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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Khlusov I.A., Volova T.G.
Заглавие : A hybrid PHB-hydroxyapatite composite for biomedical application: Production, in vitro and in vivo investigation
Место публикации : Journal of Biomaterials Science, Polymer Edition. - 2006. - Vol. 17, Is. 5. - С. 481-498. - ISSN 09205063 (ISSN) , DOI 10.1163/156856206776986242
Ключевые слова (''Своб.индексиров.''): biocompatibility--hydroxyapatite (ha)--phb-hydroxyapatite composite--polyhydroxyalkanoate (pha)--polyhydroxybutyrate (p(3hb))--properties--biocompatibility--differential thermal analysis--electron microscopy--free energy--interfacial energy--physical properties--surface properties--x ray analysis--biomedical application--physicochemical properties--polyhydroxyalkanoate (pha)--polyhydroxybutyrate (phb)--hydroxyapatite--hydroxyapatite--poly(3 hydroxybutyric acid)--polymer--biomaterial--hydroxybutyric acid--adhesion--animal cell--animal tissue--article--biomedicine--bone marrow cell--cell differentiation--cell growth--chemical structure--composite material--controlled study--crystallization--decomposition--electron microscopy--in vitro study--in vivo study--melting point--mouse--nonhuman--ossification--osteoblast--physical chemistry--priority journal--rat--strength--structure analysis--surface property--synthesis--temperature measurement--thermal analysis--tissue engineering--wettability--animal--biomechanics--bioremediation--bone prosthesis--cattle--cell culture--chemistry--cytology--differential scanning calorimetry--drug effect--human--materials testing--prostheses and orthoses--scanning electron microscopy--standard--wistar rat--murinae--animals--biocompatible materials--biodegradation, environmental--biomechanics--bone substitutes--cattle--cells, cultured--differential thermal analysis--durapatite--humans--hydroxybutyrates--materials testing--microscopy, electron, scanning--osteoblasts--prostheses and implants--rats--rats, wistar--surface properties
Аннотация: Samples of a hybrid composite of polyhydroxybutyrate (PHB), a biodegradable polyester, and hydroxyapatite (HA), with different PHB/HA ratios, have been prepared using mechanical-physical method. Electron microscopy, X-ray structure analysis and differential thermal analysis have been used to investigate the structure and physicochemical properties of the composite, depending on the PHB/HA ratio. The properties of the surface of the HA-loaded composite are significantly different from those of the pure polymer. As the HA percentage in the composite increases, free interface energy, the cohesive force, i.e., the strength of the adhesive bond between the composite surface and the water phase, and surface wettability increase. The HA percentage of the composite does not influence its melting temperature, but affects the temperature for the onset of decomposition: as the HA content increases from 0 to 10% (w/w), Td decreases from 260В°C to 225В°C. The degree of crystallinity of PHB/HA increases from 77% to 89% with an increase in the HA fraction from 10% to 50%. Functional properties of the composites have been investigated in vitro and in vivo. The best parameters of growth and differentiation of murine marrow osteoblasts are registered on PHB/HA samples containing 10% and 20% HA. In ectopic bone formation assay it has been proven that the hybrid PHB/HA composites can function as scaffolds and that bone tissue develops on their surface and in pores. В© VSP 2006.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bolsunovsky A., Zotina T., Bondareva L.
Заглавие : Accumulation and release of 241Am by a macrophyte of the Yenisei River (Elodea canadensis)
Место публикации : Journal of Environmental Radioactivity. - 2005. - Vol. 81, Is. 1. - С. 33-46. - ISSN 0265931X (ISSN) , DOI 10.1016/j.jenvrad.2004.10.012
Ключевые слова (''Своб.индексиров.''): 241am--accumulation--laboratory experiments--release--submerged plant elodea canadensis--yenisei river--activation analysis--biomass--concentration (process)--effluents--plutonium--rivers--aquatic plants--elodea plant--macrophytes--radioactive contamination--transuranium elements--americium 241--river water--americium--americium nitrate--bioaccumulation--biological uptake--macrophyte--pollutant source--radioactive pollution--river water--submerged vegetation--article--biomass--concentration (parameters)--environmental factor--fractionation--laboratory--macrophyte--radioactivity--river--sampling--adsorption--chemistry--hydrocharitaceae--methodology--physiology--plant--radiation exposure--radiation monitoring--russian federation--sediment--soil pollutant--time--tissue distribution--water pollutant--eastern hemisphere--eurasia--russian federation--world--yenisei river--elodea canadensis--adsorption--americium--biomass--chemical fractionation--geologic sediments--hydrocharitaceae--plant shoots--radiation monitoring--rivers--russia--soil pollutants, radioactive--time factors--tissue distribution--water pollutants, radioactive
Аннотация: The source of radioactive contamination of the Yenisei River floodplain, including contamination with transuranic elements, is the Mining-and-Chemical Combine of the Russian Ministry of Atomic Energy, which has for many years been producing weapons-grade plutonium. Transuranic elements have been detected not only in the soil and sediment of the river but also in the biomass of aquatic plants. This work is an investigation of accumulation and release of 241Am by a submerged macrophyte of the Yenisei River (Elodea canadensis) in laboratory experiments. In 2000-2003, laboratory experiments were carried out with biomass of E. canadensis Mich. and filtered river water. The samples were collected from the Yenisei River upstream of the discharge of the Combine's radioactive effluent. The experiments showed that 241Am is accumulated by Elodea biomass: the activity concentration of 241Am can reach 3280 В± 240 Bq/g, with the concentration factor for 241Am 16 600 В± 2200 l/kg. Results of chemical fractionation have proved that in the course of 241Am accumulation by Elodea biomass, 241Am tightly bound to biomass increases from 11% to 27% of the total 241Am in the biomass. Release of 241Am from the decaying Elodea biomass has been evaluated experimentally. By the end of the experiment (lasting up to 127 days), the Elodea plants had lost up to 65% of their initial 241Am activity and the rate of 241Am release into the water environment reached 23 Bq/day. В© 2004 Elsevier Ltd. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Kamendov I.V., Starosvetsky S.I., Vinnik Y.S., Markelova N.N., Shageev A.A., Khorzhevsky V.A., Peryanova O.V., Shumilova A.A.
