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Stable isotope fractionation of fatty acids of Daphnia fed laboratory cultures of microalgae / M. I. Gladyshev [et al.] // Limnologica. - 2016. - Vol. 56. - P23-29, DOI 10.1016/j.limno.2015.12.001 . - ISSN 0075-9511
Кл.слова (ненормированные):
Compound-specific isotope analysis -- Fatty acids -- Food webs -- Stable isotope fractionation -- comparative study -- crustacean -- fatty acid -- food web -- isotopic analysis -- isotopic fractionation -- microalga -- stable isotope -- Chlorella vulgaris -- Cryptomonas sp. -- Daphnia -- Daphnia galeata
Аннотация: We tested a comparatively new method of tracing of natural food webs, compound-specific isotope analysis (CSIA) of fatty acids (FA), using laboratory culture of Daphnia galeata fed Chlorella vulgaris and Cryptomonas sp. In general, Daphnia had significantly lighter carbon stable isotope composition of most fatty acids, including essential, than those of their food, microalgae. Thus, our results did not support the pivotal premise of the FA-CSIA application for food web analysis, i.e., transmitting the isotope 'signal' of essential FAs to consumers from their food without any modification. Moreover, the values of isotope fractionation of particular FAs in the consumer relative to its food were not constant, but varied from 1.35‰ to 7.04‰. The different isotope fractionation (depletion) values of diverse FAs in consumer were probably caused by different processes of their synthesis, catabolism and assimilation. More work is evidently to be done for correct interpretation of results of FA-CSIA during field studies for tracing of natural food webs. © 2015 Elsevier GmbH.

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Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation
Siberian Federal University, Svobodny av. 79, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Gladyshev, M. I.; Makhutova, O. N.; Kravchuk, E. S.; Anishchenko, O. V.; Sushchik, N. N.

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Mercury, selenium and fish oils in marine food webs and implications for human health / M. O. Gribble [et al.] // J. Mar. Biol. Assoc. U.K. - 2016. - Vol. 96, Is. 1. - P43-59, DOI 10.1017/S0025315415001356 . - ISSN 0025-3154
Кл.слова (ненормированные):
Docosahexaenoic acid -- Ecotoxicology -- Eicosapentaenoic acid -- Fish oils -- Mercury -- N-3 fatty acids -- Oceans and human health -- OHH -- Public health -- Selenium
Аннотация: Humans who eat fish are exposed to mixtures of healthful nutrients and harmful contaminants that are influenced by environmental and ecological factors. Marine fisheries are composed of a multitude of species with varying life histories, and harvested in oceans, coastal waters and estuaries where environmental and ecological conditions determine fish exposure to both nutrients and contaminants. Many of these nutrients and contaminants are thought to influence similar health outcomes (i.e., neurological, cardiovascular, immunological systems). Therefore, our understanding of the risks and benefits of consuming seafood require balanced assessments of contaminants and nutrients found in fish and shellfish. In this paper, we review some of the reported benefits of fish consumption with a focus on the potential hazards of mercury exposure, and compare the environmental variability of fish oils, selenium and mercury in fish. A major scientific gap identified is that fish tissue concentrations are rarely measured for both contaminants and nutrients across a range of species and geographic regions. Interpreting the implications of seafood for human health will require a better understanding of these multiple exposures, particularly as environmental conditions in the oceans change. © Marine Biological Association of the United Kingdom, 2015.

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Держатели документа:
Department of Preventive Medicine, University of Southern California, Keck School of Medicine, Los Angeles, CA, United States
School of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, NY, United States
Department of Environmental Health Sciences, University at Albany School of Public Health, State University of New York, Rensselaer, NY, United States
Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC, United States
Department of Veterinary Medicine, College of Natural Science and Mathematics, University of Alaska Fairbanks, Fairbanks, AK, United States
Institute of Biophysics of Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Department of Biological Sciences, Dartmouth College, Hanover, NH, United States

Доп.точки доступа:
Gribble, M. O.; Karimi, R.; Feingold, B. J.; Nyland, J. F.; O'Hara, T. M.; Gladyshev, M. I.; Chen, C. Y.

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Creation of Artificial Luciferases to Expand their Analytical Potential [Text] / L. A. Frank // Comb. Chem. High Throughput Screen. - 2015. - Vol. 18, Is. 10. - P919-929, DOI 10.2174/1386207318666150917100011. - Cited References:79. - The work was supported by: the RFBR grant No. 14-08-00902/14; the State budget allocated to the fundamental research at the Russian Academy of Sciences (project No. VI 57.1.1). . - ISSN 1386-2073. - ISSN 1875-5402

РУБ Biochemical Research Methods + Chemistry, Applied + Pharmacology &
Рубрики:
BIOLUMINESCENT REPORTER APPLICATIONS
COELENTERAZINE-BINDING PROTEIN
Кл.слова (ненормированные):
Luciferase -- luciferin -- photoprotein -- bioluminescence -- mutagenesis -- luciferase-based assay -- bioimaging -- reporter assay
Аннотация: Bioluminescent proteins have been intensively used as high sensitive reporters in all kinds of binding assays (immuno-, nucleic acid hybridization assays, etc.) and in bioimaging. But natural luciferases do not always meet the requirements set for them as the assay reporters: thermostabitity, definite bioluminescence spectral and kinetics characteristics, stability to chemical modifications, etc. Luciferases with different appropriate characteristics as well as various luciferin derivatives were obtained using mutagenesis and chemical synthesis. Thanks to rigorous efforts of many researchers bioluminescence-based analytical techniques offer a great potential for solving analytical tasks in the field of biotechnology, biomedicine, pharmacology, etc.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Photobiol Lab, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Frank, Ludmila A.; RFBR [14-08-00902/14]; [VI 57.1.1]

