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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev, Michail I., Makhrov, Alexander A., Baydarov, Ilia V., Safonova, Stanislava S., Golod, Viktor M., Alekseyev, Sergey S., Glushchenko, Larisa A., Rudchenko, Anastasia E., Karpov, Vladimir A., Sushchik, Nadezhda N.
Заглавие : Fatty Acid Composition and Contents of Fish of Genus Salvelinus from Natural Ecosystems and Aquaculture
Колич.характеристики :17 с
Место публикации : Biomolecules: MDPI, 2022. - Vol. 12, Is. 1. - Ст.144. - ISSN 2218-273X(eISSN), DOI 10.3390/biom12010144
Примечания : Cited References:82
Предметные рубрики: ALPINUS SALMONIDAE
MOLECULAR ACTIVITY
SEASONAL DYNAMICS
SYMPATRIC
Аннотация: Fatty acids (FA) of muscle tissue of Salvelinus species and its forms, S. alpinus, S. boganidae, S. drjagini, and S. fontinalis, from six Russian lakes and two aquacultures, were analyzed. Considerable variations in FA compositions and contents were found, including contents of eicosapentaenoic and docosahexaenoic acids (EPA and DHA), which are important indicators of fish nutritive value for humans. As found, contents of EPA+DHA (mg center dot g(-1) wet weight) in muscle tissue of Salvelinus species and forms varied more than tenfold. These differences were supposed to be primarily determined by phylogenetic factors, rather than ecological factors, including food. Two species, S. boganidae and S. drjagini, had the highest EPA+DHA contents in their biomass and thereby could be recommended as promising species for aquaculture to obtain production with especially high nutritive value. Basing on revealed differences in FA composition of wild and farmed fish, levels of 15-17-BFA (branched fatty acids), 18:2NMI (non-methylene interrupted), 20:2NMI, 20:4n-3, and 22:4n-3 fatty acids were recommended for verifying trade label information of fish products on shelves, as the biomarkers to differentiate wild and farmed charr.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova, Tatiana G., Kiselev, Evgeniy G., Demidenko, Alexey V., Zhila, Natalia O., Nemtsev, Ivan V., Lukyanenko, Anna V.
Заглавие : Production and Properties of Microbial Polyhydroxyalkanoates Synthesized from Hydrolysates of Jerusalem Artichoke Tubers and Vegetative Biomass
Колич.характеристики :25 с
Коллективы : Project "Agropreparations of the new generation: a strategy of construction and realization" [075-15-2021-626]; State Assignment of the Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Место публикации : Polymers: MDPI, 2022. - Vol. 14, Is. 1. - Ст.132. - ISSN 2073-4360(eISSN), DOI 10.3390/polym14010132
Примечания : Cited References:93. - This study was financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No. 075-15-2021-626) in accordance with Resolution No. 220 of the Government of the Russian Federation of 9 April 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning" (polymer synthesis, properties), and by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (films production, surface properties).
Предметные рубрики: GLUCOSE-UTILIZING STRAIN
RALSTONIA-EUTROPHA
ASPERGILLUS-NIGER
ACID
Аннотация: One of the major challenges in PHA biotechnology is optimization of biotechnological processes of the entire synthesis, mainly by using new inexpensive carbon substrates. A promising substrate for PHA synthesis may be the sugars extracted from the Jerusalem artichoke. In the present study, hydrolysates of Jerusalem artichoke (JA) tubers and vegetative biomass were produced and used as carbon substrate for PHA synthesis. The hydrolysis procedure (the combination of aqueous extraction and acid hydrolysis, process temperature and duration) influenced the content of reducing substances (RS), monosaccharide contents, and the fructose/glucose ratio. All types of hydrolysates tested as substrates for cultivation of three strains-C. necator B-10646 and R. eutropha B 5786 and B 8562-were suitable for PHA synthesis, producing different biomass concentrations and polymer contents. The most productive process, conducted in 12-L fermenters, was achieved on hydrolysates of JA tubers (X = 66.9 g/L, 82% PHA) and vegetative biomass (55.1 g/L and 62% PHA) produced by aqueous extraction of sugars at 80 degrees C followed by acid hydrolysis at 60 degrees C, using the most productive strain, C. necator B-10646. The effects of JA hydrolysates on physicochemical properties of PHAs were studied for the first time. P(3HB) specimens synthesized from the JA hydrolysates, regardless of the source (tubers or vegetative biomass), hydrolysis conditions, and PHA producing strain employed, exhibited the 100-120 degrees C difference between the T-melt and T-degr, prevailing of the crystalline phase over the amorphous one (C-x between 69 and 75%), and variations in weight average molecular weight (409-480) kDa. Supplementation of the culture medium of C. necator B-10646 grown on JA hydrolysates with potassium valerate and epsilon-caprolactone resulted in the synthesis of P(3HB-co-3HV) and P(3HB-co-4HB) copolymers that had decreased degrees of crystallinity and molecular weights, which influenced the porosity and surface roughness of polymer films prepared from them. The study shows that JA hydrolysates used as carbon source enabled productive synthesis of PHAs, comparable to synthesis from pure sugars. The next step is to scale up PHA synthesis from JA hydrolysates and conduct the feasibility study. The present study contributes to the solution of the critical problem of PHA biotechnology-finding widely available and inexpensive substrates.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev, M., I
Заглавие : Terrestrial Sources of Polyunsaturated Fatty Acids for Aquaculture
Колич.характеристики :14 с
Коллективы : Russian FederationRussian Federation [51.1.1, FSRZ-20200006]
Место публикации : J. Ichthyol.: PLEIADES PUBLISHING INC, 2021. - Vol. 61, Is. 4. - С. 632-645. - ISSN 0032-9452, DOI 10.1134/S0032945221030036. - ISSN 1555-6425(eISSN)
Примечания : Cited References:116. - This work was supported by a state assignment within the framework of the program of fundamental research of the Russian Federation, theme 51.1.1 and FSRZ-20200006.
