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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zadereev E. S., Lopatina T. S., Ovchinnikov S. D., Oskina N. A., Drobotov, A., V, Tolomeev A. P.
Заглавие : The effect of salinity on the grazing rate and survival of Daphnia magna females adapted to different salinities
Колич.характеристики :14 с
Коллективы : Krasnoyarsk Regional Fund; Krasnoyarsk Krai Government; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [19-44-240010]
Место публикации : Aquat. Ecol.: SPRINGER, 2022. - Article in press. - ISSN 1386-2588, DOI 10.1007/s10452-021-09941-7. - ISSN 1573-5125(eISSN)
Примечания : Cited References:47. - The reported study was funded by the Krasnoyarsk Regional Fund for supporting scientific and technical activities, the Krasnoyarsk Krai Government and the Russian Foundation for Basic Research, project number 19-44-240010. We are grateful to professional English translator Elena Krasova for language corrections.
Предметные рубрики: FEEDING-BEHAVIOR
PHYTOPLANKTON CONTROL
SPECIES COMPOSITION
Аннотация: The cladoceran Daphnia magna inhabits lakes with salinities up to 10 g L-1. We compared the effects of different salinities (up to 9 g L-1) on the survival, specific grazing rate, and size selective feeding of Daphnia females adapted to fresh or saline waters (3-4 g L-1). The freshwater population was more sensitive to high salinity (LC50 = 5.3 g L-1), while the survival of the saline water population also decreased in fresh water. Freshwater population demonstrated a higher grazing rate in fresh water, while the saline water population had a higher grazing rate at the salinity above 3 g L-1. A decrease in the grazing rate of the freshwater population was observed at the salinity above 4-5 g L-1. Populations differed in food selectivity. The saline water population consumed particles of larger sizes than the freshwater population. The average size of phytoplankton particles grazed in fresh water was larger than in saline water. This size selective salinity-dependent grazing may be related to the dependence of the feeding efficiency of cladocerans on the viscosity of water and size of phytoplankton particles. Our results indicate that Daphnia populations adapted to a certain salinity can temporarily lose the ability to control phytoplankton because of salinity fluctuations.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Feniova, I. Yu, Sakharova E. G., Buseva, Zh F., Gladyshev, M., I, Sushchik N. N., Gorelysheva, Z., I, Karpowicz M., Semenchenko V. P.
Заглавие : Efficiency of Transfer of Essential Substances from Phytoplankton to Planktonic Crustaceans in Mesotrophic Conditions
Колич.характеристики :11 с
Коллективы : Polish National Science Centre [UMO-506 2016/21/B/NZ8/00434]; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [18-5400002 Bel_a]; Belarusian Republican Foundation for Fundamental Research [B18R004]; Russian Science FoundationRussian Science Foundation (RSF) [16-14-10323]; [AAAA-A18-118012690096-1]
Место публикации : Inland Water Biol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2021. - Vol. 14, Is. 1. - С. 49-59. - ISSN 1995-0829, DOI 10.1134/S1995082920040033. - ISSN 1995-0837(eISSN)
Примечания : Cited References:39. - The collection of biological material and experimental studies were supported by the Polish National Science Centre (project no. UMO-506 2016/21/B/NZ8/00434). Phytoplankton samples were processed with support from the Russian Foundation for Basic Research (project no. 18-5400002 Bel_a), zooplankton samples were processed and analyzed using the Belarusian Republican Foundation for Fundamental Research (project no. BRFFR no. B18R004), and statistical data analyses were conducted as part of State Task no. AAAA-A18-118012690096-1. The interpretation of the results, review of the literature, and preparation of materials for publication were funded by the Russian Science Foundation (project no. 16-14-10323).
Аннотация: We assessed the efficiency of the transfer of essential substances (carbon, phosphorus, nitrogen, and fatty acids (FA), including polyunsaturated fatty acids (PUFAs)) from phytoplankton to planktonic crustaceans in experimental mesocosms in the presence and absence of fish. The experiments were conducted under mesotrophic conditions in 300 L mesocosms. We have found that transfer efficiencies from producers to consumers are different for different substances. In particular, FA, including PUFAs, are transferred less efficiently than carbon. In contrast, the efficiency of nutrient transfer, especially phosphorus, is higher than that of carbon. This evidences that zooplankton can accumulate nutrients, increasing their quality as a resource for higher trophic levels. Fish significantly reduced the efficiency of carbon transfer from phytoplankton to zooplankton per unit of water volume, but did not affect the transfer of substances per unit of biomass. Thus, the quality of zooplankton as a food resource for higher trophic levels did not decrease in the presence of fish, despite the decline in the efficiency of the transfer of the essential substances per unit of water volume under their influence. Since the efficiency of essential substances transfered from phytoplankton to zooplankton determines the functioning of the entire trophic web, we should seek ways to increase it.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Karpowicz M., Feniova I., Gladyshev M. I., Ejsmont-Karabin J., Gorniak A., Sushchik N. N., Anishchenko O. V., Dzialowski A. R.
Заглавие : Transfer efficiency of carbon, nutrients, and polyunsaturated fatty acids in planktonic food webs under different environmental conditions
Место публикации : Ecology and Evolution: John Wiley and Sons Ltd, 2021. - Article in press. - ISSN 20457758 (ISSN), DOI 10.1002/ece3.7651
Аннотация: The trophic transfer efficiency (TTE) is an important indicator of ecosystem functioning. However, TTE data from freshwater food webs are ambiguous due to differences in time scales and methods. We investigated the transfer of essential substances (carbon, nutrients, and polyunsaturated fatty acids) through plankton communities in 30 Polish lakes with different trophic status in the middle of summer. The results of our study revealed that different essential substances were transferred from phytoplankton to zooplankton with varying efficiencies. The average TTE of C, N, P, and the sum of ?-3 PUFA were 6.55%, 9.82%, 15.82%, and 20.90%, respectively. Our results also show a large mismatch between the elemental and biochemical compositions of zooplankton and their food during the peak of the summer stagnation, which may further promote the accumulation of essential substances. There were also large differences in TTEs between trophic conditions, with the highest efficiencies in oligotrophic lakes and the lowest in dystrophic and eutrophic lakes. Therefore, our study indicates that disturbances like eutrophication and dystrophication similarly decrease the TTE of essential substances between phytoplankton and zooplankton in freshwater food webs. © 2021 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Karpowicz, Maciej, Feniova, Irina, Gladyshev, Michail I., Ejsmont-Karabin, Jolanta, Gorniak, Andrzej, Sushchik, Nadezhda N., Anishchenko, Olesya V., Dzialowski, Andrew R.
