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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nechipurenko, Yury D., Semyonov, Denis A., Lavrinenko, Igor A., Lagutkin, Denis A., Generalov, Evgenii A., Zaitceva, Anna Y., Matveeva, Olga, V, Yegorov, Yegor E.
Заглавие : The Role of Acidosis in the Pathogenesis of Severe Forms of COVID-19
Колич.характеристики :15 с
Коллективы : Presidium of the Russian Academy of Sciences for Molecular and Cellular Biology; Program of Fundamental Research for State Academies for years 2013-2020 [01201363818]
Место публикации : Biology-Basel: MDPI, 2021. - Vol. 10, Is. 9. - Ст.852. - ISSN 2079-7737(eISSN), DOI 10.3390/biology10090852
Примечания : Cited References:86. - This research was funded by the Presidium of the Russian Academy of Sciences for Molecular and Cellular Biology and the Program of Fundamental Research for State Academies for years 2013-2020, project no. 01201363818.
Предметные рубрики: RESPIRATORY-ACIDOSIS
LACTATE
COAGULATION
GLYCOLYSIS
SECRETION
Аннотация: Simple Summary Recently, several studies have shown that acidosis, which is increased acidity in the blood and other body tissues, is often associated with severe COVID-19. In this article, we look at the mechanisms and consequences of acidosis that can lead to an exacerbation of COVID-19. We want to draw the attention of readers to the threshold values of such disease characteristics as hypoxia and acidosis, which are associated with a sharp deterioration in the patient's condition. Hypoxia and acidosis mutually reinforce each other according to the principle of a vicious cycle (that is, they are involved in a system of positive feedbacks). Elevated blood lactate (lactic acid) levels are associated with poor clinical outcomes in COVID patients. As a practical recommendation, we propose to pay more attention to the prevention of acidosis, including in the early stages of the disease, when the adjustment of homeostasis requires less effort and is less risky. COVID-19 has specific characteristics that distinguish this disease from many other infections. We suggest that the pathogenesis of severe forms of COVID-19 can be associated with acidosis. This review article discusses several mechanisms potentially linking the damaging effects of COVID-19 with acidosis and shows the existence of a vicious cycle between the development of hypoxia and acidosis in COVID-19 patients. At the early stages of the disease, inflammation, difficulty in gas exchange in the lungs and thrombosis collectively contribute to the onset of acidosis. In accordance with the Verigo-Bohr effect, a decrease in blood pH leads to a decrease in oxygen saturation, which contributes to the exacerbation of acidosis and results in a deterioration of the patient's condition. A decrease in pH can also cause conformational changes in the S-protein of the virus and thus lead to a decrease in the affinity and avidity of protective antibodies. Hypoxia and acidosis lead to dysregulation of the immune system and multidirectional pro- and anti-inflammatory reactions, resulting in the development of a "cytokine storm". In this review, we highlight the potential importance of supporting normal blood pH as an approach to COVID-19 therapy.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prokopkin I., Makhutova O., Kravchuk E., Sushchik N., Anishchenko O., Gladyshev M.
Заглавие : Assessing the reliability of quantitative fatty acid signature analysis and compound-specific isotope analysis-based mixing models for trophic studies
Место публикации : Biomolecules: MDPI, 2021. - Vol. 11, Is. 11. - Ст.1590. - ISSN 2218273X (ISSN), DOI 10.3390/biom11111590
Аннотация: The study of the trophic relationships of aquatic animals requires correct estimates of their diets. We compared the quantitative fatty acid signature analysis (QFASA) and the isotope?mixing model IsoError, based on the compound?specific isotope analysis of fatty acids (CSIA?FA), which are potentially effective models for quantitative diet estimations. In a 21?day experiment, Daphnia was fed a mixture of two food items, Chlorella and Cryptomonas, which were supplied in nearly equal proportions. The percentages and isotope values of the FAs of the algal species and Daphnia were measured. The IsoError based on CSIA?FA gave an estimation of algae consumption using only one FA, 18:3n?3. According to this model, the proportion of consumption of Chlorella decreased while the proportion of consumption of Cryptomonas increased during the experiment. The QFASA model was used for two FA subsets—the extended?dietary subset, which included sixteen FAs, and the dietary one, which included nine FAs. According to both subsets, the portion of consumed Chlorella decreased from Day 5 to 10 and then increased at Day 21. The comparison of the two model approaches showed that the QFASA model is a more reliable method to determine the contribution of different food sources to the diet of zooplankton than the CSIA?based mixing model. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Wu Y. -T., Chiang P. -W., Tandon K., Rogozin D. Y., Degermendzhy A. G., Tang S. -L.
