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Variation of Spectral Characteristics of Coelenteramide-Containing Fluorescent Protein from Obelia Longissima Exposed to Dimethyl Sulfoxide / A. S. Petrova [et al.] // Russ. Phys. J. - 2016. - Vol. 59, Is. 4. - P562-567, DOI 10.1007/s11182-016-0806-8. - Cited References:33. - This work was supported in part by the Russian Science Foundation (Contract No. 14-14-00076). . - ISSN 1064-8887. - ISSN 1573-9228

РУБ Physics, Multidisciplinary
Рубрики:
CA2+-REGULATED PHOTOPROTEINS
SPECTROSCOPIC PROPERTIES
Кл.слова (ненормированные):
fluorescent coelenteramide-containing fluorescent proteins -- discharged -- obelin -- proton transfer -- dimethyl sulfoxide
Аннотация: Effect of dimethyl sulfoxide (DMSO), a widespread biomedical agent, on spectral-luminescent characteristics of coelenteramide-containing fluorescent protein - discharged obelin - is investigated. Contributions of violet and blue-green spectral components to fluorescence of discharged obelin are elucidated and characterized at different photoexcitation energies. Dependences of these contributions on the DMSO concentration are presented. Spectral changes are related to the destructive effect of DMSO on fluorescent protein and decreasing efficiency of proton transfer to electronically excited states of fluorophore.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.
Krasnoyarsk State Agrarian Univ, Krasnoyarsk, Russia.

Доп.точки доступа:
Petrova, A. S.; Alieva, R. R.; Belogurova, N. V.; Tirranen, L. S.; Kudryasheva, N. S.; Russian Science Foundation [14-14-00076]

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Usage of different neural networks in identification of plant types / S. Bartsev, Y. Ivanova, M. Saltykov // IOP Conference Series: Materials Science and Engineering : Institute of Physics Publishing, 2020. - Vol. 734: 2nd International Scientific Conference on Advanced Technologies in Aerospace, Mechanical and Automation Engineering, MIST: Aerospace 2019 (18 November 2019 through 21 November 2019, ) Conference code: 157461, Is. 1. - Ст. 012097, DOI 10.1088/1757-899X/734/1/012097
Аннотация: Since introduction of neural networks into remote sensing they demonstrate good efficiency in remote sensing data analysis. This work is devoted to processing of multispectral (12 bands) images from Sentinel-2(A, B) satellites. Satellite images of areas in Krasnoyarsk Region and Khakassia with known vegetation types are used as task books to train neural networks. Trained neural networks have been reduced to determine which bands are significant for vegetation type identification. Reduction of trained neural network show that vegetation type can be determined from only four infrared bands without significant loses in performance in comparison with non-reduced neural network. © Published under licence by IOP Publishing Ltd.

Scopus
Держатели документа:
Institute of Biophysics FRC KSC SB RAS, Akademgorodok 50/50, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Bartsev, S.; Ivanova, Y.; Saltykov, M.

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Transfer efficiency of carbon, nutrients, and polyunsaturated fatty acids in planktonic food webs under different environmental conditions / M. Karpowicz, I. Feniova, M. I. Gladyshev [et al.] // Ecology and Evolution. - 2021, DOI 10.1002/ece3.7651 . - Article in press. - ISSN 2045-7758
Кл.слова (ненормированные):
biogeochemical cycle -- dystrophication -- essential substances -- eutrophication -- food quality -- phytoplankton -- zooplankton
Аннотация: The trophic transfer efficiency (TTE) is an important indicator of ecosystem functioning. However, TTE data from freshwater food webs are ambiguous due to differences in time scales and methods. We investigated the transfer of essential substances (carbon, nutrients, and polyunsaturated fatty acids) through plankton communities in 30 Polish lakes with different trophic status in the middle of summer. The results of our study revealed that different essential substances were transferred from phytoplankton to zooplankton with varying efficiencies. The average TTE of C, N, P, and the sum of ?-3 PUFA were 6.55%, 9.82%, 15.82%, and 20.90%, respectively. Our results also show a large mismatch between the elemental and biochemical compositions of zooplankton and their food during the peak of the summer stagnation, which may further promote the accumulation of essential substances. There were also large differences in TTEs between trophic conditions, with the highest efficiencies in oligotrophic lakes and the lowest in dystrophic and eutrophic lakes. Therefore, our study indicates that disturbances like eutrophication and dystrophication similarly decrease the TTE of essential substances between phytoplankton and zooplankton in freshwater food webs. © 2021 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.

Scopus
Держатели документа:
Department of Hydrobiology, Faculty of Biology, University of Bialystok, Bialystok, Poland
Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, Russian Federation
Institute of Biophysics of Federal Research Centre, Krasnoyarsk Science Centre of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Research Station in Mikolajki, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland
Department of Integrative Biology, Oklahoma State University, Stillwater, OK, United States

Доп.точки доступа:
Karpowicz, M.; Feniova, I.; Gladyshev, M. I.; Ejsmont-Karabin, J.; Gorniak, A.; Sushchik, N. N.; Anishchenko, O. V.; Dzialowski, A. R.

