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Creation of artificial luciferases to expand their analytical potential / L. A. Frank // Comb. Chem. High Throughput Screen. - 2015. - Vol. 18, Is. 10. - P919-929 . - ISSN 1386-2073
Кл.слова (ненормированные):
Bioimaging -- Bioluminescence -- Luciferase -- Luciferase-based assay -- Luciferin -- Mutagenesis -- Photoprotein -- Reporter assay
Аннотация: Bioluminescent proteins have been intensively used as high sensitive reporters in all kinds of binding assays (immuno-, nucleic acid hybridization assays, etc.) and in bioimaging. But natural luciferases do not always meet the requirements set for them as the assay reporters: thermostabitity, definite bioluminescence spectral and kinetics characteristics, stability to chemical modifications, etc. Luciferases with different appropriate characteristics as well as various luciferin derivatives were obtained using mutagenesis and chemical synthesis. Thanks to rigorous efforts of many researchers bioluminescencebased analytical techniques offer a great potential for solving analytical tasks in the field of biotechnology, biomedicine, pharmacology, etc. © 2015 Bentham Science Publishers.

Scopus
Держатели документа:
Photobiology Laboratory, Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russian Federation
Siberian Federal University, Svobodnii ave., 79, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Frank, L. A.

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Recent trends and advancements in bioassay based on bioluminescent technologies / L. A. Frank // Comb. Chem. High Throughput Screen. - 2015. - Vol. 18, Is. 10. - P918 . - ISSN 1386-2073

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch 50/50, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Frank, L. A.

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Simultaneous genotyping of four single nucleotide polymorphisms associated with risk factors of hemostasis disorders / E. E. Bashmakova, V. V. Krasitskaya, L. A. Frank // Comb. Chem. High Throughput Screen. - 2015. - Vol. 18, Is. 10. - P930-936 . - ISSN 1386-2073
Кл.слова (ненормированные):
Bioluminescent microassay -- Multiplex PCR -- PEXT reaction -- Photoprotein obelin -- SNP detection
Аннотация: Multiplex simultaneous genotyping technique was developed for four polymorphisms in genes coding for blood coagulation factors and homocysteine metabolism which are considered as thrombophilia related mutations: FV Leiden, FII G20210A, MTHFR C677T, and FVII G10976A. It is based on primer extension reaction with the following bioluminescent solid-phase microassay. At that, two different in bioluminescence obelin mutants were applied to simultaneous detection of two gene allelic variants. The assay is carried out in microtiter plate format and provides fast and reliable genotyping of four single nucleotide polymorphisms in four different genes within 2.5 hours. A large number of clinical samples were analyzed and the obtained results were found to be in complete correlation with those obtained by using conventional RT-PCR techniques. © 2015 Bentham Science Publishers.

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Держатели документа:
Photobiology Laboratory, Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russian Federation
Siberian Federal University, Svobodnii Ave., 79, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Bashmakova, E. E.; Krasitskaya, V. V.; Frank, L. A.

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On the mechanism of biological activation by tritium / T. V. Rozhko [et al.] // J. Environ. Radioact. - 2016. - Vol. 157. - P131-135, DOI 10.1016/j.jenvrad.2016.03.017 . - ISSN 0265-931X
Кл.слова (ненормированные):
DNA mutations -- Low-dose effect -- Luminous marine bacteria -- Radiation hormesis -- Tritium
Аннотация: The mechanism of biological activation by beta-emitting radionuclide tritium was studied. Luminous marine bacteria were used as a bioassay to monitor the biological effect of tritium with luminescence intensity as the physiological parameter tested. Two different types of tritium sources were used: HTO molecules distributed regularly in the surrounding aqueous medium, and a solid source with tritium atoms fixed on its surface (tritium-labeled films, 0.11, 0.28, 0.91, and 2.36 MBq/cm2). When using the tritium-labeled films, tritium penetration into the cells was prevented. The both types of tritium sources revealed similar changes in the bacterial luminescence kinetics: a delay period followed by bioluminescence activation. No monotonic dependences of bioluminescence activation efficiency on specific radioactivities of the films were found. A 15-day exposure to tritiated water (100 MBq/L) did not reveal mutations in bacterial DNA. The results obtained give preference to a "non-genomic" mechanism of bioluminescence activation by tritium. An activation of the intracellular bioluminescence process develops without penetration of tritium atoms into the cells and can be caused by intensification of trans-membrane cellular processes stimulated by ionization and radiolysis of aqueous media. © 2016 Elsevier Ltd.

