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Каталог книг и продолжающихся изданий библиотеки Института биофизики СО РАН

Иностранные журналы библиотеки Института биофизики СО РАН

Отечественные журналы библиотеки Института биофизики СО РАН

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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova, Tatiana G. G., Prudnikova, Svetlana V. V., Kiselev, Evgeniy G. G., Nemtsev, Ivan V. V., Vasiliev, Alexander D. D., Kuzmin, Andrey P. P., Shishatskaya, Ekaterina I. I.
Заглавие : Bacterial Cellulose (BC) and BC Composites: Production and Properties
Колич.характеристики :24 с
Коллективы : Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Место публикации : Nanomaterials: MDPI, 2022. - Vol. 12, Is. 2. - Ст.192. - ISSN 2079-4991(eISSN), DOI 10.3390/nano12020192
Примечания : Cited References:113. - This research was financially supported by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006.
Предметные рубрики: SILVER NANOPARTICLES
GLUCONACETOBACTER-HANSENII
MICROBIAL CELLULOSE
Аннотация: The synthesis of bacterial cellulose (BC) by Komagataeibacter xylinus strain B-12068 was investigated on various C-substrates, under submerged conditions with stirring and in static surface cultures. We implemented the synthesis of BC on glycerol, glucose, beet molasses, sprat oil, and a mixture of glucose with sunflower oil. The most productive process was obtained during the production of inoculum in submerged culture and subsequent growth of large BC films (up to 0.2 m(2) and more) in a static surface culture. The highest productivity of the BC synthesis process was obtained with the growth of bacteria on molasses and glycerol, 1.20 and 1.45 g/L per day, respectively. We obtained BC composites with silver nanoparticles (BC/AgNPs) and antibacterial drugs (chlorhexidine, baneocin, cefotaxime, and doripenem), and investigated the structure, physicochemical, and mechanical properties of composites. The disc-diffusion method showed pronounced antibacterial activity of BC composites against E. coli ATCC 25922 and S. aureus ATCC 25923.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova, Tatiana G., Kiselev, Evgeniy G., Demidenko, Alexey V., Zhila, Natalia O., Nemtsev, Ivan V., Lukyanenko, Anna V.
Заглавие : Production and Properties of Microbial Polyhydroxyalkanoates Synthesized from Hydrolysates of Jerusalem Artichoke Tubers and Vegetative Biomass
Колич.характеристики :25 с
Коллективы : Project "Agropreparations of the new generation: a strategy of construction and realization" [075-15-2021-626]; State Assignment of the Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Место публикации : Polymers: MDPI, 2022. - Vol. 14, Is. 1. - Ст.132. - ISSN 2073-4360(eISSN), DOI 10.3390/polym14010132
Примечания : Cited References:93. - This study was financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No. 075-15-2021-626) in accordance with Resolution No. 220 of the Government of the Russian Federation of 9 April 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning" (polymer synthesis, properties), and by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (films production, surface properties).
Предметные рубрики: GLUCOSE-UTILIZING STRAIN
RALSTONIA-EUTROPHA
ASPERGILLUS-NIGER
ACID
Аннотация: One of the major challenges in PHA biotechnology is optimization of biotechnological processes of the entire synthesis, mainly by using new inexpensive carbon substrates. A promising substrate for PHA synthesis may be the sugars extracted from the Jerusalem artichoke. In the present study, hydrolysates of Jerusalem artichoke (JA) tubers and vegetative biomass were produced and used as carbon substrate for PHA synthesis. The hydrolysis procedure (the combination of aqueous extraction and acid hydrolysis, process temperature and duration) influenced the content of reducing substances (RS), monosaccharide contents, and the fructose/glucose ratio. All types of hydrolysates tested as substrates for cultivation of three strains-C. necator B-10646 and R. eutropha B 5786 and B 8562-were suitable for PHA synthesis, producing different biomass concentrations and polymer contents. The most productive process, conducted in 12-L fermenters, was achieved on hydrolysates of JA tubers (X = 66.9 g/L, 82% PHA) and vegetative biomass (55.1 g/L and 62% PHA) produced by aqueous extraction of sugars at 80 degrees C followed by acid hydrolysis at 60 degrees C, using the most productive strain, C. necator B-10646. The effects of JA hydrolysates on physicochemical properties of PHAs were studied for the first time. P(3HB) specimens synthesized from the JA hydrolysates, regardless of the source (tubers or vegetative biomass), hydrolysis conditions, and PHA producing strain employed, exhibited the 100-120 degrees C difference between the T-melt and T-degr, prevailing of the crystalline phase over the amorphous one (C-x between 69 and 75%), and variations in weight average molecular weight (409-480) kDa. Supplementation of the culture medium of C. necator B-10646 grown on JA hydrolysates with potassium valerate and epsilon-caprolactone resulted in the synthesis of P(3HB-co-3HV) and P(3HB-co-4HB) copolymers that had decreased degrees of crystallinity and molecular weights, which influenced the porosity and surface roughness of polymer films prepared from them. The study shows that JA hydrolysates used as carbon source enabled productive synthesis of PHAs, comparable to synthesis from pure sugars. The next step is to scale up PHA synthesis from JA hydrolysates and conduct the feasibility study. The present study contributes to the solution of the critical problem of PHA biotechnology-finding widely available and inexpensive substrates.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zhila, Natalia O., Sapozhnikova, Kristina Yu, Kiselev, Evgeniy G., Vasiliev, Alexander D., Nemtsev, Ivan, V, Shishatskaya, Ekaterina, I, Volova, Tatiana G.
