Библиотека института биофизики СО РАН

Главная

Базы данных

Труды сотрудников ИБФ СО РАН - результаты поиска

Вид поиска

Каталог книг и продолжающихся изданий библиотеки Института биофизики СО РАН

Иностранные журналы библиотеки Института биофизики СО РАН

Отечественные журналы библиотеки Института биофизики СО РАН

Каталог диссертаций ИБФ СО РАН

Труды сотрудников ИБФ СО РАН

Область поиска

в найденном

Формат представления найденных документов:
полный	информационный	краткий

Отсортировать найденные документы по:
автору	заглавию	году издания	типу документа

Поисковый запрос: (<.>K=bacterium<.>)

Общее количество найденных документов : 106
Показаны документы с 1 по 20

1-20 21-40 41-60 61-80 81-100 101-106

^a314.27.17^2VINITI
Ч-25

Частичная реконструкция in vitro люминесцентной системы светящихся бактерий [Текст] : научное издание / В. В. Межевикин [и др.] // Микробиология. - 1985. - Т. 54, N 2. - С. 209-213 . - ISSN 0026-3656

ГРНТИ

31.27.17

РУБ 314.27.17
Рубрики:
PHOTOBACTERIUM LEIOGNATHI
   БАКТЕРИИ
   СВЕТЯЩИЕСЯ
   МЕТАБОЛИЧЕСКАЯ ОРГАНИЗАЦИЯ
   ЛЮМИНЕСЦЕНТНАЯ СИСТЕМА
   LUMINESCENT BACTERIUM SYSTEM
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Межевикин, В.В.; Высоцкий, Е.С.; Заворуев, В.В.; Кузнецов, П.В.; Райбекас, А.А.

Найти похожие

Vertical stratification of physical, chemical and biological components in two saline lakes Shira and Shunet (South Siberia, Russia) / A. G. Degermendzhy [et al.] // Aquatic Ecology. - 2010. - Vol. 44, Is. 3. - P619-632, DOI 10.1007/s10452-010-9336-6 . - ISSN 1386-2588
Кл.слова (ненормированные):
Ciliates -- Cryptomonas -- Fishless lakes -- Gammarus -- Mathematical modelling -- Meromictic lakes -- Phytoplankton -- Stratification -- Sulphur bacteria -- amphipod -- bacterium -- biomass -- ciliate -- ecosystem modeling -- flagellate -- meromictic lake -- microbial community -- numerical model -- physicochemical property -- phytoplankton -- population density -- saline lake -- salinity -- stratification -- thermocline -- trophic interaction -- vertical profile -- zooplankton -- Khakassia -- Lake Shira -- Lake Shunet -- Russian Federation -- Siberia -- Amphipoda -- Bacteria (microorganisms) -- Ciliophora -- Copepoda -- Cryptomonas -- Cryptomonas sp. -- Gammaridae -- Gammarus -- Gammarus lacustris -- Phytomastigophorea -- Protista -- Rotifera
Аннотация: A feature of meromictic lakes is that several physicochemical and biological gradients affect the vertical distribution of different organisms. The vertical stratification of physical, chemical and biological components in saline, fishless meromictic lakes Shira and Shunet (Siberia, Russia) is quite different mainly because both mean depth and maximum depth of lakes differ as well as their salinity levels differ. The chemocline of the Lake Shira, as in many meromictic lakes, is inhabited by bacterial community consisting of purple sulphur and heterotrophic bacteria. As the depth of the chemocline is variable, the bacterial community does not attain high densities. The mixolimnion in Lake Shira, which is thermally stratified in summer, also creates different habitat for various species. The distribution of phytoplankton is non-uniform with its biomass peak in the metalimnion. The distribution of zooplankton is also heterogeneous with rotifers and juvenile copepods inhabiting the warmer epilimnion and older copepods found in the cold but oxic hypolimnion. The amphipod Gammarus lacustris which can be assigned to the higher trophic link in the fishless lake's ecosystem, such as Lake Shira, is also distributed non-uniformly, with its peak density generally observed in the thermocline region. The chemocline in Lake Shunet is located at the depth of 5 m, and unlike in Lake Shira, due to a sharp salinity gradient between the mixolimnion and monimolimnion, this depth is very stable. The mixolimnion in Lake Shunet is relatively shallow and the chemocline is inhabited by (1) an extremely dense bacterial community; (2) a population of Cryptomonas sp.; and (3) ciliate community comprising several species. As the mixolimnion of Lake Shunet is not thermally stratified for long period, the phytoplankton and zooplankton populations are not vertically stratified. The gammarids, however, tend to concentrate in a narrow layer located 1-2 m above the chemocline. We believe that in addition to vertical inhomogeneities of both physicochemical parameters, biological and physical factors also play a role in maintaining these inhomogeneities. We conclude that the stratified distributions of the major food web components will have several implications for ecosystem structure and dynamics. Trophic interactions as well as mass and energy flows can be significantly impacted by such heterogeneous distributions. Species spatially separated even by relatively short distances, say a few centimetres will not directly compete. Importantly, we demonstrate that not only bacteria, phytoflagellates and ciliate tend to concentrate in thin layers but also larger-sized species such Gammarus (amphipods) can also under certain environmental conditions have stratified distribution with maxima in relatively thin layer. As the vertical structure of the lake ecosystem is rather complex in such stratified lakes as ours, the strategy of research, including sampling techniques, should consider potentially variable and non-homogeneous distributions. В© 2010 The Author(s).

