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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T..., Kiselev E..., Vinogradova O..., Nikolaeva E..., Chistyakov A..., Sukovatiy A..., Shishatskaya E...
Заглавие : A Glucose-Utilizing Strain, Cupriavidus euthrophus B-10646: Growth Kinetics, Characterization and Synthesis of Multicomponent PHAs
Колич.характеристики :15 с
Коллективы : Project "Biotechnologies of novel biomaterials: Innovative Biopolymers and Biomedicine Devices" [11.G34.31.0013]
Место публикации : PLoS One: PUBLIC LIBRARY SCIENCE, 2014. - Vol. 9, Is. 2. - Ст.e87551. - ISSN 1932-6203, DOI 10.1371/journal.pone.0087551
Примечания : Cited References: 64. - This study was financially supported by Project "Biotechnologies of novel biomaterials: Innovative Biopolymers and Biomedicine Devices" (Agreement No. 11.G34.31.0013 with Amendment No. 1 of 15 February 2013) in accordance with Resolution No. 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning." The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Предметные рубрики: RALSTONIA-EUTROPHA
BIODEGRADABLE POLYHYDROXYALKANOATES
AEROMONAS-HYDROPHILA
ESCHERICHIA-COLI
MOLECULAR-WEIGHT
SURFACE-ENERGY
NORTH PACIFIC
TERPOLYESTER
BIOSYNTHESIS
POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE-CO-3-HYDROXYHEXANOATE)
Аннотация: This study investigates kinetic and production parameters of a glucose-utilizing bacterial strain, C. eutrophus B-10646, and its ability to synthesize PHA terpolymers. Optimization of a number of parameters of bacterial culture (cell concentration in the inoculum, physiological activity of the inoculum, determined by the initial intracellular polymer content, and glucose concentration in the culture medium during cultivation) provided cell concentrations and PHA yields reaching 110 g/L and 80%, respectively, under two-stage batch culture conditions. Addition of precursor substrates (valerate, hexanoate, propionate, c-butyrolactone) to the culture medium enabled synthesis of PHA terpolymers, P(3HB/3HV/4HB) and P(3HB/3HV/3HHx), with different composition and different molar fractions of 3HB, 3HV, 4HB, and 3HHx. Different types of PHA terpolymers synthesized by C. eutrophus B-10646 were used to prepare films, whose physicochemical and physicalmechanical properties were investigated. The properties of PHA terpolymers were significantly different from those of the P3HB homopolymer: they had much lower degrees of crystallinity and lower melting points and thermal decomposition temperatures, with the difference between these temperatures remaining practically unchanged. Films prepared from all PHA terpolymers had higher mechanical strength and elasticity than P3HB films. In spite of dissimilar surface structures, all films prepared from PHA terpolymers facilitated attachment and proliferation of mouse fibroblast NIH 3T3 cells more effectively than polystyrene and the highly crystalline P3HB.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T., Kiselev E., Vinogradova O., Nikolaeva E., Chistyakov A., Sukovatiy A., Shishatskaya E.
Заглавие : A glucose-utilizing strain, cupriavidus euthrophus B-10646: Growth kinetics, characterization and synthesis of multicomponent PHAs
Место публикации : PLoS ONE. - 2014. - Vol. 9, Is. 2. - ISSN 19326203 (ISSN) , DOI 10.1371/journal.pone.0087551
Ключевые слова (''Своб.индексиров.''): 3 hydroxybutyrate 3 hydroxyhexanoate 3 hydroxyvalerate copolymer--3 hydroxybutyrate 4 hydroxybutyrate 3 hydroxyvalerate copolymer--copolymer--gamma butyrolactone--glucose--hexanoic acid--poly(3 hydroxybutyric acid)--polyhydroxyalkanoic acid--polystyrene--propionic acid--unclassified drug--valeric acid--animal cell--article--bacterial growth--bacterium culture--cell adhesion--cell proliferation--crystal structure--culture optimization--cupriavidus--cupriavidus euthrophus--decomposition--elasticity--film--glucose utilization--kinetics--mechanics--melting point--mouse--nonhuman--nucleotide sequence--physical chemistry--polymerization--strength--synthesis
Аннотация: This study investigates kinetic and production parameters of a glucose-utilizing bacterial strain, C. eutrophus B-10646, and its ability to synthesize PHA terpolymers. Optimization of a number of parameters of bacterial culture (cell concentration in the inoculum, physiological activity of the inoculum, determined by the initial intracellular polymer content, and glucose concentration in the culture medium during cultivation) provided cell concentrations and PHA yields reaching 110 g/L and 80%, respectively, under two-stage batch culture conditions. Addition of precursor substrates (valerate, hexanoate, propionate, ?-butyrolactone) to the culture medium enabled synthesis of PHA terpolymers, P(3HB/3HV/4HB) and P(3HB/ 3HV/3HHx), with different composition and different molar fractions of 3HB, 3HV, 4HB, and 3HHx. Different types of PHA terpolymers synthesized by C. eutrophus B-10646 were used to prepare films, whose physicochemical and physical-mechanical properties were investigated. The properties of PHA terpolymers were significantly different from those of the P3HB homopolymer: they had much lower degrees of crystallinity and lower melting points and thermal decomposition temperatures, with the difference between these temperatures remaining practically unchanged. Films prepared from all PHA terpolymers had higher mechanical strength and elasticity than P3HB films. In spite of dissimilar surface structures, all films prepared from PHA terpolymers facilitated attachment and proliferation of mouse fibroblast NIH 3T3 cells more effectively than polystyrene and the highly crystalline P3HB. Copyright: © 2014 Volova et al.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Gribovskaya I.V.
