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1.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gros J.B., Lasseur Ch., Tikhomirov A.A., Manukovsky N.S., Kovalev V.S., Ushakova S.A., Zolotukhin I.G., Tirranen L.S., Karnachuk R.A., Dorofeev V.Yu.
Заглавие : Testing soil-like substrate for growing plants in bioregenerative life support systems
Место публикации : Advances in Space Research. - 2005. - Vol. 36, Is. 7. - С. 1312-1318. - ISSN 02731177 (ISSN) , DOI 10.1016/j.asr.2005.05.079
Ключевые слова (''Своб.индексиров.''): life support system--matter recycling--plants--soil-like substrate--biodiversity--correlation methods--growth kinetics--hormones--plants (botany)--recycling--soils--bioregeneration--life support system--matter recycling--soil-like substrate--space research
Аннотация: We studied soil-like substrate (SLS) as a potential candidate for plant cultivation in bioregenerative life support systems (BLSS). The SLS was obtained by successive conversion of wheat straw by oyster mushrooms and worms. Mature SLS contained 9.5% humic acids and 4.9% fulvic acids. First, it was shown that wheat, bean and cucumber yields as well as radish yields when cultivated on mature SLS were comparable to yields obtained on a neutral substrate (expanded clay aggregate) under hydroponics. Second, the possibility of increasing wheat and radish yields on the SLS was assessed at three levels of light intensity: 690, 920 and 1150 ?mol m-2 s-1 of photosynthetically active radiation (PAR). The highest wheat yield was obtained at 920 ?mol m-2 s-1, while radish yield increased steadily with increasing light intensity. Third, long-term SLS fertility was tested in a BLSS model with mineral and organic matter recycling. Eight cycles of wheat and 13 cycles of radish cultivation were carried out on the SLS in the experimental system. Correlation coefficients between SLS nitrogen content and total wheat biomass and grain yield were 0.92 and 0.97, respectively, and correlation coefficients between nitrogen content and total radish biomass and edible root yield were 0.88 and 0.87, respectively. Changes in hormone content (auxins, gibberellins, cytokinins and abscisic acid) in the SLS during matter recycling did not reduce plant productivity. Quantitative and species compositions of the SLS and irrigation water microflora were also investigated. Microbial community analysis of the SLS showed bacteria from Bacillus, Pseudomonas, Proteus, Nocardia, Mycobacterium, Arthrobacter and Enterobacter genera, and fungi from Trichoderma, Penicillium, Fusarium, Aspergillus, Mucor, Botrytis, and Cladosporium genera. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.
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2.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zolotukhin I.G., Tikhomirov A.A., Kudenko Yu.A., Gribovskaya I.V.
Заглавие : Biological and physicochemical methods for utilization of plant wastes and human exometabolites for increasing internal cycling and closure of life support systems
Место публикации : Advances in Space Research. - 2005. - Vol. 35, Is. 9 SPEC. ISS. - С. 1559-1562. - ISSN 02731177 (ISSN) , DOI 10.1016/j.asr.2005.01.006
Ключевые слова (''Своб.индексиров.''): blss--desalting--higher plants--nacl utilization--sls--biomass--crops--decomposition--electrodialysis--harvesting--metabolites--soils--wastes--blss--higher plants--nacl utilization--sls--plants (botany)--biomass--decay--deionization--harvesting--plants--soil--wastes--wheat--sodium chloride--article--biomass--bioremediation--culture medium--feces--growth, development and aging--human--metabolism--methodology--microbiology--microclimate--urine--waste management--wheat--biodegradation, environmental--biomass--culture media--ecological systems, closed--feces--humans--life support systems--sodium chloride--soil microbiology--triticum--urine--waste management
Аннотация: Wheat was cultivated on soil-like substrate (SLS) produced by the action of worms and microflora from the inedible biomass of wheat. After the growth of the wheat crop, the inedible biomass was restored in SLS and exposed to decomposition ("biological" combustion) and its mineral compounds were assimilated by plants. Grain was returned to the SLS in the amount equivalent to human solid waste produced by consumption of the grain. Human wastes (urine and feces) after physicochemical processing turned into mineralized form (mineralized urine and mineralized feces) and entered the plants' nutrient solution amounts equal to average daily production. Periodically (once every 60-70 days) the nutrient solution was partly (up to 50%) desalinated by electrodialysis. Due to this NaCl concentration in the nutrient solution was sustained at a fixed level of about 0.26%. The salt concentrate obtained could be used in the human nutrition through NaCl extraction and the residuary elements were returned through the mineralized human liquid wastes into matter turnover. The control wheat cultivation was carried out on peat with use of the Knop nutrient solution. Serial cultivation of several