Заглавие : Properties of Degradable Polyhydroxyalkanoates (PHAs) Synthesized by a New Strain, Cupriavidus necator IBP/SFU-1, from Various Carbon Sources
Колич.характеристики :19 с
Коллективы : Russian FederationRussian Federation [075-15-2021-626, 220]; State Assignment of the Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Место публикации : Polymers: MDPI, 2021. - Vol. 13, Is. 18. - Ст.3142. - ISSN 2073-4360(eISSN), DOI 10.3390/polym13183142
Примечания : Cited References:78. - This work was financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 075-15-2021-626) in accordance with Resolution No 220 of the Government of the Russian Federation of 9 April 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning" (strain isolation, polymer synthesis and investigation), and by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (study of film properties).
Предметные рубрики: RALSTONIA-EUTROPHA
POLY(3-HYDROXYBUTYRATE) PRODUCTION
PLANT OIL
ACID
Аннотация: The bacterial strain isolated from soil was identified as Cupriavidus necator IBP/SFU-1 and investigated as a PHA producer. The strain was found to be able to grow and synthesize PHAs under autotrophic conditions and showed a broad organotrophic potential towards different carbon sources: sugars, glycerol, fatty acids, and plant oils. The highest cell concentrations (7-8 g/L) and PHA contents were produced from oleic acid (78%), fructose, glucose, and palm oil (over 80%). The type of the carbon source influenced the PHA chemical composition and properties: when grown on oleic acid, the strain synthesized the P(3HB-co-3HV) copolymer; on plant oils, the P(3HB-co-3HV-co-3HHx) terpolymer, and on the other substrates, the P(3HB) homopolymer. The type of the carbon source influenced molecular-weight properties of PHAs: P(3HB) synthesized under autotrophic growth conditions, from CO2, had the highest number-average (290 +/- 15 kDa) and weight-average (850 +/- 25 kDa) molecular weights and the lowest polydispersity (2.9 +/- 0.2); polymers synthesized from organic carbon sources showed increased polydispersity and reduced molecular weight. The carbon source was not found to affect the degree of crystallinity and thermal properties of the PHAs. The type of the carbon source determined not only PHA composition and molecular weight but also surface microstructure and porosity of the polymer films. The new strain can be recommended as a promising P(3HB) producer from palm oil, oleic acid, and sugars (fructose and glucose) and as a producer of P(3HB-co-3HV) from oleic acid and P(3HB-co-3HV-co-3HHx) from palm oil.
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Вид документа : Статья из сборника (выпуск продолж. издания)
Шифр издания :
Автор(ы) : Kiselev E. G., Zhila N. O., Volova T. G.
Заглавие : Biodegradable polymers - Perspectives and applications in agriculture
Место публикации : IOP Conference Series: Earth and Environmental Science: IOP Publishing Ltd, 2021. - Vol. 689: 2020 International Conference on Germany and Russia: Ecosystems Without Borders, EcoSystConfKlgtu 2020 (5 October 2020 through 10 October 2020, ) Conference code: 167944, Is. 1. - Ст.012036. - , DOI 10.1088/1755-1315/689/1/012036
Аннотация: The paper presents a brief overview of the results of the implementation of the project "Agropreparations of the new generation: a strategy of construction and realization". The first part contains the analysis of the growth of the wild-type strain Cupriavidus necator B-10646 (formerly eutrophus) and the synthesis of polyhydroxyalkanoates by this strain on various substrates: glycerol, palm oil, Siberian oil seed, sunflower seed oils, and oleic acid. On refined glycerin, a highly productive process is implemented when scaling up, allowing to obtain 128 ± 11 g / L PHA. Evaluation of oils has shown that palm oil is the best carbon substrate. The second part presents the results of the development of environmentally friendly slow-release pesticide formulations. They are a degradable matrix of poly-3-hydroxybutyrate mixed with natural materials (peat, clay, wood flour), into which a pesticide (metribuzin, tribenuron-methyl, fenoxaprop-P-ethyl, azoxystrobin, epoxiconazole, and tebuconazole) has been. The developed preparations showed high activity against pathogenic fungi and weeds and had a much weaker negative effect on the soil microflora. Studies of the degradation of the developed preparations and the release of pesticides into the soil confirm their effectiveness over a long period of time, up to 90 days. © Published under licence by IOP Publishing Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zhila N. O., Sapozhnikova K. Yu., Kiselev E. G., Vasiliev A. D., Nemtsev I. V., Shishatskaya E. I., Volova T. G.
Заглавие : Properties of degradable polyhydroxyalkanoates (Phas) synthesized by a new strain, cupriavidus necator ibp/sfu-1, from various carbon sources
Место публикации : Polym.: MDPI, 2021. - Vol. 13, Is. 18. - Ст.3142. - ISSN 20734360 (ISSN), DOI 10.3390/polym13183142
Аннотация: The bacterial strain isolated from soil was identified as Cupriavidus necator IBP/SFU-1 and investigated as a PHA producer. The strain was found to be able to grow and synthesize PHAs under autotrophic conditions and showed a broad organotrophic potential towards different carbon sources: sugars, glycerol, fatty acids, and plant oils. The highest cell concentrations (7–8 g/L) and PHA contents were produced from oleic acid (78%), fructose, glucose, and palm oil (over 80%). The type of the carbon source influenced the PHA chemical composition and properties: when grown on oleic acid, the strain synthesized the P(3HB-co-3HV) copolymer; on plant oils, the P(3HB-co-3HV-co-3HHx) terpolymer, and on the other substrates, the P(3HB) homopolymer. The type of the carbon source influenced molecular-weight properties of PHAs: P(3HB) synthesized under autotrophic growth conditions, from CO2, had the highest number-average (290 ± 15 kDa) and weight-average (850 ± 25 kDa) molecular weights and the lowest polydispersity (2.9 ± 0.2); polymers synthesized from organic carbon sources showed increased polydispersity and reduced molecular weight. The carbon source was not found to affect the degree of crystallinity and thermal properties of the PHAs. The type of the carbon source determined not only PHA composition and molecular weight but also surface microstructure and porosity of the polymer films. The new strain can be recommended as a promising P(3HB) producer from palm oil, oleic acid, and sugars (fructose and glucose) and as a producer of P(3HB-co-3HV) from oleic acid and P(3HB-co-3HV-co-3HHx) from palm oil. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T., Sapozhnikova K., Zhila N.
