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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Popova L.Y.
Заглавие : Salt-dependent inhibition of light emitting of the luminescent microorganism Escherichia coli Z9051
Колич.характеристики :5 с
Место публикации : Biofizika: MEZHDUNARODNAYA KNIGA, 2001. - Vol. 46, Is. 2. - P251-255. - ISSN 0006-3029
Примечания : Cited References: 12
Ключевые слова (''Своб.индексиров.''): bacterial luminescence--recombinant plasmid--salt concentration
Аннотация: The influence of some mineral salts on the recombinant strain Escherichia coli Z9051 was investigated. It was shown that the composition (NaCl, Na2SO4, MgCl2 and MgSO4) and concentration (5 and 10%) of the salts substantially affect the expression of genes for the luminescence system of fight-emitting bacteria cloned in the plasmid under the control of the lac-promoter. In some cases, the luminescence level of the microorganism in the presence of salts was similar to the luminescence level under catabolite repression by glucose, the more strong influence of the salts exceeding the effect of catabolite repression. The possibility of adaptation of the genetically modified microorganism to the salinity factor is discussed.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kargatova T.V., Maksimova E.E., Popova L.Y.
Заглавие : Coexistence of genetically engineered Escherichia coli strains and natural microorganisms in experimental aquatic microcosms
Колич.характеристики :6 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 2001. - Vol. 70, Is. 2. - P211-216. - ISSN 0026-2617, DOI 10.1023/A:1010437731920
Примечания : Cited References: 17
Предметные рубрики: SURVIVAL
BACTERIA
GROWTH
Ключевые слова (''Своб.индексиров.''): escherichia coli--aquatic microecosystems--recombinant plasmid
Аннотация: In experimental aquatic microcosms (AMCs), the population of the Escherichia coli strain Z905 harboring the recombinant plasmid pPHL7 (Ap(r)Lux(+)) was found to gradually accumulate AMC-adapted cells, which retained the plasmid but differed from the original cells in some biochemical and physiological characteristics. Both the original and the AMC-adapted E. coil cells could coexist with the native AMC microflora for one year or longer. When introduced into AMCs together with native pseudomonads, the AMC-adapted E. coil Z905-33 (pPHL7) cells were more competitive than the nonadapted cells.
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Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Maksimova E.E., Popova L.Y.
Заглавие : Expression of cloned genes of transgenic microorganisms introduced into man-made ecosystems
Колич.характеристики :6 с
Место публикации : SPACE LIFE SCIENCES: CLOSED ECOLOGICAL SYSTEMS: EARTH AND SPACE APPLICATIONS. Ser. ADVANCES IN SPACE RESEARCH: ELSEVIER SCIENCE BV, 2001. - Vol. 27: F4 4 Symposium of COSPAR Scientific Commission F held at the 33rd COSPAR Scientific Assembly (JUL, 2000, WARSAW, POLAND), Is. 9. - P1581-1586. - ISBN 0273-1177, DOI 10.1016/S0273-1177(01)00249-6
Примечания : Cited References: 17
Предметные рубрики: MICROCOSMS
BACTERIA
Аннотация: Modeling of transgenic microorganism introduction into small man-made ecosystems can help forecast changes in expression of cloned genes under different conditions of existence. Introduction of the E. coli Z905/pPHL7 strain containing a plasmid with luminescent system genes of luminous bacteria led to changes in cell and colony morphology, reduction in metabolic activity of cells, and, as a result, a lower level of expression of cloned gene. A low concentration of nutrients has been shown to favor greatly the phenotypic change of cells of the recombinant strain. Expression of cloned genes changed due to: a lower concentration of plasmid DNA, a change in regulation of cloned genes, and a change in cells of biosynthesis of substrates needed for expression of luminescent genes. The conducted investigations can provide a basis for the use of marker transgenic microorganisms in closed ecosystems of different types. (C) 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kargatova T.V., Maksimova E.E., Krylova T.Y., Popova L.Y.
