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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Dubinnyi M. A., Ivanov I. A., Rodionova N. S., Kovalchuk S. I., Kaskova Z. M., Petushkov V. N.
Заглавие : α-C-Mannosyltryptophan is a Structural Analog of the Luciferin from Bioluminescent Siberian Earthworm Henlea sp.
Место публикации : ChemistrySelect: Wiley-Blackwell, 2020. - Vol. 5, Is. 42. - С. 13155-13159. - ISSN 23656549 (ISSN), DOI 10.1002/slct.202003075
Аннотация: Cold extract from bioluminescent earthworm Henlea sp. was studied by HPLC, 1D and 2D NMR and LC-HRMS analysis. An abundant structural analog of the luciferin was isolated and identified as ?-C-mannosyltryptophan (ManTrp), the product of unusual C2-glycosylation found earlier in humans, ascidians and other animals. Two compounds in cold extract (P300b, P300c) were characterized as C2-substituted derivatives of tryptophan. We hypothesize that a series of tryptophan-containing compounds are possible participants of bioluminescence-related metabolism in Henlea sp. © 2020 Wiley-VCH GmbH
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Diaz A. V., Ageev, Aleksander Vladimirovich, Ivanova, Elena Anatolevna, Anishchenko, Olesia Valerevna
Заглавие : Winter atmospheric nutrient and pollutant deposition on Western Sayan Mountain lakes (Siberia)
Колич.характеристики :18 с
Коллективы : Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [20-04-00960]; Ministry of Science and Higher Education of the Russian Federation [5-100, FSRZ-2020-0014]
Место публикации : Biogeosciences: COPERNICUS GESELLSCHAFT MBH, 2021. - Vol. 18, Is. 5. - С. 1601-1618. - ISSN 1726-4170, DOI 10.5194/bg-18-1601-2021. - ISSN 1726-4189(eISSN)
Примечания : Cited References:86. - This research has been supported by the Russian Foundation for Basic Research (grant no. 20-04-00960) and the Ministry of Science and Higher Education of the Russian Federation (postdoctoral program project no. 5-100, grant no. FSRZ-2020-0014).
Предметные рубрики: FLY-ASH PARTICLES
NITROGEN DEPOSITION
PHOSPHORUS DEPOSITION
Аннотация: The world map of anthropogenic atmospheric nitrogen deposition and its effects on natural ecosystems is not described with equal precision everywhere. In this paper, we report atmospheric nutrient, sulfate and spheroidal carbonaceous particle (SCP) deposition rates, based on snowpack analyses of a formerly unexplored Siberian mountain region. Then, we discuss their potential effects on lake phytoplankton biomass limitation. We estimate that the nutrient depositions observed in the late-season snowpack (40 +/- 16 mgNO(3)-Nm(-2) and 0.58 +/- 0.13 mg TP-Pm-2; TP for total phosphorous) would correspond to yearly depositions lower than 119 +/- 71 mgNO(3)-Nm(-2) yr(-1) and higher than 1.71 +/- 0.91 mg TP-Pm-2 yr(-1). These yearly deposition estimates would approximately fit the predictions of global deposition models and correspond to the very low nutrient deposition range, although they are still higher than world background values. In spite of the fact that such a low atmospheric nitrogen deposition rate would be enough to induce nitrogen limitation in unproductive mountain lakes, phosphorus deposition was also extremely low, and the resulting lake water N: P ratio was unaffected by atmospheric nutrient deposition. In the end, the studied lakes' phytoplankton appeared to be split between phosphorus and nitrogen limitation. We conclude that these pristine lakes are fragile sensitive systems exposed to the predicted climate warming, increased winter precipitation, enhanced forest fires and shifts in anthropogenic nitrogen emissions that could finally couple their water chemistry to that of atmospheric nutrient deposition and unlock temperature-inhibited responses of phytoplankton to nutrient shifts.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Diaz-De-Quijano D., Vladimirovich Ageev A., Anatolevna Ivanova E., Valerevna Anishchenko O.
