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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tikhomirova N.A., Ushakova S.A., Tikhomirov A.A., Kalacheva G.S., Gros J.-B.
Заглавие : Possibility of Salicornia europaea use for the human liquid wastes inclusion into BLSS intrasystem mass exchange
Место публикации : Acta Astronautica. - 2008. - Vol. 63, Is. 7-10. - С. 1106-1110. - ISSN 00945765 (ISSN) , DOI 10.1016/j.actaastro.2008.01.003
Ключевые слова (''Своб.индексиров.''): blss--nacl turnover--salicornia europaea--space biology--acids--amides--arsenic compounds--biochemical engineering--biochemistry--biomass--cellulose--curing--drying--environmental engineering--fatty acids--garnets--health--human engineering--lipids--mineralogy--minerals--mining--nitrates--nitrogen--plants (botany)--polysaccharides--polyvinyl alcohols--renewable energy resources--silica--silicate minerals--sodium--sodium chloride--solutions--sugar (sucrose)--sugars--waste utilization--biochemical composition--biochemical substances--bioregenerative life support systems (blss)--component conditions--crude protein (cp)--dry weight (dw)--essential fatty acids--europaea--h igh concentrations--human urine--linoleic acid (la)--linolenic--lipid content--liquid wastes--mass exchanges--mineral compositions--nitrate nitrogen--nitrogen nutrition--non saturation--physico chemical processes--plant functions--plant lipids--reduced nitrogen--salicornia europaea--soluble sugars--wide spectrum--nonmetals--amides--arsenic--biochemistry--biomass--biotechnology--cellulose--curing--drying--fatty acids--lipids--nonmetals--plants--polysaccharides--sugars
Аннотация: One of the ways of solving the problem of the human liquid wastes utilization in bioregenerative life support systems (BLSS) can be the use of halophytic vegetable plant Salicornia europaea capable of accumulating sodium chloride in rather high concentrations. Since the most specific higher plant function in BLSS, which at present cannot be substituted by physicochemical processes, appears to be the biosynthesis of a wide spectrum of nutritive substances necessary for a human, the object of the given work was the investigation of the S. europaea productivity, biochemical and mineral composition when grown under close to optimal BLSS vegetative component conditions. As the use of human urine after its preliminary physicochemical processing is supposed to be the mineral solution basis for the S. europaea cultivation, it is necessary to clear up the effect of reduced nitrogen on plants growth. Ground research was carried out. Biochemical composition of the S. europaea edible part showed that crude protein was contained in the highest degree. At that the content of crude protein (24% per dry weight) and cellulose (4.7% per dry weight) was higher in the plants grown on solutions containing amide nitrogen in comparison with the plants grown on solutions with nitrate nitrogen (15.4%-3.1% correspondingly). The water-soluble sugar contents were not high in the S. europaea edible part and depending on the nitrogen nutrition form they amounted to 1.1% (amide nitrogen) and 1.5% (nitrate nitrogen). The polysaccharide number (except cellulose) was rather higher and varied from 7.7% to 8.2%. Although the lipid content in the S. europaea plants was relatively low (7% per dry weight), it was shown that the plant lipids are characterized by a high nonsaturation degree mainly due to alpha linolenic and linoleic acids. Nitrogen nutrition form did not significantly affect the S. europaea productivity, and dry edible biomass of one plant was 8.6 g. Sodium and its concentrations predominated in the plant mineral composition and amounted in average to 9% per dry weight. Thus the S. europaea being the vegetable plant it can be the source of several biochemical substances and essential fatty acids. The present work also considers the influence of nitrate and amide forms of nitrogen on S. europaea biochemical and mineral composition. В© 2008 Elsevier Ltd. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vereshchagina T. A., Fedorchak M. A., Sharonova O. M., Fomenko E. V., Shishkina N. N., Zhizhaev A. M., Kudryavtsev A. N., Frank L. A., Anshits A. G.
