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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gurevich Y.L., Ladygina V.P.
Заглавие : Effect of protozoa on bacterial degradation of aromatic hydrocarbons
Место публикации : Studies in Environmental Science. - 1991. - Vol. 42, Is. C. - P147-153. - ISSN 01661116 (ISSN) , DOI 10.1016/S0166-1116(08)70322-4
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Manukovski N.S., Teremova M.I., Gurevich Y.L., Pan'Kova I.M.
Заглавие : Phenol and Naphthalene degradation by mixed culture of microorganisms
Место публикации : Studies in Environmental Science. - 1991. - Vol. 42, Is. C. - P155-163. - ISSN 01661116 (ISSN) , DOI 10.1016/S0166-1116(08)70323-6
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Raibekas A.A.
Заглавие : Green flavoprotein from P. leiognathi: purification, characterization and identification as the product of the lux G(N) gene
Место публикации : Journal of bioluminescence and chemiluminescence. - 1991. - Vol. 6, Is. 3. - P169-176. - ISSN 08843996 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacterial protein--flavoprotein--amino acid sequence--article--bacterial gene--chemistry--genetics--isolation and purification--luminescence--molecular genetics--molecular weight--photobacterium--amino acid sequence--bacterial proteins--flavoproteins--genes, bacterial--luminescence--molecular sequence data--molecular weight--photobacterium--support, u.s. gov't, p.h.s.
Аннотация: A green flavoprotein (GFP) was isolated and purified to homogeneity from Photobacterium leiognathi, strain 208. GFP is a homodimer of molecular weight 54,000 and contains two molecules of an unusual flavin per molecule of protein. Various biochemical characteristics including isoelectric point, trypsin and chymotrypsin degradation, SDS and temperature influence on subunit dissociation and the dissociation of the flavin chromophore, were investigated. The sequence of 23 N-terminal amino acids was determined and found to be concurrent with the N-terminal amino acid sequence encoded by the lux G(N) gene of P. leiognathi. This fact suggests that GFP is a structural component of the Photobacterium luminescence system.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : SHALAEVA E.E., LISOVSKII G.M., TIKHOMIROV A.A.
Заглавие : SPECTRAL COMPOSITION OF LIGHT AND SOME CHARACTERISTICS OF LEAF MESOSTRUCTURE IN DIFFERENT PLANTS
Колич.характеристики :6 с
Место публикации : SOVIET PLANT PHYSIOLOGY: PLENUM PUBL CORP, 1991. - Vol. 38, Is. 1. - P40-45. - ISSN 0038-5719
Примечания : Cited References: 11
Ключевые слова (''Своб.индексиров.''): radiation spectrum--leaf mesostructure--species specificity
Аннотация: Cross sections and macerates were used to study leaf mesostructure in cucumber, sunflower, and tomato plants formed under controlled environmental conditions at an irradiance of 100 W.m-2 in the red and blue regions of the spectrum. White light served as the control. Three types of response to red light were isolated: the first type is a response not associated with harmful action of the light factor (tomato); the second a response associated with reversible changes of mesostructure (sunflower); and the third a response associated with irreversible changes of leaf mesostructure (cucumber). It is concluded that destructive changes during prolonged action of red radiation on the leaf are mainly associated with degradation of palisade parenchyma, whereas spongy parenchyma suffers less and takes upon itself the main functional role in certain species (sunflower). Species specificity was not clarified in the response of leaf mesostructure to radiation in the blue region of the spectrum.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Belyaeva O.G., Plotnikov V.F., Puzyr A.P.
Заглавие : Research of biodegradability of microbial polyhydroxyalkanoates
Место публикации : Dokl. Akad. Nauk: MEZHDUNARODNAYA KNIGA, 1996. - Vol. 350, Is. 5. - С. 707-711. - 5. - ISSN 0869-5652
Примечания : Cited References: 10
Предметные рубрики: HYDROLYTIC DEGRADATION
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sarangova A.B., Somova L.A.