Заглавие : Single-cell genomics-based analysis reveals a vital ecological role of thiocapsa sp. LSW in the meromictic Lake Shunet, Siberia
Место публикации : Microb. Genomics: Microbiology Society, 2021. - Vol. 7, Is. 12. - Ст.000712. - ISSN 20575858 (ISSN), DOI 10.1099/mgen.0.000712
Аннотация: Meromictic lakes usually harbour certain prevailing anoxygenic phototrophic bacteria in their anoxic zone, such as the purple sulfur bacterium (PSB) Thiocapsa sp. LSW (hereafter LSW) in Lake Shunet, Siberia. PSBs have been suggested to play a vital role in carbon, nitrogen and sulfur cycling at the oxic–anoxic interface of stratified lakes; however, the ecological significance of PSBs in the lake remains poorly understood. In this study, we explored the potential ecological role of LSW using a deep-sequencing analysis of single-cell genomics associated with flow cytometry. An approximately 2.7 Mb draft genome was obtained based on the co-assembly of five single-cell genomes. LSW might grow photolithoautotrophically and could play putative roles not only as a carbon fixer and diazotroph, but also as a sulfate reducer/oxidizer in the lake. This study provides insights into the potential ecological role of Thiocapsa sp. in meromictic lakes. © 2021 The Authors.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nechipurenko Y. D., Semyonov D. A., Lavrinenko I. A., Lagutkin D. A., Generalov E. A., Zaitceva A. Y., Matveeva O. V., Yegorov Y. E.
Заглавие : The role of acidosis in the pathogenesis of severe forms of COVID-19
Место публикации : Biology: MDPI, 2021. - Vol. 10, Is. 9. - Ст.852. - ISSN 20797737 (ISSN), DOI 10.3390/biology10090852
Аннотация: COVID-19 has specific characteristics that distinguish this disease from many other infec-tions. We suggest that the pathogenesis of severe forms of COVID-19 can be associated with acidosis. This review article discusses several mechanisms potentially linking the damaging effects of COVID-19 with acidosis and shows the existence of a vicious cycle between the development of hypoxia and acidosis in COVID-19 patients. At the early stages of the disease, inflammation, difficulty in gas exchange in the lungs and thrombosis collectively contribute to the onset of acidosis. In accordance with the Verigo-Bohr effect, a decrease in blood pH leads to a decrease in oxygen saturation, which contributes to the exacerbation of acidosis and results in a deterioration of the patient’s condition. A decrease in pH can also cause conformational changes in the S-protein of the virus and thus lead to a decrease in the affinity and avidity of protective antibodies. Hypoxia and acidosis lead to dysregu-lation of the immune system and multidirectional pro-and anti-inflammatory reactions, resulting in the development of a “cytokine storm”. In this review, we highlight the potential importance of supporting normal blood pH as an approach to COVID-19 therapy. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prudnikova S. V., Evgrafova S. Y., Volova T. G.
Заглавие : Metabolic activity of cryogenic soils in the subarctic zone of Siberia towards “green” bioplastics
Место публикации : Chemosphere: Elsevier Ltd, 2021. - Vol. 263. - Ст.128180. - ISSN 00456535 (ISSN), DOI 10.1016/j.chemosphere.2020.128180
Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic – poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9–11 °C, the total biomass of microorganisms at a 10-20-cm depth was 226–234 mg g?1 soil and CO2 production was 20–46 mg g?1 day?1. The total abundance of microscopic fungi varied between (7.4 ± 2.3) ? 103 and (18.3 ± 2.2) ? 103 CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 ± 0.1) ? 106 CFU g?1 soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique – plating of isolates on the mineral agar with polymer as sole carbon source – was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)-degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. © 2020 Elsevier Ltd
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T., Kiselev E., Nemtsev I., Lukyanenko А., Sukovatyi A., Kuzmin A., Ryltseva G., Shishatskaya E.