Заглавие : Comparative characterization of the growth of recombinant Escherichia coli strains and species of the aboriginal microflora of aquatic microecosystems under selective and nonselective conditions
Колич.характеристики :6 с
Место публикации : Izv. Akad. Nauk Ser. Biol.: MEZHDUNARODNAYA KNIGA, 1999. - Is. 2. - P152-157. - ISSN 0002-3329
Примечания : Cited References: 9
Предметные рубрики: GENETICALLY-MODIFIED MICROORGANISMS
Аннотация: We have studied the kinetic characteristics of several isolates of Escherichia coli Z905 recombinant strain after introduction of the strain into model aquatic ecosystems. Most E. coli Z905 isolates grown as batch cultures under selective conditions (0.5 mu g/ml ampicillin) showed better kinetic characteristics of growth than did related species of native microflora, which originally populated laboratory microcosms.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petrushkov V.N., Gibson B.G., Visser AJWG, Lee J...
Заглавие : Purification and ligand exchange protocols for antenna proteins from bioluminescent bacteria
Колич.характеристики :17 с
Место публикации : Methods Enzymol.: ACADEMIC PRESS INC, 2000. - Vol. 305. - P164-180. - ISSN 0076-6879
Примечания : Cited References: 18
Предметные рубрики: YELLOW FLUORESCENT PROTEIN
FISCHERI STRAIN Y-1
AMINO-ACID-SEQUENCE
VIBRIO-FISCHERI
PHOTOBACTERIUM-LEIOGNATHI
RIBOFLAVIN PROTEIN
LUMINOUS BACTERIUM
LUMAZINE PROTEIN
FMN
Y1
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Altukhova O.V.
Заглавие : The autotrophic synthesis of polyhydroxyalkanoate by Alcaligenes eutrophus in the presence of carbon monoxide
Колич.характеристики :7 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 2001. - Vol. 70, Is. 6. - P640-646. - ISSN 0026-2617, DOI 10.1023/A:1013175413013
Примечания : Cited References: 19
Ключевые слова (''Своб.индексиров.''): hydrogen-oxidizing bacteria--polyhydroxyalkanoates (phas)--synthesis--co inhibition
Аннотация: The CO-resistant strain B5786 of the hydrogen-oxidizing bacterium Alcaligenes eutrophus was found to be able to synthesize polyhydroxyalkanoates (PHAs) under the conditions of growth limitation by nitrogen deficiency (the factor that promotes PHA synthesis) and growth inhibition by carbon monoxide. The gas mixtures that contained from 5 to 20 vol % CO did not inhibit the key enzymes of PHA synthesis-beta-ketothiolase. acetoacetyl-CoA reductase, hydroxybutyrate dehydrogenase, and PHA synthase. In the presence of CO, cells accumulated up to 70-75 wt % PHA (with respect to the dry biomass) without any noticeable increase in the consumption of the gas substrate. Chromatographic-mass spectrometric analysis showed that the PHA synthesized by A. eutrophus is a copolymer containing more than 99 mol % beta-hydroxybutyrate and trace amounts of beta-hydroxyvalerate. The PHA synthesized under the conditions described did not differ from that synthesized by A. eutrophus cells from electrolytic hydrogen.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Zhila N.O.
