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Microbial degradation of polyhydroxyalkanoates in tropical soils / A. N. Boyandin [et al.] // International Biodeterioration and Biodegradation. - 2013. - Vol. 83. - P77-84, DOI 10.1016/j.ibiod.2013.04.014 . - ISSN 0964-8305
Кл.слова (ненормированные):
Biopolymer properties -- Biopolymers -- PHA degrading microorganisms -- Polyhydroxyalkanoates (PHAs) -- Soil biodegradation -- 3-hydroxybutyric acid -- Degree of crystallinity -- Hydroxyalkanoic acids -- Microbial characteristics -- Microbial degradation -- Polyhydroxyalkanoates -- Polymer molecular mass -- Soil biodegradations -- Bacteriology -- Biomolecules -- Biopolymers -- Microorganisms -- Soils -- Tropics -- Biodegradation -- biodegradation -- chemical composition -- microbial activity -- microbial community -- molecular analysis -- organic compound -- polymer -- soil degradation -- soil microorganism -- tropical soil
Аннотация: The integrated study addressing biodegradation of microbial linear polyesters of hydroxyalkanoic acids (polyhydroxyalkanoates, PHAs) in tropical conditions by microbial communities of Vietnamese soils was performed in locations close to Hanoi and Nha Trang, which differed in their weather conditions and microbial communities. It shows that PHA degradation in tropical soils is influenced by polymer chemical composition, specimen shape, and microbial characteristics. The homopolymer of 3-hydroxybutyric acid is degraded at higher rates than the copolymer of 3-hydroxybutyric and 3-hydroxyvaleric acids. The average rates of mass loss were 0.04-0.33% per day for films and 0.02-0.18% for compact pellets. PHA degradation was accompanied by a decrease in the polymer molecular mass and, usually, an increase in the degree of crystallinity, suggesting preferential degradation of the amorphous phase. Under the study conditions, representatives of the bacterial genera Burkholderia, Bacillus, Cupriavidus, Mycobacterium, and Nocardiopsis and such micromycetes as Acremonium, Gongronella, Paecilomyces, and Penicillium, Trichoderma have been identified as major PHA degraders. В© 2013 Elsevier Ltd.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, Russian Federation
The Joint Russian-Vietnam Tropical Research and Test Center, Hanoi, Viet Nam
Inst. of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Prudnikova, S.V.; Karpov, V.A.; Ivonin, V.N.; D?, N.L.; Nguy?n, T.H.; Le, T.M.H.; Filichev, N.L.; Levin, A.L.; Filipenko, M.L.; Volova, T.G.; Gitelson, I.I.

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The physicochemical properties of polyhydroxyalkanoates with different chemical structures / T. G. Volova [et al.] // Polymer Science - Series A. - 2013. - Vol. 55, Is. 7. - P427-437, DOI 10.1134/S0965545X13070080 . - ISSN 0965-545X
Кл.слова (ненормированные):
Amorphous and crystalline phasis -- Degrees of crystallinity -- Physicochemical property -- Poly(3-hydroxybutirate) -- Polyhydroxyalkanoate copolymer -- Temperature characteristic -- Thermal degradation temperatures -- Thermoplastic materials -- Copolymers -- Monomers -- Thermoplastic elastomers -- Structure (composition)
Аннотация: A set of polyhydroxyalkanoates are synthesized, and a comparative study of their physicochemical properties is performed. The molecular masses and polydispersities of polyhydroxyalkanoates are found to be independent of their chemical structures. It is shown that the temperature characteristics and degrees of crystallinity of polyhydroxyalkanoates are affected by the chemical compositions of the monomers and their quantitative contents in the polymers. The incorporation of 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate units into the chain of poly(3-hydroxybutyrate) decreases its melting point and thermal degradation temperature relative to these parameters of a homogeneous poly(3-hydroxybutyrate) sample (175 В± 5 C and 275 В± 5 C, respectively). The higher the content of the second monomer units in the poly(3-hydroxybutirate) chain, the greater the changes. The degrees of crystallinity of polyhydroxyalkanoate copolymers are generally lower than that of poly(3-hydroxybutyrate) (75 В± 5%). The effect on the ratio of the amorphous and crystalline phases of the copolymer samples becomes more pronounced in the series 3-hydroxy-valerate-3-hydroxyhexanoate-4- hydroxybutyrate. The prepared samples exhibit different properties ranging from rigid thermoplastic materials to engineering elastomers. В© 2013 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50, str. 50, Krasnoyarsk 660036, Russian Federation
Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Svobodnyi pr. 79, Krasnoyarsk 660041, Russian Federation
Siberian State Technological University, pr. Mira 82, Krasnoyarsk 660049, Russian Federation
Kirensky Institute of Physics, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50, str. 38, Krasnoyarsk 660036, Russian Federation
Massachusetts Institute of Technology, Cambridge, MA, United States : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Zhila, N.O.; Shishatskaya, E.I.; Mironov, P.V.; Vasil'Ev, A.D.; Sukovatyi, A.G.; Sinskey, A.J.