wheat vegetations within 280 days was conducted during the experiment. Grain output varied and yield/harvest depended, in large part, upon the amount of inedible biomass returned to SLS and the speed of its decomposition. After achieving a stationary regime, (when the quantity of wheat inedible biomass utilized during vegetation in SLS is equal to the quantity of biomass introduced into SLS before vegetation) grain harvest in comparison with the control was at most 30% less, and in some cases was comparable to the control harvest values. The investigations carried out on the wheat example demonstrated in principle the possibility of long-term functioning of the LSS photosynthesizing link based on optimizations of biological and physicochemical methods of utilization of the human and plants wastes. The possibilities for the use of these technologies for the creation integrated biological-physicochemical LSS with high closure degree of internal matter turnover are discussed in this paper. В© 2005 Published by Elsevier Ltd on behalf of COSPAR.
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3.

Вид документа : Статья из сборника (выпуск монографической серии)
Шифр издания :
Автор(ы) : Gros J.-B., Lasseur C., Tikhomirov A.A., Manukovsky N.S., Ushakova S.A., Zolotukhin I.G., Gribovskaya I.V., Kovalev V.S.
Заглавие : Soil-like substrate for plant growing derived from inedible plant mass: Preparing, composition, fertility
Место публикации : Acta Horticulturae. - 2004. - Vol. 644. - С. 151-155
Ключевые слова (''Своб.индексиров.''): fertility--hydroponics--oyster mushroom--phytomass yield--wheat straw--worms
Аннотация: Fertility of soil-like substrate (SLS) made by successive conversion of wheat straw by oyster mushrooms and worms has been evaluated. Soil-like substrate of different degree of maturity has been tested. The most ready SLS provided the higher yields of wheat. It comprised 9.5% of humic acids, 4.9% of fulvic acids and 15.2% of nonhydrolyzable substances. At atmospheric concentration of carbon dioxide the soil-like substrate decreased its mass over the vegetation period by 14- 21%. The yield of wheat, beans and cucumbers grown on the soil-like substrate was compared to that on hydroponics.
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4.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tikhomirov A.A., Ushakova S.A., Manukovsky N.S., Lisovsky G.M., Kudenko Yu.A., Koyalev V.S., Gribovskaya I.V., Tirranen L.S., Zolotukhin I.G., Gros J.B., Lasseur Ch.
Заглавие : Synthesis of biomass and utilization of plants wastes in a physical model of biological life-support system
Место публикации : Acta Astronautica. - 2003. - Vol. 53, Is. 4-10. - С. 249-257. - ISSN 00945765 (ISSN) , DOI 10.1016/S0094-5765(03)00137-1
Ключевые слова (''Своб.индексиров.''): ecosystems--microorganisms--ph--photosynthesis--plants (botany)--synthesis (chemical)--waste utilization--biological life support systems (blls)--gas exchange--plant respiration--biomass--carbon dioxide--agaricales--article--biomass--bioremediation--growth, development and aging--hydroponics--incineration--metabolism--methodology--microbiology--microclimate--photosynthesis--plant physiology--radish--space flight--waste management--weightlessness--wheat--agaricales--biodegradation, environmental--biomass--carbon dioxide--ecological systems, closed--environmental microbiology--hydroponics--incineration--life support systems--photosynthesis--plant physiology--raphanus--space flight--triticum--waste management--weightlessness
Аннотация: The paper considers problems of biosynthesis of higher plants' biomass and "biological incineration" of plant wastes in a working physical model of biological LSS. The plant wastes are "biologically incinerated" in a special heterotrophic block involving Califomian worms, mushrooms and straw. The block processes plant wastes (straw, haulms) to produce soil-like substrate (SLS) on which plants (wheat, radish) are grown. Gas exchange in such a system consists of respiratory gas exchange of SLS and photosynthesis and respiration of plants. Specifics of gas exchange dynamics of high plants - SLS complex has been considered. Relationship between such a gas exchange and PAR irradiance and age of plants has been established. Nitrogen and iron were found to the first to limit plants' growth on SLS when process conditions are deranged. The SLS microflora has been found to have different kinds of ammonifying and denitrifying bacteria which is indicative of intensive transformation of nitrogen-containing compounds. The number of physiological groups of microorganisms in SLS was, on the whole, steady. As a result, organic substances - products of exchange of plants and microorganisms were not accumulated in the medium, but mineralized and assimilated by the biocenosis. Experiments showed that the developed model of a man-made ecosystem realized complete utilization of plant wastes and involved them into the intrasystem turnover. В© 2003 International Astronautical Federation. Published by Elsevier Science Ltd. All rights reserved.