Предметные рубрики: FISH-OIL REPLACEMENT
RAINBOW-TROUT DIETS
ATLANTIC SALMON
Аннотация: The review considers probable ways to overcome the deficiency of eicosapentaenoic and docosahexaenoic acids in the human diet through the rational development of aquaculture. Currently, aquaculture is not a producer, but a consumer of polyunsaturated fatty acids, since it is based on feed, the main components of which are fishmeal and fish oil obtained from commercial catches. It is proposed to transform farmed fish from a consumer to producer of these polyunsaturated fatty acids. The source of production of eicosapentaenoic and docosahexaenoic acids in aquaculture can be their synthesis by fish from short-chain alpha-linolenic acid contained in the oil of terrestrial agricultural plants. Calculations of the threshold value of the content of eicosapentaenoic and docosahexaenoic acids in feed for salmon fish (Salmonidae) with partial replacement of fish oil with vegetable oil are presented. Another possible way to transform aquaculture into a producer of polyunsaturated fatty acids is to partially replace fishmeal in feed with flour from terrestrial invertebrates. Some species of insects and worms that are promising for aquaculture as objects for the production of feed meal are considered.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zhila N. O., Sapozhnikova K. Yu., Kiselev E. G., Vasiliev A. D., Nemtsev I. V., Shishatskaya E. I., Volova T. G.
Заглавие : Properties of degradable polyhydroxyalkanoates (Phas) synthesized by a new strain, cupriavidus necator ibp/sfu-1, from various carbon sources
Место публикации : Polym.: MDPI, 2021. - Vol. 13, Is. 18. - Ст.3142. - ISSN 20734360 (ISSN), DOI 10.3390/polym13183142
Аннотация: The bacterial strain isolated from soil was identified as Cupriavidus necator IBP/SFU-1 and investigated as a PHA producer. The strain was found to be able to grow and synthesize PHAs under autotrophic conditions and showed a broad organotrophic potential towards different carbon sources: sugars, glycerol, fatty acids, and plant oils. The highest cell concentrations (7–8 g/L) and PHA contents were produced from oleic acid (78%), fructose, glucose, and palm oil (over 80%). The type of the carbon source influenced the PHA chemical composition and properties: when grown on oleic acid, the strain synthesized the P(3HB-co-3HV) copolymer; on plant oils, the P(3HB-co-3HV-co-3HHx) terpolymer, and on the other substrates, the P(3HB) homopolymer. The type of the carbon source influenced molecular-weight properties of PHAs: P(3HB) synthesized under autotrophic growth conditions, from CO2, had the highest number-average (290 ± 15 kDa) and weight-average (850 ± 25 kDa) molecular weights and the lowest polydispersity (2.9 ± 0.2); polymers synthesized from organic carbon sources showed increased polydispersity and reduced molecular weight. The carbon source was not found to affect the degree of crystallinity and thermal properties of the PHAs. The type of the carbon source determined not only PHA composition and molecular weight but also surface microstructure and porosity of the polymer films. The new strain can be recommended as a promising P(3HB) producer from palm oil, oleic acid, and sugars (fructose and glucose) and as a producer of P(3HB-co-3HV) from oleic acid and P(3HB-co-3HV-co-3HHx) from palm oil. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Makhutova, Olesia N., Stoyanov, Kirill N.
Заглавие : Fatty acid content and composition in tissues of Baikal grayling (Thymallus baicalensis), with a special focus on DHA synthesis
Колич.характеристики :19 с
Коллективы : Russian Foundation for Basic Research (RFBR)Russian Foundation for Basic Research (RFBR) [20-04-00594]; Russian FederationRussian Federation [51.1.1]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Место публикации : Aquac. Int.: SPRINGER, 2021. - Article in press. - ISSN 0967-6120, DOI 10.1007/s10499-021-00755-w. - ISSN 1573-143X(eISSN)
Примечания : Cited References:75. - The research was funded by a grant from the Russian Foundation for Basic Research (RFBR) N 20-04-00594, by the state assignment within the framework of the Basic Research Program of the Russian Federation (topic no. 51.1.1) and the state assignment of the Ministry of Science and Higher Education of the Russian Federation to Siberian Federal University in 2020 (Project no. FSRZ-2020-0006 "Biologically active substances in environmental, biotechnological and medical systems").