Scopus
Держатели документа:
Institute of Biophysics SB RAS, 660036 Krasnoyarsk, Akademgorodok, Russian Federation
Siberian Federal University, Svobodnyi 79, 660041 Krasnoyarsk, Russian Federation
Netherlands Environmental Assessment Agency (PBL), P.O. Box 303, 3720 AH Bilthoven, Netherlands
Department of Aquatic Ecology, Netherlands Institute of Ecology (NIOO-KNAW), 3631 AC Nieuwersluis, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Degermendzhy, A.G.; Zadereev, E.S.; Rogozin, D.Y.; Prokopkin, I.G.; Barkhatov, Y.V.; Tolomeev, A.P.; Khromechek, E.B.; Janse, J.H.; Mooij, W.M.; Gulati, R.D.

Найти похожие

Trends in microevolution of microbial populations in open systems / A. V. Brilkov [et al.] // Doklady Biochemistry and Biophysics. - 2005. - Vol. 404, Is. 1-6. - P349-352, DOI 10.1007/s10628-005-0111-x . - ISSN 1607-6729
Кл.слова (ненормированные):
article -- bacterial phenomena and functions -- bacterium -- biological model -- culture technique -- Escherichia coli -- evolution -- genetics -- growth, development and aging -- mathematics -- methodology -- mutation -- nanotechnology -- pH -- physiology -- population dynamics -- time -- Bacteria -- Bacterial Physiology -- Cell Culture Techniques -- Escherichia coli -- Evolution -- Hydrogen-Ion Concentration -- Mathematics -- Models, Biological -- Mutation -- Nanotechnology -- Population Dynamics -- Time Factors

Scopus
Держатели документа:
Krasnoyarsk State University, Krasnoyarsk, 660062, Russian Federation
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Brilkov, A.V.; Loginov, I.A.; Morozova, E.V.; Pechurkin, N.S.

Найти похожие

Theoretical and experimental decisions in the creation of an artificial ecosystem for human life support in space. / L. V. Kirensky [et al.] // Life sciences and space research. - 1971. - Vol. 9. - P75-80 . - ISSN 0075-9422
Кл.слова (ненормированные):
article -- bacterium -- biotechnology -- Chlorella -- energy metabolism -- human -- instrumentation -- man machine interaction -- metabolism -- microclimate -- space flight -- weightlessness -- Bacteria -- Biotechnology -- Chlorella -- Ecological Systems, Closed -- Energy Metabolism -- Environment, Controlled -- Humans -- Life Support Systems -- Man-Machine Systems -- Space Flight -- Weightlessness
Аннотация: All of man's former space flights were not real ventures into space in the biological sense, as his life was supported with unregenerated earth supplies. The coming stage of space exploration requires man's long existence in the cosmos and on the other planets. This stage of man's activity outside the earth become possible only by creating small man-made ecosystems, permitting the support of his metabolism by the recycling of substances of the terrestrial biosphere. Creation of such systems is a new scientific and technical task. Man-made ecosystems are a new product of man's activity, which have no complete analogy, either in nature, or in technology. Stochastic mechanisms, which stabilize biogeocenosis, cannot be effective in small ecosystems. A technique of parametric control over biosynthesis made it possible to calculate, and put to practice, an ecosystem for man with a cyclic regeneration of the atmosphere, water and, partially, food. The specific bio-technological properties of small man-made ecosystems are being analysed. The possibility of their application for man's excursions into space and for the settlement of other planets is being considered.