Заглавие : A growth medium for the carboxydobacterium Seliberia carboxydohydrogena
Место публикации : Mikrobiologiya. - 1986. - Vol. 55, Is. 1. - С. 72-76. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): carbon monoxide--bacterial growth--culture medium--nonhuman
Аннотация: The growth of the carboxydobacterium Seliberia carboxydohydrogena Z-1062 was studied at a different concentration of mineral elements under the conditions of continuous turbidostat cultivation. The chemical composition of the bacterium was determined and the dynamics of its specific growth rate and the intracellular content of mineral elements were studied when the concentration of these elements was changed in the growth medium. The synthesis of 1 g of biomass by this strain required: N, 120 mg; P, 23 mg; S, 5.5 mg; K, 1.5 mg; Mg, 3.5 mg. Changes in the residual concentrations of the elements within a broad range had no effect on the specific rate of the carboxydobacterial growth and chemical composition.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Popova L.Y., Kargatova T.V., Maksimova E.E., Belyavskaya V.A.
Заглавие : A study on the possibility of environmental adaptation of a Bacillus subtilis strain containing a recombinant plasmid with the gene of human interferon alpha 2
Колич.характеристики :5 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1997. - Vol. 66, Is. 6. - P637-641. - ISSN 0026-2617
Примечания : Cited References: 13
Предметные рубрики: GENETICALLY-MODIFIED MICROORGANISMS
Ключевые слова (''Своб.индексиров.''): microecosystem--bacillus subtilis--recombinant plasmid--interferon--adaptation
Аннотация: Adaptation of the Bacillus subtilis strain 2335/105 (Km(r)Inf(+)) containing a recombinant plasmid encoding the extracellular human interferon alpha 2 was studied under various conditions. Stability of the plasmid in the population of B. subtilis 2335/105 was estimated under nonselective conditions. The plasmid-free cells and cells with a low number of plasmid copies were found to accumulate progressively, constituting 80% of the population after 10 culture passages, indicating the poor competitiveness of cells carrying a high number of plasmid copies. The behavior of vegetative cells of the recombinant strain introduced into aquatic microcosms differing in trophic chain length was studied. Within the first 10 days, the lysis of vegetative cells of B. subtilis 2335/105 occurred; the number of viable spores was very low but remained constant for half a year.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Chiu H.H., Rogozin D.Y., Huang S.P., Degermendzhy A.G., Shieh W.Y., Tang S.L.
Заглавие : Aliidiomarina shirensis sp nov., a halophilic bacterium isolated from Shira Lake in Khakasia, southern Siberia, and a proposal to transfer Idiomarina maris to the genus Aliidiomarina
Колич.характеристики :6 с
Коллективы : National Science Council, Taiwan [NSC 99-2923-B-001-001-MY3]; Russian Foundation for Basic Research [14-04-01060-a]; Siberian Branch of Russian Academy of Sciences, joint Taiwan-Siberian Project [11]
Место публикации : Int. J. Syst. Evol. Microbiol.: SOC GENERAL MICROBIOLOGY, 2014. - Vol. 64. - С. 1334-1339. - ISSN 1466-5026, DOI 10.1099/ijs.0.057851-0. - ISSN 1466-5034
Примечания : Cited References: 22. - We thank Dr Egor S. Zadereev and Dr Vladimir V. Zykov at the Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, for assistance with sampling. This study was supported by Russia Taiwan joint project funding (NSC 99-2923-B-001-001-MY3) from the National Science Council, Taiwan, the Russian Foundation for Basic Research, Grant No. 14-04-01060-a and Siberian Branch of Russian Academy of Sciences, joint Taiwan-Siberian Project No. 11.
Предметные рубрики: SHALLOW COASTAL WATER
RIBOSOMAL-RNA GENE
EMENDED DESCRIPTION
PSEUDIDIOMARINA
PHYLOTYPES
SEQUENCE
TAIWAN
Аннотация: Strain AIS(T), an aerobic halophilic, Gram-reaction-negative, heterotrophic bacterium isolated from the water of Shira Lake in Khakasia, southern Siberia, was characterized using a polyphasic approach. Our analysis of the 16S rRNA gene sequences showed that 'Aliidiomarina haloalkalitolerans', 'Allidiomarina sanyensis', Idiomarina maris and AIS(T) formed a distinct lineage. The sequence similarities between AIS(T) and the type strains of species of the genera Idiomarina and Aliidiomarina were 91.6-95.1 % and 94.0-96.9 %, respectively. The major isoprenoid quinone of AIS(T) was ubiquinone 8 (Q-8). Predominant cellular fatty acids were iso-C-17:0, iso-C-15:0 and summed feature 9. The genomic DNA G+C content was 45.8 mol%. It is concluded that AIS(T) represents a novel species of the genus Aliidiomarina, and the name Aliidiomarina shirensis sp. nov. is herein proposed for it. The type strain is AIST (=JCM 17761(T)=BCRC 80327(T)). Based on its fatty acid profile and our phylogenetic analysis, we propose that Idiomarina mans be transferred to the genus Aliidiomarina.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Chiu H.-H., Rogozin D.Y., Huang S.-P., Degermendzhy A.G., Shieh W.Y., Tang S.-L.
