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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Barashkov V.A., Gitelzon I.I., Nefedov V.P., Trubachev I.N.
Заглавие : The dynamics of the amino acid composition of the medium in cultivating the isolated organs by controlled perfusion (Russian)
Место публикации : Byulleten Eksperimentalnoi Biologii i Meditsiny. - 1975. - Vol. 80, Is. 7. - С. 36-38. - ISSN 03659615 (ISSN)
Ключевые слова (''Своб.индексиров.''): amino acid--amino acid--dog--in vitro study--kidney--liver--sternum--theoretical study--animal--article--culture medium--metabolism--organ culture--organ preservation--perfusion--tissue preservation--kidney--liver--kidney perfusion--liver perfusion--tissue culture--amino acids--animal--culture media--dogs--english abstract--in vitro--organ culture--organ preservation--perfusion--tissue preservation--kidney--liver
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Barashkov V.A., Gitel'zon I.I., Nefedov V.P., Trubachev I.N.
Заглавие : Dynamics of amino acid composition of the medium in isolated organ culture by the controlled perfusion method
Место публикации : Bulletin of Experimental Biology and Medicine. - 1975. - Vol. 80, Is. 7. - С. 759-761. - ISSN 00074888 (ISSN)
Ключевые слова (''Своб.индексиров.''): amino acid--tissue culture medium--dog--in vitro study--organ culture--organ perfusion--theoretical study
Аннотация: The dynamics of the amino acid composition of the perfusion fluid was investigated during adequate perfusion of isolated dog organs (the thorax and a complex consisting of the thoracic organs, kidneys, and liver). The concentration of amino acids such as histidine, lysine, and alanine in the perfusion fluid 6 h after the beginning of perfusion of the organ complex was higher, whereas that of arginine, serine, aspartic acid, threonine with glutamine, isoleucine, proline, leucine, and valine was much lower than initially. In experiments on the isolated thorax the dynamics of the amino acid composition of the medium was studied during perfusion for 4 h. The concentration of alanine, lysine, and histidine in the medium increased, whereas those of serine, aspartic acid, isoleucine, tyrosine, and phenylalanine decreased.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Belobrov P.I.
Заглавие : Behavior of glutamic acid residue during self arrangement of protein molecules (Russian)
Место публикации : Biofizika. - 1975. - Vol. 20, Is. 1. - С. 23-25. - ISSN 00063029 (ISSN)
Ключевые слова (''Своб.индексиров.''): glutamic acid--protein--in vitro study--theoretical study
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Belobrov P.I.
Заглавие : Behaviour of the residue of glutamic acid on self-organization of protein molecules
Место публикации : Biophysics. - 1975. - Vol. 20, Is. 1. - С. 18-21. - ISSN 00063509 (ISSN)
Ключевые слова (''Своб.индексиров.''): glutamic acid--protein--computer analysis--in vitro study--theoretical study
Аннотация: The method of atom-atom potentials has been used to study the glutamyl dipeptide in ionized (glu-) and non-ionized (glu) states. A calculation has been made with a computer of the conformational maps and the statistical sums of glu and glu-. It was found that on transition of glu glu- fall in the energy exceeds the free energy of initiation of the helix with an increased probability of the helical state of the molecule of glu-. From this it is concluded that glu- may in certain conditions be the embryo of the helix on self-organization of protein. В© 1975.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Barashkov V.A., Gitel'zon I.I., Nefedov V.P., Trubachev I.N.
Заглавие : Dynamics of amino acid composition of the medium during culture of isolated liver and kidneys by the controlled perfusion method
Место публикации : Bulletin of Experimental Biology and Medicine. - 1976. - Vol. 80, Is. 11. - С. 1305-1307. - ISSN 00074888 (ISSN)
Ключевые слова (''Своб.индексиров.''): amino acid--dog--in vitro study--kidney perfusion--liver perfusion--theoretical study
Аннотация: The dynamics of the amino acid composition of the medium was investigated during perfusion of the dog liver and kidney for 6 h with a mixture of autogenous plasma and medium No.199 in the ratio of 2:3. During culture of the kidney for 6 h the histidine concentration in the medium increased by 2.2 times compared with initially, the concentration of glutamic acid by 1.7 times, and of alanine and lysine by 1.6 times, whereas the concentrations of arginine, serine, and aspartic acid fell by 3.3 times and those of glutamine with threonine by 2.5 times. During perfusion of the liver the concentration of glutamic acid rose by 2.9 times, of alanine by 2.3 times, cystine by 2.0 times, and glycine by 1.5 times. The concentration of tyrosine fell by half, and that of phenylalanine and serine by 1.4 times. The arginine concentration fell so quickly during perfusion of the liver that by the second hour after the beginning of perfusion no arginine could be found in the medium. The method of amino acid analysis during organ culture as described can be used as a method of developing and correcting culture media.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Trubachev N.I., Gitel'zon I.I., Kalacheva G.S.