Заглавие : Properties of degradable polyhydroxyalkanoates with different monomer compositions
Место публикации : Int. J. Biol. Macromol.: Elsevier B.V., 2021. - Vol. 182. - С. 98-114. - ISSN 01418130 (ISSN), DOI 10.1016/j.ijbiomac.2021.04.008
Аннотация: Purpose: To synthesize and investigate polyhydroxyalkanoates (PHAs) with different monomer composition and percentages and polymer films prepared from them. Results: Various PHAs: homopolymer poly-3-hydroxybutyrate P(3HB) and 2-, 3-, and 4-component copolymers comprising various combinations of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), 4-hydroxybutyrate (4HB), and 3-hydroxyhexanoate (3HHx) monomers were synthesized under specialized conditions. Relationships were found between the monomer composition of PHAs and their molecular-weight and thermal properties and degree of crystallinity. All copolymers had decreased weight average molecular weights, Mw (to 390–600 kDa), and increased values of polydispersity (3.2–4.6) compared to the P(3HB). PHA copolymers showed different thermal behavior: an insignificant decrease in Tmelt and the presence of the second peak in the melting region and changes in parameters of crystallization and glass transition. At the same time, they retained thermostability, and the difference between Tmelt and Tdegr was at least 100–120 °C. Incorporation of 4HB, 3HV, and 3HHx monomer units into the 3-hydroxybutyrate chain caused changes in the amorphous to crystalline ratio and decreased the degree of crystallinity (Cx) to 20–40%. According to the degree to which the monomers reduced crystallinity, they were ranked as follows: 4HB – 3HHx – 3HV. A unique set of films was produced; their surface properties and physical/mechanical properties were studied as dependent on PHA composition; monomers other than 3-hydroxybutyrate were found to enhance hydrophilicity, surface development, and elasticity of polymer films. Conclusion: An innovative set of PHA copolymers was synthesized and solution-cast films were prepared from them; the copolymers and films were investigated as dependent on polymer chemical composition. Results obtained in the present study contribute to the solution of a critical issue of producing degradable polymer materials. © 2021 Elsevier B.V.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M. I.
Заглавие : Oil Spills in Fresh Waters and State of Ecosystem of Lake Pyasino before the Incidental Spill of 2020
Колич.характеристики :10 с
Коллективы : Siberian Branch of the Russian Academy of SciencesRussian Academy of Sciences [223-EP2020/07]; Basic Research of the Russian Federation [51.1.1]
Место публикации : Contemp. Probl. Ecol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2021. - Vol. 14, Is. 4. - С. 313-322. - ISSN 1995-4255, DOI 10.1134/S1995425521040041. - ISSN 1995-4263(eISSN)
Примечания : Cited References:50. - This work was supported by Federal Tasks no. 223-EP2020/07 with the Siberian Branch of the Russian Academy of Sciences and by State Assignment as a part of Basic Research of the Russian Federation, topic no. 51.1.1.
Предметные рубрики: POLYUNSATURATED FATTY-ACIDS
PECHORA BASIN
RIVER
ZOOPLANKTON
Аннотация: This article presents the history of large oil spills in freshwaters, considering the processes of physicochemical and biological degradation of oil. It discusses the toxicity of oil for hydrobionts and effects of oil pollution on communities of plankton, benthos, and ichthyofauna, as well as challenges in mitigating the environmental impact of oil spills. The discussion is concerned with the state of the ecosystem in Lake Pyasino before the incidental spill of 2020, specifically, hydrochemical indicators; species composition; and abundance and biomass of plankton, benthos, and fish. Candidate technologies for restoring the Lake Pyasino ecosystem are reviewed, including "bottom-up" biomanipulation.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Velichko V. V., Tikhomirov A. A., Ushakova S. A., Trifonov S. V., Gribovskaya I. V.
