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Nutritional biomarkers: Current view and future perspectives / S. Pande [et al.] // Crit. Rev. Food Sci. Nutr. - 2018. - Vol. 58, Is. 18. - P3055-3069, DOI 10.1080/10408398.2017.1350136. - Cited References:163. - The research was partially supported by the Russian Foundation for Basic Research [Grant No 16-06-00-439], the Russian Ministry of Education, Post-Doctoral Program of Project "5-100" [Grant No. M 2.2.3] and the Russian Foundation for Basic Research in collaboration with Krasnoyarsk Regional Fund for Scientific and Technical Activity Support [project No 16-44-242126]. . - ISSN 1040-8398. - ISSN 1549-7852

РУБ Food Science & Technology + Nutrition & Dietetics
Рубрики:
PROMOTES CELL-SURVIVAL
FATTY-ACID OXIDATION
CALORIE RESTRICTION
Кл.слова (ненормированные):
Sirtuins -- NAD -- biomarker -- proteomics -- calorie restriction -- longitivity
Аннотация: There is a poor relationship between nutrient intake and existing nutritional biomarkers due to variety of factors affecting their sensitivity and specificity. To explore the impact of nutrients at molecular level and devising a sensitive biomarker, proteomics is a central technology with sirtuins as one of the most promising nutritional biomarker. Sirtuins (seven mammalian sirtuins reported so far) have been reported to perform protein deacetylases and ADP-ribosyltransferases activity. It is distributed in different cellular compartments thereby controlling several metabolic processes. Sirtuins are oxidized nicotinamide adenine dinucleotide dependent, which implicates a direct effect of the metabolic state of the cell on its activity. Calorie restriction upregulates the mammalian sirtuin protein levels in variety of tissues and organs where it acts upon both histone and nonhistone substrates. Sirtuin senses nutrient availability and impacts gluconeogenesis, glycolysis, and insulin sensitivity. It deacetylates and inhibits the nuclear receptor that activates fat synthesis and adipogenesis in the body, leading to fat loss and bringing favorable cellular and health changes. Sirtuins mediates intracellular response that promotes cell survival, DNA damage repair thereby increasing the cell longitivity. The activation of sirtuins brings a wide spectrum of other health benefits and its activity levels are indicative of nutritional status as well as disease progression in cancer, inflammation, obesity, cardiovascular diseases, and viral infections. There are several foods that activate sirtuin activity and offer significant health benefits by their consumption.

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Держатели документа:
Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Dept Biophys, Lab Bioluminescent Biotechnol, Krasnoyarsk, Russia.
Krasnoyarsk State Med Univ, Krasnoyarsk, Russia.
RAS, Siberian Branch, Inst Biophys, Fed State Budgetary Sci Inst, Krasnoyarsk, Russia.
Fed State Budgetary Sci Inst, Sci Res Inst Med Problems North, Krasnoyarsk, Russia.

Доп.точки доступа:
Pande, Shubhra; Kratasyuk, Valentina A.; Medvedeva, Nadezhda N.; Kolenchukova, Oxana A.; Salmina, Alla B.; Kratazyuk, Valentina; KAYITESI, EUGENIE; Russian Foundation for Basic Research [16-06-00-439]; Russian Ministry of Education [M 2.2.3]; Russian Foundation for Basic Research; Krasnoyarsk Regional Fund for Scientific and Technical Activity Support [16-44-242126]

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Estimating levels of light emission and extracellular peroxidase activity of mycelium of luminous fungus Neonothopanus nambi treated with ?-glucosidase / O. A. Mogilnaya, N. O. Ronzhin, V. S. Bondar // Curr. Res. Environ. Appl. Mycol. J. Fungal. - 2018. - Vol. 8, Is. 1. - P75-85, DOI 10.5943/cream/8/1/6 . - ISSN 2229-2225
Кл.слова (ненормированные):
Basidiomycetes -- Cell wall -- Luminescence -- Polysaccharide sheath
Аннотация: The present study estimates the level of extracellular peroxidase activity and light emission intensity of mycelium of luminescent basidiomycete Neonothopanus nambi treated with ?-glucosidase. A hypothesis has been proposed that treatment with ?-glucosidase may trigger biochemical mechanisms of activation of ROS (primarily hydrogen peroxide) generation in N. nambi mycelium. The results obtained indicate that the enzyme causes partial disintegration of the slimy sheath of fungal hyphae and intracellular matrix, which leads to release of the extracellular peroxidases to the incubation medium. Mycelial cells treated with the enzyme reach the peak of their luminescence sooner. It has been assumed that partial loss of extracellular peroxidases, as important enzymes of antioxidant defense, may be compensated for by an increase in the level of light emission by the fungus. © 2018 Beijing Academy of Agriculture and Forestry Sciences.

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Держатели документа:
Institute of Biophysics, Siberian Branch of Russian Academy of Science, Federal Research Center Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Mogilnaya, O. A.; Ronzhin, N. O.; Bondar, V. S.

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Radioactive particles in the Yenisei River floodplain (Russia): Characterization, leaching and potential effects in the environment / A. Bolsunovsky, M. Melgunov // J. Environ. Radioact. - 2019. - Vol. 208-209, DOI 10.1016/j.jenvrad.2019.105991 . - ISSN 0265-931X
Кл.слова (ненормированные):
Fuel particles -- Incidents at plutonium reactors -- Leaching experiments -- Low doses -- Plant bioassays -- The Yenisei river floodplain -- Aquatic ecosystems -- Aquatic organisms -- Banks (bodies of water) -- Floods -- Fuel gages -- Fuels -- Gamma rays -- Leaching -- Plutonium -- Radiation effects -- Radioactivity -- Radioisotopes -- Flood plains -- Fuel particles -- Leaching experiments -- Low dose -- Plant bioassays -- Rivers -- Elodea canadensis
Аннотация: The operation of the Mining-and-Chemical Combine (MCC), the largest producer of weapons-grade plutonium in Russia, has resulted in radioactive contamination of the Yenisei River floodplain. Investigations carried out in Novosibirsk and Krasnoyarsk institutes have shown that the floodplain of the Yenisei downstream of the MCC is contaminated by radioactive particles (RP) of various types and activities. Analytical characterization of the RP showed that most of them were fuel particles, which were carried into the Yenisei after incidents at the MCC reactors. The plutonium and caesium isotope ratios (238Pu/239,240Pu; 137Cs/134Cs) vary substantially between the particles, indicating different source terms and time intervals when the RP were formed. In addition to fuel RP, there were particles that contained activation radionuclides. The experiment on dissolution of RP using the model solution (the simulated stomach fluid) showed different cumulative extractions of radionuclides from the particles: 60Co and 137Cs extractions were the lowest, the extracted fractions of europium and americium isotopes were the largest, and plutonium occupied an intermediate position. High concentrations of radionuclides in RP are sources of exposure of organisms in aquatic and terrestrial ecosystems to low radiation doses. The plant bioassays of the effects of ?-radiation from RP showed the effect of low doses of ?-radiation on growth parameters of aquatic plant Elodea canadensis growing in the Yenisei River floodplain. The presence of RP from different sources in the Yenisei River floodplain makes this region a unique site for studying environmental effects of the particles. © 2019 Elsevier Ltd

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Держатели документа:
Institute of Biophysics Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Institute of Geology and Mineralogy Siberian Branch of Russian Academy of Sciences, Novosibirsk, 630090, Russian Federation

Доп.точки доступа:
Bolsunovsky, A.; Melgunov, M.