Заглавие : Cupriavidus necator B-10646 growth and polyhydroxyalkanoates production on different plant oils
Место публикации : Int. J. Biol. Macromol.: Elsevier B.V., 2020. - Vol. 164. - С. 121-130. - ISSN 01418130 (ISSN), DOI 10.1016/j.ijbiomac.2020.07.095
Аннотация: The study addresses the growth of the wild-type strain Cupriavidus necator B-10646 and synthesis of polyhydroxyalkanoates by this strain on media containing plant oils with different compositions of fatty acids: palm, Siberian oilseed, and refined and unrefined sunflower seed oils. The study showed that the best carbon substrate was palm oil. Comparison of fatty acid compositions of the starting oils and unutilized residual substrates showed that C. necator B-10646 cells consumed the fatty acids from palm oil evenly while in experiments with other oils, they utilized polyenoic fatty acids first. Higher production parameters of the culture were obtained by preparation of emulsified oil medium using Tween 80 and sodium cocoyl glutamate as emulsifiers. All polyhydroxyalkanoate specimens were terpolymers that contained 3-hydroxybutyrate as the major component and minor amounts of 3-hydroxyvalerate (0.9–1.9 mol%) and 3-hydroxyhexanoate (0.5–1.1 mol%). Molecular weight of polyhydroxyalkanoate specimens depended on the type of plant oil and emulsifier. © 2020
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova, Tatiana, Sapozhnikova, Kristina, Zhila, Natalia
Заглавие : Cupriavidus necator B-10646 growth and polyhydroxyalkanoates production on different plant oils
Колич.характеристики :10 с
Коллективы : Project "Agropreparations of the new generation: a strategy of construction and realization" [074-02-2018-328]; Government of the Russian Federation; State assignment of the Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Место публикации : Int. J. Biol. Macromol.: ELSEVIER, 2020. - Vol. 164. - С. 121-130. - ISSN 0141-8130, DOI 10.1016/j.ijbiomac.2020.07.095. - ISSN 1879-0003(eISSN)
Примечания : Cited References:52. - This studywas financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 074-02-2018-328) in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning" (polymer synthesis fromplant oils), and by the State assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (polymer properties).
Предметные рубрики: FATTY-ACID-COMPOSITION
PHA SYNTHASE GENE
PALM KERNEL OIL
Аннотация: The study addresses the growth of the wild-type strain Cupriavidus necator B-10646 and synthesis of polyhydroxyalkanoates by this strain on media containing plant oils with different compositions of fatty acids: palm, Siberian oilseed, and refined and unrefined sunflower seed oils. The study showed that the best carbon substrate was palm oil. Comparison of fatty acid compositions of the starting oils and unutilized residual substrates showed that C. necator B-10646 cells consumed the fatty acids from palm oil evenly while in experiments with other oils, they utilized polyenoic fatty acids first. Higher production parameters of the culture were obtained by preparation of emulsified oil medium using Tween 80 and sodium cocoyl glutamate as emulsifiers. All polyhydroxyalkanoate specimens were terpolymers that contained 3-hydroxybutyrate as the major component and minor amounts of 3-hydroxyvalerate (0.9-1.9 mol%) and 3-hydroxyhexanoate (0.5-1.1 mol%). Molecular weight of polyhydroxyalkanoate specimens depended on the type of plant oil and emulsifier. (C) 2020 Elsevier B.V. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shen, Y.a., Chen, Sheng-Chung, Lai, Mei-Chin, Huang, Hsing-Hua, Chiu, Hsiu-Hui, Tang, Sen-Lin, Rogozin, Denis Yu, Degermendzhy, Andrey G.
Заглавие : Methanolobus halotolerans sp. nov., isolated from the saline Lake Tus in Siberia
Колич.характеристики :8 с
Коллективы : Ministry of Education, Taiwan, ROC under the ATU planMinistry of Education, Taiwan; thematic project funding of Taiwan-Russian Joint Project [NSC99-2923-B-001-001-MY3, NSC102-2923-B-001-004]; Ministry of Science and Technology, TaiwanMinistry of Science and Technology, Taiwan [MOST103/104/105/106/107-3113-M-005-001]; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [14-04-01060-a]
Место публикации : Int. J. Syst. Evol. Microbiol.: MICROBIOLOGY SOC, 2020. - Vol. 70, Is. 10. - С. 5586-5593. - ISSN 1466-5026, DOI 10.1099/ijsem.0.004453. - ISSN 1466-5034(eISSN)
Примечания : Cited References:38. - This work was supported by grants from the Ministry of Education, Taiwan, ROC under the ATU plan (to M.C.L.), by the thematic project funding of Taiwan-Russian Joint Project (NSC99-2923-B-001-001-MY3 and NSC102-2923-B-001-004 to S.L.T.), by the Ministry of Science and Technology (MOST103/104/105/106/107-3113-M-005-001 to M.C.L.), Taiwan and the Russian Foundation for Basic Research, Grant No. 14-04-01060-a (to D.Y.R. and A.G.D.).
Предметные рубрики: TAIWANENSIS SP NOV.
