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Volatile metabolites of higher plant crops as a photosynthesizing life support system component under temperature stress at different light intensities / I. I. Gitelson [et al.] // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1781-1786, DOI 10.1016/S0273-1177(03)00121-2 . - ISSN 0273-1177
Кл.слова (ненормированные):
Composition -- Crops -- Heat resistance -- Metabolites -- Photosynthesis -- Volatile metabolites -- Space research -- biosphere -- article -- comparative study -- gas -- growth, development and aging -- heat -- indoor air pollution -- light -- metabolism -- microclimate -- photon -- photosynthesis -- physiology -- radiation exposure -- volatilization -- wheat -- Air Pollution, Indoor -- Environment, Controlled -- Gases -- Heat -- Life Support Systems -- Light -- Photons -- Photosynthesis -- Triticum -- Volatilization
Аннотация: The effect of elevated temperatures of 35 and 45В°C (at the intensities of photosynthetically active radiation 322, 690 and 1104 ?mol-m-2-s-1) on the photosynthesis, respiration, and qualitative and quantitative composition of the volatiles emitted by wheat (Triticum aestuvi L., cultivar 232) crops was investigated in growth chambers. Identification and quantification of more than 20 volatile compounds (terpenoids - ?-pinene, ?3 carene, limonene, benzene, ?-and transcaryophyllene, ?- and ?-terpinene, their derivatives, aromatic hydrocarbons, etc.) were conducted by gas chromatograph/mass spectrometry. Under light intensity of 1104 ?mol-m-2-s-1, heat resistance of photosynthesis and respiration increased at 35В°C and decreased at 45В°C. The action of elevated temperatures brought about variations in the rate and direction of the synthesis of volatile metabolites. The emission of volatile compounds was the greatest under a reduced irradiation of 322 ?mol-m-2-s-1 and the smallest under 1104 ?mol-m-2-s-1 at 35В°C. During the repair period, the contents and proportions of volatile compounds were different from their initial values, too. The degree of disruption and the following recovery of the functional state depended on the light intensity during the exposure to elevated temperatures. The investigation of the atmosphere of the growth chamber without plants has revaled the substances that were definitely technogenic in origin: tetramethylurea, dimethylsulfide, dibutylsulfide, dibutylphthalate, and a number of components of furan and silane nature. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, I.I.; Tikhomirov, A.A.; Parshina, O.V.; Ushakova, S.A.; Kalacheva, G.S.

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Volatile metabolites and external CO2 exchange of wheat cenoses under optimal conditions and thermal stress / I. I. Gitel'son [et al.] // Applied Biochemistry and Microbiology. - 2002. - Vol. 38, Is. 1. - P78-82, DOI 10.1023/A:1013212907872 . - ISSN 0003-6838
Кл.слова (ненормированные):
carbon dioxide -- volatile agent -- article -- biosynthesis -- carbon dioxide transport -- chemical composition -- concentration (parameters) -- controlled study -- cultivar -- metabolite -- nonhuman -- photosynthesis -- photosynthetically active radiation -- plant metabolism -- qualitative analysis -- quantitative analysis -- stress -- temperature sensitivity -- thermal exposure -- thermostability -- wheat -- Rickettsia sp. PAR -- Triticum -- Triticum aestivum
Аннотация: The effects of elevated temperature (35 and 45В°C) on photosynthesis, respiration, and both the qualitative and quantitative compositions of volatile emissions (VE) of wheat (Triticum aestuvum L. cultivar 232) cenoses at light intensities of 70, 150, or 240 W/m2 of photosynthetically available radiation (PAR) were studied. At a PAR of 240 W/m2, the thermal stabilities of photosynthesis and respiration increased at 35В°C and decreased at 45В°C. Elevated temperatures nonuniformly changed the rates and direction of VE syntheses. In this process, the highest increase in VE evolution was observed at 70 W/m2 and 35В°C; the lowest, at 240 W/m2. In addition, the concentrations and composition of VE during the repair period differed from the initial values.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitel'son, I.I.; Tikhomirov, A.A.; Parshina, O.V.; Ushakova, S.A.; Kalacheva, G.S.