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Effects of intracellular poly(3-hydroxybutyrate) reserves on physiological-biochemical properties and growth of Ralstonia eutropha / T. G. Volova [et al.] // Research in Microbiology. - 2013. - Vol. 164, Is. 2. - P164-171, DOI 10.1016/j.resmic.2012.10.008 . - ISSN 0923-2508
Кл.слова (ненормированные):
Granules -- PHA cycle -- Poly(3-hydroxybutyrate) -- Ralstonia eutropha -- poly(3 hydroxybutyric acid) -- article -- bacterial growth -- bacterial strain -- bacterium culture -- cell division -- cell granule -- cell size -- controlled study -- Cupriavidus necator -- electron microscopy -- nonhuman -- polymer production -- priority journal -- Cupriavidus necator -- Cytoplasmic Granules -- Hydroxybutyrates -- Microscopy, Electron, Transmission -- Polyesters -- Bacteria (microorganisms) -- Cupriavidus necator
Аннотация: Microbial polyhydroxyalkanoates (PHAs), because of their well studied complex physiology and commercial potential, are vehicles for carbon and potential storage reduction for many microbial species. Even with the wealth of studies about microbial PHAs in the scientific literature, polymer accumulation and degradation are still not comprehensively understood. Poly(3-hydroxybutyrate) (P3HB) granule formation and polymer mobility were studied here in the bacterium Ralstonia eutropha strain B5786 in autotrophic cultures. Electron microscopy studies revealed decreasing cell size concomitant with enlargement of size and number of intracellular granules, and inhibition of cell division during intracellular polymer production. Activities of key P3HB biosynthetic enzymes demonstrated correlations with each other during polymer accumulation, suggesting an intricately regulated P3HB cycle in autotrophically grown R. eutropha cells. В© 2012 .

Scopus
Держатели документа:
Laboratory of Chemoautotrophic Biosynthesis, Institute of Biophysics of the Siberian Branch of The Russian Academy of Sciences, Akademgorodok 50, Krasnoyarsk 660036, Russian Federation
Department of Biotechnology, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Avenue, Krasnoyarsk 660041, Russian Federation
Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge 02139, MA, United States
Health Sciences Technology Division, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge 02139, MA, United States
Engineering Systems Division, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge 02139, MA, United States : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Zhila, N.O.; Kalacheva, G.S.; Brigham, C.J.; Sinskey, A.J.

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An in vivo study of pha matrices of different chemical composition: Tissue reaction and biodegradation / E. I. Shishatskaya [et al.] // Cellular Transplantation and Tissue Engineering. - 2012. - Vol. 7, Is. 1. - P73-80 . - ISSN 1815-445X
Кл.слова (ненормированные):
Degradable polymer matrices -- Degradation -- PHAs -- Polyhydroxyalkanoates -- Subcutaneous implantation -- Tissue reaction
Аннотация: The study addresses consequences of subcutaneous implantation of film matrices prepared from different PHAs to laboratory animals. No negative effects of subcutaneous implantation of PHA matrices on physiological and biochemical characteristics of the animals were determined. Independently of the matrices composition and duration of the contact with the internal environment of the organism we did not observe any deviations in the behavior of animals, their growth and development, as well as blood functions. Response of the tissues to PHA matrices was comparable with the response to polylactide, but substantially less expressed at the earlier time periods after implantation. Tissues response to implantation of PHA of all types is characterized by short-term (up to 2 weeks) post-traumatic inflammation with formation of fibrous capsules by 30th-60th days with the thickness less than 100 microns, which get thinner down to 40-60 microns by 180th day as the result of involution. No differences in response of tissues and the whole organism were observed for the matrices produced from the homopolymer of 3-hydroxybutyric acid (P3HB), copolymers of 3-hydroxybutyric and 4-hydroxybutyric acids (P3HB/4HB), 3-hydroxybutyric acid and 3-hydroxyvalerianic acids (P3HB/3HV), 3-hydroxybutyric and 3-hydroxyhexanoate acids (P3HB/3HH). Macrophages and foreign-body giant cells actively participate in the response of the tissues to PHAs. In the studied conditions matrices from the copolymers containing 3-hydroxyhexanoate and 4 hydroxybutyrate were determined as more actively degraded PHA. The next less degraded matrices were matrices from the copolymer of P3HB/3HV and the most resistant were P3HB matrices. The slower degradation of PHA matrices was accompanied by delayed development of giantcells response. The studied PHA matrices can be placed in the following range by their degradation: P3HB/3HH - P3HB/4HB - P3HB/HV - P3HB.

Scopus
Держатели документа:
Institute of Modern Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, Krasnoyarsk, Russian Federation
Massachusetts Institute of Technology, Cambridge, United States : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Nikolaeva, E.D.; Goreva, A.V.; Brigham, C.J.; Volova, T.G.; Sinskey, A.J.