METHYLOTROPHIC METHANOGEN
SEDIMENTS
ACCURATE
DNA
Аннотация: A halotolerant, psychrotolerant and methylotrophic methanogen, strain SY-01(T), was isolated from the saline Lake Tus in Siberia. Cells of strain SY-01(T) were non-motile, cocci and 0.8-1.0 mu m in diameter. The only methanogenic substrate utilized by strain SY-01(T) was methanol. The temperature range of growth for strain SY-01(T) was from 4 to 40 degrees C and the optimal temperature for growth was 30 degrees C. The pH range of growth was from pH 7.2 to 9.0, with optimal growth at pH 8.0. The NaCl range of growth was 0-1.55 M with optimal growth at 0.51 M NaCl. The G+C content of the genome of strain SY-01(T) was 43.6 mol% as determined by genome sequencing. Phylogenetic analysis revealed that strain SY-01(T) was most closely related to Methanolobus zinderi SD1(T) (97.3% 16S rRNA gene sequence similarity), and had 95.5-97.2 % similarities to other Methanolobus species with valid names. Genome relatedness between strain SY-01(T) and DSM 21339(T) was computed using average nucleotide identity and digital DNA-DNA-hybridization, which yielded values of 79.7 and 21.7 %, respectively. Based on morphological, phenotypic, phylogenetic and genomic relatedness data presented here, it is evident that strain SY-01(T) represents a novel species of the genus Methanolobus, and the name Methanolobus halotolerans sp. nov. is proposed. The type strain is SY-01(T) (=BCRC AR10051(T)=NBRC 113166(T)=DSM 107642(T)).
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zhila N. O., Kalacheva G. S., Fokht V. V., Bubnova S. S., Volova T. G.
Заглавие : Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by cupriavidus necator B-10646 from mixtures of oleic acid and 3-hydroxyvalerate precursors
Место публикации : J. Sib. Fed. Univ. - Biol.: Siberian Federal University, 2020. - Vol. 13, Is. 3. - С. 331-341. - ISSN 19971389 (ISSN), DOI 10.17516/1997-1389-0320
Аннотация: Polyhydroxyalkanoates have attracted much attention as biodegradable alternative to petroleum-based synthetic plastics. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] copolymer is one of the best characterized PHA copolymers because of its high commercial potential. However, commercial use of PHAs has been limited by their high price. One approach to reducing the cost of PHA production is to use inexpensive carbon sources (fatty acids, plant oils, etc.). The aim of this work was to study synthesis of P(3HB-co-3HV) by the Cupriavidus necator B-10646 bacterium grown on oleic acid and different biochemical precursors of 3HV. Bacterial cells were grown for 72 h at 30°C and 200 rpm on an incubator shaker. Salts of propionic or valeric acids were used as precursors of 3HV. The content and the composition of the polymer were determined by gas chromatography of fatty acid methyl esters. Lipids and polymer were extracted from biomass using the method of Folch. The addition of potassium propionate and valerate did not inhibit bacterial growth and polymer synthesis, the cell concentration and polymer content reaching 9.3-9.5 g/L and 80-83%, respectively. The addition of potassium valerate or propionate led to the synthesis of (P(3HB-co-3HV)) copolymer containing 21.2 and 14.3 mol% of 3HV, respectively. The number average molecular weight (Mn) of the polymer synthesized by the bacterium on oleic acid alone was 220 kDa; the polydispersity of the polymer was 3.5. The polymer synthesized in the presence of potassium valerate and propionate was characterized by a lower Mn (156-178 kDa) and a higher polydispersity of the polymer (4.4-4.9). The main fatty acids (FA) of intracellular lipids were oleic (33.26% of the total FA) and palmitic acid (27.48% of the total FA). The addition of potassium propionate or valerate did not cause any significant changes in the composition of the FA of intracellular lipids of the strain studied. This study demonstrates the ability of C. necator B-10646 to synthesize P(3HB-co-3HV) from mixtures of oleic acid and 3HV precursors. The data obtained can be used to develop and implement an economically feasible process of the P(3HB-co-3HV) production. © Siberian Federal University. All rights reserved.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bashmakova E. E., Kudryavtsev A. N., Frank L. A.
Заглавие : Development of the method to produce functionally active recombinant streptavidin in escherichia coli cells
Место публикации : J. Sib. Fed. Univ. - Biol.: Siberian Federal University, 2020. - Vol. 13, Is. 2. - С. 218-229. - ISSN 19971389 (ISSN), DOI 10.17516/1997-1389-0324
Аннотация: Streptavidin is a homotetrameric protein produced by Streptomyces avidinii, each subunit of which binds biotin (vitamin H), forming a stable complex (Kd = 10-15 M). Streptavidin-biotin coreaction is widely used in analytical systems, for targeted delivery of compounds, for affinity purification, etc. The aim of this study was to develop a rational technique to produce functionally active recombinant streptavidin. Recombinant Escherichia coli strains producing minimal core and full-sized streptavidin variants were obtained. The E. coli BL21 Codon Plus (DE3) RIPL, as host cells, and the pET19b plasmid carrying gene of minimally-sized core (miniSAV) or full-sized (SAV) streptavidin were used. Synthesis of miniSAV results in its localization as insoluble inclusion bodies. Denatured miniSAV yield was 130 mg per liter of E. coli c ulture. T he r enaturation g ives o nly 10- 15 % of the functionally active protein. Full-sized streptavidin localizes in the cytoplasm in a soluble state, but its toxicity causes low yield of the protein (10-13 mg per liter of the culture). The induction of SAV synthesis at the end of the logarithmic stage of cell growth was found to increase the yield of SAV approximately 2-fold. The yield of functionally active protein was 30 mg per liter culture. SAV was produced practically in individual state after affine chromatography on 2-iminobiotin agarose. One molecule of full-sized streptavidin bound 3.9 biotin molecules as was shown by colorimetric analysis using HABA (4-hydroxyazobenzene-2-carboxylic acid). Both streptavidins form sandwichtype complexes with biotinylated molecules in solid-phase microassay conditions. E. coli BL21 Codon Plus (DE3) RIPL/pET19bSAV strain was stable during storage with 20 % glycerol at -70 °C, which was shown by repeated two-year reseeding. The streptavidin producing strain (E. coli BL21 Codon Plus (DE3) RIPL/pET19bSAV) is deposited in the Collection for extremophile microorganisms and type cultures (Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk), No. 3505. The method for producing functionally active recombinant streptavidin developed in this study ensures its availability for biotechnological research. © Siberian Federal University. All rights reserved.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Khizhnyak S. V., Petrushkina S. A., Chernov V. E., Ushakova S. A., Tikhomirov A. A.
