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A Glucose-Utilizing Strain, Cupriavidus euthrophus B-10646: Growth Kinetics, Characterization and Synthesis of Multicomponent PHAs [Text] / T. . Volova [et al.] // PLoS One. - 2014. - Vol. 9, Is. 2. - Ст. e87551, DOI 10.1371/journal.pone.0087551. - Cited References: 64. - This study was financially supported by Project "Biotechnologies of novel biomaterials: Innovative Biopolymers and Biomedicine Devices" (Agreement No. 11.G34.31.0013 with Amendment No. 1 of 15 February 2013) in accordance with Resolution No. 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning." The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. . - ISSN 1932-6203

РУБ Multidisciplinary Sciences
Рубрики:
RALSTONIA-EUTROPHA
   BIODEGRADABLE POLYHYDROXYALKANOATES
   AEROMONAS-HYDROPHILA
   ESCHERICHIA-COLI
   MOLECULAR-WEIGHT
   SURFACE-ENERGY
   NORTH PACIFIC
   TERPOLYESTER
   BIOSYNTHESIS
   POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE-CO-3-HYDROXYHEXANOATE)
Аннотация: This study investigates kinetic and production parameters of a glucose-utilizing bacterial strain, C. eutrophus B-10646, and its ability to synthesize PHA terpolymers. Optimization of a number of parameters of bacterial culture (cell concentration in the inoculum, physiological activity of the inoculum, determined by the initial intracellular polymer content, and glucose concentration in the culture medium during cultivation) provided cell concentrations and PHA yields reaching 110 g/L and 80%, respectively, under two-stage batch culture conditions. Addition of precursor substrates (valerate, hexanoate, propionate, c-butyrolactone) to the culture medium enabled synthesis of PHA terpolymers, P(3HB/3HV/4HB) and P(3HB/3HV/3HHx), with different composition and different molar fractions of 3HB, 3HV, 4HB, and 3HHx. Different types of PHA terpolymers synthesized by C. eutrophus B-10646 were used to prepare films, whose physicochemical and physicalmechanical properties were investigated. The properties of PHA terpolymers were significantly different from those of the P3HB homopolymer: they had much lower degrees of crystallinity and lower melting points and thermal decomposition temperatures, with the difference between these temperatures remaining practically unchanged. Films prepared from all PHA terpolymers had higher mechanical strength and elasticity than P3HB films. In spite of dissimilar surface structures, all films prepared from PHA terpolymers facilitated attachment and proliferation of mouse fibroblast NIH 3T3 cells more effectively than polystyrene and the highly crystalline P3HB.

WOS
Держатели документа:
[Volova, Tatiana
Kiselev, Evgeniy
Nikolaeva, Elena
Sukovatiy, Aleksey
Shishatskaya, Ekaterina] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia
[Volova, Tatiana
Vinogradova, Olga
Shishatskaya, Ekaterina] Siberian Fed Univ, Krasnoyarsk, Russia
[Chistyakov, Anton] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T...; Kiselev, E...; Vinogradova, O...; Nikolaeva, E...; Chistyakov, A...; Sukovatiy, A...; Shishatskaya, E...; Project "Biotechnologies of novel biomaterials: Innovative Biopolymers and Biomedicine Devices" [11.G34.31.0013]

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A glucose-utilizing strain, cupriavidus euthrophus B-10646: Growth kinetics, characterization and synthesis of multicomponent PHAs / T. Volova [et al.] // PLoS ONE. - 2014. - Vol. 9, Is. 2, DOI 10.1371/journal.pone.0087551 . - ISSN 1932-6203
Кл.слова (ненормированные):
3 hydroxybutyrate 3 hydroxyhexanoate 3 hydroxyvalerate copolymer -- 3 hydroxybutyrate 4 hydroxybutyrate 3 hydroxyvalerate copolymer -- copolymer -- gamma butyrolactone -- glucose -- hexanoic acid -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- polystyrene -- propionic acid -- unclassified drug -- valeric acid -- animal cell -- article -- bacterial growth -- bacterium culture -- cell adhesion -- cell proliferation -- crystal structure -- culture optimization -- Cupriavidus -- Cupriavidus euthrophus -- decomposition -- elasticity -- film -- glucose utilization -- kinetics -- mechanics -- melting point -- mouse -- nonhuman -- nucleotide sequence -- physical chemistry -- polymerization -- strength -- synthesis
Аннотация: This study investigates kinetic and production parameters of a glucose-utilizing bacterial strain, C. eutrophus B-10646, and its ability to synthesize PHA terpolymers. Optimization of a number of parameters of bacterial culture (cell concentration in the inoculum, physiological activity of the inoculum, determined by the initial intracellular polymer content, and glucose concentration in the culture medium during cultivation) provided cell concentrations and PHA yields reaching 110 g/L and 80%, respectively, under two-stage batch culture conditions. Addition of precursor substrates (valerate, hexanoate, propionate, ?-butyrolactone) to the culture medium enabled synthesis of PHA terpolymers, P(3HB/3HV/4HB) and P(3HB/ 3HV/3HHx), with different composition and different molar fractions of 3HB, 3HV, 4HB, and 3HHx. Different types of PHA terpolymers synthesized by C. eutrophus B-10646 were used to prepare films, whose physicochemical and physical-mechanical properties were investigated. The properties of PHA terpolymers were significantly different from those of the P3HB homopolymer: they had much lower degrees of crystallinity and lower melting points and thermal decomposition temperatures, with the difference between these temperatures remaining practically unchanged. Films prepared from all PHA terpolymers had higher mechanical strength and elasticity than P3HB films. In spite of dissimilar surface structures, all films prepared from PHA terpolymers facilitated attachment and proliferation of mouse fibroblast NIH 3T3 cells more effectively than polystyrene and the highly crystalline P3HB. Copyright: © 2014 Volova et al.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Science, Moscow, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.; Kiselev, E.; Vinogradova, O.; Nikolaeva, E.; Chistyakov, A.; Sukovatiy, A.; Shishatskaya, E.