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NAD(P)H:FMN-Oxidoreductase Functioning Under Macromolecular Crowding: In Vitro Modeling / A. E. Govorun, E. N. Esimbekova, V. A. Kratasyuk // Doklad. Biochem. Biophys. - 2019. - Vol. 486, Is. 1. - P213-215, DOI 10.1134/S160767291903013X . - ISSN 1607-6729
Аннотация: The functioning of NAD(P)H:FMN‑oxidoreductase (Red) from Vibrio fischeri under conditions of macromolecular crowding (MMC) simulated in vitro by adding biopolymers (starch and gelatin) was studied. The dissociation rate constants and the activation energies of dissociation of Red to the subunits were calculated, and the process of denaturation of Red was analyzed. It is shown that the functioning of Red both under conditions of MMC and in diluted solutions is the same. This result refutes the common belief that the native conformation of enzymes in vivo is stabilized due to MMC as compared to the in vitro conditions. © 2019, Pleiades Publishing, Ltd.

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Держатели документа:
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Govorun, A. E.; Esimbekova, E. N.; Kratasyuk, V. A.

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The fluorescence method for determining of photosynthetic apparatus reactivity in plant leaves / T. V. Nesterenko, V. N. Shikhov, A. A. Tikhomirov // Zhurnal Obshchei Biol. - 2019. - Vol. 80, Is. 3. - С. 187-199, DOI 10.1134/S0044459619030060. - Cited References:31 . - ISSN 0044-4596

РУБ Biology
Рубрики:
ELECTRIC-POTENTIAL DIFFERENCE
FREQUENTLY ASKED QUESTIONS
CHLOROPHYLL
Аннотация: Presently, the most promising way of studying, forecasting, and enhancing of organisms' tolerance to harsh environmental impacts is considered to be the estimation of initial functional state of an organism's regulatory systems. To resolve the problem of photosynthetic apparatus (PSA) tolerance to harsh impacts at the level of such a complicated functional system as a plant leaf, it is necessary to assess integral responses of the leaf's PSA to the impact. At that, simple and versatile traits may have certain advantages. At present, chlorophyll fluorescence seems to be one of the main indices of PSA activity, which can be measured relatively fast and easy. One of the possible approaches to operational integrative assessment of PSA activity may consist in usage of the parameters introduced for the curves of chlorophyll fluorescence induction (CFI) slow phase. Temporal patterns of CFI are of special interest. The simplest index T-0.5 (i.e., half-time of chlorophyll fluorescence intensity decrease during the slow phase of CFI) appears to be an integral characteristic of activation rate with regard to a number of photo-assimilation and photo-protective processes in leaves. On basis of the studies, conducted earlier, and published data, we have analyzed the behavior of T-0.5 parameter with comparison to other CFI traits (namely: qN - non-photochemical quenching coefficient, ETR - electron transport rate, Phi(PSII) - effective quantum yield of PSII photochemistry, F-p/F-T ratio) under different conditions. The influence of leaf senescence, changes in intensity of excitation light, slight dehydration of plants and their recovery from water deficiency have been examined. The pattern of T-0.5 behavior, observed in laboratory experiments, and the results of its comparison with other indices of CFI give occasion to propose the usage of T-0.5 for indirect estimation of PSA activity when operational integrative monitoring of PSA state is required. Further studies are necessary for establishing quantitative relationships between PSA activity and fluorescence parameter T-0.5 under specific stress conditions.

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Держатели документа:
RAS, Siberian Branch, Inst Biophys, Acad Gorodok 50-50, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Nesterenko, T. V.; Shikhov, V. N.; Tikhomirov, A. A.

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Bioluminescent and biochemical properties of Cys-free Ca2+-regulated photoproteins obelin and aequorin / E. V. Eremeeva, E. S. Vysotski // J. Photochem. Photobiol. B-Biol. - 2017. - Vol. 174. - P97-105, DOI 10.1016/j.jphotobio1.2017.07.021. - Cited References:54. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences (projects 03562016-0712 and 0356-2015-0103) and the RFBR grant 17-04-00764. . - ISSN 1011-1344

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
SEQUENCE-ANALYSIS
   APO-OBELIN
   INTRINSIC FLUORESCENCE
   COELENTERAZINE
Кл.слова (ненормированные):
Bioluminescence -- Coelenterazine -- Photoprotein -- Coelenteramide -- Cysteine -- Serine
Аннотация: Bioluminescence of a variety of marine coelenterates is determined by Ca2+-regulated photoproteins. A strong interest in these proteins is for their wide analytical potential as intracellular calcium indicators and labels for in vitro binding assays. The presently known hydromedusan Ca2+-regulated photoproteins contain three (aequorin and clytin) or five (obelin and mitrocomin) cysteine residues with one of them strictly conserved. We have constructed Cys-free aequorin and obelin by substitution of all cysteines to serine residues. Such mutants should be of interest for researchers by the possibility to avoid the incubation with dithiothreitol (or p-mercaptoethanol) required for producing an active photoprotein that is important for some prospective analytical assays in which the photoprotein is genetically fused with a target protein sensitive to the reducing agents. Cys-free mutants were expressed in Escherichia coil, purified, and characterized regarding the efficiency of photoprotein complex formation, functional activity, and conformational stability. The replacement of cysteine residues has been demonstrated to affect different properties of aequorin and obelin. Cys-free aequorin displays a two-fold lower specific bioluminescence activity but preserves similar activation properties and light emission kinetics compared to the wild -type aequorin. In contrast, Cys-free obelin retains only 10% of the bioluminescence activity of wild-type obelin as well as binding coelenterazine and forming active photoprotein much less effectively. In addition, the substitution of Cys residues drastically changes the bioluminescence kinetics of obelin completely eliminating a "fast" component from the light signal decay curve. At the same time, the replacement of Cys residues increases conformational flexibility of both aequorin and obelin molecules, but again, the effect is more prominent in the case of obelin. The values of thermal midpoints of unfolding (Tm) were determined to be 53.3 0.2 and 44.6 0.4 C for aequorin and Cys-free aequorin, and 49.1 0.1 and 28.8 0.3 C for obelin and Cys-free obelin, respectively. Thus, so far only Cys-free aequorin is suitable as a partner for fusing with a tag sensitive to reducing agents since the aequorin mutant preserves almost 50% of the bioluminescent activity and can be produced with a substantial yield.