Заглавие : Autochthonous microbial cenosis as a potential source of antagonistic strains for biological struggle against wheat fusarium in biotechnical life support systems
Место публикации : Aviakosmicheskaya Ekol. Med.: Slovo Ltd, 2020. - Vol. 54, Is. 3. - С. 84-91. - ISSN 0233528X (ISSN), DOI 10.21687/0233-528X-2020-54-3-84-91
Аннотация: The paper dwells upon the use of autochthonous microbial cinosis as a source of antagonistic strains for bioprotection of wheat against Fusarium in biotechnical life support systems (BT LSS). Six bacterial strains antagonistic to 9 phytopathogenic fungi Fusarium responsible for seedling blight of wheat in BT LSS were isolated from hydroponic solutions and artificial soils and subject to the genetic typing. Five strains represent bacteria g. Bacillus, one strain - Chryseobacterium. The 16S rRNA sequencing showed 94 to 100 % genetic affinity with typical cultures. All spore-forming strains-antagonists were capable of germinating and going through the whole development cycle In the presence of swelling wheat seeds. The isolates did not demonstrate antagonism to each other and can grow in a mixed culture. Spectra and levels of the antiobiotic activity of the antagonists, along with the Fusarium sensitivity to their antibiotic action, differed statistically (p 0.001). None of the antagonists was capable of suppressing the entire Fusarium spectrum detected in BT LSS individually. At the same time, treatment of seeds with a mixture of antagonists suppressed totally fungal development in wheat artificially inoculated by Fusarium conidia from a BT LSS roll culture, and had a statistical stimulating effect (p
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T., Kiselev E., Zhila N., Shishatskaya E.
Заглавие : Synthesis of Polyhydroxyalkanoates by Hydrogen-Oxidizing Bacteria in a Pilot Production Process
Место публикации : Biomacromolecules: American Chemical Society, 2019. - Article in press. - ISSN 15257797 (ISSN) , DOI 10.1021/acs.biomac.9b00295
Аннотация: The synthesis of polyhydroxyalkanoates (PHAs) was scaled up to pilot production in a 150-L fermenter on sugars (fructose and glucose) and purified and crude glycerol in a culture of the wild-type strain Cupriavidus necator B-10646. Over 60 h of cultivation, a cell concentration of 150-160 g/L was obtained on purified glycerol and glucose; cultivation on fructose and crude glycerol resulted in a cell concentration of 130 ± 10 g/L. Polymer content and yield coefficients for the biomass were similar on all substrates (80-85 wt % and 0.29-0.33 kg biomass/kg carbon substrate, respectively). Copolymers poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and poly(3-hydroxybutyrate-co-4-hydroxybutyrate) and terpolymers poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxybutyrate) having a decreased degree of crystallinity (36-46%) were first synthesized in the scaled-up process using C. necator B-10646 cultivated on glycerol. These results will provide the basis for scaling-up PHA synthesis in an organotrophic C. necator B-10646 culture. © 2019 American Chemical Society.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kotlobay A. A., Sarkisyan K. S., Mokrushina Y. A., Marcet-Houben M., Serebrovskaya E. O., Markina N. M., Somermeyer L. G., Gorokhovatsky A. Y., Vvedensky A., Purtov K. V., Petushkov V. N., Rodionova N. S., Chepurnyh T. V., Fakhranurova L. I., Guglya E. B., Ziganshin R., Tsarkova A. S., Kaskova Z. M., Shender V., Abakumov M., Abakumova T. O., Povolotskaya I. S., Eroshkin F. M., Zaraisky A. G., Mishin A. S., Dolgov S. V., Mitiouchkina T. Y., Kopantzev E. P., Waldenmaier H. E., Oliveira A. G., Oba Y., Barsova E., Bogdanova E. A., Gabaldon T., Stevani C. V., Lukyanov S., Smirnov I. V., Gitelson J. I., Kondrashov F. A., Yampolsky I. V.