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5.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tikhomirov A.A., Ushakova S.A., Manukovsky N.S., Lisovsky G.M., Kudenko Yu.A., Kovalev V.S., Gubanov V.G., Barkhatov Yu.V., Gribovskaya I.V., Zolotukhin I.G., Gros J.B., Lasseur Ch.
Заглавие : Mass exchange in an experimental new-generation life support system model based on biological regeneration of environment
Место публикации : Advances in Space Research. - 2003. - Vol. 31, Is. 7. - С. 1711-1720. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(03)80017-0
Ключевые слова (''Своб.индексиров.''): biomass--photosynthesis--plants (botany)--transpiration--mass exchange--life support systems (spacecraft)--ammonia--nitrogen--oxygen--biosphere--animal--annelid worm--article--biological model--biomass--bioremediation--evaluation--growth, development and aging--human--metabolism--microclimate--photosynthesis--pleurotus--radish--wheat--ammonia--animals--biodegradation, environmental--biomass--ecological systems, closed--evaluation studies--humans--life support systems--models, biological--nitrogen--oligochaeta--oxygen--photosynthesis--pleurotus--raphanus--triticum
Аннотация: An experimental model of a biological life support system was used to evaluate qualitative and quantitative parameters of the internal mass exchange. The photosynthesizing unit included the higher plant component (wheat and radish), and the heterotrophic unit consisted of a soil-like substrate, California worms, mushrooms and microbial microflora. The gas mass exchange involved evolution of oxygen by the photosynthesizing component and its uptake by the heterotroph component along with the formation and maintaining of the SLS structure, growth of mushrooms and California worms, human respiration, and some other processes. Human presence in the system in the form of "virtual human" that at regular intervals took part in the respirative gas exchange during the experiment. Experimental data demonstrated good oxygen/carbon dioxide balance, and the closure of the cycles of these gases was almost complete. The water cycle was nearly 100% closed. The main components in the water mass exchange were transpiration water and the watering solution with mineral elements. Human consumption of the edible plant biomass (grains and roots) was simulated by processing these products by a unique physicochemical method of oxidizing them to inorganic mineral compounds, which were then returned into the system and fully assimilated by the plants. The oxidation was achieved by "wet combustion" of organic biomass, using hydrogen peroxide following a special procedure, which does not require high temperature and pressure. Hydrogen peroxide is produced from the water inside the system. The closure of the cycle was estimated for individual elements and compounds. Stoichiometric proportions are given for the main components included in the experimental model of the system. Approaches to the mathematical modeling of the cycling processes are discussed, using the data of the experimental model. Nitrogen, as a representative of biogenic elements, shows an almost 100% closure of the cycle inside the system. The proposed experimental model of a biological system is discussed as a candidate for potential application in the investigations aimed at creating ecosystems with largely closed cycles of the internal mass exchange. The formation and maintenance of sustainable cycling of vitally important chemical elements and compounds in biological life support systems (BLSS) is an extremely pressing problem. To attain the stable functioning of biological life support systems (BLSS) and to maintain a high degree of closure of material cycles in them, it is essential to understand the character of mass exchange processes and stoichiometric proportions of the initial and synthesized components of the system. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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6.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Tikhomirov A.A., Ushakova S.A., Manukovsky N.S., Lisovsky G.M., Kudenko Y.A., Kovalev V.S., Gubanov V.G., Barkhatov Y.V., Gribovskaya I.V., Zolotukhin I.G., Gros J.B., Lasseur C...