Заглавие : Winter atmospheric nutrient and pollutant deposition on Western Sayan Mountain lakes (Siberia)
Место публикации : Biogeosciences: Copernicus GmbH, 2021. - Vol. 18, Is. 5. - С. 1601-1618. - ISSN 17264170 (ISSN), DOI 10.5194/bg-18-1601-2021
Аннотация: The world map of anthropogenic atmospheric nitrogen deposition and its effects on natural ecosystems is not described with equal precision everywhere. In this paper, we report atmospheric nutrient, sulfate and spheroidal carbonaceous particle (SCP) deposition rates, based on snowpack analyses of a formerly unexplored Siberian mountain region. Then, we discuss their potential effects on lake phytoplankton biomass limitation. We estimate that the nutrient depositions observed in the late-season snowpack (4016 mgNO3-Nm2 and 0.580.13 mg TP-Pm2; TP for total phosphorous) would correspond to yearly depositions lower than 11971 mgNO3-Nm2 yr1 and higher than 1.710.91 mg TP-Pm2 yr1. These yearly deposition estimates would approximately fit the predictions of global deposition models and correspond to the very low nutrient deposition range, although they are still higher than world background values. In spite of the fact that such a low atmospheric nitrogen deposition rate would be enough to induce nitrogen limitation in unproductive mountain lakes, phosphorus deposition was also extremely low, and the resulting lake water N: P ratio was unaffected by atmospheric nutrient deposition. In the end, the studied lakes' phytoplankton appeared to be split between phosphorus and nitrogen limitation. We conclude that these pristine lakes are fragile sensitive systems exposed to the predicted climate warming, increased winter precipitation, enhanced forest fires and shifts in anthropogenic nitrogen emissions that could finally couple their water chemistry to that of atmospheric nutrient deposition and unlock temperature-inhibited responses of phytoplankton to nutrient shifts. © Author(s) 2021. This work is distributed under the Creative Commons Attribution 4.0 License.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Frank L.A., Borisova V.V., Markova S.V., Malikova N.P., Stepanyuk G.A., Vysotski E.S.
Заглавие : Violet and greenish photoprotein obelin mutants for reporter applications in dual-color assay
Колич.характеристики :6 с
Место публикации : Anal. Bioanal. Chem.: SPRINGER HEIDELBERG, 2008. - Vol. 391, Is. 8. - С. 2891-2896. - ISSN 1618-2642, DOI 10.1007/s00216-008-2223-5
Примечания : Cited References: 22
Предметные рубрики: ANGSTROM RESOLUTION
RECOMBINANT OBELIN
CRYSTAL-STRUCTURE
BIOLUMINESCENCE
AEQUORIN
IMMUNOASSAY
EXPRESSION
CDNA
PURIFICATION
CLONING
Ключевые слова (''Своб.индексиров.''): ca(2+)-regulated photoprotein--bioluminescence--dual-color assay
Аннотация: Two kinds of Ca(2+)-regulated photoprotein obelin with altered color of bioluminescence were obtained by active-center amino acid substitution. The mutant W92F-H22E emits violet light (lambda(max)=390 nm) and the mutant Y139F emits greenish light (lambda (max)=498 nm), with small spectral overlap, both display high activity and stability and thus may be used as reporters. For demonstration, the mutants were applied in dual-color simultaneous immunoassay of two gonadotropic hormones-follicle-stimulating hormone and luteinizing hormone. Bioluminescence of the reporters was simultaneously triggered by single injection of Ca(2+) solution, divided using band-pass optical filters and measured with a two-channel photometer. The sensitivity of simultaneous bioluminescence assay was close to that of a separate radioimmunoassay.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zotina T. A., Trofimova E. A., Medvedeva M. Y., Dementyev D. V., Bolsunovsky A. Y.