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A growth medium for the carboxydobacterium Seliberia carboxydohydrogena / T. G. Volova, I. V. Gribovskaya // Mikrobiologiya. - 1986. - Vol. 55, Is. 1. - С. 72-76 . - ISSN 0026-3656
Кл.слова (ненормированные):
carbon monoxide -- bacterial growth -- culture medium -- nonhuman
Аннотация: The growth of the carboxydobacterium Seliberia carboxydohydrogena Z-1062 was studied at a different concentration of mineral elements under the conditions of continuous turbidostat cultivation. The chemical composition of the bacterium was determined and the dynamics of its specific growth rate and the intracellular content of mineral elements were studied when the concentration of these elements was changed in the growth medium. The synthesis of 1 g of biomass by this strain required: N, 120 mg; P, 23 mg; S, 5.5 mg; K, 1.5 mg; Mg, 3.5 mg. Changes in the residual concentrations of the elements within a broad range had no effect on the specific rate of the carboxydobacterial growth and chemical composition.

Scopus
Держатели документа:
Institut Biofiziki, SO AN SSSR, Krasnoyarsk, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Gribovskaya, I.V.

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A study on the possibility of environmental adaptation of a Bacillus subtilis strain containing a recombinant plasmid with the gene of human interferon alpha 2 [Text] / L. Y. Popova [et al.] // Microbiology. - 1997. - Vol. 66, Is. 6. - P. 637-641. - Cited References: 13 . - ISSN 0026-2617

РУБ Microbiology
Рубрики:
GENETICALLY-MODIFIED MICROORGANISMS
Кл.слова (ненормированные):
microecosystem -- Bacillus subtilis -- recombinant plasmid -- interferon -- adaptation
Аннотация: Adaptation of the Bacillus subtilis strain 2335/105 (Km(r)Inf(+)) containing a recombinant plasmid encoding the extracellular human interferon alpha 2 was studied under various conditions. Stability of the plasmid in the population of B. subtilis 2335/105 was estimated under nonselective conditions. The plasmid-free cells and cells with a low number of plasmid copies were found to accumulate progressively, constituting 80% of the population after 10 culture passages, indicating the poor competitiveness of cells carrying a high number of plasmid copies. The behavior of vegetative cells of the recombinant strain introduced into aquatic microcosms differing in trophic chain length was studied. Within the first 10 days, the lysis of vegetative cells of B. subtilis 2335/105 occurred; the number of viable spores was very low but remained constant for half a year.

WOS
Держатели документа:
Russian Acad Sci, Akademgorodok, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
State Sci Ctr VB Vektor, Sci Res Design & Technol Inst Biol Act Subst, Berdsk 633190, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Popova, L.Y.; Kargatova, T.V.; Maksimova, E.E.; Belyavskaya, V.A.

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Aliidiomarina shirensis sp nov., a halophilic bacterium isolated from Shira Lake in Khakasia, southern Siberia, and a proposal to transfer Idiomarina maris to the genus Aliidiomarina [Text] / H. H. Chiu [et al.] // Int. J. Syst. Evol. Microbiol. - 2014. - Vol. 64. - P1334-1339, DOI 10.1099/ijs.0.057851-0. - Cited References: 22. - We thank Dr Egor S. Zadereev and Dr Vladimir V. Zykov at the Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, for assistance with sampling. This study was supported by Russia Taiwan joint project funding (NSC 99-2923-B-001-001-MY3) from the National Science Council, Taiwan, the Russian Foundation for Basic Research, Grant No. 14-04-01060-a and Siberian Branch of Russian Academy of Sciences, joint Taiwan-Siberian Project No. 11. . - ISSN 1466-5026. - ISSN 1466-5034

РУБ Microbiology
Рубрики:
SHALLOW COASTAL WATER
   RIBOSOMAL-RNA GENE
   EMENDED DESCRIPTION
   PSEUDIDIOMARINA
   PHYLOTYPES
   SEQUENCE
   TAIWAN
Аннотация: Strain AIS(T), an aerobic halophilic, Gram-reaction-negative, heterotrophic bacterium isolated from the water of Shira Lake in Khakasia, southern Siberia, was characterized using a polyphasic approach. Our analysis of the 16S rRNA gene sequences showed that 'Aliidiomarina haloalkalitolerans', 'Allidiomarina sanyensis', Idiomarina maris and AIS(T) formed a distinct lineage. The sequence similarities between AIS(T) and the type strains of species of the genera Idiomarina and Aliidiomarina were 91.6-95.1 % and 94.0-96.9 %, respectively. The major isoprenoid quinone of AIS(T) was ubiquinone 8 (Q-8). Predominant cellular fatty acids were iso-C-17:0, iso-C-15:0 and summed feature 9. The genomic DNA G+C content was 45.8 mol%. It is concluded that AIS(T) represents a novel species of the genus Aliidiomarina, and the name Aliidiomarina shirensis sp. nov. is herein proposed for it. The type strain is AIST (=JCM 17761(T)=BCRC 80327(T)). Based on its fatty acid profile and our phylogenetic analysis, we propose that Idiomarina mans be transferred to the genus Aliidiomarina.