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Use of the aquatic plant Elodea canadensis to assess toxicity and genotoxicity of Yenisei River sediments / T. A. Zotina [et al.] // Environ. Toxicol. Chem. - 2015. - Vol. 34, Is. 10. - P2310-2321, DOI 10.1002/etc.3057 . - ISSN 0730-7268
Кл.слова (ненормированные):
Aquatic plants -- Biomarkers -- Genotoxicity -- Sediment quality -- Sediment toxicity -- Biomarkers -- Chromosomes -- Cytotoxicity -- Pollution -- Pollution control -- Radioactive waste disposal -- Radioactivity -- River pollution -- Sediments -- Toxicity -- Aquatic plants -- Genotoxicities -- Laboratory bioassay -- Radioactive contamination -- Radioactive pollution -- Sediment quality -- Sediment toxicity -- Toxicity endpoints -- Rivers -- Article -- bioassay -- controlled study -- cytotoxicity -- Elodea canadensis -- environmental exposure -- genotoxicity -- indicator organism -- lake sediment -- mitosis index -- nonhuman -- plant growth -- plant root -- priority journal -- radioactive pollution -- river -- root length -- Russian Federation -- sensitivity analysis -- shoot -- toxicity testing -- Elodea -- Elodea canadensis
Аннотация: The toxicity, cytotoxicity, and genotoxicity of bulk sediments from the Yenisei River (Siberia, Russia) were estimated in laboratory bioassays based on several endpoints in the aquatic plant Elodea canadensis. The bottom sediment samples were collected in the Yenisei River upstream and downstream of the sources of chemical and radioactive contamination. The testing revealed different sensitivities of Elodea endpoints to the quality of the bottom sediment: weight of shoots
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Держатели документа:
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Zotina, T. A.; Trofimova, E. A.; Medvedeva, M. Y.; Dementyev, D. V.; Bolsunovsky, A. Y.

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Untangling metabolic and spatial interactions of stress tolerance in plants. 1. Patterns of carbon metabolism within leaves / K. Y. Biel [et al.] // Protoplasma. - 2010. - Vol. 245, Is. 1. - P49-73, DOI 10.1007/s00709-010-0135-7 . - ISSN 0033-183X
Кл.слова (ненормированные):
Carbon metabolism -- Leaf anatomy -- Leaf form and function -- Maximal ecological utility -- Photosynthesis -- Stress tolerance Spinacia oleracea -- aspartate aminotransferase isoenzyme 1 -- bicarbonate -- carbon -- carbon dioxide -- catalase -- chlorophyll -- malate dehydrogenase -- oxygen -- ribulosebisphosphate carboxylase -- vegetable protein -- article -- enzymology -- histology -- light -- metabolism -- oxidation reduction reaction -- photosynthesis -- physiological stress -- physiology -- plant leaf -- spinach -- theoretical model -- Aspartate Aminotransferase, Cytoplasmic -- Bicarbonates -- Carbon -- Carbon Dioxide -- Catalase -- Chlorophyll -- Light -- Malate Dehydrogenase -- Models, Theoretical -- Oxidation-Reduction -- Oxygen -- Photosynthesis -- Plant Leaves -- Plant Proteins -- Ribulose-Bisphosphate Carboxylase -- Spinacia oleracea -- Stress, Physiological -- Spinacia oleracea
Аннотация: The localization of the key photoreductive and oxidative processes and some stress-protective reactions within leaves of mesophytic C3 plants were investigated. The role of light in determining the profile of Rubisco, glutamate oxaloacetate transaminase, catalase, fumarase, and cytochrome-c-oxidase across spinach leaves was examined by exposing leaves to illumination on either the adaxial or abaxial leaf surfaces. Oxygen evolution in fresh paradermal leaf sections and CO2 gas exchange in whole leaves under adaxial or abaxial illumination was also examined. The results showed that the palisade mesophyll is responsible for the midday depression of photosynthesis in spinach leaves. The photosynthetic apparatus was more sensitive to the light environment than the respiratory apparatus. Additionally, examination of the paradermal leaf sections by optical microscopy allowed us to describe two new types of parenchyma in spinach-pirum mesophyll and pillow spongy mesophyll. A hypothesis that oxaloacetate may protect the upper leaf tissue from the destructive influence of active oxygen is presented. The application of mathematical modeling shows that the pattern of enzymatic distribution across leaves abides by the principle of maximal ecological utility. Light regulation of carbon metabolism across leaves is discussed. В© 2010 Springer-Verlag.

Scopus
Держатели документа:
Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russian Federation
Biosphere Systems International Foundation, Oro Valley, AZ 85755, United States
International Scientific Centre for Organism Extreme States Research, Krasnoyarsk Scientific Centre, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation
Institute of Forest, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation
Biocompatible Plant Research Institute, College of Natural Sciences, California State University, Chico, CA 95929-0555, United States : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Biel, K.Y.; Fomina, I.R.; Nazarova, G.N.; Soukhovolsky, V.G.; Khlebopros, R.G.; Nishio, J.N.