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Transfer efficiency of carbon, nutrients, and polyunsaturated fatty acids in planktonic food webs under different environmental conditions / M. Karpowicz, I. Feniova, M. I. Gladyshev [et al.] // Ecol. Evol. - 2021, DOI 10.1002/ece3.7651. - Cited References:62. - This research was supported by the Polish National Science Centre (2016/21/B/NZ8/00434). The research was also supported by Federal Tasks for Institute of Biophysics SB RAS No. 51.1.1 and Federal Tasks for Siberian Federal University No. FSRG-2020-0019. The authors are thankful to Joanna Kozowska for her help in the collection of samples. . - Article in press. - ISSN 2045-7758

РУБ Ecology + Evolutionary Biology
Рубрики:
PHOSPHORUS STOICHIOMETRY
   LIGHT-INTENSITY
   ZOOPLANKTON
   TEMPERATURE
Кл.слова (ненормированные):
biogeochemical cycle -- dystrophication -- essential substances -- eutrophication -- food quality -- phytoplankton -- zooplankton
Аннотация: The trophic transfer efficiency (TTE) is an important indicator of ecosystem functioning. However, TTE data from freshwater food webs are ambiguous due to differences in time scales and methods. We investigated the transfer of essential substances (carbon, nutrients, and polyunsaturated fatty acids) through plankton communities in 30 Polish lakes with different trophic status in the middle of summer. The results of our study revealed that different essential substances were transferred from phytoplankton to zooplankton with varying efficiencies. The average TTE of C, N, P, and the sum of omega-3 PUFA were 6.55%, 9.82%, 15.82%, and 20.90%, respectively. Our results also show a large mismatch between the elemental and biochemical compositions of zooplankton and their food during the peak of the summer stagnation, which may further promote the accumulation of essential substances. There were also large differences in TTEs between trophic conditions, with the highest efficiencies in oligotrophic lakes and the lowest in dystrophic and eutrophic lakes. Therefore, our study indicates that disturbances like eutrophication and dystrophication similarly decrease the TTE of essential substances between phytoplankton and zooplankton in freshwater food webs.

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Держатели документа:
Univ Bialystok, Dept Hydrobiol, Fac Biol, Ciolkowskiego 1J, PL-15245 Bialystok, Poland.
Russian Acad Sci, Inst Ecol & Evolut, Moscow, Russia.
Russian Acad Sci, Krasnoyarsk Sci Ctr, Siberian Branch, Inst Biophys,Fed Res Ctr, Krasnoyarsk, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.
Polish Acad Sci, Nencki Inst Expt Biol, Res Stn Mikolajki, Warsaw, Poland.
Oklahoma State Univ, Dept Integrat Biol, Stillwater, OK 74078 USA.

Доп.точки доступа:
Karpowicz, Maciej; Feniova, Irina; Gladyshev, Michail I.; Ejsmont-Karabin, Jolanta; Gorniak, Andrzej; Sushchik, Nadezhda N.; Anishchenko, Olesya V.; Dzialowski, Andrew R.; Polish National Science Centre [2016/21/B/NZ8/00434]; Federal Tasks for Institute of Biophysics SB RAS [51.1.1]; Federal Tasks for Siberian Federal University [FSRG-2020-0019]

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The Sensitivity of Resting Eggs of the Cladoceran Moina macrocopa to the Effect of Ionizing Radiation during the Reactivation of the Eggs / T. S. Lopatina [et al.] // Dokl. Biochem. Biophys. - 2018. - Vol. 480, Is. 1. - P169-172, DOI 10.1134/S1607672918030122. - Cited References:10. - The study was supported by the Russian Foundation for Basic Research (project no. 15-04-05199), and the joint grant of the Russian Foundation for Basic Research, the Krasnoyarsk Regional Foundation for Support of Science and Technology (project no. 16-44-243041). . - ISSN 1607-6729. - ISSN 1608-3091

РУБ Biochemistry & Molecular Biology + Biophysics

Аннотация: We investigated the sensitivity of resting eggs of the cladoceran Moina macrocopa to the effect of ionizing radiation during the reactivation of the eggs. The study showed that the resting eggs during reactivation are more vulnerable to irradiation than the resting eggs in a stage of deep dormancy. The decrease in the efficiency of egg reactivation was observed at high doses, the growth rate of juveniles, fecundity, and the number of produced clutches by females strongly decreased when resting eggs at the reactivation stage absorbed doses of 64 Gy and higher.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Krasnoyarsk Res Ctr, Inst Biophys, Krasnoyarsk 630036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
Russian Acad Sci, Siberian Branch, Budker Inst Nucl Phys, Novosibirsk 630090, Russia.