WOS
Держатели документа:
[Chiu, Hsiu-Hui
Tang, Sen-Lin] Acad Sinica, Biodivers Res Ctr, Taipei 115, Taiwan
[Rogozin, Denis Yu.
Degermendzhy, Andrei G.] Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk, Russia
[Rogozin, Denis Yu.] Siberian Fed Univ, Krasnoyarsk, Russia
[Huang, Ssu-Po
Shieh, Wung Yang] Natl Taiwan Univ, Inst Oceanog, Taipei 10764, Taiwan
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Chiu, H.H.; Rogozin, D.Y.; Huang, S.P.; Degermendzhy, A.G.; Shieh, W.Y.; Tang, S.L.; National Science Council, Taiwan [NSC 99-2923-B-001-001-MY3]; Russian Foundation for Basic Research [14-04-01060-a]; Siberian Branch of Russian Academy of Sciences, joint Taiwan-Siberian Project [11]

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Aliidiomarina shirensis sp. nov., a halophilic bacterium isolated from Shira Lake in Khakasia, southern Siberia, and a proposal to transfer Idiomarina maris to the genus Aliidiomarina [] / H. -H. Chiu [et al.] // Int. J. Syst. Evol. Microbiol. - 2014. - Vol. 64, Is. PART 4. - Ст. 057851. - P1334-1339, DOI 10.1099/ijs.0.057851-0 . - ISSN 1466-5026
Аннотация: Strain AIST, an aerobic halophilic, Gram-reaction-negative, heterotrophic bacterium isolated from the water of Shira Lake in Khakasia, southern Siberia, was characterized using a polyphasic approach. Our analysis of the 16S rRNA gene sequences showed that 'Aliidiomarina haloalkalitolerans', 'Aliidiomarina sanyensis', Idiomarina maris and AIST formed a distinct lineage. The sequence similarities between AIST and the type strains of species of the genera Idiomarina and Aliidiomarina were 91.6-95.1 % and 94.0-96.9 %, respectively. The major isoprenoid quinone of AIST was ubiquinone 8 (Q-8). Predominant cellular fatty acids were iso-C17: 0, iso-C15: 0 and summed feature 9. The genomic DNA G+C content was 45.8 mol%. It is concluded that AIST represents a novel species of the genus Aliidiomarina, and the name Aliidiomarina shirensis sp. nov. is herein proposed for it. The type strain is AIST (= JCM 17761T = BCRC 80327T). Based on its fatty acid profile and our phylogenetic analysis, we propose that Idiomarina maris be transferred to the genus Aliidiomarina. © 2014 IUMS.

Scopus
Держатели документа:
Biodiversity Research Center, Academia Sinica, Taipei, Taiwan
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Oceanography, National Taiwan University, PO Box 23-13, Taipei, Taiwan : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Chiu, H.-H.; Rogozin, D.Y.; Huang, S.-P.; Degermendzhy, A.G.; Shieh, W.Y.; Tang, S.-L.

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Analysis of river water by bioluminescent biotests / A. M. Kuznetsov, E. K. Rodicheva, S. E. Medvedeva // Luminescence. - 1999. - Vol. 14, Is. 5. - P263-265 . - ISSN 1522-7235
Кл.слова (ненормированные):
Bioluminescent bioassay -- Pollution -- Water quality -- fresh water -- article -- bioassay -- Escherichia coli -- freeze drying -- genetic procedures -- luminescence -- methodology -- Photobacterium -- Russian Federation -- sensitivity and specificity -- water pollutant -- Biological Assay -- Biosensing Techniques -- Escherichia coli -- Freeze Drying -- Fresh Water -- Luminescence -- Photobacterium -- Russia -- Sensitivity and Specificity -- Water Pollutants, Chemical
Аннотация: The bacterial bioluminescence has high sensitivity to the action of various inhibitors of biological activity. The lyophilized luminous bacteria Photobacterium phosphoreum (Microbiosensor B17 677F) and luminous strain Escherichia coli (Microbiosensor EC) from the Culture Collection IBSO were used to create bioluminescent biotests. They have been applied in ecological monitoring to determine the overall toxicity of the Yenisei and Angara Rivers and some water sources of Altai Territory. As a rule the heaviest pollution of water in studied rivers was registered near cities and settlements. The luminous bacteria biotests are simple and convenient in work, standardized and quantitative, have rapid response to actions of different substances and high sensitivity to environmental pollutants. It takes less than 30 min to do the biotest (the other biotests take 48-96 h). Copyright В© 1999 John Wiley & Sons, Ltd.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kuznetsov, A.M.; Rodicheva, E.K.; Medvedeva, S.E.

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Anoxygenic phototrophic bacterial community of Lake Shira (Khakassia) / O. N. Lunina [et al.] // Microbiology. - 2007. - Vol. 76, Is. 4. - P469-479, DOI 10.1134/S0026261707040133 . - ISSN 0026-2617
Кл.слова (ненормированные):
Anoxygenic photosynthesis -- Anoxygenic phototrophic bacteria -- Meromictic brackish lakes -- Ahrensia kielensis -- Bacteria (microorganisms) -- Chlorobi -- Chlorobium -- Chlorobium limicola -- Chromatiaceae -- Lamprocystis purpurea -- Photobacteria -- Proteobacteria -- Rhodomicrobium -- Rhodovulum -- Rhodovulum strictum -- Thiocapsa roseopersicina
Аннотация: The anoxygenic phototrophic bacterial community of the brackish meromictic Lake Shira (Khakassia) was investigated in August 2001, July 2002, and February-March 2003. In all the periods of investigation, the prevailing microorganisms were purple sulfur bacteria similar to Lamprocystis purpurea in morphology and pigment composition. Their highest number (3 ? 10 5 cells/ml) was recorded in July 2002 at the depth of 15 m. According to 16S rRNA gene analysis, the strain of purple sulfur bacteria isolated in 2001 and designated ShAm01 exhibited 98.6% similarity to the type strain of Thiocapsa roseopersicina and 97.1-94.4% similarity to the type strains of Tca. pendens, Tca. litoralis, and Tca. rosea. The minor microorganisms of the anoxygenic phototrophic bacterial community within the period of investigation were nonsulfur purple bacteria phylogenetically close to Rhodovulum strictum (98.3% similarity, strain ShRb01), Ahrensia kielensis (of 93.9% similarity, strain ShRb02), Rhodomicrobium vannieli (of 99.7% similarity, strain ShRmc01), and green sulfur bacteria, phylogenetically close to Chlorobium limicola (of 98.7% similarity, strain ShCl03). В© 2007 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russian Federation
Skryabin Institute of Microbial Biochemistry and Physiology, Russian Academy of Sciences, Pushchino
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Lunina, O.N.; Bryantseva, I.A.; Akimov, V.N.; Rusanov, I.I.; Barinova, E.S.; Lysenko, A.M.; Rogozin, D.Yu.; Pimenov, N.V.