Заглавие : The effect of supplementation of the soil-like substrate with wheat straw mineralized to different degrees on wheat productivity in closed ecosystems
Место публикации : Life Sci. Space Res.: Elsevier Ltd, 2020. - Vol. 26. - С. 132-139. - ISSN 22145524 (ISSN), DOI 10.1016/j.lssr.2020.06.001
Аннотация: Successful incorporation of soil-like substrate (SLS) into biotechnical life support systems is often complicated by the necessity to maintain the balance between flows of mineral elements taken up from the substrate by growing plants and mineral elements added to the SLS as components of mineralized plant inedible biomass. An imbalance between these two flows can be caused by the addition of recalcitrant plant waste such as wheat straw. The purpose of this study was to determine whether the availability of essential nutrients to be taken up by the roots of the wheat plants grown on the SLS could be enhanced by supplementing the SLS with the products derived from wheat straw subjected to different levels of physicochemical mineralization in the aqueous solution of hydrogen peroxide. Different degrees of straw mineralization were achieved by using different ratios of the aqueous solution of hydrogen peroxide to straw. The study showed that supplementation of the SLS with insufficiently oxidized products of physicochemical mineralization of straw resulted in a decrease in the grain yields. The inhibitory effect of the straw subjected to physicochemical oxidation increased with a decrease in the degree to which the straw had been oxidized. Only supplementation with the straw mineralized to the highest possible degree did not inhibit plant growth and development, and the crop yield in that treatment was higher than in the other treatments. © 2020
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mitiouchkina T., Mishin A. S., Somermeyer L. G., Markina N. M., Chepurnyh T. V., Guglya E. B., Karataeva T. A., Palkina K. A., Shakhova E. S., Fakhranurova L. I., Chekova S. V., Tsarkova A. S., Golubev Y. V., Negrebetsky V. V., Dolgushin S. A., Shalaev P. V., Shlykov D., Melnik O. A., Shipunova V. O., Deyev S. M., Bubyrev A. I., Pushin A. S., Choob V. V., Dolgov S. V., Kondrashov F. A., Yampolsky I. V., Sarkisyan K. S.
Заглавие : Author Correction: Plants with genetically encoded autoluminescence (Nature Biotechnology, (2020), 10.1038/s41587-020-0500-9)
Место публикации : Nat. Biotechnol.: Nature Research, 2020. - Article in press. - ISSN 10870156 (ISSN), DOI 10.1038/s41587-020-0578-0
Аннотация: In the version of this article initially published online, a sentence was missing from the Acknowledgements section: The authors would like to acknowledge the work of Genomics Core Facility of the Skolkovo Institute of Science and Technology, which performed the sequencing and bioinformatic analysis. The error has been corrected in the HTML and PDF versions of the article. © 2020, The Author(s), under exclusive licence to Springer Nature America, Inc.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Larionova M. D., Markova S. V., Tikunova N. V., Vysotski E. S.
Заглавие : The smallest isoform of Metridia longa luciferase as a fusion partner for hybrid proteins
Место публикации : Int. J. Mol. Sci.: MDPI AG, 2020. - Vol. 21, Is. 14. - Ст.4971. - С. 1-16. - ISSN 16616596 (ISSN), DOI 10.3390/ijms21144971
Аннотация: Bioluminescent proteins are widely used as reporter molecules in various in vitro and in vivo assays. The smallest isoform of Metridia luciferase (MLuc7) is a highly active, naturally secreted enzyme which, along with other luciferase isoforms, is responsible for the bright bioluminescence of marine copepod Metridia longa. In this study, we report the construction of two variants of a hybrid protein consisting of MLuc7 and 14D5a single-chain antibody to the surface glycoprotein E of tick-borne encephalitis virus as a model fusion partner. We demonstrate that, whereas fusion of a single-chain antibody to either N-or C-terminus of MLuc7 does not affect its bioluminescence properties, the binding site on the single-chain antibody influences its binding capacity. The affinity of 14D5a-MLuc7 hybrid protein (KD = 36.2 nM) where the C-terminus of the single-chain antibody was fused to the N-terminus of MLuc7, appeared to be 2.5-fold higher than that of the reverse, MLuc7-14D5a (KD = 87.6 nM). The detection limit of 14D5a-MLuc7 hybrid protein was estimated to be 45 pg of the recombinant glycoprotein E. Although the smallest isoform of M. longa luciferase was tested as a fusion partner only with a single-chain antibody, it is reasonable to suppose that MLuc7 can also be successfully used as a partner for genetic fusion with other proteins. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Chang D., Liu Y., Chen Y., Hu X., Burov A., Puzyr A., Bondar V., Yao L.
