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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Lesniak D.V., Popova L.I.
Заглавие : Conflict: induction-inhibition of transgene bacteria luminescence in studying expression of lux-genes
Место публикации : Biofizika. - 2002. - Vol. 47, Is. 6. - P1059-1063. - ISSN 00063029 (ISSN)
Ключевые слова (''Своб.индексиров.''): naphthalene derivative--salicylic acid derivative--article--bacterial gene--chemistry--chemoluminescence--culture medium--escherichia coli--gene expression regulation--genetics--metabolism--pseudomonas fluorescens--transgene--chemiluminescent measurements--culture media--escherichia coli--gene expression regulation, bacterial--genes, bacterial--naphthalenes--pseudomonas fluorescens--salicylates--transgenes
Аннотация: The relationship between the induction of the luminescent operon of lux-genes fused with the naphthalene and salicylate degradation genes and the inhibition of light emission caused by these compounds was studied. The quantitative correlations between these processes manifest themselves in the fact that light intensity linearly increased in a narrow concentration range of the inductor and then decreased due to the inhibition of the luminescence reaction itself, which is not related to the regulation of expression of lux-genes.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Lee J.
Заглавие : Purification and characterization of flavoproteins and cytochromes from the yellow bioluminescence marine bacterium Vibrio fischeri strain Y1
Место публикации : European Journal of Biochemistry. - 1997. - Vol. 245, Is. 3. - С. 790-796. - ISSN 00142956 (ISSN)
Ключевые слова (''Своб.индексиров.''): anisotropy--lumazine protein--photobacterium--thioredoxin reductase--time-resolved fluorescence--cytochrome--flavoprotein--article--bioluminescence--nonhuman--priority journal--protein analysis--protein purification--sea--vibrio--amino acid sequence--bacterial proteins--cytochromes--flavoproteins--molecular sequence data--sequence alignment--vibrio--azotobacter--bacteria (microorganisms)--escherichia coli--haemophilus--haemophilus influenza--murinae--negibacteria--photobacterium--photobacterium leiognathi--pseudomonas--uncultured marine bacterium--vibrio fischeri
Аннотация: Several flavoproteins and cytochromes that occur as major components in extracts of the yellow bioluminescence Y1 strain of the murine bacterium Vibrio fischeri have been purified and characterized with respect to their mass (SDS/PAGE) and matrix-assisted laser-desorption/ionization MS), chromatographic properties, N-terminal sequence, and spectroscopy (absorption, fluorescence emission and anisotropy decay). The investigated proteins were as follows: yellow fluorescence protein (YFP) with bound riboflavin, FMN or 6,7-dimethyl-8-ribityllumazine; a blue fluorescence protein (BFP) with bound 6,7-dimethyl-8-ribityllumazine, riboflavin, or 6- methyl-7-oxo-ribityllumazine; thioredoxin reductase with FAD as ligand; and two c-type diheme cytochromes, c551 and c554. We present evidence that the riboflavin-bound YFP has an N-terminal sequence corresponding to that published for the dimeric YFP. We show that an equilibrium replacement of the riboflavin can be made with excess lumazine derivative and that lumazine- bound YFP has different bioluminescence properties to those of the lumazine protein from Photobacterium leiognathi. BFP is a different protein again, and in the bacterial lysate it occurs in multiple forms, ligated to either riboflavin, lumazine, or t he 7-oxolumazine derivative. The N-terminal sequence for BFP-shows similarities to those of the YFP proteins and to lumazine protein and riboflavin synthase from Photobacterium. BFP in any form has no bioluminescence or riboflavin-synthase activity. A 70-kDa fluorescent flavoprotein with FAD as ligand has an N-terminal sequence highly similar to those of thioredoxin reductases from Haemophilus influenza and Escherichia coli. Cytochrome contaminations in previous preparations of YFP have been removed and an identified as the two c-type cytochromes c551 and c554. Both inhibit the NADH-induced bioluminescence in the reductase/luciferase system with the luciferase from P. leiognathi and V. fischeri. The N-terminal amino acid sequence of the cytochrome (c551) corresponds to a diheme cytochrome c4. The spectral properties of c554 are similar to those of other c5 cytochromes, and both c554 and c551 have absorption spectra similar to those of the respective cytochromes from the gram-negative bacteria Pseudomonas and Azotobacter.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Altukhova O.V.
