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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Gribovskaya I.V.
Заглавие : A growth medium for the carboxydobacterium Seliberia carboxydohydrogena
Место публикации : Mikrobiologiya. - 1986. - Vol. 55, Is. 1. - С. 72-76. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): carbon monoxide--bacterial growth--culture medium--nonhuman
Аннотация: The growth of the carboxydobacterium Seliberia carboxydohydrogena Z-1062 was studied at a different concentration of mineral elements under the conditions of continuous turbidostat cultivation. The chemical composition of the bacterium was determined and the dynamics of its specific growth rate and the intracellular content of mineral elements were studied when the concentration of these elements was changed in the growth medium. The synthesis of 1 g of biomass by this strain required: N, 120 mg; P, 23 mg; S, 5.5 mg; K, 1.5 mg; Mg, 3.5 mg. Changes in the residual concentrations of the elements within a broad range had no effect on the specific rate of the carboxydobacterial growth and chemical composition.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Raibekas A.A.
Заглавие : Green flavoprotein from P. leiognathi: purification, characterization and identification as the product of the lux G(N) gene
Место публикации : Journal of bioluminescence and chemiluminescence. - 1991. - Vol. 6, Is. 3. - P169-176. - ISSN 08843996 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacterial protein--flavoprotein--amino acid sequence--article--bacterial gene--chemistry--genetics--isolation and purification--luminescence--molecular genetics--molecular weight--photobacterium--amino acid sequence--bacterial proteins--flavoproteins--genes, bacterial--luminescence--molecular sequence data--molecular weight--photobacterium--support, u.s. gov't, p.h.s.
Аннотация: A green flavoprotein (GFP) was isolated and purified to homogeneity from Photobacterium leiognathi, strain 208. GFP is a homodimer of molecular weight 54,000 and contains two molecules of an unusual flavin per molecule of protein. Various biochemical characteristics including isoelectric point, trypsin and chymotrypsin degradation, SDS and temperature influence on subunit dissociation and the dissociation of the flavin chromophore, were investigated. The sequence of 23 N-terminal amino acids was determined and found to be concurrent with the N-terminal amino acid sequence encoded by the lux G(N) gene of P. leiognathi. This fact suggests that GFP is a structural component of the Photobacterium luminescence system.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : POPOVA L.Y., LUTSKAYA N.I., BOGUCHAROV A.A., BRILKOV A.V., PECHURKIN N.S.
Заглавие : POPULATION-STRUCTURE DYNAMICS OF A RECOMBINANT STRAIN OF ESCHERICHIA-COLI IN CONTINUOUS CULTURE
Колич.характеристики :6 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1992. - Vol. 61, Is. 4. - С. 417-422. - ISSN 0026-2617
Примечания : Cited References: 10
Аннотация: We studied the population structure of Escherichia coli strain Z905, carrying the recombinant plasmid of the Ap(r)Lux+ phenotype, in continuous culture in a chemostat. We found that the maintenance of a stable, defined ratio of plasmidless to plasmid-carrying cells in the population depended primarily on the presence in the medium of the selective factor (ampicillin), and also on the nature of the growth-limiting carbon and energy source.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Gibson B.G., Lee J.
Заглавие : The yellow bioluminescence bacterium, Vibrio fischeri Y1, contains a bioluminescence active riboflavin protein in addition to the yellow fluorescence FMN protein
Место публикации : Biochemical and Biophysical Research Communications. - 1995. - Vol. 211, Is. 3. - С. 774-779. - ISSN 0006291X (ISSN) , DOI 10.1006/bbrc.1995.1880
Ключевые слова (''Своб.индексиров.''): riboflavin--article--bioluminescence--fluorescence--nonhuman--priority journal--protein analysis--protein synthesis--vibrio--vibrionaceae--bacterial proteins--chromatography, gel--chromatography, thin layer--flavin mononucleotide--flavoproteins--luminescence--riboflavin--spectrometry, fluorescence--support, u.s. gov't, p.h.s.--vibrio--bacteria (microorganisms)--photobacterium--vibrio--vibrio fischeri
Аннотация: The yellow bioluminescence Y1 strain of Vibrio fischeri can produce a 22 kDa protein with either FMN or riboflavin as a bound fluorophore. Both forms are active for shifting the bioluminescence spectral maximum. The fluorescence spectral distribution of the two proteins differs slightly and the in vivo emission appears to be an equal mixture of the two. The bioluminescence activity of the riboflavin Y1 protein contrasts with the inactivity of the related Photobacterium type.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Ketelaars M., Gibson B.G., Lee J.