Заглавие : Ni2+-zeolite/ferrosphere and Ni2+-silica/ferrosphere beads for magnetic affinity separation of histidine-tagged proteins
Место публикации : Dalton Trans. - 2016. - Vol. 45, Is. 4. - С. 1582-1592. - ISSN 14779226 (ISSN) , DOI 10.1039/c5dt03827h
Ключевые слова (''Своб.индексиров.''): alkalinity--amino acids--chemical modification--fly ash--hydrothermal synthesis--ion exchange--magnetic separation--magnetism--proteins--silica--zeolites--conventional methods--core shell structure--green fluorescent protein--histidine-tagged proteins--hydrothermal treatments--magnetic affinity--mesoporous silica--sorption capacities--nickel
Аннотация: Magnetic Ni2+-zeolite/ferrosphere and Ni2+-silica/ferrosphere beads (Ni-ferrosphere beads - NFB) of a core-shell structure were synthesized starting from coal fly ash ferrospheres having diameters in the range of 0.063-0.050 mm. The strategy of NFB fabrication is an oriented chemical modification of the outer surface preserving the magnetic core of parent beads with the formation of micro-mesoporous coverings. Two routes of ferrosphere modification were realized, such as (i) hydrothermal treatment in an alkaline medium resulting in a NaP zeolite layer and (ii) synthesis of micro-mesoporous silica on the glass surface using conventional methods. Immobilization of Ni2+ ions in the siliceous porous shell of the magnetic beads was carried out via (i) the ion exchange of Na+ for Ni2+ in the zeolite layer or (ii) deposition of NiO clusters in the zeolite and silica pores. The final NFB were tested for affinity in magnetic separation of the histidine-tagged green fluorescent protein (GFP) directly from a cell lysate. Results pointed to the high affinity of the magnetic beads towards the protein in the presence of 10 mM EDTA. The sorption capacity of the ferrosphere-based Ni-beads with respect to GFP was in the range 1.5-5.7 mg cm-3. © The Royal Society of Chemistry.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Asanova A. A., Polonskiy V. I., Manukovsky N. S., Khizhnyak S. V.
Заглавие : Fungistatic Activity of Engineered Nanoparticles
Место публикации : Nanotechnologies Russ.: Pleiades Publishing, 2018. - Vol. 13, Is. 5-6. - С. 277-280. - ISSN 19950780 (ISSN) , DOI 10.1134/S1995078018030023
Ключевые слова (''Своб.индексиров.''): cultivation--fungi--silver compounds--silver nanoparticles--titanium dioxide--bipolaris sorokiniana--engineered nanoparticles--high resistance--mycelium growth--nanoparticle concentrations--phytopathogenic fungi--pleurotus ostreatus--sio2 nanoparticles--silica nanoparticles
Аннотация: The influence of various concentrations of Ag, TiO2, and SiO2 nanoparticles on conidia germination of the phytopathogenic fungus Bipolaris sorokiniana and mycelium growth of the xylotrophic fungi Pleurotus ostreatus and Neonothopanus nambi was analyzed. It is established that a decrease in conidia germination of phytopathogenic fungus and mycelium growth of the xylotrophic fungi occurs at relatively high silver nanoparticle concentrations. The appropriate ЕС50 values for Bipolaris sorokiniana, Pleurotus ostreatus, and Neonothopanus nambi are found to be 30, 14, and 31 mg/dm3, respectively. No effect of various concentrations of TiO2 and SiO2 nanoparticles on the studied cultures of fungi is highlighted. Thus, the negative impact of nanosilver on the growth of fungi at a high concentration which is untypical for objects of the environment and a lack of this influence of titanium dioxide and silica nanoparticles testifies to the high resistance of mycelial forms of organisms to engineered nanoparticles. © 2018, Pleiades Publishing, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondarenko L. S., Kovel E. S., Kydralieva K. A., Dzhardimalieva G. I., Illes E., Tombacz E., Kicheeva A. G., Kudryasheva N. S.