Scopus
Держатели документа:
Institute of Physics, Akademgorodok, Krasnoyarsk, USSR. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kirensky, L.V.; Gitelson, I.I.; Terskov, I.A.; Kovrov, B.G.; Lisovsky, G.M.; Okladnikov, Y.N.

Найти похожие

The yellow bioluminescence bacterium, Vibrio fischeri Y1, contains a bioluminescence active riboflavin protein in addition to the yellow fluorescence FMN protein / V. N. Petushkov, B. G. Gibson, J. Lee // Biochemical and Biophysical Research Communications. - 1995. - Vol. 211, Is. 3. - P774-779, DOI 10.1006/bbrc.1995.1880 . - ISSN 0006-291X
Кл.слова (ненормированные):
riboflavin -- article -- bioluminescence -- fluorescence -- nonhuman -- priority journal -- protein analysis -- protein synthesis -- vibrio -- vibrionaceae -- Bacterial Proteins -- Chromatography, Gel -- Chromatography, Thin Layer -- Flavin Mononucleotide -- Flavoproteins -- Luminescence -- Riboflavin -- Spectrometry, Fluorescence -- Support, U.S. Gov't, P.H.S. -- Vibrio -- Bacteria (microorganisms) -- Photobacterium -- Vibrio -- Vibrio fischeri
Аннотация: The yellow bioluminescence Y1 strain of Vibrio fischeri can produce a 22 kDa protein with either FMN or riboflavin as a bound fluorophore. Both forms are active for shifting the bioluminescence spectral maximum. The fluorescence spectral distribution of the two proteins differs slightly and the in vivo emission appears to be an equal mixture of the two. The bioluminescence activity of the riboflavin Y1 protein contrasts with the inactivity of the related Photobacterium type.

Scopus
Держатели документа:
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, United States
Institute of Biophysics, Academy of Sciences of Russia (Siberian Branch), 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Petushkov, V.N.; Gibson, B.G.; Lee, J.

Найти похожие

The use of bioluminescent biotests for study of natural and laboratory aquatic ecosystems / V. A. Kratasyuk [et al.] // Chemosphere. - 2001. - Vol. 42, Is. 8. - P909-915, DOI 10.1016/S0045-6535(00)00177-6 . - ISSN 0045-6535
Кл.слова (ненормированные):
Alcohol dehydrogenase -- Bacterial luciferase -- Bioluminescence -- Blooming -- Pollution -- Trypsin -- Water toxicity -- alcohol dehydrogenase -- benzoquinone -- luciferase -- trypsin -- aquatic ecosystem -- bioluminescence -- water quality -- article -- bacterium culture -- bioluminescence -- blue green alga -- ecosystem -- pond -- seasonal variation -- water pollution -- water quality -- Benzoquinones -- Biological Assay -- Cyanobacteria -- Ecosystem -- Environmental Monitoring -- Eutrophication -- FMN Reductase -- Indicators and Reagents -- Luminescent Measurements -- NADH, NADPH Oxidoreductases -- Water Pollutants -- Russian Federation -- algae -- Bacteria (microorganisms) -- Chlorophyta -- Cyanobacteria -- uncultured cyanobacterium
Аннотация: A set of bioluminescent tests was developed to monitor water quality in natural and laboratory ecosystems. It consisted of four bioluminescent systems: luminous bacteria, coupled enzyme system NADH:FMN-oxidoreductase-luciferase and triplet enzyme systems with alcohol dehydrogenase and trypsin. The set of biotests was applied for a small forest pond (Siberia, Russia), laboratory microecosystems polluted with benzoquinone and a batch culture of blue-green algae. Thereby effects of natural water compared to those of models of heavy pollution and "bloom" of blue-greens on the bioluminescent tests were revealed. The set of biotests was not affected by a natural seasonal variability of water quality in the unpolluted pond, but responded to the heavy pollution and the "bloom" of blue-greens. The set of biotests could be recommended as the alarm test to control the acute toxicity of natural water bodies. В© 2001 Elsevier Science Ltd.