Заглавие : Interaction of Photobacterium leiognathi and Vibrio fischeri Y1 luciferases with fluorescent (antenna) proteins: Bioluminescence effects of the aliphatic additive
Место публикации : Biochemistry. - 1996. - Vol. 35, Is. 37. - С. 12086-12093. - ISSN 00062960 (ISSN) , DOI 10.1021/bi9608931
Ключевые слова (''Своб.индексиров.''): luciferase--anisotropy--antenna--article--bioluminescence--complex formation--energy transfer--enzyme active site--enzyme kinetics--nonhuman--priority journal--protein protein interaction--spectroscopy--vibrionaceae--bacterial proteins--carrier proteins--cloning, molecular--dithionite--flavin mononucleotide--kinetics--luciferases--luminescent measurements--luminescent proteins--models, structural--photobacterium--protein binding--protein conformation--recombinant proteins--spectrophotometry--vibrio--bacteria (microorganisms)--photobacterium--photobacterium leiognathi--vibrio fischeri--vibrionaceae
Аннотация: The kinetics of the bacterial bioluminescence reaction is altered in the presence of the fluorescent (antenna) proteins, lumazine protein (LumP) from Photobacterium or the yellow fluorescence proteins (YFP) having FMN or Rf bound, from Vibrio fischeri strain Y1. Depending on reaction conditions, the bioluminescence intensity and its decay rate may be either enhanced or strongly quenched in the presence of the fluorescent proteins. These effects can be simply explained on the basis of the same protein-protein complex model that accounts for the bioluminescence spectral shifts induced by these fluorescent proteins. In such a complex, where the fluorophore evidently is in proximity to the luciferase active site, it is expected that the on off rate of certain aliphatic components of the reaction should be altered with a consequent shift in the equilibria among the luciferase intermediates, as recently elaborated in a kinetic scheme. These aliphatic components are the bioluminescence reaction substrate, tetradecanal or other long-chain aldehyde, its carboxylic acid product, or dodecanol used as a stabilizer of the luciferase peroxyflavin. No evidence can be found for the protein- protein interaction in the absence of the aliphatic component.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Gibson B.G., Lee J...
Заглавие : Direct measurement of excitation transfer in the protein complex of bacterial luciferase hydroxyflavin and the associated yellow fluorescence proteins from Vibrio fischeri Y1
Колич.характеристики :6 с
Место публикации : Biochemistry: AMER CHEMICAL SOC, 1996. - Vol. 35, Is. 25. - С. 8413-8418. - ISSN 0006-2960, DOI 10.1021/bi952691v
Примечания : Cited References: 24
Предметные рубрики: LUMAZINE PROTEIN
LUMINOUS BACTERIUM
STRAIN Y-1
BIOLUMINESCENCE
EMISSION
PURIFICATION
TRANSIENT
LIGHT
Аннотация: Time-resolved fluorescence was used to directly measure the energy transfer rate constant in the protein-protein complex involved in the yellow bioluminescence of Vibrio fischeri, strain Y1. In this reaction the putative donor is the fluorescent transient intermediate, luciferase hydroxyflavin, which exhibits a major fluorescence lifetime of the bound flavin of 10 ns. On addition of the acceptor, the V. fischeri yellow fluorescence protein containing either FMN or riboflavin as ligand, a rapid decay time, 0.25 ns, becomes predominant. The same results are observed using rec-luciferase from Photobacterium leiognathi to produce the donor. Because of favorable spectral separation in this system, this rapid decay rate of 4 ns(-1), can be directly equated to the energy transfer rate. This rate is ten times higher than the rate previously observed in the Photobacterium luciferase hydroxyflavin-lumazine protein, donor-acceptor system, derived from emission anisotropy measurements. This ten-times ratio is close to the ratio of spectral overlaps of the donor fluorescence with the acceptor absorption, between these two systems, so it is concluded that the topology of the protein complexes in both cases, must be very similar. Energy transfer is also monitored by the loss of steady-state fluorescence intensity at 460 nm of the donor, on addition of the acceptor protein. A fluorescence titration indicates that luciferase hydroxyflavin and the yellow protein complex with a 1:1 stoichiometry with a K-d of 0.7 mu M (0 degrees C). These parameters account for the bioluminescence spectral shifting effects observed in these reactions.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Ketelaars M..., Gibson B.G., Lee J...