Предметные рубрики: COD GADUS-MORHUA
DOCOSAHEXAENOIC ACID
SEASONAL DYNAMICS
FISH
Аннотация: Long-chain polyunsaturated fatty acids of n-3 family (n-3 LC PUFAs) are physiologically essential compounds required for normal growth and development of animals, including humans. The ability of fish species to synthesize n-3 LC PUFAs varies significantly across different trophic levels. We have studied fatty acid (FA) content (mg/g of wet weight) and level (% of total FAs) in the brain, liver, heart, intestine, female and male gonads, muscle, and adipose tissues of commercially important wild freshwater Baikal grayling. Additionally, FA content and level of Baikal grayling juveniles have been studied. In all tissues of Baikal grayling, some LC PUFAs, namely, 24:5n-3 and 24:6n-3 (C24 PUFAs), have been found. These FAs are the intermediate products in the synthesis of docosahexaenoic acid (DHA, 22:6n-3) by the Sprecher pathway. The levels of C24 PUFAs in tissues differed significantly: the highest levels of C24 PUFAs were found in adipose tissue and the lowest values in the gonads of females, liver, brain, and head of juveniles. According to the dynamics of DHA and C24 PUFAs, the maximum rate of DHA synthesis is achieved in brain of Baikal grayling, while the lowest rate of DHA synthesis probably occurs in adipose tissue. Although all studied tissues had differences in the number of FAs and their levels, 16:0, 18:1n-9, 16:1n-7, 20:5n-3, and DHA dominated. Male gonads contained an extremely high level of furan FAs - presumably beneficial substances for human health. Additionally, the nutritional value of the tissues of Baikal grayling as a source of n-3 LC PUFAs for humans has been estimated.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Feniova, I. Yu, Sakharova E. G., Gladyshev, M., I, Sushchik N. N., Gorelysheva, Z., I, Karpowicz M.
Заглавие : EFFECTS OF FISH ON THE TRANSFER EFFICIENCY OF CARBON, PUFA AND NUTRIENTS FROM PHYTOPLANKTON TO ZOOPLANKTON UNDER EUTROPHIC CONDITIONS
Колич.характеристики :15 с
Место публикации : Zool. Zhurnal: MAIK NAUKA-INTERPERIODICA PUBL, 2021. - Vol. 100, Is. 2. - С. 194-208. - ISSN 0044-5134, DOI 10.31857/S0044513421020215
Примечания : Cited References:49
Предметные рубрики: FATTY-ACIDS
FOOD QUALITY
FRESH-WATER
ZEBRA MUSSELS
PHOSPHORUS
Аннотация: The efficiency of the transfer of carbon, polyunsaturated fatty acids (PUFA), nitrogen and phosphorus from phytoplankton to zooplankton determines the functioning of the entire ecosystem. However, this parameter depends on environmental conditions. Fish as a very important factor to regulate planktonic communities are very likely to affect the efficiency of the transfer of basic elements and substances from phytoplankton to zoo plankton. In experimental mesocosms filled with water from an eutrophic lake and containing phyto- and zooplankton, we investigated how fish affect both primary and secondary production and the efficiency of transfer of carbon, nitrogen, phosphorus, PUFA and fatty acids (FA) from phytoplankton to zooplankton. Two treatments (control and fish treatment) were repeated in three replicates. The transfer efficiency of substances from phytoplankton to zooplankton was measured as the ratio of secondary to primary production, expressed in liters and per biomass unit in percent. The efficiency expressed per liter characterizes the productivity of the water body, while the efficiency expressed per biomass unit indicates the effectiveness of aquatic species to transfer biologically valuable substances from one trophic level to another. We found that phytoplankton-zooplankton-fish interactions are determined not only by predator-prey relationships, but also are affected by the quality of both phytoplankton and zooplankton, measured as the contents of phosphorus, nitrogen, PUFA and FA in their biomass. We showed that, in the presence of fish, the transfer efficiency of carbon, phosphorus, nitrogen, eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), FA per biomass unit from phytoplankton to zooplankton was increased two-fold, 12.4-fold, 2,5-fold, 12.4-fold, 7.4-fold, and 10-fold, respectively, relative to control. This provides the sustainability of the functioning of the ecosystem under fish pressure. Such a mechanism prevents zooplankton over-exploitation by fish and enables to prolong the food chain.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Makhutova O. N., Stoyanov K. N.
Заглавие : Fatty acid content and composition in tissues of Baikal grayling (Thymallus baicalensis), with a special focus on DHA synthesis
Место публикации : Aquac. Int.: Springer Science and Business Media Deutschland GmbH, 2021. - Article in press. - ISSN 09676120 (ISSN), DOI 10.1007/s10499-021-00755-w
Аннотация: Long-chain polyunsaturated fatty acids of n-3 family (n-3 LC PUFAs) are physiologically essential compounds required for normal growth and development of animals, including humans. The ability of fish species to synthesize n-3 LC PUFAs varies significantly across different trophic levels. We have studied fatty acid (FA) content (mg/g of wet weight) and level (% of total FAs) in the brain, liver, heart, intestine, female and male gonads, muscle, and adipose tissues of commercially important wild freshwater Baikal grayling. Additionally, FA content and level of Baikal grayling juveniles have been studied. In all tissues of Baikal grayling, some LC PUFAs, namely, 24:5n-3 and 24:6n-3 (C24 PUFAs), have been found. These FAs are the intermediate products in the synthesis of docosahexaenoic acid (DHA, 22:6n-3) by the Sprecher pathway. The levels of C24 PUFAs in tissues differed significantly: the highest levels of C24 PUFAs were found in adipose tissue and the lowest values in the gonads of females, liver, brain, and head of juveniles. According to the dynamics of DHA and C24 PUFAs, the maximum rate of DHA synthesis is achieved in brain of Baikal grayling, while the lowest rate of DHA synthesis probably occurs in adipose tissue. Although all studied tissues had differences in the number of FAs and their levels, 16:0, 18:1n-9, 16:1n-7, 20:5n-3, and DHA dominated. Male gonads contained an extremely high level of furan FAs — presumably beneficial substances for human health. Additionally, the nutritional value of the tissues of Baikal grayling as a source of n-3 LC PUFAs for humans has been estimated. © 2021, The Author(s), under exclusive licence to Springer Nature Switzerland AG.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prudnikova S. V., Evgrafova S. Y., Volova T. G.
