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Dynamics of amino acid composition of the medium in isolated organ culture by the controlled perfusion method / V. A. Barashkov [et al.] // Bulletin of Experimental Biology and Medicine. - 1975. - Vol. 80, Is. 7. - P759-761 . - ISSN 0007-4888
Кл.слова (ненормированные):
amino acid -- tissue culture medium -- dog -- in vitro study -- organ culture -- organ perfusion -- theoretical study
Аннотация: The dynamics of the amino acid composition of the perfusion fluid was investigated during adequate perfusion of isolated dog organs (the thorax and a complex consisting of the thoracic organs, kidneys, and liver). The concentration of amino acids such as histidine, lysine, and alanine in the perfusion fluid 6 h after the beginning of perfusion of the organ complex was higher, whereas that of arginine, serine, aspartic acid, threonine with glutamine, isoleucine, proline, leucine, and valine was much lower than initially. In experiments on the isolated thorax the dynamics of the amino acid composition of the medium was studied during perfusion for 4 h. The concentration of alanine, lysine, and histidine in the medium increased, whereas those of serine, aspartic acid, isoleucine, tyrosine, and phenylalanine decreased.

Scopus
Держатели документа:
Siberian Div., Dept. Biophys., L.V. Kirenskii Inst. Phys., Acad. Sci. USSR, Krasnoyarsk, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Barashkov, V.A.; Gitel'zon, I.I.; Nefedov, V.P.; Trubachev, I.N.

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The dynamics of the amino acid composition of the medium in cultivating the isolated organs by controlled perfusion (Russian) / V. A. Barashkov [и др.] // Byulleten Eksperimentalnoi Biologii i Meditsiny. - 1975. - Vol. 80, Is. 7. - С. 36-38 . - ISSN 0365-9615
Кл.слова (ненормированные):
amino acid -- amino acid -- dog -- in vitro study -- kidney -- liver -- sternum -- theoretical study -- animal -- article -- culture medium -- metabolism -- organ culture -- organ preservation -- perfusion -- tissue preservation -- kidney -- liver -- kidney perfusion -- liver perfusion -- tissue culture -- Amino Acids -- Animal -- Culture Media -- Dogs -- English Abstract -- In Vitro -- Organ Culture -- Organ Preservation -- Perfusion -- Tissue Preservation -- Kidney -- Liver

Scopus
Держатели документа:
L.V. Kirensky Inst. Phys., Siberian Branch, USSR Acad. Sci., Krasnoyarsk, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Barashkov, V.A.; Gitelzon, I.I.; Nefedov, V.P.; Trubachev, I.N.

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^a314.27.21^2VINITI
К 17

Калачева, Г. С.
Состав липидов люминесцирующего бесклеточного экстракта из светящихся бактерий [Текст] : научное издание / Г. С. Калачева, Е. С. Высоцкий, В. В. Межевикин // Биохимия. - 1985. - Т. 50, N 11. - С. 1811-1816 . - ISSN 0320-9725

ГРНТИ

31.27.21

31.27.25

РУБ 314.27.21 + 314.27.25
Рубрики:
ЛИПИДЫ
   ХРОМАТОГРАФИЯ
   БАКТЕРИИ
   СВЕТЯЩИЕСЯ
   PHOTOBACTERIUM LEIOGNATHI
   PHOSPHOLIPIDS
   TRIGLYCERIDES
   FATTY ACIDS
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Высоцкий, Е.С.; Межевикин, В.В.

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Nucleotide sequence of part of Photobacterium leiognathi lux region / B. A. Illarionov [et al.] // Nucleic Acids Research. - 1988. - Vol. 16, Is. 20. - P9855, DOI 10.1093/nar/16.20.9855 . - ISSN 0305-1048
Кл.слова (ненормированные):
bacterial protein -- luciferase -- article -- bacterial gene -- genetics -- molecular genetics -- nucleotide sequence -- Photobacterium -- Bacterial Proteins -- Base Sequence -- Genes, Bacterial -- Luciferase -- Molecular Sequence Data -- Photobacterium

Scopus
Держатели документа:
Krasnoyarsk State University, Krasnoyarsk, Russian Federation
Institute of Biophysics, Krasnoyarsk, Russian Federation
Institute of Clinical and Experimental Medicine, Novosibirsk, Russian Federation
Novosibirsk Institute of Bioorganic Chemistry, 630090, Novosibirsk, Lavrentjev prospect 8, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Illarionov, B.A.; Protopopova, M.V.; Karginov, V.A.; Mertvetsov, N.P.; Gitelson, J.I.