Заглавие : Transfer efficiency of carbon, nutrients, and polyunsaturated fatty acids in planktonic food webs under different environmental conditions
Колич.характеристики :14 с
Коллективы : Polish National Science Centre [2016/21/B/NZ8/00434]; Federal Tasks for Institute of Biophysics SB RAS [51.1.1]; Federal Tasks for Siberian Federal University [FSRG-2020-0019]
Место публикации : Ecol. Evol.: WILEY, 2021. - Article in press. - ISSN 2045-7758, DOI 10.1002/ece3.7651
Примечания : Cited References:62. - This research was supported by the Polish National Science Centre (2016/21/B/NZ8/00434). The research was also supported by Federal Tasks for Institute of Biophysics SB RAS No. 51.1.1 and Federal Tasks for Siberian Federal University No. FSRG-2020-0019. The authors are thankful to Joanna Kozowska for her help in the collection of samples.
Предметные рубрики: PHOSPHORUS STOICHIOMETRY
LIGHT-INTENSITY
ZOOPLANKTON
TEMPERATURE
Аннотация: The trophic transfer efficiency (TTE) is an important indicator of ecosystem functioning. However, TTE data from freshwater food webs are ambiguous due to differences in time scales and methods. We investigated the transfer of essential substances (carbon, nutrients, and polyunsaturated fatty acids) through plankton communities in 30 Polish lakes with different trophic status in the middle of summer. The results of our study revealed that different essential substances were transferred from phytoplankton to zooplankton with varying efficiencies. The average TTE of C, N, P, and the sum of omega-3 PUFA were 6.55%, 9.82%, 15.82%, and 20.90%, respectively. Our results also show a large mismatch between the elemental and biochemical compositions of zooplankton and their food during the peak of the summer stagnation, which may further promote the accumulation of essential substances. There were also large differences in TTEs between trophic conditions, with the highest efficiencies in oligotrophic lakes and the lowest in dystrophic and eutrophic lakes. Therefore, our study indicates that disturbances like eutrophication and dystrophication similarly decrease the TTE of essential substances between phytoplankton and zooplankton in freshwater food webs.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondareva, Lydia, Kudryasheva, Nadezhda
Заглавие : Direct and Indirect Detoxification Effects of Humic Substances
Колич.характеристики :13 с
Коллективы : Program of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing (Russian Federation) 2020-2025
Место публикации : Agronomy-Basel: MDPI, 2021. - Vol. 11, Is. 2. - Ст.198. - ISSN 2073-4395(eISSN), DOI 10.3390/agronomy11020198
Примечания : Cited References:79. - This review was prepared with the partial financial support of the Program of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing (Russian Federation) 2020-2025.
Аннотация: The review summarizes studies on the detoxification effects of water-soluble humic substances (HS), which are products of the natural transformation of organic substances in soils and bottom sediments that serve as natural detoxifying agents in water solutions. The detoxifying effects of HS on microorganisms are quite complex: HS neutralize free pollutants (indirect bioeffects) and also stimulate the protective response of organisms (direct bioeffects). Prospects and potential problems of bioluminescent bacteria-based assay to monitor toxicity of solutions in the presence of HS are discussed. The main criterion for the bioassay application is versatility and ease of use. The detoxification efficiency of HS in different pollutant solutions was evaluated, and the detoxification mechanisms are discussed. Particular attention was paid to the direct and complex direct + indirect effects of HS. The review focuses on the protective function of HS in solutions of radionuclides and salts of stable metals, with special consideration of the antioxidant properties of HS.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kratasyuk V. A., Kolosova E. M., Sutormin O. S., Lonshakova-Mukina V. I., Baygin M. M., Rimatskaya N. V., Sukovataya I. E., Shpedt A. A.
Заглавие : Software for matching standard activity enzyme biosensors for soil pollution analysis
Место публикации : Sensors: MDPI AG, 2021. - Vol. 21, Is. 3. - Ст.1017. - С. 1-10. - ISSN 14248220 (ISSN), DOI 10.3390/s21031017
Аннотация: This work is dedicated to developing enzyme biosensor software to solve problems regarding soil pollution analysis. An algorithm and specialised software have been developed which stores, analyses and visualises data using JavaScript programming language. The developed software is based on matching data of 51 non-commercial standard soil samples and their inhibitory effects on three enzyme systems of varying complexity. This approach is able to identify the influence of chemical properties soil samples, without toxic agents, on enzyme biosensors. Such software may find wide use in environmental monitoring. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kratasyuk, Valentina A., Kolosova, Elizaveta M., Sutormin, Oleg S., Lonshakova-Mukina, Viktoriya, I, Baygin, Matvey M., Rimatskaya, Nadezhda, V, Sukovataya, Irina E., Shpedt, Alexander A.
Заглавие : Software for Matching Standard Activity Enzyme Biosensors for Soil Pollution Analysis
Колич.характеристики :10 с
Коллективы : RFBRRussian Foundation for Basic Research (RFBR); Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science [20-44-243001]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Место публикации : Sensors: MDPI, 2021. - Vol. 21, Is. 3. - Ст.1017. - ISSN 1424-8220(eISSN), DOI 10.3390/s21031017
Примечания : Cited References:20. - This research was funded by RFBR, Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science, Grant number 20-44-243001 and the Ministry of Science and Higher Education of the Russian Federation, Grant number FSRZ-2020-0006.
