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Gene pool maintenance and the prospects for using a special-purpose luminous bacteria collection / E. K. Rodicheva [et al.] // Applied Biochemistry and Microbiology. - 1998. - Vol. 34, Is. 1. - P70-76 . - ISSN 0003-6838
Аннотация: The culture collection maintained at the Institute of Biophysics (IB), Siberian Division, Russian Academy of Sciences, is the only deposit in Russia and CIS which contains about 700 strains of five species of marine luminous bacteria sampled in various sites of the World Ocean. The strains collected are used for identification of luminous bacteria; studying their physiology, biochemistry, and cytology; and developing concepts of organization and metabolism of luminous bacteria under natural and laboratory conditions. Methods of their storage and practical use are developed. The results of these studies are summarized in a data bank, which will be incorporated into the general network of data banks on microbial cultures kept in Russia and into the international network of data banks on microorganisms (MSDN). Systematization of information on luminous bacteria possessing a unique visual marker allows their extensive use in basic research and genetic engineering, for environmental and medical applications, and for biotechnological purposes.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Rodicheva, E.K.; Medvedeva, S.E.; Vydryakova, G.A.; Chugaeva, Yu.V.; Kuznetsov, A.M.

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Cytotoxicity of polyhydroxyalkanoates in animal cell cultures. / E. I. Shishatskaya [et al.] // Doklady Biological Sciences. - 2000. - Vol. 374, Is. 1-6. - P539-542 . - ISSN 0012-4966
Кл.слова (ненормированные):
DNA -- hydroxybutyric acid -- poly(3 hydroxybutyrate) co (3 hydroxyvalerate) -- poly(3 hydroxybutyric acid) -- poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate) -- poly-beta-hydroxybutyrate -- polyester -- RNA -- thymidine -- uridine -- animal -- article -- biosynthesis -- cell division -- cell strain 3T3 -- cell survival -- cytology -- drug effect -- liver -- liver cell -- metabolism -- mouse -- vascular endothelium -- 3T3 Cells -- Animals -- Cell Division -- Cell Survival -- DNA -- Endothelium, Vascular -- Hepatocytes -- Hydroxybutyrates -- Liver -- Mice -- Polyesters -- RNA -- Thymidine -- Uridine

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Eremeev, A.V.; Gitel'zon, I.I.; Setkov, N.A.; Volova, T.G.

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DNA synthesis in heterokaryons obtained by fusion of hepatocytes from a regenerating mouse liver and NIH 3T3 fibroblasts / A. V. Eremeev, N. A. Setkov // Doklady Biological Sciences. - 2000. - Vol. 372, Is. 1-6. - P. 329-332 . - ISSN 0012-4966
Кл.слова (ненормированные):
DNA -- thymidine -- animal -- article -- biosynthesis -- cell division -- cell fusion -- cell strain 3T3 -- cytology -- hybrid cell -- kinetics -- liver cell -- liver regeneration -- metabolism -- mouse -- 3T3 Cells -- Animals -- Cell Division -- Cell Fusion -- DNA -- Hepatocytes -- Hybrid Cells -- Kinetics -- Liver Regeneration -- Mice -- Thymidine

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Eremeev, A.V.; Setkov, N.A.

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Secretion of free fatty acids by prokaryotic and eukaryotic algae at optimal, supraoptimal, and suboptimal growth temperatures / N. N. Sushchik, G. S. Kalacheva, M. I. Gladyshev // Mikrobiologiya. - 2001. - Vol. 70, Is. 5. - С. 629-635 . - ISSN 0026-3656
Кл.слова (ненормированные):
Free fatty acids -- Microalgae -- Secretion -- fatty acid -- article -- chemistry -- Chlorella -- Cyanobacterium -- cytology -- extracellular space -- growth, development and aging -- intracellular fluid -- temperature -- Chlorella -- Cyanobacteria -- English Abstract -- Extracellular Space -- Fatty Acids, Nonesterified -- Intracellular Fluid -- Temperature
Аннотация: Abstract-The paper describes the composition of extracellular free fatty acids (FFAs) and intracellular fatty acids (FAs) in the enrichment cultures of the prokaryotic alga Spirulina platensis and the eukaryotic alga Chlorella vulgaris grown at optimal, supraoptimal, and suboptimal growth temperatures. With increasing growth temperature, the degree of unsaturation of the intracellular FAs of both algae decreased, while that of the extracellular FFAs of 5. platensis increased. The composition of the extracellular FFAs of C. vulgaris practically did not depend on the growth temperature.