Доп.точки доступа:
Lopatina, T. S.; Zadereev, E. S.; Oskina, N. A.; Petrichenkov, M. V.; Russian Foundation for Basic Research [15-04-05199, 16-44-243041]; Krasnoyarsk Regional Foundation [16-44-243041]

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The role of population density in gamogenesis induction in moina macrocopa (Cladocera: Crustaceae) / E. S. Zadereev, V. G. Gubanov // Zhurnal Obshchei Biologii. - 1996. - Vol. 57, Is. 3. - С. 366-367 . - ISSN 0044-4596
Аннотация: Combined influence of population density, temperature and photoperiod on the gamogenesis of individual females of M. macrocopa was investigated. In all experiments the influence of population density was associated with the direct effect of "swarm water". The photoperiod in its broad natural range also influences gamogenesis induction. The increase in temperature above optimum but lower than lethal level sharply decreases the efficiency of gamogenesis. In this case stimulation of gamogenesis is possible by combined effect of poor feeding and high population density. If temperature and photoperiod are optimum for partenogenetic population the change of reproduction mode in lowdensity population of M. macrocopa is determined by poor feeding while in high-density populations density itself is more important. However the increase in food concentration descreases the efficiency of "swarm water" on the gamogenesis induction. According to the hypothesis of nondirect influence of population density an individual decreases its filtarion rate (and starves) under "swarm water" condition.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Br. Russ. Acad. of Science, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zadereev, E.S.; Gubanov, V.G.

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The levels of organization of the photosynthetic apparatus and the control of production processes in phytocenoses under artificial-light culture / A. A. Tikhomirov, G. M. Lisovskii // Russian Journal of Plant Physiology. - 2001. - Vol. 48, Is. 3. - P395-399, DOI 10.1023/A:1016682904411 . - ISSN 1021-4437
Кл.слова (ненормированные):
Artificial-light culture -- Photosynthetic apparatus -- Phytocenosis -- Productivity -- Embryophyta
Аннотация: The processes limiting the production in higher plant phytocenoses under an artificial-light culture are analyzed in relation to the multilevel organization of the photosynthetic apparatus (PA). The authors consider the feasibility of overcoming these limitations by optimizing the physical parameters of irradiation (the structure of the light spectrum, the rate, and the ratio of radiation fluxes in photosynthetically active radiation (PAR) and infrared (IR) regions) at the molecular, leaf, plant, and cenotic levels of PA organization. To illustrate this approach, the authors used a complex experiment in an artificial ecosystem to evaluate the efficiency of the light control of production processes in multispecies phytocenoses by alleviating or removing the factors that limit plant production at the various levels of PA organization. An artificial-light culture is seen as an instrument for solving several problems of theoretical and applied plant physiology and related disciplines in the future.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Lisovskii, G.M.

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The levels of organization of the photosynthetic apparatus and the control of production processes in phytocenoses under artificial-light culture [Text] / A. A. Tikhomirov, G. M. Lisovskii // Russ. J. Plant Physiol. - 2001. - Vol. 48, Is. 3. - P. 395-399, DOI 10.1023/A:1016682904411. - Cited References: 21 . - ISSN 1021-4437

РУБ Plant Sciences

Кл.слова (ненормированные):
photosynthetic apparatus -- phytocenosis -- productivity -- artificial-light culture
Аннотация: The processes limiting the production in higher plant phytocenoses under an artificial-light culture are analyzed in relation to the multilevel organization of the photosynthetic apparatus (PA). The authors consider the feasibility of overcoming these limitations by optimizing the physical parameters of irradiation (the structure of the light spectrum, the rate, and the ratio of radiation fluxes in photosynthetically active radiation (PAR) and infrared (IR) regions) at the molecular, leaf, plant, and cenotic levels of PA organization. To illustrate this approach, the authors used a complex experiment in an artificial ecosystem to evaluate the efficiency of the light control of production processes in multispecies phytocenoses by alleviating or removing the factors that limit plant production at the various levels of PA organization. An artificial-light culture is seen as an instrument for solving several problems of theoretical and applied plant physiology and related disciplines in the future.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Lisovskii, G.M.

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The effect of salinity on the grazing rate and survival of Daphnia magna females adapted to different salinities / E. S. Zadereev, T. S. Lopatina, S. D. Ovchinnikov [et al.] // Aquat. Ecol. - 2022, DOI 10.1007/s10452-021-09941-7. - Cited References:47. - The reported study was funded by the Krasnoyarsk Regional Fund for supporting scientific and technical activities, the Krasnoyarsk Krai Government and the Russian Foundation for Basic Research, project number 19-44-240010. We are grateful to professional English translator Elena Krasova for language corrections. . - Article in press. - ISSN 1386-2588. - ISSN 1573-5125