Заглавие : Aliidiomarina shirensis sp. nov., a halophilic bacterium isolated from Shira Lake in Khakasia, southern Siberia, and a proposal to transfer Idiomarina maris to the genus Aliidiomarina
Место публикации : Int. J. Syst. Evol. Microbiol. - 2014. - Vol. 64, Is. PART 4. - Ст.057851. - С. 1334-1339. - ISSN 14665026 (ISSN) , DOI 10.1099/ijs.0.057851-0
Аннотация: Strain AIST, an aerobic halophilic, Gram-reaction-negative, heterotrophic bacterium isolated from the water of Shira Lake in Khakasia, southern Siberia, was characterized using a polyphasic approach. Our analysis of the 16S rRNA gene sequences showed that 'Aliidiomarina haloalkalitolerans', 'Aliidiomarina sanyensis', Idiomarina maris and AIST formed a distinct lineage. The sequence similarities between AIST and the type strains of species of the genera Idiomarina and Aliidiomarina were 91.6-95.1 % and 94.0-96.9 %, respectively. The major isoprenoid quinone of AIST was ubiquinone 8 (Q-8). Predominant cellular fatty acids were iso-C17: 0, iso-C15: 0 and summed feature 9. The genomic DNA G+C content was 45.8 mol%. It is concluded that AIST represents a novel species of the genus Aliidiomarina, and the name Aliidiomarina shirensis sp. nov. is herein proposed for it. The type strain is AIST (= JCM 17761T = BCRC 80327T). Based on its fatty acid profile and our phylogenetic analysis, we propose that Idiomarina maris be transferred to the genus Aliidiomarina. © 2014 IUMS.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kuznetsov A.M., Rodicheva E.K., Medvedeva S.E.
Заглавие : Analysis of river water by bioluminescent biotests
Место публикации : Luminescence. - 1999. - Vol. 14, Is. 5. - С. 263-265. - ISSN 15227235 (ISSN)
Ключевые слова (''Своб.индексиров.''): bioluminescent bioassay--pollution--water quality--fresh water--article--bioassay--escherichia coli--freeze drying--genetic procedures--luminescence--methodology--photobacterium--russian federation--sensitivity and specificity--water pollutant--biological assay--biosensing techniques--escherichia coli--freeze drying--fresh water--luminescence--photobacterium--russia--sensitivity and specificity--water pollutants, chemical
Аннотация: The bacterial bioluminescence has high sensitivity to the action of various inhibitors of biological activity. The lyophilized luminous bacteria Photobacterium phosphoreum (Microbiosensor B17 677F) and luminous strain Escherichia coli (Microbiosensor EC) from the Culture Collection IBSO were used to create bioluminescent biotests. They have been applied in ecological monitoring to determine the overall toxicity of the Yenisei and Angara Rivers and some water sources of Altai Territory. As a rule the heaviest pollution of water in studied rivers was registered near cities and settlements. The luminous bacteria biotests are simple and convenient in work, standardized and quantitative, have rapid response to actions of different substances and high sensitivity to environmental pollutants. It takes less than 30 min to do the biotest (the other biotests take 48-96 h). Copyright В© 1999 John Wiley & Sons, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Lunina O.N., Bryantseva I.A., Akimov V.N., Rusanov I.I., Barinova E.S., Lysenko A.M., Rogozin D.Yu., Pimenov N.V.
Заглавие : Anoxygenic phototrophic bacterial community of Lake Shira (Khakassia)
Место публикации : Microbiology. - 2007. - Vol. 76, Is. 4. - С. 469-479. - ISSN 00262617 (ISSN) , DOI 10.1134/S0026261707040133
Ключевые слова (''Своб.индексиров.''): anoxygenic photosynthesis--anoxygenic phototrophic bacteria--meromictic brackish lakes--ahrensia kielensis--bacteria (microorganisms)--chlorobi--chlorobium--chlorobium limicola--chromatiaceae--lamprocystis purpurea--photobacteria--proteobacteria--rhodomicrobium--rhodovulum--rhodovulum strictum--thiocapsa roseopersicina
Аннотация: The anoxygenic phototrophic bacterial community of the brackish meromictic Lake Shira (Khakassia) was investigated in August 2001, July 2002, and February-March 2003. In all the periods of investigation, the prevailing microorganisms were purple sulfur bacteria similar to Lamprocystis purpurea in morphology and pigment composition. Their highest number (3 ? 10 5 cells/ml) was recorded in July 2002 at the depth of 15 m. According to 16S rRNA gene analysis, the strain of purple sulfur bacteria isolated in 2001 and designated ShAm01 exhibited 98.6% similarity to the type strain of Thiocapsa roseopersicina and 97.1-94.4% similarity to the type strains of Tca. pendens, Tca. litoralis, and Tca. rosea. The minor microorganisms of the anoxygenic phototrophic bacterial community within the period of investigation were nonsulfur purple bacteria phylogenetically close to Rhodovulum strictum (98.3% similarity, strain ShRb01), Ahrensia kielensis (of 93.9% similarity, strain ShRb02), Rhodomicrobium vannieli (of 99.7% similarity, strain ShRmc01), and green sulfur bacteria, phylogenetically close to Chlorobium limicola (of 98.7% similarity, strain ShCl03). В© 2007 Pleiades Publishing, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T. G., Shumilova A. A., Shidlovskiy I. P., Nikolaeva E. D., Sukovatiy A. G., Vasiliev A. D., Shishatskaya E. I.