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Antibacterial properties of films of cellulose composites with silver nanoparticles and antibiotics / T. G. Volova [et al.] // Polym Test. - 2018. - Vol. 65. - P54-68, DOI 10.1016/j.polymertesting.2017.10.023 . - ISSN 0142-9418
Кл.слова (ненормированные):
Antibacterial activity -- Antibiotics -- Bacterial cellulose -- Composites -- Properties -- Silver nanoparticles -- Antibiotics -- Atoms -- Boron carbide -- Cell culture -- Cellulose -- Cellulose films -- Composite materials -- Escherichia coli -- Materials testing apparatus -- Metal nanoparticles -- Nanocomposite films -- Nanoparticles -- Scanning electron microscopy -- Silver compounds -- Spectrum analysis -- Synthesis (chemical) -- Tensile testing -- Water pollution -- X ray analysis -- Anti-bacterial activity -- Antibacterial properties -- Bacterial cellulose -- Mechanical characteristics -- Properties -- Silver nanoparticles -- Structure and properties -- Tensile testing machines -- Silver -- Antibiotics -- Cellulose -- Composites -- Properties -- Silver
Аннотация: The present study describes production of bacterial cellulose composites with silver nanoparticles and antibiotics and compares their properties. Bacterial cellulose (BC) composites synthesized in the culture of the strain of acetic acid bacterium Komagataeibacter xylinus VKPM B-12068 with silver nanoparticles, BC/AgNps, were produced hydrothermally, under different AgNO3 concentrations (0.0001, 0.001, and 0.01 M) in the reaction medium. The presence of silver in the BC/AgNp composites was confirmed by elemental analysis conducted using scanning electron microscopy with a system of X-ray spectral analysis. Analysis showed that the average atomic number of silver particles in composite samples depended on the concentration of AgNO3: as AgNO3 concentration in the reaction solution was increased, silver content in the composites increased from 0.044 to 0.37 mg/cm2. BC composites with amikacin and ceftriaxone were prepared by immersing dry BC films in solutions containing different concentrations of the antibiotics. The surface structure and properties and physicochemical and mechanical characteristics of composites were investigated using SEM, DSC, X-ray analysis, the system for measuring water contact angles, and electromechanical tensile testing machine. The disk-diffusion method and the shake-flask culture method used in this study showed that all experimental composites had pronounced antibacterial activity against E. coli, Ps. eruginosa, K. pneumoniae, and St. aureus, and the BC/antibiotic composites were more active than BC/AgNp ones; S. aureus was the most susceptible to the effect of BC composites. No potential cytotoxicity was detected in any of the BC/AgNp composites in the NIH 3T3 mouse fibroblast cell culture, in contrast to the BC/antibiotic composites. These results suggest that BC composites constructed in the present study hold promise as dressings for managing wounds, including contaminated ones. © 2017 Elsevier Ltd

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Держатели документа:
Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk, Russian Federation
Kirensky Institute of Physics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 43/50 Akademgorodok, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Volova, T. G.; Shumilova, A. A.; Shidlovskiy, I. P.; Nikolaeva, E. D.; Sukovatiy, A. G.; Vasiliev, A. D.; Shishatskaya, E. I.

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10.

Architectonics of Flavobacterium sp. 56 and Flavobacterium sp. 22 colonies as exposed by transmission electron microscopy [Text] / A. P. Puzyr', O. A. Mogil'naya, L. S. Tirranen // Microbiology. - 1998. - Vol. 67, Is. 5. - P. 555-562. - Cited References: 21 . - ISSN 0026-2617

РУБ Microbiology
Рубрики:
BACTERIAL
Кл.слова (ненормированные):
electron microscopy -- colony architectonics -- Flavobacterium
Аннотация: Colonies of Flavobacterium sp. 56 and Flavobacterium sp. 22 were studied by electron microscopy without being washed off from solid nutrient medium and were shown to differ in the spatial arrangement and ultrastructure of their cells. Colonies of Flavobacterium sp. 56 contained a single type of cells, while those of Flavobacterium sp. 22 consisted of cells of two types differing in their ultrastructure. Within the colony volume, bacteria were oriented in a regular pattern characteristic of each strain studied. The evidence obtained relating to the ultrastructure, spatial orientation, and location of cells is sufficient for the unambiguous identification of bacterial strains and provides new information on colony architectonics.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Puzyr', A.P.; Mogil'naya, O.A.; Tirranen, L.S.

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11.

Autochthonous microbial cenosis as a potential source of antagonistic strains for biological struggle against wheat fusarium in biotechnical life support systems / S. V. Khizhnyak, S. A. Petrushkina, V. E. Chernov [и др.] // Aviakosmicheskaya Ekol. Med. - 2020. - Vol. 54, Is. 3. - С. 84-91, DOI 10.21687/0233-528X-2020-54-3-84-91 . - ISSN 0233-528X
Кл.слова (ненормированные):
Biological protection of plants against diseases -- Biotechnical life support systems -- Fusarium -- Wheat
Аннотация: The paper dwells upon the use of autochthonous microbial cinosis as a source of antagonistic strains for bioprotection of wheat against Fusarium in biotechnical life support systems (BT LSS). Six bacterial strains antagonistic to 9 phytopathogenic fungi Fusarium responsible for seedling blight of wheat in BT LSS were isolated from hydroponic solutions and artificial soils and subject to the genetic typing. Five strains represent bacteria g. Bacillus, one strain - Chryseobacterium. The 16S rRNA sequencing showed 94 to 100 % genetic affinity with typical cultures. All spore-forming strains-antagonists were capable of germinating and going through the whole development cycle In the presence of swelling wheat seeds. The isolates did not demonstrate antagonism to each other and can grow in a mixed culture. Spectra and levels of the antiobiotic activity of the antagonists, along with the Fusarium sensitivity to their antibiotic action, differed statistically (p < 0.001). None of the antagonists was capable of suppressing the entire Fusarium spectrum detected in BT LSS individually. At the same time, treatment of seeds with a mixture of antagonists suppressed totally fungal development in wheat artificially inoculated by Fusarium conidia from a BT LSS roll culture, and had a statistical stimulating effect (p = 0.01) on seedlings. © 2020 Slovo Ltd. All rights reserved.