Заглавие : Specificity of lipid composition in two Botryococcus strains, the producers of liquid hydrocarbons
Колич.характеристики :7 с
Место публикации : Russ. J. Plant Physiol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2003. - Vol. 50, Is. 5. - P627-633. - ISSN 1021-4437, DOI 10.1023/A:1025636007023
Примечания : Cited References: 17
Предметные рубрики: GREEN-ALGA BOTRYOCOCCUS
BRAUNII
CULTURES
BATCH
RACE
Ключевые слова (''Своб.индексиров.''): botryococcus--hydrocarbons--structure--mass spectra
Аннотация: The structure of liquid hydrocarbons and fatty acids produced by the green alga Botryococcus was identified. Two representatives of this alga, Botryococcus braunii Kutz, strain IPPAS H-252, introduced into culture earlier and an organism isolated for the first time from the Shira Lake, were used for this identification. Fatty acid composition of B. braunii, strain H-252, lipids was characterized by a high content of trienoic acids of C16-C18 series. The hydrocarbon composition of this strain was represented by straight-chain and branched-chain C14-C28 components; long-chain linear aliphatic C20-C27 hydrocarbons (54.4%) and 2,6,10,14-tetramethylhexadecane (20.5%) predominated among them. The strain H-252 differed in its fatty acid and hydrocarbon composition from the strains described earlier as Botryococcus braunii. The fatty acid composition of the Botryococcus isolate was represented mainly by C12-C32 saturated and monoenoic acids. The hydrocarbons formed by this isolate were represented by dienoic and trienoic components. C29 (48.9-56.3%) and C31 (11.1-16.3%) hydrocarbons predominated among the C23-C31 dienoic hydrocarbons, and C27, C29, and C31 trienoic hydrocarbons comprised 2.5-2.6% of total hydrocarbons. This type of hydrocarbons and the lipid fatty acid composition were characteristic for the race A of B. braunii.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mogil'naya O.A., Krylova T.Y., Popova L.Y.
Заглавие : The morphological characteristics and the dynamics of biofilms formed by a transgenic Bacillus subtilis strain
Колич.характеристики :2 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 2003. - Vol. 72, Is. 4. - P509-510. - ISSN 0026-2617, DOI 10.1023/A:1025065311507
Примечания : Cited References: 5
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mogilnaya O.A., Krylova T.Y., Popova L.Y.
Заглавие : Development and morphological features of biofilms formed by transgenic and wild type strains of Bacillus subtilis
Колич.характеристики :9 с
Место публикации : Microbiol. Res.: URBAN & FISCHER VERLAG, 2003. - Vol. 158, Is. 4. - P327-335. - ISSN 0944-5013, DOI 10.1078/0944-5013-00212
Примечания : Cited References: 17
Предметные рубрики: ANTIBIOTIC-RESISTANCE
MICROBIAL BIOFILMS
COMMUNITY
Ключевые слова (''Своб.индексиров.''): bacillus subtilis--biofilms--transgenic microorganisms--electron microscopy
Аннотация: The study addressed the ability of the transgenic strain (TM) B. subtilis 2335/pBMB 105 (Km(r)Inf(+)) to form biofilms on the surface of liquid media of various compositions, inoculated with vegetative cells and spores. The morphological features of these biofilms do not differ from those of the films formed by the recipient strain (WT) B. subtilis 2335 (Km(s)). However, the TM and the natural one differ in the dynamics of biofilm formation and the cellular composition of the films. Biofilms of the TM are formed earlier, develop at a higher rate, but decompose later than the films of the WT. When the medium is inoculated with vegetative cells, sporulation in the biofilms of both strains undergoes glucose repression; no such effect is observed when the medium is inoculated with spores. The TM does not form films when the medium is inoculated with spores and supplemented with glycerin and kanamycin.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Altukhova O.V.
Заглавие : Autotrophic synthesis of polyhydroxyalkanoates by the bacteria Ralstonia eutropha in the presence of carbon monoxide
Колич.характеристики :4 с
Место публикации : Appl. Microbiol. Biotechnol.: SPRINGER-VERLAG, 2002. - Vol. 58, Is. 5. - P675-678. - ISSN 0175-7598, DOI 10.1007/s00253-002-0941-8
Примечания : Cited References: 35
Предметные рубрики: PSEUDOMONAS-OLEOVORANS
ALCALIGENES-EUTROPHUS
COAL
CULTIVATION
METABOLISM
SUBSTRATE
CULTURE
CO2
H-2
O-2
Аннотация: It has been found that the carbon monoxide (CO)-resistant strain of the hydrogen bacteria Ralstonia eutropha B5786 is able to synthesise polyhydroxyalkanoates (PHAs) in the presence of CO under autotrophic conditions. This strain, grown on model gas mixtures containing 5-25% CO v/v), accumulates up to 70-75% (of absolutely dry matter) PHA, without significant variation in the yield coefficient on hydrogen. No suppression of the activities of the key enzymes of PHA synthesis (beta-ketothiolase, acetoacetyl-CoA-reductase, butyrate dehydrogenase and poly-3-hydroxybutyrate synthase) was recorded. The PHA synthesised is a copolymer containing mostly beta-hydroxybutyrate (more than 99 mol%) with trace amounts of beta-hydroxyvalerate. The investigated properties of the polymer (molecular weight, crystallinity, temperature characteristics) do not differ from those of the polymer synthesised on electrolytic hydrogen.