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Tritium in surface waters of the Yenisei River basin / A. Y. Bolsunovsky, L. G. Bondareva // Journal of Environmental Radioactivity. - 2003. - Vol. 66, Is. 3. - P285-294, DOI 10.1016/S0265-931X(02)00132-7 . - ISSN 0265-931X
Кл.слова (ненормированные):
Deep-well injection of radionuclides -- Nuclear reactor -- Surface and ground waters -- Tritium -- Yenisei River basin -- Cooling water -- Nuclear reactors -- Sediments -- Surface waters -- River basins -- Tritium -- carbon 14 -- surface water -- tritium -- groundwater -- nuclear power plant -- radioactive pollution -- surface water -- tritium -- article -- catchment -- measurement -- radioactive contamination -- river -- sediment -- Environmental Monitoring -- Geologic Sediments -- Mining -- Power Plants -- Reference Values -- Russia -- Tritium -- Water Pollutants, Radioactive -- Russian Federation -- Tritium
Аннотация: This paper reports an investigation of the tritium content in the surface waters of the Yenisei River basin near the Mining and Chemical Combine (MCC). In 2001 the maximum tritium concentration in the Yenisei River did not exceed 4 В± 1 Bq l-1, which is consistent with the data of 1998-99. However, it has been found that there are surface waters containing enhanced tritium as compared with the background values for the Yenisei River. For instance, in the Ploskii Stream and the Shumikha River the maximum tritium concentrations amount to 168 and 81 Bq l-1, respectively. The source of tritium in these surface waters is the last operating reactor at the MCC, which still uses the Yenisei water as coolant. In water and sediment samples of the Bolshaya Tel River (a tributary of the Yenisei River) the tritium content turned out to be at least 10 times higher than the background values for the Yenisei River. The measurements conducted at the RPA RADON (Moscow) revealed not only tritium but also the artificial radionuclide 14C in the Bolshaya Tel samples. The data obtained suggest that the Bolshaya Tel River receives the major part of tritium from sediments rather than from the water catchment area. This allows the conclusion that there is water exchange between the surface waters and the radioactively contaminated underground horizons of the "Severny" testing site. В© 2002 Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsky, A.Y.; Bondareva, L.G.

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Trends in microevolution of microbial populations in open systems / A. V. Brilkov [et al.] // Doklady Biochemistry and Biophysics. - 2005. - Vol. 404, Is. 1-6. - P349-352, DOI 10.1007/s10628-005-0111-x . - ISSN 1607-6729
Кл.слова (ненормированные):
article -- bacterial phenomena and functions -- bacterium -- biological model -- culture technique -- Escherichia coli -- evolution -- genetics -- growth, development and aging -- mathematics -- methodology -- mutation -- nanotechnology -- pH -- physiology -- population dynamics -- time -- Bacteria -- Bacterial Physiology -- Cell Culture Techniques -- Escherichia coli -- Evolution -- Hydrogen-Ion Concentration -- Mathematics -- Models, Biological -- Mutation -- Nanotechnology -- Population Dynamics -- Time Factors

Scopus
Держатели документа:
Krasnoyarsk State University, Krasnoyarsk, 660062, Russian Federation
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Brilkov, A.V.; Loginov, I.A.; Morozova, E.V.; Pechurkin, N.S.

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Transfer of xenobiotics through cell membranes of luminous bacteria / S. E. Medvedeva // Luminescence. - 1999. - Vol. 14, Is. 5. - P267-270 . - ISSN 1522-7235
Кл.слова (ненормированные):
Luminous bacteria -- Toxicant -- Ultrastructure -- bacterial DNA -- edetic acid -- toluene -- xenobiotic agent -- article -- cell membrane -- DNA damage -- drug effect -- luminescence -- metabolism -- Photobacterium -- sensitivity and specificity -- transport at the cellular level -- ultrastructure -- Vibrio -- Biological Transport -- Cell Membrane -- DNA Damage -- DNA, Bacterial -- Edetic Acid -- Luminescence -- Photobacterium -- Sensitivity and Specificity -- Toluene -- Vibrio -- Xenobiotics
Аннотация: The influence of some chemical substances on luminous bacteria was studied to elucidate the interrelation between the xenobiotics action on bacterial luminescence and cell ultrastructure. Such substances as quinones, phenols, chlorides of heavy metals (in concentrations of substances inhibiting luminescence by 50%) resulted in damaging effects upon bacteria: a lot of cells had damage of membranes due to changes in their permeability. It was found that the high concentration of EDTA and toluene decreased the luminescence and caused the condensation of DNA-fibrils and the cell damage after long-term and short-term action. The low concentration of EDTA and toluene did not decrease the bacterial luminescence; the noticeable damage of cell membranes did not take place during short-term treatment. However, the long action of these substances changed the membrane permeability resulting in increased sensitivity of bacterial luminescence to some toxic substances. Copyright В© 1999 John Wiley & Sons, Ltd.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Medvedeva, S.E.