Заглавие : Biochemical composition of several blue green algae and Chlorella
Место публикации : Applied Biochemistry and Microbiology. - 1977. - Vol. 12, Is. 2. - С. 155-161. - ISSN 00036838 (ISSN)
Ключевые слова (''Своб.индексиров.''): fatty acid--lipid--protein--vitamin--alga--blue green alga--chlorella--in vitro study--microorganism--plant--theoretical study
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kalacheva G.S., Vysotsky E.S.
Заглавие : Changes of lipids and their fatty acid composition during batch cultivation of the luminescent bacterium
Место публикации : Mikrobiologiya. - 1984. - Vol. 53, Is. 6. - С. 932-937. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): fatty acid--lipid--bacterium--bioluminescence--nonhuman
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : KALACHEVA G.S., VYSOTSKII E.S.
Заглавие : CHANGE IN LIPIDS AND THEIR FATTY-ACID COMPOSITION IN BATCH CULTURING OF LUMINESCENT BACTERIA
Колич.характеристики :5 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1984. - Vol. 53, Is. 6. - P756-760. - ISSN 0026-2617
Примечания : Cited References: 16
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : KALACHEVA G.S., VYSOTSKII E.S.
Заглавие : CHANGE IN LIPIDS AND THEIR FATTY-ACID COMPOSITION IN BATCH CULTURING OF LUMINESCENT BACTERIA
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1984. - Vol. 53, Is. 6. - С. 756-760. - 5. - ISSN 0026-2617
Примечания : Cited References: 16
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Illarrionov B.A., Blinov V.M., Douchenko A.P., Protopopova M.V., Karginov V.A., Mertvetsov N.P., Gitelson J.I.
Заглавие : Isolation of bioluminescent functions from Photobacterium leiognathi: analysis of luxA, luxB, luxG and neighboring genes
Место публикации : Gene. - 1990. - Vol. 86, Is. 1. - С. 89-94. - ISSN 03781119 (ISSN)
Ключевые слова (''Своб.индексиров.''): bioluminescence--expression in e. coli--luciferase--molecular evolution--nucleotide sequence--protein alignment--recombinant dna--luciferase--amino acid sequence--article--bioluminescence--fungus--gene structure--genetic engineering--heredity--nonhuman--nucleotide sequence--priority journal--vibrionaceae--acyltransferases--amino acid sequence--bacterial proteins--base sequence--cloning, molecular--dna, bacterial--genes, structural, bacterial--luciferase--luminescence--molecular sequence data--operon--photobacterium--restriction mapping--escherichia coli--fungi--photobacterium leiognathi--vibrio harveyi--vibrionaceae
Аннотация: Genes encoding luminescence of Photobacterium leiognathi have been cloned in Escherichia coli. The luminescent clones were readily apparent. Among them, a clone containing a recombinant plasmid with a 13.5-kb insertion was identified. This DNA fragment contained all of the luminescence-encoding genes. The luciferase-encoding genes (lux) in this DNA fragment were localized. We have sequenced a part of the cloned lux region and identified the luxA, luxB and luxG genes encoding the ? and ? subunits of luciferase and a ? protein with an Mr of 26 180, respectively. The analysis of deduced amino acid sequences and comparison with known luciferase sequences from Vibrio harveyi, indicate the common origin of these proteins. В© 1990.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : GITELZON I.I., SANDALOVA T.P.
Заглавие : PROSPECTS FOR APPLICATION OF BIOLUMINESCENCE METHOD IN MEDICINE
Колич.характеристики :5 с
Место публикации : VESTNIK AKADEMII MEDITSINSKIKH NAUK SSSR: IZD VO MEDITSINA, 1990. - Is. 9. - С. 31-35. - ISSN 0002-3027
Примечания : Cited References: 41
Предметные рубрики: AMINO-ACID SEQUENCE
NUCLEOTIDE-SEQUENCE
VIBRIO-HARVEYI
BACTERIAL LUCIFERASE
FIREFLY LUCIFERASE
SUBUNIT
CELLS
GENE
PHOTOPROTEINS
EXPRESSION
Аннотация: Major advances in the development and application of the bioluminescent analysis to detect certain biologically active substances are discussed. The main merit of the method lies in its high sensitivity and specificity along with its simplicity and rapid performance. The available methodologies allow for detection of substances of varying nature: Ca2+, ATP, FMN, NAD(P), long-chain aldehydes, ATP- and NAD(P)-dependent enzymes and their substrates, many xenobiotics and antibiotics, and mutagens. The bioluminescence methodologies may be widely applied in clinical laboratory diagnosis.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kratasyuk V.A., Makurina V.I., Kuznetsov A.M., Kudryasheva N.S., Plotnikova N.B., Medvedeva S.E., Gritsenko I.S., Chernykh V.P.