Scopus
Держатели документа:
Krasnoyarsk State Agrarian University, Russian Federation
Military Medical Academy named after S.M. Kirov, St. Petersburg, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center «Krasnoyarsk Science Center SB RAS», Russian Federation
Reshetnev Siberian State University of Science and Technology, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Khizhnyak, S. V.; Petrushkina, S. A.; Chernov, V. E.; Ushakova, S. A.; Tikhomirov, A. A.

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12.

Autotrophic synthesis of polyhydroxyalkanoates by the bacteria Ralstonia eutropha in the presence of carbon monoxide [Text] / T. G. Volova, G. S. Kalacheva, O. V. Altukhova // Appl. Microbiol. Biotechnol. - 2002. - Vol. 58, Is. 5. - P. 675-678, DOI 10.1007/s00253-002-0941-8. - Cited References: 35 . - ISSN 0175-7598

РУБ Biotechnology & Applied Microbiology
Рубрики:
PSEUDOMONAS-OLEOVORANS
   ALCALIGENES-EUTROPHUS
   COAL
   CULTIVATION
   METABOLISM
   SUBSTRATE
   CULTURE
   CO2
   H-2
   O-2
Аннотация: It has been found that the carbon monoxide (CO)-resistant strain of the hydrogen bacteria Ralstonia eutropha B5786 is able to synthesise polyhydroxyalkanoates (PHAs) in the presence of CO under autotrophic conditions. This strain, grown on model gas mixtures containing 5-25% CO v/v), accumulates up to 70-75% (of absolutely dry matter) PHA, without significant variation in the yield coefficient on hydrogen. No suppression of the activities of the key enzymes of PHA synthesis (beta-ketothiolase, acetoacetyl-CoA-reductase, butyrate dehydrogenase and poly-3-hydroxybutyrate synthase) was recorded. The PHA synthesised is a copolymer containing mostly beta-hydroxybutyrate (more than 99 mol%) with trace amounts of beta-hydroxyvalerate. The investigated properties of the polymer (molecular weight, crystallinity, temperature characteristics) do not differ from those of the polymer synthesised on electrolytic hydrogen.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Altukhova, O.V.

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13.

Autotrophic synthesis of polyhydroxyalkanoates by the bacteria Ralstonia eutropha in the presence of carbon monoxide / T. G. Volova, G. S. Kalacheva, O. V. Altukhova // Applied Microbiology and Biotechnology. - 2002. - Vol. 58, Is. 5. - P675-678, DOI 10.1007/s00253-002-0941-8 . - ISSN 0175-7598
Кл.слова (ненормированные):
3 hydroxybutyric acid -- acetoacetyl coenzyme a reductase -- acetyl coenzyme A acyltransferase -- beta hydroxyvalerate -- butyrate dehydrogenase -- carbon monoxide -- electrolyte -- hydrogen -- oxidoreductase -- poly(3 hydroxybutyric acid) -- poly(3 hydroxybutyric acid)synthase -- polyhydroxyalkanoic acid -- polymer -- unclassified drug -- valeric acid -- bacterium -- article -- autotrophy -- bacterial growth -- bacterial strain -- biomass production -- controlled study -- crystallization -- enzyme activity -- molecular weight -- nonhuman -- synthesis -- temperature -- Wautersia eutropha -- Carbon Monoxide -- Culture Media -- Cupriavidus necator -- Fatty Acids -- Lipids -- Polyesters -- Bacteria (microorganisms) -- Negibacteria -- Ralstonia -- Wautersia eutropha
Аннотация: It has been found that the carbon monoxide (CO)-resistant strain of the hydrogen bacteria Ralstonia eutropha B5786 is able to synthesise polyhydroxy-alkanoates (PHAs) in the presence of CO under autotrophic conditions. This strain, grown on model gas mixtures containing 5-25% CO (v/v), accumulates up to 70-75% (of absolutely dry matter) PHA, without significant variation in the yield coefficient on hydrogen. No suppression of the activities of the key enzymes of PHA synthesis (?-ketothiolase, acetoacetyl-CoA-reductase, butyrate dehydrogenase and poly-3-hydroxybutyrate synthase) was recorded. The PHA synthesised is a copolymer containing mostly ?-hydroxybutyrate (more than 99 mol%) with trace amounts of ?-hydroxyvalerate. The investigated properties of the polymer (molecular weight, crystallinity, temperature characteristics) do not differ from those of the polymer synthesised on electrolytic hydrogen.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Br. Russian Academy of Sci., 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Altukhova, O.V.

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14.