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NUCLEOTIDE-SEQUENCE OF PART OF PHOTOBACTERIUM-LEIOGNATHI LUX REGION [Text] / B. A. ILLARIONOV [et al.] // Nucleic Acids Res. - 1988. - Vol. 16, Is. 20. - P. 9855-9855, DOI 10.1093/nar/16.20.9855. - Cited References: 1 . - ISSN 0305-1048

РУБ Biochemistry & Molecular Biology

WOS
Держатели документа:
NOVOSIBIRSK BIOORGAN CHEM INST,LAVRENTJEV PROSPECT 8,NOVOSIBIRSK 630090,USSR
KRASNOYARSK STATE UNIV,KRASNOYARSK,USSR
KRASNOYARSK BIOPHYS INST,KRASNOYARSK,USSR
NOVOSIBIRSK CLIN & EXPTL MED INST,NOVOSIBIRSK,USSR
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
ILLARIONOV, B.A.; PROTOPOPOVA, M.V.; KARGINOV, V.A.; MERTVETSOV, N.P.; GITELSON, J.I.

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Green flavoprotein from P. leiognathi: purification, characterization and identification as the product of the lux G(N) gene / A. A. Raibekas // Journal of bioluminescence and chemiluminescence. - 1991. - Vol. 6, Is. 3. - P. 169-176 . - ISSN 0884-3996
Кл.слова (ненормированные):
bacterial protein -- flavoprotein -- amino acid sequence -- article -- bacterial gene -- chemistry -- genetics -- isolation and purification -- luminescence -- molecular genetics -- molecular weight -- Photobacterium -- Amino Acid Sequence -- Bacterial Proteins -- Flavoproteins -- Genes, Bacterial -- Luminescence -- Molecular Sequence Data -- Molecular Weight -- Photobacterium -- Support, U.S. Gov't, P.H.S.
Аннотация: A green flavoprotein (GFP) was isolated and purified to homogeneity from Photobacterium leiognathi, strain 208. GFP is a homodimer of molecular weight 54,000 and contains two molecules of an unusual flavin per molecule of protein. Various biochemical characteristics including isoelectric point, trypsin and chymotrypsin degradation, SDS and temperature influence on subunit dissociation and the dissociation of the flavin chromophore, were investigated. The sequence of 23 N-terminal amino acids was determined and found to be concurrent with the N-terminal amino acid sequence encoded by the lux G(N) gene of P. leiognathi. This fact suggests that GFP is a structural component of the Photobacterium luminescence system.

Scopus
Держатели документа:
Institute of Biophysics, USSR Academy of Sciences, Krasnoyarsk. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Raibekas, A.A.

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FREE FATTY-ACIDS OF WATER-SURFACE FILM AS INDICATORS OF PHASES OF PHYTOPLANKTON SEASONAL DYNAMICS IN THE SYDINSKY BAY OF THE KRASNOYARSK RESERVOIR [Текст] / M. I. GLADYSHEV, G. S. KALACHOVA, N. N. SUSHCHIK // DOKLADY AKADEMII NAUK SSSR. - 1991. - Vol. 318, Is. 4. - С. 1017-1020. - Cited References: 9 . - ISSN 0002-3264

РУБ Multidisciplinary Sciences
Рубрики:
MICROLAYER
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
GLADYSHEV, M.I.; KALACHOVA, G.S.; SUSHCHIK, N.N.