Заглавие : The effect of succinic acid sulfoderivatives on bacterial luminescence
Место публикации : Prikladnaya Biokhimiya i Mikrobiologiya. - 1991. - Vol. 27, Is. 1. - С. 127-133. - ISSN 05551099 (ISSN)
Ключевые слова (''Своб.индексиров.''): luciferase--n (arylsulfonamido)succinimide--unclassified drug--article--bacterium--bioluminescence--nonhuman--bacteria (microorganisms)
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Raibekas A.A.
Заглавие : Green flavoprotein from P. leiognathi: purification, characterization and identification as the product of the lux G(N) gene
Место публикации : Journal of bioluminescence and chemiluminescence. - 1991. - Vol. 6, Is. 3. - P169-176. - ISSN 08843996 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacterial protein--flavoprotein--amino acid sequence--article--bacterial gene--chemistry--genetics--isolation and purification--luminescence--molecular genetics--molecular weight--photobacterium--amino acid sequence--bacterial proteins--flavoproteins--genes, bacterial--luminescence--molecular sequence data--molecular weight--photobacterium--support, u.s. gov't, p.h.s.
Аннотация: A green flavoprotein (GFP) was isolated and purified to homogeneity from Photobacterium leiognathi, strain 208. GFP is a homodimer of molecular weight 54,000 and contains two molecules of an unusual flavin per molecule of protein. Various biochemical characteristics including isoelectric point, trypsin and chymotrypsin degradation, SDS and temperature influence on subunit dissociation and the dissociation of the flavin chromophore, were investigated. The sequence of 23 N-terminal amino acids was determined and found to be concurrent with the N-terminal amino acid sequence encoded by the lux G(N) gene of P. leiognathi. This fact suggests that GFP is a structural component of the Photobacterium luminescence system.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : SANDALOVA T.P., TYULKOVA N.A.
Заглавие : INACTIVATION OF BACTERIAL LUCIFERASES BY N-ETHYLMALEIMIDE
Колич.характеристики :7 с
Место публикации : Biochem.-Moscow: PLENUM PUBL CORP, 1992. - Vol. 57, Is. 6. - P552-558. - ISSN 0006-2979
Примечания : Cited References: 21
Предметные рубрики: AMINO-ACID SEQUENCE
NUCLEOTIDE-SEQUENCE
REACTIVE SULFHYDRYL
PHOTOBACTERIUM-LEIOGNATHI
VIBRIO-HARVEYI
BIOLUMINESCENCE
SUBUNIT
REGION
GENE
Ключевые слова (''Своб.индексиров.''): luciferase--n-ethylmaleimide
Аннотация: The kinetics of inactivation of luciferases from four species of luminescent bacteria by the thiol reagent N-ethylmaleimide were investigated The dependencies of inactivation on ionic strength differed among the enzymes. Increasing the molarity of the buffer increased the rate of inactivation of all luciferases except that of Vibrio harveyi. Modification of Photobacterium phosphoreum luciferase decreased the maximal intensity of bioluminescence, whereas modification of Photobacterium leiognathi and Vibrio fischeri luciferases in high ionic strength buffers decreased the maximal intensity of bioluminescence and changed the luminescence decay rate constant. High ionic strength apparently alters the conformational states of the luciferases.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Rodicheva E.K., Trubachev I.N., Medvedeva S.E., Egorova O.I. U - Shitova LYu
Заглавие : Growth and luminescence of luminous bacteria promoted by agents of microbial origin.
Место публикации : Journal of bioluminescence and chemiluminescence. - 1993. - Vol. 8, Is. 6. - С. 293-299. - ISSN 08843996 (ISSN)
Ключевые слова (''Своб.индексиров.''): amino acid--carbohydrate--folic acid--luciferase--nitrogen--riboflavin--article--biosynthesis--culture medium--electron microscopy--growth, development and aging--kinetics--luminescence--metabolism--photobacterium--physiology--time--ultrastructure--vibrio--amino acids--carbohydrates--culture media--folic acid--kinetics--luciferase--luminescence--microscopy, electron--nitrogen--photobacterium--riboflavin--time factors--vibrio
Аннотация: The examination of four species of luminous bacteria Photobacterium leiognathi, Photobacterium phosphoreum, Vibrio fischeri and Vibrio harveyi has enabled us to reveal some nutrient medium components effecting growth, luminescence intensity and luciferase synthesis. These agents are nucleic components (nucleotides, nucleotides and amine bases), amino acids and vitamins, which are part of hydrolysates from the biomass of various lithotrophic microorganisms, hydrogen-oxidizing, iron-oxidizing and carboxydobacteria. The effect of promoting agents essentially alters the physiological state and ultrastructure of the cells of luminous bacteria and increases luciferase biosynthesis two- to three-fold compared to a control.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gorban A.N., Mirkes E.M., Popova T.G., Sadovsky M.G.