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Biodegradation of polyhydroxyalkanoate films in natural environments / A. N. Boyandin [et al.] // Macromolecular Symposia. - 2012. - Vol. 320, Is. 1. - P38-42, DOI 10.1002/masy.201251004 . - ISSN 1022-1360
Кл.слова (ненормированные):
biodegradation -- biopolymers -- microbial degradation -- polyhydroxyalkanoates -- Acinetobacters -- Acremonium -- Alcaligenes -- Burkholderia -- Degradation rate -- Degrading activities -- Degrading bacteria -- Enterobacter -- Hydroxyvalerate -- Mass loss -- Microbial degradation -- Micromycetes -- Natural environments -- Paecilomyces -- Poly-3-hydroxybutyrate -- Poly-hydroxyalkanoate -- Polyhydroxyalkanoates -- SIBERIA -- South China sea -- Stenotrophomonas -- Trichoderma -- Tropical soils -- Viet Nam -- Xanthomonas -- Bacteria -- Bacteriology -- Biopolymers -- Degradation -- Seawater -- Soils -- Tropics -- Biodegradation
Аннотация: Biodegradation of film specimens from polyhydroxyalkanoates (PHAs) of two types - poly-3-hydroxybutyrate (PHB) and poly-3-hydroxybutyrate-co-3- hydroxyvalerate (PHBV) - was analysed in different environments: tropical sea waters of the South China Sea (Nha Trang, Vietnam) and soils in the environs of Hanoi (Vietnam), Nha Trang (Vietnam) and Krasnoyarsk (Siberia, Russia). In seawater, the mass loss of the specimens of both types was almost equal. However, in tropical soils, PHB degraded quicker than PHBV. In the Siberian soil, the degradation rate of the PHBV was generally higher than that of PHBV. Analysis of molecular mass of PHA specimens showed its decreasing during biodegradation. In the tropical sea conditions, PHA degrading microorganisms were represented by bacteria of Enterobacter, Bacillus and Gracilibacillus genera. Among PHA degrading bacteria, Burkholderia, Alcaligenes, Bacillus, Mycobacterium and Streptomyces genera were identified in Vietnamese soils, and Variovorax, Stenotrophomonas, Acinetobacter, Pseudomonas, Bacillus and Xanthomonas genera in Siberian soils. Micromycetes of Gongronella, Paecilomyces, Penicillium and Trichoderma genera exhibited PHA degrading activity in Vietnamese soils, and Paecilomyces, Penicillium, Acremonium, Verticillium and Zygosporium genera - in Siberian soils. Copyright В© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Scopus
Держатели документа:
Institute of Biophysics, SB RAS, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk, 660041, Russian Federation
Joint Vietnam-Russian Tropical Research and Technological Centre, Nguyen Van Huyen, Nghia Do, Cau Giay, Hanoi, Viet Nam
Institute of Chemical Biology and Fundamental Medicine, SB RAS, 8 Lavrentiev Ave., Novosibirsk, 630090, Russian Federation
Massachusetts Institute of Technology, Cambridge, MA 02139, United States : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Rudnev, V.P.; Ivonin, V.N.; Prudnikova, S.V.; Korobikhina, K.I.; Filipenko, M.L.; Volova, T.G.; Sinskey, A.J.

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Biodegradation of polyhydroxyalkanoates (PHAs) in the South China Sea and identification of PHA-degrading bacteria / T. G. Volova [et al.] // Microbiology. - 2011. - Vol. 80, Is. 2. - P252-260, DOI 10.1134/S0026261711020184 . - ISSN 0026-2617
Кл.слова (ненормированные):
biodegradation in marine environments -- microbial PHA degraders -- PHA -- polyhydroxyalkanoates -- Bacillus (bacterium) -- Bacillus sp. -- Bacteria (microorganisms) -- Enterobacter -- Enterobacter cloacae -- Gracilibacillus -- Prospect Hill virus
Аннотация: The biodegradation patterns of two types of PHA, a 3-hydroxybutyrate (3-PHB) polymer and a 3-hydroxybutyrate and 3-hydroxyvalerate (3-PHB/3-PHV) copolymer, were studied in tropical marine environments (Dam Bay, South China Sea, Nha Trang, Vietnam). No reliable differences in the degradation of 3-PHB and 3-PHB/3-PHV were revealed. It was shown that the degradation process depended mainly on the shape of a polymer product and its production method: the degradation of polymer films was found to be more active than that of molded solids. A decrease in the molecular mass of both types of PHA was detected in the course of the degradation of PHA samples. However, the degree of PHA crystallinity did not change; that is, the levels of degradation of both the amorphous and crystalline phases of PHA were almost the same. Among microbial PHA degraders, three bacterial strains, Bacillus sp. IBP-V002, Enterobacter cloacae sp. IBP-V001, and Gracilibacillus sp. IBP-V003, were identified based on the results of morphological, biochemical, and molecular phylogenetic analyses. The ability of the representatives of the genera Gracilibacillus and Enterobacter to degrade PHA was revealed for the first time. В© 2011 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Kirensky Institute of Physics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, Russian Federation
Marine Department, Joint Russian-Vietnamese Tropical Research and Test Center, Nha Trang, Viet Nam : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Boyandin, A.N.; Vasil'ev, A.D.; Karpov, V.A.; Kozhevnikov, I.V.; Prudnikova, S.V.; Rudnev, V.P.; Xuan, B.B.; Dung, V.V.; Gitel'zon, I.I.