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11.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Boyandin A.N., Lobova T.I., Popova L.Y., Pechurkin PNS
Заглавие : Survival and alteration of the plasmid-containing microorganism Escherichia coli Z905/pPHL7 introduced into manmade closed aquatic microcosms
Колич.характеристики :6 с
Место публикации : SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - P1763-1768. - ISBN 0273-1177, DOI 10.1016/S0273-1177(03)00118-2
Примечания : Cited References: 9
Предметные рубрики: MARKER
SOIL
Аннотация: It has been demonstrated that the transgenic microorganism Escherichia coli Z905/pPHL7 (Ap(r)Lux(+)) can exist for a long time at an elevated concentration of mineral salts. The microorganism was introduced into microcosms with sterile brackish water (salinity variable from 21 to 22 g l(-1)) taken from Lake Shira (Khakasia, Russia). The survival of the microorganism was estimated both by measuring the growth of the colonies on solid nutrient media and by the bioluminescence exhibited by the transgenic strain in samples from the microcosms and in the enrichment culture with the added selective factor - ampicillin (50 mug/ml). In the enrichment culture, the bioluminescent signal was registered through the 160-day experiment. It has been shown that in the closed microcosms with brackish water the E. coli strain becomes heterogeneous in its ampicillin resistance. The wpopulations of the transgenic strain were mainly represented by isolates able to persist in the medium containing 50 mug/ml, but there were also the cells (about 10%) with the threshold of ampicillin resistance not more than 0.05 mug/ml. Thus, it was shown that in the microcosms with brackish water and in the absence of the selective factor the transgenic strain survives and retains the recombinant plasmid. (C) 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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12.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Boyandin A.N., Popova L.Y.
Заглавие : Expression of lux-genes as an indicator of metabolic activity of cells in model ecosystem studies
Колич.характеристики :7 с
Место публикации : SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - P1839-1845. - ISBN 0273-1177, DOI 10.1016/S0273-1177(03)00014-0
Примечания : Cited References: 8
Аннотация: Quick response to different impacts and easy measurement make the luminescent systems of luminous bacteria an object convenient for application in various fields. Cloning of gene luminescence in different organisms is currently used to study both the survival of microbial cells and the effect of different factors on their metabolic activity, including the environment. A primary test-object in estimating bacteriological contamination of water bodies, Escherichia coli, can be conveniently used as an indicator of bactericidal properties of aquatic ecosystems. The application of Escherichia coli Z905/pPHL7 (lux(+)) as a marker microorganism can facilitate monitoring the microbiological status of closed biocenoses, including systems with higher organisms. The investigation of various parameters of microecosystems (carbon nutrition type, concentrations of inorganic ions and toxic compounds) shows that the recombinant strain E. coli Z905/pPHL7 can be effectively used as a marker. (C) 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T., Kiselev E., Vinogradova O., Nikolaeva E., Chistyakov A., Sukovatiy A., Shishatskaya E.