Заглавие : Metabolic activity of cryogenic soils in the subarctic zone of Siberia towards “green” bioplastics
Место публикации : Chemosphere: Elsevier Ltd, 2021. - Vol. 263. - Ст.128180. - ISSN 00456535 (ISSN), DOI 10.1016/j.chemosphere.2020.128180
Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic – poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9–11 °C, the total biomass of microorganisms at a 10-20-cm depth was 226–234 mg g?1 soil and CO2 production was 20–46 mg g?1 day?1. The total abundance of microscopic fungi varied between (7.4 ± 2.3) ? 103 and (18.3 ± 2.2) ? 103 CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 ± 0.1) ? 106 CFU g?1 soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique – plating of isolates on the mineral agar with polymer as sole carbon source – was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)-degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. © 2020 Elsevier Ltd
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nechipurenko, Yury D., Semyonov, Denis A., Lavrinenko, Igor A., Lagutkin, Denis A., Generalov, Evgenii A., Zaitceva, Anna Y., Matveeva, Olga, V, Yegorov, Yegor E.
Заглавие : The Role of Acidosis in the Pathogenesis of Severe Forms of COVID-19
Колич.характеристики :15 с
Коллективы : Presidium of the Russian Academy of Sciences for Molecular and Cellular Biology; Program of Fundamental Research for State Academies for years 2013-2020 [01201363818]
Место публикации : Biology-Basel: MDPI, 2021. - Vol. 10, Is. 9. - Ст.852. - ISSN 2079-7737(eISSN), DOI 10.3390/biology10090852
Примечания : Cited References:86. - This research was funded by the Presidium of the Russian Academy of Sciences for Molecular and Cellular Biology and the Program of Fundamental Research for State Academies for years 2013-2020, project no. 01201363818.
Предметные рубрики: RESPIRATORY-ACIDOSIS
LACTATE
COAGULATION
GLYCOLYSIS
SECRETION
Аннотация: Simple Summary Recently, several studies have shown that acidosis, which is increased acidity in the blood and other body tissues, is often associated with severe COVID-19. In this article, we look at the mechanisms and consequences of acidosis that can lead to an exacerbation of COVID-19. We want to draw the attention of readers to the threshold values of such disease characteristics as hypoxia and acidosis, which are associated with a sharp deterioration in the patient's condition. Hypoxia and acidosis mutually reinforce each other according to the principle of a vicious cycle (that is, they are involved in a system of positive feedbacks). Elevated blood lactate (lactic acid) levels are associated with poor clinical outcomes in COVID patients. As a practical recommendation, we propose to pay more attention to the prevention of acidosis, including in the early stages of the disease, when the adjustment of homeostasis requires less effort and is less risky. COVID-19 has specific characteristics that distinguish this disease from many other infections. We suggest that the pathogenesis of severe forms of COVID-19 can be associated with acidosis. This review article discusses several mechanisms potentially linking the damaging effects of COVID-19 with acidosis and shows the existence of a vicious cycle between the development of hypoxia and acidosis in COVID-19 patients. At the early stages of the disease, inflammation, difficulty in gas exchange in the lungs and thrombosis collectively contribute to the onset of acidosis. In accordance with the Verigo-Bohr effect, a decrease in blood pH leads to a decrease in oxygen saturation, which contributes to the exacerbation of acidosis and results in a deterioration of the patient's condition. A decrease in pH can also cause conformational changes in the S-protein of the virus and thus lead to a decrease in the affinity and avidity of protective antibodies. Hypoxia and acidosis lead to dysregulation of the immune system and multidirectional pro- and anti-inflammatory reactions, resulting in the development of a "cytokine storm". In this review, we highlight the potential importance of supporting normal blood pH as an approach to COVID-19 therapy.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zhila, Natalia O., Sapozhnikova, Kristina Yu, Kiselev, Evgeniy G., Vasiliev, Alexander D., Nemtsev, Ivan, V, Shishatskaya, Ekaterina, I, Volova, Tatiana G.
Заглавие : Properties of Degradable Polyhydroxyalkanoates (PHAs) Synthesized by a New Strain, Cupriavidus necator IBP/SFU-1, from Various Carbon Sources
Колич.характеристики :19 с
Коллективы : Russian FederationRussian Federation [075-15-2021-626, 220]; State Assignment of the Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Место публикации : Polymers: MDPI, 2021. - Vol. 13, Is. 18. - Ст.3142. - ISSN 2073-4360(eISSN), DOI 10.3390/polym13183142
Примечания : Cited References:78. - This work was financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 075-15-2021-626) in accordance with Resolution No 220 of the Government of the Russian Federation of 9 April 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning" (strain isolation, polymer synthesis and investigation), and by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (study of film properties).