Заглавие : Use of the aquatic plant Elodea canadensis to assess toxicity and genotoxicity of Yenisei River sediments
Место публикации : Environ. Toxicol. Chem. - 2015. - Vol. 34, Is. 10. - С. 2310-2321. - ISSN 07307268 (ISSN) , DOI 10.1002/etc.3057
Ключевые слова (''Своб.индексиров.''): aquatic plants--biomarkers--genotoxicity--sediment quality--sediment toxicity--biomarkers--chromosomes--cytotoxicity--pollution--pollution control--radioactive waste disposal--radioactivity--river pollution--sediments--toxicity--aquatic plants--genotoxicities--laboratory bioassay--radioactive contamination--radioactive pollution--sediment quality--sediment toxicity--toxicity endpoints--rivers--article--bioassay--controlled study--cytotoxicity--elodea canadensis--environmental exposure--genotoxicity--indicator organism--lake sediment--mitosis index--nonhuman--plant growth--plant root--priority journal--radioactive pollution--river--root length--russian federation--sensitivity analysis--shoot--toxicity testing--elodea--elodea canadensis
Аннотация: The toxicity, cytotoxicity, and genotoxicity of bulk sediments from the Yenisei River (Siberia, Russia) were estimated in laboratory bioassays based on several endpoints in the aquatic plant Elodea canadensis. The bottom sediment samples were collected in the Yenisei River upstream and downstream of the sources of chemical and radioactive contamination. The testing revealed different sensitivities of Elodea endpoints to the quality of the bottom sediment: weight of shootslength of shootsmitotic indexlength of rootspercentage of abnormal cells. The response of the genotoxicity endpoint (percentage of cells with chromosome abnormalities in roots of Elodea) was the highest in sediments with chemical pollution, whereas the highest inhibition of toxicity endpoints (shoot and root length) occurred in sediments with the highest level of radioactive pollution. The extreme response of Elodea endpoints to the quality of certain sediment samples may be regarded as related to the possible presence of unknown toxicants. The results show that E. canadensis can be used as an indicator species in laboratory contact testing of bottom sediment. The responses of shoot and root length growth endpoints of Elodea can be recommended as basic sensitivity indicators of bottom sediment toxicity. Analysis of cells carrying abnormal chromosomes in the apical root meristem of Elodea can be performed optionally in the same test to assess the genotoxicity of sediments. © 2015 SETAC.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Van Stokkum I.H.M., Gobets B., Van Mourik F., Lee J., Van Grondelle R., Visser A.J.W.G.
Заглавие : Ultrafast fluorescence relaxation spectroscopy of 6,7-dimethyl-(8-ribityl)-lumazine and riboflavin, free and bound to antenna proteins from bioluminescent bacteria
Место публикации : Journal of Physical Chemistry B. - 2003. - Vol. 107, Is. 39. - С. 10934-10939. - ISSN 15206106 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacteria--bioluminescence--chemical relaxation--chromophores--dielectric properties--proteins--solvents--bioluminescent bacteria--dimethyl ribityl lumazine--photobacterium leiognathi--riboflavin--ultrafast fluorescence relaxation spectroscopy--fluorescence
Аннотация: The solvation dynamics of interesting bioluminescent chromophores have been determined, using subpicosecond and wavelength-resolved fluorescence spectroscopy, in combination with global analysis of the multidimensional data sets. The systems investigated comprise the free ligands 6,7-dimethyl-(8-ribityl)-lumazine (lumazine) and riboflavin in an aqueous buffer and both ligands when noncovalently bound to two bacterial bioluminescent antenna proteins: lumazine protein (from Photobacterium leiognathi) and the blue fluorescent protein (from Vibrio fischeri Y1). Fluorescence spectral relaxation of the free ligands is complete within a few picoseconds. Subsequently, the fluorescence intensity increases by ?7% on a time scale of 15-30 ps. Fluorescence spectral relaxation of the protein-bound ligands is largely complete within 1 ps but reveals a small red shift with a minor, but distinctly longer, relaxation time than that of the free ligands, which is tentatively assigned to the relaxation of protein-bound water in the vicinity of the excited chromophore.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., van Stokkum IHM, Gobets B..., van Mourik F..., Lee J..., van Grondelle R..., Visser AJWG