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RAS, Photobiol Lab, Inst Biophys, Fed Res Ctr,Krasnoyarsk Sci Ctr,SB, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Eremeeva, Elena V.; Vysotski, Eugene S.; Russian Academy of Sciences [03562016-0712, 0356-2015-0103]; RFBR [17-04-00764]

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Exposure of luminous marine bacteria to low-dose gamma-radiation / N. S. Kudryasheva [et al.] // J. Environ. Radioact. - 2017. - Vol. 169-170. - P64-69, DOI 10.1016/j.jenvrad.2017.01.002 . - ISSN 0265-931X
Кл.слова (ненормированные):
Bioassay -- Low-dose gamma-radiation -- Luminous marine bacteria -- Mutagenic effect -- Radiotoxicity -- Temperature dependence -- Bacteria -- Bioassay -- Bioluminescence -- Gamma rays -- Ionizing radiation -- Irradiation -- Phosphorescence -- Physiological models -- Radiation effects -- Temperature distribution -- Low dose -- Marine bacterium -- Mutagenic effect -- Radiotoxicity -- Temperature dependence -- Radiation -- Bacteria (microorganisms) -- Photobacterium phosphoreum
Аннотация: The study addresses biological effects of low-dose gamma-radiation. Radioactive 137Cs-containing particles were used as model sources of gamma-radiation. Luminous marine bacterium Photobacterium phosphoreum was used as a bioassay with the bioluminescent intensity as the physiological parameter tested. To investigate the sensitivity of the bacteria to the low-dose gamma-radiation exposure (?250 mGy), the irradiation conditions were varied as follows: bioluminescence intensity was measured at 5, 10, and 20°С for 175, 100, and 47 h, respectively, at different dose rates (up to 4100 ?Gy/h). There was no noticeable effect of gamma-radiation at 5 and 10°С, while the 20°С exposure revealed authentic bioluminescence inhibition. The 20°С results of gamma-radiation exposure were compared to those for low-dose alpha- and beta-radiation exposures studied previously under comparable experimental conditions. In contrast to ionizing radiation of alpha and beta types, gamma-emission did not initiate bacterial bioluminescence activation (adaptive response). As with alpha- and beta-radiation, gamma-emission did not demonstrate monotonic dose-effect dependencies; the bioluminescence inhibition efficiency was found to be related to the exposure time, while no dose rate dependence was found. The sequence analysis of 16S ribosomal RNA gene did not reveal a mutagenic effect of low-dose gamma radiation. The exposure time that caused 50% bioluminescence inhibition was suggested as a test parameter for radiotoxicity evaluation under conditions of chronic low-dose gamma irradiation. © 2017 Elsevier Ltd

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Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, 50/50 Akademgorodok, Krasnoyarsk, Russian Federation
Siberian Federal University, 79 Svobodny Prospect, Krasnoyarsk, Russian Federation
Krasnoyarsk State Agrarian University, 90 Mira Prospect, Krasnoyarsk, Russian Federation
SB RAS Genomics Core Facility, Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russian Federation

Доп.точки доступа:
Kudryasheva, N. S.; Petrova, A. S.; Dementyev, D. V.; Bondar, A. A.

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577.34
И 73


    Интенсивность накопления урана-238 представителями разных экологических уровней экосистемы р. Енисей [Текст] / Сибирское Отделение Российской академии наук, Институт биофизики СО РАН ; Институт биофизики СО РАН // Вестник Томского государственного университета. Биология. - 2016. - № 2 (34) . - С. 161-171DOI 10.17223/19988591/34/11
   Перевод заглавия: Accumulation of uranium-238 by representatives of different ecological levels in the Yenisei River ecosystem

УДК

577.34

597.5

Кл.слова (ненормированные):
коэффициент накопления -- трофический уровень -- Philolimnogammarus viridis -- Fointinalis antipyretica -- Thymallus arcticus -- Esox lucius)
Аннотация: В результате многолетних исследований (2010-2013) получены данные по накоплению урана-238 в гидробионтах разных трофических уровней экосистемы р. Енисей. Пробы водного мха (Fointinalis antipyretica), зообентоса (Philolimnogammarus viridis и Ph. Cyaneus), сибирского хариуса (Thymallus arcticus) и щуки (Esox lucius) отбирали в районе ранее зарегистрированного повышенного содержания урана в воде р. Енисей (вблизи радиоактивных сбросов Горно-химического комбината Росатома). Показано, что уран способен эффективно накапливаться из воды в биомассе всех исследованных гидробионтов. Максимальные коэффициенты накопления (КН) урана получены для водного мха, что многократно превышает КН урана для зообентоса и мышечной ткани рыб. Средние значения КН урана для зообентоса статистически значимо превышают КН в мышцах рыб за весь период наблюдения. Не выявлено статистически значимых отличий в накоплении урана рыбами разного трофического уровня (хариус и щука)
The aim of the research was a comparative evaluation of the intensity of uranium-238 accumulation in the biomass of aquatic organisms, occupying different trophic levels in the ecosystem of the Yenisei river. Previously, uranium-238 at concentrations higher than the background ones was detected in water and sediment samples of the Yenisei River collected close to the discharge site of one of the Rosatom facilities (the Mining-and-Chemical Combine). However, radioecological studies of the Yenisei River ecosystem have not focused on uranium isotopes in aquatic organisms so far. The toxic effects of uranium on living organisms are known to be based on its both radioactive and chemical properties. We conducted a study on accumulation of uranium-238 in aquatic organisms at different trophic levels in the Yenisei River ecosystem between 2010 and 2013. We collected samples of aquatic moss (Fointinalis antipyretica), zoobenthos (Philolimnogammarus viridis and Ph. cyaneus), arctic grayling (Thymallus arcticus), and pike (Esox lucius) from the Yenisei region at a distance of 85-90 km downstream of Krasnoyarsk (5-10 km downstream of the radioactive discharge of the Mining-and-Chemical Combine). Data on samples are shown in Table 1. We determined uranium concentration in the samples of aquatic organisms by neutron activation analysis, in water - by ICP-MS (Table 2). The study shows that uranium can be effectively concentrated from water and accumulate in the biomass of all aquatic organisms used in experiments. The highest uranium concentration factors (CFs) were obtained for aquatic moss, and they were several times higher than the uranium CFs for zoobenthos and fish muscles. Based on the uranium concentration from water, all aquatic organisms of the Yenisei River used in this study can be ranked as follows: aquatic moss > gammarids > grayling = pike. No statistically significant difference has been found between uranium accumulation by fish of different trophic levels (Arctic grayling and pike). The uranium CFs obtained in this study is comparable with the literature data on the CFs of artificial radionuclides for aquatic moss and fish of the Yenisei River