Предметные рубрики: RALSTONIA-EUTROPHA
POLY(3-HYDROXYBUTYRATE) PRODUCTION
PLANT OIL
ACID
Аннотация: The bacterial strain isolated from soil was identified as Cupriavidus necator IBP/SFU-1 and investigated as a PHA producer. The strain was found to be able to grow and synthesize PHAs under autotrophic conditions and showed a broad organotrophic potential towards different carbon sources: sugars, glycerol, fatty acids, and plant oils. The highest cell concentrations (7-8 g/L) and PHA contents were produced from oleic acid (78%), fructose, glucose, and palm oil (over 80%). The type of the carbon source influenced the PHA chemical composition and properties: when grown on oleic acid, the strain synthesized the P(3HB-co-3HV) copolymer; on plant oils, the P(3HB-co-3HV-co-3HHx) terpolymer, and on the other substrates, the P(3HB) homopolymer. The type of the carbon source influenced molecular-weight properties of PHAs: P(3HB) synthesized under autotrophic growth conditions, from CO2, had the highest number-average (290 +/- 15 kDa) and weight-average (850 +/- 25 kDa) molecular weights and the lowest polydispersity (2.9 +/- 0.2); polymers synthesized from organic carbon sources showed increased polydispersity and reduced molecular weight. The carbon source was not found to affect the degree of crystallinity and thermal properties of the PHAs. The type of the carbon source determined not only PHA composition and molecular weight but also surface microstructure and porosity of the polymer films. The new strain can be recommended as a promising P(3HB) producer from palm oil, oleic acid, and sugars (fructose and glucose) and as a producer of P(3HB-co-3HV) from oleic acid and P(3HB-co-3HV-co-3HHx) from palm oil.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gusakov, Vladimir A., Makhutova, Olesia N., Gladyshev, Michail I., Golovatyuk L. V., Zinchenko, Tatiana D.
Заглавие : Ecological Role of Cyprideis torosa and Heterocypris salina (Crustacea, Ostracoda) in Saline Rivers of the Lake Elton Basin: Abundance, Biomass, Production, Fatty Acids
Колич.характеристики :16 с
Коллективы : Russian Foundation for Basic Research (RFBR)Russian Foundation for Basic Research (RFBR) [13-04-00740, 15-04-03341, 17-04-00135]; Russian FederationRussian Federation [51.1.1]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Место публикации : Zool. Stud.: BIODIVERSITY RESEARCH CENTER, ACAD SINICA, 2021. - Vol. 60. - Ст.53. - ISSN 1021-5506, DOI 10.6620/ZS.2021.60-53. - ISSN 1810-522X(eISSN)
Примечания : Cited References:84. - This research was performed in the framework of State Assignments No. 121051100109-1 and AAAA-A17-117112040039-7, and it was also supported by the grants from Russian Foundation for Basic Research (RFBR) (projects numbers 13-04-00740, 15-04-03341, 17-04-00135), State Assignment within the framework of the basic research program of the Russian Federation (topic No. 51.1.1), State Assignment of the Ministry of Science and Higher Education of the Russian Federation to Siberian Federal University in 2020 (project No. FSRZ-2020-0006 "Biologically active substances in environmental, biotechnological and medical systems"). The authors are grateful to Elena Krasova for linguistic check and improvements of the manuscript. We would also like to thank two anonymous reviewers for their constructive comments, recommendations as well as extra corrections of the paper's language and style.
Предметные рубрики: JONES 1850 CRUSTACEA
MACROZOOBENTHIC COMMUNITIES
INVERTEBRATES
Аннотация: Saline rivers are highly productive ecosystems in arid regions. The meiobenthic community (bottom meiofauna) and its dominant representatives are one of the least studied components of these aquatic ecosystems. Ostracods Cyprideis torosa and Heterocypris salina are major consumers among the species of bottom meiofauna in saline rivers flowing into the hyperhaline Lake Elton (Volgograd Region, Russia). We estimated the abundance, biomass and production of C. torosa, the dominant species at the mouth of the polyhaline Chernavka River (average salinity is similar to 30 g l(-1)), and H. salina, the dominant species at the mouth of the mesohaline Bolshaya Samoroda River (similar to 13 g l(-1)), in spring (May) and summer (August). Additionally, we studied the composition and content of fatty acids of the ostracods and their potential food sources (bottom sediments with bacterial-algal mats). We found that the abundance and biomass (wet weight with shells) of C. torosa in the Chernavka River and H. salina in the Bolshaya Samoroda River reached 3.5 x 10(6) ind. m(-2) and 117 g m(-2), and 1.1 x 10(5) ind. m(-2) and 12 g m(-2), respectively. The first species formed on average about 85% of the total abundance and 96% of the total biomass of the meiobenthos, and the second one, about 13% and 31%, respectively. The daily production of C. torosa and H. salina can reach 249 and 36 mg m(-2) ash-free dry weight, respectively. The results indicate that these species may play an important role in the total flow of matter and energy in the studied habitats. Based on the fatty acid (FA) composition of the ostracods and their food sources, it was found that C. torosa mainly consumed diatoms, while H. salina preferred bacteria, cyanobacteria, and green algae. Differences between the species were greater than differences between the bottom sediments from the rivers. It may mean that the ostracods selectively consumed different food items that may be related to the different nutrient requirements of the species. Seasonal changes in the FA compositions of the ostracods were higher than in their food sources (bottom sediments), which also indicates selective feeding of the species.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gusakov V. A., Makhutova O. N., Gladyshev M. I., Golovatyuk L. V., Zinchenko T. D.