Заглавие : Interaction of Photobacterium leiognathi and Vibrio fischeri Y1 luciferases with fluorescent (Antenna) proteins: Bioluminescence effects of the aliphatic additive
Колич.характеристики :8 с
Место публикации : Biochemistry: AMER CHEMICAL SOC, 1996. - Vol. 35, Is. 37. - С. 12086-12093. - ISSN 0006-2960, DOI 10.1021/bi9608931
Примечания : Cited References: 41
Предметные рубрики: BACTERIAL LUCIFERASE
LUMAZINE PROTEIN
FLAVIN INTERMEDIATE
ANGSTROM RESOLUTION
RIBOFLAVIN PROTEIN
PURIFICATION
MECHANISM
EMISSION
ALDEHYDE
INHIBITION
Аннотация: The kinetics of the bacterial bioluminescence reaction is altered in the presence of the fluorescent (antenna) proteins, lumazine protein (LumP) from Photobacterium or the yellow fluorescence proteins (YFP) having FMN or Rf bound, from Vibrio fischeri strain Y1, Depending on reaction conditions, the bioluminescence intensity and its decay rate may be either enhanced or strongly quenched in the presence of the fluorescent proteins. These effects call be simply explained on the basis of the same protein-protein complex model that accounts for the bioluminescence spectral shifts induced by these fluorescent proteins. In such a complex, when the fluorophore evidently is in proximity to the luciferase active site, it is expected that the on-off rate of certain aliphatic components of the reaction should be altered with a consequent shift in the equilibria among the luciferase intermediates, as recently elaborated in a kinetic scheme, These aliphatic components are the bioluminescence reaction substrate, tetradecanal or other long-chain aldehyde, its carboxylic acid product, or dodecanol used as a stabilizer of the luciferase peroxyflavin. No evidence can be found or the protein-protein interaction in the absence of the aliphatic component.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kargatova T.V., Maksimova E.E., Popova L.Yu., Bril'Kov A.V., Pechurkin N.S.
Заглавие : Phenotypic variability of the population of a recombinant luminescent strain of escherichia coli in aqueous microcosms
Место публикации : Mikrobiologiya. - 1997. - Vol. 66, Is. 1. - С. 101-106. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): article--escherichia coli--genetic recombination--genetics--luminescence--microbiology--penicillin resistance--phenotype--plasmid--ampicillin resistance--escherichia coli--luminescent measurements--phenotype--plasmids--recombination, genetic--water microbiology
Аннотация: The behavior of Escherichia coli Z905, carrying a recombinant plasmid pPHL7 with genes determining ampicillin resistance and bacterial luminescence, and the efficiency of expression of cloned genes were studied after introduction of the strain into model aqueous ecosystems with different trophic chain lengths. The E. coli Z905 variants isolated from ecosystems after different periods of time were found to vary in their resistance to ampicillin (from 50 to 0.05 ?g/ml) and in the intensity of bioluminescence. An increase in the concentration of the selective factor (ampicillin) or in the extent of the aqueous microcosm blooming restored the expression of the recombinant plasmid genes in some clones.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kargatova T.V., Maksimova E.E., Popova L.Yu., Bril'kov A.V., Pechurkin N.S.
Заглавие : Phenotypic variability of the population of a recombinant luminescent strain of escherichia coli in aqueous microcosms
Место публикации : Microbiology. - 1997. - Vol. 66, Is. 1. - С. 85-90. - ISSN 00262617 (ISSN)
Аннотация: The behavior of Escherichia coli Z905, carrying a recombinant plasmid pPHL7 with genes determining ampicillin resistance and bacterial luminescence, and the efficiency of expression of cloned genes were studied after introduction of the strain into model aqueous ecosystems with different trophic chain lengths. The E. coli Z905 variants isolated from ecosystems after different periods of time were found to vary in their resistance to ampicillin (from 50 to 0.05 ?g/ml) and in the intensity of bioluminescence. An increase in the concentration of the selective factor (ampicillin) or in the extent of the aqueous microcosm blooming restored the expression of the recombinant plasmid genes in some clones. В© 1997 MAHK Hayka/Interperiodica Publishing.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kargatova T.V., Maksimova E.E., Popova L.Y., Brilkov A.V., Pechurkin N.S.