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Держатели документа:
Институт биофизики Сибирского отделения Российской академии наук : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Болсуновский, Александр Яковлевич; Трофимова, Елена Александровна; Дементьев, Дмитрий Владимирович; Карпов, Антон Дмитриевич; Институт биофизики Сибирского отделения Российской Академии наук (Красноярск)

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Is bacterial luminescence response to low-dose radiation associated with mutagenicity? / T. V. Rozhko [et al.] // J. Environ. Radioact. - 2017. - Vol. 177. - P261-265, DOI 10.1016/j.jenvrad.2017.07.010 . - ISSN 0265-931X
Кл.слова (ненормированные):
Bioassay -- DNA -- Low-dose radiation -- Luminous marine bacteria -- Mutations -- Bacteria -- Bioassay -- Bioluminescence -- Chemical activation -- DNA -- DNA sequences -- Genes -- Ionizing radiation -- Kinetics -- Luminescence -- Nucleic acids -- Phosphorescence -- Physiological models -- Radioisotopes -- Bacterial suspensions -- Beta-emitting radionuclides -- Low dose radiation -- Luminescence intensity -- Marine bacterium -- Mutations -- Photobacterium phosphoreum -- Physiological parameters -- Radiation -- Bacteria (microorganisms) -- Photobacterium phosphoreum
Аннотация: Luminous marine bacteria are widely used in bioassays with luminescence intensity being a physiological parameter tested. The purpose of the study was to determine whether bacterial genetic alteration is responsible for bioluminescence kinetics change under low-dose radiation exposure. The alpha-emitting radionuclide 241Am and beta-emitting radionuclide 3H were used as the sources of low-dose ionizing radiation. Changes of bioluminescence kinetics of Photobacterium phosphoreum in solutions of 241Am(NO3)3, 7 kBq/L, and tritiated water, 100 MBq/L, were studied; bioluminescence kinetics stages (absence of effect, activation, and inhibition) were determined. Bacterial suspension was sampled at different stages of the bioluminescent kinetics; the doses accumulated by the samples were close or a little higher than a tentative limit of a low-dose interval: 0.10 and 0.85 Gy for 241Am, or 0.11 and 0.18 Gy for 3H. Sequence analysis of the 16S ribosomal RNA gene did not reveal a mutagenic effect of low-dose alpha and beta radiation in the bacterial samples. Previous results on bacterial DNA exposed to low-dose gamma radiation (0.25 Gy) were analyzed and compared to those for alpha and beta irradiation. It is concluded that bioluminescence activation and/or inhibition under the applied conditions of low-dose alpha, beta and gamma radioactive exposure is not associated with DNA mutations in the gene sequences tested. © 2017 Elsevier Ltd

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Krasnoyarsk State Medical Academy, 1 P.Zheleznyaka, Krasnoyarsk, Russian Federation
Siberian Federal University, 79 Svobodny Prospect, Krasnoyarsk, Russian Federation
SB RAS Genomics Core Facility, Institute of Chemical Biology and Fundamental Medicine SB RAS, 8 Lavrentiev Avenue, Novosibirsk, Russian Federation
Siberian State Technological University, LB, 29 Pobedy, Lesosibirsk, Krasnoyarsk Region, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, 50/50 Akademgorodok, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Rozhko, T. V.; Guseynov, O. A.; Guseynova, V. E.; Bondar, A. A.; Devyatlovskaya, A. N.; Kudryasheva, N. S.

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10.

Unique diversity of radioactive particles found in the Yenisei River floodplain / A. Bolsunovsky [et al.] // Sci Rep. - 2017. - Vol. 7. - Ст. 11132, DOI 10.1038/s41598-017-11557-7. - Cited References:22. - The authors are grateful to researchers of the Institute of Biophysics FRC KSC SB RAS (Krasnoyarsk, Russia) and the Institute of Geology and Mineralogy SB RAS (Novosibirsk, Russia) for their assistance in isolation and analysis of radioactive particles. Part of the work is also supported by the Research Council of Norway through its Centres of Excellence funding scheme, project number 223268/F50. Investigations of the particles were partially supported by IAEA (Project 17941). . - ISSN 2045-2322

РУБ Multidisciplinary Sciences
Рубрики:
CONTAINING CONTROL RODS
   TRANSURANIC ELEMENTS
   BOTTOM SEDIMENTS
   HOT
Аннотация: The long-term operation of three reactors and the radiochemical plant of the Mining-and-Chemical Combine (MCC), Russia's largest producer of weapons-grade plutonium, has resulted in radioactive contamination of the Yenisei River floodplain. From 1995 to 2016, we found more than 200 radioactive particles (RP) in the Yenisei floodplain, downstream of the MCC. Analytical characterization showed that most of the RP were fuel particles, which were carried into the river after incidents at the MCC reactors. Having compared the Cs-137/Cs-134 ratios in the particles, we determined three time intervals when the RP were formed. The plutonium isotope ratios (Pu-238/Pu-239,Pu-240) vary substantially between the particles and indicate several different source terms. In addition to fuel RP, we found particles that only contained activation products (Co-60 or europium isotopes). SEM and gamma-spectrometry showed that the cobalt particles could have originated from the corrosion of the reactor coolant system and the europium particles -from the damaged compensating rods. No europium particles have been found anywhere else in the world. The presence of RP from different sources (fuel, cobalt, and europium particles) in the Yenisei River floodplain makes this region a unique site for studying environmental effects of the particles. These RP represent point sources of radioecological significance.

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FRC Krasnoyarsk Sci Ctr SB RAS, Inst Biophys SB RAS, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Inst Geol & Mineral SB, Novosibirsk 630090, Russia.
Norwegian Univ Life Sci, Ctr Environm Radioact, As, Norway.