Заглавие : An in vivo study of osteoplastic properties of resorbable poly-3-hydroxybutyrate in models of segmental osteotomy and chronic osteomyelitis
Место публикации : Artif. Cells, Nanomed. Biotechnol. - 2014. - Vol. 42, Is. 5. - С. 344-355. - ISSN 21691401 (ISSN) , DOI 10.3109/21691401.2013.816312
Ключевые слова (''Своб.индексиров.''): 3d implants--bone substitute--histological analysis and radiography--hydroxyapatite--p3hb--polymer of 3-hydroxybutyric acid--reconstructive osteogenesis--3-hydroxybutyric acid--bone substitutes--histological analysis--osteogenesis--p3hb--hydroxyapatite
Аннотация: A series of 3D implants and filling materials prepared from powdered biodegradable polymers, polyhydroxyalkanoates (PHAs), have been designed for the purposes of reparative osteogenesis. The 3D implants are made of resorbable polymer of hydroxybutyric acid (poly-3-hydroxybutyrate, P3HB) and a composite of this polymer with hydroxyapatite (HA) (P3HB/HA). The properties of the implants were studied in vivo in a model of segmental osteotomy and compared with commercial material Bio-Oss®. All implants containing P3HB as the main component facilitate reconstructive osteogenesis. P3HB and P3HB/HA show pronounced osteoplastic properties; their in vivo degradation is slow and corresponds to the growth of a new bone tissue, facilitating normal reparative osteogenesis. Also, powdered P3HB and P3HB/tienam can be used as filling materials for osteoplasty of bone cavities infected by Staphylococcus aureus. Biodegradable 3D implants and P3HB-based filling materials show pronounced osteoplastic properties and degrade in vivo at a slow rate, enabling normal reparative osteogenesis.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Nikolaeva E.D., Goreva A.V., Brigham C.J., Volova T.G., Sinskey A.J.
Заглавие : An in vivo study of pha matrices of different chemical composition: Tissue reaction and biodegradation
Место публикации : Cellular Transplantation and Tissue Engineering. - 2012. - Vol. 7, Is. 1. - С. 73-80. - ISSN 1815445X (ISSN)
Ключевые слова (''Своб.индексиров.''): degradable polymer matrices--degradation--phas--polyhydroxyalkanoates--subcutaneous implantation--tissue reaction
Аннотация: The study addresses consequences of subcutaneous implantation of film matrices prepared from different PHAs to laboratory animals. No negative effects of subcutaneous implantation of PHA matrices on physiological and biochemical characteristics of the animals were determined. Independently of the matrices composition and duration of the contact with the internal environment of the organism we did not observe any deviations in the behavior of animals, their growth and development, as well as blood functions. Response of the tissues to PHA matrices was comparable with the response to polylactide, but substantially less expressed at the earlier time periods after implantation. Tissues response to implantation of PHA of all types is characterized by short-term (up to 2 weeks) post-traumatic inflammation with formation of fibrous capsules by 30th-60th days with the thickness less than 100 microns, which get thinner down to 40-60 microns by 180th day as the result of involution. No differences in response of tissues and the whole organism were observed for the matrices produced from the homopolymer of 3-hydroxybutyric acid (P3HB), copolymers of 3-hydroxybutyric and 4-hydroxybutyric acids (P3HB/4HB), 3-hydroxybutyric acid and 3-hydroxyvalerianic acids (P3HB/3HV), 3-hydroxybutyric and 3-hydroxyhexanoate acids (P3HB/3HH). Macrophages and foreign-body giant cells actively participate in the response of the tissues to PHAs. In the studied conditions matrices from the copolymers containing 3-hydroxyhexanoate and 4 hydroxybutyrate were determined as more actively degraded PHA. The next less degraded matrices were matrices from the copolymer of P3HB/3HV and the most resistant were P3HB matrices. The slower degradation of PHA matrices was accompanied by delayed development of giantcells response. The studied PHA matrices can be placed in the following range by their degradation: P3HB/3HH - P3HB/4HB - P3HB/HV - P3HB.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Sushchik N.N., Anishchenko O.V., Makhutova O.N., Kalachova G.S., Gribovskaya I.V.
Заглавие : Benefit-risk ratio of food fish intake as the source of essential fatty acids vs. heavy metals: A case study of Siberian grayling from the Yenisei River
Место публикации : Food Chemistry. - 2009. - Vol. 115, Is. 2. - С. 545-550. - ISSN 03088146 (ISSN) , DOI 10.1016/j.foodchem.2008.12.062
Ключевые слова (''Своб.индексиров.''): benefit-risk ratio--essential fatty acids--grayling--hazard quotient--heavy metals
Аннотация: A formula was derived for quantification of benefit-risk ratio (hazard quotient) for the intake of a product containing essential polyunsaturated fatty acids vs. heavy metals. The quotient was used in a three year case study of the contents of essential fatty acids and heavy metals in Siberian grayling, the main food fish from the middle section of the Yenisei River. As found, in general the fish intake was potentially very beneficial for human health, except on a few occasions, when the risk overweighed the benefit because of high contents of chromium in the fish muscle tissue. The data demonstrated the necessity for regular monitoring of the hazard quotients for food fish in wild conditions, based at least on monthly sampling frequency. В© 2008 Elsevier Ltd. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E., Goreva A., Kalacheva G., Volova T.