Заглавие : Study of the immunogenicity of the VP2 protein of canine parvovirus produced using an improved Baculovirus expression system
Место публикации : BMC Vet. Res.: BioMed Central Ltd., 2020. - Vol. 16, Is. 1. - Ст.202. - ISSN 17466148 (ISSN), DOI 10.1186/s12917-020-02422-3
Аннотация: Background: Canine parvovirus (CPV) is now recognized as a serious threat to the dog breeding industry worldwide. Currently used CPV vaccines all have their specific drawbacks, prompting a search for alternative safe and effective vaccination strategies such as subunit vaccine. VP2 protein is the major antigen targeted for developing CPV subunit vaccine, however, its production in baculovirus expression system remains challenging due to the insufficient yield. Therefore, our study aims to increase the VP2 protein production by using an improved baculovirus expression system and to evaluate the immunogenicity of the purified VP2 protein in mice. Results: The results showed that high-level expression of the full length VP2 protein was achieved using our modified baculovirus expression system. The recombinant virus carrying two copies of VP2 gene showed the highest expression level, with a productivity of 186 mg/L, which is about 1.4-1.6 fold that of the recombinant viruses carrying only one copy. The purified protein reacted with Mouse anti-His tag monoclonal antibody and Rabbit anti-VP2 polyclonal antibody. BALB/c mice were intramuscularly immunized with purified VP2 protein twice at 2 week intervals. After vaccination, VP2 protein could induce the mice produce high level of hemagglutination inhibition antibodies. Conclusions: Full length CPV VP2 protein was expressed at high level and purified efficiently. Moreover, it stimulated mice to produce high level of antibodies with hemmaglutination inhibition properties. The VP2 protein expressed in this study could be used as a putative economic and efficient subunit vaccine against CPV infection. © 2020 The Author(s).
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tikhomirov A. A., Ushakova S. A., Shikhov V. N., Shklavtsova E. S.
Заглавие : Conceptual approach to selecting radiation spectrum of lamps for plant cultivation in artificial conditions
Место публикации : Light Eng.: LLC Editorial of Journal "Light Technik", 2019. - Vol. 27, Is. Special Issue. - С. 24-39. - ISSN 02362945 (ISSN)
Аннотация: Based on own and literature data, the review of existing approaches to justification of concepts of selecting contemporary light sources for plant cultivation in greenhouse conditions is presented. The work covers comparative experimental data on productivity of lettuce leaves formed under radiation of light-emitting diode emitters with a phytospectrum and white light. A high-pressure sodium lamp was taken as a control source. Lack of valid differences between this data is found out. The article discusses data of literature where effect of heat radiation on plant productivity was discovered by means of light sources of different generations (metal halide lamps, xenon lamps), which is an additional factor apart from visible radiation spectrum which is capable of influencing photobiological efficiency of lamps. On the basis of data of literature, the role of background natural solar light in greenhouses which can negatively affect spectral efficiency of artificial light sources used for supplementary lighting of plants is discussed. A set of methodological factors influencing spectral efficiency of radiation for plant productivity as well as aspects of selection of lamps for cultivation of plants in greenhouse conditions for different latitudes are reviewed and scientifically validated. A controversy in selection of PAR chromaticity of light sources for plant cultivation is demonstrated. The concept of selecting 2 a light source for plant cultivation based on availability of specific response of plant groups to PAR chromaticity is proposed as the most objective for practical application. © 2019, LLC Editorial of Journal "Light Technik". All rights reserved.
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13.

Вид документа : Статья из сборника (выпуск продолж. издания)
Шифр издания :
Автор(ы) : Shevyrnogov A. P., Yu Botvich I., Yemelianov D. V., Larko A. A., Ivchenko V. K., Demianenko T. N.