Доп.точки доступа:
Bolsunovsky, Alexander; Melgunov, Mikhail; Chuguevskii, Alexey; Lind, Ole Christian; Salbu, Brit; Research Council of Norway through its Centres of Excellence funding scheme [223268/F50]; IAEA [17941]

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11.

Bioluminescent and biochemical properties of Cys-free Ca2+-regulated photoproteins obelin and aequorin / E. V. Eremeeva, E. S. Vysotski // J. Photochem. Photobiol. B Biol. - 2017. - Vol. 174. - P97-105, DOI 10.1016/j.jphotobiol.2017.07.021 . - ISSN 1011-1344
Кл.слова (ненормированные):
Bioluminescence -- Coelenteramide -- Coelenterazine -- Cysteine -- Photoprotein -- Serine
Аннотация: Bioluminescence of a variety of marine coelenterates is determined by Ca2+-regulated photoproteins. A strong interest in these proteins is for their wide analytical potential as intracellular calcium indicators and labels for in vitro binding assays. The presently known hydromedusan Ca2+-regulated photoproteins contain three (aequorin and clytin) or five (obelin and mitrocomin) cysteine residues with one of them strictly conserved. We have constructed Cys-free aequorin and obelin by substitution of all cysteines to serine residues. Such mutants should be of interest for researchers by the possibility to avoid the incubation with dithiothreitol (or ?-mercaptoethanol) required for producing an active photoprotein that is important for some prospective analytical assays in which the photoprotein is genetically fused with a target protein sensitive to the reducing agents. Cys-free mutants were expressed in Escherichia coli, purified, and characterized regarding the efficiency of photoprotein complex formation, functional activity, and conformational stability. The replacement of cysteine residues has been demonstrated to affect different properties of aequorin and obelin. Cys-free aequorin displays a two-fold lower specific bioluminescence activity but preserves similar activation properties and light emission kinetics compared to the wild-type aequorin. In contrast, Cys-free obelin retains only ~ 10% of the bioluminescence activity of wild-type obelin as well as binding coelenterazine and forming active photoprotein much less effectively. In addition, the substitution of Cys residues drastically changes the bioluminescence kinetics of obelin completely eliminating a “fast” component from the light signal decay curve. At the same time, the replacement of Cys residues increases conformational flexibility of both aequorin and obelin molecules, but again, the effect is more prominent in the case of obelin. The values of thermal midpoints of unfolding (Tm) were determined to be 53.3 ± 0.2 and 44.6 ± 0.4 °C for aequorin and Cys-free aequorin, and 49.1 ± 0.1 and 28.8 ± 0.3 °C for obelin and Cys-free obelin, respectively. Thus, so far only Cys-free aequorin is suitable as a partner for fusing with a tag sensitive to reducing agents since the aequorin mutant preserves almost 50% of the bioluminescent activity and can be produced with a substantial yield. © 2017 Elsevier B.V.

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Держатели документа:
Photobiology Laboratory, Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Eremeeva, E. V.; Vysotski, E. S.

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12.

Toxicity and Antioxidant Activity of Fullerenol C-60,C-70 with Low Number of Oxygen Substituents / E. S. Kovel, A. G. Kicheeva, N. G. Vnukova [et al.] // Int. J. Mol. Sci. - 2021. - Vol. 22, Is. 12. - Ст. 6382, DOI 10.3390/ijms22126382. - Cited References:93. - This research was funded by RFBR, N18-29-19003; RFBR, Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science, N20-44-243001; and partly supported by the Program of the Federal Service for Surveillance on Consumer Rights Protection and HumanWellbeing, Fundamental Study 2020-2025 (Russian Federation). . - ISSN 1422-0067

РУБ Biochemistry & Molecular Biology + Chemistry, Multidisciplinary
Рубрики:
HUMIC SUBSTANCES
DETOXIFICATION PROCESSES
BIOLOGICAL-ACTIVITY
Кл.слова (ненормированные):
fullerenol -- toxicity -- antioxidant activity -- reactive oxygen species -- bioluminescent assay -- hormesis
Аннотация: Fullerene is a nanosized carbon structure with potential drug delivery applications. We studied the bioeffects of a water-soluble fullerene derivative, fullerenol, with 10-12 oxygen groups (F10-12); its structure was characterized by IR and XPS spectroscopy. A bioluminescent enzyme system was used to study toxic and antioxidant effects of F10-12 at the enzymatic level. Antioxidant characteristics of F10-12 were revealed in model solutions of organic and inorganic oxidizers. Low-concentration activation of bioluminescence was validated statistically in oxidizer solutions. Toxic and antioxidant characteristics of F10-12 were compared to those of homologous fullerenols with a higher number of oxygen groups:F24-28 and F40-42. No simple dependency was found between the toxic/antioxidant characteristics and the number of oxygen groups on the fullerene's carbon cage. Lower toxicity and higher antioxidant activity of F24-28 were identified and presumptively attributed to its higher solubility. An active role of reactive oxygen species (ROS) in the bioeffects of F10-12 was demonstrated. Correlations between toxic/antioxidant characteristics of F10-12 and ROS content were evaluated. Toxic and antioxidant effects were related to the decrease in ROS content in the enzyme solutions. Our results reveal a complexity of ROS effects in the enzymatic assay system.

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Держатели документа:
FRC KSC SB RAS, Inst Biophys SB RAS, Krasnoyarsk 660036, Russia.
FRC KSC SB RAS, Inst Phys SB RAS, Krasnoyarsk 660036, Russia.
FRC KSC SB RAS, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Kovel, Ekaterina S.; Kicheeva, Arina G.; Vnukova, Natalia G.; Churilov, Grigory N.; Stepin, Evsei A.; Kudryasheva, Nadezhda S.; Kovel, Ekaterina; RFBRRussian Foundation for Basic Research (RFBR) [N18-29-19003]; RFBR, Krasnoyarsk Territory; Krasnoyarsk Regional Fund of Science [N20-44-243001]; Program of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Fundamental Study 2020-2025 (Russian Federation)

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13.