Заглавие : Genetically encodable bioluminescent system from fungi
Место публикации : Proc. Natl. Acad. Sci. U. S. A.: National Academy of Sciences, 2018. - Vol. 115, Is. 50. - С. 12728-12732. - ISSN 00278424 (ISSN) , DOI 10.1073/pnas.1803615115
Ключевые слова (''Своб.индексиров.''): bioluminescence--fungal luciferase--fungal luciferin biosynthesis
Аннотация: Bioluminescence is found across the entire tree of life, conferring a spectacular set of visually oriented functions from attracting mates to scaring off predators. Half a dozen different luciferins, molecules that emit light when enzymatically oxidized, are known. However, just one biochemical pathway for luciferin biosynthesis has been described in full, which is found only in bacteria. Here, we report identification of the fungal luciferase and three other key enzymes that together form the biosynthetic cycle of the fungal luciferin from caffeic acid, a simple and widespread metabolite. Introduction of the identified genes into the genome of the yeast Pichia pastoris along with caffeic acid biosynthesis genes resulted in a strain that is autoluminescent in standard media. We analyzed evolution of the enzymes of the luciferin biosynthesis cycle and found that fungal bioluminescence emerged through a series of events that included two independent gene duplications. The retention of the duplicated enzymes of the luciferin pathway in nonluminescent fungi shows that the gene duplication was followed by functional sequence divergence of enzymes of at least one gene in the biosynthetic pathway and suggests that the evolution of fungal bioluminescence proceeded through several closely related stepping stone nonluminescent biochemical reactions with adaptive roles. The availability of a complete eukaryotic luciferin biosynthesis pathway provides several applications in biomedicine and bioengineering. © 2018 National Academy of Sciences. All Rights Reserved.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Baker, Syed, Volova, Tatiana, Prudnikova, Svetlana, V, Shumilova, Anna A., Perianova, Olga, V, Zharkov, Sergey M., Kuzmin, And Rey, Olga, Kondratenka, Bogdan, Kiryukhin, Shidlovskiy, Ivan P., Potkina, Zoya K., Khohlova, Olga Y., Lobova, Tatiana, I
Заглавие : Bio-hybridization of nanobactericides with cellulose films for effective treatment against members of ESKAPE multi-drug-resistant pathogens
Колич.характеристики :10 с
Коллективы : Ministry of Education and Science of the Russian Federation under the scheme of 5-100: Russian Academic Excellence Project
Место публикации : Appl. Nanosci.: SPRINGER HEIDELBERG, 2018. - Vol. 8, Is. 5. - С. 1101-1110. - ISSN 2190-5509, DOI 10.1007/s13204-018-0717-9. - ISSN 2190-5517(eISSN)
Примечания : Cited References:51. - Authors are thankful for Ministry of Education and Science of the Russian Federation for providing funding under the scheme of 5-100: Russian Academic Excellence Project. Authors are grateful for facilities provided by Siberian Federal University to carry out the present study.
Предметные рубрики: SILVER NANOPARTICLES
BACTERIAL CELLULOSE
ANTIBIOTIC-RESISTANCE
Ключевые слова (''Своб.индексиров.''): eskape--bio-hybridization--silver nanobactericides--phytogenic--bactericidal activity
Аннотация: The rapid expansion of drug-resistant pathogens has created huge global impact and development of novel antimicrobial leads is one of the top priority studies in the current scenario. The present study aims to develop bio-hybridized nanocellulose films which comprise of phytogenic silver nanobactericides. The nanobactericides were synthesized by treating 1 mM silver nitrate with aqueous extract of Chamerion angustifolium which reduced the metal salt to produce polydispersed nanobactericides which were tested against the members of ESKAPE drug-resistant communities. The synthesized silver nanobactericides were subjected to characterization with UV-visible spectra which displayed maximum absorbance at 408 nm. The bio-molecular interaction of phyto-constituents to mediate synthesis and stabilization of nanobactericides was studied with Fourier-transform infrared spectroscopy (FTIR) which depicted functional groups associated with nanobactericides. The crystalline nature was studied with X-ray diffraction (XRD) which showed Bragg's intensities at 2 theta angle which denoted (111), (200), (220), and (311) planes. The morphological characteristics of silver nanobactericides were defined with transmission electron Microscopy (TEM) image which displayed polydispersity of silver nanobactericides with size ranging from 2 to 40 nm. The synthesized nanobactericides showed a significant activity against MRSA strain with 21 mm zone of inhibition. The minimal inhibitory concentration of silver nanobactericides to inhibit the growth of test pathogens was also determined which ranged between 0.625 and 1.25 mu g/ml. The silver nanobactericides were bio-hybridized onto nanocellulose films produced by Komagataeibacter xylinus B-12068 culture strain. The films were dried to determine the mechanical properties which showed increased in Young's modulus and tensile strength in comparison with control bacterial cellulose films. Overall, the results obtained in the present investigation are promising enough to report bactericidal activity of bio-hybridized nanobactericidal films against ESKAPE. These communities are reported to cause severe threats to all forms of lives irrespective to their habitats which can lead to huge economical crisis.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T., Menshikova O., Zhila N., Vasiliev A., Kiselev E., Peterson I., Shishatskaya E., Thomas S.
Заглавие : Biosynthesis and properties of P(3HB-co-3HV-co-3H4MV) produced by using the wild-type strain Cupriavidus eutrophus B-10646
Место публикации : Journal of Chemical Technology and Biotechnology. - 2018. - Article in press. - ISSN 02682575 (ISSN) , DOI 10.1002/jctb.5763
Ключевые слова (''Своб.индексиров.''): 3-hydroxy-4-methylvalerate--physicochemical properties--polyhydroxyalkanoates--synthesis--biochemistry--chemical industry--synthesis (chemical)--3-hydroxy-4-methylvalerate--crystalline-to-amorphous--degree of crystallinity--degrees of crystallinity--physicochemical property--polyhydroxyalkanoates--processing properties--wild-type strain--polymers
Аннотация: BACKGROUND: Polyhydroxyalkanoates (PHA) containing 3-hydroxy-4-methylvalerate (3H4MV) have better processing properties than other PHA types and were not prone to ageing. The main challenge in the production of these polymers is to simultaneously achieve high cell biomass, high polymer content, and high molar fraction of 3H4MV. The aim of this study was to investigate the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxy-4-methylvalerate) [P(3HB-co-3 HV-co-3H4MV)] by Cupriavidus eutrophus B-10646, synthesize polymers with different contents of 3H4MV and investigate their properties. RESULTS: It was shown that 4-methylvalerate (4MV, the precursor substrate) concentration in the culture medium must not be higher than 1 g L?1, and that 4MV must be carefully dosed, with at least 10–12 h intervals between supplementations. A series of polymers with molar fractions of 3H4MV between 2.7 and 11.3 mol% was synthesized. Degrees of crystallinity, molecular weight characteristics, and thermal properties of the polymers have been investigated as dependent on proportions of monomers. CONCLUSION: The content of 3H4MV depends on the concentration of 4MV and conditions of carbon nutrition. The molar fraction of 3H4MV had a strong effect on the crystalline to amorphous region ratio. PHA containing 3H4MV specimens synthesized in this study had a degree of crystallinity of below 50%, which remained unchanged for 2.5 years. © 2018 Society of Chemical Industry. © 2018 Society of Chemical Industry
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shidlovskiy I. P., Shumilova A. A., Shishatskaya E. I., Volova T. G.