Заглавие : Ultrafast fluorescence relaxation spectroscopy of 6,7-dimethyl-(8-ribityl)-lumazine and riboflavin, free and bound to antenna proteins from bioluminescent bacteria
Колич.характеристики :6 с
Место публикации : J. Phys. Chem. B: AMER CHEMICAL SOC, 2003. - Vol. 107, Is. 39. - P10934-10939. - ISSN 1520-6106, DOI 10.1021/jp034266e
Примечания : Cited References: 52
Предметные рубрики: TIME-RESOLVED FLUORESCENCE
VIBRIO-FISCHERI Y1
FEMTOSECOND SOLVATION DYNAMICS
FLAVIN ADENINE-DINUCLEOTIDE
PHOTOBACTERIUM-LEIOGNATHI
BIOLOGICAL WATER
SOLVENT DYNAMICS
DIELECTRIC-RELAXATION
MOLECULAR-DYNAMICS
TRYPTOPHAN
Аннотация: The solvation dynamics of interesting bioluminescent chromophores have been determined, using subpicosecond and wavelength-resolved fluorescence spectroscopy, in combination with global analysis of the multidimensional data sets. The systems investigated comprise the free ligands 6,7-dimethyl-(8-ribityl)-lumazine (lumazine) and riboflavin in an aqueous buffer and both ligands when noncovalently bound to two bacterial bioluminescent antenna proteins: lumazine protein (from Photobacterium leiognathi) and the blue fluorescent protein (from Vibrio fischeri Y1). Fluorescence spectral relaxation of the free ligands is complete within a few picoseconds. Subsequently, the fluorescence intensity increases by similar to7% on a time scale of 15-30 ps. Fluorescence spectral relaxation of the protein-bound ligands is largely complete within 1 ps but reveals a small red shift with a minor, but distinctly longer, relaxation time than that of the free ligands, which is tentatively assigned to the relaxation of protein-bound water in the vicinity of the excited chromophore.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M. I., Artamonova V. S., Makhrov A. A., Sushchik N. N., Kalachova G. S., Dgebuadze Y. Y.
Заглавие : Triploidy does not decrease contents of eicosapentaenoic and docosahexaenoic acids in filets of pink salmon Oncorhynchus gorbuscha
Место публикации : Food Chem. - 2017. - Vol. 216. - С. 66-69. - ISSN 03088146 (ISSN) , DOI 10.1016/j.foodchem.2016.08.021
Ключевые слова (''Своб.индексиров.''): essential fatty acids--filets--triploid fish--aquaculture--fish--polyunsaturated fatty acids--unsaturated fatty acids--docosahexaenoic acid--eicosapentaenoic acid--essential fatty acids--fa compositions--filets--muscle tissues--pink salmon--white sea--fatty acids
Аннотация: Triploid fish has become an important item of commercial aquaculture, but data on its fatty acid (FA) composition are still controversial, especially regarding essential polyunsaturated fatty acids, eicosapentaenoic acid (20:5n-3, EPA) and docosahexaenoic acid (22:6n-3, DHA). We studied FA composition and content of diploid and triploid pink salmon Oncorhynchus gorbuscha, reared in aquaculture in a bay of the White Sea (Russia). FA composition, measured as percentages of total FA of triploids and immature diploid females significantly differed from that of mature diploid fish. Specifically, mature diploids had higher percentage of EPA and DHA in their muscle tissue (filets) compared to that of triploids and immature diploid females. Nevertheless, the contents of EPA and DHA per mass of the filets in diploid and triploid specimens were similar. Thus, no special efforts are needed to improve EPA and DHA contents in filets of triploids. © 2016 Elsevier Ltd
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Krylov A.V., Sushchik N.N., Malin M.I., Makhutova O.N., Chalova I.V., Kalacheva G.S.