Заглавие : Mass exchange in an experimental new-generation life support system model based on biological regeneration of environment
Место публикации : SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - С. 1711-1720. - 10. - ISBN 0273-1177, DOI 10.1016/S0273-1177(03)00108-X
Примечания : Cited References: 13
Аннотация: An experimental model of a biological life support system was used to evaluate qualitative and quantitative parameters of the internal mass exchange. The photosynthesizing unit included the higher plant component (wheat and radish), and the heterotrophic unit consisted of a soil-like substrate, California worms, mushrooms and microbial microflora. The gas mass exchange involved evolution of oxygen by the photosynthesizing component and its uptake by the heterotroph component along with the formation and maintaining of the SLS structure, growth of mushrooms and California worms, human respiration, and some other processes. Human presence in the system in the form of "virtual human" that at regular intervals took part in the respirative gas exchange during the experiment. Experimental data demonstrated good oxygen/carbon dioxide balance, and the closure of the cycles of these gases was almost complete. The water cycle was nearly 100% closed. The main components in the water mass exchange were transpiration water and the watering solution with mineral elements. Human consumption of the edible plant biomass (grains and roots) was simulated by processing these products by a unique physicochemical method of oxidizing them to inorganic mineral compounds, which were then returned into the system and fully assimilated by the plants. The oxidation was achieved by "wet combustion" of organic biomass, using hydrogen peroxide following a special procedure, which does not require high temperature and pressure. Hydrogen peroxide is produced from the water inside the system. The closure of the cycle was estimated for individual elements and compounds. Stoichiometric proportions are given for the main components included in the experimental model of the system. Approaches to the mathematical modeling of the cycling processes are discussed, using the data of the experimental model. Nitrogen, as a representative of biogenic elements, shows an almost 100% closure of the cycle inside the system, The proposed experimental model of a biological system is discussed as a candidate for potential application in the investigations aimed at creating ecosystems with largely closed cycles of the internal mass exchange. The formation and maintenance of sustainable cycling of vitally important chemical elements and compounds in biological life support systems (BLSS) is an extremely pressing problem. To attain the stable functioning of biological life support systems (BLSS) and to maintain a high degree of closure of material cycles in them, it is essential to understand the character of mass exchange processes and stoichiometric proportions of the initial and synthesized components of the system. (C) 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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7.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Aneli N.B., Belousova A.A., Saprunova V.B., Petushkov V.N., Kondrashov F.A., Plyuscheva M.V.
Заглавие : Ultrastructure and morphology of the bioluminescent system in "bioluminescent" and "non-bioluminescent" scale-worms (Polychaeta, Polynoidae)
Колич.характеристики :2 с
Место публикации : Luminescence: WILEY-BLACKWELL, 2014. - Vol. 29. - С. 41-42. - ISSN 1522-7235. - ISSN 1522-7243
Примечания : Cited References: 5
Предметные рубрики: ORGANS
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8.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Manukovsky N.S., Kovalev V.S., Rygalov V.Y., Zolotukhin I.G.