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Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sushchik, N.N.; Kalacheva, G.S.; Gladyshev, M.I.

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Experimental study of postwintering germination of akinetes and dormant cells of cyanoprocaryotes from bottom sediments of blooming and nonblooming ponds. / M. I. Gladyshev [et al.] // Doklady Biological Sciences. - 2002. - Vol. 383, Is. 1-6. - P131-132 . - ISSN 0012-4966
Кл.слова (ненормированные):
fresh water -- article -- Cyanobacterium -- cytology -- growth, development and aging -- microbiology -- physiology -- season -- Cyanobacteria -- Fresh Water -- Seasons -- Water Microbiology

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gladyshev, M.I.; Kravchuk, E.S.; Kolmakov, V.I.; Ivanova, E.A.; Trusova, M.Y.; Sushchik, N.N.; Gribovskaya, I.V.

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In vivo toxicological evaluation of polyhydroxyalkanoates. / E. I. Shishatskaya, T. G. Volova, I. I. Gitelson // Doklady Biological Sciences. - 2002. - Vol. 383, Is. 1-6. - P109-111 . - ISSN 0012-4966
Кл.слова (ненормированные):
alkane -- animal -- animal behavior -- article -- cytology -- drug effect -- erythrocyte sedimentation rate -- female -- heart -- kidney -- liver -- lymphoid tissue -- male -- rat -- spleen -- Wistar rat -- Alkanes -- Animals -- Behavior, Animal -- Blood Sedimentation -- Female -- Heart -- Kidney -- Liver -- Lymphoid Tissue -- Male -- Rats -- Rats, Wistar -- Spleen

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Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Gitelson, I.I.

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Self-restoration as fundamental property of CES providing their sustainability / I. I. Gitelson, A. G. Degermendzhy, E. K. Rodicheva // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1641-1648, DOI 10.1016/S0273-1177(03)00111-X . - ISSN 0273-1177
Кл.слова (ненормированные):
Biocatalysts -- Ecosystems -- Genes -- Life support systems (spacecraft) -- Radiation damage -- Cell populations -- Space flight -- space shuttle -- article -- biological model -- biomass -- cell division -- Chlorella -- cytology -- growth, development and aging -- microclimate -- radiation exposure -- radiation response -- ultraviolet radiation -- Biomass -- Cell Division -- Chlorella -- Dose-Response Relationship, Radiation -- Ecological Systems, Closed -- Life Support Systems -- Models, Biological -- Ultraviolet Rays
Аннотация: Sustainability is one of the most important criteria and evaluation of human life support systems intended for use during long space flights. The common feature of biological and physicochemical life support systems is that basically they are both catalytic. But there are two fundamental properties distinguishing biological systems: 1) they are auto-catalytic: their catalysts - enzymes of protein nature - are continuously reproduced when the system functions; 2) the program of every process performed by enzymes and the program of their reproduction are inherent in the biological system itself - in the totality of genomes of the species involved in the functioning of the ecosystem. Actually, one cell with the genome capable of the phenotypic realization is enough for the self-restoration of the function performed by the cells of this species in the ecosystem. The continuous microalgal culture of Chlorella vulgaris was taken to investigate quantitatively the process of self-restoration in unicellular algae population. Based on the data obtained, we proposed a mathematical model of the restoration process in a cell population that has suffered an acute radiation damage. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, SB RAS, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, I.I.; Degermendzhy, A.G.; Rodicheva, E.K.

