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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Gibson B.G., Lee J.
Заглавие : The yellow bioluminescence bacterium, Vibrio fischeri Y1, contains a bioluminescence active riboflavin protein in addition to the yellow fluorescence FMN protein
Место публикации : Biochemical and Biophysical Research Communications. - 1995. - Vol. 211, Is. 3. - С. 774-779. - ISSN 0006291X (ISSN) , DOI 10.1006/bbrc.1995.1880
Ключевые слова (''Своб.индексиров.''): riboflavin--article--bioluminescence--fluorescence--nonhuman--priority journal--protein analysis--protein synthesis--vibrio--vibrionaceae--bacterial proteins--chromatography, gel--chromatography, thin layer--flavin mononucleotide--flavoproteins--luminescence--riboflavin--spectrometry, fluorescence--support, u.s. gov't, p.h.s.--vibrio--bacteria (microorganisms)--photobacterium--vibrio--vibrio fischeri
Аннотация: The yellow bioluminescence Y1 strain of Vibrio fischeri can produce a 22 kDa protein with either FMN or riboflavin as a bound fluorophore. Both forms are active for shifting the bioluminescence spectral maximum. The fluorescence spectral distribution of the two proteins differs slightly and the in vivo emission appears to be an equal mixture of the two. The bioluminescence activity of the riboflavin Y1 protein contrasts with the inactivity of the related Photobacterium type.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tirranen L.S., Gitelson I.I.
Заглавие : The role of volatile metabolites in microbial communities of the LSS higher plant link
Место публикации : Advances in Space Research. - 2006. - Vol. 38, Is. 6. - С. 1227-1232. - ISSN 02731177 (ISSN) , DOI 10.1016/j.asr.2006.02.038
Ключевые слова (''Своб.индексиров.''): inhibiting, bactericidal, stimulating effect--microbial interaction--type of interaction--volatile and non-volatile metabolites--growth kinetics--microbiology--microorganisms--plants (botany)--sensitivity analysis--toxic materials--inhibiting, bactericidal, stimulating effect--microbial interaction--type of interaction--volatile and non-volatile metabolites--metabolites
Аннотация: The paper addresses the possibility of controlling the microbial community composition through metabolites produced by microbes. The comparative analysis of experimental data has shown that volatile metabolites make a much greater contribution to the microbial interactions than nonvolatile ones. It has been found that interaction of microorganisms via the volatiles they release occurs frequently and is typical of a number of microorganisms. Volatile metabolites released by microorganisms produce an inhibitory, sometimes bactericidal, effect on the vital functions of bacteria. The stimulating action occurs 6-8 times less frequently. The range of action on the growth of the test cultures and the range of sensitivity to the effect of volatile metabolites of the study microbes have been found to be individual. Comparative cluster analysis of the ranges of action of 100 study cultures has shown that in the investigated set of microorganism species interaction was performed via a set of volatiles of an inhibiting action (82) and of a stimulating action (52). It has been found that release of volatile metabolites by the studied microorganisms depends upon the culture age, concentrations of components of the nutrient medium, and volatile by-products released by other microorganisms. This production can be increased or decreased by the action of volatile metabolites of other microbes. This is related to strain features and culture age. The prospects of using these regulating metabolites depend on the "range", specificity and safety for other members of the microbial community in insufficient concentrations. Volatiles produced by plants and microorganisms as well as by other components of the system - humans and processing equipment installed inside the closed ecosystem - could influence the formation not only of the microbial community but also of the gas composition of the system's atmosphere, through which they could affect the state of the plants. Specially performed experiments have shown that volatile metabolites of microorganisms can accumulate in their habitat, dissolve in the atmospheric water, and maintain their biological activity for days. It has been determined that volatile metabolites of some microorganisms are toxic for plants. Growth of seedling roots is inhibited more than growth of stems. В© 2006 COSPAR.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mogil'naya O.A., Krylova T.Y., Popova L.Y.
Заглавие : The morphological characteristics and the dynamics of biofilms formed by a transgenic Bacillus subtilis strain
Колич.характеристики :2 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 2003. - Vol. 72, Is. 4. - P509-510. - ISSN 0026-2617, DOI 10.1023/A:1025065311507
Примечания : Cited References: 5
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Altukhova O.V.