РУБ Ecology + Limnology + Marine & Freshwater Biology
Рубрики:
FEEDING-BEHAVIOR
PHYTOPLANKTON CONTROL
SPECIES COMPOSITION
Кл.слова (ненормированные):
Salinity -- Grazing -- Phytoplankton -- Adaptation -- Daphnia
Аннотация: The cladoceran Daphnia magna inhabits lakes with salinities up to 10 g L-1. We compared the effects of different salinities (up to 9 g L-1) on the survival, specific grazing rate, and size selective feeding of Daphnia females adapted to fresh or saline waters (3-4 g L-1). The freshwater population was more sensitive to high salinity (LC50 = 5.3 g L-1), while the survival of the saline water population also decreased in fresh water. Freshwater population demonstrated a higher grazing rate in fresh water, while the saline water population had a higher grazing rate at the salinity above 3 g L-1. A decrease in the grazing rate of the freshwater population was observed at the salinity above 4-5 g L-1. Populations differed in food selectivity. The saline water population consumed particles of larger sizes than the freshwater population. The average size of phytoplankton particles grazed in fresh water was larger than in saline water. This size selective salinity-dependent grazing may be related to the dependence of the feeding efficiency of cladocerans on the viscosity of water and size of phytoplankton particles. Our results indicate that Daphnia populations adapted to a certain salinity can temporarily lose the ability to control phytoplankton because of salinity fluctuations.

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Держатели документа:
Russian Acad Sci, Fed Res Ctr, Krasnoyarsk Sci Ctr, Inst Biophys, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, 79 Svobodniy Ave, Krasnoyarsk 660041, Russia.
Khakassky State Nat Reserve, POB 189, Abakan 655017, Russia.

Доп.точки доступа:
Zadereev, E. S.; Lopatina, T. S.; Ovchinnikov, S. D.; Oskina, N. A.; Drobotov, A., V; Tolomeev, A. P.; Krasnoyarsk Regional Fund; Krasnoyarsk Krai Government; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [19-44-240010]

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10.

The Effect of Osmolytes on the Bioluminescent Reaction of Bacteria: Structural and Dynamic Properties / L. A. Sukovatyi, A. E. Lisitsa, V. A. Kratasyuk, E. V. Nemtseva // Biophysics. - 2020. - Vol. 65, Is. 6. - P966-971, DOI 10.1134/S0006350920060202 . - ISSN 0006-3509
Кл.слова (ненормированные):
bacterial luciferase -- bioluminescence -- luminous bacteria -- molecular dynamic -- osmolyte -- protein structure and dynamics
Аннотация: The effects of viscous media with glycerol and sucrose (10–40%) on the kinetics of the bacterial bioluminescent reaction have been investigated by stopped-flow technique. Increment of quantum yield in media with 10% of both osmolytes was shown. Higher concentrations of glycerol, up to 30–40%, were found to reduce the efficiency of the reaction, while this effect was not observed in the media with sucrose. The molecular dynamics simulation was used to study the structure of bacterial luciferase surrounded by either water molecules solely or by mixture of water with various numbers of glycerol/sucrose molecules. It was found that both cosolvents at studied concentrations did not cause a significant change in conformation of bacterial luciferase. The calculated root-mean-square fluctuation for C?-atoms of bacterial luciferase ?-subunit indicated that the higher flexibility of the enzyme mobile loop could be responsible for increment of quantum yield in the presence of 10% of both osmolytes. The active site of bacterial luciferase was found to be accessible for glycerol molecules while sucrose did not enter catalytic gorge. Moreover, at 30 and 40% concentration the glycerol molecules were found to locate in the active site of bacterial luciferase throughout the whole simulation time. © 2020, Pleiades Publishing, Inc.

Scopus
Держатели документа:
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics SB RAS, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Sukovatyi, L. A.; Lisitsa, A. E.; Kratasyuk, V. A.; Nemtseva, E. V.

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11.

The analysis of seasonal activity of photosynthesis and efficiency of various vegetative communities on a basis NDVI for modeling of biosphere processes / Yu. D. Ivanova [et al.] // Advances in Space Research. - 2007. - Vol. 39, Is. 1. - P95-99, DOI 10.1016/j.asr.2006.02.028 . - ISSN 0273-1177
Кл.слова (ненормированные):
NDVI -- Plant communities -- Synoptic parameters -- Biomass -- Climatology -- Parameter estimation -- Photosynthesis -- Plants (botany) -- Statistical methods -- Time series analysis -- NDVI -- Plant communities -- Seasonal activity -- Synoptic parameters -- Vegetation
Аннотация: NDVI (Normalized Difference Vegetation Index) is proposed as an area-dependent climatic variable, which reflects climatically significant events and processes. NDVI is taken as a simple quantitative indicator of the amount of photosynthetically active biomass. Mean values of NDVI have been calculated for the period between 1996 and 2001. NDVI time series have been analyzed in conjunction with meaningful synoptic parameters that influence the behavior of plants in different plant communities of Eastern Siberia (tundra, taiga, and steppe). Based on GIS technologies, statistical tests have been carried out and correlations between the study parameters have been found. В© 2007.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Krasnoyarsk State Technical University, Kirensky 26, Krasnoyarsk, 660074, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Ivanova, Yu.D.; Bartsev, S.I.; Pochekutov, A.A.; Kartushinsky, A.V.