Заглавие : Effects of modified magnetite nanoparticles on bacterial cells and enzyme reactions
Место публикации : Nanomaterials: MDPI AG, 2020. - Vol. 10, Is. 8. - Ст.1499. - С. 1-20. - ISSN 20794991 (ISSN), DOI 10.3390/nano10081499
Аннотация: Current paper presents biological effects of magnetite nanoparticles (MNPs). Analyzing effects of MNP’ characteristics (zeta-potential and hydrodynamic diameters) on bacteria and their enzyme reactions was the main focus. Photobacterium phosphoreum and bacterial enzymatic reactions were chosen as bioassays. Three types of MNPs were under study: bare Fe3O4, Fe3O4 modified with 3-aminopropyltriethoxysilane (Fe3O4/APTES), and humic acids (Fe3O4/HA). Effects of the MNPs were studied at a low concentration range (< 2 mg/L) and attributed to availability and oxidative activity of Fe3+, high negative surface charge, and low hydrodynamic diameter of Fe3O4/HA, as well as higher Fe3+ content in suspensions of Fe3O4/HA. Low-concentration suspensions of bare Fe3O4 provided inhibitory effects in both bacterial and enzymatic bioassays, whereas the MNPs with modified surface (Fe3O4/APTES and Fe3O4/HA) did not affect the enzymatic activity. Under oxidative stress (i.e., in the solutions of model oxidizer, 1,4-benzoquinone), MNPs did not reveal antioxidant activity, moreover, Fe3O4/HA demonstrated additional inhibitory activity. The study contributes to the deeper understanding of a role of humic substances and silica in biogeochemical cycling of iron. Bioluminescence assays, cellular and enzymatic, can serve as convenient tools to evaluate bioavailability of Fe3+ in natural dispersions of iron-containing nanoparticles, e.g., magnetite, ferrihydrite, etc. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondarenko, Lyubov S., Kovel, Ekaterina S., Kydralieva, Kamila A., Dzhardimalieva, Gulzhian, I, Illes, Erzsebet, Tombacz, Etelka, Kicheeva, Arina G., Kudryasheva, Nadezhda S.
Заглавие : Effects of Modified Magnetite Nanoparticles on Bacterial Cells and Enzyme Reactions
Колич.характеристики :20 с
Коллективы : Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [19-315-50048, 19-33-90149, 18-29-19003]
Место публикации : Nanomaterials: MDPI, 2020. - Vol. 10, Is. 8. - Ст.1499. - ISSN 2079-4991(eISSN), DOI 10.3390/nano10081499
Примечания : Cited References:83. - This research was funded by the Russian Foundation for Basic Research (#19-315-50048, #19-33-90149, and #18-29-19003).
Предметные рубрики: NATURAL ORGANIC-MATTER
HUMIC-ACID
DETOXIFICATION PROCESSES
Аннотация: Current paper presents biological effects of magnetite nanoparticles (MNPs). Relations of MNP' characteristics (zeta-potential and hydrodynamic diameters) with effects on bacteria and their enzymatic reactions were the main focus.Photobacterium phosphoreumand bacterial enzymatic reactions were chosen as bioassays. Three types of MNPs were under study: bare Fe3O4, Fe(3)O(4)modified with 3-aminopropyltriethoxysilane (Fe3O4/APTES), and humic acids (Fe3O4/HA). Effects of the MNPs were studied at a low concentration range ( 2 mg/L) and attributed to availability and oxidative activity of Fe3+, high negative surface charge, and low hydrodynamic diameter of Fe3O4/HA, as well as higher Fe(3+)content in suspensions of Fe3O4/HA. Low-concentration suspensions of bare Fe(3)O(4)provided inhibitory effects in both bacterial and enzymatic bioassays, whereas the MNPs with modified surface (Fe3O4/APTES and Fe3O4/HA) did not affect the enzymatic activity. Under oxidative stress (i.e., in the solutions of model oxidizer, 1,4-benzoquinone), MNPs did not reveal antioxidant activity, moreover, Fe3O4/HA demonstrated additional inhibitory activity. The study contributes to the deeper understanding of a role of humic substances and silica in biogeochemical cycling of iron. Bioluminescence assays, cellular and enzymatic, can serve as convenient tools to evaluate bioavailability of Fe(3+)in natural dispersions of iron-containing nanoparticles, e.g., magnetite, ferrihydrite, etc.
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