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Transfer of essential polyunsaturated fatty acids from an aquatic to terrestrial ecosystem through the fish-bird trophic pair / M. I. Gladyshev [et al.] // Doklady Biological Sciences. - 2010. - Vol. 431, Is. 1. - P121-123, DOI 10.1134/S0012496610020146 . - ISSN 0012-4966
Кл.слова (ненормированные):
fatty acid -- fresh water -- triacylglycerol -- unsaturated fatty acid -- animal -- article -- biological model -- biomass -- bird -- chemistry -- ecosystem -- fish -- food chain -- metabolism -- muscle -- pathology -- physiology -- species difference -- Animals -- Biomass -- Birds -- Ecosystem -- Fatty Acids -- Fatty Acids, Unsaturated -- Fishes -- Food Chain -- Fresh Water -- Models, Biological -- Muscles -- Species Specificity -- Triglycerides -- Aves

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation
Papanin Institute of Inland Water Biology, Russian Academy of Sciences, Borok, Yaroslavl' oblast, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gladyshev, M.I.; Krylov, A.V.; Sushchik, N.N.; Malin, M.I.; Makhutova, O.N.; Chalova, I.V.; Kalacheva, G.S.

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Tolerance of LSS plant component to elevated temperatures / S. A. Ushakova, A. A. Tikhomirov // Acta Astronautica. - 2002. - Vol. 50, Is. 12. - P759-764, DOI 10.1016/S0094-5765(02)00010-3 . - ISSN 0094-5765
Кл.слова (ненормированные):
Metabolism -- Photosynthesis -- Rate constants -- Thermal effects -- Thermoanalysis -- Thermal tolerance -- Life support systems (spacecraft) -- carbon dioxide -- adaptation -- article -- Brassicaceae -- comparative study -- heat -- instrumentation -- light -- metabolism -- microclimate -- photosynthesis -- physiology -- plant physiology -- wheat -- Adaptation, Physiological -- Brassicaceae -- Carbon Dioxide -- Ecological Systems, Closed -- Heat -- Life Support Systems -- Light -- Photosynthesis -- Plant Physiology -- Triticum
Аннотация: Stability of LSS based on biological regeneration of water, air and food subject to damaging factors is largely dependent on the behavior of the photosynthesizing component represented, mainly, by higher plants. The purpose of this study is to evaluate the tolerance of uneven-aged wheat and radish cenoses to temperature effects different in time and value. Estimation of thermal tolerance of plants demonstrated that exposure for 20 h to the temperature increasing to 45В°C brought about irreversible damage both in photosynthetic processes (up to 80% of initial value) and the processes of growth and development. Kinetics of visible photosynthesis during exposure to elevated temperatures can be used to evaluate critical exposure time within the range of which the damage of metabolic processes is reversible. With varying light intensity and air temperature it is possible to find a time period admissible for the plants to stay under adverse conditions without considerable damage of metabolic processes. В© 2002 Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics (Russian Academy of Sciences, Siberian Branch) Academgorodok, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Ushakova, S.A.; Tikhomirov, A.A.

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10.