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Biodegradation of polyhydroxyalkanoates (PHAs) in tropical coastal waters and identification of PHA-degrading bacteria / T. G. Volova [et al.] // Polymer Degradation and Stability. - 2010. - Vol. 95, Is. 12. - P2350-2359, DOI 10.1016/j.polymdegradstab.2010.08.023 . - ISSN 0141-3910
Кл.слова (ненормированные):
Biopolymers in marine environment -- Degradation of polymers -- PHA properties -- PHA-degrading microorganisms -- Polyhydroxyalkanoates, PHA -- 16S rRNA -- 3-hydroxybutyric acid -- Amorphous phase -- Bacillus sp -- Biodegradation rate -- Chemical compositions -- Coastal waters -- Degradation rate -- Degrading bacteria -- Degree of crystallinity -- Enterobacter -- Hydroxyvalerate -- Marine environment -- PHA properties -- PHA-degrading microorganisms -- Polydispersity indices -- Polyhydroxyalkanoates -- Polymer chains -- Preparation technique -- Sole carbon source -- South China Sea -- Viet Nam -- Acids -- Bacteriology -- Biodegradable polymers -- Biodegradation -- Biomolecules -- Biopolymers -- Microorganisms -- Organic polymers -- Polydispersity -- Polymer films -- Polymers -- RNA -- Seawater -- Degradation
Аннотация: Biodegradability patterns of two PHAs: a polymer of 3-hydroxybutyric acid (3-PHB) and a copolymer of 3-hydroxybutyric and 3-hydroxyvaleric acids (3-PHB/3-PHV) containing 11 mol% of hydroxyvalerate, were studied in the tropical marine environment, in the South China Sea (Nha Trang, Vietnam). No significant differences have been observed between degradation rates of 3-PHB and 3-PHB/3-PHV specimens; it has been found that under study conditions, biodegradation is rather influenced by the shape of the polymer item and the preparation technique than by the chemical composition of the polymer. Biodegradation rates of polymer films in seawater have been found to be higher than those of compacted pellets. As 3-PHB and 3-PHB/3-PHV are degraded and the specimens lose their mass, molecular weight of both polymers is decreased, i.e. polymer chains get destroyed. The polydispersity index of the PHAs grows significantly. However, the degree of crystallinity of both PHAs remains unchanged, i.e. the amorphous phase and the crystalline one are equally disintegrated. PHA-degrading microorganisms were isolated using the clear-zone technique, by inoculating the isolates onto mineral agar that contained PHA as sole carbon source. Based on the 16S rRNA analysis, the PHA-degrading strains were identified as Enterobacter sp. (four strains), Bacillus sp. and Gracilibacillus sp.В© 2010 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, 79 Svobodny av., Krasnoyarsk 660041, Russian Federation
L.V. Kirensky Institute of Physics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation
A.N. Severtsov Institute of Ecology and Evolution RAS, 33 Leninskij Prospect, Moscow 119071, Russian Federation
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, 8 Lavrentiev Ave., Novosibirsk 630090, Russian Federation
Joint Russian-Vietnam Tropical Research and Test Centre, Coastal Branch, 30 Nguyen Thien Thuat, Nha Trang, Viet Nam : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Boyandin, A.N.; Vasiliev, A.D.; Karpov, V.A.; Prudnikova, S.V.; Mishukova, O.V.; Boyarskikh, U.A.; Filipenko, M.L.; Rudnev, V.P.; Ba Xuan, B.; Vit Dung, V.; Gitelson, I.I.

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Distribution and resorption of polymeric microparticles in visceral organs of laboratory animals after intravenous injection / E. I. Shishatskaya [et al.] // Bulletin of Experimental Biology and Medicine. - 2009. - Vol. 148, Is. 5. - P789-793, DOI 10.1007/s10517-010-0817-3 . - ISSN 0007-4888
Кл.слова (ненормированные):
14C -- Intravenous injection -- Microparticles -- Resorbable polyhydroxybutyrate -- Visceral tissues -- carbon 14 -- microsphere -- polyhydroxyaminobutyric acid -- polymer -- unclassified drug -- animal experiment -- animal tissue -- article -- biocompatibility -- biodegradability -- body growth -- controlled drug release -- controlled study -- degradation kinetics -- drug delivery system -- drug dosage form -- experimental animal -- female -- liver parenchyma -- molecular weight -- nonhuman -- particle size -- physical development -- radioactivity -- rat -- spleen -- tissue distribution -- tissue structure -- Animals -- Animals, Laboratory -- Carbon Radioisotopes -- Female -- Injections, Intravenous -- Particle Size -- Polymers -- Rats -- Rats, Wistar -- Tissue Distribution -- Viscera -- Animalia
Аннотация: Microparticles obtained by using 14C-labeled resorbable hydroxyaminobutyric acid polymer were injected into the caudal vein of laboratory animals without negative aftereffects for their growth and development and without changes in the macro- and microstructure of organs and tissues. The distribution of microparticles in the viscera and the dynamics of accumulation of carbon-containing polymer degradation products in the viscera were studied. The main targets for the particles are liver tissues, as well as renal and splenic tissues. The polymeric matrix of the microparticles is most actively destroyed in the spleen and liver. The presence of high-molecular- weight polymeric matrix in organs indicates the integrity of microparticles and the possibility of long-term (up to 12 weeks) functioning of polymeric particles in vivo. В© 2009 Springer Science+Business Media, Inc.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Institute of Basic Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Goreva, A.V.; Voinova, O.N.; Kalacheva, G.S.; Volova, T.G.

