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1.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Rodionova N.S., Petushkov V.N.
Заглавие : Effect of different salts and detergents on luciferin-luciferase luminescence of the enchytraeid Fridericia heliota
Место публикации : Journal of Photochemistry and Photobiology B: Biology. - 2006. - Vol. 83, Is. 2. - С. 123-128. - ISSN 10111344 (ISSN) , DOI 10.1016/j.jphotobiol.2005.12.014
Ключевые слова (''Своб.индексиров.''): atp--bioluminescence--earthworms--ions--luciferin-luciferase systems--triton x-100--adenosine triphosphate--anion--bromine--calcium ion--carbonic acid--cation--chloride--chromium derivative--detergent--dodecyl sulfate sodium--inorganic salt--iodine--iron derivative--luciferase--luciferin--magnesium ion--manganese--nitrate--phosphate--sulfate--sulfite--triton x 100--annelid worm--article--bioluminescence--concentration (parameters)--controlled study--enzyme activation--enzyme activity--enzyme inhibition--enzyme mechanism--in vitro study--nonhuman--priority journal--qualitative analysis--quantitative analysis--adenosine triphosphate--animals--cations, divalent--cations, monovalent--detergents--firefly luciferin--kinetics--luciferases--luminescence--metals--oligochaeta--photobiology--salts--annelida--clitellata--earthworms (sp.)--enchytraeidae--fridericia heliota--oligochaeta (metazoa)--pheretima sieboldi
Аннотация: The study addresses the effect produced by different inorganic salts and detergents (SDS, Triton X-100, the Tween series) on the ATP-dependent bioluminescent reaction catalyzed by the luciferase of the new earthworm species Fridericia heliota (Annelida: Clitellata: Oligochaeta: Enchytraeidae). It has been shown that the effect of divalent metal salts on luminescence is determined by the action of cations. Three of them - Mg2+, Mn2+ and Ca2+ - can stimulate luciferase activity at concentrations varying within a wide range, and Mn2+ can act as a 100%-effective substitute for Mg2+ in F. heliota luminescence reaction in vitro. The inhibitory effect of monovalent metal salts on luminescence is largely determined by the action of the anion part of the molecule. The effectiveness of the inhibitory effect of anions increases in the following order: {Mathematical expression}. Of the sodium salts, dodecyl sulfate, which is an anionic detergent, produces the strongest inhibitory effect on luciferase. On the contrary, nonionic detergents produce a stimulatory effect on the F. heliota luciferase. The action of the most effective of them - Triton X-100 - is determined by its ability to reduce the actual concentration of lipid inhibitors in the reaction mixture. В© 2006 Elsevier B.V. All rights reserved.
Scopus
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2.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Rodionova N.S., Bondar V.S., Petushkov V.N.
Заглавие : Ca(2+)-activator of the luminescence system of the earthworms Henlea sp., (Annelida: Clitellata: Oligochaeta: Enchytraeidae)
Место публикации : Doklady. Biochemistry and biophysics. - 2002. - Vol. 386. - С. 260-263. - ISSN 16076729 (ISSN)
Ключевые слова (''Своб.индексиров.''): calcium--divalent cation--edetic acid--luciferase--luciferin--metal--animal--annelid worm--article--chemistry--dose response--enzymology--genetics--kinetics--luminescence--metabolism--animals--calcium--cations, divalent--dose-response relationship, drug--edetic acid--firefly luciferin--kinetics--luciferases--luminescent measurements--metals--oligochaeta
Scopus
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3.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kydryasheva N.S., Zuzikova E.V., Gutnik T.V.
Заглавие : Mechanism of action of metal salts on bacterial bioluminescent system in vitro
Колич.характеристики :7 с
Место публикации : Biofizika: MEZHDUNARODNAYA KNIGA, 1999. - Vol. 44, Is. 2. - P244-250. - ISSN 0006-3029
Примечания : Cited References: 21
Ключевые слова (''Своб.индексиров.''): bacterial bioluminescent system--metal salts, mechanism of action
Аннотация: The action of met al salts on the luciferase-NADH: FMN-oxydoheductase bacterial luminescent system in vitro was studied. The effect is shown to depend on the nature of cations rather than anions, The type of the effect (activation, inhibition, and absence of the effect) is determined by the structure of the; cation, the nature and occupancy of its external orbitals, and charge. The results were interpreted on the basis of the hypothesis that upper electron excited states of the emitter are involved in the bioluminescence process. Based on thermodynamic calculations, the type of the effect was related to the energetic characteristics of the cation, Presumably, the mechanism of action of cations is determined by their involvement in the formation and deactivation of electron excited states in the bioluminescence process.
WOS
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