Заглавие : A new enzymatic technique to estimate the efficiency of microbial degradation of pollutants
Место публикации : Advances in Space Research. - 1997. - Vol. 20, Is. 10. - С. 2049-2052. - ISSN 02731177 (ISSN)
Ключевые слова (''Своб.индексиров.''): catalase--hydrogen peroxide--aerobic metabolism--article--bacterium--biomass--bioremediation--enzymology--metabolism--methodology--microbiology--sewage--waste management--water management--aerobiosis--bacteria--biodegradation, environmental--biomass--catalase--hydrogen peroxide--sewage--waste management--water microbiology--water purification
Аннотация: Dynamics of active sludge microorganism activity in aerotanks under chemostat conditions has been studied. Dependence of microorganism catalase activity has been found to depend on residual substrate concentration in proportion to the biomass of microorganisms. Experimental data and field observations has formed the basis to develop a technique to evaluate in relative units the amount of the substrate consumed by biocenosis of the active sludge in the air tanks of purification facilities. В© 1997 COSPAR. Published by Elsevier Science Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Setkov N.A.
Заглавие : Liposomal cysteine proteinases inhibitors can commitment resting NIH 3T3 cells to proliferate
Колич.характеристики :4 с
Место публикации : Dokl. Akad. Nauk: MEZHDUNARODNAYA KNIGA, 1997. - Vol. 354, Is. 3. - P405-408. - ISSN 0869-5652
Примечания : Cited References: 14
Предметные рубрики: FIBROBLASTS
GROWTH
DEGRADATION
TURNOVER
WOS
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Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Sarangova A.B., Somova L.A.
Заглавие : A new enzymatic technique to estimate the efficiency of microbial degradation of pollutants
Колич.характеристики :4 с
Место публикации : LIFE SCIENCES: LIFE SUPPORT SYSTEMS STUDIES-I. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON PRESS LTD, 1997. - Vol. 20: F4.6, F4.8, F4.2 and F4.9 Symposia of COSPAR Scientific Commission F on Life Sciences - Life Support System Studies-I, at the 31st COSPAR Scientific Assembly (JUL 14-SEP 21, 1996, BIRMINGHAM, ENGLAND), Is. 10. - P2049-2052. - ISBN 0273-1177, DOI 10.1016/S0273-1177(97)00940-X. - ISBN 0-08-043307-3
Примечания : Cited References: 4
Аннотация: Dynamics of active sludge microorganism activity in aerotanks under chemostat conditions has been studied. Dependence of microorganism catalase activity has been found to depend on residual substrate concentration in proportion to the biomass of microorganisms. Experimental data and field observations has formed the basis to develop a technique to evaluate in relative units the amount of the substrate consumed by biocenosis of the active sludge in the air tanks of purification facilities. (C) 1997 COSPAR. Published by Elsevier Science Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gurevich Y.L., Teremova M.I., Latyntseva A.V.
Заглавие : Flocculation of bacterial cells in a culture with protozoans
Колич.характеристики :5 с
Место публикации : Izv. Akad. Nauk Ser. Biol.: MEZHDUNARODNAYA KNIGA, 1998. - Is. 3. - P351-355. - ISSN 0002-3329
Примечания : Cited References: 13
Аннотация: The initial phase of formation of the biogenic suspension was studied in experimental communities of bacteria and protozoans that simulate degradation of the phenol technogenic flows. Protozoans were shown to initiate formation of bacterial aggregates and increase the size of bacterial flocules several hundred times. Factors were found by the methods of mathematical planning, which markedly affect the size and amount of flocules. Unlike natural water bodies, in the sample communites, the presence of abiogenic substrate was not essential for aggregation of the bacteria, The aggregated bacterial cells make an important contribution to degradation of organic compounds.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondar V.S., Boersma M.G., Golovlev E.L., Vervoort J..., Van Berkel WJH, Finkelstein Z.I., Solyanikova I.P., Golovleva L.A., Rietjens IMCM