Аннотация: This work is dedicated to developing enzyme biosensor software to solve problems regarding soil pollution analysis. An algorithm and specialised software have been developed which stores, analyses and visualises data using JavaScript programming language. The developed software is based on matching data of 51 non-commercial standard soil samples and their inhibitory effects on three enzyme systems of varying complexity. This approach is able to identify the influence of chemical properties soil samples, without toxic agents, on enzyme biosensors. Such software may find wide use in environmental monitoring.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Feniova I. Y., Sakharova E. G., Buseva Z. F., Gladyshev M. I., Sushchik N. N., Gorelysheva Z. I., Karpowicz M., Semenchenko V. P.
Заглавие : Efficiency of Transfer of Essential Substances from Phytoplankton to Planktonic Crustaceans in Mesotrophic Conditions
Место публикации : Inland Water Biol.: Pleiades journals, 2021. - Vol. 14, Is. 1. - С. 49-59. - ISSN 19950829 (ISSN), DOI 10.1134/S1995082920040033
Аннотация: Abstract: We assessed the efficiency of the transfer of essential substances (carbon, phosphorus, nitrogen, and fatty acids (FA), including polyunsaturated fatty acids (PUFAs)) from phytoplankton to planktonic crustaceans in experimental mesocosms in the presence and absence of fish. The experiments were conducted under mesotrophic conditions in 300 L mesocosms. We have found that transfer efficiencies from producers to consumers are different for different substances. In particular, FA, including PUFAs, are transferred less efficiently than carbon. In contrast, the efficiency of nutrient transfer, especially phosphorus, is higher than that of carbon. This evidences that zooplankton can accumulate nutrients, increasing their quality as a resource for higher trophic levels. Fish significantly reduced the efficiency of carbon transfer from phytoplankton to zooplankton per unit of water volume, but did not affect the transfer of substances per unit of biomass. Thus, the quality of zooplankton as a food resource for higher trophic levels did not decrease in the presence of fish, despite the decline in the efficiency of the transfer of the essential substances per unit of water volume under their influence. Since the efficiency of essential substances transfered from phytoplankton to zooplankton determines the functioning of the entire trophic web, we should seek ways to increase it. © 2021, Pleiades Publishing, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Feniova, I. Yu, Sakharova E. G., Gladyshev, M., I, Sushchik N. N., Gorelysheva, Z., I, Karpowicz M.
Заглавие : EFFECTS OF FISH ON THE TRANSFER EFFICIENCY OF CARBON, PUFA AND NUTRIENTS FROM PHYTOPLANKTON TO ZOOPLANKTON UNDER EUTROPHIC CONDITIONS
Колич.характеристики :15 с
Место публикации : Zool. Zhurnal: MAIK NAUKA-INTERPERIODICA PUBL, 2021. - Vol. 100, Is. 2. - С. 194-208. - ISSN 0044-5134, DOI 10.31857/S0044513421020215
Примечания : Cited References:49
Предметные рубрики: FATTY-ACIDS
FOOD QUALITY
FRESH-WATER
ZEBRA MUSSELS
PHOSPHORUS
Аннотация: The efficiency of the transfer of carbon, polyunsaturated fatty acids (PUFA), nitrogen and phosphorus from phytoplankton to zooplankton determines the functioning of the entire ecosystem. However, this parameter depends on environmental conditions. Fish as a very important factor to regulate planktonic communities are very likely to affect the efficiency of the transfer of basic elements and substances from phytoplankton to zoo plankton. In experimental mesocosms filled with water from an eutrophic lake and containing phyto- and zooplankton, we investigated how fish affect both primary and secondary production and the efficiency of transfer of carbon, nitrogen, phosphorus, PUFA and fatty acids (FA) from phytoplankton to zooplankton. Two treatments (control and fish treatment) were repeated in three replicates. The transfer efficiency of substances from phytoplankton to zooplankton was measured as the ratio of secondary to primary production, expressed in liters and per biomass unit in percent. The efficiency expressed per liter characterizes the productivity of the water body, while the efficiency expressed per biomass unit indicates the effectiveness of aquatic species to transfer biologically valuable substances from one trophic level to another. We found that phytoplankton-zooplankton-fish interactions are determined not only by predator-prey relationships, but also are affected by the quality of both phytoplankton and zooplankton, measured as the contents of phosphorus, nitrogen, PUFA and FA in their biomass. We showed that, in the presence of fish, the transfer efficiency of carbon, phosphorus, nitrogen, eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), FA per biomass unit from phytoplankton to zooplankton was increased two-fold, 12.4-fold, 2,5-fold, 12.4-fold, 7.4-fold, and 10-fold, respectively, relative to control. This provides the sustainability of the functioning of the ecosystem under fish pressure. Such a mechanism prevents zooplankton over-exploitation by fish and enables to prolong the food chain.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Feniova, Irina Yu, Karpowicz, Maciej, Gladyshev, Michail I., Sushchik, Nadezhda N., Petrosyan, Varos G., Sakharova, Ekaterina G., Dzialowski, Andrew R.