Заглавие : The autotrophic synthesis of polyhydroxyalkanoate by Alcaligenes eutrophus in the presence of carbon monoxide
Место публикации : Microbiology. - 2001. - Vol. 70, Is. 6. - С. 640-646. - ISSN 00262617 (ISSN)
Ключевые слова (''Своб.индексиров.''): co inhibition--hydrogen-oxidizing bacteria--polyhydroxyalkanoates (phas)--synthesis
Аннотация: The CO-resistant strain B5786 of the hydrogen-oxidizing bacterium Alcaligenes eutrophus was found to be able to synthesize polyhydroxyalkanoates (PHAs) under the conditions of growth limitation by nitrogen deficiency (the factor that promotes PHA synthesis) and growth inhibition by carbon monoxide. The gas mixtures that contained from 5 to 20 vol % CO did not inhibit the key enzymes of PHA synthesis-?-ketothiolase, acetoacetyl-CoA reductase, hydroxybutyrate dehydrogenase, and PHA synthase. In the presence of CO, cells accumulated up to 70-75 wt % PHA (with respect to the dry biomass) without any noticeable increase in the consumption of the gas substrate. Chromatographic-mass spectrometric analysis showed that the PHA synthesized by A. eutrophus is a copolymer containing more than 99 mol % ?-hydroxybutyrate and trace amounts of ?-hydroxyvalerate. The PHA synthesized under the conditions described did not differ from that synthesized by A. eutrophus cells from electrolytic hydrogen. В© 2001 MAIK "Nauka/Interperiodica".
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Altukhova O.V.
Заглавие : The autotrophic synthesis of polyhydroxyalkanoate by alcaligenes eutrophus in the presence of carbon monoxide
Место публикации : Mikrobiologiya. - 2001. - Vol. 70, Is. 6. - С. 745-752. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): co inhibition--hydrogen-oxidizing bacteria--polyhydroxyalkanoates (phas)--synthesis--alkane--carbon monoxide--fatty acid--hydroxyacid--lipid--nitrogen--polyester--article--chemistry--culture medium--drug antagonism--growth, development and aging--mass spectrometry--metabolism--wautersia eutropha--alkanes--carbon monoxide--culture media--cupriavidus necator--fatty acids--hydroxy acids--lipids--mass spectrometry--nitrogen--polyesters
Аннотация: The CO-resistant strain B5786 of the hydrogen-oxidizing bacterium Alcaligenes eutrophus was found to be able to synthesize polyhydroxyalkanoates (PHAs) under the conditions of growth limitation by nitrogen deficiency (the factor that promotes PHA synthesis) and growth inhibition by carbon monoxide. The gas mixtures that contained from 5 to 20 vol % CO did not inhibit the key enzymes of PHA synthesis-?-ketothiolase, acetoacetyl-CoA reductase, hydroxybutyrate dehydrogenase, and PHA synthase. In the presence of CO, cells accumulated up to 70-75 wt % PHA (with respect to the dry biomass) without any noticeable increase in the consumption of the gas substrate. Chromatographic-mass spectrometric analysis showed that the PHA synthesized by A. eutrophus is a copolymer containing more than 99 mol % ?-hydroxybutyrate and trace amounts of ?-hydroxyvalerate. The PHA synthesized under the conditions described did not differ from that synthesized by A. eutrophus cells from electrolytic hydrogen.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Altukhova O.V.
Заглавие : The autotrophic synthesis of polyhydroxyalkanoate by Alcaligenes eutrophus in the presence of carbon monoxide
Колич.характеристики :7 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 2001. - Vol. 70, Is. 6. - P640-646. - ISSN 0026-2617, DOI 10.1023/A:1013175413013
Примечания : Cited References: 19
Ключевые слова (''Своб.индексиров.''): hydrogen-oxidizing bacteria--polyhydroxyalkanoates (phas)--synthesis--co inhibition
Аннотация: The CO-resistant strain B5786 of the hydrogen-oxidizing bacterium Alcaligenes eutrophus was found to be able to synthesize polyhydroxyalkanoates (PHAs) under the conditions of growth limitation by nitrogen deficiency (the factor that promotes PHA synthesis) and growth inhibition by carbon monoxide. The gas mixtures that contained from 5 to 20 vol % CO did not inhibit the key enzymes of PHA synthesis-beta-ketothiolase. acetoacetyl-CoA reductase, hydroxybutyrate dehydrogenase, and PHA synthase. In the presence of CO, cells accumulated up to 70-75 wt % PHA (with respect to the dry biomass) without any noticeable increase in the consumption of the gas substrate. Chromatographic-mass spectrometric analysis showed that the PHA synthesized by A. eutrophus is a copolymer containing more than 99 mol % beta-hydroxybutyrate and trace amounts of beta-hydroxyvalerate. The PHA synthesized under the conditions described did not differ from that synthesized by A. eutrophus cells from electrolytic hydrogen.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T., Kiselev E., Zhila N., Shishatskaya E.