Scopus
Держатели документа:
Krasnoyarsk State University, pr. Svobodnii 79, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Krasnoyarsk State Agricultural University, Mira av., 88, Krasnoyarsk, 660049, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kratasyuk, V.A.; Esimbekova, E.N.; Gladyshev, M.I.; Khromichek, E.B.; Kuznetsov, A.M.; Ivanova, E.A.

Найти похожие

The synthesis of hydroxybutyrate and hydroxyvalerate copolymers by the bacterium Ralstonia eutropha / T. G. Volova, G. S. Kalacheva // Mikrobiologiya. - 2005. - Vol. 74, Is. 1. - С. 63-69 . - ISSN 0026-3656
Кл.слова (ненормированные):
?-ketothiolase -- Controlled synthesis -- Poly(hydroxybutyrate-co-hydroxyvalerate) -- Ralstonia eutropha -- Bacteria (microorganisms) -- Cupriavidus necator -- acetoacetyl coenzyme a -- acetyl coenzyme A acyltransferase -- acyl coenzyme A -- acyltransferase -- butyric acid derivative -- carbon dioxide -- fructose -- hydrogen -- poly(3 hydroxybutyrate) co (3 hydroxyvalerate) -- poly(3-hydroxyalkanoic acid) synthase -- poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate) -- polyester -- polyhydroxyalkanoate synthase -- valeric acid derivative -- article -- chemistry -- crystallization -- culture medium -- metabolism -- Wautersia eutropha -- Acetyl-CoA C-Acyltransferase -- Acyl Coenzyme A -- Acyltransferases -- Butyrates -- Carbon Dioxide -- Crystallization -- Culture Media -- Cupriavidus necator -- Fructose -- Hydrogen -- Polyesters -- Valerates
Аннотация: The paper deals with the study of the synthesis of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) copolymers by the bacterium Ralstonia eutropha B-5786 grown under different carbon nutrition conditions (growth on carbon dioxide, fructose, and CO2-valerate and fructose-valerate mixtures). The parameters to be analyzed included the yield of biomass, the yield, synthesis rate, and composition of copolymers, the activity of the key enzymes of polyhydroxyalkanoate (PHA) synthesis (?-ketothiolase, acetoacetyl-CoA reductase, and PHA synthase), the maximum tolerable concentration of valerate to the bacterium, and the conditions that govern the incorporation of hydroxyvalerate to copolymers. This allowed the relationship between cultivation conditions and the proportion of monomers in the copolymers to be deduced. We were able to synthesize a range of 3HB/3HV copolymers and found that the thermal characteristics and the degree of crystallinity of these copolymers depend on the molar fraction of 3HV.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.

Найти похожие

The synthesis of hydroxybutyrate and hydroxyvalerate copolymers by the bacterium Ralstonia eutropha / T. G. Volova, G. S. Kalacheva // Microbiology. - 2005. - Vol. 74, Is. 1. - P54-59, DOI 10.1007/s11021-005-0028-5 . - ISSN 0026-2617
Кл.слова (ненормированные):
?-ketothiolase -- Controlled synthesis -- Poly(hydroxybutyrate-co-hydroxyvalerate) -- Ralstonia eutropha -- Bacteria (microorganisms) -- Ralstonia -- Wautersia eutropha
Аннотация: The paper deals with the study of the synthesis of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) copolymers by the bacterium Ralstonia eutropha B-5786 grown under different carbon nutrition conditions (growth on carbon dioxide, fructose, and CO2-valerate and fructose-valerate mixtures). The parameters to be analyzed included the yield of biomass; the yield, synthesis rate, and composition of copolymers; the activity of the key enzymes of polyhydroxyalkanoate (PHA) synthesis ?-ketothiolase, acetoacetyl-CoA reductase, and PHA synthase); the maximum tolerable concentration of valerate to the bacterium; and the conditions that govern the incorporation of hydroxyvalerate into copolymers. This allowed the relationship between cultivation conditions and the proportion of monomers in the copolymers to be deduced. We were able to synthesize a range of 3HB/3HV copolymers and found that the thermal characteristics and the degree of crystallinity of such copolymers depend on the molar fraction of 3HV. В© 2005 Pleiades Publishing, Inc.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.