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A hybrid PHB-hydroxyapatite composite for biomedical application: Production, in vitro and in vivo investigation / E. I. Shishatskaya, I. A. Khlusov, T. G. Volova // Journal of Biomaterials Science, Polymer Edition. - 2006. - Vol. 17, Is. 5. - P481-498, DOI 10.1163/156856206776986242 . - ISSN 0920-5063
Кл.слова (ненормированные):
Biocompatibility -- Hydroxyapatite (HA) -- PHB-hydroxyapatite composite -- Polyhydroxyalkanoate (PHA) -- Polyhydroxybutyrate (P(3HB)) -- Properties -- Biocompatibility -- Differential thermal analysis -- Electron microscopy -- Free energy -- Interfacial energy -- Physical properties -- Surface properties -- X ray analysis -- Biomedical application -- Physicochemical properties -- Polyhydroxyalkanoate (PHA) -- Polyhydroxybutyrate (PHB) -- Hydroxyapatite -- hydroxyapatite -- poly(3 hydroxybutyric acid) -- polymer -- biomaterial -- hydroxybutyric acid -- adhesion -- animal cell -- animal tissue -- article -- biomedicine -- bone marrow cell -- cell differentiation -- cell growth -- chemical structure -- composite material -- controlled study -- crystallization -- decomposition -- electron microscopy -- in vitro study -- in vivo study -- melting point -- mouse -- nonhuman -- ossification -- osteoblast -- physical chemistry -- priority journal -- rat -- strength -- structure analysis -- surface property -- synthesis -- temperature measurement -- thermal analysis -- tissue engineering -- wettability -- animal -- biomechanics -- bioremediation -- bone prosthesis -- cattle -- cell culture -- chemistry -- cytology -- differential scanning calorimetry -- drug effect -- human -- materials testing -- prostheses and orthoses -- scanning electron microscopy -- standard -- Wistar rat -- Murinae -- Animals -- Biocompatible Materials -- Biodegradation, Environmental -- Biomechanics -- Bone Substitutes -- Cattle -- Cells, Cultured -- Differential Thermal Analysis -- Durapatite -- Humans -- Hydroxybutyrates -- Materials Testing -- Microscopy, Electron, Scanning -- Osteoblasts -- Prostheses and Implants -- Rats -- Rats, Wistar -- Surface Properties
Аннотация: Samples of a hybrid composite of polyhydroxybutyrate (PHB), a biodegradable polyester, and hydroxyapatite (HA), with different PHB/HA ratios, have been prepared using mechanical-physical method. Electron microscopy, X-ray structure analysis and differential thermal analysis have been used to investigate the structure and physicochemical properties of the composite, depending on the PHB/HA ratio. The properties of the surface of the HA-loaded composite are significantly different from those of the pure polymer. As the HA percentage in the composite increases, free interface energy, the cohesive force, i.e., the strength of the adhesive bond between the composite surface and the water phase, and surface wettability increase. The HA percentage of the composite does not influence its melting temperature, but affects the temperature for the onset of decomposition: as the HA content increases from 0 to 10% (w/w), Td decreases from 260В°C to 225В°C. The degree of crystallinity of PHB/HA increases from 77% to 89% with an increase in the HA fraction from 10% to 50%. Functional properties of the composites have been investigated in vitro and in vivo. The best parameters of growth and differentiation of murine marrow osteoblasts are registered on PHB/HA samples containing 10% and 20% HA. In ectopic bone formation assay it has been proven that the hybrid PHB/HA composites can function as scaffolds and that bone tissue develops on their surface and in pores. В© VSP 2006.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 60036, Russian Federation
Tomsk State University, Tomsk 634021, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Khlusov, I.A.; Volova, T.G.