Toxicity and antioxidant activity of fullerenol c60,70 with low number of oxygen substituents / E. S. Kovel, A. G. Kicheeva, N. G. Vnukova [et al.] // Int. J. Mol. Sci. - 2021. - Vol. 22, Is. 12. - Ст. 6382, DOI 10.3390/ijms22126382 . - ISSN 1661-6596
Кл.слова (ненормированные):
Antioxidant activity -- Bioluminescent assay -- Fullerenol -- Hormesis -- Reactive oxygen species -- Toxicity
Аннотация: Fullerene is a nanosized carbon structure with potential drug delivery applications. We studied the bioeffects of a water-soluble fullerene derivative, fullerenol, with 10-12 oxygen groups (F10-12); its structure was characterized by IR and XPS spectroscopy. A bioluminescent enzyme system was used to study toxic and antioxidant effects of F10-12 at the enzymatic level. Antioxidant characteristics of F10-12 were revealed in model solutions of organic and inorganic oxidizers. Low-concentration activation of bioluminescence was validated statistically in oxidizer solutions. Toxic and antioxidant characteristics of F10-12 were compared to those of homologous fullerenols with a higher number of oxygen groups:F24-28 and F40-42. No simple dependency was found between the toxic/antioxidant characteristics and the number of oxygen groups on the fullerene’s carbon cage. Lower toxicity and higher antioxidant activity of F24-28 were identified and presumptively attributed to its higher solubility. An active role of reactive oxygen species (ROS) in the bioeffects of F10-12 was demonstrated. Correlations between toxic/antioxidant characteristics of F10-12 and ROS content were evaluated. Toxic and antioxidant effects were related to the decrease in ROS content in the enzyme solutions. Our results reveal a complexity of ROS effects in the enzymatic assay system. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, FRC KSC SB RAS, Krasnoyarsk, 660036, Russian Federation
Institute of Physics SB RAS, FRC KSC SB RAS, Krasnoyarsk, 660036, Russian Federation
FRC KSC SB RAS, Krasnoyarsk, 660036, Russian Federation
Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, 660041, Russian Federation

Доп.точки доступа:
Kovel, E. S.; Kicheeva, A. G.; Vnukova, N. G.; Churilov, G. N.; Stepin, E. A.; Kudryasheva, N. S.

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14.

H2O-Bridged Proton-Transfer Channel in Emitter Species Formation in Obelin Bioluminescence / S. -F. Chen, E. S. Vysotski, Y. -J. Liu // J Phys Chem B. - 2021, DOI 10.1021/acs.jpcb.1c03985 . - Article in press. - ISSN 1520-6106
Кл.слова (ненормированные):
Amino acids -- Excited states -- Hydrogen bonds -- Molecular dynamics -- Molecular modeling -- Molecules -- Phosphorescence -- Proton transfer -- Quantum theory -- Fast protons -- Marine organisms -- Photoproteins -- Primary products -- Proton transfer process -- Quantum mechanics/molecular mechanics -- Reaction substrates -- Singlet excited state -- Theoretical calculations -- Transfer channel -- Bioluminescence
Аннотация: Bioluminescence of a number of marine organisms is conditioned by Ca2+-regulated photoprotein (CaRP) with coelenterazine as the reaction substrate. The reaction product, coelenteramide, at the first singlet excited state (S1) is the emitter of CaRP. The S1-state coelenteramide is produced via the decomposition of coelenterazine dioxetanone. Experiments suggested that the neutral S1-coelenteramide is the primary emitter species. This supposition contradicts with theoretical calculations showing that the anionic S1-coelenteramide is a primary product of the decomposition of coelenterazine dioxetanone. In this study, applying molecular dynamic (MD) simulations and the hybrid quantum mechanics/molecular mechanics (QM/MM) method, we investigated a proton-transfer (PT) process taking place in CaRP obelin from Obelia longissima for emitter formation. Our calculations demonstrate a concerted PT process with a water molecule as a bridge between anionic S1-coelenteramide and the nearest histidine residue. The low activation barrier as well as the strong hydrogen-bond network between the proton donor and the proton acceptor suggests a fast PT process comparable with that of the lifetime of excited anionic S1-coelenteramide. The existence of the PT process eliminates the discrepancy between experimental and theoretical studies. The fast PT process at emitter formation can also take place in other CaRPs. © 2021 American Chemical Society.

Scopus
Держатели документа:
School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai, 201418, China
Photobiology Laboratory, Institute of Biophysics SB RAS, Federal Research Center, Krasnoyarsk Science Center SB RAS, Krasnoyarsk, 660036, Russian Federation
Center for Advanced Materials Research, Advanced Institute of Natural Sciences, Beijing Normal University at Zhuhai, Zhuhai, 519087, China
Key Laboratory of Theoretical and Computational Photochemistry, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing, 100875, China

Доп.точки доступа:
Chen, S. -F.; Vysotski, E. S.; Liu, Y. -J.

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15.

Development of Cellular and Enzymatic Bioluminescent Assay Systems to Study Low-Dose Effects of Thorium / O. V. Kolesnik, T. V. Rozhko, M. A. Lapina [et al.] // Bioengineering-Basel. - 2021. - Vol. 8, Is. 12. - Ст. 194, DOI 10.3390/bioengineering8120194. - Cited References:77 . - ISSN 2306-5354

РУБ Biotechnology & Applied Microbiology + Engineering, Biomedical

Кл.слова (ненормированные):
bioassay -- bioluminescence -- luminous bacteria -- enzymes -- reactive oxygen -- species -- thorium -- low-dose exposure -- radiation hormesis
Аннотация: Thorium is one of the most widespread radioactive elements in natural ecosystems, along with uranium, it is the most important source of nuclear energy. However, the effects of thorium on living organisms have not been thoroughly studied. Marine luminescent bacteria and their enzymes are optimal bioassays for studying low-dose thorium exposures. Luminescent bioassays provide a quantitative measure of toxicity and are characterized by high rates, sensitivity, and simplicity. It is known that the metabolic activity of bacteria is associated with the production of reactive oxygen species (ROS). We studied the effects of thorium-232 (10(-11)-10(-3) M) on Photobacterium phosphoreum and bacterial enzymatic reactions; kinetics of bacterial bioluminescence and ROS content were investigated in both systems. Bioluminescence activation was revealed under low-dose exposures (<0.1 Gy) and discussed in terms of "radiation hormesis". The activation was accompanied by an intensification of the oxidation of a low-molecular reducer, NADH, during the enzymatic processes. Negative correlations were found between the intensity of bioluminescence and the content of ROS in bacteria and enzyme systems; an active role of ROS in the low-dose activation by thorium was discussed. The results contribute to radioecological potential of bioluminescence techniques adapted to study low-intensity radioactive exposures.

WOS
Держатели документа:
RAS, Krasnoyarsk Sci Ctr SB, Inst Biophys SB, Fed Res Ctr, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Biophys Dept, Krasnoyarsk 660041, Russia.
Krasnoyarsk State Med Acad, Krasnoyarsk 660022, Russia.
Natl Res Tomsk Polytech Univ, Tomsk 634050, Russia.