Заглавие : Waste bioregeneration in life support CES: Development of soil organic substrate
Колич.характеристики :6 с
Место публикации : LIFE SCIENCES: LIFE SUPPORT SYSTEMS STUDIES-I. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON PRESS LTD, 1997. - Vol. 20: F4.6, F4.8, F4.2 and F4.9 Symposia of COSPAR Scientific Commission F on Life Sciences - Life Support System Studies-I, at the 31st COSPAR Scientific Assembly (JUL 14-SEP 21, 1996, BIRMINGHAM, ENGLAND), Is. 10. - P1827-1832. - ISBN 0273-1177, DOI 10.1016/S0273-1177(97)00848-X. - ISBN 0-08-043307-3
Примечания : Cited References: 13
Аннотация: An experimental model of matter turnover in the biotic cycle: plants (plant biomass) -- mushrooms (residual substrate + mushroom fruit bodies) -- worms (biohumus) -- microorganisms (soil-like substrate) -- plants is presented. The initial mass of soillike substrate was produced from wheat plants grown in a hydroponic system, Three cycles of matter turnover in the biotic cycle were carried out. Grain productivity on soillike substrate was 21.87 g/m(2) day(1). The results obtained were used for designing a CES containing man, plants, soillike substrate, bioregeneration module and aquaculture. It was shown, that the circulating dry mass of the CES is 756 kg. The main part (88%) of the circulating mass accumulates in the soillike substrate and bioregeneration module. (C) 1997 COSPAR. Published by Elsevier Science Ltd.
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9.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Manukovsky N.S., Kovalev V.S., Gribovskaya I.V.
Заглавие : Two-stage biohumus production from inedible potato biomass
Колич.характеристики :3 с
Место публикации : Bioresour. Technol.: ELSEVIER SCI LTD, 2001. - Vol. 78, Is. 3. - P273-275. - ISSN 0960-8524, DOI 10.1016/S0960-8524(01)00022-0
Примечания : Cited References: 5
Ключевые слова (''Своб.индексиров.''): inedible potato biomass--wheat straw--bioconversion--residual substrate--biohumus
Аннотация: The feasibility of a two-stage bioconversion of inedible potato biomass into biohumus by oyster mushroom followed by worms was tested. As a raw material for biohumus production the inedible potato biomass in certain properties ranked below wheat straw. The most feasible method to convert the potato wastes into biohumus was to mix them with wheat straw at the mass ratio of 1:3 and then treat with mushrooms followed by worms. This gave a good yield of mushrooms. The biohumus produced from the mixture was suitable for use as a plant growth medium. (C) 2001 Elsevier Science Ltd. All rights reserved.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tikhomirov A.A., Ushakova S.A., Manukovsky N.S., Lisovsky G.M., Kudenko Y.A., Kovalev V.S., Gribovskaya I.V., Tirranen L.S., Zolotukhin I.G., Gros J.B., Lasseur C...
Заглавие : Synthesis of biomass and utilization of plants wastes in a physical model of biological life-support system
Колич.характеристики :9 с
Место публикации : Acta Astronaut.: PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 53: 53rd Congress of the International-Astronautical-Federation (IAF) (OCT 10, 2002, HOUSTON, TEXAS), Is. 04.10.2013. - P249-257. - ISSN 0094-5765, DOI 10.1016/S0094-5765(03)00137-1
Примечания : Cited References: 16
Аннотация: The paper considers problems of biosynthesis of higher plants' biomass and "bioloaical incineration" of plant wastes in a working physical model of biological LSS. The plant wastes are "biologically incinerated" in a special heterotrophic block involving Californian worms, mushrooms and straw. The block processes plant wastes (straw, haulms) to produce soil-like substrate (SLS) on which plants (wheat, radish) are grown. Gas exchange in such a system consists of respiratory gas exchange of SLS and photosynthesis and respiration of plants. Specifics of gas exchange dynamics of high plants - SLS complex has been considered. Relationship between such a gas exchange and PAR irradiance and age of plants has been established. Nitrogen and iron were found to the first to limit plants' growth on SLS when process conditions are deranged. The SLS microflora has been found to have different kinds of ammonifying and denitrifying bacteria which is indicative of intensive transformation of nitrogen-containing compounds. The number of physiological groups of microorganisms in SLS was, on the whole, steady. As a result, organic substances - products of exchange of plants and microorganisms were not accumulated in the medium, but mineralized and assimilated by the biocenosis. Experiments showed that the developed model of a manmade ecosystem realized complete utilization of plant wastes and involved them into the intrasystem turnover. (C) 2003 International Astronautical Federation. Published by Elsevier Science Ltd. All rights reserved.
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11.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Tikhomirov A.A., Ushakova S.A., Manukovsky N.S., Lisovsky G.M., Kudenko Y.A., Kovalev V.S., Gubanov V.G., Barkhatov Y.V., Gribovskaya I.V., Zolotukhin I.G., Gros J.B., Lasseur C...