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Host-parasite interactions in closed and open microbial cultivation system / T. I. Pisman, N. S. Pechurkin // Advances in Space Research. - 2008. - Vol. 41, Is. 5. - P773-776, DOI 10.1016/j.asr.2007.03.076 . - ISSN 0273-1177
Кл.слова (ненормированные):
Bacterium -- Coevolution -- Continuous and batch culture -- Phage -- Cultivation -- Cytology -- Nutrients -- Soils -- Continuous and batch culture -- Parasite interactions -- Bacteriophages
Аннотация: The study addresses interaction of bacteria and phages in the host-parasite system in batch and continuous cultures. The study system consists of the auxotrophic strain of Brevibacterium - Brevibacterium sp. 22L - and the bacteriophage of Brevibacterium sp., isolated from the soil by the enrichment method.1.Closed system. In the investigation of the relationship between the time of bacterial lysis and multiplicity of phage infection it has been found that at a lower phage amount per cell it takes a longer time for the lysis of the culture to become discernible. Another important factor determining cytolysis in liquid medium is the physiological state of bacterial population. Specific growth rate of bacteria at the moment of phage infection has been chosen as an indicator of the physiological state of bacteria. It has been shown that the shortest latent period and the largest output of the phage are observed during the logarithmic growth phase of bacteria grown under favorable nutrient conditions. In the stationary phase, bacterial cells become "a bad host" for the phage, whose reproduction rate decreases, and the lysis either slows down significantly or does not occur at all.2.Open system. It has been found that in continuous culture, the components of the host-parasite system can coexist over a long period of time. After phage infection, the sizes of the both populations vary for some time and then the density of the host population reaches the level close to that of the uninfected culture. The phage population copies the variations in the density of the host population, but in antiphase. It has been proven that the bacterium becomes resistant to the phage rather soon. It has been supposed that primary resistance is of physiological origin, because the percentage of cells that have survived lysis - about 0.2% of the initial bacterial population - is too high for phage-resistant mutants. Bacteria and phages cultured over extended periods of time in the host-parasite system have been found to co-evolve. The stressful effect of the phage causes development of bacteria resistant to this phage, then a mutant phage capable of lysing these bacteria evolves, etc. В© 2007 COSPAR.

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Держатели документа:
Institute of Biophysics SB RAS, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pisman, T.I.; Pechurkin, N.S.

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10.

Differences in fatty acid composition between smolts of Sockeye Salmon and adults migrated from sea to lake for spawning / M. I. Gladyshev [et al.] // Doklady Biochemistry and Biophysics. - 2010. - Vol. 430, Is. 1. - P29-32, DOI 10.1134/S1607672910010096 . - ISSN 1607-6729
Кл.слова (ненормированные):
fatty acid -- fresh water -- sea water -- animal -- article -- chemistry -- cytology -- female -- male -- metabolism -- muscle -- physiology -- population migration -- reproduction -- salmon -- sea -- Animal Migration -- Animals -- Fatty Acids -- Female -- Fresh Water -- Male -- Muscles -- Oceans and Seas -- Reproduction -- Salmon -- Seawater -- Oncorhynchus nerka

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Kamchatka Research Institute of Fisheries and Oceanography, Naberezhnaya 18, Petropavlovsk-Kamchatskii, 683602, Russian Federation
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gladyshev, M.I.; Lepskaya, E.V.; Sushchik, N.N.; Makhutova, O.N.; Kalacheva, G.S.

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11.

Low doses of gamma-radiation induce SOS response and increase mutation frequency in Escherichia coli and Salmonella typhimurium cells / A. Bolsunovsky [et al.] // Ecotoxicol. Environ. Saf. - 2016. - Vol. 134. - P233-238, DOI 10.1016/j.ecoenv.2016.09.009 . - ISSN 0147-6513
Кл.слова (ненормированные):
Absorbed dose -- Ames test -- Dose rate -- Mutation rate -- SOS chromotest -- Ames test -- cell death -- DNA repair -- Escherichia coli -- experimental model -- gamma radiation -- limit of detection -- long term exposure -- mutation rate -- nonhuman -- SOS chromotest -- Bacteria (microorganisms) -- Escherichia coli -- Salmonella typhimurium
Аннотация: This study addresses use of two bacterial test systems (the Ames test and the SOS chromotest) to estimate the effects of low doses of γ-radiation. The most substantial increases in induction of SOS response and mutation frequencies were observed in the first 24 h of exposure to γ-radiation as compared to the cells in the exposure-free control. Gamma-radiation also impaired growth and survival of S. typhimurium cells in the first 24 h. The effects were attenuated at lower exposure doses and at longer exposure times. In the experiments conducted in this study, at 96 h of exposure, the values of some of the γ-radiation effects were lower than the MID (minimum inducing dose) detection limits and, thus, were neglected. Long-term exposure to γ-radiation could also result in combined effects of γ-radiation and the death of cells in the culture. © 2016 Elsevier Inc.