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Держатели документа:
Krasnoyarsk State Medical Academy, P.Zheleznyaka 1, Krasnoyarsk, Russian Federation
Siberian Federal University, Svobodny 79, Krasnoyarsk, Russian Federation
Moscow State University, Department of Chemistry, Moscow, Russian Federation
Institute of Biophysics SB RAS, Akademgorodok 50/50, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Rozhko, T. V.; Badun, G. A.; Razzhivina, I. A.; Guseynov, O. A.; Guseynova, V. E.; Kudryasheva, N. S.

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Applications of luminous bacteria enzymes in toxicology / V. A. Kratasyuk, E. N. Esimbekova // Comb. Chem. High Throughput Screen. - 2015. - Vol. 18, Is. 10. - P952-959 . - ISSN 1386-2073
Кл.слова (ненормированные):
Bioluminescence -- Bioluminescent toxicity enzymatic assay -- Immobilization of enzymes -- Luciferase -- Total toxicity
Аннотация: This review describes the principle and applications of bioluminescent enzymatic toxicity bioassays. This type of assays uses bacterial coupled enzyme systems: NADH:FMN-oxidoreductase and luciferase to replace living organisms in developing cost-competitive biosensors for environmental, medical and industrial applications. These biosensors instantly signal chemical and biological hazards and allow for detecting a great amount of toxic compounds with advantages associated with fast results, high sensitivity, simplicity, low cost and safety of the procedure. © 2015 Bentham Science Publishers.

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Держатели документа:
Siberian Federal University, Svobodnii Ave., 79, Krasnoyarsk, Russian Federation
Photobiology Laboratory, Russian Academy of Sciences, Siberian Branch, Institute of Biophysics SB RAS, Akademgorodok 50/50, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Kratasyuk, V. A.; Esimbekova, E. N.

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Recent Trends and Advancements in Bioassay Based on Bioluminescent Technologies [Text] / L. A. Frank // Comb. Chem. High Throughput Screen. - 2015. - Vol. 18, Is. 10. - P918-918, DOI 10.2174/138620731810151103112033. - Cited References:0 . - ISSN 1386-2073. - ISSN 1875-5402

РУБ Biochemical Research Methods + Chemistry, Applied + Pharmacology &

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia.
Доп.точки доступа:
Frank, Ludmila A.

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Creation of Artificial Luciferases to Expand their Analytical Potential [Text] / L. A. Frank // Comb. Chem. High Throughput Screen. - 2015. - Vol. 18, Is. 10. - P919-929, DOI 10.2174/1386207318666150917100011. - Cited References:79. - The work was supported by: the RFBR grant No. 14-08-00902/14; the State budget allocated to the fundamental research at the Russian Academy of Sciences (project No. VI 57.1.1). . - ISSN 1386-2073. - ISSN 1875-5402

РУБ Biochemical Research Methods + Chemistry, Applied + Pharmacology &
Рубрики:
BIOLUMINESCENT REPORTER APPLICATIONS
COELENTERAZINE-BINDING PROTEIN
Кл.слова (ненормированные):
Luciferase -- luciferin -- photoprotein -- bioluminescence -- mutagenesis -- luciferase-based assay -- bioimaging -- reporter assay
Аннотация: Bioluminescent proteins have been intensively used as high sensitive reporters in all kinds of binding assays (immuno-, nucleic acid hybridization assays, etc.) and in bioimaging. But natural luciferases do not always meet the requirements set for them as the assay reporters: thermostabitity, definite bioluminescence spectral and kinetics characteristics, stability to chemical modifications, etc. Luciferases with different appropriate characteristics as well as various luciferin derivatives were obtained using mutagenesis and chemical synthesis. Thanks to rigorous efforts of many researchers bioluminescence-based analytical techniques offer a great potential for solving analytical tasks in the field of biotechnology, biomedicine, pharmacology, etc.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Photobiol Lab, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Frank, Ludmila A.; RFBR [14-08-00902/14]; [VI 57.1.1]