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Growth and luminescence of luminous bacteria promoted by agents of microbial origin. / E. K. Rodicheva [et al.] // Journal of bioluminescence and chemiluminescence. - 1993. - Vol. 8, Is. 6. - P293-299 . - ISSN 0884-3996
Кл.слова (ненормированные):
amino acid -- carbohydrate -- folic acid -- luciferase -- nitrogen -- riboflavin -- article -- biosynthesis -- culture medium -- electron microscopy -- growth, development and aging -- kinetics -- luminescence -- metabolism -- Photobacterium -- physiology -- time -- ultrastructure -- Vibrio -- Amino Acids -- Carbohydrates -- Culture Media -- Folic Acid -- Kinetics -- Luciferase -- Luminescence -- Microscopy, Electron -- Nitrogen -- Photobacterium -- Riboflavin -- Time Factors -- Vibrio
Аннотация: The examination of four species of luminous bacteria Photobacterium leiognathi, Photobacterium phosphoreum, Vibrio fischeri and Vibrio harveyi has enabled us to reveal some nutrient medium components effecting growth, luminescence intensity and luciferase synthesis. These agents are nucleic components (nucleotides, nucleotides and amine bases), amino acids and vitamins, which are part of hydrolysates from the biomass of various lithotrophic microorganisms, hydrogen-oxidizing, iron-oxidizing and carboxydobacteria. The effect of promoting agents essentially alters the physiological state and ultrastructure of the cells of luminous bacteria and increases luciferase biosynthesis two- to three-fold compared to a control.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Krasnoyarsk. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Rodicheva, E.K.; Trubachev, I.N.; Medvedeva, S.E.; Egorova, O.I.; , U - Shitova LYu

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COMPOSITION OF FREE FATTY-ACIDS IN MEDIUM OF BLUE-GREEN-ALGAE SPIRULINA-PLATENSIS UNDER LIMITATION OF GROWTH [Текст] / M. I. GLADYSHEV, N. N. SUSHCHIK, G. S. KALACHEVA // Dokl. Akad. Nauk. - 1993. - Vol. 329, Is. 4. - С. 521-523. - Cited References: 6 . - ISSN 0869-5652

РУБ Multidisciplinary Sciences

: 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
GLADYSHEV, M.I.; SUSHCHIK, N.N.; KALACHEVA, G.S.

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10.

Free fatty acids of surface film of water in the Sydinsky Bay of the Krasnoyarsk Reservoir / M. I. Gladyshev, G. S. Kalachova, N. N. Sushchik // Internationale Revue der Gesamten Hydrobiologie. - 1993. - Vol. 78, Is. 4. - P575-587 . - ISSN 0020-9309

Scopus
Держатели документа:
Inst.Biophys., Akademgorodok,Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gladyshev, M.I.; Kalachova, G.S.; Sushchik, N.N.

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11.

Effect of carbon monoxide on metabolism and ultrastructure of carboxydobacteria / T. G. Volova [et al.] // World Journal of Microbiology and Biotechnology. - 1993. - Vol. 9, Is. 2. - P160-163, DOI 10.1007/BF00327827 . - ISSN 0959-3993
Кл.слова (ненормированные):
Carbon monoxide -- carboxydobacteria -- cytochromes -- hydrogenase -- Seliberia carboxydohydrogena
Аннотация: Growth of Seliberia carboxydohydrogena was inhibited by CO at 10 to 40% (v/v), resulting in increased substrate utilization and enhanced synthesis of cytochromes and cyclopropane and saturated fatty acids. The bacteria showed increased formation of new membrane structures, with pronounced folding of their cell walls. В© 1993 Rapid Communications of Oxford Ltd.

Scopus
Держатели документа:
Siberian Branch, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Guseinov, O.A.; Kalacheva, G.S.; Medvedeva, S.E.; Puzyr, A.A.

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12.

ACCUMULATION OF POLYHYDROXYBUTYRATE BY THE AEROBIC CO-OXIDIZING BACTERIUM SELIBERIA-CARBOXYDOHYDROGENA [Text] / T. G. VOLOVA, G. S. KALACHEVA, V. M. KONSTANTINOVA // Microbiology. - 1994. - Vol. 63, Is. 2. - P109-112. - Cited References: 20 . - 4. - ISSN 0026-2617

РУБ Microbiology

Аннотация: The synthesis of polyhydroxybutyrate by the carboxydobacterium Seliberia carboxydohydrogena under various growth conditions was studied. The bacterium was able to accumulate poly-beta-hydroxybutyric acid and a heteropolymer that was a product of copolymerization of hydroxybutyric and hydroxyvaleric acids. Nitrogen and sulfur exerted the strongest effect on polymer accumulation in carboxydobacteria: The maximum polymer concentrations in cells reached 28%. Specimens of polyhydroxybutyrate films were obtained, and their degradability by soil microflora was shown.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
VOLOVA, T.G.; KALACHEVA, G.S.; KONSTANTINOVA, V.M.

