteria of 16 genera were identified as PHA degraders by the clear zone technique, and each of the PHA had both specific and common degraders. P(3HB) was degraded by bacteria of the genera Mitsuaria, Chitinophaga, and Acidovorax, which were not among the degraders of the three other PHA types. Roseateles depolymerans, Streptomyces gardneri, and Cupriavidus sp. were specific degraders of P(3HB/4HB). Roseomonas massiliae and Delftia acidovorans degraded P(3HB/3HV), and Pseudoxanthomonas sp., Pseudomonas fluorescens, Ensifer adhaerens, and Bacillus pumilus were specific P(3HB/3HHx) degraders. All four PHA types were degraded by Streptomyces.
WOS,
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prudnikova S. V., Evgrafova S. Y., Volova T. G.
Заглавие : Metabolic activity of cryogenic soils in the subarctic zone of Siberia towards “green” bioplastics
Место публикации : Chemosphere: Elsevier Ltd, 2021. - Vol. 263. - Ст.128180. - ISSN 00456535 (ISSN), DOI 10.1016/j.chemosphere.2020.128180
Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic – poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9–11 °C, the total biomass of microorganisms at a 10-20-cm depth was 226–234 mg g?1 soil and CO2 production was 20–46 mg g?1 day?1. The total abundance of microscopic fungi varied between (7.4 ± 2.3) ? 103 and (18.3 ± 2.2) ? 103 CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 ± 0.1) ? 106 CFU g?1 soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique – plating of isolates on the mineral agar with polymer as sole carbon source – was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)-degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. © 2020 Elsevier Ltd
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prudnikova, Svetlana, V, Evgrafova, Svetlana Yu, Volova, Tatiana G.
Заглавие : Metabolic activity of cryogenic soils in the subarctic zone of Siberia towards "green" bioplastics
Колич.характеристики :12 с
Коллективы : Project "Agropreparations of the new generation: a strategy of construction and realization" [074-02-2018-328]; Government of the Russian Federation [220]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Место публикации : Chemosphere: PERGAMON-ELSEVIER SCIENCE LTD, 2021. - Vol. 263. - Ст.128180. - ISSN 0045-6535, DOI 10.1016/j.chemosphere.2020.128180. - ISSN 1879-1298(eISSN)
Примечания : Cited References:101. - This study (polymer synthesis and investigation) was financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 074-02-2018-328) in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning", and by the State assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (investigation of polymer degradation in soils of Evenkia).
Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic - poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9-11 degrees C, the total biomass of microorganisms at a 10-20-cm depth was 226-234 mg g(-1) soil and CO2 production was 20-46 mg g(-1)W day(-1). The total abundance of microscopic fungi varied between (7.4 +/- 2.3) x 10(3) and (18.3 +/- 2.2) x 10(3) CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 +/- 0.1) x 10(6) CFU g(-1) soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique e plating of isolates on the mineral agar with polymer as sole carbon source e was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. (C) 2020 Elsevier Ltd. All rights reserved.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pis'man T.I., Somova L.A., Pechurkin N.S.
Заглавие : Mathematical model of the interaction of components in a plant-rhizospheric microorganisms system at the higher level of carbon dioxide in atmosphere
Место публикации : Biofizika. - 2002. - Vol. 47, Is. 5. - С. 920-925. - ISSN 00063029 (ISSN)
Ключевые слова (''Своб.индексиров.''): carbon dioxide--algorithm--article--biological model--biomass--ecosystem--microbiology--physiology--plant seed--pseudomonas putida--wheat--algorithms--biomass--carbon dioxide--ecosystem--models, biological--pseudomonas putida--seeds--triticum
Аннотация: A mathematical model describing the interaction of plants and rhizospheric microorganisms on complete mineral medium at a higher CO2 level in the atmosphere was constructed. The positive effect of CO2-enrichment on the system plant--rhizospheric microorganisms was shown. The effect of rhizospheric microorganisms on plant growth at normal and high level of carbon dioxide was demonstrated. It was shown that the biomass of plant in the system is smaller than the biomass of plant growing without microorganisms. It was experimentally demonstrated that a simple ecosystem wheat--Pseudomonas putida--artificial soil develops and functions differently than its individual constituents in the case of a wheat-artificial soil system. With unlimited nutrition and a higher CO2 level (0.06%), plants with roots inoculated with microorganisms have a smaller biomass than plants that were not inoculated with microorganisms.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pechurkin N.S., Somova L.A., Polonskii V.I., Pisman T.I., Sarangova A.B., Polonskaya D.E., Sadovskaya G.M.
Заглавие : Influence of rhizosphere bacteria of the genus Pseudomonas on the growth of wheat seedlings under conditions of complete mineral supply and nitrogen deficiency
Колич.характеристики :5 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1997. - Vol. 66, Is. 4. - С. 461-465. - ISSN 0026-2617
Примечания : Cited References: 9
Ключевые слова (''Своб.индексиров.''): pseudomonas--wheat--nitrogen--growth--biomass--transpiration
Аннотация: The influence of two rhizosphere bacteria-Pseudomonas fluorescens and Pseudomonas putida-on the growth of wheat seedlings was studied under conditions of complete mineral supply and nitrogen deficiency in the medium. On complete mineral medium, the plants that developed from the seeds inoculated with the pseudomonads had some advantages for growth over those developed from the non-inoculated seeds. Inoculated plants showed an increased transpiration and a higher content of organic nitrogen in their biomass. Under conditions of nitrogen deficiency in the medium, no differences were found between inoculated and noninoculated plants. No competition for the nitrogen sources was revealed between the bacteria and plants; the development of the rhizosphere bacteria was limited by the rate of organic compound excretion by plant roots. The stimulatory effect produced on plant growth by the rhizosphere bacteria was shown to vary depending on the environmental factors.
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