Заглавие : A glucose-utilizing strain, cupriavidus euthrophus B-10646: Growth kinetics, characterization and synthesis of multicomponent PHAs
Место публикации : PLoS ONE. - 2014. - Vol. 9, Is. 2. - ISSN 19326203 (ISSN) , DOI 10.1371/journal.pone.0087551
Ключевые слова (''Своб.индексиров.''): 3 hydroxybutyrate 3 hydroxyhexanoate 3 hydroxyvalerate copolymer--3 hydroxybutyrate 4 hydroxybutyrate 3 hydroxyvalerate copolymer--copolymer--gamma butyrolactone--glucose--hexanoic acid--poly(3 hydroxybutyric acid)--polyhydroxyalkanoic acid--polystyrene--propionic acid--unclassified drug--valeric acid--animal cell--article--bacterial growth--bacterium culture--cell adhesion--cell proliferation--crystal structure--culture optimization--cupriavidus--cupriavidus euthrophus--decomposition--elasticity--film--glucose utilization--kinetics--mechanics--melting point--mouse--nonhuman--nucleotide sequence--physical chemistry--polymerization--strength--synthesis
Аннотация: This study investigates kinetic and production parameters of a glucose-utilizing bacterial strain, C. eutrophus B-10646, and its ability to synthesize PHA terpolymers. Optimization of a number of parameters of bacterial culture (cell concentration in the inoculum, physiological activity of the inoculum, determined by the initial intracellular polymer content, and glucose concentration in the culture medium during cultivation) provided cell concentrations and PHA yields reaching 110 g/L and 80%, respectively, under two-stage batch culture conditions. Addition of precursor substrates (valerate, hexanoate, propionate, ?-butyrolactone) to the culture medium enabled synthesis of PHA terpolymers, P(3HB/3HV/4HB) and P(3HB/ 3HV/3HHx), with different composition and different molar fractions of 3HB, 3HV, 4HB, and 3HHx. Different types of PHA terpolymers synthesized by C. eutrophus B-10646 were used to prepare films, whose physicochemical and physical-mechanical properties were investigated. The properties of PHA terpolymers were significantly different from those of the P3HB homopolymer: they had much lower degrees of crystallinity and lower melting points and thermal decomposition temperatures, with the difference between these temperatures remaining practically unchanged. Films prepared from all PHA terpolymers had higher mechanical strength and elasticity than P3HB films. In spite of dissimilar surface structures, all films prepared from PHA terpolymers facilitated attachment and proliferation of mouse fibroblast NIH 3T3 cells more effectively than polystyrene and the highly crystalline P3HB. Copyright: © 2014 Volova et al.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Chiu H.H., Rogozin D.Y., Huang S.P., Degermendzhy A.G., Shieh W.Y., Tang S.L.
Заглавие : Aliidiomarina shirensis sp nov., a halophilic bacterium isolated from Shira Lake in Khakasia, southern Siberia, and a proposal to transfer Idiomarina maris to the genus Aliidiomarina
Колич.характеристики :6 с
Коллективы : National Science Council, Taiwan [NSC 99-2923-B-001-001-MY3]; Russian Foundation for Basic Research [14-04-01060-a]; Siberian Branch of Russian Academy of Sciences, joint Taiwan-Siberian Project [11]
Место публикации : Int. J. Syst. Evol. Microbiol.: SOC GENERAL MICROBIOLOGY, 2014. - Vol. 64. - С. 1334-1339. - ISSN 1466-5026, DOI 10.1099/ijs.0.057851-0. - ISSN 1466-5034
Примечания : Cited References: 22. - We thank Dr Egor S. Zadereev and Dr Vladimir V. Zykov at the Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, for assistance with sampling. This study was supported by Russia Taiwan joint project funding (NSC 99-2923-B-001-001-MY3) from the National Science Council, Taiwan, the Russian Foundation for Basic Research, Grant No. 14-04-01060-a and Siberian Branch of Russian Academy of Sciences, joint Taiwan-Siberian Project No. 11.
Предметные рубрики: SHALLOW COASTAL WATER
RIBOSOMAL-RNA GENE
EMENDED DESCRIPTION
PSEUDIDIOMARINA
PHYLOTYPES
SEQUENCE
TAIWAN
Аннотация: Strain AIS(T), an aerobic halophilic, Gram-reaction-negative, heterotrophic bacterium isolated from the water of Shira Lake in Khakasia, southern Siberia, was characterized using a polyphasic approach. Our analysis of the 16S rRNA gene sequences showed that 'Aliidiomarina haloalkalitolerans', 'Allidiomarina sanyensis', Idiomarina maris and AIS(T) formed a distinct lineage. The sequence similarities between AIS(T) and the type strains of species of the genera Idiomarina and Aliidiomarina were 91.6-95.1 % and 94.0-96.9 %, respectively. The major isoprenoid quinone of AIS(T) was ubiquinone 8 (Q-8). Predominant cellular fatty acids were iso-C-17:0, iso-C-15:0 and summed feature 9. The genomic DNA G+C content was 45.8 mol%. It is concluded that AIS(T) represents a novel species of the genus Aliidiomarina, and the name Aliidiomarina shirensis sp. nov. is herein proposed for it. The type strain is AIST (=JCM 17761(T)=BCRC 80327(T)). Based on its fatty acid profile and our phylogenetic analysis, we propose that Idiomarina mans be transferred to the genus Aliidiomarina.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T..., Kiselev E..., Vinogradova O..., Nikolaeva E..., Chistyakov A..., Sukovatiy A..., Shishatskaya E...