Заглавие : Synthesis of Polyhydroxyalkanoates by Hydrogen-Oxidizing Bacteria in a Pilot Production Process
Место публикации : Biomacromolecules: American Chemical Society, 2019. - Article in press. - ISSN 15257797 (ISSN) , DOI 10.1021/acs.biomac.9b00295
Аннотация: The synthesis of polyhydroxyalkanoates (PHAs) was scaled up to pilot production in a 150-L fermenter on sugars (fructose and glucose) and purified and crude glycerol in a culture of the wild-type strain Cupriavidus necator B-10646. Over 60 h of cultivation, a cell concentration of 150-160 g/L was obtained on purified glycerol and glucose; cultivation on fructose and crude glycerol resulted in a cell concentration of 130 ± 10 g/L. Polymer content and yield coefficients for the biomass were similar on all substrates (80-85 wt % and 0.29-0.33 kg biomass/kg carbon substrate, respectively). Copolymers poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and poly(3-hydroxybutyrate-co-4-hydroxybutyrate) and terpolymers poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxybutyrate) having a decreased degree of crystallinity (36-46%) were first synthesized in the scaled-up process using C. necator B-10646 cultivated on glycerol. These results will provide the basis for scaling-up PHA synthesis in an organotrophic C. necator B-10646 culture. © 2019 American Chemical Society.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T. G., Syrvacheva D. A., Zhila N. O., Sukovatiy A. G.
Заглавие : Synthesis of P(3HB-co-3HHx) copolymers containing high molar fraction of 3-hydroxyhexanoate monomer by Cupriavidus eutrophus B10646
Место публикации : J. Chem. Technol. Biotechnol. - 2016. - Vol. 91, Is. 2. - С. 416-425. - ISSN 02682575 (ISSN) , DOI 10.1002/jctb.4592
Ключевые слова (''Своб.индексиров.''): growth kinetics--physicochemical and mechanical properties--poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)--wild-type strain cupriavidus eutrophus b10646--biomaterials--biomechanics--chemical industry--cultivation--growth kinetics--mechanical properties--organic compounds--polymers--sodium--3-hydroxyhexanoate--bacterial strains--cultivation conditions--kinetic properties--physico-chemical and mechanical properties--physiological range--poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)--wild-type strain--strain
Аннотация: BACKGROUND: P(3HB-co-3HHx) copolymers are very promising biomaterials. The main challenge in the production of these polymers is to simultaneously achieve high cell biomass; high P(3HB-co-3HHx) content; and high molar fraction of 3HHx in P(3HB-co-3HHx). The most common approach to production of these copolymers is the use of recombinant bacterial strains. The purpose of this study was to optimize the process of production of P(3HB-co-3HHx) copolymers containing high molar fractions of 3HHx by using the wild-type strain Cupriavidus eutrophus B10646. RESULTS: Kinetic properties of C. eutrophus B10646 were studied during cultivation of the cells on substrates necessary for P(3HB-co-3HHx) synthesis: glucose, nitrogen, sodium hexanoate, and sodium acrylate. The physiological ranges of their effects were determined experimentally, and C. eutrophus B10646 was grown in culture media with different dosages of these substrates. P(3HB-co-3HHx) copolymers with different molar fractions of 3HHx, including high ones (12 to 68%), were synthesized, and their physicochemical and mechanical properties were investigated. CONCLUSION: For the first time, cultivation conditions of Cupriavidus eutrophus B10646 enabled production of high biomass yields (5-6gL-1) and high content of the polymer (60-75%) that contained high 3HHx molar fraction. By varying the 3HB/3HHx ratio, one can change physicochemical and mechanical properties of P(3HB-co-3HHx) copolymers. © 2014 Society of Chemical Industry © 2016 Society of Chemical Industry.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Lobova T.I., Popova L.Yu., Pechurkin N.S.