Bacterial Cellulose (BC) and BC Composites: Production and Properties / TGG Volova, SVV Prudnikova, EGG Kiselev [et al.] // Nanomaterials. - 2022. - Vol. 12, Is. 2. - Ст. 192, DOI 10.3390/nano12020192. - Cited References:113. - This research was financially supported by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006. . - ISSN 2079-4991

РУБ Chemistry, Multidisciplinary + Nanoscience & Nanotechnology + Materials
Рубрики:
SILVER NANOPARTICLES
GLUCONACETOBACTER-HANSENII
MICROBIAL CELLULOSE
Кл.слова (ненормированные):
bacterial cellulose -- composites -- production -- properties
Аннотация: The synthesis of bacterial cellulose (BC) by Komagataeibacter xylinus strain B-12068 was investigated on various C-substrates, under submerged conditions with stirring and in static surface cultures. We implemented the synthesis of BC on glycerol, glucose, beet molasses, sprat oil, and a mixture of glucose with sunflower oil. The most productive process was obtained during the production of inoculum in submerged culture and subsequent growth of large BC films (up to 0.2 m(2) and more) in a static surface culture. The highest productivity of the BC synthesis process was obtained with the growth of bacteria on molasses and glycerol, 1.20 and 1.45 g/L per day, respectively. We obtained BC composites with silver nanoparticles (BC/AgNPs) and antibacterial drugs (chlorhexidine, baneocin, cefotaxime, and doripenem), and investigated the structure, physicochemical, and mechanical properties of composites. The disc-diffusion method showed pronounced antibacterial activity of BC composites against E. coli ATCC 25922 and S. aureus ATCC 25923.

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Держатели документа:
Siberian Fed Univ, Sch Fundamental Biol & Biotechnol, 79 Svobodny Pr, Krasnoyarsk 660041, Russia.
RAS, Krasnoyarsk Sci Ctr SB, Fed Res Ctr, Inst Biophys SB, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
RAS, Krasnoyarsk Sci Ctr SB, Fed Res Ctr, LV Kirensky Inst Phys SB, 50-38 Akademgorodok, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Siberian Branch, Krasnoyarsk Sci Ctr, Fed Res Ctr, 50 Akademgorodok, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Sch Petr & Gas Engn, 79 Svobodny Pr, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Volova, Tatiana G. G.; Prudnikova, Svetlana V. V.; Kiselev, Evgeniy G. G.; Nemtsev, Ivan V. V.; Vasiliev, Alexander D. D.; Kuzmin, Andrey P. P.; Shishatskaya, Ekaterina I. I.; Kiselev, Evgeniy; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]

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15.

Bio-hybridization of nanobactericides with cellulose films for effective treatment against members of ESKAPE multi-drug-resistant pathogens / S. Baker [et al.] // Appl. Nanosci. - 2018. - Vol. 8, Is. 5. - P1101-1110, DOI 10.1007/s13204-018-0717-9. - Cited References:51. - Authors are thankful for Ministry of Education and Science of the Russian Federation for providing funding under the scheme of 5-100: Russian Academic Excellence Project. Authors are grateful for facilities provided by Siberian Federal University to carry out the present study. . - ISSN 2190-5509. - ISSN 2190-5517

РУБ Nanoscience & Nanotechnology
Рубрики:
SILVER NANOPARTICLES
BACTERIAL CELLULOSE
ANTIBIOTIC-RESISTANCE
Кл.слова (ненормированные):
ESKAPE -- Bio-hybridization -- Silver nanobactericides -- Phytogenic -- Bactericidal activity
Аннотация: The rapid expansion of drug-resistant pathogens has created huge global impact and development of novel antimicrobial leads is one of the top priority studies in the current scenario. The present study aims to develop bio-hybridized nanocellulose films which comprise of phytogenic silver nanobactericides. The nanobactericides were synthesized by treating 1 mM silver nitrate with aqueous extract of Chamerion angustifolium which reduced the metal salt to produce polydispersed nanobactericides which were tested against the members of ESKAPE drug-resistant communities. The synthesized silver nanobactericides were subjected to characterization with UV-visible spectra which displayed maximum absorbance at 408 nm. The bio-molecular interaction of phyto-constituents to mediate synthesis and stabilization of nanobactericides was studied with Fourier-transform infrared spectroscopy (FTIR) which depicted functional groups associated with nanobactericides. The crystalline nature was studied with X-ray diffraction (XRD) which showed Bragg's intensities at 2 theta angle which denoted (111), (200), (220), and (311) planes. The morphological characteristics of silver nanobactericides were defined with transmission electron Microscopy (TEM) image which displayed polydispersity of silver nanobactericides with size ranging from 2 to 40 nm. The synthesized nanobactericides showed a significant activity against MRSA strain with 21 mm zone of inhibition. The minimal inhibitory concentration of silver nanobactericides to inhibit the growth of test pathogens was also determined which ranged between 0.625 and 1.25 mu g/ml. The silver nanobactericides were bio-hybridized onto nanocellulose films produced by Komagataeibacter xylinus B-12068 culture strain. The films were dried to determine the mechanical properties which showed increased in Young's modulus and tensile strength in comparison with control bacterial cellulose films. Overall, the results obtained in the present investigation are promising enough to report bactericidal activity of bio-hybridized nanobactericidal films against ESKAPE. These communities are reported to cause severe threats to all forms of lives irrespective to their habitats which can lead to huge economical crisis.

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Держатели документа:
Siberian Fed Univ, Lab Biotechnol New Mat, Svobodnyy Pr 79, Krasnoyarsk 660041, Russia.
SB RAS, Krasnoyarsk Sci Ctr, Fed Res Ctr, Inst Biophys, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, 79 Svobodny Pr, Krasnoyarsk 660041, Russia.
Siberian Fed Univ, Sch Fundamental Biol & Biotechnol, Krasnoyarsk, Russia.
Krasnoyasrk State Med Univ, Dept Microbiol, Krasnoyarsk Partizana Zheleznyaka St 1, Krasnoyarsk 660022, Russia.
SB RAS, Fed Res Ctr KSC, Kirensky Inst Phys, Akademgorodok 50,Bld 38, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Sch Petr & Nat Gas Engn, Krasnoyarsk, Russia.