Заглавие : Antibacterial properties of films of cellulose composites with silver nanoparticles and antibiotics
Место публикации : Polym Test: Elsevier Ltd, 2018. - Vol. 65. - С. 54-68. - ISSN 01429418 (ISSN) , DOI 10.1016/j.polymertesting.2017.10.023
Ключевые слова (''Своб.индексиров.''): antibacterial activity--antibiotics--bacterial cellulose--composites--properties--silver nanoparticles--antibiotics--atoms--boron carbide--cell culture--cellulose--cellulose films--composite materials--escherichia coli--materials testing apparatus--metal nanoparticles--nanocomposite films--nanoparticles--scanning electron microscopy--silver compounds--spectrum analysis--synthesis (chemical)--tensile testing--water pollution--x ray analysis--anti-bacterial activity--antibacterial properties--bacterial cellulose--mechanical characteristics--properties--silver nanoparticles--structure and properties--tensile testing machines--silver--antibiotics--cellulose--composites--properties--silver
Аннотация: The present study describes production of bacterial cellulose composites with silver nanoparticles and antibiotics and compares their properties. Bacterial cellulose (BC) composites synthesized in the culture of the strain of acetic acid bacterium Komagataeibacter xylinus VKPM B-12068 with silver nanoparticles, BC/AgNps, were produced hydrothermally, under different AgNO3 concentrations (0.0001, 0.001, and 0.01 M) in the reaction medium. The presence of silver in the BC/AgNp composites was confirmed by elemental analysis conducted using scanning electron microscopy with a system of X-ray spectral analysis. Analysis showed that the average atomic number of silver particles in composite samples depended on the concentration of AgNO3: as AgNO3 concentration in the reaction solution was increased, silver content in the composites increased from 0.044 to 0.37 mg/cm2. BC composites with amikacin and ceftriaxone were prepared by immersing dry BC films in solutions containing different concentrations of the antibiotics. The surface structure and properties and physicochemical and mechanical characteristics of composites were investigated using SEM, DSC, X-ray analysis, the system for measuring water contact angles, and electromechanical tensile testing machine. The disk-diffusion method and the shake-flask culture method used in this study showed that all experimental composites had pronounced antibacterial activity against E. coli, Ps. eruginosa, K. pneumoniae, and St. aureus, and the BC/antibiotic composites were more active than BC/AgNp ones; S. aureus was the most susceptible to the effect of BC composites. No potential cytotoxicity was detected in any of the BC/AgNp composites in the NIH 3T3 mouse fibroblast cell culture, in contrast to the BC/antibiotic composites. These results suggest that BC composites constructed in the present study hold promise as dressings for managing wounds, including contaminated ones. © 2017 Elsevier Ltd
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Puzyr' A.P., Mogil'naya O.A., Tirranen L.S.
Заглавие : Architectonics of Flavobacterium sp. 56 and Flavobacterium sp. 22 colonies as exposed by transmission electron microscopy
Колич.характеристики :8 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1998. - Vol. 67, Is. 5. - P555-562. - ISSN 0026-2617
Примечания : Cited References: 21
Предметные рубрики: BACTERIAL
Ключевые слова (''Своб.индексиров.''): electron microscopy--colony architectonics--flavobacterium
Аннотация: Colonies of Flavobacterium sp. 56 and Flavobacterium sp. 22 were studied by electron microscopy without being washed off from solid nutrient medium and were shown to differ in the spatial arrangement and ultrastructure of their cells. Colonies of Flavobacterium sp. 56 contained a single type of cells, while those of Flavobacterium sp. 22 consisted of cells of two types differing in their ultrastructure. Within the colony volume, bacteria were oriented in a regular pattern characteristic of each strain studied. The evidence obtained relating to the ultrastructure, spatial orientation, and location of cells is sufficient for the unambiguous identification of bacterial strains and provides new information on colony architectonics.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Khizhnyak S. V., Petrushkina S. A., Chernov V. E., Ushakova S. A., Tikhomirov A. A.