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Держатели документа:
Institute of Biophysics of the Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation
FRC Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation
Novosibirsk State University, Novosibirsk, Russian Federation

Доп.точки доступа:
Bolsunovsky, A.; Frolova, T.; Dementyev, D.; Sinitsyna, O.

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12.

Experimental wound dressings of degradable PHA for skin defect repair / E. I. Shishatskaya [et al.] // J. Mater. Sci. Mater. Med. - 2016. - Vol. 27, Is. 11, DOI 10.1007/s10856-016-5776-4 . - ISSN 0957-4530
Кл.слова (ненормированные):
Biomaterials -- Cell culture -- Cells -- Cytology -- Enzyme inhibition -- Fibroblasts -- Membranes -- Natural polymers -- Nonwoven fabrics -- Polymer films -- Proteins -- Stem cells -- Weaving -- Electrospun membranes -- Extracellular matrix protein -- Fibroblast cells -- Hydroxyderivative -- Mesenchymal stem cell -- Non-woven membranes -- Wound dressing materials -- Wound healing process -- Tissue
Аннотация: The present study reports construction of wound dressing materials from degradable natural polymers such as hydroxy derivatives of carboxylic acids (PHAs) and 3-hydroxybutyrate/4-hydroxybutyrate [P(3HB/4HB)] as copolymer. The developed polymer films and electrospun membranes were evaluated for its wound healing properties with Grafts—elastic nonwoven membranes carrying fibroblast cells derived from adipose tissue multipotent mesenchymal stem cells. The efficacy of nonwoven membranes of P(3HB/4HB) carrying the culture of allogenic fibroblasts was assessed against model skin defects in Wistar rats. The morphological, histological and molecular studies revealed the presence of fibroblasts on dressing materials which facilitated wound healing, vascularization and regeneration. Further it was also observed that cells secreted extracellular matrix proteins which formed a layer on the surface of membranes and promoted the migration of epidermal cells from the neighboring tissues surrounding the wound. The wounds under the P(3HB/4HB) membrane carrying cells healed 1.4 times faster than the wounds under the cell-free membrane and 3.5 times faster than the wounds healing under the eschar (control).The complete wound healing process was achieved at Day 14. Thus the study highlights the importance of nonwoven membranes developed from degradable P(3HB/4HB) polymers in reducing inflammation, enhancing angiogenic properties of skin and facilitating better wound healing process. © 2016, Springer Science+Business Media New York.

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Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, 50-50 Akademgorodok, Krasnoyarsk, Russian Federation
Siberian Federal University, 79 Svobodniy Ave., Krasnoyarsk, Russian Federation

Доп.точки доступа:
Shishatskaya, E. I.; Nikolaeva, E. D.; Vinogradova, O. N.; Volova, T. G.

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13.

Genotoxicity assessment of low-level doses of gamma radiation with the SOS chromotest and the Ames test / A. Y. Bolsunovsky [et al.] // Doklad. Biochem. Biophys. - 2016. - Vol. 469, Is. 1. - P309-312, DOI 10.1134/S1607672916040190 . - ISSN 1607-6729
Аннотация: This is the first study to present data on the genotoxicity of low γ-irradiation doses for E. coli and S. typhimurium cells obtained using the SOS chromotest and the Ames test. The most pronounced effect was recorded in the first 24 h of γ-irradiation. After 72 h in the Ames test and after 96 h in the SOS chromotest, a significant effect of γ-irradiation on bacterial cells was detected. The absence of genotoxicity at the later stages can be explained by the adaptation of bacterial cells to the conditions of exposure. The findings allow the bacterial test system to be used for studying the effects of low doses at the early stages of exposure to radiation. © 2016, Pleiades Publishing, Ltd.