Заглавие : Phenotypic variability of the population of a recombinant luminescent strain of Escherichia coli in aqueous microcosms
Колич.характеристики :6 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1997. - Vol. 66, Is. 1. - С. 85-90. - ISSN 0026-2617
Примечания : Cited References: 12
Предметные рубрики: GENETICALLY-MODIFIED MICROORGANISMS
Аннотация: The behavior of Escherichia coil Z905, carrying a recombinant plasmid pPHL7 with genes determining ampicillin resistance and bacterial luminescence, and the efficiency of expression of cloned genes were studied after introduction of the strain into model aqueous ecosystems with different trophic chain lengths. The E. coli Z905 variants isolated from ecosystems after different periods of time were found to vary in their resistance to ampicillin (from 50 to 0.05 mu g/ml) and in the intensity of bioluminescence. An increase in the concentration of the selective factor (ampicillin) or in the extent of the aqueous microcosm blooming restored the expression of the recombinant plasmid genes in some clones.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Maksimova E.E., Popova L.Y., Kargatova T.V., Shpagina V.V.
Заглавие : Controlled expression of bacterial luminescence genes cloned in a multicopy recombinant plasmid
Колич.характеристики :4 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1997. - Vol. 66, Is. 2. - P184-187. - ISSN 0026-2617
Примечания : Cited References: 17
Предметные рубрики: GENETICALLY-MODIFIED MICROORGANISMS
BIOLUMINESCENCE
ENVIRONMENT
Ключевые слова (''Своб.индексиров.''): recombinant plasmid--escherichia coli--luminescence--catabolite repression
Аннотация: Luminescence and growth responses of the recombinant strain Escherichia coil Z905 (Ap(r)Lux(+)) to different concentrations of ampicillin depended on the source of carbon and energy. When glycerol was used as the substrate, the intensity of luminescence rose with the ampicillin concentration in the medium. Glucose caused catabolite repression of cell luminescence up to the late stationary phase, and ampicillin enhanced this effect. The feasibility of controlling the expression of cloned lux genes was shown.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Popova L.Y., Kargatova T.V., Maksimova E.E., Belyavskaya V.A.
Заглавие : A study on the possibility of environmental adaptation of a Bacillus subtilis strain containing a recombinant plasmid with the gene of human interferon alpha 2
Колич.характеристики :5 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1997. - Vol. 66, Is. 6. - P637-641. - ISSN 0026-2617
Примечания : Cited References: 13
Предметные рубрики: GENETICALLY-MODIFIED MICROORGANISMS
Ключевые слова (''Своб.индексиров.''): microecosystem--bacillus subtilis--recombinant plasmid--interferon--adaptation
Аннотация: Adaptation of the Bacillus subtilis strain 2335/105 (Km(r)Inf(+)) containing a recombinant plasmid encoding the extracellular human interferon alpha 2 was studied under various conditions. Stability of the plasmid in the population of B. subtilis 2335/105 was estimated under nonselective conditions. The plasmid-free cells and cells with a low number of plasmid copies were found to accumulate progressively, constituting 80% of the population after 10 culture passages, indicating the poor competitiveness of cells carrying a high number of plasmid copies. The behavior of vegetative cells of the recombinant strain introduced into aquatic microcosms differing in trophic chain length was studied. Within the first 10 days, the lysis of vegetative cells of B. subtilis 2335/105 occurred; the number of viable spores was very low but remained constant for half a year.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Lee J.