Найти похожие

The "quantization" of sensitivity coefficients is preserved in microbial populations heterogeneous with respect to growth rate and age / V. M. Nekrasov, A. V. Chernychev, A. G. Degermendzhy // Doklady Biological Sciences. - 2006. - Vol. 406, Is. 1-6. - P91-93, DOI 10.1134/S0012496606010261 . - ISSN 0012-4966
Кл.слова (ненормированные):
algorithm -- article -- bacterium -- biodiversity -- growth, development and aging -- population dynamics -- theoretical model -- time -- Algorithms -- Bacteria -- Biodiversity -- Models, Theoretical -- Population Dynamics -- Time Factors

Scopus
Держатели документа:
Institute of Chemical Kinetics and Combustion, Siberian Division, Russian Academy of Sciences, Institutskaya ul. 3, Novosibirsk, 630090, Russian Federation
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Nekrasov, V.M.; Chernychev, A.V.; Degermendzhy, A.G.

Найти похожие

10.

The information value of fatty acid composition of triacylglycerols and polar lipids of seston in assaying the food spectrum of microzooplankton in the bugach reservoir. / O. N. Makhutova, N. N. Sushchik, G. S. Kalacheva // Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections / translated from Russian. - 2004. - Vol. 395. - P143-145 . - ISSN 0012-4966
Кл.слова (ненормированные):
fatty acid -- fresh water -- lipid -- triacylglycerol -- article -- bacterium -- biomass -- chemistry -- Cyanobacterium -- diatom -- ecosystem -- metabolism -- physiology -- phytoplankton -- Russian Federation -- season -- Bacteria -- Biomass -- Cyanobacteria -- Diatoms -- Ecosystem -- Fatty Acids -- Fresh Water -- Lipids -- Phytoplankton -- Russia -- Seasons -- Triglycerides

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Makhutova, O.N.; Sushchik, N.N.; Kalacheva, G.S.

Найти похожие

11.

The effect of succinic acid sulfoderivatives on bacterial luminescence / V. A. Kratasyuk [и др.] // Prikladnaya Biokhimiya i Mikrobiologiya. - 1991. - Vol. 27, Is. 1. - С. 127-133 . - ISSN 0555-1099
Кл.слова (ненормированные):
luciferase -- n (arylsulfonamido)succinimide -- unclassified drug -- article -- bacterium -- bioluminescence -- nonhuman -- Bacteria (microorganisms)

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of the USSR Academy of Sciences, Krasnoyarsk, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kratasyuk, V.A.; Makurina, V.I.; Kuznetsov, A.M.; Kudryasheva, N.S.; Plotnikova, N.B.; Medvedeva, S.E.; Gritsenko, I.S.; Chernykh, V.P.

Найти похожие

12.

The autotrophic synthesis of polyhydroxyalkanoate by alcaligenes eutrophus in the presence of carbon monoxide / T. G. Volova, G. S. Kalacheva, O. V. Altukhova // Mikrobiologiya. - 2001. - Vol. 70, Is. 6. - С. 745-752 . - ISSN 0026-3656
Кл.слова (ненормированные):
CO inhibition -- Hydrogen-oxidizing bacteria -- Polyhydroxyalkanoates (PHAs) -- Synthesis -- alkane -- carbon monoxide -- fatty acid -- hydroxyacid -- lipid -- nitrogen -- polyester -- article -- chemistry -- culture medium -- drug antagonism -- growth, development and aging -- mass spectrometry -- metabolism -- Wautersia eutropha -- Alkanes -- Carbon Monoxide -- Culture Media -- Cupriavidus necator -- Fatty Acids -- Hydroxy Acids -- Lipids -- Mass Spectrometry -- Nitrogen -- Polyesters
Аннотация: The CO-resistant strain B5786 of the hydrogen-oxidizing bacterium Alcaligenes eutrophus was found to be able to synthesize polyhydroxyalkanoates (PHAs) under the conditions of growth limitation by nitrogen deficiency (the factor that promotes PHA synthesis) and growth inhibition by carbon monoxide. The gas mixtures that contained from 5 to 20 vol % CO did not inhibit the key enzymes of PHA synthesis-?-ketothiolase, acetoacetyl-CoA reductase, hydroxybutyrate dehydrogenase, and PHA synthase. In the presence of CO, cells accumulated up to 70-75 wt % PHA (with respect to the dry biomass) without any noticeable increase in the consumption of the gas substrate. Chromatographic-mass spectrometric analysis showed that the PHA synthesized by A. eutrophus is a copolymer containing more than 99 mol % ?-hydroxybutyrate and trace amounts of ?-hydroxyvalerate. The PHA synthesized under the conditions described did not differ from that synthesized by A. eutrophus cells from electrolytic hydrogen.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Altukhova, O.V.