Заглавие : Biocompatibility and resorption of intravenously administered polymer microparticles in tissues of internal organs of laboratory animals
Место публикации : Journal of Biomaterials Science, Polymer Edition. - 2011. - Vol. 22, Is. 16. - С. 2185-2203. - ISSN 09205063 (ISSN) , DOI 10.1163/092050610X537138
Ключевые слова (''Своб.индексиров.''): 14c products of polymer resorption--intravenous administration--microparticles--resorbable polyhydroxybutyrate--tissues of internal organs--3-hydroxybutyric acid--sup14/supc products of polymer resorption--adverse effect--drug-delivery systems--growth and development--high molecular weight--initial molecular weight--initial values--internal organs--intravenous administration--laboratory animals--local response--matrix--mean diameter--micro-particles--polymer degradation--polymer microparticles--preparation process--radioactivity level--residual content--resorbable--sustained-release--tail veins--wistar rat--animals--biocompatibility--degradation--histology--molecular weight--morphology--radiation--radioactivity--rats--tissue--poly(3 hydroxybutyric acid)--animal experiment--animal tissue--article--biocompatibility--concentration process--controlled study--degradation--drug delivery system--female--gel permeation chromatography--heart--kidney parenchyma--liver--lung parenchyma--molecular weight--nonhuman--organ culture--priority journal--radioactivity--rat--spleen--animals--animals, laboratory--biocompatible materials--drug delivery systems--female--infusions, intravenous--materials testing--microspheres--molecular weight--particle size--polymers--rats--rats, wistar--tissue distribution--animalia--rattus--rattus norvegicus
Аннотация: Specimens of 14C-labeled polymer of 3-hydroxybutyric acid, P(3-HB), with different initial molecular weights, were used to prepare microparticles, whose morphology was not influenced by the M w of the polymer. During the particle preparation process, P(3-HB) molecular weight decreased by 15-20%. Sterile microparticles (mean diameter 2.4 ?m) were injected into the tail veins of Wistar rats (5 mg/rat). The effects of the particles administered to rats were studied based on the general response of animals and local response of internal organ tissues and blood morphology; no adverse effects on growth and development of the animals or unfavorable changes in the structure of the tissues of internal organs were observed. Measurements of radioactivity in tissues showed that 14C concentrations are different in different organs, changing during the course of the experiment. The main targets for 14C-labeled microparticles were tissues of the liver, spleen, and kidneys. Comparison of radioactivity levels and residual contents of high-molecular-weight matrix in tissues suggested that the most rapid metabolism and degradation of P(3-HB) occurred in the liver and spleen. Gel-permeation chromatography showed that at 3 h after the microparticles were injected into the bloodstream, polymer degradation started in all examined organs, except the lungs; at 12 weeks, the M w of the polymer matrix was as low as 20-30% of its initial value. The presence of high-molecular-weight (undegraded) polymer in the tissues at 12 weeks after administration of the particles suggests that P(3-HB) is degraded in tissues of internal organs slowly and, hence, P(3-HB)-based microparticles can be used as sustained-release drug-delivery systems. В© 2011 VSP.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Voinova O.N., Goreva A.V., Mogilnaya O.A., Volova T.G.
Заглавие : Biocompatibility of polyhydroxybutyrate microspheres: In vitro and in vivo evaluation
Место публикации : Journal of Materials Science: Materials in Medicine. - 2008. - Vol. 19, Is. 6. - С. 2493-2502. - ISSN 09574530 (ISSN) , DOI 10.1007/s10856-007-3345-6
Ключевые слова (''Своб.индексиров.''): biocompatibility--drug delivery--fibroblasts--ion implantation--microspheres--polymer matrix--fibroblast cells--hydroxybutyric acid--intramuscular implantation--polyhydroxybutyrate--organic polymers--microsphere--poly(3 hydroxybutyric acid)--polymer--animal cell--animal experiment--animal tissue--article--biocompatibility--cell infiltration--controlled study--giant cell--implantation--in vitro study--in vivo study--inflammation--macrophage--mouse--nonhuman--priority journal--rat--3-hydroxybutyric acid--animals--biocompatible materials--cell survival--inflammation--materials testing--mice--microspheres--necrosis--nih 3t3 cells--polymers--rats--rats, wistar--tetrazolium salts--thiazoles--time factors--rattus norvegicus
Аннотация: Microspheres have been prepared from the resorbable linear polyester of ?-hydroxybutyric acid (polyhydroxybutyrate, PHB) by the solvent evaporation technique and investigated in vitro and in vivo. Biocompatibility of the microspheres has been proved in tests in the culture of mouse fibroblast cell line NIH 3T3 and in experiments on intramuscular implantation of the microspheres to Wistar rats for 3 months. Tissue response to the implantation of polymeric microspheres has been found to consist in a mild inflammatory reaction, pronounced macrophage infiltration that increases over time, involving mono- and poly-nuclear foreign body giant cells that resorb the polymeric matrix. No fibrous capsules were formed around polymeric microparticles; neither necrosis nor any other adverse morphological changes and tissue transformation in response to the implantation of the PHB microparticles were recorded. The results of the study suggest that polyhydroxybutyrate is a good candidate for fabricating prolonged-action drugs in the form of microparticles intended for intramuscular injection. В© 2008 Springer Science+Business Media, LLC.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Remmel N.N., Kratasyuk V.A., Maznyak O.M., Inzhevatkin E.V., Nefedov V.P.