Заглавие : Transfer of essential polyunsaturated fatty acids from an aquatic to terrestrial ecosystem through the fish-bird trophic pair
Место публикации : Doklady Biological Sciences. - 2010. - Vol. 431, Is. 1. - С. 121-123. - ISSN 00124966 (ISSN) , DOI 10.1134/S0012496610020146
Ключевые слова (''Своб.индексиров.''): fatty acid--fresh water--triacylglycerol--unsaturated fatty acid--animal--article--biological model--biomass--bird--chemistry--ecosystem--fish--food chain--metabolism--muscle--pathology--physiology--species difference--animals--biomass--birds--ecosystem--fatty acids--fatty acids, unsaturated--fishes--food chain--fresh water--models, biological--muscles--species specificity--triglycerides--aves
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Efremov S.N., Puzyr' A.P., Mogil'Naya O.A.
Заглавие : Tissue response to biodegradable suture threads made of polyhydroxyalkanoates
Место публикации : Biomedical Engineering. - 2002. - Vol. 36, Is. 4. - С. 210-217. - ISSN 00063398 (ISSN) , DOI 10.1023/A:1021184119268
Ключевые слова (''Своб.индексиров.''): acid phosphatase--alkaline phosphatase--polyhydroxyalkanoic acid--animal experiment--animal tissue--article--biocompatibility--biodegradability--controlled study--elasticity--enzyme activity--enzyme mechanism--female--histochemistry--incision--nonhuman--physical chemistry--postoperative period--rat--rigidity--suture--thickness--tissue reaction--wound healing--animalia
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Rogozin D.Yu., Pimenov N.V., Kosolapov D.B., Chan'kovskaya Yu.V., Degermendzhy A.G.
Заглавие : Thin-layer vertical distribution of purple sulfur bacteria in the chemoclines of meromictic lakes Shira and Shunet (Khakasia, Southern Siberia)
Место публикации : Doklady Akademii Nauk. - 2005. - Vol. 400, Is. 3. - С. 426-429. - ISSN 08695652 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacteria--bathymetry--biochemistry--geochemistry--physical chemistry--water bacteriology--bathometers--vertical distribution--lakes
Аннотация: Vertical inhomogeneity of microbial population, biogeochemical processes and physicochemical characteristics of chemocline was revealed using specially created stratification bathometer (sampler) consisting of syringes horizontally placed one over another on a carrying frame.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S.
Заглавие : The synthesis of hydroxybutyrate and hydroxyvalerate copolymers by the bacterium Ralstonia eutropha
Место публикации : Mikrobiologiya. - 2005. - Vol. 74, Is. 1. - С. 63-69. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): ?-ketothiolase--controlled synthesis--poly(hydroxybutyrate-co-hydroxyvalerate)--ralstonia eutropha--bacteria (microorganisms)--cupriavidus necator--acetoacetyl coenzyme a--acetyl coenzyme a acyltransferase--acyl coenzyme a--acyltransferase--butyric acid derivative--carbon dioxide--fructose--hydrogen--poly(3 hydroxybutyrate) co (3 hydroxyvalerate)--poly(3-hydroxyalkanoic acid) synthase--poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate)--polyester--polyhydroxyalkanoate synthase--valeric acid derivative--article--chemistry--crystallization--culture medium--metabolism--wautersia eutropha--acetyl-coa c-acyltransferase--acyl coenzyme a--acyltransferases--butyrates--carbon dioxide--crystallization--culture media--cupriavidus necator--fructose--hydrogen--polyesters--valerates
Аннотация: The paper deals with the study of the synthesis of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) copolymers by the bacterium Ralstonia eutropha B-5786 grown under different carbon nutrition conditions (growth on carbon dioxide, fructose, and CO2-valerate and fructose-valerate mixtures). The parameters to be analyzed included the yield of biomass, the yield, synthesis rate, and composition of copolymers, the activity of the key enzymes of polyhydroxyalkanoate (PHA) synthesis (?-ketothiolase, acetoacetyl-CoA reductase, and PHA synthase), the maximum tolerable concentration of valerate to the bacterium, and the conditions that govern the incorporation of hydroxyvalerate to copolymers. This allowed the relationship between cultivation conditions and the proportion of monomers in the copolymers to be deduced. We were able to synthesize a range of 3HB/3HV copolymers and found that the thermal characteristics and the degree of crystallinity of these copolymers depend on the molar fraction of 3HV.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Karpowicz M., Feniova I., Gladyshev M. I., Ejsmont-Karabin J., Gorniak A., Zielinski P., Dawidowicz P., Kolmakova A. A., Dzialowski A. R.