Заглавие : The comparative redundancy of genes of various organisms and viruses
Место публикации : Genetika. - 1993. - Vol. 29, Is. 9. - P1413-1419. - ISSN 00166758 (ISSN)
Ключевые слова (''Своб.индексиров.''): article--dna content--evolution--genome--nonhuman--restriction mapping--virus--amino acid sequence--comparative study--gene frequency--human--molecular genetics--nucleotide sequence--restriction mapping--virus gene--amino acid sequence--base sequence--comparative study--english abstract--gene frequency--genes, viral--human--molecular sequence data--restriction mapping
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : GLADYSHEV M.I., SUSHCHIK N.N.
Заглавие : EFFECT OF MONOMOLECULAR LAYER OF FATTY-ACID ON TEMPERATURE OF SURFACE-WATER FILM
Колич.характеристики :5 с
Место публикации : Izv. Akad. Nauk. Fiz. Atmos. Okean. Biol.: MEZHDUNARODNAYA KNIGA, 1994. - Vol. 30, Is. 1. - С. 74-78. - ISSN 0002-3515
Примечания : Cited References: 16
Предметные рубрики: GAS-EXCHANGE
Аннотация: Effect of condensed monolayer of stearic acid on temperature of surface water film was investigated using an experimental cell for various ratios of water and air temperatures. When the temperature of air was higher than that of water (positive convective flux), spreading of the acid monolayer provided increasing of temperature of the surface water film. Under inverse relation between the air and the water temperatures (negative convective flux) the temperature of the surface water film was decreased by the acid monolayer. The effect observed could be explained by stabilization of the surface water layer in presence of the acid monolayer. This results in suppression of the convective exchange between the surface film and the bulk of the water.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : VOLOVA T.G., KALACHEVA G.S., KONSTANTINOVA V.M.
Заглавие : ACCUMULATION OF POLYHYDROXYBUTYRATE BY THE AEROBIC CO-OXIDIZING BACTERIUM SELIBERIA-CARBOXYDOHYDROGENA
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1994. - Vol. 63, Is. 2. - С. 109-112. - 4. - ISSN 0026-2617
Примечания : Cited References: 20
Аннотация: The synthesis of polyhydroxybutyrate by the carboxydobacterium Seliberia carboxydohydrogena under various growth conditions was studied. The bacterium was able to accumulate poly-beta-hydroxybutyric acid and a heteropolymer that was a product of copolymerization of hydroxybutyric and hydroxyvaleric acids. Nitrogen and sulfur exerted the strongest effect on polymer accumulation in carboxydobacteria: The maximum polymer concentrations in cells reached 28%. Specimens of polyhydroxybutyrate films were obtained, and their degradability by soil microflora was shown.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N.N., Gladyshev M.I., Kalachova G.S., Guseynova V.E.
Заглавие : Rapid assay of fatty acid composition using a portable high-performance liquid chromatograph for monitoring aquatic ecosystems
Место публикации : Journal of Chromatography A. - 1995. - Vol. 695, Is. 2. - С. 223-228. - ISSN 00219673 (ISSN) , DOI 10.1016/0021-9673(94)01090-2
Ключевые слова (''Своб.индексиров.''): fatty acid--alga--article--culture medium--ecology--high performance liquid chromatography--instrument--methodology--priority journal--ultraviolet spectrophotometry--water analysis
Аннотация: The chromatographic conditions presented allowed the separation of the nitrophenacyl derivatives of standards of eleven free fatty acids (FFA) using a portable high-performance chromatograph, suitable for use aboard a research vessel. A statistically significant linear correlation between UV absorbance and amount of the analytes injected was obtained. The method was tested on FFA from algae cultural media. The method can be used for the ecological monitoring of natural waters.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : SETKOV N.A.
Заглавие : OKADAIC ACID IS AN INHIBITOR OF TYPE-1 AND TYPE 2A PROTEIN PHOSPHATASES STIMULATED DNA-SYNTHESIS IN RESTING CELLS NIH 3T3
Колич.характеристики :5 с
Место публикации : Dokl. Akad. Nauk: MEZHDUNARODNAYA KNIGA, 1995. - Vol. 340, Is. 1. - P114-118. - ISSN 0869-5652
Примечания : Cited References: 15
Предметные рубрики: PHOSPHORYLATION
NUCLEI
FUSION
HETERODIKARYONS
PROLIFERATION
FIBROBLASTS
CYCLIN
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