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Semisynthetic photoprotein reporters for tracking fast Ca2+ transients / N. P. Malikova, A. J. Borgdorff, E. S. Vysotski // Photochem. Photobiol. Sci. - 2015. - Vol. 14, Is. 12. - P2213-2224, DOI 10.1039/c5pp00328h . - ISSN 1474-905X
Аннотация: Changes in the intracellular concentration of free ionized calcium ([Ca2+]i) control a host of cellular processes as varied as vision, muscle contraction, neuronal signal transmission, proliferation, apoptosis etc. The disturbance in Ca2+-signaling causes many severe diseases. To understand the mechanisms underlying the control by calcium and how disorder of this regulation relates to pathological conditions, it is necessary to measure [Ca2+]i. The Ca2+-regulated photoproteins which are responsible for bioluminescence of marine coelenterates have been successfully used for this purpose over the years. Here we report the results on comparative characterization of bioluminescence properties of aequorin from Aequorea Victoria, obelin from Obelia longissima, and clytin from Clytia gregaria charged by native coelenterazine and coelenterazine analogues f, i, and hcp. The comparison of specific bioluminescence activity, stability, emission spectra, stopped-flow kinetics, sensitivity to calcium, and effect of physiological concentrations of Mg2+ establishes obelin-hcp as an excellent semisynthetic photoprotein to keep track of fast intracellular Ca2+ transients. The rate of rise of its light signal on a sudden change of [Ca2+] is almost 3- and 11-fold higher than those of obelin and aequorin with native coelenterazine, respectively, and 20 times higher than that of the corresponding aequorin-hcp. In addition, obelin-hcp preserves a high specific bioluminescence activity and displays higher Ca2+-sensitivity as compared to obelin charged by native coelenterazine and sensitivity to Ca2+ comparable with those of aequorin-f and aequorin-hcp. © The Royal Society of Chemistry and Owner Societies 2015.

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Держатели документа:
Photobiology Laboratory, Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russian Federation
Institut des Neurosciences Alfred Fessard, UPR 3294, Centre National de la Recherche Scientifique, Avenue de la Terrasse, Gif-sur-Yvette, France

Доп.точки доступа:
Malikova, N. P.; Borgdorff, A. J.; Vysotski, E. S.
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Surface wettability and energy effects on the biological performance of poly-3-hydroxybutyrate films treated with RF plasma / D. S. Syromotina [et al.] // Mater. Sci. Eng. C. - 2016. - Vol. 62. - P450-457, DOI 10.1016/j.msec.2016.01.075 . - ISSN 0928-4931
Кл.слова (ненормированные):
Plasma -- Polar component -- poly-3-hydroxybutyrate membranes -- Surface energy -- Surface treatment -- Ammonia -- Biodegradable polymers -- Cell adhesion -- Contact angle -- Free energy -- Interfacial energy -- Plasma applications -- Plasmas -- Surface chemistry -- Surface properties -- Surface roughness -- Surface topography -- Wetting -- Biological performance -- Crystalline structure -- Polar components -- Poly-3-hydroxybutyrate -- Surface free energy -- Surface roughness changes -- Surface wettability -- Water contact angle -- Surface treatment
Аннотация: The surface properties of poly-3-hydroxybutyrate (P3HB) membranes were modified using oxygen and an ammonia radio-frequency (RF, 13.56 MHz) plasma. The plasma treatment procedures used in the study only affected the surface properties, including surface topography, without inducing any significant changes in the crystalline structure of the polymer, with the exception being a power level of 250 W. The wettability of the modified P3HB surfaces was significantly increased after the plasma treatment, irrespective of the treatment procedure used. It was revealed that both surface chemistry and surface roughness changes caused by the plasma treatment affected surface wettability. A treatment-induced surface aging effect was observed and resulted in an increase in the water contact angle and a decrease in the surface free energy. However, the difference in the water contact angle between the polymers that had been treated for 4 weeks and the untreated polymer surfaces was still significant. A dependence between cell adhesion and proliferation and the polar component of the surface energy was revealed. The increase in the polar component after the ammonia plasma modification significantly increased cell adhesion and proliferation on biodegradable polymer surfaces compared to the untreated P3HB and the P3HB modified using an oxygen plasma. © 2016 Elsevier B.V.

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Держатели документа:
Department of Experimental Physics, National Research Tomsk Polytechnic University, Tomsk, Russian Federation
Fraunhofer Institute for Interfacial Engineering and Biotechnology, IGB, Stuttgart, Germany
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, 50/50 Akademgorodok, Krasnoyarsk, Russian Federation
School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodny pr., Krasnoyarsk, Russian Federation
Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Essen, Germany
Technical Chemistry II and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Essen, Germany

Доп.точки доступа:
Syromotina, D. S.; Surmenev, R. A.; Surmeneva, M. A.; Boyandin, A. N.; Nikolaeva, E. D.; Prymak, O.; Epple, M.; Ulbricht, M.; Oehr, C.; Volova, T. G.