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A bioluminescent assay for detecting melanocortin-1 receptor (MC1R) gene polymorphisms R160W, R151C, and D294H / E. E. Bashmakova [et al.] // Mol. Biol. - 2015. - Vol. 49, Is. 6. - P852-857, DOI 10.1134/S0026893315050039 . - ISSN 0026-8933
Кл.слова (ненормированные):
bioluminescent assay -- MC1R receptor -- melanoma -- single nucleotide polymorphisms
Аннотация: Several polymorphisms in the melanocortin-1 receptor gene (MC1R) have been associated with melanoma risk. In particular, rs1805007, rs1805008, and rs1805009 mutations, which result in R151C, R160W, and D294H amino acid substitutions, respectively, and are associated with the phenotype of red-hair mutations, have also been connected with melanoma and nonmelanoma skin cancer risks. This work describes a method of detecting these polymorphisms using primer extension with subsequent dual bioluminescent assay. Model plasmids carrying polymorphic MC1R fragments, as well as several clinical DNA samples, were tested using the proposed technique. The results agreed well with those obtained by Sanger sequencing. © 2015, Pleiades Publishing, Inc.

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Держатели документа:
Siberian Federal University, Krasnoyarsk, Russian Federation
Blokhin Cancer Research Center, Moscow, Russian Federation
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation
Voino-Yasenetskii Krasnoyarsk State Medical University, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Bashmakova, E. E.; Krasitskaya, V. V.; Bondar, A. A.; Kozlova, A. V.; Ruksha, T. G.; Frank, L. A.
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Creation of artificial luciferases to expand their analytical potential / L. A. Frank // Comb. Chem. High Throughput Screen. - 2015. - Vol. 18, Is. 10. - P919-929 . - ISSN 1386-2073
Кл.слова (ненормированные):
Bioimaging -- Bioluminescence -- Luciferase -- Luciferase-based assay -- Luciferin -- Mutagenesis -- Photoprotein -- Reporter assay
Аннотация: Bioluminescent proteins have been intensively used as high sensitive reporters in all kinds of binding assays (immuno-, nucleic acid hybridization assays, etc.) and in bioimaging. But natural luciferases do not always meet the requirements set for them as the assay reporters: thermostabitity, definite bioluminescence spectral and kinetics characteristics, stability to chemical modifications, etc. Luciferases with different appropriate characteristics as well as various luciferin derivatives were obtained using mutagenesis and chemical synthesis. Thanks to rigorous efforts of many researchers bioluminescencebased analytical techniques offer a great potential for solving analytical tasks in the field of biotechnology, biomedicine, pharmacology, etc. © 2015 Bentham Science Publishers.

Scopus
Держатели документа:
Photobiology Laboratory, Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russian Federation
Siberian Federal University, Svobodnii ave., 79, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Frank, L. A.

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Mitrocomin from the jellyfish Mitrocoma cellularia with deleted C-terminal tyrosine reveals a higher bioluminescence activity compared to wild type photoprotein / L. P. Burakova [et al.] // J. Photochem. Photobiol. B Biol. - 2016. - Vol. 162. - P286-297, DOI 10.1016/j.jphotobiol.2016.06.054 . - ISSN 1011-1344
Аннотация: The full-length cDNA genes encoding five new isoforms of Ca2 +-regulated photoprotein mitrocomin from a small tissue sample of the outer bell margin containing photocytes of only one specimen of the luminous jellyfish Mitrocoma cellularia were cloned, sequenced, and characterized after their expression in Escherichia coli and subsequent purification. The analysis of cDNA nucleotide sequences encoding mitrocomin isoforms allowed suggestion that two isoforms might be the products of two allelic genes differing in one amino acid residue (64R/Q) whereas other isotypes appear as a result of transcriptional mutations. In addition, the crystal structure of mitrocomin was determined at 1.30 A resolution which expectedly revealed a high similarity with the structures of other hydromedusan photoproteins. Although mitrocomin isoforms reveal a high degree of identity of amino acid sequences, they vary in specific bioluminescence activities. At that, all isotypes displayed the identical bioluminescence spectra (473–474 nm with no shoulder at 400 nm). Fluorescence spectra of Ca2 +-discharged mitrocomins were almost identical to their light emission spectra similar to the case of Ca2 +-discharged aequorin, but different from Ca2 +-discharged obelins and clytin which fluorescence is red-shifted by 25–30 nm from bioluminescence spectra. The main distinction of mitrocomin from other hydromedusan photoproteins is an additional Tyr at the C-terminus. Using site-directed mutagenesis, we showed that this Tyr is not important for bioluminescence because its deletion even increases specific activity and efficiency of apo-mitrocomin conversion into active photoprotein, in contrast to C-terminal Pro of other photoproteins. Since genes in a population generally exist as different isoforms, it makes us anticipate the cloning of even more isoforms of mitrocomin and other hydromedusan photoproteins with different bioluminescence properties. © 2016 Elsevier B.V.