Заглавие : Bioluminescent analysis of intensity of pathological oxidative processes in cells of perfused rat liver after hyperthermia
Место публикации : Bulletin of Experimental Biology and Medicine. - 2003. - Vol. 135, Is. 1. - P43-45. - ISSN 00074888 (ISSN) , DOI 10.1023/A:1023489727703
Ключевые слова (''Своб.индексиров.''): bioluminescent analysis--controlled perfusion of isolated liver--hyperthermia oxidative stress--thiobarbituric acid reactive substance--animal experiment--animal model--animal tissue--article--bioluminescence--concentration (parameters)--controlled study--correlation analysis--female--hyperthermia--in vivo study--lipid peroxidation--liver metabolism--liver perfusion--male--nonhuman--oxidative stress--oxygen consumption--pathology--rat--thermal exposure--animals--female--hepatocytes--hyperthermia, induced--liver--luminescent measurements--male--oxidative stress--perfusion--rats--animalia
Аннотация: We studied the possibility of evaluation of the intensity of pathological oxidative processes in rat liver using an integral bioluminescent test with controlled perfusion of the isolated organ. The test revealed a significant correlation between the level of TBA-reactive products and bioluminescence intensity.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gealageas R..., Malikova N.P., Picaud S..., Borgdorff A.J., Burakova L.P., Brulet P..., Vysotski E.S., Dodd R.H.
Заглавие : Bioluminescent properties of obelin and aequorin with novel coelenterazine analogues
Колич.характеристики :13 с
Коллективы : ICSN; CNRS Physics, Chemistry and Biology interface grant; RFBR [12-04-00131]; Government of Russian Federation [11.G34.31.0058]; ANR
Место публикации : Anal. Bioanal. Chem.: SPRINGER HEIDELBERG, 2014. - Vol. 406, Is. 11. - С. 2695-2707. - ISSN 1618-2642, DOI 10.1007/s00216-014-7656-4. - ISSN 1618-2650
Примечания : Cited References: 57. - R.G. acknowledges the ICSN for a fellowship. We are grateful for the ANR grant to P.B. and a CNRS Physics, Chemistry and Biology interface grant to R.H.D. and P.B.; N.P.M, L.P.B., and E.S.V. acknowledge the RFBR grant 12-04-00131 and the Program of the Government of Russian Federation "Measures to attract leading scientists to Russian educational institutions" (grant 11.G34.31.0058). P.B. and A.J.B. are indebted to Eric Karplus from Science Wares Inc. for helping with single-photon imaging software.
Предметные рубрики: PHOTOPROTEIN OBELIN
CRYSTAL-STRUCTURE
CA2+-REGULATED PHOTOPROTEINS
CA2+-ACTIVATED PHOTOPROTEIN
SEMISYNTHETIC AEQUORINS
ANGSTROM RESOLUTION
RECOMBINANT OBELIN
BINDING PROTEIN
CALCIUM-BINDING
CA2+ DYNAMICS
Ключевые слова (''Своб.индексиров.''): bioluminescence--luciferase--photoprotein--coelenterazine
Аннотация: The main analytical use of Ca2+-regulated photoproteins from luminous coelenterates is for real-time non-invasive visualization of intracellular calcium concentration ([Ca2+](i)) dynamics in cells and whole organisms. A limitation of this approach for in vivo deep tissue imaging is the fact that blue light emitted by the photoprotein is highly absorbed by tissue. Seven novel coelenterazine analogues were synthesized and their effects on the bioluminescent properties of recombinant obelin from Obelia longissima and aequorin from Aequorea victoria were evaluated. Only analogues having electron-donating groups (m-OCH3 and m-OH) on the C6 phenol moiety or an extended resonance system at the C8 position (1-naphthyl and alpha-styryl analogues) showed a significant red shift of light emission. Of these, only the alpha-styryl analogue displayed a sufficiently high light intensity to allow eventual tissue penetration. The possible suitability of this compound for in vivo assays was corroborated by studies with aequorin which allowed the monitoring of [Ca2+](i) dynamics in cultured CHO cells and in hippocampal brain slices. Thus, the alpha-styryl coelenterazine analogue might be potentially useful for non-invasive, in vivo bioluminescence imaging in deep tissues of small animals.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T. G., Shumilova A. A., Nikolaeva E. D., Kirichenko A. K., Shishatskaya E. I.
Заглавие : Biotechnological wound dressings based on bacterial cellulose and degradable copolymer P(3HB/4HB)
Место публикации : Int. J. Biol. Macromol.: Elsevier B.V., 2019. - Vol. 131. - С. 230-240. - ISSN 01418130 (ISSN) , DOI 10.1016/j.ijbiomac.2019.03.068
Примечания : Cited By :1
Ключевые слова (''Своб.индексиров.''): bacterial cellulose--composites--polyhydroxyalkanoates--3 hydroxybutyric acid 4 hydroxybutyric acid copolymer--actovegin--bacterial cellulose--cellulose--collagen type 1--copolymer--cytokeratin 10--cytokeratin 14--solcoseryl--unclassified drug--angiogenesis--animal cell--animal experiment--animal model--animal tissue--biochemical analysis--biocompatibility--burn--cell growth--controlled study--cutaneous parameters--differential scanning calorimetry--drug release--elemental analysis--female--fibroblast--histopathology--in vitro study--in vivo study--inflammation--mechanics--nonhuman--rat--review--wound healing--wound planimetry--x ray analysis
Аннотация: Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] – a biodegradable polymer of microbial origin. Some of the experimental membranes were loaded with drugs promoting wound healing and epidermal cells differentiated from multipotent adipose-derived mesenchymal stem cells. A study has been carried out to investigate the structure and physical/mechanical properties of the membranes. The in vitro study showed that the most effective scaffolds for growing fibroblasts were composite BC/P(3HB/4HB) films loaded with actovegin. Two types of the experimental biotechnological wound dressings – BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts – were tested in vivo, on laboratory animals with model third-degree skin burns. Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing. Experimental wound dressings promoted healing more effectively than VoskoPran – a commercial wound dressing. © 2019
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11.