Заглавие : The stoichiometric ratios (C:N:P) in a pelagic food web under experimental conditions
Место публикации : Limnologica: Elsevier GmbH, 2019. - Vol. 77. - Ст.125690. - ISSN 00759511 (ISSN) , DOI 10.1016/j.limno.2019.125690
Аннотация: Interactions between phytoplankton and zooplankton affect the overall functioning of lakes. Herbivores are habitually confronted with food of inferior quality, usually a result of low nutrient concentrations in plant material. Large-bodied cladocerans are better competitors for food than small-bodied species but they are more vulnerable to low food quality. Understanding the effects of food quality on zooplankton structure and competition between small - large bodied herbivorous is of considerable interest. We want to find out how differences in C:N:P ratios between phytoplankton and zooplankton communities affect their abundances in a freshwater food web. We want also to assess the role of phytoplankton and zooplankton as sinks of the phosphorus and nitrogen. Therefore, we conducted a 31-day mesocosms experiment with water from a mesotrophic and a eutrophic lake (with natural plankton communities). To simulate changes in the plankton communities large-bodied Daphnia magna and Daphnia pulicaria were added. Samples for zooplankton, phytoplankton and water chemistry were taken every 10 days. Samples for elemental analysis (C:N:P) of seston and zooplankton were collected on the first, and on the final day of the experiment. Our mesocosms experiment showed mismatch in C:P between seston (high) and zooplankton (low), which suggests that most of the phosphorus is incorporated in zooplankton biomass. This evidenced that zooplankton is an effective sink of phosphorus, while nitrogen is accumulated mainly by primary producers. Our results also indicated more stability in stoichiometry with increasing trophic levels of organisms. However, there were significant changes in the zooplankton structure. The increasing dominance of large Daphnia resulted in reduction of C:P ratio in zooplankton. Low food quality (C:P) did not limit the growth of large Daphnia in the experimental conditions, which competed effectively with small planktonic cladocerans and with Rotifera. Over time, inedible algae began to dominate resulting in increase of relative biomass of periphyton grazers, which suggests that plankton community is transformed into littoral system in mesocosms for about 30 days. © 2019
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Belyaev B.A., Volova T.G., Drokin N.A., Shepov V.N.
Заглавие : The SHF dielectric permeability of polyhydroxybutyrate, a degradable biological polymer.
Место публикации : Doklady Biophysics. - 2000. - Vol. 370-372. - С. 9-12. - ISSN 00124974 (ISSN)
Ключевые слова (''Своб.индексиров.''): hydroxybutyric acid--polymer--article--chemistry--electricity--electromagnetic field--temperature--electromagnetic fields--electrostatics--hydroxybutyrates--polymers--temperature
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gorokhovatsky A. Y., Chepurnykh T. V., Shcheglov A. S., Mokrushina Y. A., Baranova M. N., Goncharuk S. A., Purtov K. V., Petushkov V. N., Rodionova N. S., Yampolsky I. V.
Заглавие : The Recombinant Luciferase of the Fungus Neonothopanus nambi: Obtaining and Properties
Место публикации : Doklad. Biochem. Biophys.: Pleiades journals, 2021. - Vol. 496, Is. 1. - С. 52-55. - ISSN 16076729 (ISSN), DOI 10.1134/S1607672921010051
Аннотация: Abstract: A key component of the recently described bioluminescent system of higher fungi is luciferase, a new class of proteins. The properties of fungal luciferase and their relationship with its structure are interesting both for improving autoluminescent systems already created on its basis and for creating new ones. Therefore, it is extremely important to understand the spatial structure of this protein. We have performed heterologous expression and purification of Neonothopanus nambi luciferase, obtained a protein suitable for subsequent crystallization, and also determined some biochemical properties of the recombinant luciferase. © 2021, The Author(s),.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Voinova O.N., Kalacheva G.S., Grodnitskaya I.D.