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Держатели документа:
Photobiology Laboratory, Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russian Federation
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
Institute of Molecular and Clinical Medicine, Kunming Medical University, Kunming, China
iHuman Institute, ShanghaiTech University, Shanghai, China

Доп.точки доступа:
Burakova, L. P.; Natashin, P. V.; Markova, S. V.; Eremeeva, E. V.; Malikova, N. P.; Cheng, C.; Liu, Z. -J.; Vysotski, E. S.

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Secondary Production of Highly Unsaturated Fatty Acids by Zoobenthos Across Rivers Contrasting in Temperature [Text] / M. I. Gladyshev [et al.] // River Res. Appl. - 2016. - Vol. 32, Is. 6. - P1252-1263, DOI 10.1002/rra.2945. - Cited References:55. - The work was supported by the project no. 6.1089.214/K of Siberian Federal University carried out according to Federal Tasks of Ministry of Education and Science of Russian Federation and by Russian Federal Tasks of Fundamental Research (project no. 51.1.1). . - ISSN 1535-1459. - ISSN 1535-1467

РУБ Environmental Sciences + Water Resources
Рубрики:
AQUATIC ECOSYSTEMS
   CLIMATE-CHANGE
   YENISEI RIVER
   ALPINE PONDS
   LAKE
Кл.слова (ненормированные):
essential polyunsaturated fatty acids -- river zoobenthos -- secondary -- production -- biodiversity -- water temperature -- climate warming
Аннотация: Highly unsaturated fatty acids (HUFA), namely eicosapentaenoic acid (20:5n-3, EPA) and docosahexaenoic acid (22:6n-3, DHA), which are essential for many animals, including humans, are mainly produced in aquatic trophic webs. In fast-flowing rivers, macrozoobenthos is the main source of HUFA for fish and may be particularly vulnerable to thermal alterations associated with climate change. We studied benthic communities in a unique natural ecosystem: the Yenisei River downstream of the dam of Krasnoyarsk Hydroelectric Power Station with very low temperature in summer because of discharge of cold water from deep in the reservoir and its tributaries with high summer temperature. This natural experiment' allowed to get rid of confounding factors, such as differences in light, seasonality, geology (biogeochemistry) and biogeography (regional species pools). As found, in spite of an increase of biodiversity and rates of daily production in warm rivers compared with cold sites, DHA and partly EPA production of zoobenthos decreased with the increase of temperature because of changes in species composition. Thus, in a climate warming context, we can predict a decrease of production of these HUFA by river zoobenthos and thereby a diminishing of their supply for fish and next to humans. Copyright (c) 2015 John Wiley & Sons, Ltd.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Gladyshev, M. I.; Sushchik, N. N.; Shulepina, S. P.; Ageev, A. V.; Dubovskaya, O. P.; Kolmakova, A. A.; Kalachova, G. S.; Siberian Federal University [6.1089.214/K]; Russian Federal Tasks of Fundamental Research [51.1.1]

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Characteristics of fatty acid composition of Gammarus lacustris inhabiting lakes with and without fish [Text] / O. N. Makhutova [et al.] // Dokl. Biochem. Biophys. - 2016. - Vol. 466, Is. 1. - P20-22, DOI 10.1134/S1607672916010063. - Cited References:11. - This study was supported by the Russian Foundation for Basic Research (project no. 14-05-00286), the state task under the basic research program of the Russian Federation (project no. 51.1.1), and the state task of the Ministry of Education and Science of the Russian Federation to the Siberian Federal University (project no. 6.1089.214/K). . - ISSN 1607-6729. - ISSN 1608-3091

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
DREISSENA
Аннотация: The effect of a biotic factor-the presence of predatory fish in water-on the composition and content of fatty acids in crustaceans was studied in the populations of the lake amphipod Gammarus lacustris from two lakes with fish and three lakes without fish. It was found that, at an overall increase in the quantity and quality of food resources (namely, increase in the content of eicosapentaenoic acid and docosahexaenoic acid (DHA) in the biomass), the relative rate of DHA accumulation in gammarids in the lakes without fish is higher than in the lake with fish.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Siberian Branch, Inst Problems Dev North, Ul Khokhryakova 32-7, Tyumen 229360, Russia.
Siberian Fed Univ, Svobodnyi Pr 79, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Makhutova, O. N.; Sharapova, T. A.; Kalachova, G. S.; Shulepina, S. P.; Gladyshev, M. I.; Russian Foundation for Basic Research [14-05-00286]; state task under the basic research program of the Russian Federation [51.1.1]; state task of the Ministry of Education and Science of the Russian Federation to the Siberian Federal University [6.1089.214/K]