Вид документа : Статья из сборника (выпуск продолж. издания)
Шифр издания :
Автор(ы) : Boyandin A. N., Sukhanova A. A., Nikolaeva E. D., Sukovatyi A. G., Nemtsev I. V.
Заглавие : Blends of poly-3-hydroxybutyrate and poly-ε-caprolactone: Morphology, properties and biocompatibility
Место публикации : AIP Conference Proceedings: American Institute of Physics Inc., 2021. - Vol. 2388: 4th International Conference on Modern Synthetic Methodologies for Creating Drugs and Functional Materials, MOSM 2020 (16 November 2020 through 20 November 2020, ) Conference code: 174383. - Ст.020002. - , DOI 10.1063/5.0068677
Аннотация: A series of films was obtained from blends of poly-3-hydroxybutyrate (PHB) and poly-s-caprolactone (PCL). The structure, physical, mechanical and biological properties of the films were studied, depending on the ratio of the components. The microscopic structure of mixed films was determined by the main component of the blend: the films were porous with a predominance of PHB and relatively smooth with a predominance of PCL. The highest values of elongation at break (229-269%), as well as the local maximum values of tensile strength (14-15 MPa) were noted for blends with 5-25% PHB content. At PHB content of 25% and 75%, local maxima of Young's modulus were observed (507 and 655 MPa, respectively). When the ratio of the components in the blend was equal (50:50 by weight), the films were heterogeneous and had the lowest values of mechanical strength (ultimate strength and elongation at break were 3 MPa and 15.5%, respectively). A study of the biocompatibility of mixed films in a culture of NIH 3T3 mouse fibroblasts based on determination of the number of adhered cells in the MTT test showed the absence of cytotoxic action, as well as high adhesion and cell proliferation, in some cases exceeding the data for films from pure PHB and PCL. This demonstrates the suitability of PHB-PCL blends for the production of cell carriers for tissue engineering and other reconstructive technologies. © 2021 Author(s).
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M. I., Glushchenko L. A., Makhutova O. N., Rudchenko A. E., Shulepina S. P., Dubovskaya O. P., Zuev I. V., Kolmakov V. I., Sushchik N. N.
Заглавие : Comparative Analysis of Content of Omega-3 Polyunsaturated Fatty Acids in Food and Muscle Tissue of Fish from Aquaculture and Natural Habitats
Колич.характеристики :12 с
Коллективы : Russian Foundation for Basic Research [16-14-10001]
Место публикации : Contemp. Probl. Ecol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2018. - Vol. 11, Is. 3. - С. 297-308. - ISSN 1995-4255, DOI 10.1134/S199542551803006X. - ISSN 1995-4263(eISSN)
Примечания : Cited References:86. - This study was supported by the Russian Foundation for Basic Research, project no. 16-14-10001. We are grateful to A.A. Makhrov and E.A. Borovikova for assisting in material collection and for useful comments to the initial text of the article.
Предметные рубрики: LONG-CHAIN OMEGA-3-FATTY-ACIDS
TROUT ONCORHYNCHUS-MYKISS
BREAM
Ключевые слова (''Своб.индексиров.''): fatty acids--aquaculture--food chain--bioaccumulation
Аннотация: Two fish species reared in aquaculture (pink salmon Oncorhynchus gorbuscha and whitefish Coregonus lavaretus) and ten fish species from natural habitats (whitefish C. lavaretus, tugun Coregonus tugun, broad whitefish Coregonus nasus, least cisco Coregonus sardinella, vendace Coregonus albula, boganid charr Salvelinus boganidae, charr Salvelinus alpinus complex, northern pike Esox lucius, sharp-snouted lenok Brachymystax lenok, and taimen Hucho taimen) have been studied. The content of two long-chain polyunsaturated omega-3 fatty acids (PUFAs), eicosapentaenoic acid (20:5n-3, EPA) and docosahexaenoic acid (22:6n-3, DHA), in the muscle tissue of the fish and in their food (intestine contents) are compared. In the aquacultures of whitefish and pink salmon, the total content of EPA and DHA is significantly higher in feed than in the muscle tissue of the fish, which indicates losses of PUFA in the two-link food chain of the aquaculture during their transfer to the upper trophic level. EPA and DHA losses in aquaculture, which are confirmed by numerous literature data, mean an inefficient usage of the available sources of PUFAs and the aggravation of the global deficit of these biochemicals in the human diet. A study of natural fish populations reveals the accumulation of EPA and DHA in their biomass compared to food in many cases, although opposite phenomena are also observed. An assumption on the presence of an optimal, physiologically adequate species-specific level of PUFA in the fish muscle tissue has been made based on our data and literature data. If the level of PUFAs in the muscles is lower than optimal, their accumulation (bioaccumulation) from food and/or de novo synthesis are observed. When the optimal level is exceeded, the content of EPA and DHA in biomass approaches maximum species-specific values; however, part of these PUFAs entering from food is not digested or is catabolized. According to the obtained data, the species of the order Salmoniformes have an optimal level of 2 to 6 mg/g of wet weight. It has been found that in aquaculture approaching to maximum values of EPA + DHA content was accompanied by their losses (scattering) in the food chains, while in natural ecosystems the maximum values of PUFA content in the fish biomass are achieved by their accumulation from the lower trophic level. Boganid charr S. boganidae had the highest content of EPA + DHA in the muscle tissue among all known fish species (32.78 mg/g of wet weight).
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nikolaeva E.D., Shishatskaya E.I., Mochalov K.E., Volova T.G., Sinsky A.J.