Заглавие : Purification and characterization of flavoproteins and cytochromes from the yellow bioluminescence marine bacterium Vibrio fischeri strain Y1
Место публикации : European Journal of Biochemistry. - 1997. - Vol. 245, Is. 3. - С. 790-796. - ISSN 00142956 (ISSN)
Ключевые слова (''Своб.индексиров.''): anisotropy--lumazine protein--photobacterium--thioredoxin reductase--time-resolved fluorescence--cytochrome--flavoprotein--article--bioluminescence--nonhuman--priority journal--protein analysis--protein purification--sea--vibrio--amino acid sequence--bacterial proteins--cytochromes--flavoproteins--molecular sequence data--sequence alignment--vibrio--azotobacter--bacteria (microorganisms)--escherichia coli--haemophilus--haemophilus influenza--murinae--negibacteria--photobacterium--photobacterium leiognathi--pseudomonas--uncultured marine bacterium--vibrio fischeri
Аннотация: Several flavoproteins and cytochromes that occur as major components in extracts of the yellow bioluminescence Y1 strain of the murine bacterium Vibrio fischeri have been purified and characterized with respect to their mass (SDS/PAGE) and matrix-assisted laser-desorption/ionization MS), chromatographic properties, N-terminal sequence, and spectroscopy (absorption, fluorescence emission and anisotropy decay). The investigated proteins were as follows: yellow fluorescence protein (YFP) with bound riboflavin, FMN or 6,7-dimethyl-8-ribityllumazine; a blue fluorescence protein (BFP) with bound 6,7-dimethyl-8-ribityllumazine, riboflavin, or 6- methyl-7-oxo-ribityllumazine; thioredoxin reductase with FAD as ligand; and two c-type diheme cytochromes, c551 and c554. We present evidence that the riboflavin-bound YFP has an N-terminal sequence corresponding to that published for the dimeric YFP. We show that an equilibrium replacement of the riboflavin can be made with excess lumazine derivative and that lumazine- bound YFP has different bioluminescence properties to those of the lumazine protein from Photobacterium leiognathi. BFP is a different protein again, and in the bacterial lysate it occurs in multiple forms, ligated to either riboflavin, lumazine, or t he 7-oxolumazine derivative. The N-terminal sequence for BFP-shows similarities to those of the YFP proteins and to lumazine protein and riboflavin synthase from Photobacterium. BFP in any form has no bioluminescence or riboflavin-synthase activity. A 70-kDa fluorescent flavoprotein with FAD as ligand has an N-terminal sequence highly similar to those of thioredoxin reductases from Haemophilus influenza and Escherichia coli. Cytochrome contaminations in previous preparations of YFP have been removed and an identified as the two c-type cytochromes c551 and c554. Both inhibit the NADH-induced bioluminescence in the reductase/luciferase system with the luciferase from P. leiognathi and V. fischeri. The N-terminal amino acid sequence of the cytochrome (c551) corresponds to a diheme cytochrome c4. The spectral properties of c554 are similar to those of other c5 cytochromes, and both c554 and c551 have absorption spectra similar to those of the respective cytochromes from the gram-negative bacteria Pseudomonas and Azotobacter.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Popova L.I., Maksimova E.E., Kargatova T.V., Bril'kov A.V., Pechurkin N.S.
Заглавие : Introduction and long-term storage of recombinant luminescent Escherichia coli strain Z905 in laboratory water microecosystems
Место публикации : Izvestiia Akademii nauk. Seriia biologicheskaia / Rossiiskaia akademiia nauk. - 1998. - Is. 6. - С. 670-677. - ISSN 10263470 (ISSN)
Ключевые слова (''Своб.индексиров.''): article--bacterial gene--chemoluminescence--escherichia coli--gene expression--genetic recombination--genetics--laboratory--microbiology--chemiluminescent measurements--escherichia coli--gene expression--genes, bacterial--laboratories--recombination, genetic--water microbiology
Аннотация: We studied preservation of recombinant Escherichia coli strain Z905 (AprLux+) in liquid microecosystems (LME) after the introduction. E. coli cells were shown to remain viable and preserve the ability to express the cloned lux genes for a long time (more than a year) in LME. The majority of the clones have reduced efficiency of the expression due to either changed regulation of the lux operon or decreased number of copies of the plasmid. These mechanisms could be realized either independently or simultaneously depending on LME conditions. We have exposed the major factors affecting the metabolic activity of the E. coli strain Z905 (AprLux+) introduced into model ecosystems and the level of expression of the cloned genes.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Zhilo N.O., Plotnikov V.F.