Заглавие : Autotrophic synthesis of polyhydroxyalkanoates by the bacteria Ralstonia eutropha in the presence of carbon monoxide
Колич.характеристики :4 с
Место публикации : Appl. Microbiol. Biotechnol.: SPRINGER-VERLAG, 2002. - Vol. 58, Is. 5. - P675-678. - ISSN 0175-7598, DOI 10.1007/s00253-002-0941-8
Примечания : Cited References: 35
Предметные рубрики: PSEUDOMONAS-OLEOVORANS
ALCALIGENES-EUTROPHUS
COAL
CULTIVATION
METABOLISM
SUBSTRATE
CULTURE
CO2
H-2
O-2
Аннотация: It has been found that the carbon monoxide (CO)-resistant strain of the hydrogen bacteria Ralstonia eutropha B5786 is able to synthesise polyhydroxyalkanoates (PHAs) in the presence of CO under autotrophic conditions. This strain, grown on model gas mixtures containing 5-25% CO v/v), accumulates up to 70-75% (of absolutely dry matter) PHA, without significant variation in the yield coefficient on hydrogen. No suppression of the activities of the key enzymes of PHA synthesis (beta-ketothiolase, acetoacetyl-CoA-reductase, butyrate dehydrogenase and poly-3-hydroxybutyrate synthase) was recorded. The PHA synthesised is a copolymer containing mostly beta-hydroxybutyrate (more than 99 mol%) with trace amounts of beta-hydroxyvalerate. The investigated properties of the polymer (molecular weight, crystallinity, temperature characteristics) do not differ from those of the polymer synthesised on electrolytic hydrogen.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pisman T.I., Somova L.A., Pechurkin N.S.
Заглавие : A mathematical model of the interaction of the components in a system plant-rhizospheric microorganisms at a higher CO2 level in the atmosphere
Колич.характеристики :6 с
Место публикации : Biofizika: MEZHDUNARODNAYA KNIGA, 2002. - Vol. 47, Is. 5. - P920-925. - ISSN 0006-3029
Примечания : Cited References: 13
Предметные рубрики: CARBON-DIOXIDE
ENRICHMENT
ECOSYSTEMS
Ключевые слова (''Своб.индексиров.''): plant--rhizospheric microorganisms--ecosystem--co2-enrichnient
Аннотация: A mathematical model describing the interaction of plants and rhizospheric microorganisms on complete mineral medium at a higher CO2 level in the atmosphere was constructed. The positive effect of CO2-enrichment on the system plant - rhizospheric micro organisms was shown. The effect of rhizospheric microorganisms on plant growth at normal and high level of carbon dioxide was demonstrated. It was shown that the biomass of plant in the system is smaller than the biomass of plant growing without microorganisms. It was experimentally demonstrated that a simple ecosystem wheat - Pseudomonas putida - artificial soil develops and functions differently than its individual constituents in the case of a wheat-artificial soil system. With unlimited nutrition and a higher CO2 level (0.06%), plants with roots inoculated with micro organisms have a smaller biomass than plants that were not inoculated with micro organisms.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pechurkin N.S., Somova L.A., Polonskii V.I., Pisman T.I., Sarangova A.B., Polonskaya D.E., Sadovskaya G.M.
Заглавие : Influence of rhizosphere bacteria of the genus Pseudomonas on the growth of wheat seedlings under conditions of complete mineral supply and nitrogen deficiency
Колич.характеристики :5 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1997. - Vol. 66, Is. 4. - С. 461-465. - ISSN 0026-2617
Примечания : Cited References: 9
Ключевые слова (''Своб.индексиров.''): pseudomonas--wheat--nitrogen--growth--biomass--transpiration
Аннотация: The influence of two rhizosphere bacteria-Pseudomonas fluorescens and Pseudomonas putida-on the growth of wheat seedlings was studied under conditions of complete mineral supply and nitrogen deficiency in the medium. On complete mineral medium, the plants that developed from the seeds inoculated with the pseudomonads had some advantages for growth over those developed from the non-inoculated seeds. Inoculated plants showed an increased transpiration and a higher content of organic nitrogen in their biomass. Under conditions of nitrogen deficiency in the medium, no differences were found between inoculated and noninoculated plants. No competition for the nitrogen sources was revealed between the bacteria and plants; the development of the rhizosphere bacteria was limited by the rate of organic compound excretion by plant roots. The stimulatory effect produced on plant growth by the rhizosphere bacteria was shown to vary depending on the environmental factors.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A. N., Prudnikova S.V., Filipenko M.L., Khrapov E.A., Vasiliev A. D., Volova, Tatiana G.