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Role of certain amino acid residues of the coelenterazine-binding cavity in bioluminescence of light-sensitive Ca2+-regulated photoprotein berovin / L. P. Burakova [et al.] // Photochem. Photobiol. Sci. - 2016. - Vol. 15, Is. 5. - P691-704, DOI 10.1039/c6pp00050a . - ISSN 1474-905X
Аннотация: Bright bioluminescence of ctenophores is caused by Ca2+-regulated photoproteins. Although these photoproteins are functionally identical to and share many properties of cnidarian photoproteins, like aequorin and obelin, and retain the same spatial architecture, they are extremely sensitive to light, i.e. lose the ability to bioluminesce on exposure to light over the entire absorption spectrum. In addition, the degree of identity of their amino acid sequences with those of cnidarian photoproteins is only 29.4%. This suggests that the residues involved in bioluminescence of ctenophore and cnidarian photoproteins significantly differ. Here we describe the bioluminescent properties of berovin mutants with substitution of the residues located in the photoprotein internal cavity. Since the spatial structure of berovin bound with a substrate is not determined yet, to identify these residues we have modeled it with an accommodated substrate using the structures of some cnidarian Ca2+-regulated photoproteins with bound coelenterazine or coelenteramide as templates in order to obtain an adequate sampling and to take into account all possible conformers and variants for ligand-protein docking. Based on the impact of substitutions on the bioluminescent properties and model structures we speculate that within the internal cavity of ctenophore photoproteins, coelenterazine is bound as a 2-peroxy anion adduct which is stabilized owing to Coulomb interaction with a positively charged guanidinium group of Arg41 paired with Tyr204. In this case, the bioluminescence reaction is triggered by only calcium-induced conformational changes leading to the disturbance of charge-charge interaction. © 2016 The Royal Society of Chemistry and Owner Societies.

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Держатели документа:
Photobiology Laboratory, Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Burakova, L. P.; Stepanyuk, G. A.; Eremeeva, E. V.; Vysotski, E. S.

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10.

Livers of terrestrial production animals as a source of long-chain polyunsaturated fatty acids for humans: An alternative to fish? [Text] / M. I. Gladyshev [et al.] // Eur. J. Lipid Sci. Technol. - 2015. - Vol. 117, Is. 9. - P1417-1421, DOI 10.1002/ejlt.201400449. - Cited References:41 . - ISSN 1438-7697. - ISSN 1438-9312

РУБ Food Science & Technology + Nutrition & Dietetics
Рубрики:
CARDIOVASCULAR-DISEASE
   DOCOSAHEXAENOIC ACID
   MUSCLE-TISSUE
   MEAT
Кл.слова (ненормированные):
Beef liver -- Chicken liver -- Essential fatty acids -- Pork liver
Аннотация: Long-chain polyunsaturated n-3 fatty acids (LC-PUFA), including eicosapentaenoic (EPA), and docosahexaenoic (DHA) are an essential part of human diets, but their main source, the wild fish catch, is limited. Terrestrial production animals, such as cattle, pigs, and chicken, can synthesize these compounds from the plant-derived precursor -linolenic acid, and the synthesis occurs mainly in liver. We estimated the putative contribution of liver products to the LC-PUFA supply in human nutrition, as an alternative to fish. We measured EPA and DHA contents in raw and cooked livers of the animals. Calculations of global production of EPA and DHA in the livers of these animals were done. Mean contents of LC-PUFA in raw livers of chicken, pigs, and cattle were comparable with those in some fish. Culinary treatments of the liver did not result in a decrease of EPA and DHA contents. Global production of EPA and DHA in animals' livers was estimated as approximate to 410(6)kg/year, while the global EPA+DHA supply through the wild fish catch is known to be 18010(6)kg/year. Thus, liver of production animals is an additional source of LC-PUFA for human nutrition, rather than an alternative to fish. Practical applications: Consumption of food with a high PUFA content and a low n-6/n-3 ratio is recommended for humans to prevent cardiovascular disease and psychiatric disorders. We found that cooked pork and beef liver is a valuable source of n-3 LC-PUFA in human nutrition. In contrast, cooked chicken liver had too high of a n-6/n-3 ratio and, thereby, had a lower nutritive value concerning n-3 LC-PUFA. An estimate has been made of the putative contribution of liver products of terrestrial animals to the LC-PUFA supply as alternative to fish in human nutrition.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.
Krasnoyarsk State Agrarian Univ, Krasnoyarsk, Russia.

Доп.точки доступа:
Gladyshev, Michail I.; Makhutova, Olesia N.; Gubanenko, Galina A.; Rechkina, Ekaterina A.; Kalachova, Galina S.; Sushchik, Nadezhda N.

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11.

Application of Enzyme Bioluminescence for Medical Diagnostics [Text] / L. A. Frank, V. V. Krasitskaya // Adv. Biochem. Eng. Biotechnol. : SPRINGER-VERLAG BERLIN, 2014. - Vol. 144. - P175-197. - (Advances in Biochemical Engineering-Biotechnology), DOI 10.1007/978-3-662-43385-0_6. - Cited References:63 . -

РУБ Biotechnology & Applied Microbiology
Рубрики:
RESONANCE ENERGY-TRANSFER
POLYMERASE-CHAIN-REACTION
LUCIFERASE
Кл.слова (ненормированные):
Bioluminescence -- Ca2+-regulated photoprotein -- Diagnostics -- Immunoassay -- Luciferase -- Nucleic acid hybridization assay
Аннотация: Nowadays luciferases are effectively used as analytical instruments in a great variety of research fields. Of special interest are the studies dealing with elaboration of novel analytical systems for the purposes of medical diagnostics. The ever-expanding spectrum of clinically important analytes accounts for the increasing demand for new techniques for their detection. In this chapter we have made an attempt to summarize the results on applications of luciferases as reporters in binding assays including immunoassay, nucleic acid hybridization assay, and so on. The data over the last 15 years have been analyzed and clearly show that luciferase-based assays, due to extremely high sensitivity, low cost, and the lack of need for skilled personnel, hold much promise for clinical diagnostics.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Photobiol Lab, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
ИБФ СО РАН

Доп.точки доступа:
Frank, Ludmila A.; Krasitskaya, Vasilisa V.