Заглавие : Comparative investigation of polyhydroxyalkanoate scaffolds with various chemical compositions
Место публикации : Cellular Transplantation and Tissue Engineering. - 2011. - Vol. 6, Is. 4. - С. 54-63. - ISSN 1815445X (ISSN)
Ключевые слова (''Своб.индексиров.''): biopolymers--scaffolds--tissue engineering
Аннотация: The authors have constructed and characterized a series of membranes based on resorbable polyhydroxyalkanoates of different compositions. Five PHA types have been studied: a homopolymer of 3-hydroxybutyric acid, copolymers of 3-hydroxybutyric and 4-hydroxybutyric acids, 3-hydroxybutyric and 3-hydroxyvaleric acids, 3-hydroxybutyric and 3-hydroxyhexanoic acids. Scanning electron microscopy and atomicforce microscopy were used to examine the microstructure of membrane surfaces, showing that membranes based on the copolymer of 3-hydroxybutyrate and 3-hydroxyhexanoate had the roughest surface, while membranes based on the copolymer of 3-hydroxybutyrate and 3-hydroxyvalerate had the smoothest surface. The contact angle for water in air was smaller and hydrophilic properties better in the copolymer membranes than in the membranes based on the high-crystallinity homopolymer of 3-hydroxybutyric acid. The culture of mouse fibroblast cell line NIH 3,3 was used to test PHAbased membranes; results of fluorescent probes of DNA DAPI and the MTT assay show that membranes based on studied PHAs are not cytotoxic on direct contact with cells and are highly biocompatible; their adhesive properties and ability to maintain fibroblast proliferation are similar to those of polystyrene and better than those of polylactic acid membranes.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik, Nadezhda N., Makhutova, Olesia N., Rudchenko, Anastasia E., Glushchenko, Larisa A., Shulepina, Svetlana P., Kolmakova, Anzhelika A., Gladyshev, Michail I.
Заглавие : Comparison of Fatty Acid Contents in Major Lipid Classes of Seven Salmonid Species from Siberian Arctic Lakes
Колич.характеристики :18 с
Коллективы : Russian Science FoundationRussian Science Foundation (RSF) [16-14-10001]
Место публикации : Biomolecules: MDPI, 2020. - Vol. 10, Is. 3. - Ст.419. - ISSN 2218-273X(eISSN), DOI 10.3390/biom10030419
Примечания : Cited References:60. - This research was funded by the Russian Science Foundation, grant number 16-14-10001.
Предметные рубрики: LONG-CHAIN
FISH
QUALITY
OMEGA-3
MARINE
WILD
TISSUE
FOOD
Аннотация: Long-chain omega-3 polyunsaturated fatty acids (LC-PUFA) essential for human nutrition are mostly obtained from wild-caught fish. To sustain the LC-PUFA supply from natural populations, one needs to know how environmental and intrinsic factors affect fish fatty acid (FA) profiles and contents. We studied seven Salmoniformes species from two arctic lakes. We aimed to estimate differences in the FA composition of total lipids and two major lipid classes, polar lipids (PL) and triacylglycerols (TAG), among the species and to evaluate LC-PUFA contents corresponding to PL and TAG in muscles. Fatty acid profiles of PL and TAG in all species were characterized by the prevalence of omega-3 LC-PUFA and C16-C18 monoenoic FA, respectively. Fish with similar feeding spectra were identified similarly in multivariate analyses of total lipids, TAG and PL, due to differences in levels of mostly the same FA. Thus, the suitability of both TAG and total lipids for the identification of the feeding spectra of fish was confirmed. All species had similar content of LC-PUFA esterified as PL, 1.9-3.5 mg g(-1), while the content of the TAG form strongly varied, from 0.9 to 9.8 mg g(-1). The LC-PUFA-rich fish species accumulated these valuable compounds predominately in the TAG form.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Lepskaya E.V., Sushchik N.N., Makhutova O.N., Kalachova G.S., Malyshevskaya K.K., Markevich G.N.
Заглавие : Comparison of Polyunsaturated Fatty Acids Content in Filets of Anadromous and Landlocked Sockeye Salmon Oncorhynchus Nerka
Место публикации : Journal of Food Science. - 2012. - Vol. 77, Is. 12. - С. C1307-C1310. - ISSN 00221147 (ISSN) , DOI 10.1111/j.1750-3841.2012.02998.x
Ключевые слова (''Своб.индексиров.''): kokanee salmon--polyunsaturated fatty acids--sockeye salmon--docosahexaenoic acid--icosapentaenoic acid--animal--article--body weight--classification--comparative study--female--male--phylogeny--russian federation--salmon--animals--body weight--docosahexaenoic acids--eicosapentaenoic acid--female--male--phylogeny--russia--salmon--oncorhynchus nerka
Аннотация: Abstract: Fatty acid composition and content of 2 forms of sockeye salmon Oncorhynchus nerka from lakes in Kamchatka Peninsula (Russia) were compared. One form of sockeye salmon was anadromous ("marine"), that is, adult fish migrated in ocean to feed and grow and than return in the lake to breed. Fish of another form, kokanee, never migrate in the ocean. Per cent levels of the main indicators of nutritive value, eicosapentaenoic acid (EPA, 20:5n-3), and docosahexaenoic acid (DHA, 22:6n-3), were significantly higher in the landlocked O. nerka. However, concentrations of EPA and DHA per wet weight of filets were higher in the marine form, because of the relatively higher content of sum of fatty acids in their muscle tissue. As concluded, fish fed in marine environment had higher contents of long-chain n-3 fatty acids per wet weight than fish of the same species, fed in fresh waters. In general, both the anadromous sockeye salmon and the landlocked kokanee salmon can be recommended for human diet as a valuable product concerning contents of EPA and DHA. В© 2012 Institute of Food TechnologistsВ®.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N.N., Gladyshev M.I., Kalachova G.S., Makhutova O.N., Ageev A.V.