Доп.точки доступа:
Baker, Syed; Volova, Tatiana; Prudnikova, Svetlana, V; Shumilova, Anna A.; Perianova, Olga, V; Zharkov, Sergey M.; Kuzmin, And Rey; Olga, Kondratenka; Bogdan, Kiryukhin; Shidlovskiy, Ivan P.; Potkina, Zoya K.; Khohlova, Olga Y.; Lobova, Tatiana, I; Ministry of Education and Science of the Russian Federation under the scheme of 5-100: Russian Academic Excellence Project

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16.

Biodegradable polymers - Perspectives and applications in agriculture / E. G. Kiselev, N. O. Zhila, T. G. Volova // IOP Conference Series: Earth and Environmental Science : IOP Publishing Ltd, 2021. - Vol. 689: 2020 International Conference on Germany and Russia: Ecosystems Without Borders, EcoSystConfKlgtu 2020 (5 October 2020 through 10 October 2020, ) Conference code: 167944, Is. 1. - Ст. 012036, DOI 10.1088/1755-1315/689/1/012036
Кл.слова (ненормированные):
Biodegradable polymers -- Ecosystems -- Fungi -- Glycerol -- Monounsaturated fatty acids -- Oilseeds -- Pesticides -- Substrates -- Sunflower oil -- Fenoxaprop-p-ethyl -- Natural materials -- Pesticide formulations -- Poly-3-hydroxybutyrate -- Polyhydroxyalkanoates -- Productive process -- Strategy of constructions -- Various substrates -- Palm oil
Аннотация: The paper presents a brief overview of the results of the implementation of the project "Agropreparations of the new generation: a strategy of construction and realization". The first part contains the analysis of the growth of the wild-type strain Cupriavidus necator B-10646 (formerly eutrophus) and the synthesis of polyhydroxyalkanoates by this strain on various substrates: glycerol, palm oil, Siberian oil seed, sunflower seed oils, and oleic acid. On refined glycerin, a highly productive process is implemented when scaling up, allowing to obtain 128 ± 11 g / L PHA. Evaluation of oils has shown that palm oil is the best carbon substrate. The second part presents the results of the development of environmentally friendly slow-release pesticide formulations. They are a degradable matrix of poly-3-hydroxybutyrate mixed with natural materials (peat, clay, wood flour), into which a pesticide (metribuzin, tribenuron-methyl, fenoxaprop-P-ethyl, azoxystrobin, epoxiconazole, and tebuconazole) has been. The developed preparations showed high activity against pathogenic fungi and weeds and had a much weaker negative effect on the soil microflora. Studies of the degradation of the developed preparations and the release of pesticides into the soil confirm their effectiveness over a long period of time, up to 90 days. © Published under licence by IOP Publishing Ltd.

Scopus
Держатели документа:
School of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation
Laboratory of Chemoautotrophic Biosynthesis, Institute of Biophysics, SB, RAS, Federal Research Center, Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Kiselev, E. G.; Zhila, N. O.; Volova, T. G.

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17.

Biofilm formation by bacterial associations under various salinities and copper ion stress [Text] / O. A. Mogilnaya [et al.] // Biofouling. - 2005. - Vol. 21, Is. 05.06.2013. - P. 247-255, DOI 10.1080/08924010500445848. - Cited References: 24 . - ISSN 0892-7014

РУБ Biotechnology & Applied Microbiology + Marine & Freshwater Biology
Рубрики:
HEAVY-METAL RESISTANCE
   BACILLUS-SUBTILIS
   PROTEIN
   RISK
Кл.слова (ненормированные):
binary community -- surface films -- adhesion -- copper -- stress
Аннотация: The study addresses the effect of abiotic (medium salinity and copper ions) and biotic (interactions between populations) factors on the formation of structured communities by binary associations consisting of halotolerant bacteria (Alcaligenes sp. 1-1 or Acinetobacter sp. 1-19) and a wild-type B. subtilis 2335 strain or a transgenic strain. The results showed that 250 mg l(-1) of copper ions inhibit formation of biofilms by monocultures of the tested strains. Binary associations of the strains were more resistant to high concentrations (250 mg l(-1)) of copper ions. At the lowest NaCl concentration (0.05% and 2.5%) and in the presence of copper ions, bacilli seemed to help halotolerant bacteria survive. Under increased salinity and in the presence of copper ions, structured communities developed due to halotolerant bacteria. Coexistence under stressful conditions was beneficial for the both groups of bacteria.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
TORINS Co, Krasnoyarsk, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Mogilnaya, O.A.; Lobova, T.I.; Kargatova, T.V.; Popova, L.Y.

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18.

BIOLUMBASE - The database of natural and transgenic bioluminescent organisms / S. E. Medvedeva [et al.] // Luminescence. - 2005. - Vol. 20, Is. 2. - P90-96, DOI 10.1002/bio.809 . - ISSN 1522-7235
Кл.слова (ненормированные):
Bioluminescence -- Database -- Luminous bacteria -- lux gene -- Marine -- article -- bacterial strain -- bacterium culture -- bacterium isolation -- bioluminescence -- data base -- gene construct -- medical information -- transgenics -- wide area network -- Bacteria -- Bacterial Proteins -- Databases, Factual -- Ecology -- Luminescence -- Luminescent Proteins -- Marine Biology -- Organisms, Genetically Modified -- Photobacterium -- Transgenes
Аннотация: The Institute of Biophysics SB RAS hosts and maintains a specialized collection of luminous bacteria (CCIBSO 836) containing over 700 strains isolated in various regions of the world's oceans. The culture collection is a source of lux genes and biologically active substances. The wide application of bioluminescence in medicine and ecology has given importance to analys-ing information on the structure and functioning of bioluminescence systems in natural and transgenic microorganisms, as well as on their features that are closely interrelated with bioluminescence. The aims of our BIOLUMBASE database are: gathering information on microorganisms with lux genes, their analysis and free access, and distribution of this data throughout the global network. The database includes two sections, natural and transgenic luminous microorganisms, and is updated by our own experimental results, the published literature and internet resources. For the future, a publicly available internet site for BIOLUMBASE is planned. This will list the strains and provide comprehensive information on the properties and functions of luminous bacteria, the mechanisms of regulation of bioluminescence systems, constructs with lux genes, and applications of bioluminescence in microbiology, ecology, medicine and biotechnology. It is noteworthy that this database will also be useful for evaluation of biological hazards of transgenic strains. Users will be able to carry out bibliographic and strain searches starting from any feature of interest. Copyright В© 2005 John Wiley & Sons, Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Medvedeva, S.E.; Boyandin, A.; Lankin, Y.; Kotov, D.; Rodicheva, E.; Popova, L.