Заглавие : Biodegradation of polyhydroxyalkanoates by soil microbial communities of different structures and detection of PHA degrading microorganisms
Коллективы : Government of the Russian Federation [11.G34.31.0013]; Presidium of the Siberian Branch of Russian Academy of Sciences [96]
Место публикации : Appl. Biochem. Microbiol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2012. - Vol. 48, Is. 1. - С. 28-36. - 9. - ISSN 0003-6838, DOI 10.1134/S0003683812010024
Примечания : Cited References: 39. - The work was supported by the project initiated by the Government of the Russian Federation for governmental support of scientific research conducted under the guidance of leading scientists at Russian institutions of higher learning (Agreement No. 11.G34.31.0013) and the Program of Integrated Research of the Presidium of the Siberian Branch of Russian Academy of Sciences (project no. 96).
Предметные рубрики: POLY-BETA-HYDROXYBUTYRATE
CHAIN-LENGTH POLYHYDROXYALKANOATES
DEGRADATION
FILMS
POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)
BACTERIA
ACID
Аннотация: Biodegradation of microbial linear polymers of hydroxyalkanoic acids (polyhydroxyalkanoates, PHAs) by soil microbial communities of different structures has been studied during two field seasons in different weather conditions. This process was shown to be influenced by the polymer chemical composition, temperature, humidity, and the microbial soil component. The PHA degradation was accompanied by a decrease in the polymer molecular weight and an increase in the degree of crystallinity, indicating the preferential destruction of the amorphous phase compared to the crystalline one. The quantity of the true PHA destructors developing at the surface of the polymer samples was lower than the quantity of accompanying bacteria. The dominant PHA degrading microorganisms under the test conditions were identified as bacteria of the genera Variovorax, Stenotrophomonas, Acinetobacter, Pseudomonas, Bacillus, and Xanthomonas and as micromycetes from Penicillium, Paecilomyces, Acremonium, Verticillium, and Zygosporium.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Belyaeva O.G., Gitelzon I.I., Kalacheva G.S., Lukovenko S.G., Plotnikov V.F.
Заглавие : Obtain and research of microbial heteropolymeric polyhydroxyalkanoates
Место публикации : Dokl. Akad. Nauk: MEZHDUNARODNAYA KNIGA, 1996. - Vol. 347, Is. 2. - С. 256-258. - 3. - ISSN 0869-5652
Примечания : Cited References: 12
Предметные рубрики: PSEUDOMONAS
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Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Pisman T.I., Galayda Y.V., Loginova N.S.
Заглавие : Population dynamics of an algal-bacterial cenosis in closed ecological system
Колич.характеристики :5 с
Место публикации : SPACE LIFE SCIENCES: CLOSED ECOLOGICAL SYSTEMS: EARTH AND SPACE APPLICATIONS. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON-ELSEVIER SCIENCE LTD, 2005. - Vol. 35: Workshop on Closed Ecological Systems (JUL, 2004, Paris, FRANCE), Is. 9. - С. 1579-1583. - ISBN 0273-1177, DOI 10.1016/j.asr.2005.03.073
Примечания : Cited References: 14
Ключевые слова (''Своб.индексиров.''): microalgae-bacteria relationships--photosynthates--detritus--chlorella--biotic cycle
Аннотация: The paper deals with microalgae-bacteria interrelationships in the "autotroph-heterotroph" aquatic biotic cycle. Explanations of why and how algal-bacterial ecosystems are formed still remain controversial. The paper presents results of experimental and theoretical investigations of the functioning of the algal-bacterial cenosis (the microalga Chlorella vulgaris and concomitant microflora). The Chlorella microbial community is dominated by representatives of the genus Pseudomonas. Experiments with non-sterile batch cultures of Chlorella on Tamiya medium showed that the biomass of microorganisms increases simultaneously with the increase in microalgal biomass. The microflora of Chlorella can grow on organic substances released by photosynthesizing Chlorella. Microorganisms can also