Заглавие : Properties of Bacterial Cellulose Composites with Silver Nanoparticles
Место публикации : Biophysics: Pleiades Publishing, 2018. - Vol. 63, Is. 4. - С. 519-525. - ISSN 00063509 (ISSN) , DOI 10.1134/S0006350918040188
Ключевые слова (''Своб.индексиров.''): :bacterial cellulose--antibacterial activity--composites--hydrothermal synthesis--silver nanoparticles
Аннотация: Abstract: Composites of bacterial cellulose, which were synthesized in a culture of the strain of acetic acid bacteria Komagataeibacter xylinus VKPM B-12068, with silver nanoparticles were produced hydrothermally by varying the concentrations of AgNO3 in the medium. The presence of silver in the composites was confirmed by elemental analysis. An increase in the number of silver nanoparticles in the composite from 1.08 to 9.1 wt % (from 0.044 to 0.370 mg/cm2) was shown under increasing AgNO3 concentration in the medium from 0.0001 to 0.01 M. The structure, properties of the surface, and the physicochemical properties of the composites depending on the silver content were investigated using scanning electron microscopy, differential scanning calorimetry, X-ray diffraction, and a water contact-angle measurement system. Using the disk-diffusion method, it was shown that the resulting composites have a pronounced antibacterial activity against pathogenic microflora E. coli, Ps. eruginosa, and St. aureus. © 2018, Pleiades Publishing, Inc.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T. G., Shumilova A. A., Shidlovskiy I. P., Nikolaeva E. D., Sukovatiy A. G., Vasiliev A. D., Shishatskaya E. I.
Заглавие : Antibacterial properties of films of cellulose composites with silver nanoparticles and antibiotics
Место публикации : Polym Test: Elsevier Ltd, 2018. - Vol. 65. - С. 54-68. - ISSN 01429418 (ISSN) , DOI 10.1016/j.polymertesting.2017.10.023
Ключевые слова (''Своб.индексиров.''): antibacterial activity--antibiotics--bacterial cellulose--composites--properties--silver nanoparticles--antibiotics--atoms--boron carbide--cell culture--cellulose--cellulose films--composite materials--escherichia coli--materials testing apparatus--metal nanoparticles--nanocomposite films--nanoparticles--scanning electron microscopy--silver compounds--spectrum analysis--synthesis (chemical)--tensile testing--water pollution--x ray analysis--anti-bacterial activity--antibacterial properties--bacterial cellulose--mechanical characteristics--properties--silver nanoparticles--structure and properties--tensile testing machines--silver--antibiotics--cellulose--composites--properties--silver
Аннотация: The present study describes production of bacterial cellulose composites with silver nanoparticles and antibiotics and compares their properties. Bacterial cellulose (BC) composites synthesized in the culture of the strain of acetic acid bacterium Komagataeibacter xylinus VKPM B-12068 with silver nanoparticles, BC/AgNps, were produced hydrothermally, under different AgNO3 concentrations (0.0001, 0.001, and 0.01 M) in the reaction medium. The presence of silver in the BC/AgNp composites was confirmed by elemental analysis conducted using scanning electron microscopy with a system of X-ray spectral analysis. Analysis showed that the average atomic number of silver particles in composite samples depended on the concentration of AgNO3: as AgNO3 concentration in the reaction solution was increased, silver content in the composites increased from 0.044 to 0.37 mg/cm2. BC composites with amikacin and ceftriaxone were prepared by immersing dry BC films in solutions containing different concentrations of the antibiotics. The surface structure and properties and physicochemical and mechanical characteristics of composites were investigated using SEM, DSC, X-ray analysis, the system for measuring water contact angles, and electromechanical tensile testing machine. The disk-diffusion method and the shake-flask culture method used in this study showed that all experimental composites had pronounced antibacterial activity against E. coli, Ps. eruginosa, K. pneumoniae, and St. aureus, and the BC/antibiotic composites were more active than BC/AgNp ones; S. aureus was the most susceptible to the effect of BC composites. No potential cytotoxicity was detected in any of the BC/AgNp composites in the NIH 3T3 mouse fibroblast cell culture, in contrast to the BC/antibiotic composites. These results suggest that BC composites constructed in the present study hold promise as dressings for managing wounds, including contaminated ones. © 2017 Elsevier Ltd
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Chen S. -C., Huang H. -H., Lai M. -C., Weng C. -Y., Chiu H. -H., Tang S. -L., Rogozin D. Y., Degermendzhy A. G.