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12.

Synthesis of poly(3-hydroxybutyrate) by the autotrophic CO-oxidizing bacterium Seliberia carboxydohydrogena Z-1062 [Text] / T. Volova, N. Zhila, E. Shishatskaya // J. Ind. Microbiol. Biotechnol. - 2015. - Vol. 42, Is. 10. - P1377-1387, DOI 10.1007/s10295-015-1659-9. - Cited References:36. - The research was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences (project No 01201351505). . - ISSN 1367-5435. - ISSN 1476-5535

РУБ Biotechnology & Applied Microbiology
Рубрики:
CARBON-MONOXIDE
   RALSTONIA-EUTROPHA
   POLYHYDROXYALKANOATES
   GROWTH
   H-2
Кл.слова (ненормированные):
Carboxydobacteria -- Synthesis -- Poly(3-hydroxybutyrate) -- Carbon monoxide
Аннотация: The present study addresses growth parameters and physiological and biochemical characteristics of the aerobic CO-oxidizing carboxydobacterium Seliberia carboxydohydrogena Z-1062. Poly(3-hydroxybutyrate) yields were investigated in experiments with limiting concentrations of mineral nutrients (nitrogen or sulfur or nitrogen and sulfur) in batch culture of S. carboxydohydrogena Z-1062 grown on gas mixtures consisting of CO2, O-2, H-2, and CO. CO concentrations of 10, 20, and 30 % v/v did not affect polymer synthesis, whose content after 56-h cultivation under limiting concentrations of nitrogen and sulfur was 52.6-62.8 % of biomass weight at a productivity of 0.13-0.22 g/L h. The inhibitory effect of CO on cell concentration was revealed at CO concentration of 30 % v/v. That also caused a decrease in substrate (H-2 and O-2) use efficiency. Thus, this carboxydobacterium can be regarded as a potential producer of polyhydroxyalkanoates from industrial hydrogenous sources.

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Держатели документа:
RAS, SB, Inst Biophys, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Volova, Tatiana; Zhila, Natalia; Shishatskaya, Ekaterina; Russian Academy of Sciences [01201351505]

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13.

Study of the efficiency of doxorubicin deposited in microparticles from resorbable bioplastotaneв„ў on laboratory animals with Ehrlich's solid carcinoma / E. I. Shishatskaya, A. V. Goreva, A. M. Kuzmina // Bulletin of Experimental Biology and Medicine. - 2013. - Vol. 154, Is. 6. - P773-777, DOI 10.1007/s10517-013-2053-0 . - ISSN 0007-4888
Кл.слова (ненормированные):
Bioplastotane -- controlled drug delivery systems -- Ehrlich's carcinoma -- microparticles -- resorbable polymers -- doxorubicin -- drug carrier -- animal experiment -- animal model -- animal tissue -- antineoplastic activity -- article -- cancer inhibition -- controlled study -- drug delivery device -- drug delivery system -- drug dosage form comparison -- drug efficacy -- drug mechanism -- Ehrlich ascites tumor -- encapsulation -- leukocyte count -- mouse -- multiple cycle treatment -- nonhuman -- oncological parameters -- tumor volume -- tumor weight -- Animalia -- Mus
Аннотация: Antitumor efficiency of an experimental form of an experimental form of anthracyclin antibiotic (doxorubicin), resorbable microparticles from Bioplastotaneв„ў, was studied on laboratory mice with transplanted Ehrlich's solid carcinoma. Use of the experimental form of the cytostatic in polymeric microparticles from resorbable Bioplastotaneв„ў in animals with solid tumor led to inhibition of the cancerous process, comparable to that in response to intravenous free doxorubicin, but without negative effects on the blood system. В© 2013 Springer Science+Business Media New York.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Institute of Basic Biology and Biotechnology, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Goreva, A.V.; Kuzmina, A.M.

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14.