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Microbial polymers as a degradable carrier for pesticide delivery / O. N. Voinova [et al.] // Applied Biochemistry and Microbiology. - 2009. - Vol. 45, Is. 4. - P384-388, DOI 10.1134/S0003683809040061 . - ISSN 0003-6838
Аннотация: The possibility of use of polyhydroxyalkanoates (PHAs), biodegradable microbial polyesters, as a carrier for pesticides (?-hexachlorcyclohexane and lindane) for targeted and controlled delivery of these compounds to soil was investigated. The kinetics of polymer degradation and the dynamics of pesticide release from the extended-release formulations was studied. It is shown that pesticides embedded in a degradable polymer (PHA) carrier are released gradually and slowly, without surges, as the polymer is degraded by the soil micro-flora. The microbial soil component actively responded to the addition of the polymer as an additional nutrient substrate: the latter was degraded and then utilized. The rate of the pesticide release to the soil can be regulated by varying the polymer-pesticide ratio. В© 2009 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation
Institute of Forestry, Siberian Branch Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Voinova, O.N.; Kalacheva, G.S.; Grodnitskaya, I.D.; Volova, T.G.

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10.

Comparative study of PHA degradation in natural reservoirs having various types of ecosystems / O. Voinova, M. Gladyshev, T. G. Volova // Macromolecular Symposia. - 2008. - Vol. 269, Is. 1. - P34-37, DOI 10.1002/masy.200850906 . - ISSN 1022-1360
Кл.слова (ненормированные):
Biodegradable polyesters -- Ecosystem -- Natural environment -- PHA -- Water reservoir -- ABS resins -- Biochemistry -- Biodegradable polymers -- Biodegradation -- Biopolymers -- Biotechnology -- Degradation -- Ecology -- Ecosystems -- Esters -- Polymers -- Aerobic and anaerobic conditions -- Anaerobic conditions -- Biodegradable polyesters -- Comparative studies -- Ecological characteristics -- Environment temperatures -- Natural environment -- Natural environments -- PHA -- Polyhydroxyalkanoate -- Polymer degradations -- Water reservoir -- Water reservoirs -- Reservoirs (water)
Аннотация: The kinetics of polyhydroxyalkanoate (PHA) degradation in natural environment in two water reservoirs having various ecological characteristics was studied. It was shown that biodegradation of polymer essentially depends on the environment temperature and inorganic composition of water. The processes of polymer degradation under aerobic and anaerobic conditions were compared. The polymer degradation was slower under anaerobic conditions. Copyright В© 2008 WILEY-VCH Verlag GmbH & Co. KGaA.

Scopus
Держатели документа:
Institute of Biophysics, SB, RAS, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Siberian Federal National University, Svohodnyi Av., Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Voinova, O.; Gladyshev, M.; Volova, T.G.

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11.

Degradation of polyhydroxyalkanoates in eutrophic reservoir / T. G. Volova [et al.] // Polymer Degradation and Stability. - 2007. - Vol. 92, Is. 4. - P580-586, DOI 10.1016/j.polymdegradstab.2007.01.011 . - ISSN 0141-3910
Кл.слова (ненормированные):
16S rDNA -- Degradation -- DGGE -- Polyhydroxyalkanoates -- Reservoir -- Biodegradation -- Climate change -- Ecosystems -- Eutrophication -- Genetic engineering -- Physical chemistry -- Eutrophic reservoir -- Phylogenetic analysis -- Physicochemical properties -- Polyhydroxyalkanoates -- Organic polymers
Аннотация: During the summers of 1999-2001 the dynamics of polyhydroxyalkanoate degradation in a small recreational eutrophic reservoir was studied experimentally. It has been shown that biodegradation of polyhydroxyalkanoates in the environment is determined by the structure and physicochemical properties of the polymer and by local weather conditions, which influence the state of the aquatic ecosystem. Species (clones) of bacteria able to utilize polyhydroxyalkanoates in the reservoir were identified using molecular phylogenetic analysis of 16S rRNA genes. В© 2007 Elsevier Ltd. All rights reserved.

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Держатели документа:
Siberian Federal University, Svobodny av. 79, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Gladyshev, M.I.; Trusova, M.Y.; Zhila, N.O.

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12.

Degradation of polyhydroxyalkanoates and the composition of microbial destructors under natural conditions / T. G. Volova [et al.] // Microbiology. - 2006. - Vol. 75, Is. 5. - P593-598, DOI 10.1134/S0026261706050092 . - ISSN 0026-2617
Кл.слова (ненормированные):
16S rRNA -- Biodegradation -- Identification -- Polyhydroxyalkanoates -- Bacteria (microorganisms)
Аннотация: The degradation dynamics of polyhydroxyalkanoates of different composition has been studied in an eutrophic storage reservoir for two seasons. It has been shown that the biodegradation of polymers under natural conditions depends not only on their structure and physicochemical properties but also, to a great extent, on a complex of weather-climatic conditions affecting the state of the reservoir ecosystem. The molecular genetic analysis of 16S rRNA has revealed bacterial species (clones) probably involved in the degradation of polyhydroxyalkanoates in a model storage reservoir. В© Nauka/Interperiodica 2006.

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Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Gladyshev, M.I.; Trusova, M.Yu.; Zhila, N.O.

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13.