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10.

Ni2+-zeolite/ferrosphere and Ni2+-silica/ferrosphere beads for magnetic affinity separation of histidine-tagged proteins / T. A. Vereshchagina [et al.] // Dalton Trans. - 2016. - Vol. 45, Is. 4. - P1582-1592, DOI 10.1039/c5dt03827h . - ISSN 1477-9226
Кл.слова (ненормированные):
Alkalinity -- Amino acids -- Chemical modification -- Fly ash -- Hydrothermal synthesis -- Ion exchange -- Magnetic separation -- Magnetism -- Proteins -- Silica -- Zeolites -- Conventional methods -- Core shell structure -- Green fluorescent protein -- Histidine-tagged proteins -- Hydrothermal treatments -- Magnetic affinity -- Mesoporous Silica -- Sorption capacities -- Nickel
Аннотация: Magnetic Ni2+-zeolite/ferrosphere and Ni2+-silica/ferrosphere beads (Ni-ferrosphere beads - NFB) of a core-shell structure were synthesized starting from coal fly ash ferrospheres having diameters in the range of 0.063-0.050 mm. The strategy of NFB fabrication is an oriented chemical modification of the outer surface preserving the magnetic core of parent beads with the formation of micro-mesoporous coverings. Two routes of ferrosphere modification were realized, such as (i) hydrothermal treatment in an alkaline medium resulting in a NaP zeolite layer and (ii) synthesis of micro-mesoporous silica on the glass surface using conventional methods. Immobilization of Ni2+ ions in the siliceous porous shell of the magnetic beads was carried out via (i) the ion exchange of Na+ for Ni2+ in the zeolite layer or (ii) deposition of NiO clusters in the zeolite and silica pores. The final NFB were tested for affinity in magnetic separation of the histidine-tagged green fluorescent protein (GFP) directly from a cell lysate. Results pointed to the high affinity of the magnetic beads towards the protein in the presence of 10 mM EDTA. The sorption capacity of the ferrosphere-based Ni-beads with respect to GFP was in the range 1.5-5.7 mg cm-3. © The Royal Society of Chemistry.

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Держатели документа:
Institute of Chemistry and Chemical Technology SB RAS, 50/24 Akademgorodok, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, 50/50 Akademgorodok, Krasnoyarsk, Russian Federation
Siberian Federal University, 79 Svobodnyi Avenue, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Vereshchagina, T. A.; Fedorchak, M. A.; Sharonova, O. M.; Fomenko, E. V.; Shishkina, N. N.; Zhizhaev, A. M.; Kudryavtsev, A. N.; Frank, L. A.; Anshits, A. G.

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11.

Role of certain amino acid residues of the coelenterazine-binding cavity in bioluminescence of light-sensitive Ca2+-regulated photoprotein berovin / L. P. Burakova [et al.] // Photochem. Photobiol. Sci. - 2016. - Vol. 15, Is. 5. - P691-704, DOI 10.1039/c6pp00050a . - ISSN 1474-905X
Аннотация: Bright bioluminescence of ctenophores is caused by Ca2+-regulated photoproteins. Although these photoproteins are functionally identical to and share many properties of cnidarian photoproteins, like aequorin and obelin, and retain the same spatial architecture, they are extremely sensitive to light, i.e. lose the ability to bioluminesce on exposure to light over the entire absorption spectrum. In addition, the degree of identity of their amino acid sequences with those of cnidarian photoproteins is only 29.4%. This suggests that the residues involved in bioluminescence of ctenophore and cnidarian photoproteins significantly differ. Here we describe the bioluminescent properties of berovin mutants with substitution of the residues located in the photoprotein internal cavity. Since the spatial structure of berovin bound with a substrate is not determined yet, to identify these residues we have modeled it with an accommodated substrate using the structures of some cnidarian Ca2+-regulated photoproteins with bound coelenterazine or coelenteramide as templates in order to obtain an adequate sampling and to take into account all possible conformers and variants for ligand-protein docking. Based on the impact of substitutions on the bioluminescent properties and model structures we speculate that within the internal cavity of ctenophore photoproteins, coelenterazine is bound as a 2-peroxy anion adduct which is stabilized owing to Coulomb interaction with a positively charged guanidinium group of Arg41 paired with Tyr204. In this case, the bioluminescence reaction is triggered by only calcium-induced conformational changes leading to the disturbance of charge-charge interaction. © 2016 The Royal Society of Chemistry and Owner Societies.