Заглавие : Ecological role of cyprideis torosa and heterocypris salina (Crustacea, ostracoda) in saline rivers of the lake elton basin: Abundance, biomass, production, fatty acids
Место публикации : Zool. Stud.: Biodiversity Research Center, Academia Sinica, 2021. - Vol. 60. - С. 60-53. - ISSN 10215506 (ISSN), DOI 10.6620/ZS.2021.60-53
Аннотация: Saline rivers are highly productive ecosystems in arid regions. The meiobenthic community (bottom meiofauna) and its dominant representatives are one of the least studied components of these aquatic ecosystems. Ostracods Cyprideis torosa and Heterocypris salina are major consumers among the species of bottom meiofauna in saline rivers flowing into the hyperhaline Lake Elton (Volgograd Region, Russia). We estimated the abundance, biomass and production of C. torosa, the dominant species at the mouth of the polyhaline Chernavka River (average salinity is ~30 g l-1), and H. salina, the dominant species at the mouth of the mesohaline Bolshaya Samoroda River (~13 g l-1), in spring (May) and summer (August). Additionally, we studied the composition and content of fatty acids of the ostracods and their potential food sources (bottom sediments with bacterial-algal mats). We found that the abundance and biomass (wet weight with shells) of C. torosa in the Chernavka River and H. salina in the Bolshaya Samoroda River reached 3.5 ? 106 ind. m-2 and 117 g m-2, and 1.1 ? 105 ind. m-2 and 12 g m-2, respectively. The first species formed on average about 85% of the total abundance and 96% of the total biomass of the meiobenthos, and the second one, about 13% and 31%, respectively. The daily production of C. torosa and H. salina can reach 249 and 36 mg m-2 ash-free dry weight, respectively. The results indicate that these species may play an important role in the total flow of matter and energy in the studied habitats. Based on the fatty acid (FA) composition of the ostracods and their food sources, it was found that C. torosa mainly consumed diatoms, while H. salina preferred bacteria, cyanobacteria, and green algae. Differences between the species were greater than differences between the bottom sediments from the rivers. It may mean that the ostracods selectively consumed different food items that may be related to the different nutrient requirements of the species. Seasonal changes in the FA compositions of the ostracods were higher than in their food sources (bottom sediments), which also indicates selective feeding of the species. © 2021 Academia Sinica, Taiwan.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Feniova, Irina Yu, Karpowicz, Maciej, Gladyshev, Michail I., Sushchik, Nadezhda N., Petrosyan, Varos G., Sakharova, Ekaterina G., Dzialowski, Andrew R.
Заглавие : Effects of Macrobiota on the Transfer Efficiency of Essential Elements and Fatty Acids From Phytoplankton to Zooplankton Under Eutrophic Conditions
Колич.характеристики :15 с
Коллективы : Polish National Science Centre [2016/21/B/NZ8/00434]; Russian Science FoundationRussian Science Foundation (RSF) [21-14-00123]; Federal Tasks for Institute of Biophysics SB RAS [51.1.1]; Federal Tasks for Siberian Federal University [FSRG-2020-0019]; Polish National Agency for Academic ExchangePolish National Agency for Academic Exchange (NAWA) [PPN/ULM/2020/1/00258/U/DRAFT/00001]
Место публикации : Front. Environ. Sci.: FRONTIERS MEDIA SA, 2021. - Vol. 9. - Ст.739014. - ISSN 2296-665X(eISSN), DOI 10.3389/fenvs.2021.739014
Примечания : Cited References:76. - This experiment was performed with support from the Polish National Science Centre (2016/21/B/NZ8/00434). The statistical analysis and its interpretation was performed with support from the Russian Science Foundation (Grant No. 21-14-00123). Biochemical analyses were performed with support by Federal Tasks for Institute of Biophysics SB RAS No. 51.1.1 and Federal Tasks for Siberian Federal University No. FSRG-2020-0019. The preparation of the manuscript by Feniova I. was supported by the Polish National Agency for Academic Exchange (Agreement No. PPN/ULM/2020/1/00258/U/DRAFT/00001).
Предметные рубрики: FRESH-WATER ZOOPLANKTON
DAPHNIA-LONGISPINA
ZEBRA MUSSELS
Аннотация: The transfer pathways of organic matter and elements from phytoplankton to zooplankton in freshwater ecosystems are important for understanding how aquatic ecosystems function. We conducted a mesocosm experiment to determine how fish and zebra mussels altered the transfer efficiencies of essential substances including carbon (C), polyunsaturated fatty acids (PUFAs), total fatty acids (FAs), phosphorus (P), and nitrogen (N) from phytoplankton to zooplankton. We assessed the transfer efficiencies of the essential substances from phytoplankton to zooplankton as the ratio of their zooplankton production (P) per unit of biomass (B) to that of phytoplankton to exclude grazing or predation effects. We hypothesized that zebra mussels and fish would affect the transfer of materials from phytoplankton to zooplankton by altering the contents of essential elements and FAs in phytoplankton and zooplankton communities and/or due to shifts in the planktonic community structure mediated by grazing and/or predation. Fish increased the transfer efficiencies of eicosapentaenoic acid 20:5 omega-3 (EPA), docosahexaenoic acid 22:6 omega-3 (DHA), and P relative to the control. We speculated that fish weakened the control of zooplankton over algal assemblage by selectively feeding on larger cladocerans such as Daphnia. Therefore, fish can increase the relative proportion of high-quality food for zooplankton, improving food conditions for the available zooplankton. In contrast, zebra mussels reduced the transfer efficiencies of EPA and DHA relative to the control treatment likely due to competition with zooplankton for PUFA-rich food particles. However, zebra mussels did not have any impact on the transfer efficiencies of C, total FAs, N, and P. EPA, DHA, and P were transferred more efficiently than C from phytoplankton to zooplankton, while total FAs, which are commonly used as an energetic source, were transferred as efficiently as C. The enrichment of consumers with the most important substances relative to their basal food sources creates the potential for the successful transport of these substances across aquatic trophic webs./p
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14.