Degradation of P(3HB) and P(3HB-co-3HV) in biological media / E. I. Shishatskaya [et al.] // Journal of Biomaterials Science, Polymer Edition. - 2005. - Vol. 16, Is. 5. - P643-657, DOI 10.1163/1568562053783678 . - ISSN 0920-5063
Кл.слова (ненормированные):
Degradation rate -- Fiber properties -- Morphology -- Poly(hydroxybutyrate-co-hydroxyvalerate) (P(3HB-co-3HV)) -- Polyhydroxyalkanoates(PHAs) -- Polyhydroxybutyrate (P(3HB)) -- Copolymers -- Degradation -- Differential scanning calorimetry -- Enzymes -- Morphology -- Scanning electron microscopy -- Tensile strength -- Tissue -- Transmission electron microscopy -- Degradation rate -- Fiber properties -- Polyhydroxyalkanoates (PHAs) -- Polyhydroxybutyrate (P(3HB)) -- Biopolymers -- buffer -- copolymer -- poly(3 hydroxybutyric acid) -- polyhydroxybutyrate hydroxyvalerate copolymer -- unclassified drug -- animal experiment -- animal model -- animal tissue -- article -- biodegradation -- controlled study -- crystal structure -- fiber -- giant cell -- in vitro study -- in vivo study -- macrophage -- morphology -- nonhuman -- pH -- priority journal -- rat -- structure analysis -- tensile strength -- tissue water -- weight reduction -- Animals -- Biodegradation, Environmental -- Buffers -- Humans -- Hydrogen-Ion Concentration -- Hydroxybutyrates -- Macrophages -- Microscopy, Electron, Scanning -- Microscopy, Electron, Transmission -- Muscle, Skeletal -- Polyesters -- Rats -- Rats, Wistar
Аннотация: The biodegradability of oriented fibers made of polyhydroxybutyrate (P(3HB)) and its co-polymer with ?-hydroxyvalerate (P(3HB-co-3HV)) was investigated in buffer solutions and in biological media in vitro and in vivo. The fibers of both polymer types demonstrated resistance to hydrolytic degradation in buffer solutions at 38В°C and pH from 4.5 to 7.0 (for up to 180 days). It has been found that the biodegradation of the fibers in vitro in blood and serum and in vivo is accompanied by weight losses and minor changes in the microstructure with no significant losses in the tensile strength over a long time (up to 180 days). The biodegradation rate of the less crystalline co-polymer P(3HB-co-3HV) fibers was 1.4-2.0-times higher than that of the homopolymer P(3HB). It has also been shown that the degradation of the fibers in vivo is influenced both by tissue fluid enzymes and cells (macrophages and foreign-body giant cells). The fibers were eroded on the surface only with no gross defects and no dramatic effects on their mechanical performance. В© VSP 2005.

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Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 600326, Russian Federation
Department of Cardiac Surgery, University of Glasgow, Royal Infirmary, 10 Alexandra Parade, Glasgow G3, United Kingdom : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Gordeev, S.A.; Puzyr, A.P.

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14.

Degradation of bioplastic in environment / T. G. Volova [и др.] // Doklady Akademii Nauk. - 2004. - Vol. 397, Is. 5. - С. 708-710 . - ISSN 0869-5652
Кл.слова (ненормированные):
Bacteria -- Ecology -- Environmental testing -- Plastics -- Weathering -- Time dependence -- Biomaterials
Аннотация: For the first time degradation kinetics is investigated for polyhydroxyalkanoates (PHA) in the native fresh pond. Its substantial variability in dependence on ecological conditions is shown, new bacteria species (clones) capable for PHA utilization in fresh-water ecosystem are identified.

Scopus
Держатели документа:
Inst. Biofiziki SO RAN, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Golyshev, M.I.; Trusova, M.Yu.; Zhila, N.O.; Kartushinskaya, M.V.

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15.

Degradation of bioplastics in natural environment. / T. G. Volova [et al.] // Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections / translated from Russian. - 2004. - Vol. 397. - P330-332 . - ISSN 0012-4966
Кл.слова (ненормированные):
biopolymer -- plastic -- article -- bioremediation -- chemistry -- environment -- kinetics -- materials -- Russian Federation -- water supply -- Biodegradation, Environmental -- Biopolymers -- Environment -- Kinetics -- Manufactured Materials -- Plastics -- Russia -- Water Supply

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Gladyshev, M.I.; Trusova, M.Y.; Zhila, N.O.; Kartushinskaya, M.V.

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16.

Dynamics of activity of the key enzymes of polyhydroxyalkanoate metabolism in Ralstonia eutropha B5786 / T. G. Volova [et al.] // Applied Biochemistry and Microbiology. - 2004. - Vol. 40, Is. 2. - P170-177, DOI 10.1023/B:ABIM.0000018921.04863.d5 . - ISSN 0003-6838
Кл.слова (ненормированные):
acetyl coenzyme A acyltransferase -- bacterial enzyme -- carbon -- carbon dioxide -- fructose -- hydrogen -- hydroxybutyrate dehydrogenase -- oxidoreductase -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- synthetase -- article -- bacterial metabolism -- carbon source -- catalysis -- controlled study -- degradation -- depolymerization -- enzyme activity -- enzyme analysis -- molecular dynamics -- nonhuman -- protein function -- Ralstonia eutropha -- recording -- statistical significance -- synthesis -- Bacteria (microorganisms) -- Ralstonia -- Wautersia eutropha
Аннотация: The dynamics of accumulation of polyhydroxybutyrate (PHB) and the activities of key enzymes of PHB metabolism (?-ketothiolase, acetoacetyl-CoA reductase, PHB synthase, D-hydroxybutyrate dehydrogenase, and PHB depolymerase) in the hydrogen bacterium Ralstonia eutropha B5786 were studied under various conditions of carbon nutrition and substrate availability. The highest activities of ?-ketothiolase, acetoacetyl-CoA reductase, and PHB synthase were recorded during acceleration of PHB synthesis. The activities of enzymes catalyzing PHB depolymerization (PHB depolymerase and D-hydroxybutyrate dehydrogenase) were low, being expressed only upon stimulated endogenous PHB degradation. The change of carbon source (CO2 or fructose) did not affect the time course of the enzyme activity significantly.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Gorbunova, O.V.; Zhila, N.O.