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Держатели документа:
Photobiology Laboratory, Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Burakova, L. P.; Stepanyuk, G. A.; Eremeeva, E. V.; Vysotski, E. S.

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12.

The effect of silver ions electrolytically introduced into colloidal nanodiamond solution on its viscosity and thermal conductivity / A. P. Puzir’ [et al.] // Colloid J. - 2017. - Vol. 79, Is. 2. - P258-263, DOI 10.1134/S1061933X17020119 . - ISSN 1061-933X
Кл.слова (ненормированные):
Dispersions -- Ions -- Metal ions -- Nanodiamonds -- Nanoparticles -- Silver -- Viscosity -- A-stable -- Detonation nanodiamond -- Diamond nano-particles -- Effect of silvers -- Silver concentration -- Silver ions -- Thermal conductivity
Аннотация: Experimental data have been presented on the influence of silver on the viscosity and thermal conductivity of a dispersion of diamond nanoparticles. A stable dispersion (5 wt %) of detonation nanodiamond particles has been used in the experiments. Silver ions have been introduced electrolytically into the dispersion of diamond nanoparticles. Silver concentration was not higher than 0.05 wt %. It has been shown that the introduction of silver ions significantly affects the thermal conductivity and viscosity of the dispersion. © 2017, Pleiades Publishing, Ltd.

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Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, ul. Akademgorodok 50/50, Krasnoyarsk, Russian Federation
Siberian Federal University, Svobodnyi pr. 79., Krasnoyarsk, Russian Federation
Special Design and Technology Bureau Nauka, Institute of Computational Technologies, Siberian Branch, Russian Academy of Sciences, pr. Mira 53, Krasnoyarsk, Russian Federation
Kirensky Institute of Physics, Siberian Branch, Russian Academy of Sciences, ul. Akademgorodok 50/38, Krasnoyarsk, Russian Federation
Institute of Chemistry and Chemical Technology, Siberian Branch, Russian Academy of Sciences, ul. Akademgorodok 50/24, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Puzir’, A. P.; Minakov, A. V.; Burov, A. E.; Zharkov, S. M.; Maksimov, N. G.; Pryazhnikov, M. I.

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13.

Progress in the Study of Bioluminescent Earthworms / N. S. Rodionova [et al.] // Photochem. Photobiol. - 2017. - Vol. 93, Is. 2. - P416-428, DOI 10.1111/php.12709 . - ISSN 0031-8655
Аннотация: Even though bioluminescent oligochaetes rarely catch people's eyes due to their secretive lifestyle, glowing earthworms sighting reports have come from different areas on all continents except Antarctica. A major breakthrough in the research of earthworm bioluminescence occurred in the 1960s with the studies of the North American Diplocardia longa. Comparative studies conducted on 13 earthworm species belonging to six genera showed that N-isovaleryl-3-aminopropanal (Diplocardia luciferin) is the common substrate for bioluminescence in all examined species, while luciferases appeared to be responsible for the color of bioluminescence. The second momentous change in the situation has occurred with the discovery in Siberia (Russia) of two unknown luminous enchytraeids. The two bioluminescent systems belong to different types, have different spectral characteristics and localization, and different temperature and pH optima. They are unique, and this fact is confirmed by the negative results of all possible cross-reactions. The bioluminescent system of Henlea sp. comprises four essential components: luciferase, luciferin, oxygen and calcium ion. For Friderica heliota, the luminescent reaction requires five components: luciferase, luciferin, ATP, magnesium ion and oxygen. Along with luciferin, more than a dozen analogues were isolated from worm biomass. These novel peptide-like natural compounds represent an unprecedented chemistry found in terrestrial organisms. © 2017 The American Society of Photobiology

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Держатели документа:
Laboratory of Photobiology, Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
Department of Physics, Earth and Environmental Sciences, University of Siena, Siena, Italy
Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation
Pirogov Russian National Research Medical University, Moscow, Russian Federation

Доп.точки доступа:
Rodionova, N. S.; Rota, E.; Tsarkova, A. S.; Petushkov, V. N.

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14.