Заглавие : Survival and alteration of the plasmid-containing microorganism Escherichia coli Z905/pPHL7 introduced into manmade closed aquatic microcosms
Место публикации : Advances in Space Research. - 2003. - Vol. 31, Is. 7. - С. 1763-1768. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(03)00118-2
Ключевые слова (''Своб.индексиров.''): cells--escherichia coli--salts--recombinant plamid--space research--ampicillin--chloride--inorganic salt--magnesium--mineral--potassium--sodium--sulfate--genetically modified organism--article--bacterial count--chemoluminescence--culture medium--drug effect--escherichia coli--genetics--growth, development and aging--microbiology--microclimate--penicillin resistance--plasmid--russian federation--transgenic organism--ampicillin--ampicillin resistance--chemiluminescent measurements--chlorides--colony count, microbial--culture media--ecological systems, closed--escherichia coli--magnesium--minerals--organisms, genetically modified--plasmids--potassium--russia--salts--sodium--sulfates--water microbiology
Аннотация: It has been demonstrated that the transgenic microorganism Escherichia coli Z905/pPHL7 (Ap'Lux+) can exist for a long time at an elevated concentration of mineral salts. The microorganism was introduced into microcosms with sterile brackish water (salinity variable from 21 to 22 g 1-1) taken from Lake Shira (Khakasia, Russia). The survivof the microorganism was estimated both by measuring the growth of the colonies on solid nutrient media and by the bioluminescence exhibited by the transgenic strain in samples from the microcosms and in the enrichment culture with the added selective factor - ampicillin (50 ?g/ml). In the enrichment culture, the bioluminescent signal was registered through the 160-day experiment. It has been shown that in the closed microcosms with brackish water the E. coli strain becomes heterogeneous in its ampicillin resistance. The populations of the transgenic strain were mainly represented by isolates able to persist in the medium containing 50 ?g/ml, but there were also the cells (about 10%) with the threshold of ampicillin resistance not more than 0.05 ?g/ml. Thus, it was shown that in the microcosms with brackish water and in the absence of the selective factor the transgenic strain survives and retains the recombinant plasmid. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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10.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Boyandin A.N., Lobova T.I., Popova L.Y., Pechurkin PNS
Заглавие : Survival and alteration of the plasmid-containing microorganism Escherichia coli Z905/pPHL7 introduced into manmade closed aquatic microcosms
Колич.характеристики :6 с
Место публикации : SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - P1763-1768. - ISBN 0273-1177, DOI 10.1016/S0273-1177(03)00118-2
Примечания : Cited References: 9
Предметные рубрики: MARKER
SOIL
Аннотация: It has been demonstrated that the transgenic microorganism Escherichia coli Z905/pPHL7 (Ap(r)Lux(+)) can exist for a long time at an elevated concentration of mineral salts. The microorganism was introduced into microcosms with sterile brackish water (salinity variable from 21 to 22 g l(-1)) taken from Lake Shira (Khakasia, Russia). The survival of the microorganism was estimated both by measuring the growth of the colonies on solid nutrient media and by the bioluminescence exhibited by the transgenic strain in samples from the microcosms and in the enrichment culture with the added selective factor - ampicillin (50 mug/ml). In the enrichment culture, the bioluminescent signal was registered through the 160-day experiment. It has been shown that in the closed microcosms with brackish water the E. coli strain becomes heterogeneous in its ampicillin resistance. The wpopulations of the transgenic strain were mainly represented by isolates able to persist in the medium containing 50 mug/ml, but there were also the cells (about 10%) with the threshold of ampicillin resistance not more than 0.05 mug/ml. Thus, it was shown that in the microcosms with brackish water and in the absence of the selective factor the transgenic strain survives and retains the recombinant plasmid. (C) 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Vasiliev A.D., Zeer E.P., Petrakovskaya E.A., Falaleev O.V.
Заглавие : Study of the molecular structure of polyhydroxybutyrate, a termoplastic and degradable biopolymer
Место публикации : Biofizika: MEZHDUNARODNAYA KNIGA, 2000. - Vol. 45, Is. 3. - С. 445-451. - 7. - ISSN 0006-3029
Примечания : Cited References: 16
Предметные рубрики: POLY(BETA-HYDROXYBUTYRATE)
BIOTECHNOLOGY
Ключевые слова (''Своб.индексиров.''): polyhydroxybutyrate--crystalline and amorphous phases--molecular structure
Аннотация: The molecular structure of polyhydroxybutyrate from hydrogen-oxidizing bacteria Alcaligenes eutrophus was studied by X-ray diffraction analysis. It was shown that the degree of crystallinity of various samples depends little on the conditions of their preparation and is equal to 0.62-0.76. The molecular structure of solid samples and solution of polyhydroxybutyrate in chloroform were studied by the NMR and EPR methods. The conclusion is made that the molecular structure of polyhydroxybutyrate does not depend on the features of the strain and conditions of carbon nutrition of microorganisms producing polyhydroxybutyrate. Defects induced by gamma -radiation in polyhydroxybutyrate were studied. Free radicals were isolated, and their structure was decoded.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Voinov N.A.