Заглавие : Mass exchange in an experimental new-generation life support system model based on biological regeneration of environment
Колич.характеристики :10 с
Место публикации : SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - P1711-1720. - ISBN 0273-1177, DOI 10.1016/S0273-1177(03)00108-X
Примечания : Cited References: 13
Аннотация: An experimental model of a biological life support system was used to evaluate qualitative and quantitative parameters of the internal mass exchange. The photosynthesizing unit included the higher plant component (wheat and radish), and the heterotrophic unit consisted of a soil-like substrate, California worms, mushrooms and microbial microflora. The gas mass exchange involved evolution of oxygen by the photosynthesizing component and its uptake by the heterotroph component along with the formation and maintaining of the SLS structure, growth of mushrooms and California worms, human respiration, and some other processes. Human presence in the system in the form of "virtual human" that at regular intervals took part in the respirative gas exchange during the experiment. Experimental data demonstrated good oxygen/carbon dioxide balance, and the closure of the cycles of these gases was almost complete. The water cycle was nearly 100% closed. The main components in the water mass exchange were transpiration water and the watering solution with mineral elements. Human consumption of the edible plant biomass (grains and roots) was simulated by processing these products by a unique physicochemical method of oxidizing them to inorganic mineral compounds, which were then returned into the system and fully assimilated by the plants. The oxidation was achieved by "wet combustion" of organic biomass, using hydrogen peroxide following a special procedure, which does not require high temperature and pressure. Hydrogen peroxide is produced from the water inside the system. The closure of the cycle was estimated for individual elements and compounds. Stoichiometric proportions are given for the main components included in the experimental model of the system. Approaches to the mathematical modeling of the cycling processes are discussed, using the data of the experimental model. Nitrogen, as a representative of biogenic elements, shows an almost 100% closure of the cycle inside the system, The proposed experimental model of a biological system is discussed as a candidate for potential application in the investigations aimed at creating ecosystems with largely closed cycles of the internal mass exchange. The formation and maintenance of sustainable cycling of vitally important chemical elements and compounds in biological life support systems (BLSS) is an extremely pressing problem. To attain the stable functioning of biological life support systems (BLSS) and to maintain a high degree of closure of material cycles in them, it is essential to understand the character of mass exchange processes and stoichiometric proportions of the initial and synthesized components of the system. (C) 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Markova S.V., Vysotski E.S.
Заглавие : Coelenterazine-dependent luciferases
Место публикации : Biochemistry Moscow. - 2015. - Vol. 80, Is. 6. - С. 714-732. - ISSN 00062979 (ISSN) , DOI 10.1134/S0006297915060073
Ключевые слова (''Своб.индексиров.''): bioluminescence--coelenterazine--luciferase--luciferin--coelenterata--cypridina luciferin--fungi--hexapoda--mollusca--protozoa
Аннотация: Bioluminescence is a widespread natural phenomenon. Luminous organisms are found among bacteria, fungi, protozoa, coelenterates, worms, molluscs, insects, and fish. Studies on bioluminescent systems of various organisms have revealed an interesting feature - the mechanisms underlying visible light emission are considerably different in representatives of different taxa despite the same final result of this biochemical process. Among the several substrates of bioluminescent reactions identified in marine luminous organisms, the most commonly used are imidazopyrazinone derivatives such as coelenterazine and Cypridina luciferin. Although the substrate used is the same, bioluminescent proteins that catalyze light emitting reactions in taxonomically remote luminous organisms do not show similarity either in amino acid sequences or in spatial structures. In this review, we consider luciferases of various luminous organisms that use coelenterazine or Cypridina luciferin as a substrate, as well as modifications of these proteins that improve their physicochemical and bioluminescent properties and therefore their applicability in bioluminescence imaging in vivo. © 2015 Pleiades Publishing, Ltd.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev, M., I
Заглавие : Terrestrial Sources of Polyunsaturated Fatty Acids for Aquaculture
Колич.характеристики :14 с
Коллективы : Russian FederationRussian Federation [51.1.1, FSRZ-20200006]
Место публикации : J. Ichthyol.: PLEIADES PUBLISHING INC, 2021. - Vol. 61, Is. 4. - С. 632-645. - ISSN 0032-9452, DOI 10.1134/S0032945221030036. - ISSN 1555-6425(eISSN)
Примечания : Cited References:116. - This work was supported by a state assignment within the framework of the program of fundamental research of the Russian Federation, theme 51.1.1 and FSRZ-20200006.