Bioluminescent detection of tick-borne encephalitis virus in native ticks / A. N. Kudryavtsev, L. P. Burakova, L. A. Frank // Anal. Methods. - 2017. - Vol. 9, Is. 15. - P2252-2255, DOI 10.1039/c7ay00535k . - ISSN 1759-9660
Кл.слова (ненормированные):
Proteins -- Recombinant proteins -- Viruses -- Binding proteins -- Bioluminescence
Аннотация: A one-step bioluminescent immunoassay for tick-borne encephalitis virus (TBEV) in natural ticks based on the hybrid protein 14D5a-Rm7 was developed. Recombinant Ca2+-dependent coelenterazine-binding protein was shown to be a more convenient substrate form for the Rm7 domain than coelenterazine. Over 600 samples of natural ticks were analyzed and shown to have essential differences in the discrimination factor D for TBEV-positive (2.77 ± 0.81) and TBEV-negative (1.15 ± 0.28) samples. © The Royal Society of Chemistry.

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Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Kudryavtsev, A. N.; Burakova, L. P.; Frank, L. A.

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15.

Fluorescent coelenteramide-containing protein as a color bioindicator for low-dose radiation effects / A. S. Petrova [et al.] // Anal. Bioanal. Chem. - 2017. - Vol. 409, Is. 18. - P4377-4381, DOI 10.1007/s00216-017-0404-9. - Cited References:22. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences (project 01201351504) and by the Russian Foundation for Basic Research, Grant No. 16-34-00695. . - ISSN 1618-2642. - ISSN 1618-2650

РУБ Biochemical Research Methods + Chemistry, Analytical
Рубрики:
LUMINOUS MARINE-BACTERIA
DISCHARGED-OBELIN
AEQUORIN
Кл.слова (ненормированные):
Fluorescent protein -- Coelenteramide -- Discharged photoprotein obelin -- Multicolor bioindicator -- Radiotoxicity
Аннотация: The study addresses the application of fluorescent coelenteramide-containing proteins as color bioindicators for radiotoxicity evaluation. Biological effects of chronic low-dose radiation are under investigation. Tritiated water (200 MBq/L) was used as a model source of low-intensive ionizing radiation of beta type. 'Discharged obelin,' product of bioluminescent reaction of marine coelenterate Obelia longissimi, was used as a representative of the coelenteramide-containing proteins. Coelenteramide, fluorophore of discharged obelin, is a photochemically active molecule; it produces fluorescence forms of different color. Contributions of 'violet' and 'blue-green' forms to the visible fluorescence serve as tested parameters. The contributions depend on the coelenteramide's microenvironment in the protein, and, hence, evaluate distractive ability and toxicity of radiation. The protein samples were exposed to beta radiation for 18 days, and maximal dose accumulated by the samples was 0.28 Gy, being close to a tentative limit of a low-dose interval. Increase of relative contribution of 'violet' fluorescence under exposure to the beta irradiation was revealed. High sensitivity of the protein-based test system to low-dose ionizing radiation (to 0.03 Gy) was demonstrated. The study develops physicochemical understanding of radiotoxic effects.

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Держатели документа:
FRC KSC SB RAS, Inst Biophys SB RAS, Akademgorodok 50, Krasnoyarsk 660036, Russia.
Krasnoyarsk State Agrarian Univ, Krasnoyarsk 660049, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
Moscow MV Lomonosov State Univ, Moscow 119991, Russia.

Доп.точки доступа:
Petrova, Alena S.; Lukonina, Anna A.; Badun, Gennadii A.; Kudryasheva, Nadezhda S.; Russian Academy of Sciences [01201351504]; Russian Foundation for Basic Research [16-34-00695]

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16.