Заглавие : Study of Ralstonia eutropha culture producing polyhydroxyalkanoates on products of coal processing
Место публикации : Prikladnaia biokhimiia i mikrobiologiia. - 2004. - Vol. 40, Is. 3. - С. 296-300. - ISSN 05551099 (ISSN)
Ключевые слова (''Своб.индексиров.''): carbon monoxide--coal--polyester--antibiotic resistance--article--culture medium--enzyme specificity--gas--growth, development and aging--metabolism--microbiology--wautersia eutropha--carbon monoxide--coal--culture media--cupriavidus necator--drug resistance, microbial--gases--polyesters--substrate specificity
Аннотация: Kinetic indices of growth, polyhydroxyalkanoate (PHA) accumulation, and gas exchange have been studied in a culture of the carbon monoxide-resistant hydrogen strain Ralstonia eutropha B-5786 grown on a gaseous substrate (GS) obtained by lignite gasification. The GS was shown to be suitable for PHA production. To increase the degree of GS consumption, various modes of gas supply to the culture were tested. Based on the results, an algorithm was developed for calculating and controlling gas-exchange parameters in the PHA-accumulating culture of Ralstonia eutropha, grown on a new GS allowing high polymer yields (up to 75%) and degrees of the substrate utilization (up to 90%).
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Vasil'ev A.D., Zeer E.P., Petrakovskaia E.A., Falaleev O.V.
Заглавие : Study of molecular structure of polyhydroxybutyrate-a termoplastic anddegradable biopolymer
Место публикации : Biofizika. - 2000. - Vol. 45, Is. 3. - С. 445-451. - ISSN 00063029 (ISSN)
Ключевые слова (''Своб.индексиров.''): carbon--hydroxybutyric acid--polyester--alcaligenes--article--chemistry--crystallization--alcaligenes--carbon--crystallization--hydroxybutyrates--polyesters
Аннотация: The molecular structure of polyhydroxybutyrate from hydrogen-oxidizing bacteria Alcaligenes eutrophus was studied by X-ray diffraction analysis. It was shown that the degree of crystallinity of various samples depends little on the conditions of their preparation and is equal to 0.62-0.76. The molecular structure of solid samples and solution of polyhydroxybutyrate in chloroform were studied by the NMR and EPR methods. The conclusion is made that the molecular structure of polyhydroxybutyrate does not depend on the features of the strain and conditions of carbon nutrition of microorganisms producing polyhydroxybutyrate. Defects induced by gamma-radiation in polyhydroxybutyrate were studied. Free radicals were isolated, and their structure was decoded.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Voinov N.A.
Заглавие : Study of a Ralstonia eutropha culture producing polyhydroxyalkanoates on products of coal processing
Колич.характеристики :4 с
Место публикации : Appl. Biochem. Microbiol.: MAIK NAUKA/INTERPERIODICA, 2004. - Vol. 40, Is. 3. - P249-252. - ISSN 0003-6838, DOI 10.1023/B:ABIM.0000025946.47013.03
Примечания : Cited References: 18
Аннотация: Kinetic indices of growth, polyhydroxyalkanoate (PHA) accumulation, and gas exchange were studied in a culture of the carbon monoxide-resistant hydrogen strain Ralstonia eutropha B-5786 grown on a gaseous substrate (GS) obtained by lignite gasification. The GS was shown to be suitable for PHA production. To increase the degree of GS consumption, various modes of gas supply to the culture were tested. Based on the results, an algorithm was developed for calculating and controlling gas-exchange parameters in the PHA-accumulating culture of Ralstonia eutropha, grown on a new GS allowing high polymer yields (up to 75%) and degrees of substrate utilization (up to 90%).
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Voinov N.A.