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17.

Dynamics of activity of the key enzymes of polyhydroxyalkanoate metabolism in Ralstonia eutropha / T. G. Volova [и др.] // Prikladnaia biokhimiia i mikrobiologiia. - 2004. - Vol. 40, Is. 2. - С. 201-209 . - ISSN 0555-1099
Кл.слова (ненормированные):
acetoacetyl coenzyme a reductase -- acetoacetyl-CoA reductase -- acetyl coenzyme A acyltransferase -- acyltransferase -- alcohol dehydrogenase -- carboxylesterase -- hydroxybutyrate dehydrogenase -- hydroxybutyric acid -- poly(3 hydroxyalkanoic acid) depolymerase -- poly(3-hydroxyalkanoic acid) depolymerase -- poly(3-hydroxyalkanoic acid) synthase -- polyhydroxyalkanoate synthase -- polymer -- article -- chemistry -- comparative study -- culture medium -- enzymology -- growth, development and aging -- metabolism -- Wautersia eutropha -- Acetyl-CoA C-Acyltransferase -- Acyltransferases -- Alcohol Oxidoreductases -- Carboxylic Ester Hydrolases -- Culture Media -- Cupriavidus necator -- Hydroxybutyrate Dehydrogenase -- Hydroxybutyrates -- Polymers
Аннотация: The dynamics of accumulation of polyhydroxybutyrate (PHB) and the activities of the key enzymes of PHB metabolism (beta-ketothiolase, acetoacetyl-CoA reductase, PHA synthase, D-hydroxybutyrate dehydrogenase, and PHA depolymerase) in the hydrogen bacterium Ralstonia eutropha B5786 were studied under various conditions of carbon nutrition and substrate availability. The highest activities of beta-ketothiolase, acetoacetyl-CoA reductase, and PHA synthase were recorded at the stage of acceleration of PHB synthesis. The activities of enzymes catalyzing PHB depolymerization (PHB depolymerase and D-hydroxybutyrate dehydrogenase) were low, being expressed only at stimulated endogenous PHB degradation. The change of carbon source (CO2 or fructose) did not cause any marked changes in the time course of enzyme activity.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Gorbunova, O.V.; Zhila, N.O.

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18.

Biocompatibility and resorption of intravenously administered polymer microparticles in tissues of internal organs of laboratory animals / E. Shishatskaya [et al.] // Journal of Biomaterials Science, Polymer Edition. - 2011. - Vol. 22, Is. 16. - P2185-2203, DOI 10.1163/092050610X537138 . - ISSN 0920-5063
Кл.слова (ненормированные):
14C products of polymer resorption -- intravenous administration -- microparticles -- Resorbable polyhydroxybutyrate -- tissues of internal organs -- 3-hydroxybutyric acid -- sup14/supC products of polymer resorption -- Adverse effect -- Drug-delivery systems -- Growth and development -- High molecular weight -- Initial molecular weight -- Initial values -- Internal organs -- Intravenous administration -- Laboratory animals -- Local response -- matrix -- Mean diameter -- Micro-particles -- Polymer degradation -- Polymer microparticles -- Preparation process -- Radioactivity level -- Residual content -- Resorbable -- Sustained-release -- Tail veins -- Wistar rat -- Animals -- Biocompatibility -- Degradation -- Histology -- Molecular weight -- Morphology -- Radiation -- Radioactivity -- Rats -- Tissue -- poly(3 hydroxybutyric acid) -- animal experiment -- animal tissue -- article -- biocompatibility -- concentration process -- controlled study -- degradation -- drug delivery system -- female -- gel permeation chromatography -- heart -- kidney parenchyma -- liver -- lung parenchyma -- molecular weight -- nonhuman -- organ culture -- priority journal -- radioactivity -- rat -- spleen -- Animals -- Animals, Laboratory -- Biocompatible Materials -- Drug Delivery Systems -- Female -- Infusions, Intravenous -- Materials Testing -- Microspheres -- Molecular Weight -- Particle Size -- Polymers -- Rats -- Rats, Wistar -- Tissue Distribution -- Animalia -- Rattus -- Rattus norvegicus
Аннотация: Specimens of 14C-labeled polymer of 3-hydroxybutyric acid, P(3-HB), with different initial molecular weights, were used to prepare microparticles, whose morphology was not influenced by the M w of the polymer. During the particle preparation process, P(3-HB) molecular weight decreased by 15-20%. Sterile microparticles (mean diameter 2.4 ?m) were injected into the tail veins of Wistar rats (5 mg/rat). The effects of the particles administered to rats were studied based on the general response of animals and local response of internal organ tissues and blood morphology; no adverse effects on growth and development of the animals or unfavorable changes in the structure of the tissues of internal organs were observed. Measurements of radioactivity in tissues showed that 14C concentrations are different in different organs, changing during the course of the experiment. The main targets for 14C-labeled microparticles were tissues of the liver, spleen, and kidneys. Comparison of radioactivity levels and residual contents of high-molecular-weight matrix in tissues suggested that the most rapid metabolism and degradation of P(3-HB) occurred in the liver and spleen. Gel-permeation chromatography showed that at 3 h after the microparticles were injected into the bloodstream, polymer degradation started in all examined organs, except the lungs; at 12 weeks, the M w of the polymer matrix was as low as 20-30% of its initial value. The presence of high-molecular-weight (undegraded) polymer in the tissues at 12 weeks after administration of the particles suggests that P(3-HB) is degraded in tissues of internal organs slowly and, hence, P(3-HB)-based microparticles can be used as sustained-release drug-delivery systems. В© 2011 VSP.