Заглавие : Effects of Macrobiota on the Transfer Efficiency of Essential Elements and Fatty Acids From Phytoplankton to Zooplankton Under Eutrophic Conditions
Колич.характеристики :15 с
Коллективы : Polish National Science Centre [2016/21/B/NZ8/00434]; Russian Science FoundationRussian Science Foundation (RSF) [21-14-00123]; Federal Tasks for Institute of Biophysics SB RAS [51.1.1]; Federal Tasks for Siberian Federal University [FSRG-2020-0019]; Polish National Agency for Academic ExchangePolish National Agency for Academic Exchange (NAWA) [PPN/ULM/2020/1/00258/U/DRAFT/00001]
Место публикации : Front. Environ. Sci.: FRONTIERS MEDIA SA, 2021. - Vol. 9. - Ст.739014. - ISSN 2296-665X(eISSN), DOI 10.3389/fenvs.2021.739014
Примечания : Cited References:76. - This experiment was performed with support from the Polish National Science Centre (2016/21/B/NZ8/00434). The statistical analysis and its interpretation was performed with support from the Russian Science Foundation (Grant No. 21-14-00123). Biochemical analyses were performed with support by Federal Tasks for Institute of Biophysics SB RAS No. 51.1.1 and Federal Tasks for Siberian Federal University No. FSRG-2020-0019. The preparation of the manuscript by Feniova I. was supported by the Polish National Agency for Academic Exchange (Agreement No. PPN/ULM/2020/1/00258/U/DRAFT/00001).
Предметные рубрики: FRESH-WATER ZOOPLANKTON
DAPHNIA-LONGISPINA
ZEBRA MUSSELS
Аннотация: The transfer pathways of organic matter and elements from phytoplankton to zooplankton in freshwater ecosystems are important for understanding how aquatic ecosystems function. We conducted a mesocosm experiment to determine how fish and zebra mussels altered the transfer efficiencies of essential substances including carbon (C), polyunsaturated fatty acids (PUFAs), total fatty acids (FAs), phosphorus (P), and nitrogen (N) from phytoplankton to zooplankton. We assessed the transfer efficiencies of the essential substances from phytoplankton to zooplankton as the ratio of their zooplankton production (P) per unit of biomass (B) to that of phytoplankton to exclude grazing or predation effects. We hypothesized that zebra mussels and fish would affect the transfer of materials from phytoplankton to zooplankton by altering the contents of essential elements and FAs in phytoplankton and zooplankton communities and/or due to shifts in the planktonic community structure mediated by grazing and/or predation. Fish increased the transfer efficiencies of eicosapentaenoic acid 20:5 omega-3 (EPA), docosahexaenoic acid 22:6 omega-3 (DHA), and P relative to the control. We speculated that fish weakened the control of zooplankton over algal assemblage by selectively feeding on larger cladocerans such as Daphnia. Therefore, fish can increase the relative proportion of high-quality food for zooplankton, improving food conditions for the available zooplankton. In contrast, zebra mussels reduced the transfer efficiencies of EPA and DHA relative to the control treatment likely due to competition with zooplankton for PUFA-rich food particles. However, zebra mussels did not have any impact on the transfer efficiencies of C, total FAs, N, and P. EPA, DHA, and P were transferred more efficiently than C from phytoplankton to zooplankton, while total FAs, which are commonly used as an energetic source, were transferred as efficiently as C. The enrichment of consumers with the most important substances relative to their basal food sources creates the potential for the successful transport of these substances across aquatic trophic webs./p
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vijayamma, Raji, Maria, Hanna J., Thomas, Sabu, Shishatskaya, Ekaterina I., Kiselev, Evgeniy G., Nemtsev, Ivan V., Sukhanova, Anna A., Volova, Tatiana G.
Заглавие : A study of the properties and efficacy of microparticles based on P(3HB) and P(3HB/3HV) loaded with herbicides
Колич.характеристики :13 с
Коллективы : Project "Agropreparations of the new generation: a strategy of construction and realization" [074-02-2018-328]; Government of the Russian Federation [220]
Место публикации : J. Appl. Polym. Sci.: WILEY, 2021. - Ст.e51756. - Article in press. - ISSN 0021-8995, DOI 10.1002/app.51756. - ISSN 1097-4628(eISSN)
Примечания : Cited References:57. - This work was supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 074-02-2018-328) in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning". Instruments of Krasnoyarsk Regional Center of Research Equipment of Federal Research Center Krasnoyarsk Science Center SB RAS were used.
Предметные рубрики: FENOXAPROP-P-ETHYL
CONTROLLED-RELEASE
BIODEGRADABLE
Аннотация: The wide use of pesticides in agriculture has caused uncontrolled distribution of these chemicals in the environment, calling for the development and investigation of new environmentally friendly formulations, which would reduce human impact on nature. In the present study, the metribuzin (MET), tribenuron-methyl (TBM), and fenoxaprop-P-ethyl (FPE) herbicides were encapsulated in microparticles of degradable microbial polymers - polyhydroxyalkanoates (PHAs) - of two types - poly-3-hydroxybutyrate [P(3HB)] and poly(3-hydroxybutyrate-co-3-hydroxyvalerate [P(3HB/3HV)]. The use of P(3HB) resulted in higher yields of microparticles (63% to 79%) and larger sizes of the particles, whose average diameter was 0.60 +/- 0.06-0.75 +/- 0.11 mu m, while the average diameter of copolymer particles varied between 0.43 +/- 0.12 and 0.55 +/- 0.05 mu m. Encapsulation efficiency was rather determined by the type of herbicide and its solubility, varying from 24.7% to 48.2%. In vitro herbicide release from microparticles to water was affected by herbicide solubility and PHA chemical composition. The readily soluble MET showed the highest release rate, and over 30 days, 64% and 78% of the encapsulated amounts were released from P(3HB) and P(3HB/3HV) microparticles, respectively. High herbicidal activity of microparticles loaded with metribuzin and tribenuron-methyl was demonstrated in the laboratory stands of the Elsholtzia ciliata weed plant.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Feniova, I. Yu, Sakharova E. G., Gladyshev, M., I, Sushchik N. N., Gorelysheva, Z., I, Karpowicz M.