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12.

Fatty acid composition and polyhydroxyalkanoates production by Cupriavidus eutrophus B-10646 cells grown on different carbon sources [Text] / N. Zhila, G. Kalacheva, T. Volova // Process Biochem. - 2015. - Vol. 50, Is. 1. - P69-78, DOI 10.1016/j.procbio.2014.10.018. - Cited References:66. - This study was supported by the Russian Science Foundation (Grant No.14-26-00039). . - ISSN 1359-5113. - ISSN 1873-3298

РУБ Biochemistry & Molecular Biology + Biotechnology & Applied Microbiology
Рубрики:
HYDROGEN-OXIDIZING BACTERIA
CHAIN TRANSFER AGENTS
RALSTONIA-EUTROPHA
Кл.слова (ненормированные):
Cupriavidus eutrophus -- Fatty acid -- Polyhydroxyalkanoates -- Molecular -- weight -- Different substrates
Аннотация: Growth of Cupriavidus eutrophus B-10646, fatty acid (FA) composition of the lipids in the cytoplasmic membrane and cell wall, and polyhydroxyalkanoate (PHA) accumulation by the cells of this new promising producer of PHAs were studied in batch culture, using various carbon sources (fructose, glucose, oleic acid, sunflower seed oil, glycerol, CO2:O-2:H-2). In cells grown on sugars and in autotrophic culture, during the phase of active cell growth, the major FAs of the lipids of cytoplasmic membrane (CMLs) were palmitic, palmitoleic, and cis-vaccenic acids. In the stationary phase of cultivation, cyclopropane FAs were also detected. When carbohydrate substrate was replaced by oleic acid or sunflower seed oil, the proportion of oleic acid in the total FAs of the cytoplasmic membrane increased considerably. In addition to that, the lipid FAs of bacterial cells grown on sunflower seed oil also contained linoleic acid, which is the major acid of sunflower seed oil. The major acids of strongly bound lipids of cell wall (SBLs) were myristic acid and long-chain-length beta-hydroxy acids. Molecular weight of the polymer was measured during its accumulation in cells; for the first time, the presence of 2 polymer fractions (high-molecular weight and low-molecular-weight ones) was recorded in cells with a low polymer content. (C) 2014 Elsevier Ltd. All rights reserved.

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Держатели документа:
SB RAS, Inst Biophys, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
ИБФ СО РАН

Доп.точки доступа:
Zhila, Natalia; Kalacheva, Galina; Volova, Tatiana; Russian Science Foundation [14-26-00039]

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13.

Seasonal dynamics of amino acids in two small Siberian reservoirs dominated by prokaryotic and eukaryotic phytoplankton [Text] / G. S. Kalachova [et al.] // Aquat. Ecol. - 2004. - Vol. 38, Is. 1. - P. 3-15, DOI 10.1023/B:AECO.0000021044.55658.71. - Cited References: 37 . - ISSN 1386-2588

РУБ Ecology + Limnology + Marine & Freshwater Biology
Рубрики:
DISSOLVED ORGANIC-MATTER
   PARTICULATE FATTY-ACIDS
   FRESH-WATER ECOSYSTEMS
   BACTERIAL PRODUCTION
   CHEMICAL-COMPOSITION
   RATES
   MICROALGAE
   PROTEINS
   RELEASE
   DAPHNIA
Кл.слова (ненормированные):
amino acids -- phytoplankton -- cyanobacteria -- blooms
Аннотация: The comparison of the dynamics of phytoplankton biomass and total amino acid composition was made for two water bodies: in one the phytoplankton were dominated by prokaryotes (i.e., there was a bloom of cyanobacteria) and by eukaryotic microalgae in the other. The dynamics of phytoplankton biomass and of total amino acid composition of water were investigated during the vegetation season. It was found that the only factor that significantly changed the percentages of amino acids in water was the bloom of cyanobacteria in the "blooming" water body. During the bloom of cyanobacteria, the absolute and relative content of the Leu-Glu group increased, while the contents of other acids generally dropped. Before and after the bloom, no significant variations in the total amino acid composition were recorded. In the reservoir where eukaryotic microalgae dominated, no significant variations in amino acid composition were recorded during the season.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
Krasnoyarsk State Univ, Krasnoyarsk 660041, Russia
Krasnoyarsk State Agr Univ, Krasnoyarsk 660049, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kalachova, G.S.; Kolmakova, A.A.; Gladyshev, M.I.; Kravchuk, E.S.; Ivanova, E.A.

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14.

Fatty acid composition of Cladocera and Copepoda from lakes of contrasting temperature [Text] / M. I. Gladyshev [et al.] // Freshw. Biol. - 2015. - Vol. 60, Is. 2. - P373-386, DOI 10.1111/fwb.12499. - Cited References:88. - The work was supported by the project No. 6.1089.214/K of SiberianFederal University, carried out according to Federal Tasks of Ministryof Education and Science of Russian Federation, and partly supported bygrant of Russian Foundation for Basic Research (RFBR) No. 14-04-00087.We are grateful to Dr. G. Kirillin, Dr. H.-P. Grossart and Dr. P.Kasprzak for their kind help during sampling at Lake Stechlin and to Dr.M.A. Baturina for valuable assistance at Bolshezemelskaya tundra lakes. . - ISSN 0046-5070. - ISSN 1365-2427