Заглавие : A Glucose-Utilizing Strain, Cupriavidus euthrophus B-10646: Growth Kinetics, Characterization and Synthesis of Multicomponent PHAs
Колич.характеристики :15 с
Коллективы : Project "Biotechnologies of novel biomaterials: Innovative Biopolymers and Biomedicine Devices" [11.G34.31.0013]
Место публикации : PLoS One: PUBLIC LIBRARY SCIENCE, 2014. - Vol. 9, Is. 2. - Ст.e87551. - ISSN 1932-6203, DOI 10.1371/journal.pone.0087551
Примечания : Cited References: 64. - This study was financially supported by Project "Biotechnologies of novel biomaterials: Innovative Biopolymers and Biomedicine Devices" (Agreement No. 11.G34.31.0013 with Amendment No. 1 of 15 February 2013) in accordance with Resolution No. 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning." The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Предметные рубрики: RALSTONIA-EUTROPHA
BIODEGRADABLE POLYHYDROXYALKANOATES
AEROMONAS-HYDROPHILA
ESCHERICHIA-COLI
MOLECULAR-WEIGHT
SURFACE-ENERGY
NORTH PACIFIC
TERPOLYESTER
BIOSYNTHESIS
POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE-CO-3-HYDROXYHEXANOATE)
Аннотация: This study investigates kinetic and production parameters of a glucose-utilizing bacterial strain, C. eutrophus B-10646, and its ability to synthesize PHA terpolymers. Optimization of a number of parameters of bacterial culture (cell concentration in the inoculum, physiological activity of the inoculum, determined by the initial intracellular polymer content, and glucose concentration in the culture medium during cultivation) provided cell concentrations and PHA yields reaching 110 g/L and 80%, respectively, under two-stage batch culture conditions. Addition of precursor substrates (valerate, hexanoate, propionate, c-butyrolactone) to the culture medium enabled synthesis of PHA terpolymers, P(3HB/3HV/4HB) and P(3HB/3HV/3HHx), with different composition and different molar fractions of 3HB, 3HV, 4HB, and 3HHx. Different types of PHA terpolymers synthesized by C. eutrophus B-10646 were used to prepare films, whose physicochemical and physicalmechanical properties were investigated. The properties of PHA terpolymers were significantly different from those of the P3HB homopolymer: they had much lower degrees of crystallinity and lower melting points and thermal decomposition temperatures, with the difference between these temperatures remaining practically unchanged. Films prepared from all PHA terpolymers had higher mechanical strength and elasticity than P3HB films. In spite of dissimilar surface structures, all films prepared from PHA terpolymers facilitated attachment and proliferation of mouse fibroblast NIH 3T3 cells more effectively than polystyrene and the highly crystalline P3HB.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Ketelaars M., Gibson B.G., Lee J.