Заглавие : (19)F NMR study on the biodegradation of fluorophenols by various Rhodococcus species
Колич.характеристики :12 с
Место публикации : Biodegradation: SPRINGER, 1998. - Vol. 9: NMR in Environmental Sciences Symposium (OCT 24, 1997, EDE, NETHERLANDS), Is. 6. - С. 475-486. - ISSN 0923-9820, DOI 10.1023/A:1008391906885
Примечания : Cited References: 37
Предметные рубрики: NUCLEAR-MAGNETIC-RESONANCE
PSEUDOMONAS-PUTIDA MT-2
PHENOL HYDROXYLASE
BACTERIAL-DEGRADATION
3-OXOADIPATE PATHWAY
FLUOROBENZOIC ACID
CONVERSION
METABOLISM
CYCLOISOMERASES
2-CHLORO-CIS,CIS-MUCONATE
Ключевые слова (''Своб.индексиров.''): biodegradation--fluorophenols--(19)f nmr--oxidative defluorination--rhodococcus species
Аннотация: Of all NMR observable isotopes (19)F is the one perhaps most convenient for studies on biodegradation of environmental pollutants. The reasons underlying this potential of (19)F NMR are discussed and illustrated on the basis of a study on the biodegradation of fluorophenols by four Rhodococcus strains. The results indicate marked differences between the biodegradation pathways of fluorophenols among the various Rhodococcus species. This holds not only for the level and nature of the fluorinated biodegradation pathway intermediates that accumulate, but also for the regioselectivity of the initial hydroxylation step. Several of the Rhodococcus species contain a phenol hydroxylase that catalyses the oxidative defluorination of ortho-fluorinated di- and trifluorophenols. Furthermore, it is illustrated how the (19)F NMR technique can be used as a tool in the process of identification of an accumulated unknown metabolite, in this case most likely 5-fluoromaleylacetate. Altogether, the (19)F NMR technique proved valid to obtain detailed information on the microbial biodegradation pathways of fluorinated organics, but also to provide information on the specificity of enzymes generally considered unstable and, for this reason, not much studied so far.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondar V.S., Boersma M.G., van Berkel WJH, Finkelstein Z.I., Golovlev E.L., Baskunov B.P., Vervoort J..., Golovleva L.A., Rietjens IMCM
Заглавие : Preferential oxidative dehalogenation upon conversion of 2-halophenols by Rhodococcus opacus 1G
Колич.характеристики :10 с
Место публикации : FEMS Microbiol. Lett.: ELSEVIER SCIENCE BV, 1999. - Vol. 181, Is. 1. - С. 73-82. - ISSN 0378-1097, DOI 10.1111/j.1574-6968.1999.tb08828.x
Примечания : Cited References: 30
Предметные рубрики: PHENOL HYDROXYLASE
POLYCHLORINATED PHENOLS
MICROBIAL-DEGRADATION
TRICHOSPORON-CUTANEUM
METABOLISM
PURIFICATION
REGIOSELECTIVITY
CHLOROAROMATICS
COMPONENT
BENZOATE
Ключевые слова (''Своб.индексиров.''): phenol hydroxylase--oxidative dehalogenation--2-halophenol--f-19 nmr--rhodococcus opacus
Аннотация: The regiospecificity of hydroxylation of C2-halogenated phenols by Rhodococcus opacus 1G was investigated. Oxidative defluorination at the C2 position ortho with respect to the hydroxyl moiety was preferred over hydroxylation at the non fluorinated C6 position for all 2-fluorophenol compounds studied. Initial hydroxylation of 2,3,5-trichlorophenol resulted in the exclusive formation of 3,5-dichlorocatechol. These results indicate that, in contrast to all other phenol ortho-hydroxylases studied so far, phenol hydroxylase from R. opacus 1G is capable of catalyzing preferential oxidative defluorination but also oxidative dechlorination. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Trusova M.Y., Tyul'kova N.A., Puzyr' A.P.
