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Changes in athletes’ psycho-emotional stress in the course of annual macrocycle / M. A. Gamzin [et al.] // J. Siberian Fed. Univ. Humanit. Social Sci. - 2019. - Vol. 12, Is. 6. - P988-995, DOI 10.17516/1997–1370–0436 . - ISSN 1997-1370
Кл.слова (ненормированные):
Athletes -- Electroencephalography -- Psycho-emotional stress
Аннотация: Emotions significantly influence physiological processes in the athlete’s body. The level of his/ her mental stress during training, competitions and recreation is obviously not the same. Taking into account the athletes’ well-known predisposition to alexithymia, there is a need to search for objective ways of the level of psycho-emotional stress assessment. Using the calculated Stress Index (SI), based on electroencephalogram indicators and level of situational anxiety as per the standard Spielberger-Hanin test, our work assesses and compares the differences in the athletes’ psycho-emotional state in various phases of the annual macrocycle. The study involved 155 athletes doing various sports. 96 athletes were males, their average age being 24.34 ± 3.54 years; 59 athletes were females with the average age of 23.12 ± 2.3 years. The control group consisted of 101 individuals who did not experience systematic high physical exertion, 53 of them being males with the average age of 23.17 ± 2.54, 48 — females with the average age of 22.12 ± 3.01 years. The athletes’ SI values are significantly different from those in the control group, as well as among themselves at various stages of the annual macrocycle. In the preparatory and transitional periods, the SI is significantly lower than in the control group. However, during the competitive period this index increases considerably. This is characteristic of a significant increase in the athletes’ psycho-emotional stress. There is a high positive correlation (r = 0.81) between the SI value and the number of points scored in the Spielberger-Hanin situational anxiety test. © Siberian Federal University. All rights reserved.

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Держатели документа:
Academy Fight behalf D.G. Mindiashvili, 88 Markovskogo Str., Krasnoyarsk, 660049, Russian Federation
Institute of Biophysics SB RAS, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Research Institute for Medical Problems, North, 3g Partizana Zheleznyaka Str, Krasnoyarsk, 660022, Russian Federation
Siberian Federal University, 79 Svobodny, Krasnoyarsk, 660041, Russian Federation
Reshetnev Siberian State University of Science and Technology, 31 Krasnoyarsky Rabochy, Krasnoyarsk, 660037, Russian Federation

Доп.точки доступа:
Gamzin, M. A.; Bazarin, K. P.; Savchenko, A. A.; Kakukhin, A. D.

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Benefits of biophysical tests of saliva for athletic functionality rating tests / A. M. Vyshedko [и др.] // Teor. Prakt. Fiz. Kult. - 2019. - Vol. 2019, Is. 7. - С. 65-67 . - ISSN 0040-3601
Кл.слова (ненормированные):
Antioxidant activity rate -- Bioluminescence test -- Chemoluminescence test -- Elite sports -- Residual luminescence -- Saliva
Аннотация: Modern physical training systems give a high priority to the training process individualizing elements that may be efficient enough only when supported by objective tests to rate the bodily responses to physical workloads. Moreover, success in modern professional sports is impossible unless supported by the newest medical/ biological findings and technologies in the health rating and forecast domains, with a special role played by the modern biophysical research of the cellular- and molecular-level biological processes. The study was designed to assess benefits of biophysical analysis of saliva for the athletic functionality rating tests. Sampled for the purposes of the study were athletes of different skill levels (from Class I to Honorary Masters of Sport) versus the untrained students, with the saliva sampled prior to and after physical trainings by bioluminescence and chemoluminescence test methods. The test data were processed by Statistica 10 (made by StatSoft Inc., the US) toolkit to produce median values (Me) and inter-quartile distribution (C25-C75 percentile) rates. The Mann-Whitney nonparametric U-test of the null hypothesis was used to rate differences of the independent variables, with the statistical difference rated meaningful at p<0.05. The bioluminescence tests found the higher ferment activity of saliva in the athletes’ versus untrained students, with the ferment activity in the bioluminescence tests also tested to grow after physical workloads in the highly-skilled athletes and fall in the untrained students. The study found the saliva antioxidant system functional intensity being athletic skills dependent. Therefore, the study has demonstrated benefits of the biophysical (bioluminescence and chemoluminescence) tests of saliva for the athletic functionality rating tests in the physical workload design and management initiatives. © 2019, Teoriya i praktika fizicheskoy kul'tury i sporta. All rights reserved.