use dying Chlorella cells, i.e. form a "producer-reducer" biocycle. To get a better insight into the cenosis-forming role of microalgae, a mathematical model of the "autotroph-heterotroph" aquatic biotic cycle has been constructed, taking into account the utilization of Chlorella photosynthates and dead cells by microorganisms and the contribution of the components to the nitrogen cycle. A theoretical study showed that the biomass of concomitant bacteria grown on glucose and detritus is larger than the biomass of bacteria utilizing only microalgal photosynthates, which agrees well with the experimental data. (c) 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondar V.S., Boersma M.G., Golovlev E.L., Vervoort J..., Van Berkel WJH, Finkelstein Z.I., Solyanikova I.P., Golovleva L.A., Rietjens IMCM
Заглавие : (19)F NMR study on the biodegradation of fluorophenols by various Rhodococcus species
Колич.характеристики :12 с
Место публикации : Biodegradation: SPRINGER, 1998. - Vol. 9: NMR in Environmental Sciences Symposium (OCT 24, 1997, EDE, NETHERLANDS), Is. 6. - С. 475-486. - ISSN 0923-9820, DOI 10.1023/A:1008391906885
Примечания : Cited References: 37
Предметные рубрики: NUCLEAR-MAGNETIC-RESONANCE
PSEUDOMONAS-PUTIDA MT-2
PHENOL HYDROXYLASE
BACTERIAL-DEGRADATION
3-OXOADIPATE PATHWAY
FLUOROBENZOIC ACID
CONVERSION
METABOLISM
CYCLOISOMERASES
2-CHLORO-CIS,CIS-MUCONATE
Ключевые слова (''Своб.индексиров.''): biodegradation--fluorophenols--(19)f nmr--oxidative defluorination--rhodococcus species
Аннотация: Of all NMR observable isotopes (19)F is the one perhaps most convenient for studies on biodegradation of environmental pollutants. The reasons underlying this potential of (19)F NMR are discussed and illustrated on the basis of a study on the biodegradation of fluorophenols by four Rhodococcus strains. The results indicate marked differences between the biodegradation pathways of fluorophenols among the various Rhodococcus species. This holds not only for the level and nature of the fluorinated biodegradation pathway intermediates that accumulate, but also for the regioselectivity of the initial hydroxylation step. Several of the Rhodococcus species contain a phenol hydroxylase that catalyses the oxidative defluorination of ortho-fluorinated di- and trifluorophenols. Furthermore, it is illustrated how the (19)F NMR technique can be used as a tool in the process of identification of an accumulated unknown metabolite, in this case most likely 5-fluoromaleylacetate. Altogether, the (19)F NMR technique proved valid to obtain detailed information on the microbial biodegradation pathways of fluorinated organics, but also to provide information on the specificity of enzymes generally considered unstable and, for this reason, not much studied so far.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Khizhnyak S.V., Tausheva I.V., Berezikova A.A., Nesterenko E.V., Rogozin D.Y.
Заглавие : Psychrophilic and psychrotolerant heterotrophic microorganisms of Middle Siberian karst cavities
Колич.характеристики :5 с
Место публикации : Russ. J. Ecol.: MAIK NAUKA/INTERPERIODICA, 2003. - Vol. 34, Is. 4. - P231-235. - ISSN 1067-4136, DOI 10.1023/A:1024537513439
Примечания : Cited References: 12
Ключевые слова (''Своб.индексиров.''): psychrophilic and psychrotolerant microorganisms--karst cavities--caves--heterotrophic microorganisms
Аннотация: The natural microflora of Middle Siberian karst cavities, which comprises psychrotolerant bacteria and fungi capable of growing at 3-15 and 3...+28degreesC, respectively, has been studied. Bacteria are ubiquitous in caves, their count varying from 10(3) to 10(7) cells/g ground. The bacteria have been identified as Pseudomonas, Arthrobacter, Bacillus, and coryneform bacteria. Fungi have been found in places exposed to increased anthropogenic impact, their count being as large as 10(6) to 10(7) cells/g ground. Mucor Penicillium, and Chrysosporium were dominant fungal genera.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prudnikova S., Streltsova N., Volova T.