Предметные рубрики: FISH-OIL REPLACEMENT
RAINBOW-TROUT DIETS
ATLANTIC SALMON
Аннотация: The review considers probable ways to overcome the deficiency of eicosapentaenoic and docosahexaenoic acids in the human diet through the rational development of aquaculture. Currently, aquaculture is not a producer, but a consumer of polyunsaturated fatty acids, since it is based on feed, the main components of which are fishmeal and fish oil obtained from commercial catches. It is proposed to transform farmed fish from a consumer to producer of these polyunsaturated fatty acids. The source of production of eicosapentaenoic and docosahexaenoic acids in aquaculture can be their synthesis by fish from short-chain alpha-linolenic acid contained in the oil of terrestrial agricultural plants. Calculations of the threshold value of the content of eicosapentaenoic and docosahexaenoic acids in feed for salmon fish (Salmonidae) with partial replacement of fish oil with vegetable oil are presented. Another possible way to transform aquaculture into a producer of polyunsaturated fatty acids is to partially replace fishmeal in feed with flour from terrestrial invertebrates. Some species of insects and worms that are promising for aquaculture as objects for the production of feed meal are considered.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Purtov, K., V, Petushkov V. N., Rodionova N. S., Chepurnykh, T., V, Kozhemyako V. B., Zagitova, R., I, Shcheglov A. S., Ziganshin R. H., Tsarkova A. S.
Заглавие : SIMILARITIES AND DIFFERENCES BETWEEN THE CHAETOPTERUS VARIOPEDATUS POLYCHAETE LUCIFERASES DEPENDING ON THE TYPE OF HABITAT
Колич.характеристики :6 с
Место публикации : Bull. Russ. State Med. Univ.: PIROGOV RUSSIAN NATL RESEARCH MEDICAL UNIV, 2021. - Is. 5. - С. 41-46. - ISSN 2500-1094, DOI 10.24075/vrgmu.2021.049. - ISSN 2542-1204(eISSN)
Примечания : Cited References:20
Предметные рубрики: BIOLUMINESCENCE
ANNELIDA
SYSTEM
Аннотация: The marine polychaete Chaetopterus variopedatus (Renier) (family Chaetopteridae) is a cosmopolitan species complex, consisting of distinct populations/subspecies. The worms release glowing (460 nm) clouds of mucus when disturbed, and their parapodia often glow brightly. Currently, it is still unclear how exactly the bioluminescence system of these polychaetes functions. It has been previously assumed that the C. variopedatus luciferase may be used for detection of ferroptosis, the recently explored pathway of programmed cell death, resulting from accumulation of the ferrous ions. This study was aimed to extract and characterize the C. variopedatus luciferases, as well as to compare luciferases obtained from C. variopedatus of different populations. When extracting the enzyme responsible for bioluminescence from the frozen samples of Brazilian C. variopedatus using the improved method, two active luciferases, L1 and L2, were obtained. We assumed that one of the listed above luciferases was responsible for luminescence of the mucus and the other luciferase was responsible for luminescence in parapodia, and used the method for the distinct samples of mucus and parapodia of the living Far Eastern C. variopedatus. However, mucus of the latter turned out to be non-glowing. It is shown that luciferase L2 is responsible for luminescence in the parapodia of the C. variopedatus polychaete, since this luciferase has been found in the total biomass of Brazilian polychaetes and parapodia of Far Eastern polychaetes. Luminescence of the Brazilian C. variopedatus mucus is attributed to the functioning of luciferase L1, which is lacking in the mucus of the Far Eastern subspecies. The range of luciferase isoforms in polychaetes C. variopedatus depends on the place of origin.
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