Tissue response to the implantation of biodegradable polyhydroxyalkanoate sutures / E. I. Shishatskaya [et al.] // Journal of Materials Science: Materials in Medicine. - 2004. - Vol. 15, Is. 6. - P719-728, DOI 10.1023/B:JMSM.0000030215.49991.0d . - ISSN 0957-4530
Кл.слова (ненормированные):
Necrosis -- Polymeric implants -- Suppurative inflammation -- Tissue reaction -- Biodegradation -- Calcification (biochemistry) -- Cells -- Copolymers -- Implants (surgical) -- Silk -- Tissue -- Tumors -- Materials science -- acid phosphatase -- copolymer -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- polyhydroxyvaleric acid -- unclassified drug -- animal experiment -- animal model -- article -- biodegradable implant -- blood vessel reactivity -- catgut -- controlled study -- enzyme activity -- female -- giant cell -- histochemistry -- inflammation -- macrophage -- nonhuman -- priority journal -- rat -- silk -- suture -- tensile strength -- tissue reaction -- tissue structure -- wound healing -- young modulus -- Absorbable Implants -- Animals -- Female -- Fibrosis -- Foreign-Body Reaction -- Hydroxybutyrates -- Muscle, Skeletal -- Polyesters -- Polymers -- Rats -- Rats, Wistar -- Sutures -- Treatment Outcome -- Wounds, Penetrating -- Animalia
Аннотация: Polyhydroxyalkanoate (PHA) sutures were implanted to test animals intramuscularly, and tissue reaction was investigated and compared with the reaction to silk and catgut. Tested monofilament sutures made of PHAs of two types polyhydroxybutyrate (PHB) and a copolymer of hydroxybutyrate and hydroxyvalerate (PHV) featured the strength necessary for the healing of muscle-fascial wounds. The reaction of tissues to polymeric implants was similar to their reaction to silk and was less pronounced than the reaction to catgut; it was expressed in a transient post-traumatic inflammation (up to four weeks) and the formation of a fibrous capsule less than 200 ?m thick, which became as thin as 4060 ?m after 16 weeks, in the course of reverse development. Macrophages and foreign-body giant cells with a high activity of acid phosphatase were actively involved in this process. PHB and PHB/PHV sutures implanted intramuscularly for an extended period (up to one year) did not cause any acute vascular reaction at the site of implantation or any adverse events, such as suppurative inflammation, necrosis, calcification of the fibrous capsule or malignant tumor formation. No statistically significant differences were revealed in the tissue response to polymer sutures of the two types. Capsules around silk and catgut sutures did not become significantly thinner.

Scopus
Держатели документа:
Inst. Biophys. Siberian Br. Russ. A., Akademgorodok, Krasnoyarsk 660036, Russia, Russian Federation
Terr. Pathological Anatomy Bureau, Partisan Zheleznyak St. 1, Krasnoyarsk 660049, Russia, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Puzyr, A.P.; Mogilnaya, O.A.; Efremov, S.N.

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11.

Tissue response to biodegradable suture threads made of polyoxyalkanoates / E. I. Shishatskaya [и др.] // Meditsinskaya Tekhnika. - 2002. - Is. 4. - С. 23-29 . - ISSN 0025-8075
Кл.слова (ненормированные):
Biocompatibility -- Biodegradation -- Implants (surgical) -- Materials testing -- Organic polymers -- Polyoxyalkanoates -- Suture threads -- Biomaterials -- biomaterial -- polymer -- animal -- article -- bioremediation -- female -- metabolism -- rat -- suture -- Wistar rat -- Animals -- Biocompatible Materials -- Biodegradation, Environmental -- Female -- Polymers -- Rats -- Rats, Wistar -- Sutures
Аннотация: The paper presents data on the materials and methods of obtaining suture threads based on of the polymer batty acid derivatives (polyoxyalkanoates). Results of the study of tissue response to biodegradable polyoxyalkanoate suture threads in the postoperative period are analyzed. These suture threads have been shown to have the required strength and meet a number of other requirements for currently available materials.

Scopus
Держатели документа:
Inst. Biofiziki SO RAN, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Efremova, S.N.; Puzyr, S.P.; Mogil'naya, O.A.

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12.

Tissue response to biodegradable suture threads made of polyhydroxyalkanoates / E. I. Shishatskaya [et al.] // Biomedical Engineering. - 2002. - Vol. 36, Is. 4. - P210-217, DOI 10.1023/A:1021184119268 . - ISSN 0006-3398
Кл.слова (ненормированные):
acid phosphatase -- alkaline phosphatase -- polyhydroxyalkanoic acid -- animal experiment -- animal tissue -- article -- biocompatibility -- biodegradability -- controlled study -- elasticity -- enzyme activity -- enzyme mechanism -- female -- histochemistry -- incision -- nonhuman -- physical chemistry -- postoperative period -- rat -- rigidity -- suture -- thickness -- tissue reaction -- wound healing -- Animalia

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Krasnoyarsk Terr. Bur. Pathol. Anat., Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Efremov, S.N.; Puzyr', A.P.; Mogil'Naya, O.A.

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13.