Заглавие : The effect of the pesticide delivery method on the microbial community of field soil
Место публикации : Environ. Sci. Pollut. Res.: Springer Science and Business Media Deutschland GmbH, 2020. - Article in press. - ISSN 09441344 (ISSN), DOI 10.1007/s11356-020-11228-7
Аннотация: The study deals with the effects of herbicides (metribuzin, tribenuron-methyl, fenoxaprop-P-ethyl) and fungicides (tebuconazole, epoxiconazole, azoxystrobin) applied to soil as free pesticides or as slow release formulations embedded in a biodegradable composite matrix on the structure of the soil microbial community. The matrix consisted of a natural biopolymer poly-3-hydroxybutyrate [P(3HB)] and a filler—one of the natural materials (peat, clay, and wood flour). The soil microbial community was characterized, including the major eco-trophic groups of bacteria, dominant taxa of bacteria and fungi, and primary P(3HB)-degrading microorganisms, such as Pseudomonas, Bacillus, Pseudarthrobacter, Streptomyces, Penicillium, and Talaromyces. The addition of free pesticides adversely affected the abundance of soil microorganisms; the decrease varied from 1.4 to 56.0 times for different types of pesticides. The slow release pesticide formulations, in contrast to the free pesticides, exerted a much weaker effect on soil microorganisms, no significant inhibition in the abundance of saprotrophic bacteria was observed, partly due to the positive effects of the composite matrix (polymer/natural material), which was a supplementary substrate for microorganisms. The slow release fungicide formulations, like the free fungicides, reduced the total abundance of fungi and inhibited the development of the phytopathogens Fusarium and Alternaria. Thus, slow release formulations of pesticides preserve the bioremediation potential of soil microorganisms, which are the main factor of removing xenobiotics from the biosphere. © 2020, Springer-Verlag GmbH Germany, part of Springer Nature.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Efremov A. A., Zykova I. D., Korosteleva N. S.
Заглавие : Antimicrobial and antiradical activity of individual fractions of essential oil from seeds of heracleum dissectum ledeb. Of Siberian Region
Место публикации : Khimiya Rastitel'nogo Syr'ya: Altai State University, 2020. - Is. 2. - С. 79-85. - ISSN 10295151 (ISSN), DOI 10.14258/JCPRM.2020027029
Аннотация: By the method of exhaustive hydroponically obtained essential oil from beans of Heracleum dissectum Ledeb., growing in the Krasnoyarsk region. Separate fractions of oil were obtained: the first after 45 minutes from the beginning of distillation, the second – after 2 hours, the third-after 5 hours, the fourth fraction was collected after the end of hydro-distillation. The component composition of both whole essential oil and its separate fractions was studied. The main components are octyl acetate (60.0%), octyl-2-methylpropanoate (10.2%), n-hexyl-2-methylbutanoate (9.0%). The main amount of octyl acetate (64.7%) is concentrated in the first fraction of the oil. The antimicrobial activity of various fractions of essential oil of borscht dissected against strains of opportunistic microorganisms: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus 209p, MRSA, Proteus vulgaris. It was found that, depending on the duration of isolation, the antimicrobial activity of essential oil fractions in relation to Staphylococcus aureus 209p, MRSA and Pseudomonas aeruginosa decreases, and in relation to Escherichia coli, Klebsiella pneumoniae and Proteus vulgaris increases. The most pronounced inhibitory effect of the third and fourth fractions of essential oil against Klebsiella pneumonia. The antiradical activity of all studied samples of borscht essential oil dissected in reaction with stable free 2,2-diphenyl-1-picrylhydrazyl radical was established. The first fraction showed minimal antiradical activity (15.1%), the fourth – maximum (49.2%). © 2020 Altai State University. All rights reserved.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prudnikova, Svetlana, V, Evgrafova, Svetlana Yu, Volova, Tatiana G.
Заглавие : Metabolic activity of cryogenic soils in the subarctic zone of Siberia towards "green" bioplastics
Колич.характеристики :12 с
Коллективы : Project "Agropreparations of the new generation: a strategy of construction and realization" [074-02-2018-328]; Government of the Russian Federation [220]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Место публикации : Chemosphere: PERGAMON-ELSEVIER SCIENCE LTD, 2021. - Vol. 263. - Ст.128180. - ISSN 0045-6535, DOI 10.1016/j.chemosphere.2020.128180. - ISSN 1879-1298(eISSN)
Примечания : Cited References:101. - This study (polymer synthesis and investigation) was financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 074-02-2018-328) in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning", and by the State assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (investigation of polymer degradation in soils of Evenkia).
Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic - poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9-11 degrees C, the total biomass of microorganisms at a 10-20-cm depth was 226-234 mg g(-1) soil and CO2 production was 20-46 mg g(-1)W day(-1). The total abundance of microscopic fungi varied between (7.4 +/- 2.3) x 10(3) and (18.3 +/- 2.2) x 10(3) CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 +/- 0.1) x 10(6) CFU g(-1) soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique e plating of isolates on the mineral agar with polymer as sole carbon source e was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. (C) 2020 Elsevier Ltd. All rights reserved.
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14.

Вид документа : Статья из сборника (выпуск монографической серии)
Шифр издания :
Автор(ы) : Stolyar, S., V, Chekanova L. A., Yaroslavtsev R. N., Ladygina V. P., Tirranen L. S.
Заглавие : Effect of nanoparticles in growth of test - bacteria
Колич.характеристики :6 с
Место публикации : INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED PHYSICS, INFORMATION: IOP PUBLISHING LTD, 2019. - Vol. 1399: International Scientific Conference on Applied Physics, Information (SEP 25-27, 2019, Siberian Fed Univ, Polytechn Inst, Krasnoyarsk, RUSSIA). - Ст.022029. - (Journal of Physics Conference Series). - , DOI 10.1088/1742-6596/1399/2/022029
Примечания : Cited References:10
Аннотация: Confident effect of five magnetic composite nanoparticles (FeP@Ag, FeP@Pd, CoP, NiP, Fe2O3@AF) on growth of test bacteria colonies (Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus) in five replicates each is considered. Reliable inhibitors of colonies of all five test bacteria were nanoparticles FeP@Ag. CoP nanoparticles are reliable inhibitors of growth of 4 test bacteria (except for test bacteria Escherichia coli). NiP nanoparticles are reliable inhibitors of growth of 2 test bacteria: Escherichia coli and Klebsiella pneumoniae. Bacteria Escherichia coli were most sensitive to the effect of magnetic nanoparticles; and bacteria Pseudomonas aeruginosa and Staphylococcus aureus were most resistant to the effect of magnetic nanoparticles. The prospects of the method are in the possibility of multiple reuse of the magnetic particles with antimicrobial properties for bacterial decontamination of the studied sources of water and removal of magnetic nanoparticles from the treated liquids by electromagnet. The method can find use in water treatment facilities for household, Industrial and medical wastes.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prudnikova S. V., Evgrafova S. Y., Volova T. G.
Заглавие : Metabolic activity of cryogenic soils in the subarctic zone of Siberia towards “green” bioplastics
Место публикации : Chemosphere: Elsevier Ltd, 2021. - Vol. 263. - Ст.128180. - ISSN 00456535 (ISSN), DOI 10.1016/j.chemosphere.2020.128180
Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic – poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9–11 °C, the total biomass of microorganisms at a 10-20-cm depth was 226–234 mg g?1 soil and CO2 production was 20–46 mg g?1 day?1. The total abundance of microscopic fungi varied between (7.4 ± 2.3) ? 103 and (18.3 ± 2.2) ? 103 CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 ± 0.1) ? 106 CFU g?1 soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique – plating of isolates on the mineral agar with polymer as sole carbon source – was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)-degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. © 2020 Elsevier Ltd
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova, Tatiana, Zhila, Natalia, Vinogradova, Olga, Shumilova, Anna, Prudnikova, Svetlana, Shishatskaya, Ekaterina
Заглавие : Characterization of biodegradable poly-3-hydroxybutyrate films and pellets loaded with the fungicide tebuconazole
Колич.характеристики :12 с
Коллективы : Russian Science Foundation [14-26-00039]
Место публикации : Environ. Sci. Pollut. Res.: SPRINGER HEIDELBERG, 2016. - Vol. 23, Is. 6. - С. 5243-5254. - ISSN 0944-1344, DOI 10.1007/s11356-015-5739-1. - ISSN 1614-7499(eISSN)
Примечания : Cited References:33. - This study was supported by the Russian Science Foundation (grant no. 14-26-00039).