Заглавие : Effect of Fish on the Transfer Efficiency of Carbon, PUFA, and Nutrients from Phytoplankton to Zooplankton under Eutrophic Conditions
Колич.характеристики :14 с
Коллективы : National Science Center of PolandNational Science Centre, Poland [UMO-506 2016/21/B/NZ8/00434]; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [18-54-00002 Bel_a]; Belarusian Republican Foundation for Basic Research (BRFFR) [B18R-004]; Russian Science FoundationRussian Science Foundation (RSF) [16-14-10323]; [AAAA-A18-118012690096-1]
Место публикации : Biol. Bull: PLEIADES PUBLISHING INC, 2021. - Vol. 48, Is. 8. - С. 1284-1297. - ISSN 1062-3590, DOI 10.1134/S1062359021080070. - ISSN 1608-3059(eISSN)
Примечания : Cited References:49. - Experiments and collection of biological material were supported by the National Science Center of Poland (project no. UMO-506 2016/21/B/NZ8/00434). Processing of phytoplankton samples was carried out with the financial support of the Russian Foundation for Basic Research (project no. 18-54-00002 Bel_a); processing of zooplankton samples and their analysis were carried out with the financial support of the Belarusian Republican Foundation for Basic Research (BRFFR no. B18R-004); statistical processing and analysis of data were performed under government contract no. AAAA-A18-118012690096-1; and interpretation of the results, data analysis, and preparation of materials for publication, were done with the financial support of the Russian Science Foundation (project no. 16-14-10323).
Предметные рубрики: FATTY-ACIDS
FOOD QUALITY
FRESH-WATER
ZEBRA MUSSELS
DAPHNIA
Аннотация: The efficiency of the transfer of carbon, polyunsaturated fatty acids (PUFA), nitrogen, and phosphorus from phytoplankton to zooplankton determines the functioning of the entire ecosystem. However, this parameter depends on environmental conditions. Fish as a very important factor regulating planktonic communities very likely affect the efficiency of the transfer of basic elements and substances from phytoplankton to zooplankton. In experimental mesocosms filled with water from a eutrophic lake and containing phyto- and zooplankton, we investigated how fish affect both primary and secondary production and the efficiency of transfer of carbon, nitrogen, phosphorus, PUFA, and fatty acids (FAs) from phytoplankton to zooplankton. Two treatments (control and fish treatment) were repeated in three replicates. The transfer efficiency of substances from phytoplankton to zooplankton was measured as the ratio of secondary production to primary production expressed per L and per unit of biomass. The efficiency expressed per L characterizes the productivity of the water body, while the efficiency expressed per unit of biomass indicates the effectiveness of aquatic species to transfer biologically valuable substances from one trophic level to another. We found that phytoplankton-zooplankton-fish interface is determined not only by predator-prey relationships, but are also affected by the quality of both phytoplankton and zooplankton in terms of contents of phosphorus, nitrogen, PUFA, and FAs in their biomass. We showed that, in the presence of fish, the transfer efficiency of carbon, phosphorus, nitrogen, eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and FAs per unit of biomass from phytoplankton to zooplankton was increased twofold, 12.4-fold, 2.5-fold, 12.4-fold, 7.4-fold, and tenfold, respectively, relative to the control. This facilitates sustainable functioning of the ecosystem under fish pressure. Such a mechanism prevents zooplankton over-exploitation by fish and enlarges the food chain.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Larionova, Marina D., Wu, Lijie, Eremeeva, Elena, V, Natashin, Pavel, V, Gulnov, Dmitry, V, Nemtseva, Elena, V, Liu, Dongsheng, Liu, Zhi-Jie, Vysotski, Eugene S.
Заглавие : Crystal structure of semisynthetic obelin-v
Колич.характеристики :16 с
Коллективы : National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [32011530076]; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [20-04-00085, 20-44-240006, 20-54-53011]
Место публикации : Protein Sci.: WILEY, 2021. - Article in press. - ISSN 0961-8368, DOI 10.1002/pro.4244. - ISSN 1469-896X(eISSN)
Примечания : Cited References:69. - National Natural Science Foundation of China, Grant/Award Number: 32011530076; Russian Foundation for Basic Research, Grant/Award Numbers: 20-04-00085, 20-44-240006, 20-54-53011
Предметные рубрики: CA2+-REGULATED PHOTOPROTEIN OBELIN
PHOTOLUMINESCENCE QUANTUM YIELD
Аннотация: Coelenterazine-v (CTZ-v), a synthetic derivative with an additional benzyl ring, yields a bright bioluminescence of Renilla luciferase and its "yellow" mutant with a significant shift in the emission spectrum toward longer wavelengths, which makes it the substrate of choice for deep tissue imaging. Although Ca2+-regulated photoproteins activated with CTZ-v also display red-shifted light emission, in contrast to Renilla luciferase their bioluminescence activities are very low, which makes photoproteins activated by CTZ-v unusable for calcium imaging. Here, we report the crystal structure of Ca2+-regulated photoprotein obelin with 2-hydroperoxycoelenterazine-v (obelin-v) at 1.80 angstrom resolution. The structures of obelin-v and obelin bound with native CTZ revealed almost no difference; only the minor rearrangement in hydrogen-bond pattern and slightly increased distances between key active site residues and some atoms of 2-hydroperoxycoelenterazine-v were found. The fluorescence quantum yield (phi(FL)) of obelin bound with coelenteramide-v (0.24) turned out to be even higher than that of obelin with native coelenteramide (0.19). Since both obelins are in effect the enzyme-substrate complexes containing the 2-hydroperoxy adduct of CTZ-v or CTZ, we reasonably assume the chemical reaction mechanisms and the yields of the reaction products (phi(R)) to be similar for both obelins. Based on these findings we suggest that low bioluminescence activity of obelin-v is caused by the low efficiency of generating an electronic excited state (phi(S)). In turn, the low phi(S) value as compared to that of native CTZ might be the result of small changes in the substrate microenvironment in the obelin-v active site.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sakharova E. G., Karpowicz M., Gladyshev, M., I, Sushchik N. N., Gorelysheva, Z., I, Feniova, I. Yu
Заглавие : Effects of Dreissena polymorpha on the transfer efficiency of carbon, fatty acids, nitrogen, and phosphorus from phytoplankton to zooplankton
Колич.характеристики :13 с