Tissue reaction to intramuscular injection of resorbable polymer microparticles / E. I. Shishatskaya [et al.] // Bulletin of Experimental Biology and Medicine. - 2007. - Vol. 144, Is. 6. - P786-790, DOI 10.1007/s10517-007-0432-0 . - ISSN 0007-4888
Кл.слова (ненормированные):
Microencapsulation -- Polyhydroxybutyrate -- Resorbable polymers -- Tissue reaction -- poly(3 hydroxybutyric acid) -- polymer -- animal experiment -- animal tissue -- article -- cell infiltration -- controlled study -- drug delivery system -- drug formulation -- female -- giant cell -- inflammation -- macrophage -- microencapsulation -- nonhuman -- rat -- tissue reaction -- Absorbable Implants -- Animals -- Drug Compounding -- Female -- Foreign-Body Reaction -- Injections, Intramuscular -- Microspheres -- Polyesters -- Rats -- Rats, Wistar
Аннотация: Tissue reaction to implantation of polymeric microparticles from resorbable polymer (polyhydroxybutyrate) is characterized by slight inflammatory reaction and pronounced progressive macrophage infiltration with the presence of mono-and multinuclear foreign body giant cells resorbing the polymeric matrix. No fibrous capsules were formed around the polymeric microparticles; neither necrosis nor other adverse morphological changes and tissue transformation in response to implantation of the PHB microparticles were recorded. The results indicate good prospects of using polyhydroxybutyrate for the construction of long-acting dosage forms as microparticles for intramuscular injection. В© Springer Science+Business Media, Inc. 2007.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division of Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Voinova, O.N.; Goreva, A.V.; Mogilnaya, O.A.; Volova, T.G.

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14.

Tissue morphogenesis under the conditions of implantation of polyhydroxybutyrate, a biodegradable polymer. / E. I. Shishatskaya [et al.] // Doklady Biological Sciences. - 2002. - Vol. 383, Is. 1-6. - P123-126 . - ISSN 0012-4966
Кл.слова (ненормированные):
biomaterial -- hydroxybutyric acid -- polymer -- animal -- article -- bioremediation -- female -- morphogenesis -- rat -- Wistar rat -- Animals -- Biocompatible Materials -- Biodegradation, Environmental -- Female -- Hydroxybutyrates -- Morphogenesis -- Polymers -- Rats -- Rats, Wistar

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Puzyr, A.P.; Mogil'naya, O.A.; Efremov, S.N.; Gitelson, I.I.

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15.

Time course of the spectral brightness of agricultural crops during the vegetation period in Krasnoyarsk krai / A. F. Sid'ko, I. Yu. Pugacheva, A. P. Shevyrnogov // Doklady Biological Sciences. - 2008. - Vol. 419, Is. 1. - P114-117, DOI 10.1134/S0012496608020130 . - ISSN 0012-4966
Кл.слова (ненормированные):
article -- crop -- histology -- light -- metabolism -- plant -- Russian Federation -- season -- spectroscopy -- Crops, Agricultural -- Light -- Plants -- Russia -- Seasons -- Spectrum Analysis

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sid'ko, A.F.; Pugacheva, I.Yu.; Shevyrnogov, A.P.

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16.

Theoretical and experimental decisions in the creation of an artificial ecosystem for human life support in space. / L. V. Kirensky [et al.] // Life sciences and space research. - 1971. - Vol. 9. - P75-80 . - ISSN 0075-9422
Кл.слова (ненормированные):
article -- bacterium -- biotechnology -- Chlorella -- energy metabolism -- human -- instrumentation -- man machine interaction -- metabolism -- microclimate -- space flight -- weightlessness -- Bacteria -- Biotechnology -- Chlorella -- Ecological Systems, Closed -- Energy Metabolism -- Environment, Controlled -- Humans -- Life Support Systems -- Man-Machine Systems -- Space Flight -- Weightlessness
Аннотация: All of man's former space flights were not real ventures into space in the biological sense, as his life was supported with unregenerated earth supplies. The coming stage of space exploration requires man's long existence in the cosmos and on the other planets. This stage of man's activity outside the earth become possible only by creating small man-made ecosystems, permitting the support of his metabolism by the recycling of substances of the terrestrial biosphere. Creation of such systems is a new scientific and technical task. Man-made ecosystems are a new product of man's activity, which have no complete analogy, either in nature, or in technology. Stochastic mechanisms, which stabilize biogeocenosis, cannot be effective in small ecosystems. A technique of parametric control over biosynthesis made it possible to calculate, and put to practice, an ecosystem for man with a cyclic regeneration of the atmosphere, water and, partially, food. The specific bio-technological properties of small man-made ecosystems are being analysed. The possibility of their application for man's excursions into space and for the settlement of other planets is being considered.

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Держатели документа:
Institute of Physics, Akademgorodok, Krasnoyarsk, USSR. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kirensky, L.V.; Gitelson, I.I.; Terskov, I.A.; Kovrov, B.G.; Lisovsky, G.M.; Okladnikov, Y.N.

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17.