Заглавие : Estimation of the spatial distribution of spring barley yield using ground-based and satellite spectrophotometric data
Место публикации : IOP Conference Series: Earth and Environmental Science: Institute of Physics Publishing, 2019. - Vol. 315: International Scientific Conference on Agribusiness, Environmental Engineering and Biotechnologies, AGRITECH 2019 (20 June 2019 through 22 June 2019, ) Conference code: 152072, Is. 3. - Ст.032023. - , DOI 10.1088/1755-1315/315/3/032023
Аннотация: The article presents a method for estimating the spatial distribution of spring barley yield, based on the use of optical ground and satellite spectral data (PlanetScope data with a spatial resolution of 3 meters). This approach is highly relevant for the development of precision agriculture technologies. Yield mapping is carried out on the basis of data on the spatial distribution of the actual yield and the spatial distribution of the spectral optical characteristics. The method's characteristic feature is the use of the integral values of vegetation indices (NDVI, MSAVI2, ClGreen) at various stages of crop development. The method was tested on the basis of stationary field experience, where traditional agriculture (deep plowing) is compared with resource-saving technologies (subsurface and surface plowing, and direct seeding with zero tillage). © 2019 IOP Publishing Ltd. All rights reserved.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tikhomirov, Alexander A., Ushakova, Sofya A., Shikhov, Valentin N., Shklavtsova, Ekaterina S.
Заглавие : CONCEPTUAL APPROACH TO SELECTING RADIATION SPECTRUM OF LAMPS FOR PLANT CULTIVATION IN ARTIFICIAL CONDITIONS
Колич.характеристики :7 с
Место публикации : Light Eng.: ZNACK PUBLISHING HOUSE, 2019. - Vol. 27. - С. 24-30. - ISSN 0236-2945
Примечания : Cited References:17
Предметные рубрики: QUALITY
Аннотация: Based on own and literature data, the review of existing approaches to justification of concepts of selecting contemporary light sources for plant cultivation in greenhouse conditions is presented. The work covers comparative experimental data on productivity of lettuce leaves formed under radiation of light-emitting diode emitters with a phytospectrum and white light. A high-pressure sodium lamp was taken as a control source. Lack of valid differences between this data is found out. The article discusses data of literature where effect of heat radiation on plant productivity was discovered by means of light sources of different generations (metal halide lamps, xenon lamps), which is an additional factor apart from visible radiation spectrum which is capable of influencing photobiological efficiency of lamps. On the basis of data of literature, the role of background natural solar light in greenhouses which can negatively affect spectral efficiency of artificial light sources used for supplementary lighting of plants is discussed. A set of methodological factors influencing spectral efficiency of radiation for plant productivity as well as aspects of selection of lamps for cultivation of plants in greenhouse conditions for different latitudes are reviewed and scientifically validated. A controversy in selection of PAR chromaticity of light sources for plant cultivation is demonstrated. The concept of selecting a light source for plant cultivation based on availability of specific response of plant groups to PAR chromaticity is proposed as the most objective for practical application.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kirillova M. A., Ranjan R., Esimbekova E. N., Kratasyuk V. A.
Заглавие : Role of Hsp90 and ATP in modulating apyrase activity and firefly luciferase kinetics
Место публикации : Int. J. Biol. Macromol.: Elsevier B.V., 2019. - Vol. 131. - С. 691-696. - ISSN 01418130 (ISSN) , DOI 10.1016/j.ijbiomac.2019.03.110
Ключевые слова (''Своб.индексиров.''): bioluminescence--heat shock protein 90--high-throughput screening--adenosine triphosphate--apyrase--bovine serum albumin--firefly luciferase--heat shock protein 90--stabilizing agent--article--bioluminescence--clinical study--conformation--controlled study--denaturation--enzyme activity--enzyme kinetics--high throughput screening--incubation time--nonhuman--protein protein interaction--protein refolding--temperature--thermal denaturation--time
Аннотация: The present manuscript describes a novel bioassay consisting of apyrase and heat shock protein 90 (Hsp90) without additional co-chaperone supplementation; intended for high-throughput screening of anti-cancer drugs and prognosis of stress. In this regard, Hsp90 and adenosine 5?-triphosphate (ATP) mediated firefly luciferase (FLuc) kinetics was investigated using apyrase and FLuc as client proteins. Bioluminescent assay containing Hsp90, ATP, and apyrase led to complete loss of luminescence at 50 °C which indicates the protective role of Hsp90 against thermal denaturation. Similarly, the assay sample comprising Hsp90, ATP, and FLuc showed 2 fold increments in luminescence than their counterparts. Introduction of bovine serum albumin (BSA) to the pre-incubated assay mixture led to an initial rise in the luminescence (28%) in comparison to the sample containing Hsp90, ATP and FLuc. Therefore, FLuc based HTS assays are not suitable for clinical samples which may contain stabilizing agents. However, thermally denatured FLuc and apyrase could not regain their active conformation even when Hsp90 and ATP were introduced in the assay system. This observation justifies the role of Hsp90 to be protective rather than a reparation agent when acts without co-chaperones. © 2019
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Trifonov S. V., Morozov Y. A., Kozlova T. A.