Заглавие : Study of a Ralstonia eutropha culture producing polyhydroxyalkanoates on products of coal processing
Место публикации : Applied Biochemistry and Microbiology. - 2004. - Vol. 40, Is. 3. - С. 249-252. - ISSN 00036838 (ISSN) , DOI 10.1023/B:ABIM.0000025946.47013.03
Ключевые слова (''Своб.индексиров.''): alkanoic acid--carbon monoxide--coal--hydrogen--lignite--algorithm--article--bacterial strain--bacterium culture--coal gasification--controlled study--gas--gas exchange--mathematical computing--nonhuman--ralstonia eutropha--bacteria (microorganisms)--ralstonia--wautersia eutropha
Аннотация: Kinetic indices of growth, polyhydroxyalkanoate (PHA) accumulation, and gas exchange were studied in a culture of the carbon monoxide-resistant hydrogen strain Ralstonia eutropha B-5786 grown on a gaseous substrate (GS) obtained by lignite gasification. The GS was shown to be suitable for PHA production. To increase the degree of GS consumption, various modes of gas supply to the culture were tested. Based on the results, an algorithm was developed for calculating and controlling gas-exchange parameters in the PHA-accumulating culture of Ralstonia eutropha, grown on a new GS allowing high polymer yields (up to 75%) and degrees of substrate utilization (up to 90%).
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Zhila N.O.
Заглавие : Specificity of lipid composition in two Botryococcus strains, the producers of liquid hydrocarbons
Колич.характеристики :7 с
Место публикации : Russ. J. Plant Physiol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2003. - Vol. 50, Is. 5. - P627-633. - ISSN 1021-4437, DOI 10.1023/A:1025636007023
Примечания : Cited References: 17
Предметные рубрики: GREEN-ALGA BOTRYOCOCCUS
BRAUNII
CULTURES
BATCH
RACE
Ключевые слова (''Своб.индексиров.''): botryococcus--hydrocarbons--structure--mass spectra
Аннотация: The structure of liquid hydrocarbons and fatty acids produced by the green alga Botryococcus was identified. Two representatives of this alga, Botryococcus braunii Kutz, strain IPPAS H-252, introduced into culture earlier and an organism isolated for the first time from the Shira Lake, were used for this identification. Fatty acid composition of B. braunii, strain H-252, lipids was characterized by a high content of trienoic acids of C16-C18 series. The hydrocarbon composition of this strain was represented by straight-chain and branched-chain C14-C28 components; long-chain linear aliphatic C20-C27 hydrocarbons (54.4%) and 2,6,10,14-tetramethylhexadecane (20.5%) predominated among them. The strain H-252 differed in its fatty acid and hydrocarbon composition from the strains described earlier as Botryococcus braunii. The fatty acid composition of the Botryococcus isolate was represented mainly by C12-C32 saturated and monoenoic acids. The hydrocarbons formed by this isolate were represented by dienoic and trienoic components. C29 (48.9-56.3%) and C31 (11.1-16.3%) hydrocarbons predominated among the C23-C31 dienoic hydrocarbons, and C27, C29, and C31 trienoic hydrocarbons comprised 2.5-2.6% of total hydrocarbons. This type of hydrocarbons and the lipid fatty acid composition were characteristic for the race A of B. braunii.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Zhila N.O.
Заглавие : Specificity of Lipid Composition in Two Botryococcus Strains, the Producers of Liquid Hydrocarbons
Место публикации : Russian Journal of Plant Physiology. - 2003. - Vol. 50, Is. 5. - С. 627-633. - ISSN 10214437 (ISSN) , DOI 10.1023/A:1025636007023
Ключевые слова (''Своб.индексиров.''): botryococcus--hydrocarbons--mass spectra--structure--algae--botryococcus--botryococcus braunii--chlorophyta--eukaryota
Аннотация: The structure of liquid hydrocarbons and fatty acids produced by the green alga Botryococcus was identified. Two representatives of this alga, Botryococcus braunii Kutz, strain IPPAS H-252, introduced into culture earlier and an organism isolated for the first time from the Shira Lake, were used for this identification. Fatty acid composition of B. braunii, strain H-252, lipids was characterized by a high content of trienoic acids of C16-C18 series. The hydrocarbon composition of this strain was represented by straight-chain and branched-chain C14-C28 components; long-chain linear aliphatic C20-C27 hydrocarbons (54.4%) and 2,6,10,14-tetramethylhexadecane (20.5%) predominated among them. The strain H-252 differed in its fatty acid and hydrocarbon composition from the strains described earlier as Botryococcus braunii. The fatty acid composition of the Botryococcus isolate was represented mainly by C12-C32 saturated and monoenoic acids. The hydrocarbons formed by this isolate were represented by dienoic and trienoic components. C29 (48.9-56.3%) and C31 (11.1-16.3%) hydrocarbons predominated among the C23-C31 dienoic hydrocarbons, and C27, C29, and C31 trienoic hydrocarbons comprised 2.5-2.6% of total hydrocarbons. This type of hydrocarbons and the lipid fatty acid composition were characteristic for the race A of B. braunii.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Popova L.Y.