Заглавие : Autochthonous microbial cenosis as a potential source of antagonistic strains for biological struggle against wheat fusarium in biotechnical life support systems
Место публикации : Aviakosmicheskaya Ekol. Med.: Slovo Ltd, 2020. - Vol. 54, Is. 3. - С. 84-91. - ISSN 0233528X (ISSN), DOI 10.21687/0233-528X-2020-54-3-84-91
Аннотация: The paper dwells upon the use of autochthonous microbial cinosis as a source of antagonistic strains for bioprotection of wheat against Fusarium in biotechnical life support systems (BT LSS). Six bacterial strains antagonistic to 9 phytopathogenic fungi Fusarium responsible for seedling blight of wheat in BT LSS were isolated from hydroponic solutions and artificial soils and subject to the genetic typing. Five strains represent bacteria g. Bacillus, one strain - Chryseobacterium. The 16S rRNA sequencing showed 94 to 100 % genetic affinity with typical cultures. All spore-forming strains-antagonists were capable of germinating and going through the whole development cycle In the presence of swelling wheat seeds. The isolates did not demonstrate antagonism to each other and can grow in a mixed culture. Spectra and levels of the antiobiotic activity of the antagonists, along with the Fusarium sensitivity to their antibiotic action, differed statistically (p 0.001). None of the antagonists was capable of suppressing the entire Fusarium spectrum detected in BT LSS individually. At the same time, treatment of seeds with a mixture of antagonists suppressed totally fungal development in wheat artificially inoculated by Fusarium conidia from a BT LSS roll culture, and had a statistical stimulating effect (p
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Altukhova O.V.
Заглавие : Autotrophic synthesis of polyhydroxyalkanoates by the bacteria Ralstonia eutropha in the presence of carbon monoxide
Колич.характеристики :4 с
Место публикации : Appl. Microbiol. Biotechnol.: SPRINGER-VERLAG, 2002. - Vol. 58, Is. 5. - P675-678. - ISSN 0175-7598, DOI 10.1007/s00253-002-0941-8
Примечания : Cited References: 35
Предметные рубрики: PSEUDOMONAS-OLEOVORANS
ALCALIGENES-EUTROPHUS
COAL
CULTIVATION
METABOLISM
SUBSTRATE
CULTURE
CO2
H-2
O-2
Аннотация: It has been found that the carbon monoxide (CO)-resistant strain of the hydrogen bacteria Ralstonia eutropha B5786 is able to synthesise polyhydroxyalkanoates (PHAs) in the presence of CO under autotrophic conditions. This strain, grown on model gas mixtures containing 5-25% CO v/v), accumulates up to 70-75% (of absolutely dry matter) PHA, without significant variation in the yield coefficient on hydrogen. No suppression of the activities of the key enzymes of PHA synthesis (beta-ketothiolase, acetoacetyl-CoA-reductase, butyrate dehydrogenase and poly-3-hydroxybutyrate synthase) was recorded. The PHA synthesised is a copolymer containing mostly beta-hydroxybutyrate (more than 99 mol%) with trace amounts of beta-hydroxyvalerate. The investigated properties of the polymer (molecular weight, crystallinity, temperature characteristics) do not differ from those of the polymer synthesised on electrolytic hydrogen.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Altukhova O.V.
Заглавие : Autotrophic synthesis of polyhydroxyalkanoates by the bacteria Ralstonia eutropha in the presence of carbon monoxide
Место публикации : Applied Microbiology and Biotechnology. - 2002. - Vol. 58, Is. 5. - С. 675-678. - ISSN 01757598 (ISSN) , DOI 10.1007/s00253-002-0941-8
Ключевые слова (''Своб.индексиров.''): 3 hydroxybutyric acid--acetoacetyl coenzyme a reductase--acetyl coenzyme a acyltransferase--beta hydroxyvalerate--butyrate dehydrogenase--carbon monoxide--electrolyte--hydrogen--oxidoreductase--poly(3 hydroxybutyric acid)--poly(3 hydroxybutyric acid)synthase--polyhydroxyalkanoic acid--polymer--unclassified drug--valeric acid--bacterium--article--autotrophy--bacterial growth--bacterial strain--biomass production--controlled study--crystallization--enzyme activity--molecular weight--nonhuman--synthesis--temperature--wautersia eutropha--carbon monoxide--culture media--cupriavidus necator--fatty acids--lipids--polyesters--bacteria (microorganisms)--negibacteria--ralstonia--wautersia eutropha
Аннотация: It has been found that the carbon monoxide (CO)-resistant strain of the hydrogen bacteria Ralstonia eutropha B5786 is able to synthesise polyhydroxy-alkanoates (PHAs) in the presence of CO under autotrophic conditions. This strain, grown on model gas mixtures containing 5-25% CO (v/v), accumulates up to 70-75% (of absolutely dry matter) PHA, without significant variation in the yield coefficient on hydrogen. No suppression of the activities of the key enzymes of PHA synthesis (?-ketothiolase, acetoacetyl-CoA-reductase, butyrate dehydrogenase and poly-3-hydroxybutyrate synthase) was recorded. The PHA synthesised is a copolymer containing mostly ?-hydroxybutyrate (more than 99 mol%) with trace amounts of ?-hydroxyvalerate. The investigated properties of the polymer (molecular weight, crystallinity, temperature characteristics) do not differ from those of the polymer synthesised on electrolytic hydrogen.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova, Tatiana G. G., Prudnikova, Svetlana V. V., Kiselev, Evgeniy G. G., Nemtsev, Ivan V. V., Vasiliev, Alexander D. D., Kuzmin, Andrey P. P., Shishatskaya, Ekaterina I. I.