РУБ Marine & Freshwater Biology
Рубрики:
FRESH-WATER ZOOPLANKTON
   SEASONAL DYNAMICS
   DAPHNIA-GALEATA
   TROPHIC
Кл.слова (ненормированные):
climate warming -- essential polyunsaturated fatty acids -- temperature -- adaptation -- zooplankton
Аннотация: We studied the fatty acid (FA) composition of six species of Cladocera and six species of Copepoda from five cold-water lakes, situated in the tundra and/or in the mountains, and eight species of Cladocera and four species of Copepoda from eight warm-water lakes (including one reservoir) in temperate regions. We asked whether the contrasting temperature would result primarily simply in changes in the percentages (i.e. percentage of total FAs) and absolute contents (quantities) of the long-chain polyunsaturated fatty acids (PUFAs), eicosapentaenoic acid (20:5n-3, EPA) and docosahexaenoic acid (22:6n-3, DHA), or whether there are other FAs with various number of double bonds and/or chain lengths which could be responsible for a putative homeoviscous adaptation. We also aimed to reveal any consistent phylogenetic differences in FA percentages and contents between Cladocera and Copepoda, separable from any temperature effects. Both taxa in warm waters had greater percentages of 18:0, and lower percentages of 14:0 and 18:4n-3, than in cold waters, but there were no differences in percentages of DHA. In addition, Cladocera, besides the lower percentage of EPA, had higher percentages of 20:0 and 22:0 in warm waters. These patterns in the percentages of 14:0, 18:0, 18:4n-3, 20:0 and 22:0 are in a good agreement with the hypothesis of homeoviscous adaptation. Thus, the role of EPA, and particularly DHA, as unique regulators of the homeoviscous adaptation of the zooplankton may have been overestimated. Overall, we confirmed the known differences between Cladocera and Copepoda, namely higher percentages of EPA in Cladocera and higher percentages of DHA in Copepoda. However, there was c.50% overlap in the ranges of the percentage of EPA in Cladocera and Copepoda, while the ranges in the content of EPA per unit organic carbon in Cladocera and Copepoda overlapped completely. Differences in the percentages and content of DHA between Cladocera and Copepoda were statistically significant and invariant with temperature, and therefore are probably due to phylogenetic factors, rather than any temperature adaptation. Contrasting temperature was not associated with significant differences in the contents of EPA and DHA per unit of organic carbon within the taxa studied. If this remained the case in a warming climate, such warming would be unlikely to reduce the accumulation of these important PUFAs in the zooplankton, at least if species composition was unchanged. However, if there were shifts in the proportions of Cladocera and Copepoda in the zooplankton, for example fewer copepods as temperature rises, a decrease of the flux of PUFA in the ecosystem is plausible, taking into account the phylogenetic (and temperature invariant) differences in DHA between the two groups.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.
Natl Acad Sci Belarus Bioresources, Sci & Pract Ctr, Minsk, Byelarus.
Russian Acad Sci, Inst Biol, Komi Sci Ctr, Ural Div, Syktyvkar, Russia.
Russian Acad Sci, AN Severtsov Inst Ecol & Evolut, Moscow, Russia.
ИБФ СО РАН

Доп.точки доступа:
Gladyshev, Michail I.; Sushchik, Nadezhda N.; Dubovskaya, Olga P.; Buseva, Zhanna F.; Makhutova, Olesia N.; Fefilova, Elena B.; Feniova, Irina Y.; Semenchenko, Vitaliy P.; Kolmakova, Anzhelika A.; Kalachova, Galina S.; Siberian Federal University [6.1089.214/K]; Russian Foundation for BasicResearch (RFBR) [14-04-00087]

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15.

Novel Peptide Chemistry in Terrestrial Animals: Natural Luciferin Analogues from the Bioluminescent Earthworm Fridericia heliota [Text] / M. A. Dubinnyi [et al.] // Chem.-Eur. J. - 2015. - Vol. 21, Is. 10. - P3942-3947, DOI 10.1002/chem.201406498. - Cited References:17. - We thank Dr. K. V. Antonov for registration of LC-HRMS spectra. This work was supported by the Russian Science Foundation grant 14-50-00131. . - ISSN 0947-6539. - ISSN 1521-3765

РУБ Chemistry, Multidisciplinary
Рубрики:
STRUCTURE ELUCIDATION
DERIVATIVES
IDENTIFICATION
Кл.слова (ненормированные):
bioluminescence -- Fridericia heliota -- luciferin -- peptides -- structure -- elucidation
Аннотация: We report isolation and structure elucidation of AsLn5, AsLn7, AsLn11 and AsLn12: novel luciferin analogs from the bioluminescent earthworm Fridericia heliota. They were found to be highly unusual modified peptides, comprising either of the two tyrosine-derived chromophores, CompX or CompY and a set of amino acids, including threonine, gamma-aminobutyric acid, homoarginine, and unsymmetrical N,N-dimethylarginine. These natural compounds represent a unique peptide chemistry found in terrestrial animals and rise novel questions concerning their biosynthetic origin.

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Scopus
Держатели документа:
Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow, Russian Federation
Pirogov Russian National Research Medical University, Ostrovitianov 1, Moscow, Russian Federation
Laboratory of Photobiology, Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Dubinnyi, Maxim A.; Tsarkova, Aleksandra S.; Petushkov, Valentin N.; Kaskova, Zinaida M.; Rodionova, Natalja S.; Kovalchuk, Sergey I.; Ziganshin, Rustam H.; Baranov, Mikhail S.; Mineev, Konstantin S.; Yampolsky, Ilia V.; Russian Science Foundation [14-50-00131]

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16.