Заглавие : Physiological and biochemical properties of the alga Botryococcus braunii
Место публикации : Russian Journal of Plant Physiology. - 1998. - Vol. 45, Is. 6. - С. 775-779. - ISSN 10214437 (ISSN)
Ключевые слова (''Своб.индексиров.''): batch culture--botryococcus braunii--liquid hydrocarbons--productivity
Аннотация: The growth, productivity, and changes in the chemical composition of the green alga Botryococcus braunii Kutz H-252 were studied at different phases of growth in a luminostat culture. Modification of the Prat medium resulted in an increase in the alga yield to 3.9 g/l and a decrease in the generation time to 3-4 days. The specific growth rate during the exponential phase was 0.235/ day. These values are comparable with the best results obtained abroad. Among the pigments of B. braunii, besides chlorophylls a and b, carotene, lutein, neoxanthin, and violaxanthin were also identified. The highest content of these pigments, based on dry weight, was achieved during the exponential growth phase. During culture aging, there was an accumulation of carbohydrates and lipids in addition to a simultaneous decrease in the concentration of nitrogen-containing substances. It was discovered that this strain can synthesize liquid hydrocarbons. These hydrocarbons were found both in the alga biomass (ca. 1% of dry weight) and among the extracellular metabolites (up to 10.3 mg/l).
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Puzyr' A.P., Mogil'naya O.A., Tirranen L.S.
Заглавие : Architectonics of Flavobacterium sp. 56 and Flavobacterium sp. 22 colonies as exposed by transmission electron microscopy
Колич.характеристики :8 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1998. - Vol. 67, Is. 5. - P555-562. - ISSN 0026-2617
Примечания : Cited References: 21
Предметные рубрики: BACTERIAL
Ключевые слова (''Своб.индексиров.''): electron microscopy--colony architectonics--flavobacterium
Аннотация: Colonies of Flavobacterium sp. 56 and Flavobacterium sp. 22 were studied by electron microscopy without being washed off from solid nutrient medium and were shown to differ in the spatial arrangement and ultrastructure of their cells. Colonies of Flavobacterium sp. 56 contained a single type of cells, while those of Flavobacterium sp. 22 consisted of cells of two types differing in their ultrastructure. Within the colony volume, bacteria were oriented in a regular pattern characteristic of each strain studied. The evidence obtained relating to the ultrastructure, spatial orientation, and location of cells is sufficient for the unambiguous identification of bacterial strains and provides new information on colony architectonics.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Maksimova E.E., Popova L.Yu., Shpagina V.V., Belyavskaya V.A., Pechurkin N.S.
Заглавие : Effect of environmental factors on the expression of the catabolite-dependent lux-operon borne by a recombinant plasmid
Место публикации : Microbiology. - 1998. - Vol. 67, Is. 2. - С. 137-141. - ISSN 00262617 (ISSN)
Ключевые слова (''Своб.индексиров.''): catabolite repression--environmental factors--escherichia coli--introduction into model ecosystems--lux-operon--recombinant plasmid--regulation of expression
Аннотация: Expression of the lux-genes cloned in the recombinant plasmid pPHL7 (Ap1Lux+) in Escherichia coli Z905 cells was studied in various environments, including model aquatic ecosystems. Expression of the lux-genes strongly depended on the nutritional status of the medium. In particular, the cultivation of cells in nutrient-rich medium favored the maintenance of the initial level of expression of the lux-operon, whereas nutrient limitation induced recombinant cell variants with an impaired control of the catabolite-dependent luxoperon. On the other hand, long-term laboratory cultivation of the recombinant strain in nutrient-deficient media or its long-term life in model aquatic ecosystems led to the accumulation of cells with a stringent control on the cloned lux-genes in the bacterial population. The presence of the selective factor (ampicillin) in the medium had no significant effect on the expression of the lux-operon. В© 1998 MAHK Hayka/Interperiodica Publishing.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Maksimova E.E., Popova L.Yu., Shpagina V.V., Belyavskaya V.A., Pechurkin N.S.