The yellow bioluminescence bacterium, Vibrio fischeri Y1, contains a bioluminescence active riboflavin protein in addition to the yellow fluorescence FMN protein / V. N. Petushkov, B. G. Gibson, J. Lee // Biochemical and Biophysical Research Communications. - 1995. - Vol. 211, Is. 3. - P774-779, DOI 10.1006/bbrc.1995.1880 . - ISSN 0006-291X
Кл.слова (ненормированные):
riboflavin -- article -- bioluminescence -- fluorescence -- nonhuman -- priority journal -- protein analysis -- protein synthesis -- vibrio -- vibrionaceae -- Bacterial Proteins -- Chromatography, Gel -- Chromatography, Thin Layer -- Flavin Mononucleotide -- Flavoproteins -- Luminescence -- Riboflavin -- Spectrometry, Fluorescence -- Support, U.S. Gov't, P.H.S. -- Vibrio -- Bacteria (microorganisms) -- Photobacterium -- Vibrio -- Vibrio fischeri
Аннотация: The yellow bioluminescence Y1 strain of Vibrio fischeri can produce a 22 kDa protein with either FMN or riboflavin as a bound fluorophore. Both forms are active for shifting the bioluminescence spectral maximum. The fluorescence spectral distribution of the two proteins differs slightly and the in vivo emission appears to be an equal mixture of the two. The bioluminescence activity of the riboflavin Y1 protein contrasts with the inactivity of the related Photobacterium type.

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Держатели документа:
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, United States
Institute of Biophysics, Academy of Sciences of Russia (Siberian Branch), 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Petushkov, V.N.; Gibson, B.G.; Lee, J.

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18.

The use of bioluminescent biotests for study of natural and laboratory aquatic ecosystems / V. A. Kratasyuk [et al.] // Chemosphere. - 2001. - Vol. 42, Is. 8. - P909-915, DOI 10.1016/S0045-6535(00)00177-6 . - ISSN 0045-6535
Кл.слова (ненормированные):
Alcohol dehydrogenase -- Bacterial luciferase -- Bioluminescence -- Blooming -- Pollution -- Trypsin -- Water toxicity -- alcohol dehydrogenase -- benzoquinone -- luciferase -- trypsin -- aquatic ecosystem -- bioluminescence -- water quality -- article -- bacterium culture -- bioluminescence -- blue green alga -- ecosystem -- pond -- seasonal variation -- water pollution -- water quality -- Benzoquinones -- Biological Assay -- Cyanobacteria -- Ecosystem -- Environmental Monitoring -- Eutrophication -- FMN Reductase -- Indicators and Reagents -- Luminescent Measurements -- NADH, NADPH Oxidoreductases -- Water Pollutants -- Russian Federation -- algae -- Bacteria (microorganisms) -- Chlorophyta -- Cyanobacteria -- uncultured cyanobacterium
Аннотация: A set of bioluminescent tests was developed to monitor water quality in natural and laboratory ecosystems. It consisted of four bioluminescent systems: luminous bacteria, coupled enzyme system NADH:FMN-oxidoreductase-luciferase and triplet enzyme systems with alcohol dehydrogenase and trypsin. The set of biotests was applied for a small forest pond (Siberia, Russia), laboratory microecosystems polluted with benzoquinone and a batch culture of blue-green algae. Thereby effects of natural water compared to those of models of heavy pollution and "bloom" of blue-greens on the bioluminescent tests were revealed. The set of biotests was not affected by a natural seasonal variability of water quality in the unpolluted pond, but responded to the heavy pollution and the "bloom" of blue-greens. The set of biotests could be recommended as the alarm test to control the acute toxicity of natural water bodies. В© 2001 Elsevier Science Ltd.

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Держатели документа:
Krasnoyarsk State University, pr. Svobodnii 79, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Krasnoyarsk State Agricultural University, Mira av., 88, Krasnoyarsk, 660049, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kratasyuk, V.A.; Esimbekova, E.N.; Gladyshev, M.I.; Khromichek, E.B.; Kuznetsov, A.M.; Ivanova, E.A.

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19.