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13.

Rapid assay of fatty acid composition using a portable high-performance liquid chromatograph for monitoring aquatic ecosystems / N. N. Sushchik [et al.] // Journal of Chromatography A. - 1995. - Vol. 695, Is. 2. - P223-228, DOI 10.1016/0021-9673(94)01090-2 . - ISSN 0021-9673
Кл.слова (ненормированные):
fatty acid -- alga -- article -- culture medium -- ecology -- high performance liquid chromatography -- instrument -- methodology -- priority journal -- ultraviolet spectrophotometry -- water analysis
Аннотация: The chromatographic conditions presented allowed the separation of the nitrophenacyl derivatives of standards of eleven free fatty acids (FFA) using a portable high-performance chromatograph, suitable for use aboard a research vessel. A statistically significant linear correlation between UV absorbance and amount of the analytes injected was obtained. The method was tested on FFA from algae cultural media. The method can be used for the ecological monitoring of natural waters.

Scopus
Держатели документа:
Institute Biophysics, Akademgorodok, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sushchik, N.N.; Gladyshev, M.I.; Kalachova, G.S.; Guseynova, V.E.

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14.

Composition of free fatty acids of the surface film of water and kinetic of self-purification from phenol in forest pond at ''bloom'' of Volvox aureus [Текст] / M. I. Gladyshev [и др.] // Dokl. Akad. Nauk. - 1996. - Vol. 349, Is. 6. - С. 840-843. - Cited References: 5 . - ISSN 0869-5652

РУБ Multidisciplinary Sciences
Рубрики:
KRASNOYARSK RESERVOIR
MODELS
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gladyshev, M.I.; Sushchik, N.N.; Kalacheva, G.S.; Shchur, L.A.

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15.

Extra-cellular free fatty acids in batch culture of Spirulina platensis at increased and decreased temperature [Текст] / M. I. Gladyshev, N. N. Sushchik, G. S. Kalacheva // Dokl. Akad. Nauk. - 1996. - Vol. 347, Is. 6. - С. 834-836. - Cited References: 7 . - ISSN 0869-5652

РУБ Multidisciplinary Sciences

: 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
Gladyshev, M.I.; Sushchik, N.N.; Kalacheva, G.S.

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16.

Mineralization of wastes of human vital activity and plants to be used in a life support system [Text] / Y. A. Kudenko, I. V. Gribovskaya, R. A. Pavlenko // Acta Astronaut. - 1997. - Vol. 41, Is. 3. - P. 193-196, DOI 10.1016/S0094-5765(97)00215-4. - Cited References: 8 . - ISSN 0094-5765

РУБ Engineering, Aerospace

Аннотация: Available methods for mineralizing wastes of human activity and inedible biomass of plants used in this country and abroad are divided into two types: dry mineralization at high temperatures up to 1270 K with subsequent partial dissolution of the ash and the other-wet oxidation by acids. In this case mineralization is performed at a temperature of 470-460 K and a pressure of 220-270 atmospheres in pure oxygen with the output of mineral solution and dissoluble sediments in the form of scale. The drawback of the first method is the formation of dioxins, CO, SO2, NO2 and other toxic compounds. The latter method is too sophisticated and is presently confined to bench testing. The here proposed method to mineralize the wastes is in mid-position between the thermal and physical chemical methods. At a temperature of 89-90 degrees C the mixture was exposed to a controlled electromagnetic field at normal atmospheric pressure. The method merits simplicity, reliability, produces no dissoluble sediment or emissions noxious for human and plants. The basic difference from the above said methods is to employ as an oxidizer atomic oxygen, its active forms including OH-radicals with hydrogen peroxide as the sourer. Hydrogen peroxide can be produced with electric power from water inside the Life Support System (LSS). (C) 1998 Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kudenko, Y.A.; Gribovskaya, I.V.; Pavlenko, R.A.

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17.