Заглавие : Interaction of Photobacterium leiognathi and Vibrio fischeri Y1 luciferases with fluorescent (antenna) proteins: Bioluminescence effects of the aliphatic additive
Место публикации : Biochemistry. - 1996. - Vol. 35, Is. 37. - С. 12086-12093. - ISSN 00062960 (ISSN) , DOI 10.1021/bi9608931
Ключевые слова (''Своб.индексиров.''): luciferase--anisotropy--antenna--article--bioluminescence--complex formation--energy transfer--enzyme active site--enzyme kinetics--nonhuman--priority journal--protein protein interaction--spectroscopy--vibrionaceae--bacterial proteins--carrier proteins--cloning, molecular--dithionite--flavin mononucleotide--kinetics--luciferases--luminescent measurements--luminescent proteins--models, structural--photobacterium--protein binding--protein conformation--recombinant proteins--spectrophotometry--vibrio--bacteria (microorganisms)--photobacterium--photobacterium leiognathi--vibrio fischeri--vibrionaceae
Аннотация: The kinetics of the bacterial bioluminescence reaction is altered in the presence of the fluorescent (antenna) proteins, lumazine protein (LumP) from Photobacterium or the yellow fluorescence proteins (YFP) having FMN or Rf bound, from Vibrio fischeri strain Y1. Depending on reaction conditions, the bioluminescence intensity and its decay rate may be either enhanced or strongly quenched in the presence of the fluorescent proteins. These effects can be simply explained on the basis of the same protein-protein complex model that accounts for the bioluminescence spectral shifts induced by these fluorescent proteins. In such a complex, where the fluorophore evidently is in proximity to the luciferase active site, it is expected that the on off rate of certain aliphatic components of the reaction should be altered with a consequent shift in the equilibria among the luciferase intermediates, as recently elaborated in a kinetic scheme. These aliphatic components are the bioluminescence reaction substrate, tetradecanal or other long-chain aldehyde, its carboxylic acid product, or dodecanol used as a stabilizer of the luciferase peroxyflavin. No evidence can be found for the protein- protein interaction in the absence of the aliphatic component.
Scopus
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Ketelaars M..., Gibson B.G., Lee J...
Заглавие : Interaction of Photobacterium leiognathi and Vibrio fischeri Y1 luciferases with fluorescent (Antenna) proteins: Bioluminescence effects of the aliphatic additive
Колич.характеристики :8 с
Место публикации : Biochemistry: AMER CHEMICAL SOC, 1996. - Vol. 35, Is. 37. - С. 12086-12093. - ISSN 0006-2960, DOI 10.1021/bi9608931
Примечания : Cited References: 41
Предметные рубрики: BACTERIAL LUCIFERASE
LUMAZINE PROTEIN
FLAVIN INTERMEDIATE
ANGSTROM RESOLUTION
RIBOFLAVIN PROTEIN
PURIFICATION
MECHANISM
EMISSION
ALDEHYDE
INHIBITION
Аннотация: The kinetics of the bacterial bioluminescence reaction is altered in the presence of the fluorescent (antenna) proteins, lumazine protein (LumP) from Photobacterium or the yellow fluorescence proteins (YFP) having FMN or Rf bound, from Vibrio fischeri strain Y1, Depending on reaction conditions, the bioluminescence intensity and its decay rate may be either enhanced or strongly quenched in the presence of the fluorescent proteins. These effects call be simply explained on the basis of the same protein-protein complex model that accounts for the bioluminescence spectral shifts induced by these fluorescent proteins. In such a complex, when the fluorophore evidently is in proximity to the luciferase active site, it is expected that the on-off rate of certain aliphatic components of the reaction should be altered with a consequent shift in the equilibria among the luciferase intermediates, as recently elaborated in a kinetic scheme, These aliphatic components are the bioluminescence reaction substrate, tetradecanal or other long-chain aldehyde, its carboxylic acid product, or dodecanol used as a stabilizer of the luciferase peroxyflavin. No evidence can be found or the protein-protein interaction in the absence of the aliphatic component.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Gibson B.G., Lee J...