Заглавие : Methanolobus psychrotolerans sp. nov., a psychrotolerant methanoarchaeon isolated from a saline meromictic lake in siberia
Место публикации : Int. J. Syst. Evol. Microbiol.: Microbiology Society, 2018. - Vol. 68, Is. 4. - С. 1378-1383. - ISSN 14665026 (ISSN) , DOI 10.1099/ijsem.0.002685
Ключевые слова (''Своб.индексиров.''): archaea--lake shira--methanogen--methanolobus--psychrotolerant--saline meromictic lake
Аннотация: A psychrotolerant, methylotrophic methanogen, strain YSF-03T, was isolated from the saline meromictic Lake Shira in Siberia. Cells of strain YSF-03T were non-motile, irregular cocci and 0.8–1.2?m in diameter. The methanogenic substrates utilized by strain YSF-03T were methanol and trimethylamine. The temperature range of growth for strain YSF-03T was from 0 to 37 °C. The optimum growth conditions were 30–37 °C, pH 7.0–7.4 and 0.17M NaCl. The G+C content of the genome of strain YSF-03T was 41.3 mol%. Phylogenetic analysis revealed that strain YSF-03T was most closely related to Methanolobus profundi MobMT (98.15% similarity in 16S rRNA gene sequence). Genome relatedness between strain YSF-03T and MobMT was computed using the Genome-to-Genome Distance Calculator and average nucleotide identity, which gave values of 23.5 and 79.3 %, respectively. Based on the morphological, phenotypic, phylogenetic and genomic relatedness data presented here, it is evident that strain YSF-03T represents a novel species of the genus Methanolobus, for which the name Methanolobus psychrotolerans sp. nov. is proposed. The type strain is YSF-03T (=BCRC AR10049T=DSM 104044T=NBRC 112514T). © 2018 IUMS.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T. G., Prudnikova S. V., Sukovatyi A. G., Shishatskaya E. I.
Заглавие : Production and properties of bacterial cellulose by the strain Komagataeibacter xylinus B-12068
Место публикации : Appl. Microbiol. Biotechnol.: Springer Verlag, 2018. - С. 1-12. - ISSN 01757598 (ISSN) , DOI 10.1007/s00253-018-9198-8
Ключевые слова (''Своб.индексиров.''): bacterial cellulose--growth conditions--komagataeibacter xylinus--biocompatibility--cell culture--cellulose--cultivation--glucose--3t3 mouse fibroblasts--bacterial cellulose--cultivation conditions--emission spectrometry--ethanol concentrations--growth conditions--komagataeibacter xylinus--physical and mechanical properties--substrates
Аннотация: A strain of acetic acid bacteria, Komagataeibacter xylinus B-12068, was studied as a source for bacterial cellulose (BC) production. The effects of cultivation conditions (carbon sources, temperature, and pH) on BC production and properties were studied in surface and submerged cultures. Glucose was found to be the best substrate for BC production among the sugars tested; ethanol concentration of 3% (w/v) enhanced the productivity of BC. Optimization of medium and cultivation conditions ensures a high production of BC on glucose and glycerol, up to 2.4 and 3.3 g/L/day, respectively. C/N elemental analysis, emission spectrometry, SEM, DTA, and X-ray were used to investigate the structure and physical and mechanical properties of the BC produced under different conditions. MTT assay and SEM showed that native cellulose membrane did not cause cytotoxicity upon direct contact with NIH 3T3 mouse fibroblast cells and was highly biocompatible. © 2018 Springer-Verlag GmbH Germany, part of Springer Nature
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T. G., Syrvacheva D. A., Zhila N. O., Sukovatiy A. G.
Заглавие : Synthesis of P(3HB-co-3HHx) copolymers containing high molar fraction of 3-hydroxyhexanoate monomer by Cupriavidus eutrophus B10646
Место публикации : J. Chem. Technol. Biotechnol. - 2016. - Vol. 91, Is. 2. - С. 416-425. - ISSN 02682575 (ISSN) , DOI 10.1002/jctb.4592
Ключевые слова (''Своб.индексиров.''): growth kinetics--physicochemical and mechanical properties--poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)--wild-type strain cupriavidus eutrophus b10646--biomaterials--biomechanics--chemical industry--cultivation--growth kinetics--mechanical properties--organic compounds--polymers--sodium--3-hydroxyhexanoate--bacterial strains--cultivation conditions--kinetic properties--physico-chemical and mechanical properties--physiological range--poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)--wild-type strain--strain
Аннотация: BACKGROUND: P(3HB-co-3HHx) copolymers are very promising biomaterials. The main challenge in the production of these polymers is to simultaneously achieve high cell biomass; high P(3HB-co-3HHx) content; and high molar fraction of 3HHx in P(3HB-co-3HHx). The most common approach to production of these copolymers is the use of recombinant bacterial strains. The purpose of this study was to optimize the process of production of P(3HB-co-3HHx) copolymers containing high molar fractions of 3HHx by using the wild-type strain Cupriavidus eutrophus B10646. RESULTS: Kinetic properties of C. eutrophus B10646 were studied during cultivation of the cells on substrates necessary for P(3HB-co-3HHx) synthesis: glucose, nitrogen, sodium hexanoate, and sodium acrylate. The physiological ranges of their effects were determined experimentally, and C. eutrophus B10646 was grown in culture media with different dosages of these substrates. P(3HB-co-3HHx) copolymers with different molar fractions of 3HHx, including high ones (12 to 68%), were synthesized, and their physicochemical and mechanical properties were investigated. CONCLUSION: For the first time, cultivation conditions of Cupriavidus eutrophus B10646 enabled production of high biomass yields (5-6gL-1) and high content of the polymer (60-75%) that contained high 3HHx molar fraction. By varying the 3HB/3HHx ratio, one can change physicochemical and mechanical properties of P(3HB-co-3HHx) copolymers. © 2014 Society of Chemical Industry © 2016 Society of Chemical Industry.
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