Заглавие : Bacterial Cellulose (BC) and BC Composites: Production and Properties
Колич.характеристики :24 с
Коллективы : Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Место публикации : Nanomaterials: MDPI, 2022. - Vol. 12, Is. 2. - Ст.192. - ISSN 2079-4991(eISSN), DOI 10.3390/nano12020192
Примечания : Cited References:113. - This research was financially supported by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006.
Предметные рубрики: SILVER NANOPARTICLES
GLUCONACETOBACTER-HANSENII
MICROBIAL CELLULOSE
Аннотация: The synthesis of bacterial cellulose (BC) by Komagataeibacter xylinus strain B-12068 was investigated on various C-substrates, under submerged conditions with stirring and in static surface cultures. We implemented the synthesis of BC on glycerol, glucose, beet molasses, sprat oil, and a mixture of glucose with sunflower oil. The most productive process was obtained during the production of inoculum in submerged culture and subsequent growth of large BC films (up to 0.2 m(2) and more) in a static surface culture. The highest productivity of the BC synthesis process was obtained with the growth of bacteria on molasses and glycerol, 1.20 and 1.45 g/L per day, respectively. We obtained BC composites with silver nanoparticles (BC/AgNPs) and antibacterial drugs (chlorhexidine, baneocin, cefotaxime, and doripenem), and investigated the structure, physicochemical, and mechanical properties of composites. The disc-diffusion method showed pronounced antibacterial activity of BC composites against E. coli ATCC 25922 and S. aureus ATCC 25923.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Baker, Syed, Volova, Tatiana, Prudnikova, Svetlana, V, Shumilova, Anna A., Perianova, Olga, V, Zharkov, Sergey M., Kuzmin, And Rey, Olga, Kondratenka, Bogdan, Kiryukhin, Shidlovskiy, Ivan P., Potkina, Zoya K., Khohlova, Olga Y., Lobova, Tatiana, I
Заглавие : Bio-hybridization of nanobactericides with cellulose films for effective treatment against members of ESKAPE multi-drug-resistant pathogens
Колич.характеристики :10 с
Коллективы : Ministry of Education and Science of the Russian Federation under the scheme of 5-100: Russian Academic Excellence Project
Место публикации : Appl. Nanosci.: SPRINGER HEIDELBERG, 2018. - Vol. 8, Is. 5. - С. 1101-1110. - ISSN 2190-5509, DOI 10.1007/s13204-018-0717-9. - ISSN 2190-5517(eISSN)
Примечания : Cited References:51. - Authors are thankful for Ministry of Education and Science of the Russian Federation for providing funding under the scheme of 5-100: Russian Academic Excellence Project. Authors are grateful for facilities provided by Siberian Federal University to carry out the present study.
Предметные рубрики: SILVER NANOPARTICLES
BACTERIAL CELLULOSE
ANTIBIOTIC-RESISTANCE
Ключевые слова (''Своб.индексиров.''): eskape--bio-hybridization--silver nanobactericides--phytogenic--bactericidal activity
Аннотация: The rapid expansion of drug-resistant pathogens has created huge global impact and development of novel antimicrobial leads is one of the top priority studies in the current scenario. The present study aims to develop bio-hybridized nanocellulose films which comprise of phytogenic silver nanobactericides. The nanobactericides were synthesized by treating 1 mM silver nitrate with aqueous extract of Chamerion angustifolium which reduced the metal salt to produce polydispersed nanobactericides which were tested against the members of ESKAPE drug-resistant communities. The synthesized silver nanobactericides were subjected to characterization with UV-visible spectra which displayed maximum absorbance at 408 nm. The bio-molecular interaction of phyto-constituents to mediate synthesis and stabilization of nanobactericides was studied with Fourier-transform infrared spectroscopy (FTIR) which depicted functional groups associated with nanobactericides. The crystalline nature was studied with X-ray diffraction (XRD) which showed Bragg's intensities at 2 theta angle which denoted (111), (200), (220), and (311) planes. The morphological characteristics of silver nanobactericides were defined with transmission electron Microscopy (TEM) image which displayed polydispersity of silver nanobactericides with size ranging from 2 to 40 nm. The synthesized nanobactericides showed a significant activity against MRSA strain with 21 mm zone of inhibition. The minimal inhibitory concentration of silver nanobactericides to inhibit the growth of test pathogens was also determined which ranged between 0.625 and 1.25 mu g/ml. The silver nanobactericides were bio-hybridized onto nanocellulose films produced by Komagataeibacter xylinus B-12068 culture strain. The films were dried to determine the mechanical properties which showed increased in Young's modulus and tensile strength in comparison with control bacterial cellulose films. Overall, the results obtained in the present investigation are promising enough to report bactericidal activity of bio-hybridized nanobactericidal films against ESKAPE. These communities are reported to cause severe threats to all forms of lives irrespective to their habitats which can lead to huge economical crisis.