Заглавие : Comparison of seasonal dynamics of the essential PUFA contents in benthic invertebrates and grayling Thymallus arcticus in the Yenisei river
Место публикации : Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology. - 2006. - Vol. 145, Is. 3-4. - С. 278-287. - ISSN 10964959 (ISSN) , DOI 10.1016/j.cbpb.2006.05.014
Ключевые слова (''Своб.индексиров.''): benthic invertebrates--grayling--polyunsaturated fatty acids--riverine food web--essential fatty acid--long chain fatty acid--polyunsaturated fatty acid--amphipoda--animal tissue--annelid worm--article--benthos--chironomidae--comparative study--controlled study--dominant inheritance--ecosystem--evolution--fatty acid analysis--female--fish--fly--invertebrate--larva--male--metabolic regulation--nonhuman--nutrient dynamics--priority journal--river--seasonal variation--statistical significance--thymallus arcticus--animals--fatty acids, unsaturated--food chain--gastrointestinal contents--invertebrates--muscles--rivers--russia--salmonidae--seasons--amphipoda--chironomidae--diptera--gammaridae--invertebrata--oligochaeta (metazoa)--thymallus arcticus--trichoptera
Аннотация: Seasonal dynamics of contents of essential polyunsaturated fatty acids (PUFA) in dominant groups of benthic invertebrates: gammarids (Gammaridae, Amphipoda), oligochaetes (Oligochaeta), chironomid larvae (Chironomidae, Diptera) and caddisfly larvae (Trichoptera), and dominant benthivorous fish, Siberian grayling Thymallus arcticus, have been studied in ecosystem of the large Siberian river. During the year of the study most benthic invertebrate taxa showed significant variations in the contents of both C-18 and long chain C-20-22 PUFAs. In contrast, the fish, which consumed the zoobenthos, had no significant seasonal variations in long chain PUFAs' contents. Thereby, the fish, as organisms of relatively higher evolution level than invertebrates, was supposed to have more strict metabolic control of long chain PUFAs' contents. Evidence was obtained that the studied fish species, grayling, may be capable to convert dietary EPA into DHA. В© 2006 Elsevier Inc. All rights reserved.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N. N., Zuev I. V., Kalachova G. S., Ageev A. V., Gladyshev M. I.
Заглавие : Content of highly unsaturated fatty acids in fish from rivers of contrasting temperature
Колич.характеристики :10 с
Коллективы : Council on Grants from the President of the Russian Federation for Support of Leading Scientific Schools [NSh-9249.2016.5]; Russian Federal Tasks of Fundamental Research [51.1.1]; Federal Tasks of Ministry of Education and Science of the Russian Federation for Siberian Federal University [6.1504.2017/4.6]
Место публикации : River Res. Appl.: WILEY, 2018. - Vol. 34, Is. 6. - С. 565-574. - ISSN 1535-1459, DOI 10.1002/rra.3286. - ISSN 1535-1467(eISSN)
Примечания : Cited References:73. - Council on Grants from the President of the Russian Federation for Support of Leading Scientific Schools, Grant/Award Number: NSh-9249.2016.5; Russian Federal Tasks of Fundamental Research, Grant/Award Number: 51.1.1; Federal Tasks of Ministry of Education and Science of the Russian Federation for Siberian Federal University, Grant/Award Number: 6.1504.2017/4.6
Предметные рубрики: TROUT ONCORHYNCHUS-MYKISS
SALMON SALMO-SALAR
FRESH-WATER
LONG-CHAIN
Ключевые слова (''Своб.индексиров.''): benthivorous fish--climate warming--polyunsaturated fatty acids--river--water temperature
Аннотация: Contents of highly unsaturated long-chain fatty acids of omega-3 family (HUFA); eicosapentaenoic acid (EPA; 20:5n-3); and docosahexaenoic acid (DHA; 22:6n-3) are the principal indicators of the nutritive quality of fish for humans. Effects of environmental factors, first of all water temperature, on EPA and DHA contents in fish tissue are not currently completely understood. To reveal the putative effect of water temperature, fatty acid composition and contents were studied for 6 fish species, inhabiting the cold waters of the Yenisei River (Siberia, Russia) downstream of a dam (hypolimnetic release from reservoir) and its adjacent warm water tributaries (the Mana River and the Kacha River). It was hypothesized that (a) fish species from the cold river would have higher HUFA contents than fish from the warm rivers and (b) temperature would be negatively correlated with HUFA content in fish species. Using gas chromatography-mass spectrometry, distinct species-specific fatty acid profiles were observed, whereas contents of the essential fatty acids, EPA and DHA, in fish species from the cold Yenisei River were in general similar to that from warm tributaries. Thus, in contrast to the first hypothesis, phylogenetic factors overweighed the effect of water temperature and food (benthic invertebrate) composition, on fatty acid composition and HUFA content in fish. For the second hypothesis, for the 2 species inhabiting both cold and warm rivers, only one had higher EPA and DHA content at lower temperatures. Consequently, the response of EPA and DHA content in fish tissue to temperature variations may be species-specific.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Larionova, Marina D., Wu, Lijie, Eremeeva, Elena, V, Natashin, Pavel, V, Gulnov, Dmitry, V, Nemtseva, Elena, V, Liu, Dongsheng, Liu, Zhi-Jie, Vysotski, Eugene S.