Найти похожие

13.

The autotrophic synthesis of polyhydroxyalkanoate by Alcaligenes eutrophus in the presence of carbon monoxide / T. G. Volova, G. S. Kalacheva, O. V. Altukhova // Microbiology. - 2001. - Vol. 70, Is. 6. - P640-646 . - ISSN 0026-2617
Кл.слова (ненормированные):
CO inhibition -- Hydrogen-oxidizing bacteria -- Polyhydroxyalkanoates (PHAs) -- Synthesis
Аннотация: The CO-resistant strain B5786 of the hydrogen-oxidizing bacterium Alcaligenes eutrophus was found to be able to synthesize polyhydroxyalkanoates (PHAs) under the conditions of growth limitation by nitrogen deficiency (the factor that promotes PHA synthesis) and growth inhibition by carbon monoxide. The gas mixtures that contained from 5 to 20 vol % CO did not inhibit the key enzymes of PHA synthesis-?-ketothiolase, acetoacetyl-CoA reductase, hydroxybutyrate dehydrogenase, and PHA synthase. In the presence of CO, cells accumulated up to 70-75 wt % PHA (with respect to the dry biomass) without any noticeable increase in the consumption of the gas substrate. Chromatographic-mass spectrometric analysis showed that the PHA synthesized by A. eutrophus is a copolymer containing more than 99 mol % ?-hydroxybutyrate and trace amounts of ?-hydroxyvalerate. The PHA synthesized under the conditions described did not differ from that synthesized by A. eutrophus cells from electrolytic hydrogen. В© 2001 MAIK "Nauka/Interperiodica".

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Altukhova, O.V.

Найти похожие

14.

The autotrophic synthesis of polyhydroxyalkanoate by Alcaligenes eutrophus in the presence of carbon monoxide [Text] / T. G. Volova, G. S. Kalacheva, O. V. Altukhova // Microbiology. - 2001. - Vol. 70, Is. 6. - P. 640-646, DOI 10.1023/A:1013175413013. - Cited References: 19 . - ISSN 0026-2617

РУБ Microbiology

Кл.слова (ненормированные):
hydrogen-oxidizing bacteria -- polyhydroxyalkanoates (PHAs) -- synthesis -- CO inhibition
Аннотация: The CO-resistant strain B5786 of the hydrogen-oxidizing bacterium Alcaligenes eutrophus was found to be able to synthesize polyhydroxyalkanoates (PHAs) under the conditions of growth limitation by nitrogen deficiency (the factor that promotes PHA synthesis) and growth inhibition by carbon monoxide. The gas mixtures that contained from 5 to 20 vol % CO did not inhibit the key enzymes of PHA synthesis-beta-ketothiolase. acetoacetyl-CoA reductase, hydroxybutyrate dehydrogenase, and PHA synthase. In the presence of CO, cells accumulated up to 70-75 wt % PHA (with respect to the dry biomass) without any noticeable increase in the consumption of the gas substrate. Chromatographic-mass spectrometric analysis showed that the PHA synthesized by A. eutrophus is a copolymer containing more than 99 mol % beta-hydroxybutyrate and trace amounts of beta-hydroxyvalerate. The PHA synthesized under the conditions described did not differ from that synthesized by A. eutrophus cells from electrolytic hydrogen.

WOS
Держатели документа:
Russian Acad Sci, Siberian Div, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Altukhova, O.V.

Найти похожие

15.

The architectonics of colonies of Bacillus subtilis 2335 [Text] / A. P. Puzyr' [et al.] // Microbiology. - 2002. - Vol. 71, Is. 1. - P. 57-64, DOI 10.1023/A:1017950316748. - Cited References: 12 . - ISSN 0026-2617

РУБ Microbiology

Кл.слова (ненормированные):
electron microscopy -- Bacillus subtills -- spores -- vegetative cells -- glycocalyx -- architectonics of bacterial colonies
Аннотация: Colonies grown from vegetative Bacillus subtilis 2335 cells had a standard structure, with bacillar cells occupying the whole colony volume. At the same time, the colonies of this bacterium grown from germinated spores had an abnormal structure characterized by the location of cells in a surface layer 100-200 mum thick at the colony boundary with the air. The glycocalyx of the colonies grown from spores was characterized by a wetting angle theta(e) of 120degrees-160degrees, whereas that of the colonies grown from vegetative cells had an angle theta(c) as low as 5degrees-30degrees. It is suggested that spores and vegetative cells follow different strategies of substrate colonization and that the architectonics of bacterial colonies is determined by the physicochemical properties of the glycocalyx.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Puzyr', A.P.; Mogil'naya, O.A.; Krylova, T.Y.; Popova, L.Y.