Заглавие : Intracellular degradation of poly-beta-hydroxybutyrate in the hydrogen-oxidizing bacterium Alcaligenes eutrophus
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1999. - Vol. 68, Is. 1. - С. 52-57. - 6. - ISSN 0026-2617
Примечания : Cited References: 16
Предметные рубрики: METABOLISM
Ключевые слова (''Своб.индексиров.''): hydrogen-oxidizing bacteria--poly-beta-hydroxybutyrate--degradation--cell size
Аннотация: The intracellular degradation of poly-beta-hydroxybutyrate (PHB) in Alcaligenes eutrophus culture was found to occur at different: rates, depending on growth conditions, The degradation of PHB probably allows protein. synthesis and cell growth under nonoptimal environmental conditions. The isolated native granules of poly-beta-hydroxybutyrate were stable, unless they were isolated from biomass subjected to freezing-thawing treatment. Degradation of isolated granules could also be stimulated by increasing the ionic strength of the medium or-adding cell-free extracts, It is anticipated that bacterial cells contain soluble factors that either stimulate or inhibit the degradation of PHB granules.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Yu Trusova M., Tyul'Kova N.A., Puzyr' A.P.
Заглавие : Intracellular degradation of poly-?-hydroxybutyrate in the hydrogen-oxidizing bacterium alcaligenes eutrophus
Место публикации : Mikrobiologiya. - 1999. - Vol. 68, Is. 1. - С. 63-69. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): cell dimensions--degradation--hydrogen-oxidizing bacteria--poly-?-hydroxybutyrate
Аннотация: The intracellular degradation of poly-?-hydroxybutyrate (PHB) in Alcaligenes eutrophus culture was found to occur at different rates, depending on growth conditions. The degradation of PHB probably allows protein synthesis and cell growth under nonoptimal environmental conditions. The isolated native granules of poly-?-hydroxybutyrate were stable, unless they were isolated from biomass subjected to freezing-thawing treatment. Degradation of isolated granules could also be stimulated by increasing the ionic strength of the medium or adding cell-free extracts. It is anticipated that bacterial cells contain soluble factors that either stimulate or inhibit the degradation of PHB granules.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Lesniak D.V., Popova L.I.
Заглавие : Conflict: induction-inhibition of transgene bacteria luminescence in studying expression of lux-genes
Место публикации : Biofizika. - 2002. - Vol. 47, Is. 6. - P1059-1063. - ISSN 00063029 (ISSN)
Ключевые слова (''Своб.индексиров.''): naphthalene derivative--salicylic acid derivative--article--bacterial gene--chemistry--chemoluminescence--culture medium--escherichia coli--gene expression regulation--genetics--metabolism--pseudomonas fluorescens--transgene--chemiluminescent measurements--culture media--escherichia coli--gene expression regulation, bacterial--genes, bacterial--naphthalenes--pseudomonas fluorescens--salicylates--transgenes
Аннотация: The relationship between the induction of the luminescent operon of lux-genes fused with the naphthalene and salicylate degradation genes and the inhibition of light emission caused by these compounds was studied. The quantitative correlations between these processes manifest themselves in the fact that light intensity linearly increased in a narrow concentration range of the inductor and then decreased due to the inhibition of the luminescence reaction itself, which is not related to the regulation of expression of lux-genes.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Lesnyak D.V., Popova L.Y.
Заглавие : A conflict: Induction-inhibition of luminescence in the expression of lux-genes in transgenic bacteria
Колич.характеристики :5 с
Место публикации : Biofizika: MEZHDUNARODNAYA KNIGA, 2002. - Vol. 47, Is. 6. - P1059-1063. - ISSN 0006-3029
Примечания : Cited References: 12
Ключевые слова (''Своб.индексиров.''): salicylate, naphtalene concentration--bacterial bioluminescence, biodegradation
Аннотация: The relationship between the induction of the luminescent operon of lux-genes fused with the naphthalene and salicylate degradation genes and the inhibition of light emission caused by these compounds was studied. The quantitative correlations between these processes manifest themselves in the fact that light intensity linearly increased in a narrow concentration range of the inductor and then decreased due to the inhibition of the luminescence reaction itself, which is not related to the regulation of expression of lux-genes.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G.