Inhibition effect of food preservatives on endoproteinases / E. N. Esimbekova [et al.] // Food Chem. - 2017. - Vol. 235. - P294-297, DOI 10.1016/j.foodchem.2017.05.059 . - ISSN 0308-8146
Кл.слова (ненормированные):
Endoproteinases -- Food additives -- Pancreatic disease -- Pancreatic enzymes -- Benzoic acid -- Enzyme activity -- Enzymes -- Food additives -- Food preservatives -- Potassium sorbate -- Sodium -- Acceptable daily intakes -- Decay constants -- Endoproteinases -- Human metabolisms -- Inhibition effect -- Light intensity -- Protein digestion -- Sodium benzoate -- Sorbic acid
Аннотация: The present manuscript proposes a novel approach to assess the impact of food additives on human metabolism by analysing their effect on biomarker enzyme activity. Alterations in the activity of pancreatic enzymes, such as chymotrypsin and trypsin, which are affected by the most common food preservatives, sodium benzoate (E211), potassium sorbate (E202) and sorbic acid (E200), have been evaluated. The proteinase activity was analysed with a bioluminescent method using the light intensity decay constant. Our study revealed that the preservatives reduce proteinase activity by 50% (EC50) at a much lower concentration than their acceptable daily intake (ADI). Thus, sodium benzoate and sorbic acid have an inhibition effect on chymotrypsin at concentrations 14 times lower and 70 times lower than their ADI and this increases with exposure time. Food preservative consumption impacts negatively on protein digestion, which is especially dangerous for patients with pancreatitis. © 2017 Elsevier Ltd

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Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, Krasnoyarsk, Russian Federation
Siberian Federal University, Institute of Fundamental Biology and Biotechnology, Krasnoyarsk, Russian Federation
Krasnoyarsk State Agricultural University, Institute of Agro-ecological Technologies, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Esimbekova, E. N.; Asanova, A. A.; Deeva, A. A.; Kratasyuk, V. A.

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17.

Triploidy does not decrease contents of eicosapentaenoic and docosahexaenoic acids in filets of pink salmon Oncorhynchus gorbuscha / M. I. Gladyshev [et al.] // Food Chem. - 2017. - Vol. 216. - P66-69, DOI 10.1016/j.foodchem.2016.08.021 . - ISSN 0308-8146
Кл.слова (ненормированные):
Essential fatty acids -- Filets -- Triploid fish -- Aquaculture -- Fish -- Polyunsaturated fatty acids -- Unsaturated fatty acids -- Docosahexaenoic acid -- Eicosapentaenoic acid -- Essential fatty acids -- Fa compositions -- Filets -- Muscle tissues -- Pink salmon -- White sea -- Fatty acids
Аннотация: Triploid fish has become an important item of commercial aquaculture, but data on its fatty acid (FA) composition are still controversial, especially regarding essential polyunsaturated fatty acids, eicosapentaenoic acid (20:5n-3, EPA) and docosahexaenoic acid (22:6n-3, DHA). We studied FA composition and content of diploid and triploid pink salmon Oncorhynchus gorbuscha, reared in aquaculture in a bay of the White Sea (Russia). FA composition, measured as percentages of total FA of triploids and immature diploid females significantly differed from that of mature diploid fish. Specifically, mature diploids had higher percentage of EPA and DHA in their muscle tissue (filets) compared to that of triploids and immature diploid females. Nevertheless, the contents of EPA and DHA per mass of the filets in diploid and triploid specimens were similar. Thus, no special efforts are needed to improve EPA and DHA contents in filets of triploids. © 2016 Elsevier Ltd

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Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation
Siberian Federal University, Svobodny av. 79, Krasnoyarsk, Russian Federation
A. N. Severtsov Institute of Ecology and Evolution of Russian Academy of Sciences, Leninsky Prospect, 33, Moscow, Russian Federation

Доп.точки доступа:
Gladyshev, M. I.; Artamonova, V. S.; Makhrov, A. A.; Sushchik, N. N.; Kalachova, G. S.; Dgebuadze, Y. Y.

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18.

Constructing Slow-Release Formulations of Metribuzin Based on Degradable Poly(3-hydroxybutyrate) / A. N. Boyandin [et al.] // J. Agric. Food Chem. - 2016. - Vol. 64, Is. 28. - P5625-5632, DOI 10.1021/acs.jafc.5b05896 . - ISSN 0021-8561
Кл.слова (ненормированные):
controlled release -- degradable poly(3-hydroxybutyrate) -- herbicide -- metribuzin -- release kinetics -- Polyethylene glycols -- Weed control -- Controlled release -- Environmental release -- Herbicide release -- Laboratory system -- Matrix formulation -- Metribuzin -- Poly-3-hydroxybutyrate -- Release kinetics -- Herbicides
Аннотация: Experimental formulations of herbicide metribuzin embedded in matrices of degradable natural polymer poly(3-hydroxybutyrate) (P3HB) and its composites with poly(ethylene glycol) (PEG), poly-?-caprolactone (PCL), and wood powder have been prepared in the form of pressed pellets containing 75% polymeric basis (pure P3HB or its composite with a second component at a ratio of 7:3) and 25% metribuzin. Incubation of formulations in soil laboratory systems led to the degradation of the matrix and herbicide release. The most active release of metribuzin (about 60% of the embedded herbicide over 35 days) was detected for the P3HB/PEG carrier compared to the P3HB, P3HB/wood, and P3HB/PCL forms (30-40%). Thus, the study shows that herbicide release can be controlled by the matrix formulation. Metribuzin formulations exerted a significant herbicidal effect on the plant Agrostis stolonifera, used as a weed plant model. Application of these long-term formulations will make it possible to reduce environmental release of chemicals, which will restrict the rate of their accumulation in trophic chains of ecosystems and abate their adverse effects on the biosphere. © 2016 American Chemical Society.