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Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, pr. Akademika Lavrent’eva 10, Novosibirsk, Russian Federation
Novosibirsk State University, ul. Pirogova 2, Novosibirsk, Russian Federation
Vorozhtsov Institute of Organic Chemistry, Siberian Branch, Russian Academy of Sciences, pr. Akademika Lavrent’eva 9, Novosibirsk, Russian Federation

Доп.точки доступа:
Bolsunovsky, A. Y.; Sinitsyna, O. I.; Frolova, T. S.; Vasyunina, E. A.; Dementyev, D. V.

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14.

A study of synthesis and properties of poly-3-hydroxybutyrate/diethylene glycol copolymers / T. Volova [et al.] // Biotechnol. Prog. - 2016. - Vol. 32, Is. 4. - P1017-1028, DOI 10.1002/btpr.2267 . - ISSN 8756-7938
Кл.слова (ненормированные):
biocompatibility -- biosynthesis -- molecular weight -- physicochemical and mechanical properties -- poly(3-hydroxybutyrate)/diethylene glycol copolymers -- Biochemistry -- Biocompatibility -- Biosynthesis -- Cell culture -- Cell membranes -- Cells -- Cytology -- Fatty acids -- Glycols -- Molecular weight -- American Institute of Chemical Engineers -- Biological properties -- Chemical compositions -- Cytoplasmic membrane -- Degree of saturations -- Physico-chemical and mechanical properties -- Physiological effects -- Poly-3-hydroxybutyrate -- Biomechanics
Аннотация: This study investigates synthesis of poly(3-hydroxybutyrate)/diethylene glycol copolymers (P3HB/DEG) by Cupriavidus eutrophus B-10646 cells as related to DEG concentration in the medium and the time when it is added to the culture of cells synthesizing P3HB. The study determines the limits of physiological effect of DEG on C. eutrophus cells, showing that at DEG concentrations above 30 g/L, it inhibits cell growth, decreasing cell concentration and total P3HB/DEG yield and inducing an increase in the degree of saturation of fatty acids in lipids of cell cytoplasmic membrane. A series of copolymers containing different molar fractions of DEG (between 0.13 and 3.0 mol%) have been synthesized and their physicochemical, physical/mechanical, and biological properties have been investigated as related to the chemical composition and proportions of DEG monomers of the polymers. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1017–1028, 2016. © 2016 American Institute of Chemical Engineers

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Держатели документа:
Inst. of Biophysics SB RAS, Akademgorodok 50, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Volova, T.; Zhila, N.; Kiselev, E.; Shishatskaya, E.

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15.

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
COMET ASSAY
REPAIR
CELLS
Аннотация: The effect of gamma-radiation on the level of nuclear DNA damage in onion seedlings (Allium-test) was studied using the comet assay. DNA breaks were first found in cells of onion seedlings exposed to low-dose radiation (

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Держатели документа:
Russian Acad Sci, Siberian Branch, Krasnoyarsk Sci Ctr, Inst Biophys, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Inst Cytol & Genet, Siberian Branch, Novosibirsk 630090, Russia.
Novosibirsk State Univ, Novosibirsk 630090, Russia.
Russian Acad Sci, Res Inst Med Genet, Tomsk Natl Res Med Ctr, Tomsk 634058, Russia.

Доп.точки доступа:
Bolsunovsky, A. Ya.; Dementyev, D. V.; Frolova, T. S.; Trofimova, E. A.; Iniatkina, E. M.; Vasilyev, S. A.; Sinitsyna, O. I.; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [18-44-240001]; Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences

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16.