Заглавие : Processing of household waste in the BTLSS using the wet combustion method
Место публикации : Life Sci. Space Res.: Elsevier Ltd, 2019. - Vol. 21. - С. 22-24. - ISSN 22145524 (ISSN) , DOI 10.1016/j.lssr.2019.02.003
Аннотация: The present study discusses physicochemical methods of organic waste processing in closed biotechnical life support systems (BTLSS). Sanitary and household cotton wastes were processed by the method of wet combustion in hydrogen peroxide using an alternating current electric field – a promising physicochemical method for organic waste processing in the BTLSS. The highest efficiency of the process (in terms of power consumption, duration of the process, and oxidation rate) was achieved in experiments with oxidation of a combination of cotton fabrics and urea-containing wastes such as human urine and feces. The reason for this must be that urea is a reactive aqueous solvent of cellulose. © 2019
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shevyrnogov A. P., Pisman T. I., Kononova N. A., Botvich, I. Yu., Larko A. A., Vysotskaya G. S.
Заглавие : Seasonal Dynamics of Vegetation on Fallow Lands in Krasnoyarsk Forest Steppe According to Terrain and Satellite Data
Колич.характеристики :9 с
Коллективы : Complex Program of Fundamental Research of the Siberian Branch of the Russian Academy of Sciences [74, AAAA-A17-117013050027-1]
Место публикации : Izv. Atmos. Ocean. Phys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2019. - Vol. 55, Is. 9. - С. 1353-1361. - ISSN 0001-4338, DOI 10.1134/S0001433819090470. - ISSN 1555-628X(eISSN)
Примечания : Cited References:29. - This study was performed according to the Complex Program of Fundamental Research of the Siberian Branch of the Russian Academy of Sciences Interdisciplinary Integration Researches for 2018-2020 (project no. 74) and State Task registration no. AAAA-A17-117013050027-1.
Предметные рубрики: MODIS
Аннотация: This article presents investigation data on the seasonal dynamics of productivity, status, and species composition of vegetation on fallow lands in the Krasnoyarsk forest steppe (Middle Siberia) obtained from terrain and satellite materials from 2017. The results of the study of grass plant communities on the basis of geobotanical descriptions and terrain spectrometry were have been used for a more accurate interpretation of cosmic photographs of moderate and low resolution. For studying vegetation on fallow lands, we analyze the seasonal dynamics of various vegetative indices (NDVI, EVI, LSWI, and LAI) and parameters (NPP, FPAR, and LST (land surface temperature)) obtained from MODIS satellite images. Our analysis of satellite data shows the absence of evidences of plowing and mowing in the studied area. A positive correlation is revealed between vegetation indices of biomass (NDVI, EVI, LAI, and NPP) and parameters of hydrothermal conditions (LSWI, FPAR, and LST).
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Makhrov A. A., Artamonova V. S., Kolmakova O. V., Ponomareva M. V.
Заглавие : Sex Determination Model in Pink Salmon Oncorhynchus gorbuscha (Walbaum, 1792) (Salmonidae, Osteichthyes) Controlled by Multi-Copy Genes Located in Sex Chromosomes
Колич.характеристики :4 с
Коллективы : Russian Science Foundation [16-14-10001]
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2018. - Vol. 478, Is. 1. - С. 21-24. - ISSN 1607-6729, DOI 10.1134/S1607672918010064. - ISSN 1608-3091(eISSN)
Примечания : Cited References:14. - This work was supported by the Russian Science Foundation (project no. 16-14-10001).