Найти похожие

16.

Synthesis of poly(3-hydroxybutyrate) by the autotrophic CO-oxidizing bacterium Seliberia carboxydohydrogena Z-1062 [Text] / T. Volova, N. Zhila, E. Shishatskaya // J. Ind. Microbiol. Biotechnol. - 2015. - Vol. 42, Is. 10. - P1377-1387, DOI 10.1007/s10295-015-1659-9. - Cited References:36. - The research was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences (project No 01201351505). . - ISSN 1367-5435. - ISSN 1476-5535

РУБ Biotechnology & Applied Microbiology
Рубрики:
CARBON-MONOXIDE
   RALSTONIA-EUTROPHA
   POLYHYDROXYALKANOATES
   GROWTH
   H-2
Кл.слова (ненормированные):
Carboxydobacteria -- Synthesis -- Poly(3-hydroxybutyrate) -- Carbon monoxide
Аннотация: The present study addresses growth parameters and physiological and biochemical characteristics of the aerobic CO-oxidizing carboxydobacterium Seliberia carboxydohydrogena Z-1062. Poly(3-hydroxybutyrate) yields were investigated in experiments with limiting concentrations of mineral nutrients (nitrogen or sulfur or nitrogen and sulfur) in batch culture of S. carboxydohydrogena Z-1062 grown on gas mixtures consisting of CO2, O-2, H-2, and CO. CO concentrations of 10, 20, and 30 % v/v did not affect polymer synthesis, whose content after 56-h cultivation under limiting concentrations of nitrogen and sulfur was 52.6-62.8 % of biomass weight at a productivity of 0.13-0.22 g/L h. The inhibitory effect of CO on cell concentration was revealed at CO concentration of 30 % v/v. That also caused a decrease in substrate (H-2 and O-2) use efficiency. Thus, this carboxydobacterium can be regarded as a potential producer of polyhydroxyalkanoates from industrial hydrogenous sources.

WOS,
Scopus
Держатели документа:
RAS, SB, Inst Biophys, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Volova, Tatiana; Zhila, Natalia; Shishatskaya, Ekaterina; Russian Academy of Sciences [01201351505]

Найти похожие

17.

Study of a Ralstonia eutropha culture producing polyhydroxyalkanoates on products of coal processing / T. G. Volova, N. A. Voinov // Applied Biochemistry and Microbiology. - 2004. - Vol. 40, Is. 3. - P249-252, DOI 10.1023/B:ABIM.0000025946.47013.03 . - ISSN 0003-6838
Кл.слова (ненормированные):
alkanoic acid -- carbon monoxide -- coal -- hydrogen -- lignite -- algorithm -- article -- bacterial strain -- bacterium culture -- coal gasification -- controlled study -- gas -- gas exchange -- mathematical computing -- nonhuman -- Ralstonia eutropha -- Bacteria (microorganisms) -- Ralstonia -- Wautersia eutropha
Аннотация: Kinetic indices of growth, polyhydroxyalkanoate (PHA) accumulation, and gas exchange were studied in a culture of the carbon monoxide-resistant hydrogen strain Ralstonia eutropha B-5786 grown on a gaseous substrate (GS) obtained by lignite gasification. The GS was shown to be suitable for PHA production. To increase the degree of GS consumption, various modes of gas supply to the culture were tested. Based on the results, an algorithm was developed for calculating and controlling gas-exchange parameters in the PHA-accumulating culture of Ralstonia eutropha, grown on a new GS allowing high polymer yields (up to 75%) and degrees of substrate utilization (up to 90%).

Scopus
Держатели документа:
Institute of Biophysics, Siberian Div. Russ. Acad. of Sci., Krasnoyarsk, 660036, Russian Federation
Siberian State Technol. University, Min. of Educ. of the Russ. Fed., Krasnoyarsk, 660049, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Voinov, N.A.