Effects of Gamma-Radiation on DNA Damage in Onion (Allium cepa L.) Seedlings / A. Y. Bolsunovsky, D. V. Dementyev, T. S. Frolova [et al.] // Dokl. Biochem. Biophys. - 2019. - Vol. 489, Is. 1. - P362-366, DOI 10.1134/S1607672919060024 . - ISSN 1608-3091
Аннотация: The effect of ?-radiation on the level of nuclear DNA damage in onion seedlings (Allium-test) was studied using the comet assay. DNA breaks were first found in cells of onion seedlings exposed to low-dose radiation (? 0.1 Gy). Dose dependence of DNA damage parameters showed nonlinear behavior: a linear section in the low-dose region (below 0.1 Gy) and a dose-independent plateau in the dose range between 1 and 5 Gy. Thus, the comet assay can be used to estimate the biological effects of low-dose ?-radiation on Allium cepa seedlings.

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Держатели документа:
Institute of Biophysics, Krasnoyarsk Science Center, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, 660036, Russian Federation
Institute of Cytology and Genetics, Russian Academy of Sciences, Siberian Branch, Novosibirsk, 630090, Russian Federation
Novosibirsk State University, Novosibirsk, 630090, Russian Federation
Research Institute of Medical Genetics, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russian Federation

Доп.точки доступа:
Bolsunovsky, A. Y.; Dementyev, D. V.; Frolova, T. S.; Trofimova, E. A.; Iniatkina, E. M.; Vasilyev, S. A.; Sinitsyna, O. I.

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17.

Reactive Oxygen Species and low-dose effects of tritium on bacterial cells / T. V. Rozhko [et al.] // J. Environ. Radioact. - 2019. - Vol. 208-209. - Ст. 106035, DOI 10.1016/j.jenvrad.2019.106035 . - ISSN 0265-931X
Кл.слова (ненормированные):
Bystander effect -- Low-dose effect -- Luminous marine bacterium -- Radiation hormesis -- Reactive oxygen species -- Signaling molecules -- Tritium -- Bioluminescence -- Cell signaling -- Cells -- Cytology -- Irradiation -- Oxygen -- Phosphorescence -- Physiological models -- Tritium -- Bystander effects -- Low dose effects -- Marine bacterium -- Radiation hormesis -- Reactive oxygen species -- Signaling molecules -- Bacteria -- Bacteria (microorganisms)
Аннотация: The paper continues study of exposures of luminous marine bacteria to low-dose radiation of tritium; tritiated water (HTO) was applied as a source of the irradiation. Hypothesis on involvement of Reactive Oxygen Species (ROS) to signaling mechanism of bacterial cells under exposure to low-intensity tritium radiation was verified. Bacterial bioluminescence intensity was considered as a tested physiological parameter; it was compared to the ROS production in the bacterial environment of different activity concentrations: 0.03, 4.0, and 500 MBq/L. Exposure of the bacteria to chronic low-dose tritium irradiation (<0.08 Gy) increased bioluminescence intensity and ROS production considerably (up to 300%). Spearman rank correlation coefficients were calculated and confirmed relations between the bioluminescence intensity and ROS production. Additional peculiarities of HTO effect were: independence of the bioluminescence intensity and ROS content on HTO activity concentration; low ROS content in bacteria-free aquatic environment. Effects of HTO on bacterial bioluminescence were attributed to: (1) trigger function of tritium decay products in the bacterial metabolic oxygen-dependent processes, with bioluminescence involved; (2) signaling role of ROS as intercellular messengers in “bystander effect”; (3) fixed amount of bacterial cells (3•107 cells/mL) provided the upper limits of the bioluminescence intensity and ROS content. As an outlook, in spite of low energy of tritium decay, its influence on aquatic biota via ROS production by microorganisms should be taken into consideration. © 2019 Elsevier Ltd

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Держатели документа:
Krasnoyarsk State Medical University, P.Zheleznyaka 1, Krasnoyarsk, 660022, Russian Federation
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation
Moscow State University, Department of Chemistry, Moscow119991, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Rozhko, T. V.; Nogovitsyna, E. I.; Badun, G. A.; Lukyanchuk, A. N.; Kudryasheva, N. S.

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