Предметные рубрики: FISH
Аннотация: This article is devoted to presenting the hypothesis explaining the fact of a considerable prevalence of phenotypic males among the triploid pink salmon as well as the regular occurrence of intersexes, which were revealed by us. This hypothesis also explains the large proportion (in some cases) in pink salmon populations of the individuals whose genetic sex does not match the phenotypic sex. We assume that the genes encoding the factors that contribute to the transformation of individuals into males (but not the marker sequences of the Y chromosome) are present not only in the Y chromosome of pink salmon but also in the X chromosome, although in smaller quantities.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Markova, Svetlana V., Larionova, Marina D., Vysotski, Eugene S.
Заглавие : Shining Light on the Secreted Luciferases of Marine Copepods: Current Knowledge and Applications.
Место публикации : Photochemistry and photobiology. - 2018. - ISSN 1751-1097, DOI 10.1111/php.13077
Аннотация: Copepod luciferases - a family of small secretory proteins of 18.4-24.3 kDa, including a signal peptide, are responsible for bright secreted bioluminescence of some marine copepods. The copepod luciferases use coelenterazine as a substrate to produce blue light in a simple oxidation reaction without any additional cofactors. They do not share sequence or structural similarity with other identified bioluminescent proteins including coelenterazine-dependent Renilla and Oplophorus luciferases. The small size, strong luminescence activity and high stability, including thermostability, make secreted copepod luciferases very attractive candidates as reporter proteins which are particularly useful for nondisruptive reporter assays and for high-throughput format. The most known and extensively investigated representatives of this family are the first cloned GpLuc and MLuc luciferases from copepods Gaussia princeps and Metridia longa, respectively. Immediately after cloning these homologous luciferases were successfully applied as bioluminescent reporters in vivo and in vitro, and since then the scope of their applications continues to grow. This review is an attempt to systemize and critically evaluate the data scattered through numerous articles regarding the main structural features of copepod luciferases, their luminescent and physicochemical properties. We also review the main trends of their application as bioluminescent reporters in cell and molecular biology. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Velichko V. V., Tikhomirov A. A., Ushakova S. A.
Заглавие : Estimating CO2 gas exchange in mixed age vegetable plant communities grown on soil-like substrates for life support systems
Место публикации : Life Sci. Space Res.: Elsevier Ltd, 2018. - Vol. 16. - С. 47-51. - ISSN 22145524 (ISSN) , DOI 10.1016/j.lssr.2017.11.001
Ключевые слова (''Своб.индексиров.''): bioconversion of plant waste--co2 gas exchange--conveyor mode--plant cultivation--soil-like substrate--carbon dioxide--article--atmosphere--beet--carrot--concentration (parameters)--cyperus esculentus--gas exchange--genetic variation--microclimate--nonhuman--plant age--plant community--plant growth--planting density--priority journal--reproducibility--soil and soil related phenomena--soil like substrate--vegetable
Аннотация: If soil-like substrate (SLS) is to be used in human life support systems with a high degree of mass closure, the rate of its gas exchange as a compartment for mineralization of plant biomass should be understood. The purpose of this study was to compare variations in CO2 gas exchange of vegetable plant communities grown on the soil-like substrate using a number of plant age groups, which determined the so-called conveyor interval. Two experimental plant communities were grown as plant conveyors with different conveyor intervals. The first plant community consisted of conveyors with intervals of 7 days for carrot and beet and 14 days for chufa sedge. The conveyor intervals in the second plant community were 14 days for carrot and beet and 28 days for chufa sedge. This study showed that increasing the number of age groups in the conveyor and, thus, increasing the frequency of adding plant waste to the SLS, decreased the range of variations in CO2 concentration in the “plant–soil-like substrate” system. However, the resultant CO2 gas exchange was shifted towards CO2 release to the atmosphere of the plant community with short conveyor intervals. The duration of the conveyor interval did not significantly affect productivity and mineral composition of plants grown on the SLS. © 2017
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