Заглавие : A comparative investigation of biodegradable polyhydroxyalkanoate films as matrices for in vitro cell cultures
Место публикации : J. Mater. Sci.-Mater. Med.: KLUWER ACADEMIC PUBL, 2004. - Vol. 15, Is. 8. - С. 915-923. - 9. - ISSN 0957-4530, DOI 10.1023/B:JMSM.0000036280.98763.c1
Примечания : Cited References: 34
Предметные рубрики: DEGRADATION
POLY(3-HYDROXYBUTYRATE)
POLYESTERS
POLYMERS
Аннотация: The paper describes the production and investigation of flexible films made of high-purity polyhydroxyalkanoates (PHAs) - polyhydroxybutyrate [poly-(3HB)] and poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate [poly(3Hl3-co-3HV)], containing 4-30 mol % hydroxyvalerate. Poly(3HB-co-3HV) films have a more porous structure than poly-(3HB) films, which are more compact, but their surface properties, such as wettability and surface and interface energies, are the same. Sterilisation of the PHA films by conventional methods (heat treatment and gamma-irradiation) did not impair their strength. Cells cultured on PHA films exhibited high levels of cell adhesion. Cell morphology, protein synthesis and DNA synthesis were estimated by extent of H-3-thymidine incorporation into the animal cell cultures of various origins (fibroblasts, endothelium cells, and isolated hepatocytes) in direct contact with PHAs. The investigation showed that this material can be used to make matrices for in vitro proliferous cells. The investigated properties of poly-(3HB) and poly(3HB-co-3HV) films proved to be fundamentally similar. (C) 2004 Kluwer Academic Publishers.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G.
Заглавие : A comparative investigation of biodegradable polyhydroxyalkanoate films as matrices for in vitro cell cultures
Колич.характеристики :9 с
Место публикации : J. Mater. Sci.-Mater. Med.: KLUWER ACADEMIC PUBL, 2004. - Vol. 15, Is. 8. - P915-923. - ISSN 0957-4530, DOI 10.1023/B:JMSM.0000036280.98763.c1
Примечания : Cited References: 34
Предметные рубрики: DEGRADATION
POLY(3-HYDROXYBUTYRATE)
POLYESTERS
POLYMERS
Аннотация: The paper describes the production and investigation of flexible films made of high-purity polyhydroxyalkanoates (PHAs) - polyhydroxybutyrate [poly-(3HB)] and poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate [poly(3Hl3-co-3HV)], containing 4-30 mol % hydroxyvalerate. Poly(3HB-co-3HV) films have a more porous structure than poly-(3HB) films, which are more compact, but their surface properties, such as wettability and surface and interface energies, are the same. Sterilisation of the PHA films by conventional methods (heat treatment and gamma-irradiation) did not impair their strength. Cells cultured on PHA films exhibited high levels of cell adhesion. Cell morphology, protein synthesis and DNA synthesis were estimated by extent of H-3-thymidine incorporation into the animal cell cultures of various origins (fibroblasts, endothelium cells, and isolated hepatocytes) in direct contact with PHAs. The investigation showed that this material can be used to make matrices for in vitro proliferous cells. The investigated properties of poly-(3HB) and poly(3HB-co-3HV) films proved to be fundamentally similar. (C) 2004 Kluwer Academic Publishers.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Plotnikov V.F., Shishatskaya E.I., Mironov P.V., Vasil'ev A.D.
Заглавие : Physicochemical properties of two-component polyhydroxyalkanoates [Poly(3HB/3HV)]
Колич.характеристики :9 с
Место публикации : Biofizika: MEZHDUNARODNAYA KNIGA, 2004. - Vol. 49, Is. 6. - P1038-1046. - ISSN 0006-3029
Примечания : Cited References: 21
Предметные рубрики: BACTERIAL POLYHYDROXYALKANOATES
POLYESTERS
Ключевые слова (''Своб.индексиров.''): polyhydroxyalkanoates--copolymers of hydroxybutyrate and hydroxyvalerate--structure--physicochemical properties
Аннотация: A series of two-component polyhydroxyalkanoates consisting of hydroxybutyrate and hydroxyvalerate monomer at different ratios were synthesized using the bacterium Ralstonia eutropha B5786. The properties of polyhydroxyalkanoates were compared with those of the homopolymer of hydroxybutyric acid by X-ray structure analysis, IR spectroscopy, differential scanning calorimetry, and viscosimetry. With an increase in the molar fraction of hydroxyvalerate, an equalization of the ratio of the crystalline and amorphous phases in the copolymer was observed. The degree of crystallinity of the polymer decreased from 70-80 to 45-50%; in the range of an increase in the hydroxyvalerate molar fraction from several to 25-30 mol %, the dependence was linear. The temperature characteristics, the melting temperature (T-m), and the degradation temperature (T-d) were lower in polyhydroxyalkanoates than in polyhydroxybutyrate, for which T-m and T-d were 168-170 and 260-265degreesC, respectively. In the copolymer, as the molar fraction of hydroxyvalerate grew, both parameters decreased. In the range of variation of monomer ratio studied, they decreased to 150-160 and 200-220degreesC, respectively. No distinct correlation between the composition of the polymer and its molecular mass was found.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Gorbunova O.V., Zhila N.O.