Mineralization of wastes of human vital activity and plants in LSS [Text] / Y. A. Kudenko, I. V. Gribovskaya, R. A. Pavlenko ; ed. ya, IV Gribovs // SIXTH EUROPEAN SYMPOSIUM ON SPACE ENVIRONMENTAL CONTROL SYSTEMS, VOLS 1 AND 2. Ser. ESA SPECIAL PUBLICATIONS : EUROPEAN SPACE AGENCY, 1997. - Vol. 400: 6th European Symposium on Space Environmental Control Systems (MAY 20-22, 1997, NOORDWIJK, NETHERLANDS). - P. 803-806. - Cited References: 0 . - ISBN 0379-6566. - ISBN 92-9092-283-4

РУБ Engineering, Aerospace

Аннотация: Available methods for mineralizing wastes of human activity and inedible biomass of plants used in this country and abroad are divided into two types: dry mineralization at high temperatures up to 1270 degrees K with subsequent partial dissolution of the ash and the other wet oxidation by acids. In this case mineralization is performed at the temperature of 470-460 degrees K and the pressure of 220-270 atmospheres in pure oxygen with the output of mineral solution and dissoluble sediments in the form of scale. The drawback of the former method is formation of dioxins, CO, SO2, NO2 and other toxic compounds. The latter method is too sophisticated and presently is confined to bench testing. The proposed method to mineralize the wastes is in mid-position between the thermal and physical chemical methods. At the temperature of 80-90 degrees C the mixture was exposed to controlled electromagnetic field at normal atmospheric pressure. The method merits simplicity, reliability, produces no dissoluble sediment, emissions noxious for humans and plants. The basic difference from the above said methods is to employ for oxidizer atomic oxygen, its active forms including OH-radicals with hydrogen peroxide as the source. Hydrogen peroxide can be produced with electric power from water inside LSS.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kudenko, Y.A.; Gribovskaya, I.V.; Pavlenko, R.A.; Gribovs, ya, IV \ed.\

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18.

Determination of the bacterial concentration in freshwater reservoirs and streams by fluorometry [Text] / L. A. Shchur [et al.] // Microbiology. - 1997. - Vol. 66, Is. 6. - P. 726-728. - Cited References: 8 . - ISSN 0026-2617

РУБ Microbiology

Кл.слова (ненормированные):
concentration and biomass of bacterioplankton -- fluorescence -- regression equation
Аннотация: A simple fluorometric method is proposed for rapid determination of the concentration or biomass of bacterioplankton in various natural freshwater reservoirs and streams directly under field conditions. Fluorescamine, which specifically binds to proteins and amino acids in microbial cells, was used as the fluorescent marker. Regression equations were calculated on the basis of the results obtained. The coefficient of correlation between the fluorescence intensity and the bacterial biomass (cell number) was found to be 0.93 (0.89) for the total body of data obtained in the reservoirs studied.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shchur, L.A.; Aponasenko, A.D.; Lopatin, V.N.; Filimonov, V.S.; Shepelevich, N.V.

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19.

Specific free fatty acids of the surface film of water as probable markers of mortality of bacterioplankton [Текст] / M. I. Gladyshev [и др.] // Dokl. Akad. Nauk. - 1997. - Vol. 352, Is. 3. - С. 427-430. - Cited References: 7 . - ISSN 0869-5652

РУБ Multidisciplinary Sciences
Рубрики:
AQUATIC ECOSYSTEMS
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gladyshev, M.I.; Sushchik, N.N.; Kalacheva, G.S.; Shchur, L.A.

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20.

Studies of biodegradation of microbial polyhydroxyalkanoates / T. G. Volova [et al.] // Applied Biochemistry and Microbiology. - 1998. - Vol. 34, Is. 5. - P488-492 . - ISSN 0003-6838
Аннотация: Biodegradation of polyhydroxyalkanoates (PHA) - polymers of hydroxylated fatty acids - differing in chemical composition, crystallinity, melting temperature, and molecular weight was studied in soil and water environments under conditions of varying ambient temperature and pH. Under similar conditions, homogeneous films of polyhydroxybutyrate (PHB) with different initial properties were degraded at virtually equal rates. Hydroxybutyrate-hydroxyvalerate copolymers with a lower crystallinity and different microstructure in comparison to PHB were degraded at a 20 to 30% higher rate on average. The ambient temperature significantly influenced the rate of PHA biodegradation.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Belyaeva, O.G.; Plotnikov, V.F.; Puzyr', A.P.

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