Доп.точки доступа:
Kolesnik, Olga V.; Rozhko, Tatiana V.; Lapina, Maria A.; Solovyev, Vladislav S.; Sachkova, Anna S.; Kudryasheva, Nadezhda S.; Kolesnik, Olga

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16.

H2O-Bridged Proton-Transfer Channel in Emitter Species Formation in Obelin Bioluminescence / S. F. Chen, E. S. Vysotski, Y. J. Liu // J. Phys. Chem. B. - 2021. - Vol. 125, Is. 37. - P10452-10458, DOI 10.1021/acs.jpcb.1c03985. - Cited References:50. - This work was supported by the Program of Shanghai Institute of Technology (no. YJ2016-42), the National Natural Science Foundation of China (21973005 and 21911530094), and the Russian Foundation for Basic Research (20-04-00085 and 19-14-53004). . - ISSN 1520-6106. - ISSN 1520-5207

РУБ Chemistry, Physical
Рубрики:
CHEMILUMINESCENT DECOMPOSITION
   FLUORESCENCE-SPECTRA
   MECHANISM
   QM/MM
Аннотация: Bioluminescence of a number of marine organisms is conditioned by Ca2+-regulated photoprotein (CaRP) with coelenterazine as the reaction substrate. The reaction product, coelenteramide, at the first singlet excited state (S-1) is the emitter of CaRP. The S-1-state coelenteramide is produced via the decomposition of coelenterazine dioxetanone. Experiments suggested that the neutral S-1-coelenteramide is the primary emitter species. This supposition contradicts with theoretical calculations showing that the anionic S-1-coelenteramide is a primary product of the decomposition of coelenterazine dioxetanone. In this study, applying molecular dynamic (MD) simulations and the hybrid quantum mechanics/molecular mechanics (QM/MM) method, we investigated a proton-transfer (PT) process taking place in CaRP obelin from Obelia longissima for emitter formation. Our calculations demonstrate a concerted PT process with a water molecule as a bridge between anionic S-1-coelenteramide and the nearest histidine residue. The low activation barrier as well as the strong hydrogen-bond network between the proton donor and the proton acceptor suggests a fast PT process comparable with that of the lifetime of excited anionic S-1-coelenteramide. The existence of the PT process eliminates the discrepancy between experimental and theoretical studies. The fast PT process at emitter formation can also take place in other CaRPs.

WOS
Держатели документа:
Shanghai Inst Technol, Sch Chem & Environm Engn, Shanghai 201418, Peoples R China.
Fed Res Ctr Krasnoyarsk Sci Ctr SB RAS, Inst Biophys SB RAS, Photo Biol Lab, Krasnoyarsk 660036, Russia.
Beijing Normal Univ Zhuhai, Ctr Adv Mat Res, Adv Inst Nat Sci, Zhuhai 519087, Peoples R China.
Beijing Normal Univ, Coll Chem, Key Lab Theoret & Computat Photochem, Minist Educ, Beijing 100875, Peoples R China.

Доп.точки доступа:
Chen, Shu-Feng; Vysotski, Eugene S.; Liu, Ya-Jun; Vysotski, Eugene; Program of Shanghai Institute of Technology [YJ2016-42]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21973005, 21911530094]; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [20-04-00085, 19-14-53004]

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17.

Thermal Inactivation of Butyrylcholinesterase in Starch and Gelatin Gels / V. I. Lonshakova-Mukina, E. N. Esimbekova, V. A. Kratasyuk // Catalysts. - 2021. - Vol. 11, Is. 4. - Ст. 492, DOI 10.3390/catal11040492. - Cited References:39. - The research was funded by the Government of Krasnoyarsk Territory, Krasnoyarsk Regional Fund of Science, and the Russian Foundation for Basic Research [project No 20-44-242001]. . - ISSN 2073-4344

РУБ Chemistry, Physical

Кл.слова (ненормированные):
butyrylcholinesterase -- thermal inactivation -- enzyme stability -- kinetics -- starch -- gelatin
Аннотация: The present study demonstrates a simple approach to enhancing thermal stability of butyrylcholinesterase (BChE) by using natural polymers. Analysis of thermal inactivation of the tetrameric BChE in starch and gelatin gels at 50-64 degrees C showed that thermal inactivation followed second-order kinetics and involved two alternating processes of BChE inactivation, which occurred at different rates (fast and slow processes). The activation enthalpy Delta H-# and the activation entropy Delta S-# for BChE in starch and gelatin gels were evaluated. The values of Delta H-# for the fast and the slow thermal inactivation of BChE in starch gel were 61 +/- 3, and 22 +/- 2 kcal/mol, respectively, and the values of Delta S-# were 136 +/- 12 and -2.03 +/- 0.05 cal center dot K-1 center dot mol(-1), respectively. Likewise, the values of Delta H-# for BChE in gelatin gel were 58 +/- 6 and 109 +/- 11 kcal/mol, and the values of Delta S-# were 149 +/- 16 and 262 +/- 21 cal center dot K-1 center dot mol(-1), respectively. The values of the activation parameters obtained in this study suggest that starch gel produced a stronger stabilizing effect on BChE exposed to elevated temperatures over long periods compared with gelatin gel.

WOS
Держатели документа:
Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Biophys Dept, Krasnoyarsk 660041, Russia.
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Lonshakova-Mukina, Victoria I.; Esimbekova, Elena N.; Kratasyuk, Valentina A.; Government of Krasnoyarsk Territory; Krasnoyarsk Regional Fund of Science; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [20-44-242001]

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18.

Humic Substances Mitigate the Impact of Tritium on Luminous Marine Bacteria. Involvement of Reactive Oxygen Species / T. V. Rozhko, O. V. Kolesnik, G. A. Badun [et al.] // Int. J. Mol. Sci. - 2020. - Vol. 21, Is. 18. - Ст. 6783, DOI 10.3390/ijms21186783. - Cited References:74. - This work was supported by RFBR-Krasnoyarsk Regional Foundation N 18-44-242002, 18-44-240004. . - ISSN 1422-0067

РУБ Biochemistry & Molecular Biology + Chemistry, Multidisciplinary
Рубрики:
IONIZING-RADIATION
OXIDATIVE STRESS
DETOXIFICATION PROCESSES
Кл.слова (ненормированные):
tritium -- humic substances -- luminous marine bacterium -- bioassay -- detoxification -- reactive oxygen species -- toxicity -- adaptive response -- hormesis
Аннотация: The paper studies the combined effects of beta-emitting radionuclide tritium and Humic Substances (HS) on the marine unicellular microorganism-luminous bacteria-under conditions of low-dose radiation exposures (<0.04 Gy). Tritium was used as a component of tritiated water. Bacterial luminescence intensity was considered as a tested physiological parameter. The bioluminescence response of the marine bacteria to tritium corresponded to the "hormesis" model: it included stages of bioluminescence inhibition and activation, as well as the absence of the effect. HS were shown to decrease the inhibition and activation effects of tritium, similar to those of americium-241, alpha-emitting radionuclide, studied earlier. Correlations between the bioluminescence intensity and the content of Reactive Oxygen Species (ROS) were found in the radioactive bacterial suspensions. The results demonstrate an important role of HS in natural processes in the regions of low radioactive contamination: HS can mitigate radiotoxic effects and adaptive response of microorganisms to low-dose radioactive exposures. The involvement of ROS in these processes was demonstrated.