Место публикации : Zhurnal Obshchei Biol.: MEZHDUNARODNAYA KNIGA, 2021. - Vol. 82, Is. 3. - С. 188-200. - ISSN 0044-4596, DOI 10.31857/S0044459621030052
Примечания : Cited References:72
Предметные рубрики: ZEBRA MUSSELS
FRESH-WATER
MICROCYSTIS-AERUGINOSA
DAPHNIA-LONGISPINA
Аннотация: The efficiency of the transfer of physiologically valuable substances including polyunsaturated fatty acids (PUFA), nitrogen, and phosphorus from phytoplankton to zooplankton is extremely important for the functioning of the whole food web. The bivalve mollusk Dreissena polymorpha has a great impact on planktonic communities. However, its effects on the efficiency of transfer of physiologically valuable substances from phytoplankton to zooplankton have not been assessed so far. The goal of our study was to assess the effect of zebra mussels on the transfer efficiency of carbon, fatty acids including PUFA, nitrogen, and phosphorus from phytoplankton to zooplankton under mesotrophic conditions. In the experimental mesocosms filled with water from a mesotrophic lake containing phyto- and zooplankton, we manipulated the presence/absence of zebra mussels. The experiments showed that zebra mussels decreased the efficiency of the transfer of carbon, PUFA, nitrogen, and phosphorus from phytoplankton to zooplankton, thus reducing the quality of zooplankton as a food resource for higher trophic levels. However, zooplankton revealed the ability to increase the content of physiologically valuable substances in their tissues relative to their contents in the diet. We believe that the mechanism of accumulation of physiologically valuable substances by zooplankton enables to at least partially resist the negative influence of zebra mussels on the quality of zooplankton as a food resource.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tikhomirov, Alexander A.
Заглавие : INTRACANOPY LIGHTING IN PHYTOCENOSES AND PHOTOBIOLOGICAL EFFICIENCY OF RADIATION IN PHOTOCULTURE CONDITIONS
Колич.характеристики :12 с
Коллективы : Biophysics Institute of the Federal Research Centre "Krasnoyarsk Research Facility of the Siberian Branch of the Russian Academy of Sciences" [VI.56.1.4, 0287-2019-0009]
Место публикации : Light Eng.: ZNACK PUBLISHING HOUSE, 2021. - Vol. 29, Is. 2. - С. 4-15. - ISSN 0236-2945, DOI 10.33383/2020-076
Примечания : Cited References:42. - The work is performed as part of state assignments VI.56.1.4 and 0287-2019-0009 "Research of the Effect of Plant Texture on Photosynthesis Efficiency" with the Biophysics Institute of the Federal Research Centre "Krasnoyarsk Research Facility of the Siberian Branch of the Russian Academy of Sciences".
Предметные рубрики: DIFFERENT SPECTRAL COMPOSITION
EMITTING-DIODES
GREEN LIGHT
LETTUCE
Аннотация: The review is devoted to the study of the internal radiation regime in the canopies cultivated under controlled environmental conditions. The expediency of using canopies as an object of research for evaluating the photobiological efficiency of radiation in light culture conditions is justified. The appropriateness of light measurements in multi-tiered canopies is shown, taking into account the role of leaves of different tiers in the formation of an economically useful crop. The main requirements for light devices for their use in measuring artificial radiation in light culture conditions are considered, and a brief analysis of the existing instrument base for performing these studies is given. A number of examples show the complexity and ambiguity of the internal structure of the light field that is forming within canopies in light culture conditions. Conceptual approaches to the choice of spectral and energy characteristics of artificial irradiation for plant light culture are proposed and justified. The necessity of taking into account the light conditions of leaves of different tiers when choosing the spectral and energy characteristics of light sources for the cultivation of multi-tiered canopies is justified. Techniques, methods, and light sources used for additional intracanopy lighting are analysed. The efficiency of using side illumination of plant canopies and conditions for its implementation are considered. The advantages of the volume distribution of canopies on the most common multi-tiered lighting installations are discussed. Based on the presented mate- rial, we consider ways to improve methodological approaches for evaluating the photobiological effectiveness of artificial radiation in light culture conditions for canopies of cultivated plants, taking into account the features of their architectonics and internal radiation regime.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vijayamma R., Maria H. J., Thomas S., Shishatskaya E. I., Kiselev E. G., Nemtsev I. V., Sukhanova A. A., Volova T. G.
Заглавие : A study of the properties and efficacy of microparticles based on P(3HB) and P(3HB/3HV) loaded with herbicides
Место публикации : J. Appl. Polym. Sci.: John Wiley and Sons Inc, 2021. - Article in press. - ISSN 00218995 (ISSN), DOI 10.1002/app.51756
Аннотация: The wide use of pesticides in agriculture has caused uncontrolled distribution of these chemicals in the environment, calling for the development and investigation of new environmentally friendly formulations, which would reduce human impact on nature. In the present study, the metribuzin (MET), tribenuron-methyl (TBM), and fenoxaprop-P-ethyl (FPE) herbicides were encapsulated in microparticles of degradable microbial polymers – polyhydroxyalkanoates (PHAs) – of two types – poly-3-hydroxybutyrate [P(3HB)] and poly(3-hydroxybutyrate-co-3-hydroxyvalerate [P(3HB/3HV)]. The use of P(3HB) resulted in higher yields of microparticles (63% to 79%) and larger sizes of the particles, whose average diameter was 0.60 ± 0.06–0.75 ± 0.11 ?m, while the average diameter of copolymer particles varied between 0.43 ± 0.12 and 0.55 ± 0.05 ?m. Encapsulation efficiency was rather determined by the type of herbicide and its solubility, varying from 24.7% to 48.2%. In vitro herbicide release from microparticles to water was affected by herbicide solubility and PHA chemical composition. The readily soluble MET showed the highest release rate, and over 30 days, 64% and 78% of the encapsulated amounts were released from P(3HB) and P(3HB/3HV) microparticles, respectively. High herbicidal activity of microparticles loaded with metribuzin and tribenuron-methyl was demonstrated in the laboratory stands of the Elsholtzia ciliata weed plant. © 2021 Wiley Periodicals LLC.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pande, Shubhra, Ranjan, Rajeev, Shuvaev, Anton N., Malinovskaya, Natalia A., Ryazanova, Maria, Salmina, Alla B., Kolenchukova, Oksana A., Kratasyuk, Valentina A.