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Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, 50/50 Akademgorodok, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Boyandin, A. N.; Zhila, N. O.; Kiselev, E. G.; Volova, T. G.

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19.

Properties of a novel quaterpolymer P(3HB/4HB/3HV/3HHx) / T. G. Volova [et al.] // Polymer. - 2016. - Vol. 101. - P67-74, DOI 10.1016/j.polymer.2016.08.048 . - ISSN 0032-3861
Кл.слова (ненормированные):
Physicochemical and mechanical properties -- Polyhydroxyalkanoates -- Quaterpolymers -- Biocompatibility -- Cell culture -- Chain length -- Chains -- Decomposition -- 3-Hydroxyhexanoate -- Degrees of crystallinity -- Elongation at break -- Physico-chemical and mechanical properties -- Polyhydroxyalkanoates -- Quaterpolymers -- Short chain lengths -- Thermal decomposition temperature -- Film preparation
Аннотация: Cupriavidus eutrophus В10646 was used to synthesize a series of polyhydroxyalkanoate (PHA) quaterpolymers composed of the short-chain-length 3-hydroxybutyrate (3HB), 4-hydroxybutyrate (4HB), and 3-hydroxyvalerate (3HV) and the medium-chain-length 3-hydroxyhexanoate (3HHx). The molar fraction of 3HB in the quaterpolymers varied between 63.5 and 93.1 mol.%, 3HV – between 1.1 and 24.6 mol.%, 4HB – between 2.4 and 15.6 mol.%, and 3HHx – between 0.4 and 4.8 mol.%. The properties of PHA quaterpolymers were significantly different from those of the P(3HB) homopolymer: they had much lower degrees of crystallinity (up to 30–45%), and lower melting points and thermal decomposition temperatures, with the interval between these temperatures remaining practically unchanged. Films prepared from PHA quaterpolymers were rougher and more porous than P(3HB) films; they showed higher values of elongation at break (up to 6–113%), i.e. were more elastic. Films prepared from PHA quaterpolymers were biocompatible and had no toxic effect on mouse fibroblast NIH 3T3 cells. © 2016 Elsevier Ltd

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Держатели документа:
Siberian Federal University, 79 Svobodnyi Avenue, Krasnoyarsk, Russian Federation
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation
Institute of Chemistry and Chemical Technology of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation
L.V. Kirensky Institute of Physics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Volova, T. G.; Vinogradova, O. N.; Zhila, N. O.; Peterson, I. V.; Kiselev, E. G.; Vasiliev, A. D.; Sukovatiy, A. G.; Shishatskaya, E. I.

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20.

Hybrid Minimal Core Streptavidin–Obelin as a Versatile Reporter for Bioluminescence-based Bioassay / E. E. Bashmakova [et al.] // Photochem. Photobiol. - 2017. - Vol. 93, Is. 2. - P548-552, DOI 10.1111/php.12648 . - ISSN 0031-8655
Аннотация: Ca2+-regulated photoprotein obelin was genetically fused with a minimum-sized core streptavidin. Hybrid protein (SAV–OL) was produced by bacterial expression and applied as a specific bioluminescent probe in diverse solid-phase assays. The obtained results clearly demonstrate specific activity of each domain indicating its proper folding with favorable space orientation. SAV–OL has been shown to be a much more sensitive label than the chemical conjugate of a full-length streptavidin with obelin. © 2016 The American Society of Photobiology

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Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Chemistry Department, Lomonosov Moscow State University, Moscow, Russian Federation

Доп.точки доступа:
Bashmakova, E. E.; Krasitskaya, V. V.; Kudryavtsev, A. N.; Grigorenko, V. G.; Frank, L. A.

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