WOS
Держатели документа:
Krasnoyarsk State Med Acad, Krasnoyarsk 660022, Russia.
RAS, Inst Biophys, Fed Res Ctr Krasnoyarsk Sci Ctr, SB, Krasnoyarsk 660036, Russia.
Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119991, Russia.
Irkutsk State Univ, Biol Dept, Irkutsk 664003, Russia.
Siberian Fed Univ, Dept Biophys, Svobodny 79, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Rozhko, Tatiana V.; Kolesnik, Olga V.; Badun, Gennadii A.; Stom, Devard I.; Kudryasheva, Nadezhda S.; Kudryasheva, Nadezhda; RFBR-Krasnoyarsk Regional Foundation [N 18-44-242002, 18-44-240004]

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19.

Humic substances mitigate the impact of tritium on luminous marine bacteria. Involvement of reactive oxygen species / T. V. Rozhko, O. V. Kolesnik, G. A. Badun [et al.] // International Journal of Molecular Sciences. - 2020. - Vol. 21, Is. 18. - Ст. 6783. - P1-12, DOI 10.3390/ijms21186783 . - ISSN 1661-6596
Кл.слова (ненормированные):
Adaptive response -- Bioassay -- Detoxification -- Hormesis -- Humic substances -- Luminous marine bacterium -- Reactive oxygen species -- Toxicity -- Tritium
Аннотация: The paper studies the combined effects of beta-emitting radionuclide tritium and Humic Substances (HS) on the marine unicellular microorganism—luminous bacteria—under conditions of low-dose radiation exposures (<0.04 Gy). Tritium was used as a component of tritiated water. Bacterial luminescence intensity was considered as a tested physiological parameter. The bioluminescence response of the marine bacteria to tritium corresponded to the “hormesis” model: it included stages of bioluminescence inhibition and activation, as well as the absence of the effect. HS were shown to decrease the inhibition and activation effects of tritium, similar to those of americium-241, alpha-emitting radionuclide, studied earlier. Correlations between the bioluminescence intensity and the content of Reactive Oxygen Species (ROS) were found in the radioactive bacterial suspensions. The results demonstrate an important role of HS in natural processes in the regions of low radioactive contamination: HS can mitigate radiotoxic effects and adaptive response of microorganisms to low-dose radioactive exposures. The involvement of ROS in these processes was demonstrated. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Scopus
Держатели документа:
Krasnoyarsk State Medical Academy, Krasnoyarsk, 660022, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, Krasnoyarsk, 660036, Russian Federation
Department of Chemistry, Moscow State University, Moscow, 119991, Russian Federation
Biology Department, Irkutsk State University, Irkutsk, 664003, Russian Federation
Biophysics Department, Siberian Federal University, Svobodny 79, Krasnoyarsk, 660041, Russian Federation

Доп.точки доступа:
Rozhko, T. V.; Kolesnik, O. V.; Badun, G. A.; Stom, D. I.; Kudryasheva, N. S.

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20.

Redquorinxs mutants with enhanced calcium sensitivity and bioluminescence output efficiently report cellular and neuronal network activities / A. Bakayan, S. Picaud, N. P. Malikova [et al.] // Int. J. Mol. Sci. - 2020. - Vol. 21, Is. 21. - Ст. 7846. - P1-22, DOI 10.3390/ijms21217846 . - ISSN 1661-6596
Кл.слова (ненормированные):
Aequorin -- Bioluminescence -- BRET -- Calcium sensor -- GPCR assay -- Mutagenesis -- Neuronal network imaging
Аннотация: Considerable efforts have been focused on shifting the wavelength of aequorin Ca2+? dependent blue bioluminescence through fusion with fluorescent proteins. This approach has notably yielded the widely used GFP?aequorin (GA) Ca2+ sensor emitting green light, and tdTomato-aequorin (Redquorin), whose bioluminescence is completely shifted to red, but whose Ca2+ sensitivity is low. In the present study, the screening of aequorin mutants generated at twenty?four amino acid positions in and around EF?hand Ca2+?binding domains resulted in the isolation of six aequorin single or double mutants (AequorinXS) in EF2, EF3, and C?terminal tail, which exhibited markedly higher Ca2+ sensitivity than wild?type aequorin in vitro. The corresponding Redquorin mutants all showed higher Ca2+ sensitivity than wild?type Redquorin, and four of them (RedquorinXS) matched the Ca2+ sensitivity of GA in vitro. RedquorinXS mutants exhibited unaltered thermostability and peak emission wavelengths. Upon stable expression in mammalian cell line, all RedquorinXS mutants reported the activation of the P2Y2 receptor by ATP with higher sensitivity and assay robustness than wt?Redquorin, and one, RedquorinXS?Q159T, outperformed GA. Finally, wide?field bioluminescence imaging in mouse neocortical slices showed that RedquorinXS?Q159T and GA similarly reported neuronal network activities elicited by the removal of extracellular Mg2+. Our results indicate that RedquorinXS?Q159T is a red light?emitting Ca2+ sensor suitable for the monitoring of intracellular signaling in a variety of applications in cells and tissues, and is a promising candidate for the transcranial monitoring of brain activities in living mice. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Scopus
Держатели документа:
Institut de Neurobiologie Alfred Fessard, UPR 3294, Centre National de la Recherche Scientifique (CNRS), Avenue de la Terrasse, Gif?sur?Yvette, 91198, France
BioEmergences Unit, CNRS USR 3695, Universite Paris?Saclay, Avenue de la Terrasse, Gif?sur?Yvette, 91198, France
Neuroscience Paris Seine ? Institut de Biologie Paris Seine (NPS ? IBPS), CNRS, UMR8246, INSERM U1130, Sorbonne Universite UM119, Paris, 75005, France
Photobiology Laboratory, Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Bakayan, A.; Picaud, S.; Malikova, N. P.; Tricoire, L.; Lambolez, B.; Vysotski, E. S.; Peyrieras, N.

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