Scopus
Держатели документа:
Siberian Federal University, Krasnoyarsk, Russian Federation
Research Institute of Medical Problems of the North, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Vyshedko, A. M.; Stepanova, L. V.; Kolenchukova, O. A.; Kratasyuk, V. A.

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A noninvasive and qualitative bioluminescent assay for express diagnostics of athletes' responses to physical exertion / V. A. Kratasyuk, L. V. Stepanova, R. Ranjan [et al.] // Luminescence. - 2020, DOI 10.1002/bio.3954. - Cited References:33. - The Ministry of Science and Higher Education of the Russian Federation, Grant/Award Number: FSRZ-2020-0006; Krasnoyarsk Regional Foundation of Science, Grant/Award Number: KF-537 . - Article in press. - ISSN 1522-7235. - ISSN 1522-7243

РУБ Chemistry, Analytical
Рубрики:
SALIVARY BIOMARKERS
EXERCISE
Кл.слова (ненормированные):
athletes -- BLuc‐ -- Red coupled enzyme system -- catalase activity -- saliva -- training load
Аннотация: Upcoming professional sports authorities seek rapid noninvasive biosensing tools for regular monitoring of athletes' physiological states. The analysis of saliva through luminescence-based biosensors has been perceived as a suitable candidate for such purposes. The present study reports a qualitative bioluminescence assay based on a coupled enzyme system that consists of bacterial luciferase (BLuc) and nicotinamide adenine dinucleotide (NADH):flavin mononucleotide (FMN) oxidoreductase (Red), BLuc-Red, for the express diagnostics of athletes' stress levels before and after physical exertion. The volunteers who participated in the study were grouped as freestyle wrestlers and students who adapted to different levels of physical activities. Under physical exertion modelling conditions, the influence of participant saliva on BLuc-Red catalyzed light emission was investigated. Results showed a significant increase in residual luminescence (I-exp, mean maximum bioluminescence intensity of the experimental measurement (I-exp); I-c, luminescence intensity in control; I-exp/I-c, %) values for participants in the wrestler group while a decrease in the student group (P < 0.05). Such contrasting residual luminescence values in both groups were found to be dependent on the catalase activity of saliva. The proposed bioluminescence assay can be utilized as a potential nonspecific biosensing tool for determining the physical state of athletes under high loads.

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Держатели документа:
Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Dept Biophys, Svobodny Prospect 79, Krasnoyarsk 660041, Russia.
Inst Biophys SB RAS, Fed Res Ctr Krasnoyarsk Sci Ctr SB RAS, Akademgorodok 50-50, Akademgorodok, Russia.
Krasnoyarsk State Med Univ, Minist Hlth Russian Federat, Av Partizan Zheleznyak 1, Krasnoyarsk, Russia.
Krasnoyarsk Matern & Childhood Protect Ctr, Kirenskogo St 2a, Krasnoyarsk, Russia.
Siberian Fed Univ, Sch Nonferrous Met & Mat Sci, Svobodny Prospect 79, Krasnoyarsk, Russia.
Sci Res Inst Med Problems North, Av Partizan Zheleznyak 3g, Krasnoyarsk, Russia.

Доп.точки доступа:
Kratasyuk, Valentina A.; Stepanova, Lyudmila, V; Ranjan, Rajeev; Sutormin, Oleg S.; Pande, Shubhra; Zhukova, Galina, V; Miller, Olga M.; Maznyak, Natalya, V; Kolenchukova, Oksana A.; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]; Krasnoyarsk Regional Foundation of Science [KF-537]

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