Заглавие : Dynamics of activity of the key enzymes of polyhydroxyalkanoate metabolism in Ralstonia eutropha B5786
Колич.характеристики :8 с
Место публикации : Appl. Biochem. Microbiol.: MAIK NAUKA/INTERPERIODICA, 2004. - Vol. 40, Is. 2. - P170-177. - ISSN 0003-6838, DOI 10.1023/B:ABIM.0000018921.04863.d5
Примечания : Cited References: 27
Предметные рубрики: POLY-BETA-HYDROXYBUTYRATE
ORGANISM ALCALIGENES-EUTROPHUS
ESCHERICHIA-COLI
PHB
POLY(3-HYDROXYBUTYRATE)
BIOSYNTHESIS
CLONING
GENES
CHAIN
H16
Аннотация: The dynamics of accumulation of polyhydroxybutyrate (PHB) and the activities of key enzymes of PHB metabolism (beta-ketothiolase, acetoacetyl-CoA reductase, PHB synthase, D-hydroxybutyrate dehydrogenase, and PHB depolymerase) in the hydrogen bacterium Ralstonia eutropha B5786 were studied under various conditions of carbon nutrition and substrate availability. The highest activities of beta-ketothiolase, acetoacetyl-CoA reductase, and PHB synthase were recorded during acceleration of PHB synthesis. The activities of enzymes catalyzing PHB depolymerization (PHB depolymerase and D-hydroxybutyrate dehydrogenase) were low, being expressed only upon stimulated endogenous PHB degradation. The change of carbon source (CO2 or fructose) did not affect the time course of the enzyme activity significantly.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Puzyr A.P., Mogilnaya O.A., Efremov S.N.
Заглавие : Tissue response to the implantation of biodegradable polyhydroxyalkanoate sutures
Колич.характеристики :10 с
Место публикации : J. Mater. Sci.-Mater. Med.: KLUWER ACADEMIC PUBL, 2004. - Vol. 15, Is. 6. - P719-728. - ISSN 0957-4530, DOI 10.1023/B:JMSM.0000030215.49991.0d
Примечания : Cited References: 34
Предметные рубрики: DEGRADATION
POLYESTERS
POLYMERS
FIBERS
PHB
Аннотация: Polyhydroxyalkanoate (PHA) sutures were implanted to test animals intramuscularly, and tissue reaction was investigated and compared with the reaction to silk and catgut. Tested monofilament sutures made of PHAs of two types-polyhydroxybutyrate (PHB) and a copolymer of hydroxybutyrate and hydroxyvalerate (PHV)-featured the strength necessary for the healing of muscle-fascial wounds. The reaction of tissues to polymeric implants was similar to their reaction to silk and was less pronounced than the reaction to catgut; it was expressed in a transient post-traumatic inflammation (up to four weeks) and the formation of a fibrous capsule less than 200 mum thick, which became as thin as 40-60 mum after 16 weeks, in the course of reverse development. Macrophages and foreign-body giant cells with a high activity of acid phosphatase were actively involved in this process. PHB and PHB/PHV sutures implanted intramuscularly for an extended period (up to one year) did not cause any acute vascular reaction at the site of implantation or any adverse events, such as suppurative inflammation, necrosis, calcification of the fibrous capsule or malignant tumor formation. No statistically significant differences were revealed in the tissue response to polymer sutures of the two types. Capsules around silk and catgut sutures did not become significantly thinner. (C) 2004 Kluwer Academic Publishers.
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