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19.

Bioluminescent detection probe for tick-borne encephalitis virus immunoassay [Text] / L. P. Burakova [et al.] // Anal. Bioanal. Chem. - 2015. - Vol. 407, Is. 18. - P5417-5423, DOI 10.1007/s00216-015-8710-6. - Cited References:19. - The work was supported by the Russian Academy of Sciences, Siberian Branch, within the framework of the Interdisciplinary Integration Project No. 139 and the State budget allocated to the fundamental research at the Russian Academy of Sciences (project No. VI 57.1.1). . - ISSN 1618-2642. - ISSN 1618-2650

РУБ Biochemical Research Methods + Chemistry, Analytical
Рубрики:
COELENTERAZINE-BINDING PROTEIN
ENZYME-IMMUNOASSAY
RENILLA-MUELLERI
Кл.слова (ненормированные):
Tick-borne encephalitis virus -- Single-chain antibody -- Luciferase -- Immunoassay
Аннотация: To facilitate the detection of the tick-borne encephalitis virus (TBEV), the causative agent of one of the most severe human neuroinfections, we have developed an immunoassay based on bioluminescent hybrid protein 14D5a-Rm7 as a detection probe. The protein containing Renilla luciferase as a reporter and a single-chain variable fragment (scFv) of murine immunoglobulin to TBEV as a recognition element was constructed, produced by bacterial expression, purified, and tested. Both domains were shown to reveal their specific biological properties-affinity to the target antigen and bioluminescent activity. Hybrid protein was applied as a label for solid-phase immunoassay of the antigens, associated with the tick-borne encephalitis virus (native glycoprotein E or extracts of the infected strain of lab ticks). The assay demonstrates high sensitivity (0.056 ng of glycoprotein E; 10(4)-10(5) virus particles or 0.1 pg virions) and simplicity and is competitive with conventional methods for detection of TBEV.

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Scopus
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Inst Chem Biol & Fundamental Med, Siberian Branch, Novosibirsk 630090, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
Russian Acad Sci, Inst Poliomyelitis & Viral Encephalitides, Moscow 142782, Russia.
Res Inst Nat Foci Infect, Omsk 644080, Russia.

Доп.точки доступа:
Burakova, Ludmila P.; Kudryavtsev, Alexander N.; Stepanyuk, Galina A.; Baykov, Ivan K.; Morozova, Vera V.; Tikunova, Nina V.; Dubova, Maria A.; Lyapustin, Victor N.; Yakimenko, Valeri V.; Frank, Ludmila A.; Russian Academy of Sciences, Siberian Branch [139]; Russian Academy of Sciences [VI 57.1.1]

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20.

Biosynthesis and properties of P(3HB-co-3HV-co-3H4MV) produced by using the wild-type strain Cupriavidus eutrophus B-10646 / T. Volova [et al.] // Journal of Chemical Technology and Biotechnology. - 2018, DOI 10.1002/jctb.5763 . - Article in press. - ISSN 0268-2575
Кл.слова (ненормированные):
3-hydroxy-4-methylvalerate -- physicochemical properties -- polyhydroxyalkanoates -- synthesis -- Biochemistry -- Chemical industry -- Synthesis (chemical) -- 3-hydroxy-4-methylvalerate -- Crystalline-to-amorphous -- Degree of crystallinity -- Degrees of crystallinity -- Physicochemical property -- Polyhydroxyalkanoates -- Processing properties -- Wild-type strain -- Polymers
Аннотация: BACKGROUND: Polyhydroxyalkanoates (PHA) containing 3-hydroxy-4-methylvalerate (3H4MV) have better processing properties than other PHA types and were not prone to ageing. The main challenge in the production of these polymers is to simultaneously achieve high cell biomass, high polymer content, and high molar fraction of 3H4MV. The aim of this study was to investigate the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxy-4-methylvalerate) [P(3HB-co-3 HV-co-3H4MV)] by Cupriavidus eutrophus B-10646, synthesize polymers with different contents of 3H4MV and investigate their properties. RESULTS: It was shown that 4-methylvalerate (4MV, the precursor substrate) concentration in the culture medium must not be higher than 1 g L?1, and that 4MV must be carefully dosed, with at least 10–12 h intervals between supplementations. A series of polymers with molar fractions of 3H4MV between 2.7 and 11.3 mol% was synthesized. Degrees of crystallinity, molecular weight characteristics, and thermal properties of the polymers have been investigated as dependent on proportions of monomers. CONCLUSION: The content of 3H4MV depends on the concentration of 4MV and conditions of carbon nutrition. The molar fraction of 3H4MV had a strong effect on the crystalline to amorphous region ratio. PHA containing 3H4MV specimens synthesized in this study had a degree of crystallinity of below 50%, which remained unchanged for 2.5 years. © 2018 Society of Chemical Industry. © 2018 Society of Chemical Industry

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Держатели документа:
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
L.V. Kirenskii Institute of Physics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
Institute of Chemistry and Chemical Technology SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
International and Inter University Centre of Nanoscience and Nanotechnology, Mahatam Gandhi University, Kottyam, India

Доп.точки доступа:
Volova, T.; Menshikova, O.; Zhila, N.; Vasiliev, A.; Kiselev, E.; Peterson, I.; Shishatskaya, E.; Thomas, S.

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