Заглавие : Effect of environmental factors on the expression of the catabolite-dependent lux-operon borne by a recombinant plasmid
Место публикации : Mikrobiologiya. - 1998. - Vol. 67, Is. 2. - С. 170-175. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): catabolite repression--environmental factors--escherichia coli--introduction into model ecosystems--lux-operon--recombinant plasmid--regulation of expression--recombinant dna--article--bacterial gene--chemoluminescence--culture medium--escherichia coli--gene expression regulation--genetics--microbiology--molecular cloning--operon--plasmid--chemiluminescent measurements--cloning, molecular--culture media--dna, recombinant--escherichia coli--gene expression regulation, bacterial--genes, bacterial--operon--plasmids--water microbiology
Аннотация: Expression of the lux-genes cloned on the recombinant plasmid pPHL7 (Ap rLux +) in Escherichia coli Z905 cells was studied in various environments, including model aquatic ecosystems. Expression of the lux-genes strongly depended on the nutritional status of the medium. In particular, the cultivation of cells in nutrient-rich medium favored the maintenance of the initial level of expression of the lux-operon, whereas nutrient limitation induced recombinant cell variants with an impaired control of the catabolite-dependent luxoperon. On the other hand, long-term laboratory cultivation of the recombinant strain in nutrient-deficient media or its long-term life in model aquatic ecosystems led to the accumulation of cells with a stringent control on the cloned lux-genes in the bacterial population. The presence of the selective factor (ampicillin) in the medium had no significant effect on the expression of the lux-operon.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : U - Popova LYu, Pechurkin N.S., Maksimova E.E., Kargatova T.V. U - Krylova TYu, Lobova T.I., Boyandin A.N.
Заглавие : Experimental microcosms as models of natural ecosystems for monitoring survival of genetically modified microorganism.
Место публикации : Life support & biosphere science : international journal of earth space. - 1999. - Vol. 6, Is. 3. - С. 193-197. - ISSN 10699422 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacterial dna--recombinant dna--adaptation--article--ecosystem--escherichia coli--genetics--microbiology--plasmid--risk assessment--adaptation, biological--dna, bacterial--dna, recombinant--ecosystem--escherichia coli--microbiology--plasmids--risk assessment--soil microbiology--water microbiology
Аннотация: An experimental approach for investigation of genetically modified microorganisms (GMMO) introduced into model ecosystems to evaluate potential risk of propagation of recombinant plasmids in surrounding medium has been developed. The object of modeling was Escherichia coli Z905 strain with a recombinant plasmid with bacterial luminescence genes, which was introduced into water microcosms of different structure. The approach involves comprehensive investigation of GMMO at four hierarchical levels: molecular (retaining the structure of the plasmid and expression of cloned genes); cellular (variation of metabolic activity); population (competitive power and metabolic interactions of GMMO with indigenous microflora, migration of recombinant and natural plasmids); ecosystem (effect of GMMO and cloned genes on ecosystem parameters). The experimental evidence and theoretical estimates are intended to form grounds to develop a basic version of an ecological certificate for different GMMO variants.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pechurkin N.S., Brilkov A.V., Ganusov V.V., Kargatova T.V., Maksimova E.E., Popova L.Yu.
Заглавие : Modelling of genetically engineered microorganisms introduction in closed artificial microcosms
Место публикации : Advances in Space Research. - 1999. - Vol. 24, Is. 3. - С. 335-341. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(99)00320-8
Ключевые слова (''Своб.индексиров.''): aquatic environment--artificial ecosystem--ecological modeling--genetically modified organism--alga--animal--article--bacterial count--bacterial gene--biological model--biomass--escherichia coli--feasibility study--genetic engineering--genetics--growth, development and aging--microbiology--microclimate--photobacterium--plasmid--protozoon--time--yeast--algae--animals--biomass--colony count, microbial--ecological systems, closed--escherichia coli--feasibility studies--genes, bacterial--genetic engineering--models, biological--photobacterium--plasmids--protozoa--time factors--water microbiology--yeasts
Аннотация: The possibility of introducing genetically engineered microorganisms (GEM) into simple biotic cycles of laboratory water microcosms was investigated. The survival of the recombinant strain Escherichia coli Z905 (Ap(r), Lux+) in microcosms depends on the type of model ecosystems. During the absence of algae blooming in the model ecosystem, the part of plasmid-containing cells E.coli decreased fast, and the structure of the plasmid was also modified. In conditions of algae blooming (Ankistrodesmus sp.) an almost total maintenance of plasmid-containing cells was observed in E.coli population. A mathematics model of GEM's behavior in water ecosystems with different level of complexity has been formulated. Mechanisms causing the difference in luminescent exhibition of different species are discussed, and attempts are made to forecast the GEM's behavior in water ecosystems.
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