Заглавие : Dietary buckwheat enhances sirtuin1 without calorie restriction
Колич.характеристики :6 с
Коллективы : Russian Foundation for Basic Research (RFBR)Russian Foundation for Basic Research (RFBR) [16-06-00-439]; Russian Ministry of EducationMinistry of Education and Science, Russian Federation [M 2.2.3]
Место публикации : J. Cereal Sci.: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2020. - Vol. 94. - Ст.103004. - ISSN 0733-5210, DOI 10.1016/j.jcs.2020.103004. - ISSN 1095-9963(eISSN)
Примечания : Cited References:22. - This work was supported by the Russian Foundation for Basic Research (RFBR) [Grant No. 16-06-00-439] and by the Russian Ministry of Education, Post-Doctoral Program of Project "5-100" [Grant No. M 2.2.3].
Предметные рубрики: LIFE-SPAN
STRESS
FLOUR
RATS
Аннотация: In the present investigation, the role of dietary intervention in male Wistar rats (n = 8, 3 groups) was studied to observe absolute sirtuin 1 (SIRT1) levels (expressed as ng mg(-1) total protein) in serum, stomach, liver, and kidney. Dietary buckwheat at 30% (w/w) level of incorporation in the standard diet (Buckwheat Enriched Diet, BED) improved SIRT1 with values 0.933 +/- 0.05, 210 +/- 7, 63.26 +/- 4, and 69.89 +/- 3 in serum, stomach, liver, and kidney respectively when compared to the respective control values of 0.536 +/- 0.03, 156 +/- 23.3, 31.07 +/- 2 and 47.11 +/- 4. Moreover, BED though isocaloric to CR diet, led to weight gain (g) by 63.11 +/- 3.8, ca. 10%, and 40% higher than control (56.27 +/- 5.6) and CR (45.05 +/- 4.1) diet groups. A marked rise in Feed Efficiency Ratio (FER) by ca. 37% while a 30% decrease in Feed Conversion Ratio (FCR) was observed for the BED group which supports unexpected weight gain in rats post-dietary intervention. The results justify the superior nutritional profile of buckwheat laden with essential nutrients, essential proteins, and bioactives. In contrast, Calorie Restriction (CR) resulted in a decline of the total protein content in circulation by 19%, while reduction of total protein in stomach, liver, and kidney was estimated to be 95%, 35.2%, and 27% respectively though SIRT1 values were comparatively the highest in all the samples studied. A 30-fold enhancement of SIRT1 in stomach post CR is presumed to counter enhanced stress in gastric tissues. Therefore, mild to moderate expression of SIRT1 may confer beneficial effects such as delayed aging and stress resistance but exceedingly high SIRT1 may evoke increased oxidative stress.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Anishchenko O. V., Tolomeev A. P., Ivanova E. A., Drobotov A. V., Kolmakova A. A., Zuev I. V., Gribovskaya I. V.
Заглавие : Accumulation of elements by submerged (Stuckenia pectinata (L.) Borner) and emergent (Phragmites australis (Cav.) Trin. ex Steud.) macrophytes under different salinity levels
Место публикации : Plant Physiol. Biochem.: Elsevier Masson SAS, 2020. - Vol. 154. - С. 328-340. - ISSN 09819428 (ISSN), DOI 10.1016/j.plaphy.2020.05.019
Аннотация: Accumulation of essential/beneficial and non-essential chemical elements by submerged (Stuckenia pectinata (L.) Borner) and emergent (Phragmites australis (Cav.) Trin. ex Steud.) rooted macrophytes in three water bodies was studied as dependent on water salinity (specific conductivity) and main environmental factors (temperature, pH of water, growing period, element contents in water and sediments). Inductively coupled plasma optical emission spectrometry (ICP - OES) was used for the element detection in the plants, sediments, and water. Multivariate statistics redundancy analysis (RDA) showed that investigated factors together explained 37.8 - 44.7% and 29.9 - 42.6% of the essential/beneficial and non-essential elements variability, respectively, whereas salinity was one of the main factors determining the accumulation of a number of elements (Mg, S, Na, Li, Sr, Sb) by the macrophytes. A decrease in the essential element contents (K, P, N, Cu, Zn) was observed in both species of the macrophytes during the growing season. Environmental factors (salinity, growing period, and pH) were more significant than the element contents in water and the sediments for the essential/beneficial element accumulation by two species of the macrophytes. The element contents in the sediments to a greater extent determined non-essential element accumulation by P. australis than by S. pectinata. The study showed that the efficiency of using P. australis and S. pectinata for remediation of aquatic ecosystems contaminated by heavy metals should not decrease with a salinity increase. © 2020 Elsevier Masson SAS
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pande S., Ranjan R., Shuvaev A. N., Malinovskaya N. A., Ryazanova M., Salmina A. B., Kolenchukova O. A., Kratasyuk V. A.