Differences in organic matter and bacterioplankton between sections of the largest Arctic river: Mosaic or continuum? [Text] / M. I. Gladyshev [et al.] // Limnol. Oceanogr. - 2015. - Vol. 60, Is. 4. - P1314-1331, DOI 10.1002/lno.10097. - Cited References:75. - At the stage of laboratory analyses, calculations, and generalizations, the work was supported by the project No. 6.1089.214/K of Siberian Federal University, carried out according to Federal Tasks of Ministry of Education and Science of Russian Federation, and by Russian Federal Tasks of Fundamental Research (project No. 51.1.1). The research cruise was supported by the Attracting Leading Scientists to Russian Educational Institutions Program of the Russian Federation, agreement 11.G34.31.0014. . - ISSN 0024-3590. - ISSN 1939-5590

РУБ Limnology + Oceanography
Рубрики:
FATTY-ACID-COMPOSITION
   KARA SEA
   YENISEI RIVER
   CARBON-CYCLE
Аннотация: We studied biogeochemical characteristics, including organic carbon and nitrogen contents, fatty acid (FA) composition, stable isotope ratios, and primary production in conjunction with species composition of bacterioplankton, using next generation sequencing, in the Yenisei River along a distance similar to 1800km. Basing on FA composition of particulate organic matter (POM) and on other indicators of sources of POM, the river was subdivided into four sections. The upper section 1, situated in mountain region, was the net source of high-quality autochthonous organic matter, produced primarily by diatoms and partly consumed by specialized bacteria species. Section 2 in plain taiga was net sink of high quality allochthonous and autochthonous organic matter, produced by cyanobacteria and green algae. Section 3 was net sink of organic matter, primarily allochthonous, consumed by the specialized species of bacteria. The lowest section 4, situated in tundra, was primarily the conduit of recalcitrant terrestrial organic matter, but also the net source of autochthonous organic matter, produced by diatoms. Biogeochemical traits of sections of the Yenisei River evidently shaped dominant species composition of bacterioplankton of these sections. Regarding the biogeochemical and microbiological data, we concluded that the Yenisei River ecosystem complexly combines features of river mosaic, river continuum, and "neutral pipe."

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Scopus
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Gladyshev, Michail I.; Kolmakova, Olesia V.; Tolomeev, Alexander P.; Anishchenko, Olesia V.; Makhutova, Olesia N.; Kolmakova, Anzhelika A.; Kravchuk, Elena S.; Glushchenko, Larisa A.; Kolmakov, Vladimir I.; Sushchik, Nadezhda N.; Siberian Federal University [6.1089.214/K]; Russian Federal Tasks of Fundamental Research [51.1.1]; Attracting Leading Scientists to Russian Educational Institutions Program of the Russian Federation [11.G34.31.0014]

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17.

Fatty acid and elemental composition of littoral “green tide” algae from the Gulf of Finland, the Baltic Sea / Y. I. Gubelit [et al.] // J. Appl. Phycol. - 2014. - Vol. 27, Is. 1. - P375-386, DOI 10.1007/s10811-014-0349-8 . - ISSN 0921-8971
Кл.слова (ненормированные):
Cladophora glomerata -- Coastal eutrophication -- Essential PUFA -- Green tides -- Ulva intestinalis
Аннотация: Coastal eutrophication leads to a shift in primary producer communities from perennial species to ephemeral fast-growing species of macroalgae, which is known as “green tides” phenomenon. In brackish water habitats of the Baltic Sea, the opportunistic green algae, Cladophora glomerata and Ulva intestinalis, replaced the brown alga Fucus vesiculosus, while in freshwater estuarine parts they replaced epilithic microalgae. We studied Baltic populations of the macroalgae, C. glomerata and U. intestinalis, and epilithic and epiphytic microalgae, with respect to their nutritive quality, such as content of essential polyunsaturated fatty acids (PUFAs) and elemental composition. Fatty acid profiles of the two macroalgae were significantly different, including levels of essential PUFAs. We found a relatively high content of eicosapentaenoic acid (EPA; 20:5n−3) in C. glomerata biomass (4.14 mg g−1 C), whereas U. intestinalis had a lower value (0.45 mg g−1 C). Comparison with literature data showed that C. glomerata appeared to be a more valuable food for potential invertebrate consumers in respect to EPA content and stoichiometric C/P ratio than perennial F. vesiculosus and U. intestinalis. Thus, replacement of F. vesiculosus and epilithic microalgae by C. glomerata would not decrease the potential nutritive value of coastal algal communities for invertebrates. In turn, if U. intestinalis dominate in algal community, the nutritive value of primary producers in coastal zone would decrease. However, in following works, other important indicators of nutritive value, such as sterols and amino acids, should be included in the estimations of green tide algae species. © 2014, Springer Science+Business Media Dordrecht.

Scopus
Держатели документа:
Zoological Institute of the Russian Academy of Science, Universitetskaya emb. 1, Saint Petersburg, Russian Federation
Institute of Biophysics of Siberian Branch of the Russian Academy of Science, Akademgorodok, Krasnoyarsk, Russian Federation
Siberian Federal University, Svobodny av. 79, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Gubelit, Y. I.; Makhutova, O. N.; Sushchik, N. N.; Kolmakova, A. A.; Kalachova, G. S.; Gladyshev, M. I.