Заглавие : Crystal structure of semisynthetic obelin-v
Колич.характеристики :16 с
Коллективы : National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [32011530076]; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [20-04-00085, 20-44-240006, 20-54-53011]
Место публикации : Protein Sci.: WILEY, 2021. - Article in press. - ISSN 0961-8368, DOI 10.1002/pro.4244. - ISSN 1469-896X(eISSN)
Примечания : Cited References:69. - National Natural Science Foundation of China, Grant/Award Number: 32011530076; Russian Foundation for Basic Research, Grant/Award Numbers: 20-04-00085, 20-44-240006, 20-54-53011
Предметные рубрики: CA2+-REGULATED PHOTOPROTEIN OBELIN
PHOTOLUMINESCENCE QUANTUM YIELD
Аннотация: Coelenterazine-v (CTZ-v), a synthetic derivative with an additional benzyl ring, yields a bright bioluminescence of Renilla luciferase and its "yellow" mutant with a significant shift in the emission spectrum toward longer wavelengths, which makes it the substrate of choice for deep tissue imaging. Although Ca2+-regulated photoproteins activated with CTZ-v also display red-shifted light emission, in contrast to Renilla luciferase their bioluminescence activities are very low, which makes photoproteins activated by CTZ-v unusable for calcium imaging. Here, we report the crystal structure of Ca2+-regulated photoprotein obelin with 2-hydroperoxycoelenterazine-v (obelin-v) at 1.80 angstrom resolution. The structures of obelin-v and obelin bound with native CTZ revealed almost no difference; only the minor rearrangement in hydrogen-bond pattern and slightly increased distances between key active site residues and some atoms of 2-hydroperoxycoelenterazine-v were found. The fluorescence quantum yield (phi(FL)) of obelin bound with coelenteramide-v (0.24) turned out to be even higher than that of obelin with native coelenteramide (0.19). Since both obelins are in effect the enzyme-substrate complexes containing the 2-hydroperoxy adduct of CTZ-v or CTZ, we reasonably assume the chemical reaction mechanisms and the yields of the reaction products (phi(R)) to be similar for both obelins. Based on these findings we suggest that low bioluminescence activity of obelin-v is caused by the low efficiency of generating an electronic excited state (phi(S)). In turn, the low phi(S) value as compared to that of native CTZ might be the result of small changes in the substrate microenvironment in the obelin-v active site.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Nikolaeva E.D., Shumilova A.A., Shabanov A.V., Volova T.G.
Заглавие : Cultivation of multipotent mesenchymal bone marrow cells on matrixes made of resorbable bioplastotan
Место публикации : Cellular Transplantation and Tissue Engineering. - 2013. - Vol. 8, Is. 1. - С. 57-65. - ISSN 1815445X (ISSN)
Ключевые слова (''Своб.индексиров.''): alkaline phosphatase--differentiation--extracellular calcium precipitates--multipotent mesenchymal stromal cells--osteopontin--resorbable bioplastotan
Аннотация: Series of cell carriers made of resorbable polyester Bioplastotan, which is a copolymer of 3- And 4-hydroxybutyric acid, are characterized in this work. With use of various technologies membranes, 3D scaffolds, and nanomatrixes formed by ultrafine fibers produced with electrostatic spinning were constructed. All types of scaffolds provide adhesion of multipotent mesenchymal stromal cells and are suitable for the cultivation and differentiation of cells in the osteoblastes, that confirmed by detection of extracellular calcium precipitates, alkaline phosphatase activity and osteopontin production in cell culture. В© Human stem cells institute, 2013.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Gordeev S.A., Puzyr A.P.
Заглавие : Degradation of P(3HB) and P(3HB-co-3HV) in biological media
Место публикации : Journal of Biomaterials Science, Polymer Edition. - 2005. - Vol. 16, Is. 5. - С. 643-657. - ISSN 09205063 (ISSN) , DOI 10.1163/1568562053783678
Ключевые слова (''Своб.индексиров.''): degradation rate--fiber properties--morphology--poly(hydroxybutyrate-co-hydroxyvalerate) (p(3hb-co-3hv))--polyhydroxyalkanoates(phas)--polyhydroxybutyrate (p(3hb))--copolymers--degradation--differential scanning calorimetry--enzymes--morphology--scanning electron microscopy--tensile strength--tissue--transmission electron microscopy--degradation rate--fiber properties--polyhydroxyalkanoates (phas)--polyhydroxybutyrate (p(3hb))--biopolymers--buffer--copolymer--poly(3 hydroxybutyric acid)--polyhydroxybutyrate hydroxyvalerate copolymer--unclassified drug--animal experiment--animal model--animal tissue--article--biodegradation--controlled study--crystal structure--fiber--giant cell--in vitro study--in vivo study--macrophage--morphology--nonhuman--ph--priority journal--rat--structure analysis--tensile strength--tissue water--weight reduction--animals--biodegradation, environmental--buffers--humans--hydrogen-ion concentration--hydroxybutyrates--macrophages--microscopy, electron, scanning--microscopy, electron, transmission--muscle, skeletal--polyesters--rats--rats, wistar
Аннотация: The biodegradability of oriented fibers made of polyhydroxybutyrate (P(3HB)) and its co-polymer with ?-hydroxyvalerate (P(3HB-co-3HV)) was investigated in buffer solutions and in biological media in vitro and in vivo. The fibers of both polymer types demonstrated resistance to hydrolytic degradation in buffer solutions at 38В°C and pH from 4.5 to 7.0 (for up to 180 days). It has been found that the biodegradation of the fibers in vitro in blood and serum and in vivo is accompanied by weight losses and minor changes in the microstructure with no significant losses in the tensile strength over a long time (up to 180 days). The biodegradation rate of the less crystalline co-polymer P(3HB-co-3HV) fibers was 1.4-2.0-times higher than that of the homopolymer P(3HB). It has also been shown that the degradation of the fibers in vivo is influenced both by tissue fluid enzymes and cells (macrophages and foreign-body giant cells). The fibers were eroded on the surface only with no gross defects and no dramatic effects on their mechanical performance. В© VSP 2005.
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