The synthesis of hydroxybutyrate and hydroxyvalerate copolymers by the bacterium Ralstonia eutropha / T. G. Volova, G. S. Kalacheva // Mikrobiologiya. - 2005. - Vol. 74, Is. 1. - С. 63-69 . - ISSN 0026-3656
Кл.слова (ненормированные):
?-ketothiolase -- Controlled synthesis -- Poly(hydroxybutyrate-co-hydroxyvalerate) -- Ralstonia eutropha -- Bacteria (microorganisms) -- Cupriavidus necator -- acetoacetyl coenzyme a -- acetyl coenzyme A acyltransferase -- acyl coenzyme A -- acyltransferase -- butyric acid derivative -- carbon dioxide -- fructose -- hydrogen -- poly(3 hydroxybutyrate) co (3 hydroxyvalerate) -- poly(3-hydroxyalkanoic acid) synthase -- poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate) -- polyester -- polyhydroxyalkanoate synthase -- valeric acid derivative -- article -- chemistry -- crystallization -- culture medium -- metabolism -- Wautersia eutropha -- Acetyl-CoA C-Acyltransferase -- Acyl Coenzyme A -- Acyltransferases -- Butyrates -- Carbon Dioxide -- Crystallization -- Culture Media -- Cupriavidus necator -- Fructose -- Hydrogen -- Polyesters -- Valerates
Аннотация: The paper deals with the study of the synthesis of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) copolymers by the bacterium Ralstonia eutropha B-5786 grown under different carbon nutrition conditions (growth on carbon dioxide, fructose, and CO2-valerate and fructose-valerate mixtures). The parameters to be analyzed included the yield of biomass, the yield, synthesis rate, and composition of copolymers, the activity of the key enzymes of polyhydroxyalkanoate (PHA) synthesis (?-ketothiolase, acetoacetyl-CoA reductase, and PHA synthase), the maximum tolerable concentration of valerate to the bacterium, and the conditions that govern the incorporation of hydroxyvalerate to copolymers. This allowed the relationship between cultivation conditions and the proportion of monomers in the copolymers to be deduced. We were able to synthesize a range of 3HB/3HV copolymers and found that the thermal characteristics and the degree of crystallinity of these copolymers depend on the molar fraction of 3HV.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.

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20.

The smallest isoform of Metridia longa luciferase as a fusion partner for hybrid proteins / M. D. Larionova, S. V. Markova, N. V. Tikunova, E. S. Vysotski // Int. J. Mol. Sci. - 2020. - Vol. 21, Is. 14. - Ст. 4971. - P1-16, DOI 10.3390/ijms21144971 . - ISSN 1661-6596
Кл.слова (ненормированные):
Bioluminescence -- Coelenterazine -- Copepod luciferase -- Immunoassay -- Single-chain antibody -- Tick-borne encephalitis virus -- fusion protein -- glycoprotein -- histidine -- messenger RNA -- Metridia longa luciferase -- recombinant protein -- single chain fragment variable antibody -- unclassified drug -- amino terminal sequence -- antibody affinity -- antigen binding -- Article -- binding assay -- binding site -- bioluminescence -- bioluminescence resonance energy transfer -- cross reaction -- dissociation constant -- enzyme activity -- Escherichia coli -- gene -- genetic engineering -- genetic transfection -- immunoassay -- limit of detection -- mluc7 gene -- molecular cloning -- nonhuman -- nucleotide sequence -- protein expression -- protein purification -- protein unfolding -- spectral sensitivity -- tick borne encephalitis -- Tick borne encephalitis virus
Аннотация: Bioluminescent proteins are widely used as reporter molecules in various in vitro and in vivo assays. The smallest isoform of Metridia luciferase (MLuc7) is a highly active, naturally secreted enzyme which, along with other luciferase isoforms, is responsible for the bright bioluminescence of marine copepod Metridia longa. In this study, we report the construction of two variants of a hybrid protein consisting of MLuc7 and 14D5a single-chain antibody to the surface glycoprotein E of tick-borne encephalitis virus as a model fusion partner. We demonstrate that, whereas fusion of a single-chain antibody to either N-or C-terminus of MLuc7 does not affect its bioluminescence properties, the binding site on the single-chain antibody influences its binding capacity. The affinity of 14D5a-MLuc7 hybrid protein (KD = 36.2 nM) where the C-terminus of the single-chain antibody was fused to the N-terminus of MLuc7, appeared to be 2.5-fold higher than that of the reverse, MLuc7-14D5a (KD = 87.6 nM). The detection limit of 14D5a-MLuc7 hybrid protein was estimated to be 45 pg of the recombinant glycoprotein E. Although the smallest isoform of M. longa luciferase was tested as a fusion partner only with a single-chain antibody, it is reasonable to suppose that MLuc7 can also be successfully used as a partner for genetic fusion with other proteins. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Scopus
Держатели документа:
Photobiology Laboratory, Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, 660036, Russian Federation
School of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, 660041, Russian Federation
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russian Federation

Доп.точки доступа:
Larionova, M. D.; Markova, S. V.; Tikunova, N. V.; Vysotski, E. S.

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