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18.

Total synthesis of AsLn2 - A luciferin analogue from the Siberian bioluminescent earthworm Fridericia heliota / A. S. Tsarkova [et al.] // Mendeleev Commun. - 2015. - Vol. 25, Is. 2. - P99-100, DOI 10.1016/j.mencom.2015.03.005 . - ISSN 0959-9436
Аннотация: Total synthesis of AsLn2, a luciferin analogue isolated from the Siberian bioluminescent earthworm F. heliota, was performed from (Z)-5-(2,3-dimethoxy-3-oxoprop-1-en-1-yl)-2-hydroxybenzoic acid in six steps. © 2015 Mendeleev Communications. Published by ELSEVIER B.V.

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Держатели документа:
M. M. Shemyakin-Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesMoscow, Russian Federation
N. I. Pirogov Russian National Research Medical UniversityMoscow, Russian Federation
Institute of Fundamental Biology and Biotechnology, Siberian Federal UniversityKrasnoyarsk, Russian Federation
Institute of Biophysics, Siberian Branch of the Russian Academy of SciencesKrasnoyarsk, Russian Federation
'Drugs Technology' Ltd.Khimki, Moscow Region, Russian Federation
ИБФ СО РАН

Доп.точки доступа:
Tsarkova, A.S.; Dubinnyi, M.A.; Baranov, M.S.; Petushkov, V.N.; Rodionova, N.S.; Zagudaylova, M.B.; Yampolsky, I.V.

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19.

Coelenterazine-dependent luciferases / S. V. Markova, E. S. Vysotski // Biochemistry Moscow. - 2015. - Vol. 80, Is. 6. - P714-732, DOI 10.1134/S0006297915060073 . - ISSN 0006-2979
Кл.слова (ненормированные):
bioluminescence -- coelenterazine -- luciferase -- luciferin -- Coelenterata -- Cypridina luciferin -- Fungi -- Hexapoda -- Mollusca -- Protozoa
Аннотация: Bioluminescence is a widespread natural phenomenon. Luminous organisms are found among bacteria, fungi, protozoa, coelenterates, worms, molluscs, insects, and fish. Studies on bioluminescent systems of various organisms have revealed an interesting feature - the mechanisms underlying visible light emission are considerably different in representatives of different taxa despite the same final result of this biochemical process. Among the several substrates of bioluminescent reactions identified in marine luminous organisms, the most commonly used are imidazopyrazinone derivatives such as coelenterazine and Cypridina luciferin. Although the substrate used is the same, bioluminescent proteins that catalyze light emitting reactions in taxonomically remote luminous organisms do not show similarity either in amino acid sequences or in spatial structures. In this review, we consider luciferases of various luminous organisms that use coelenterazine or Cypridina luciferin as a substrate, as well as modifications of these proteins that improve their physicochemical and bioluminescent properties and therefore their applicability in bioluminescence imaging in vivo. © 2015 Pleiades Publishing, Ltd.

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WOS
Держатели документа:
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Markova, S.V.; Vysotski, E.S.

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20.

Benefit-risk ratio of canned pacific saury (Cololabis saira) intake: Essential fatty acids vs. heavy metals / O. V. Anishchenko [et al.] // Food Chem. Toxicol. - 2017. - Vol. 101. - P8-14, DOI 10.1016/j.fct2016.12.035. - Cited References:51. - The work was partly supported by grant NSh-9249.2016.5 from the President of the Russian Federation. . - ISSN 0278-6915. - ISSN 1873-6351

РУБ Food Science & Technology + Toxicology
Рубрики:
EASTERN MEDITERRANEAN SEA
BREAM SPARUS-AURATA
ESSENTIAL ELEMENTS
Кл.слова (ненормированные):
Polyunsaturated fatty acids (PUFA) -- Benefit-risk ratio -- Heavy metals -- Canned fish
Аннотация: Fatty acid (FA) and element contents were studied in 14 brands of canned (in its own juice and with sunflower oil) saury (Cololabis saira), a popular product of Russian market. Canned saury is a valuable source of essential polyunsaturated FA - eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3). To obtain personal daily dose of EPA + DHA of 1 g for prevention of cardiovascular diseases, neural and inflammatory disorders one needs to intake from 26 to 76 g of canned saury, as was calculated for studied brands. ICP-OES analysis of 24 elements showed that Pb concentration in one brand and Cd content in most of studied saury samples exceeded standards for fish meat established by the European Commission. However, values of hazard quotient, HQEFA, which estimate benefit -risk ratio of fish intake, indicate that canned saury is safe product for human nutrition. (C) 2016 Elsevier Ltd. All rights reserved.

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Смотреть статью
Держатели документа:
Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys SB RAS, Akademgorodok 50-50, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, 79 Svobodny Av, Krasnoyarsk 660041, Russia.
Fed Reg Ctr Standardizat Metrol & Testing Krasnoy, 1a Vavilov Str, Krasnoyarsk 660093, Russia.

Доп.точки доступа:
Anishchenko, O. V.; Sushchik, N. N.; Makhutova, O. N.; Kalachova, G. S.; Gribovskaya, I. V.; Morgun, V. N.; Gladyshev, M. I.; Russian Federation [NSh-9249.2016.5]

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