Software for matching standard activity enzyme biosensors for soil pollution analysis / V. A. Kratasyuk, E. M. Kolosova, O. S. Sutormin [et al.] // Sensors. - 2021. - Vol. 21, Is. 3. - Ст. 1017. - P1-10, DOI 10.3390/s21031017 . - ISSN 1424-8220
Кл.слова (ненормированные):
Bacterial luciferase -- Biosensors -- Butyrylcholinesterase -- Enzyme -- Lactic dehydrogenase -- Software -- Soil pollution -- Biosensors -- Soil pollution -- Soil surveys -- Soils -- Commercial standards -- Environmental Monitoring -- Enzyme biosensors -- Enzyme systems -- Inhibitory effect -- JavaScript programming -- Soil sample -- Toxic agents -- Enzyme activity
Аннотация: This work is dedicated to developing enzyme biosensor software to solve problems regarding soil pollution analysis. An algorithm and specialised software have been developed which stores, analyses and visualises data using JavaScript programming language. The developed software is based on matching data of 51 non-commercial standard soil samples and their inhibitory effects on three enzyme systems of varying complexity. This approach is able to identify the influence of chemical properties soil samples, without toxic agents, on enzyme biosensors. Such software may find wide use in environmental monitoring. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Scopus
Держатели документа:
Department of Biophysics, Institute of Fundamental Biology and Biotechology, Siberian Federal University, 79 Svobodny pr, Krasnoyarsk, 660041, Russian Federation
Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, Photobiology Laboratory, Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, 50/50 Akagemgorodok, Krasnoyarsk, 660036, Russian Federation
Department of High-Efficiency Calculations, Siberian Federal University, 26-ULK building Kirensky St, Krasnoyarsk, 660074, Russian Federation
Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, Krasnoyarsk Research Institute of Agriculture, Russian Academy of Sciences, Siberian Branch, 66 Svobodny pr, Krasnoyarsk, 660037, Russian Federation
Federal Research Center ‘Krasnoyarsk Scientific Center SB RAS’, Krasnoyarsk Research Institute of Agricultural, Siberian Federal University, 79 Svobodny pr, Krasnoyarsk, 660041, Russian Federation

Доп.точки доступа:
Kratasyuk, V. A.; Kolosova, E. M.; Sutormin, O. S.; Lonshakova-Mukina, V. I.; Baygin, M. M.; Rimatskaya, N. V.; Sukovataya, I. E.; Shpedt, A. A.

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15.

Software for Matching Standard Activity Enzyme Biosensors for Soil Pollution Analysis / V. A. Kratasyuk, E. M. Kolosova, O. S. Sutormin [et al.] // Sensors. - 2021. - Vol. 21, Is. 3. - Ст. 1017, DOI 10.3390/s21031017. - Cited References:20. - This research was funded by RFBR, Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science, Grant number 20-44-243001 and the Ministry of Science and Higher Education of the Russian Federation, Grant number FSRZ-2020-0006. . - ISSN 1424-8220

РУБ Chemistry, Analytical + Engineering, Electrical & Electronic + Instruments

Кл.слова (ненормированные):
biosensors -- enzyme -- butyrylcholinesterase -- lactic dehydrogenase -- bacterial luciferase -- soil pollution -- software
Аннотация: This work is dedicated to developing enzyme biosensor software to solve problems regarding soil pollution analysis. An algorithm and specialised software have been developed which stores, analyses and visualises data using JavaScript programming language. The developed software is based on matching data of 51 non-commercial standard soil samples and their inhibitory effects on three enzyme systems of varying complexity. This approach is able to identify the influence of chemical properties soil samples, without toxic agents, on enzyme biosensors. Such software may find wide use in environmental monitoring.

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Держатели документа:
Siberian Fed Univ, Inst Fundamental Biol & Biotechol, Dept Biophys, 79 Svobodny Pr, Krasnoyarsk 660041, Russia.
Russian Acad Sci, Krasnoyarsk Sci Ctr SB RAS, Photobiol Lab, Fed Res Ctr,Siberian Branch,Inst Biophys, 50-50 Akagemgorodok, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Dept High Efficiency Calculat, 26 ULK Bldg Kirensky St, Krasnoyarsk 660074, Russia.
Russian Acad Sci, Krasnoyarsk Sci Ctr SB RAS, Krasnoyarsk Res Inst Agr, Siberian Branch,Fed Res Ctr, 66 Svobodny Pr, Krasnoyarsk 660037, Russia.
Siberian Fed Univ, Inst Fundamental Biol & Biotechol, Dept Aquat & Terr Ecosyst, 79 Svobodny Pr, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Kratasyuk, Valentina A.; Kolosova, Elizaveta M.; Sutormin, Oleg S.; Lonshakova-Mukina, Viktoriya, I; Baygin, Matvey M.; Rimatskaya, Nadezhda, V; Sukovataya, Irina E.; Shpedt, Alexander A.; RFBRRussian Foundation for Basic Research (RFBR); Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science [20-44-243001]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]

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16.

Results of analysis of human impact on environment using the time series of vegetation satellite images around large industrial centers / A. Shevyrnogov [et al.] // Advances in Space Research. - 2008. - Vol. 41, Is. 1. - P36-40, DOI 10.1016/j.asr.2007.02.008 . - ISSN 0273-1177
Кл.слова (ненормированные):
Industrial wastes -- Remote sensing -- Vegetation dynamics -- Vegetation index -- Image reconstruction -- Industrial economics -- Industrial wastes -- Remote sensing -- Satellite imagery -- Time series analysis -- Vegetation dynamics -- Vegetation index -- Vegetation
Аннотация: The paper shows the efficiency of an application of the vegetation index image time series to determine long-term vegetation dynamics. The influence of large industrial centers of Siberia on the near-by vegetation is demonstrated. The analysis of the data shows that the influence of industrial waste is stronger in the Siberian North. These regions are characterized by critical conditions for vegetation existence. In the south of the Krasnoyarsk region, human impact is also important, but the possibility of vegetation self-rehabilitation is higher. The present-day economic situation in Russia is unique, with a temporary abrupt fall of industrial production and its following increase. Thus, we managed to analyze the degree of human impact on the environment within a relatively short-time interval. В© 2007.