Предметные рубрики: CONTROLLED-RELEASE
MICROBIAL-DEGRADATION
BRASSICA-NAPUS
OILSEED RAPE
Ключевые слова (''Своб.индексиров.''): poly(3-hydroxybutyrate)--tebuconazole--slow-release formulations--controlled release--antifungal activity
Аннотация: Biodegradable polymer poly(3-hydroxybutyrate) (P3HB) has been used as a matrix to construct slow-release formulations of the fungicide tebuconazole (TEB). P3HB/TEB systems constructed as films and pellets have been studied using differential scanning calorimetry, X-ray structure analysis, and Fourier transform infrared spectroscopy. TEB release from the experimental formulations has been studied in aqueous and soil laboratory systems. In the soil with known composition of microbial community, polymer was degraded, and TEB release after 35 days reached 60 and 36 % from films and pellets, respectively. That was 1.23 and 1.8 times more than the amount released to the water after 60 days in a sterile aqueous system. Incubation of P3HB/TEB films and pellets in the soil stimulated development of P3HB-degrading microorganisms of the genera Pseudomonas, Stenotrophomonas, Variovorax, and Streptomyces. Experiments with phytopathogenic fungi F. moniliforme and F. solani showed that the experimental P3HB/TEB formulations had antifungal activity comparable with that of free TEB.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mogil'naya O.A., Krivomazova E.S., Kargatova T.V., Lobova T.I., Popova L.Y.
Заглавие : Formation of structured communities by natural and transgenic naphthalene-degrading bacteria
Колич.характеристики :6 с
Место публикации : Appl. Biochem. Microbiol.: MAIK NAUKA/INTERPERIODICA, 2005. - Vol. 41, Is. 1. - P63-68. - ISSN 0003-6838, DOI 10.1007/s10438-005-0012-x
Примечания : Cited References: 18
Предметные рубрики: GENETICALLY-ENGINEERED MICROORGANISM
POLYCYCLIC AROMATIC-HYDROCARBONS
BIOFILM FORMATION
DEGRADATION
Аннотация: This study concerns the formation of structured communities by monocultures and binary associations of Pseudomonas fluorescens transgenic strains and natural heterotrophic bacterial species in naphthalene-containing media with various osmotic pressures. It was shown that cells of P. fluorescens strain 5RL, harboring a recombinant construct in the chromosome, were more resistant to the combined action of the stress factors under study than P. fluorescens 82/pUTK21, harboring a recombinant construct within a plasmid. Natural P. fluorescens 1 strains, particularly Vibrio sp. 14, were more viable at high osmotic pressures and naphthalene concentrations. Experiments with the combined introduction of transgenic and natural bacterial strains at high osmotic pressures demonstrated the stable coexistence of bacterial associations in biofilms, independent of naphthalene concentration. Strains considered for introduction into the environment for bioremediation should be assessed with regard to their susceptibility to the combined effect of anthropogenic and natural stress factors. The design of bacterial associations for the same purpose should take into account the effect of factors important for their survival in polluted areas.
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18.

Вид документа : Статья из сборника (выпуск продолж. издания)
Шифр издания :
Автор(ы) : Stolyar S. V., Chekanova L. A., Yaroslavtsev R. N., Ladygina V. P., Tirranen L. S.
Заглавие : Effect of nanoparticles in growth of test - Bacteria
Место публикации : Journal of Physics: Conference Series: Institute of Physics Publishing, 2019. - Vol. 1399: International Scientific Conference on Applied Physics, Information Technologies and Engineering 2019, APITECH 2019 (25 September 2019 through 27 September 2019, ) Conference code: 156053, Is. 2. - Ст.022029. - , DOI 10.1088/1742-6596/1399/2/022029
Аннотация: Confident effect of five magnetic composite nanoparticles (FeP@Ag, FeP@Pd, CoP, NiP, Fe2O3@A) on growth of test bacteria colonies (Acinetobacter baumannii, scherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus) in five replicates each is considered. Reliable inhibitors of colonies of all five test bacteria were nanoparticles FeP@Ag. CoP nanoparticles are reliable inhibitors of growth of 4 test bacteria (except for test bacteria Escherichia oli). NiP nanoparticles are reliable inhibitors of growth of 2 test bacteria: Escherichia oli and Klebsiella pneumoniae. Bacteria Escherichia oli were most sensitive to the effect of magnetic nanoparticles; and bacteria Pseudomonas aeruginosa and Staphylococcus aureus were most resistant to the effect of magnetic nanoparticles. The prospects of the method are in the possibility of multiple reuse of the magnetic particles with antimicrobial properties for bacterial decontamination of the studied sources of water and removal of magnetic nanoparticles from the treated liquids by electromagnet. The method can find use in water treatment facilities for household, Industrial and medical wastes. © Published under licence by IOP Publishing Ltd.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova, Tatiana G., Prudnikova, Svetlana V., Vinogradova, Olga N., Syrvacheva, Darya A., Shishatskaya, Ekaterina I.