Вид документа : Статья из сборника (выпуск продолж. издания)
Шифр издания :
Автор(ы) : Kiselev E. G., Zhila N. O., Volova T. G.
Заглавие : Biodegradable polymers - Perspectives and applications in agriculture
Место публикации : IOP Conference Series: Earth and Environmental Science: IOP Publishing Ltd, 2021. - Vol. 689: 2020 International Conference on Germany and Russia: Ecosystems Without Borders, EcoSystConfKlgtu 2020 (5 October 2020 through 10 October 2020, ) Conference code: 167944, Is. 1. - Ст.012036. - , DOI 10.1088/1755-1315/689/1/012036
Аннотация: The paper presents a brief overview of the results of the implementation of the project "Agropreparations of the new generation: a strategy of construction and realization". The first part contains the analysis of the growth of the wild-type strain Cupriavidus necator B-10646 (formerly eutrophus) and the synthesis of polyhydroxyalkanoates by this strain on various substrates: glycerol, palm oil, Siberian oil seed, sunflower seed oils, and oleic acid. On refined glycerin, a highly productive process is implemented when scaling up, allowing to obtain 128 ± 11 g / L PHA. Evaluation of oils has shown that palm oil is the best carbon substrate. The second part presents the results of the development of environmentally friendly slow-release pesticide formulations. They are a degradable matrix of poly-3-hydroxybutyrate mixed with natural materials (peat, clay, wood flour), into which a pesticide (metribuzin, tribenuron-methyl, fenoxaprop-P-ethyl, azoxystrobin, epoxiconazole, and tebuconazole) has been. The developed preparations showed high activity against pathogenic fungi and weeds and had a much weaker negative effect on the soil microflora. Studies of the degradation of the developed preparations and the release of pesticides into the soil confirm their effectiveness over a long period of time, up to 90 days. © Published under licence by IOP Publishing Ltd.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Beregovaya K. A., Myshkina N. M., Chepurnykh, T., V, Kotlobay A. A., Purtov, K., V, Petushkov V. N., Rodionova N. S., Yampolsky, I., V
Заглавие : Rational Design and Mutagenesis of Fungal Luciferase from Neonothopanus nambi
Колич.характеристики :4 с
Коллективы : Russian Science FoundationRussian Science Foundation (RSF) [16-14-00052P]; President grant for leading scientific schoolsLeading Scientific Schools Program [NSh-2605.2020.4]
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2021. - Vol. 496, Is. 1. - С. 14-17. - ISSN 1607-6729, DOI 10.1134/S1607672921010026. - ISSN 1608-3091(eISSN)
Примечания : Cited References:12. - This work was supported by the grant from the Russian Science Foundation no. 16-14-00052P, alanine screening was performed by the President grant for leading scientific schools NSh-2605.2020.4.
Аннотация: The recently described bioluminescent system from fungi has great potential for developing highly efficient tools for biomedical research. Luciferase enzyme is one of the most crucial components of this system. The luciferase from Neonothopanus nambi fungus belongs to the novel still undescribed protein family. The structure data for this protein is almost absent. A detailed study of the N. nambi luciferase properties is necessary for the improvement of analytical methods based on the fungal bioluminescent system. Here we present the positions of key amino acid residues and their effect on enzyme function described using bioinformatic and experimental approaches. These results are useful for further fungal luciferase structure determination.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Beregovaya K. A., Myshkina N. M., Chepurnykh T. V., Kotlobay A. A., Purtov K. V., Petushkov V. N., Rodionova N. S., Yampolsky I. V.
Заглавие : Rational Design and Mutagenesis of Fungal Luciferase from Neonothopanus nambi
Место публикации : Doklad. Biochem. Biophys.: Pleiades journals, 2021. - Vol. 496, Is. 1. - С. 14-17. - ISSN 16076729 (ISSN), DOI 10.1134/S1607672921010026
Аннотация: Abstract: The recently described bioluminescent system from fungi has great potential for developing highly efficient tools for biomedical research. Luciferase enzyme is one of the most crucial components of this system. The luciferase from Neonothopanus nambi fungus belongs to the novel still undescribed protein family. The structure data for this protein is almost absent. A detailed study of the N. nambi luciferase properties is necessary for the improvement of analytical methods based on the fungal bioluminescent system. Here we present the positions of key amino acid residues and their effect on enzyme function described using bioinformatic and experimental approaches. These results are useful for further fungal luciferase structure determination. © 2021, Pleiades Publishing, Ltd.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N. N., Borisova E. V., Demina (Vitkovskaya) I. A., Makhutova O. N., Gladyshev M. I.