Scopus
Держатели документа:
Institute of Biophysics of SB RAS, Krasnoyarsk, 660036 Akademgorodok, Russian Federation
Institute of Computational Modeling of SB RAS, Krasnoyarsk, 660036 Akademgorodok, Russian Federation
Institute of Forest of SB RAS, Krasnoyarsk, 660036 Akademgorodok, Russian Federation
CC Krasnoyarskgeophysics, St. Partizana Zheleznyaka, 24v, Krasnoyarsk, 660022, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shevyrnogov, A.; Vysotskaya, G.; Sukhinin, A.; Frolikova, O.; Tchernetsky, M.

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17.

Quantitative criteria for estimation of natural and artificial ecosystems functioning / N. S. Pechurkin // Advances in Space Research. - 2005. - Vol. 35, Is. 9 SPEC. ISS. - P1507-1511, DOI 10.1016/j.asr.2005.01.059 . - ISSN 0273-1177
Кл.слова (ненормированные):
Artificial ecosystems -- Limiting substances -- Natural ecosystems -- Quantitative criteria -- Carbon dioxide -- Energy management -- Estimation -- Hierarchical systems -- Personnel -- Plants (botany) -- Reliability -- Artificial ecosystems -- Limiting substances -- Natural ecosystems -- Quantitative criteria -- Ecosystems -- carbon -- biomass -- chemistry -- conference paper -- ecology -- ecosystem -- energy transfer -- microclimate -- photosynthesis -- population dynamics -- Biomass -- Carbon -- Ecological Systems, Closed -- Ecology -- Ecosystem -- Energy Transfer -- Life Support Systems -- Photosynthesis -- Population Dynamics
Аннотация: Using biotic turnover of substances in trophic chains, natural and artificial ecosystems are similar in functioning, but different in structure. It is necessary to have quantitative criteria to evaluate the efficiency of artificial ecosystems (AES). These criteria are dependent on the specific objectives for which the AES are designed. For example, if AES is considered for use in space, important criteria are efficiency in use of mass, power, volume (size) and human labor and reliability. Another task involves the determination of quantitative criteria for the functioning of natural ecosystems. To solve the problem, it is fruitful to use a hierarchical approach suitable for both individual links and the ecosystem as a whole. Energy flux criteria (principles) were developed to estimate the functional activities of biosystems at the population, community and ecosystem levels. A major feature of ecosystems as a whole is their biotic turnover of matter the rate of which is restricted by the lack of limiting substances. Obviously, the most generalized criterion is to take into account the energy flux used by the biosystem and the quantity of limiting substance included in its turnover. The use of energy flux by ecosystem, EUSED - is determined from the photoassimilation of CO2 by plants (per time unit). It can be approximately estimated as the net primary production of photosynthesis (NPP). So, the ratio of CO 2 photoassimilation rate (sometimes, measured as NPP) to the total mass of limiting substrate can serve as a main universal criterion (MUC). This MUC characterizes the specific cycling rate of limiting chemical elements in the system and effectiveness of every ecosystem including the global Biosphere. Comparative analysis and elaboration of quantitative criteria for estimation of natural and artificial ecosystems activities is of high importance both for theoretical considerations and for real applications. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, SB, RAS, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pechurkin, N.S.

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18.

Quantitative criteria for estimating the effectiveness of bioluminescence expression in natural and transgenic luminescent bacteria / A. A. Gusev [et al.] // Biophysics. - 2008. - Vol. 53, Is. 5. - P417-420, DOI 10.1134/S0006350908050175 . - ISSN 0006-3509
Кл.слова (ненормированные):
Effectiveness of lux-operon expression -- Natural and transgenic bacteria -- Bacteria (microorganisms) -- Escherichia coli -- Photobacterium leiognathi
Аннотация: Computational coefficients for estimating the effectiveness of bioluminescence expression in natural luminescent bacteria Photobacterium leiognathi 54 and transgenic strain E. coli Z905/pPHL7 bearing lux-operon in a multicopy plasmid are suggested and their use at molecular, cell, and population levels was considered. It was shown that at the population level, all transgenic variants have an advantage over natural variants of P. leiognathi 54 irrespective of the type of lux-operon regulation. At the cell level, the effectiveness of bioluminescence expression in the bright and dim variants of the transgenic strain increased by several orders. At the level of one lux-operon, the effectiveness of expression in the bright variant of the transgenic strain is substantially higher than in the natural bright variant; in dim variants, the efficiency values are similar; the effectiveness of bioluminescence expression in the dark variant of E. coli Z905-2/pPHL7 is by two orders of magnitude lower than in the dark variant of P. leiognathi 54. В© 2008 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50/50, Krasnoyarsk, 660036, Russian Federation
Krasnoyarsk Scientific Center, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gusev, A.A.; Kargatova, T.V.; Medvedeva, S.E.; Popova, L.Yu.