Заглавие : Microbial Degradation of Polyhydroxyalkanoates with Different Chemical Compositions and Their Biodegradability
Колич.характеристики :15 с
Коллективы : Russian Science Foundation [14-26-00039]
Место публикации : Microb. Ecol.: SPRINGER, 2017. - Vol. 73, Is. 2. - С. 353-367. - ISSN 0095-3628, DOI 10.1007/s00248-016-0852-3. - ISSN 1432-184X(eISSN)
Примечания : Cited References:65. - This study was supported by the Russian Science Foundation (Grant No. 14-26-00039).
Предметные рубрики: TROPICAL COASTAL WATERS
PHA-DEGRADING BACTERIA
ALCALIGENES-EUTROPHUS
Ключевые слова (''Своб.индексиров.''): degradable polyhydroxyalkanoates--pha--soil--pha-degrading--microorganisms
Аннотация: The study addresses degradation of polyhydroxyalkanoates (PHA) with different chemical compositions-the polymer of 3-hydroxybutyric acid [P(3HB)] and copolymers of P(3HB) with 3-hydroxyvalerate [P(3HB/3HV)], 4-hydroxybutyrate [P(3HB/4HB)], and 3-hydroxyhexanoate [P(3HB/3HHx)] (10-12 mol%)-in the agro-transformed field soil of the temperate zone. Based on their degradation rates at 21 and 28 A degrees C, polymers can be ranked as follows: P(3HB/4HB) P(3HB/3HHx) P(3HB/3HV) P(3HB). The microbial community on the surface of the polymers differs from the microbial community of the soil with PHA specimens in the composition and percentages of species. Thirty-five isolates of bacteria of 16 genera were identified as PHA degraders by the clear zone technique, and each of the PHA had both specific and common degraders. P(3HB) was degraded by bacteria of the genera Mitsuaria, Chitinophaga, and Acidovorax, which were not among the degraders of the three other PHA types. Roseateles depolymerans, Streptomyces gardneri, and Cupriavidus sp. were specific degraders of P(3HB/4HB). Roseomonas massiliae and Delftia acidovorans degraded P(3HB/3HV), and Pseudoxanthomonas sp., Pseudomonas fluorescens, Ensifer adhaerens, and Bacillus pumilus were specific P(3HB/3HHx) degraders. All four PHA types were degraded by Streptomyces.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pis'man T.I., Somova L.A., Pechurkin N.S.
Заглавие : Mathematical model of the interaction of components in a plant-rhizospheric microorganisms system at the higher level of carbon dioxide in atmosphere
Место публикации : Biofizika. - 2002. - Vol. 47, Is. 5. - С. 920-925. - ISSN 00063029 (ISSN)
Ключевые слова (''Своб.индексиров.''): carbon dioxide--algorithm--article--biological model--biomass--ecosystem--microbiology--physiology--plant seed--pseudomonas putida--wheat--algorithms--biomass--carbon dioxide--ecosystem--models, biological--pseudomonas putida--seeds--triticum
Аннотация: A mathematical model describing the interaction of plants and rhizospheric microorganisms on complete mineral medium at a higher CO2 level in the atmosphere was constructed. The positive effect of CO2-enrichment on the system plant--rhizospheric microorganisms was shown. The effect of rhizospheric microorganisms on plant growth at normal and high level of carbon dioxide was demonstrated. It was shown that the biomass of plant in the system is smaller than the biomass of plant growing without microorganisms. It was experimentally demonstrated that a simple ecosystem wheat--Pseudomonas putida--artificial soil develops and functions differently than its individual constituents in the case of a wheat-artificial soil system. With unlimited nutrition and a higher CO2 level (0.06%), plants with roots inoculated with microorganisms have a smaller biomass than plants that were not inoculated with microorganisms.
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