Найти похожие

18.

Species composition of winter bacterioplankton in two Siberian ponds determined by the 16s rRNA sequence analysis. / M. Y. Trusova, M. I. Gladyshev // Doklady Biological Sciences. - 2002. - Vol. 382. - P51-54 . - ISSN 0012-4966
Кл.слова (ненормированные):
bacterial RNA -- RNA 16S -- animal -- article -- bacterium -- classification -- ecosystem -- genetics -- microbiology -- molecular cloning -- molecular genetics -- plankton -- Russian Federation -- season -- species difference -- Animals -- Bacteria -- Cloning, Molecular -- Ecosystem -- Molecular Sequence Data -- Plankton -- RNA, Bacterial -- RNA, Ribosomal, 16S -- Seasons -- Siberia -- Species Specificity -- Water Microbiology

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Trusova, M.Y.; Gladyshev, M.I.

Найти похожие

19.

Species composition of winter bacterioplankton in blooming and nonblooming reservoirs as determined by 16S rRNA sequences / M. Yu. Trusova, M. I. Gladyshev // Doklady Biological Sciences. - 2005. - Vol. 405, Is. 1-6. - P443-445, DOI 10.1007/s10630-005-0160-4 . - ISSN 0012-4966
Кл.слова (ненормированные):
fresh water -- RNA 16S -- article -- bacterium -- classification -- genetic variability -- genetics -- isolation and purification -- microbiology -- molecular genetics -- phylogeny -- plankton -- Russian Federation -- season -- species difference -- Bacteria -- Fresh Water -- Molecular Sequence Data -- Phylogeny -- Plankton -- RNA, Ribosomal, 16S -- Seasons -- Siberia -- Species Specificity -- Variation (Genetics) -- Water Microbiology

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Krasnoyarsk State University, Krasnoyarsk, 660062, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Trusova, M.Yu.; Gladyshev, M.I.

Найти похожие

20.

Single-cell genomics-based analysis reveals a vital ecological role of thiocapsa sp. LSW in the meromictic Lake Shunet, Siberia / Y.-T. Wu, P.-W. Chiang, K. Tandon [et al.] // Microb. Genomics. - 2021. - Vol. 7, Is. 12. - Ст. 000712, DOI 10.1099/mgen.0.000712 . - ISSN 2057-5858
Кл.слова (ненормированные):
Flow cytometry -- Lake Shunet -- Purple sulfur bacteria -- Single-cell genomics -- genomic DNA -- RNA 16S -- Article -- bioinformatics -- carbon metabolism -- Enterobacter -- fluorescence activated cell sorting -- gene amplification -- gene ontology -- high throughput sequencing -- metagenomics -- microbial community -- microbial diversity -- molecular genetics -- nitrogen metabolism -- nonhuman -- nucleotide sequence -- phylogenetic tree -- phylogeny -- polymerase chain reaction -- Sanger sequencing -- Thiocapsa
Аннотация: Meromictic lakes usually harbour certain prevailing anoxygenic phototrophic bacteria in their anoxic zone, such as the purple sulfur bacterium (PSB) Thiocapsa sp. LSW (hereafter LSW) in Lake Shunet, Siberia. PSBs have been suggested to play a vital role in carbon, nitrogen and sulfur cycling at the oxic–anoxic interface of stratified lakes; however, the ecological significance of PSBs in the lake remains poorly understood. In this study, we explored the potential ecological role of LSW using a deep-sequencing analysis of single-cell genomics associated with flow cytometry. An approximately 2.7 Mb draft genome was obtained based on the co-assembly of five single-cell genomes. LSW might grow photolithoautotrophically and could play putative roles not only as a carbon fixer and diazotroph, but also as a sulfate reducer/oxidizer in the lake. This study provides insights into the potential ecological role of Thiocapsa sp. in meromictic lakes. © 2021 The Authors.

Scopus
Держатели документа:
Department of Forestry, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan
Biodiversity Research Center, Academia Sinica, Taipei, 115, Taiwan
Institute of Biophysics, Siberian Division of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Wu, Y. -T.; Chiang, P. -W.; Tandon, K.; Rogozin, D. Y.; Degermendzhy, A. G.; Tang, S. -L.

Найти похожие