Scopus
Держатели документа:
Institute of Biophysics, SB RAS, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, Svobodnyi Avenue, Krasnoyarsk 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.; Goreva, A.; Kalacheva, G.; Volova, T.

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19.

Kinetic characteristics of the theoretical ecosystems with different extent of openness / Ya. V. Galayda [et al.] // Advances in Space Research. - 2005. - Vol. 35, Is. 9 SPEC. ISS. - P1516-1520, DOI 10.1016/j.asr.2005.01.034 . - ISSN 0273-1177
Кл.слова (ненормированные):
Mathematic modeling -- Net primary production -- Residual concentration of biogenic limiting substances -- Trophic chain length -- Mathematic modeling -- Net primary production -- Residual concentration of biogenic limiting substances -- Trophic chain length -- Characterization -- Degradation -- Kinetic theory -- Mathematical models -- Nitrogen -- Phosphorus -- Recycling -- Surface waters -- Ecosystems -- biological model -- biomass -- bioremediation -- conference paper -- ecosystem -- food chain -- microclimate -- Biodegradation, Environmental -- Biomass -- Ecological Systems, Closed -- Ecosystem -- Food Chain -- Life Support Systems -- Models, Biological
Аннотация: In this paper, the influence of the extent of openness of ecosystem that is defined by the dilution rate, which characterizes the extent of flowage of the pond, on the intensity of the biotic circulation in ecosystems with different regulation types, number of trophic links and extent of closing has been investigated. We considered open systems, we took into account the return of the limiting substances, such as nitrogen and phosphorous, into the cycle by degradation of detritus and products of vital functions of consumers. It was shown by the numerical calculations that the increase of the dilution rate in without recycle ecosystems leads to increase of the net primary production up to the maximum value corresponding to the two-link trophic chain (biogenic substance and producer) and then, to gradually decrease. The residual concentration of biogenic limiting substances monotone increases. Net primary production and residual concentration of biogenic limiting substances in systems with recycle with even number of links behaves similarly to that in without recycle ecosystems. In the systems with recycle with the odd number of links that values lies on the stable level. We showed that in wide range of the dilution rate the recycling of the ecosystem can highly increase the net primary production and reduce residual concentration of biogenic limiting substances. The influence of the dilution rate on numbers of links that may exist in the system was analyzed. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Krasnoyarsk State University, Svobodny 79, Krasnoyarsk 660041, Russian Federation
Institute of Biophysics, SB, RAS, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Galayda, Ya.V.; Pechurkin, N.S.; Shirobokova, I.M.; Pisman, T.I.

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20.

Material balance and diet in bioregenerative life support systems: Connection with coefficient of closure / N. S. Manukovsky [et al.] // Advances in Space Research. - 2005. - Vol. 35, Is. 9 SPEC. ISS. - P1563-1569, DOI 10.1016/j.asr.2005.01.002 . - ISSN 0273-1177
Кл.слова (ненормированные):
Bioregenerative life support system -- Coefficient of closure -- Diet -- Soil-like substrate -- Additives -- Degradation -- Materials balance -- Nitrogen -- Nutrition -- Stoichiometry -- Bioregenerative life support systems -- Coefficient of closure -- Soil-like substrates -- Life support systems (spacecraft) -- vegetable protein -- biomass -- conference paper -- culture medium -- diet -- feces -- growth, development and aging -- human -- methodology -- microclimate -- plant -- urine -- waste management -- Biomass -- Culture Media -- Diet -- Ecological Systems, Closed -- Feces -- Humans -- Life Support Systems -- Plant Proteins -- Plants, Edible -- Urine -- Waste Management
Аннотация: Bioregenerative life support systems (BLSS) with different coefficients of closure are considered. The 66.2% coefficient of closure achieved in "BIOS-3" facility experiments has been taken as a base value. The increase in coefficient of closure up to 72.6-93.0% is planned due to use of soil-like substrate (SLS) and concentrating of urine. Food values were estimated both in a base variant ("BIOS-3"), and with increases in the coefficient of closure. It is shown that food requirements will be more fully satisfied by internal crop production with an increase in the coefficient of closure of the BLSS. Changes of massflow rates on an 'input-output' and inside BLSS are considered. Equations of synthesis and degradation of organic substances in BLSS were examined using a stoichiometric model. The paper shows that at incomplete closure of BLSS containing SLS there is a problem of nitrogen balancing. To compensate for the removal of nitrogen from the system in urine and feces, it is necessary to introduce food and a nitrogen-containing additive. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Academgorodok, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Manukovsky, N.S.; Kovalev, V.S.; Somova, L.A.; Gurevich, Yu.L.; Sadovsky, M.G.

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