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16.

Вид документа : Статья из сборника (выпуск продолж. издания)
Шифр издания :
Автор(ы) : Kiselev E. G., Zhila N. O., Volova T. G.
Заглавие : Biodegradable polymers - Perspectives and applications in agriculture
Место публикации : IOP Conference Series: Earth and Environmental Science: IOP Publishing Ltd, 2021. - Vol. 689: 2020 International Conference on Germany and Russia: Ecosystems Without Borders, EcoSystConfKlgtu 2020 (5 October 2020 through 10 October 2020, ) Conference code: 167944, Is. 1. - Ст.012036. - , DOI 10.1088/1755-1315/689/1/012036
Аннотация: The paper presents a brief overview of the results of the implementation of the project "Agropreparations of the new generation: a strategy of construction and realization". The first part contains the analysis of the growth of the wild-type strain Cupriavidus necator B-10646 (formerly eutrophus) and the synthesis of polyhydroxyalkanoates by this strain on various substrates: glycerol, palm oil, Siberian oil seed, sunflower seed oils, and oleic acid. On refined glycerin, a highly productive process is implemented when scaling up, allowing to obtain 128 ± 11 g / L PHA. Evaluation of oils has shown that palm oil is the best carbon substrate. The second part presents the results of the development of environmentally friendly slow-release pesticide formulations. They are a degradable matrix of poly-3-hydroxybutyrate mixed with natural materials (peat, clay, wood flour), into which a pesticide (metribuzin, tribenuron-methyl, fenoxaprop-P-ethyl, azoxystrobin, epoxiconazole, and tebuconazole) has been. The developed preparations showed high activity against pathogenic fungi and weeds and had a much weaker negative effect on the soil microflora. Studies of the degradation of the developed preparations and the release of pesticides into the soil confirm their effectiveness over a long period of time, up to 90 days. © Published under licence by IOP Publishing Ltd.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mogilnaya O.A., Lobova T.I., Kargatova T.V., Popova L.Y.
Заглавие : Biofilm formation by bacterial associations under various salinities and copper ion stress
Колич.характеристики :9 с
Место публикации : Biofouling: TAYLOR & FRANCIS LTD, 2005. - Vol. 21, Is. 05.06.2013. - P247-255. - ISSN 0892-7014, DOI 10.1080/08924010500445848
Примечания : Cited References: 24
Предметные рубрики: HEAVY-METAL RESISTANCE
BACILLUS-SUBTILIS
PROTEIN
RISK
Ключевые слова (''Своб.индексиров.''): binary community--surface films--adhesion--copper--stress
Аннотация: The study addresses the effect of abiotic (medium salinity and copper ions) and biotic (interactions between populations) factors on the formation of structured communities by binary associations consisting of halotolerant bacteria (Alcaligenes sp. 1-1 or Acinetobacter sp. 1-19) and a wild-type B. subtilis 2335 strain or a transgenic strain. The results showed that 250 mg l(-1) of copper ions inhibit formation of biofilms by monocultures of the tested strains. Binary associations of the strains were more resistant to high concentrations (250 mg l(-1)) of copper ions. At the lowest NaCl concentration (0.05% and 2.5%) and in the presence of copper ions, bacilli seemed to help halotolerant bacteria survive. Under increased salinity and in the presence of copper ions, structured communities developed due to halotolerant bacteria. Coexistence under stressful conditions was beneficial for the both groups of bacteria.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Medvedeva S.E., Boyandin A., Lankin Y., Kotov D., Rodicheva E., Popova L.
Заглавие : BIOLUMBASE - The database of natural and transgenic bioluminescent organisms
Место публикации : Luminescence. - 2005. - Vol. 20, Is. 2. - С. 90-96. - ISSN 15227235 (ISSN) , DOI 10.1002/bio.809
Ключевые слова (''Своб.индексиров.''): bioluminescence--database--luminous bacteria--lux gene--marine--article--bacterial strain--bacterium culture--bacterium isolation--bioluminescence--data base--gene construct--medical information--transgenics--wide area network--bacteria--bacterial proteins--databases, factual--ecology--luminescence--luminescent proteins--marine biology--organisms, genetically modified--photobacterium--transgenes
Аннотация: The Institute of Biophysics SB RAS hosts and maintains a specialized collection of luminous bacteria (CCIBSO 836) containing over 700 strains isolated in various regions of the world's oceans. The culture collection is a source of lux genes and biologically active substances. The wide application of bioluminescence in medicine and ecology has given importance to analys-ing information on the structure and functioning of bioluminescence systems in natural and transgenic microorganisms, as well as on their features that are closely interrelated with bioluminescence. The aims of our BIOLUMBASE database are: gathering information on microorganisms with lux genes, their analysis and free access, and distribution of this data throughout the global network. The database includes two sections, natural and transgenic luminous microorganisms, and is updated by our own experimental results, the published literature and internet resources. For the future, a publicly available internet site for BIOLUMBASE is planned. This will list the strains and provide comprehensive information on the properties and functions of luminous bacteria, the mechanisms of regulation of bioluminescence systems, constructs with lux genes, and applications of bioluminescence in microbiology, ecology, medicine and biotechnology. It is noteworthy that this database will also be useful for evaluation of biological hazards of transgenic strains. Users will be able to carry out bibliographic and strain searches starting from any feature of interest. Copyright В© 2005 John Wiley & Sons, Ltd.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Burakova, Ludmila P., Kudryavtsev, Alexander N., Stepanyuk, Galina A., Baykov, Ivan K., Morozova, Vera V., Tikunova, Nina V., Dubova, Maria A., Lyapustin, Victor N., Yakimenko, Valeri V., Frank, Ludmila A.