Заглавие : Salt-dependent inhibition of light emitting of the luminescent microorganism Escherichia coli Z9051
Колич.характеристики :5 с
Место публикации : Biofizika: MEZHDUNARODNAYA KNIGA, 2001. - Vol. 46, Is. 2. - P251-255. - ISSN 0006-3029
Примечания : Cited References: 12
Ключевые слова (''Своб.индексиров.''): bacterial luminescence--recombinant plasmid--salt concentration
Аннотация: The influence of some mineral salts on the recombinant strain Escherichia coli Z9051 was investigated. It was shown that the composition (NaCl, Na2SO4, MgCl2 and MgSO4) and concentration (5 and 10%) of the salts substantially affect the expression of genes for the luminescence system of fight-emitting bacteria cloned in the plasmid under the control of the lac-promoter. In some cases, the luminescence level of the microorganism in the presence of salts was similar to the luminescence level under catabolite repression by glucose, the more strong influence of the salts exceeding the effect of catabolite repression. The possibility of adaptation of the genetically modified microorganism to the salinity factor is discussed.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Popova L.Y.
Заглавие : Salt-dependent inhibition of light emitting of the luminescent microorganism Escherichia coli Z9051
Место публикации : Biofizika: MEZHDUNARODNAYA KNIGA, 2001. - Vol. 46, Is. 2. - С. 251-255. - 5. - ISSN 0006-3029
Примечания : Cited References: 12
Ключевые слова (''Своб.индексиров.''): bacterial luminescence--recombinant plasmid--salt concentration
Аннотация: The influence of some mineral salts on the recombinant strain Escherichia coli Z9051 was investigated. It was shown that the composition (NaCl, Na2SO4, MgCl2 and MgSO4) and concentration (5 and 10%) of the salts substantially affect the expression of genes for the luminescence system of fight-emitting bacteria cloned in the plasmid under the control of the lac-promoter. In some cases, the luminescence level of the microorganism in the presence of salts was similar to the luminescence level under catabolite repression by glucose, the more strong influence of the salts exceeding the effect of catabolite repression. The possibility of adaptation of the genetically modified microorganism to the salinity factor is discussed.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gusev A.A., Kargatova T.V., Medvedeva S.E., Popova L.Yu.
Заглавие : Quantitative criteria for estimating the effectiveness of bioluminescence expression in natural and transgenic luminescent bacteria
Место публикации : Biophysics. - 2008. - Vol. 53, Is. 5. - С. 417-420. - ISSN 00063509 (ISSN) , DOI 10.1134/S0006350908050175
Ключевые слова (''Своб.индексиров.''): effectiveness of lux-operon expression--natural and transgenic bacteria--bacteria (microorganisms)--escherichia coli--photobacterium leiognathi
Аннотация: Computational coefficients for estimating the effectiveness of bioluminescence expression in natural luminescent bacteria Photobacterium leiognathi 54 and transgenic strain E. coli Z905/pPHL7 bearing lux-operon in a multicopy plasmid are suggested and their use at molecular, cell, and population levels was considered. It was shown that at the population level, all transgenic variants have an advantage over natural variants of P. leiognathi 54 irrespective of the type of lux-operon regulation. At the cell level, the effectiveness of bioluminescence expression in the bright and dim variants of the transgenic strain increased by several orders. At the level of one lux-operon, the effectiveness of expression in the bright variant of the transgenic strain is substantially higher than in the natural bright variant; in dim variants, the efficiency values are similar; the effectiveness of bioluminescence expression in the dark variant of E. coli Z905-2/pPHL7 is by two orders of magnitude lower than in the dark variant of P. leiognathi 54. В© 2008 Pleiades Publishing, Ltd.
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