Заглавие : Direct measurement of excitation transfer in the protein complex of bacterial luciferase hydroxyflavin and the associated yellow fluorescence proteins from Vibrio fischeri Y1
Колич.характеристики :6 с
Место публикации : Biochemistry: AMER CHEMICAL SOC, 1996. - Vol. 35, Is. 25. - С. 8413-8418. - ISSN 0006-2960, DOI 10.1021/bi952691v
Примечания : Cited References: 24
Предметные рубрики: LUMAZINE PROTEIN
LUMINOUS BACTERIUM
STRAIN Y-1
BIOLUMINESCENCE
EMISSION
PURIFICATION
TRANSIENT
LIGHT
Аннотация: Time-resolved fluorescence was used to directly measure the energy transfer rate constant in the protein-protein complex involved in the yellow bioluminescence of Vibrio fischeri, strain Y1. In this reaction the putative donor is the fluorescent transient intermediate, luciferase hydroxyflavin, which exhibits a major fluorescence lifetime of the bound flavin of 10 ns. On addition of the acceptor, the V. fischeri yellow fluorescence protein containing either FMN or riboflavin as ligand, a rapid decay time, 0.25 ns, becomes predominant. The same results are observed using rec-luciferase from Photobacterium leiognathi to produce the donor. Because of favorable spectral separation in this system, this rapid decay rate of 4 ns(-1), can be directly equated to the energy transfer rate. This rate is ten times higher than the rate previously observed in the Photobacterium luciferase hydroxyflavin-lumazine protein, donor-acceptor system, derived from emission anisotropy measurements. This ten-times ratio is close to the ratio of spectral overlaps of the donor fluorescence with the acceptor absorption, between these two systems, so it is concluded that the topology of the protein complexes in both cases, must be very similar. Energy transfer is also monitored by the loss of steady-state fluorescence intensity at 460 nm of the donor, on addition of the acceptor protein. A fluorescence titration indicates that luciferase hydroxyflavin and the yellow protein complex with a 1:1 stoichiometry with a K-d of 0.7 mu M (0 degrees C). These parameters account for the bioluminescence spectral shifting effects observed in these reactions.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Popova L.Y., Kargatova T.V., Maksimova E.E., Belyavskaya V.A.
Заглавие : A study on the possibility of environmental adaptation of a Bacillus subtilis strain containing a recombinant plasmid with the gene of human interferon alpha 2
Колич.характеристики :5 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1997. - Vol. 66, Is. 6. - P637-641. - ISSN 0026-2617
Примечания : Cited References: 13
Предметные рубрики: GENETICALLY-MODIFIED MICROORGANISMS
Ключевые слова (''Своб.индексиров.''): microecosystem--bacillus subtilis--recombinant plasmid--interferon--adaptation
Аннотация: Adaptation of the Bacillus subtilis strain 2335/105 (Km(r)Inf(+)) containing a recombinant plasmid encoding the extracellular human interferon alpha 2 was studied under various conditions. Stability of the plasmid in the population of B. subtilis 2335/105 was estimated under nonselective conditions. The plasmid-free cells and cells with a low number of plasmid copies were found to accumulate progressively, constituting 80% of the population after 10 culture passages, indicating the poor competitiveness of cells carrying a high number of plasmid copies. The behavior of vegetative cells of the recombinant strain introduced into aquatic microcosms differing in trophic chain length was studied. Within the first 10 days, the lysis of vegetative cells of B. subtilis 2335/105 occurred; the number of viable spores was very low but remained constant for half a year.
WOS
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Maksimova E.E., Popova L.Y., Kargatova T.V., Shpagina V.V.
Заглавие : Controlled expression of bacterial luminescence genes cloned in a multicopy recombinant plasmid
Колич.характеристики :4 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1997. - Vol. 66, Is. 2. - P184-187. - ISSN 0026-2617
Примечания : Cited References: 17
Предметные рубрики: GENETICALLY-MODIFIED MICROORGANISMS
BIOLUMINESCENCE
ENVIRONMENT
Ключевые слова (''Своб.индексиров.''): recombinant plasmid--escherichia coli--luminescence--catabolite repression
Аннотация: Luminescence and growth responses of the recombinant strain Escherichia coil Z905 (Ap(r)Lux(+)) to different concentrations of ampicillin depended on the source of carbon and energy. When glycerol was used as the substrate, the intensity of luminescence rose with the ampicillin concentration in the medium. Glucose caused catabolite repression of cell luminescence up to the late stationary phase, and ampicillin enhanced this effect. The feasibility of controlling the expression of cloned lux genes was shown.
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