Заглавие : Bioluminescent detection probe for tick-borne encephalitis virus immunoassay
Колич.характеристики :7 с
Коллективы : Russian Academy of Sciences, Siberian Branch [139], Russian Academy of Sciences [VI 57.1.1]
Место публикации : Anal. Bioanal. Chem.: SPRINGER HEIDELBERG, 2015. - Vol. 407, Is. 18. - С. 5417-5423. - ISSN 1618-2642, DOI 10.1007/s00216-015-8710-6. - ISSN 1618-2650(eISSN)
Примечания : Cited References:19. - The work was supported by the Russian Academy of Sciences, Siberian Branch, within the framework of the Interdisciplinary Integration Project No. 139 and the State budget allocated to the fundamental research at the Russian Academy of Sciences (project No. VI 57.1.1).
Предметные рубрики: COELENTERAZINE-BINDING PROTEIN
ENZYME-IMMUNOASSAY
RENILLA-MUELLERI
Ключевые слова (''Своб.индексиров.''): tick-borne encephalitis virus--single-chain antibody--luciferase--immunoassay
Аннотация: To facilitate the detection of the tick-borne encephalitis virus (TBEV), the causative agent of one of the most severe human neuroinfections, we have developed an immunoassay based on bioluminescent hybrid protein 14D5a-Rm7 as a detection probe. The protein containing Renilla luciferase as a reporter and a single-chain variable fragment (scFv) of murine immunoglobulin to TBEV as a recognition element was constructed, produced by bacterial expression, purified, and tested. Both domains were shown to reveal their specific biological properties-affinity to the target antigen and bioluminescent activity. Hybrid protein was applied as a label for solid-phase immunoassay of the antigens, associated with the tick-borne encephalitis virus (native glycoprotein E or extracts of the infected strain of lab ticks). The assay demonstrates high sensitivity (0.056 ng of glycoprotein E; 10(4)-10(5) virus particles or 0.1 pg virions) and simplicity and is competitive with conventional methods for detection of TBEV.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T., Menshikova O., Zhila N., Vasiliev A., Kiselev E., Peterson I., Shishatskaya E., Thomas S.
Заглавие : Biosynthesis and properties of P(3HB-co-3HV-co-3H4MV) produced by using the wild-type strain Cupriavidus eutrophus B-10646
Место публикации : Journal of Chemical Technology and Biotechnology. - 2018. - Article in press. - ISSN 02682575 (ISSN) , DOI 10.1002/jctb.5763
Ключевые слова (''Своб.индексиров.''): 3-hydroxy-4-methylvalerate--physicochemical properties--polyhydroxyalkanoates--synthesis--biochemistry--chemical industry--synthesis (chemical)--3-hydroxy-4-methylvalerate--crystalline-to-amorphous--degree of crystallinity--degrees of crystallinity--physicochemical property--polyhydroxyalkanoates--processing properties--wild-type strain--polymers
Аннотация: BACKGROUND: Polyhydroxyalkanoates (PHA) containing 3-hydroxy-4-methylvalerate (3H4MV) have better processing properties than other PHA types and were not prone to ageing. The main challenge in the production of these polymers is to simultaneously achieve high cell biomass, high polymer content, and high molar fraction of 3H4MV. The aim of this study was to investigate the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxy-4-methylvalerate) [P(3HB-co-3 HV-co-3H4MV)] by Cupriavidus eutrophus B-10646, synthesize polymers with different contents of 3H4MV and investigate their properties. RESULTS: It was shown that 4-methylvalerate (4MV, the precursor substrate) concentration in the culture medium must not be higher than 1 g L?1, and that 4MV must be carefully dosed, with at least 10–12 h intervals between supplementations. A series of polymers with molar fractions of 3H4MV between 2.7 and 11.3 mol% was synthesized. Degrees of crystallinity, molecular weight characteristics, and thermal properties of the polymers have been investigated as dependent on proportions of monomers. CONCLUSION: The content of 3H4MV depends on the concentration of 4MV and conditions of carbon nutrition. The molar fraction of 3H4MV had a strong effect on the crystalline to amorphous region ratio. PHA containing 3H4MV specimens synthesized in this study had a degree of crystallinity of below 50%, which remained unchanged for 2.5 years. © 2018 Society of Chemical Industry. © 2018 Society of Chemical Industry
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