Заглавие : The prospects of the use of resorbable polyesters for designing safe pesticides
Место публикации : Doklady Biological Sciences. - 2008. - Vol. 419, Is. 1. - С. 100-103. - ISSN 00124966 (ISSN) , DOI 10.1134/S0012496608020099
Ключевые слова (''Своб.индексиров.''): pesticide--polyester--article--chemistry--drug design--metabolism--microbiology--drug design--pesticides--polyesters--soil microbiology
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18.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Eremeeva E.V., Markova S.V., Frank L.A., Vysotski E.S.
Заглавие : THE MAIN FUNCTION OF HIS175, TRP179, AND TYR190 RESIDUES OF THE OBELIN BINDING SITE IS TO STABILIZE THE HYDROPEROXYCOELENTERAZINE INTERMEDIATE
Колич.характеристики :4 с
Место публикации : BIOLUMINESCENCE AND CHEMILUMINESCENCE: CHEMISTRY, BIOLOGY AND APPLICATIONS: WORLD SCIENTIFIC PUBL CO PTE LTD, 2007. - 14th International Symposium on Bioluminescence and Chemiluminescence (OCT 15-19, 2006, San Diego, CA). - С. 7-10. - ISBN 978-981-270-816-8, DOI 10.1142/9789812770196_0002
Примечания : Cited References: 7
Предметные рубрики: PHOTOPROTEIN OBELIN
BIOLUMINESCENCE
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondar V.S., Puzyr A.P., Purtov K.V., Medvedeva S.E., Rodicheva E.K., Gitelson J.I.
Заглавие : The luminescent system of the luminous fungus Neonothopanus nambi
Место публикации : Doklady Biochemistry and Biophysics. - 2011. - Vol. 438, Is. 1. - С. 138-140. - ISSN 16076729 (ISSN) , DOI 10.1134/S1607672911030082
Ключевые слова (''Своб.индексиров.''): agaricales--article--chemistry--luminescence--agaricales--luminescence--fungi--neonothopanus nambi
Scopus
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Burakova L. P., Eremeeva E. V., Vysotski E. S.
Заглавие : The interaction of C-terminal Tyr208 and Tyr13 of the first α-helix ensures a closed conformation of ctenophore photoprotein berovin
Место публикации : Photochem. Photobiol. Sci. - 2020. - Vol. 19, Is. 3. - С. 313-323. - ISSN 1474905X (ISSN), DOI 10.1039/c9pp00436j
Аннотация: Light-sensitive Ca2+-regulated photoprotein berovin is responsible for the bioluminescence of the ctenophore Beroe abyssicola. It shares many properties of hydromedusan photoproteins although the degree of identity of its amino acid sequence with those of photoproteins is low. There is a hydrogen bond between C-terminal Pro and Arg situated in the N-terminal ?-helix of hydromedusan photoproteins that supports a closed conformation of the internal cavity of the photoprotein molecule with bound 2-hydroperoxycoelenterazine. The C- and N-terminal hydrogen bond network is necessary to properly isolate the photoprotein active site from the solvent and consequently to provide a high quantum yield of the bioluminescence reaction. In order to find out which berovin residues perform the same function we modified the N- and C-termini of the protein by replacing or deleting various amino acid residues. The studies on berovin mutants showed that the interaction between C-terminal Tyr208 and Tyr13 localized in the first ?-helix of the photoprotein is important for the stabilization and proper orientation of the oxygenated coelenterazine adduct within the internal cavity as well as for supporting the closed photoprotein conformation. We also suggest that the interplay between Tyr residues in ctenophore photoproteins occurs rather through the ?-? interaction of their phenyl rings than through hydrogen bonds as in hydromedusan photoproteins. This journal is © The Royal Society of Chemistry and Owner Societies.
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