Заглавие : Differences in Fatty Acid Composition between Orb-Weaver Spiders Inhabiting a Riparian Zone and a Steppe Are Associated with the Consumption of Different Chironomid Taxa
Место публикации : Doklad. Biochem. Biophys.: Pleiades journals, 2021. - Vol. 496, Is. 1. - С. 40-43. - ISSN 16076729 (ISSN), DOI 10.1134/S1607672921010117
Аннотация: Abstract: Amphibiotic insects, chironomids of the genera Glyptotendipes and Chironomus, which emerged from saline Lake Shira, differed in composition and content of fatty acids, including the essential eicosapentaenoic acid (20:5n-3, EPA), and upon flying out they were concentrated in different territories, the riparian zone and remote arid steppe zone, respectively. Potential consumers of chironomids adults, the orb-weaver spiders Larinoidessuspicax, which inhabited both zones, also differed in fatty acid composition. The main difference in their biochemical composition was a significantly higher level of EPA in spiders from the riparian zone that likely to be explained by consumption of the Glyptotendipes adults enriched in this fatty acid and concentrated only within this zone. The higher level of EPA, which is deficient in terrestrial ecosystems, in orb-weaver spiders from the riparian zone of the saline lake may potentially promote a successful survival of the consumers in the arid landscape. © 2021, Pleiades Publishing, Ltd.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N. N., Borisova, E., V, Demina I. A., Makhutova O. N., Gladyshev, M., I
Заглавие : Differences in Fatty Acid Composition between Orb-Weaver Spiders Inhabiting a Riparian Zone and a Steppe Are Associated with the Consumption of Different Chironomid Taxa
Колич.характеристики :4 с
Коллективы : Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [19-34-90099, 20-0400346a]; Russian FederationRussian Federation [51.1.1, FSRZ-2020-0006]
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2021. - Vol. 496, Is. 1. - С. 40-43. - ISSN 1607-6729, DOI 10.1134/S1607672921010117. - ISSN 1608-3091(eISSN)
Примечания : Cited References:13. - This work was supported by the Russian Foundation for Basic Research (project nos. 19-34-90099 and 20-0400346a) and state assignments within the framework of the fundamental research program of the Russian Federation (topics nos. 51.1.1 and FSRZ-2020-0006).
Аннотация: Amphibiotic insects, chironomids of the genera Glyptotendipes and Chironomus, which emerged from saline Lake Shira, differed in composition and content of fatty acids, including the essential eicosapentaenoic acid (20:5n-3, EPA), and upon flying out they were concentrated in different territories, the riparian zone and remote arid steppe zone, respectively. Potential consumers of chironomids adults, the orb-weaver spiders Larinoidessuspicax, which inhabited both zones, also differed in fatty acid composition. The main difference in their biochemical composition was a significantly higher level of EPA in spiders from the riparian zone that likely to be explained by consumption of the Glyptotendipes adults enriched in this fatty acid and concentrated only within this zone. The higher level of EPA, which is deficient in terrestrial ecosystems, in orb-weaver spiders from the riparian zone of the saline lake may potentially promote a successful survival of the consumers in the arid landscape.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Malikova N. P., Eremeeva E. V., Gulnov D. V., Natashin P. V., Nemtseva E. V., Vysotski E. S.
Заглавие : Specific Activities of Hydromedusan Ca2+-Regulated Photoproteins
Место публикации : Photochem. Photobiol.: John Wiley and Sons Inc, 2021. - Article in press. - ISSN 00318655 (ISSN), DOI 10.1111/php.13556
Аннотация: Nowadays the recombinant Ca2+-regulated photoproteins originating from marine luminous organisms are widely applied to monitor calcium transients in living cells due to their ability to emit light on Ca2+ binding. Here we report the specific activities of the recombinant Ca2+-regulated photoproteins—aequorin from Aequorea victoria, obelins from Obelia longissima and Obelia geniculata, clytin from Clytia gregaria and mitrocomin from Mitrocoma cellularia. We demonstrate that along with bioluminescence spectra, kinetics of light signals and sensitivities to calcium, these photoproteins also differ in specific activities and consequently in quantum yields of bioluminescent reactions. The highest specific activities were found for obelins and mitrocomin, whereas those of aequorin and clytin were shown to be lower. To determine the factors influencing the variations in specific activities the fluorescence quantum yields for Ca2+-discharged photoproteins were measured and found to be quite different varying in the range of 0.16–0.36. We propose that distinctions in specific activities may result from different efficiencies of singlet excited state generation and different fluorescence quantum yields of coelenteramide bound within substrate-binding cavity. This in turn may be conditioned by variations in the amino acid environment of the substrate-binding cavities and hydrogen bond distances between key residues and atoms of 2-hydroperoxycoelenterazine. © 2021 American Society for Photobiology
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prokopkin I., Makhutova O., Kravchuk E., Sushchik N., Anishchenko O., Gladyshev M.
Заглавие : Assessing the reliability of quantitative fatty acid signature analysis and compound-specific isotope analysis-based mixing models for trophic studies
Место публикации : Biomolecules: MDPI, 2021. - Vol. 11, Is. 11. - Ст.1590. - ISSN 2218273X (ISSN), DOI 10.3390/biom11111590
Аннотация: The study of the trophic relationships of aquatic animals requires correct estimates of their diets. We compared the quantitative fatty acid signature analysis (QFASA) and the isotope?mixing model IsoError, based on the compound?specific isotope analysis of fatty acids (CSIA?FA), which are potentially effective models for quantitative diet estimations. In a 21?day experiment, Daphnia was fed a mixture of two food items, Chlorella and Cryptomonas, which were supplied in nearly equal proportions. The percentages and isotope values of the FAs of the algal species and Daphnia were measured. The IsoError based on CSIA?FA gave an estimation of algae consumption using only one FA, 18:3n?3. According to this model, the proportion of consumption of Chlorella decreased while the proportion of consumption of Cryptomonas increased during the experiment. The QFASA model was used for two FA subsets—the extended?dietary subset, which included sixteen FAs, and the dietary one, which included nine FAs. According to both subsets, the portion of consumed Chlorella decreased from Day 5 to 10 and then increased at Day 21. The comparison of the two model approaches showed that the QFASA model is a more reliable method to determine the contribution of different food sources to the diet of zooplankton than the CSIA?based mixing model. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
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