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19.

Protein-based fluorescent bioassay for low-dose gamma radiation exposures / A. S. Petrova [et al.] // Anal. Bioanal. Chem. - 2018, DOI 10.1007/s00216-018-1282-5 . - ISSN 1618-2642
Кл.слова (ненормированные):
Bioassay -- Enzymes -- Fluorescence/luminescence -- Fluorescent protein -- Gamma radiation -- Radiotoxicity -- Efficiency -- Enzymes -- Fluorescence -- Gamma rays -- Proteins -- Proton transfer -- Fluorescence characteristics -- Fluorescence intensities -- Fluorescence spectra -- Fluorescence/luminescence -- Fluorescent protein -- Photochemical process -- Physiological liquids -- Radiotoxicity -- Bioassay
Аннотация: The study suggests an application of a coelenteramide-containing fluorescent protein (CLM-CFP) as a simplest bioassay for gamma radiation exposures. “Discharged obelin,” a product of the bioluminescence reaction of the marine coelenterate Obelia longissima, was used as a representative of the CLM-CFP group. The bioassay is based on a simple enzymatic reaction—photochemical proton transfer in the coelenteramide-apoprotein complex. Components of this reaction differ in fluorescence color, providing, by this, an evaluation of the proton transfer efficiency in the photochemical process. This efficiency depends on the microenvironment of the coelenteramide within the protein complex, and, hence, can evaluate a destructive ability of gamma radiation. The CLM-CFP samples were exposed to gamma radiation (137Cs, 2 mGy/h) for 7 and 16 days at 20 °C and 5 °C, respectively. As a result, two fluorescence characteristics (overall fluorescence intensity and contributions of color components to the fluorescence spectra) were identified as bioassay parameters. Both parameters demonstrated high sensitivity of the CLM-CFP-based bioassay to the low-dose gamma radiation exposure (up to 100 mGy). Higher temperature (20 °C) enhanced the response of CLM-CFP to gamma radiation. This new bioassay can provide fluorescent multicolor assessment of protein destruction in cells and physiological liquids under exposure to low doses of gamma radiation. [Figure not available: see fulltext.]. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.

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Держатели документа:
Krasnoyarsk State Agrarian University, Mira Avenue 90, Krasnoyarsk, Russian Federation
Siberian Federal University, Svobodnyy Ave 79, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, FRC KSC SB RAS, Krasnoyarsk, Russian Federation
Department of Radiology, University of Pennsylvania, 3401 N Broad St., Philadelphia, PA, United States

Доп.точки доступа:
Petrova, A. S.; Lukonina, A. A.; Dementyev, D. V.; Bolsunovsky, A. Ya. ; Popov, A. V.; Kudryasheva, N. S.

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20.

Production of EPA and DHA in aquatic ecosystems and their transfer to the land / M. I. Gladyshev, N. N. Sushchik, O. N. Makhutova // Prostaglandins and Other Lipid Mediators. - 2013, DOI 10.1016/j.prostaglandins.2013.03.002 . - ISSN 1098-8823
Кл.слова (ненормированные):
Aquatic ecosystems -- Docosahexaenoic acid -- Eicosapentaenoic acid -- Trophic transfer efficiency
Аннотация: Most omnivorous animals, including humans, have to some degree relied on physiologically important polyunsaturated fatty acids (PUFAs), such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) from food. Only some taxa of microalgae, rather than higher plants can synthesize de novo high amounts of EPA and DHA. Once synthesized by microalgae, PUFA are transferred through trophic chain to organisms of higher levels. Thus, aquatic ecosystems play the unique role in the Biosphere as the principal source of EPA and DHA for most omnivorous animals, including inhabitants of terrestrial ecosystems. PUFA are transferred from aquatic to terrestrial ecosystems through riparian predators, drift of carrion and seaweeds, emergence of amphibiotic insects, and water birds. The essential PUFA are transferred through trophic chains with about twice higher efficiency than bulk carbon. Thereby, PUFA are accumulated, rather than diluted in biomass of organisms of higher trophic levels, e.g., in fish. Mankind is faced with a severe deficiency of EPA and DHA in diet. Although additional sources of PUFA supply for humans, such as aquaculture, biotechnology of microorganisms and transgenic terrestrial oil-seed producing plants are developed, natural fish production of aquatic ecosystems will remain one of the main sources of EPA and DHA for humans. Aquatic ecosystems have to be protected from anthropogenic impacts, such as eutrophication, pollution and warming, which reduce PUFA production. В© 2013 Elsevier Inc. All rights reserved.

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Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russia
Siberian Federal University, Svobodny av. 79, Krasnoyarsk 660041, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gladyshev, M.I.; Sushchik, N.N.; Makhutova, O.N.

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