Заглавие : Dietary buckwheat enhances sirtuin1 without calorie restriction
Место публикации : J. Cereal Sci.: Academic Press, 2020. - Vol. 94. - Ст.103004. - ISSN 07335210 (ISSN), DOI 10.1016/j.jcs.2020.103004
Аннотация: In the present investigation, the role of dietary intervention in male Wistar rats (n = 8, 3 groups) was studied to observe absolute sirtuin 1 (SIRT1) levels (expressed as ng mg?1 total protein) in serum, stomach, liver, and kidney. Dietary buckwheat at 30% (w/w) level of incorporation in the standard diet (Buckwheat Enriched Diet, BED) improved SIRT1 with values 0.933 ± 0.05, 210 ± 7, 63.26 ± 4, and 69.89 ± 3 in serum, stomach, liver, and kidney respectively when compared to the respective control values of 0.536 ± 0.03, 156 ± 23.3, 31.07 ± 2 and 47.11 ± 4. Moreover, BED though isocaloric to CR diet, led to weight gain (g) by 63.11 ± 3.8, ca. 10%, and 40% higher than control (56.27 ± 5.6) and CR (45.05 ± 4.1) diet groups. A marked rise in Feed Efficiency Ratio (FER) by ca. 37% while a 30% decrease in Feed Conversion Ratio (FCR) was observed for the BED group which supports unexpected weight gain in rats post-dietary intervention. The results justify the superior nutritional profile of buckwheat laden with essential nutrients, essential proteins, and bioactives. In contrast, Calorie Restriction (CR) resulted in a decline of the total protein content in circulation by 19%, while reduction of total protein in stomach, liver, and kidney was estimated to be 95%, 35.2%, and 27% respectively though SIRT1 values were comparatively the highest in all the samples studied. A 30-fold enhancement of SIRT1 in stomach post CR is presumed to counter enhanced stress in gastric tissues. Therefore, mild to moderate expression of SIRT1 may confer beneficial effects such as delayed aging and stress resistance but exceedingly high SIRT1 may evoke increased oxidative stress. © 2020 Elsevier Ltd
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Feio, Maria Joao, Filipe, Ana Filipa, Garcia-Raventos, Aina, Ardura, Alba, Calapez, Ana Raquel, Pujante, Ana Maria, Mortagua, Andreia, Murria, Cesc, Diaz-de-Quijano, Daniel, Martins, Filipa M. S., Duarte, Sofia, Bariain, Marta Sainz, Cordeiro, Rita, Rivera, Sinziana F., Vaisanen, Leif O. S., Fonseca, Amelia, Goncalves, Vitor, Garcia-Vazquez, Eva, Rodriguez, David Vieites, Ivanova, Elena A., Costa, Filipe O., Barquin, Jose, Rojo, Veronica, Vierna, Joaquin, Fais, Maria, Suarez, Marcos, Nieminen, Marko, Hammers-Wirtz, Monica, Kolmakova, Olesia, V, Trusova, Maria Y., Beja, Pedro, Gonzalez, Raquel, Planes, Serge, Almeida, Salome F. P.
Заглавие : Advances in the use of molecular tools in ecological and biodiversity assessment of aquatic ecosystems
Колич.характеристики :22 с
Коллективы : MARE strategic program [UID/MAR/04292/2013]; GeoBioTec strategic program [UID/GEO/04035/2019]; Fundo Regional da Ciencia e Tecnologia (FRCT) [M3.1.a/F/017/2011]; FRESHING Project "Next-generation biomonitoring: freshwater bioassessment and species conservation improved with metagenomics" - Portuguese Foundation for Science and Technology (FCT); COMPETE [PTDC/AAG-MAA/2261/2014 -POCI-01-0145-FEDER-356 016824]; FCTPortuguese Foundation for Science and Technology [SFRH/BD/104703/2014, SFRH/BD/113547/2015, SFRH/BPD/109842/2015]; FCT-PhD programme FLUVIO [PD\BD\52510\2014]; Eurostars project [BIOWAT-KIT_E!11892]; Fundacio Aigues de Barcelona
Место публикации : Limnetica: ASOC ESPAN LIMNOL-MISLATA, 2020. - Vol. 39: 19th Congress of the Iberian-Association-of-Limnology (AIL) (JUN 24-29, 2018, Coimbra, PORTUGAL), Is. 1. - С. 419-440. - ISSN 0213-8409, DOI 10.23818/limn.39.27. - ISSN 1989-1806(eISSN)
Примечания : Cited References:92. - We are grateful to all participants of the special session "The use of molecular tools in ecological and biodiversity assessment of aquatic ecosystems" for the productive discussions during the AIL 2018 meeting (XIX Iberian Association of Limnology meeting in Coimbra (Portugal, June 2018). M.J. Feio is supported by MARE strategic program (UID/MAR/04292/2013); SFP Almeida is supported by GeoBioTec strategic program UID/GEO/04035/2019. R. Cordeiro was supported by a Ph.D. Grant (M3.1.a/F/017/2011) from Fundo Regional da Ciencia e Tecnologia (FRCT); A.F. Filipe and A. Garcia-Raventos were supported by FRESHING Project "Next-generation biomonitoring: freshwater bioassessment and species conservation improved with metagenomics" funded by the Portuguese Foundation for Science and Technology (FCT) and COMPETE (PTDC/AAG-MAA/2261/2014 -POCI-01-0145-FEDER-356 016824); F.M.S. Martins was supported by a FCT PhD grant (SFRH/BD/104703/2014); A.R. Calapez was supported by a grant from the FCT-PhD programme FLUVIO (PD\BD\52510\2014); A.M. Pujante acknowledges the BIOWAT-KIT_E!11892 Eurostars project; Maria Fais and Sofia Duarte were supported, respectively, by a PhD (SFRH/BD/113547/2015) and a post-doc fellowship (SFRH/BPD/109842/2015), from FCT; and C. Murria acknowledges the Fundacio Aigues de Barcelona for funding his research.
Предметные рубрики: BARCODE REFERENCE LIBRARY
METABARCODING APPROACH
RAPID ASSESSMENT
Аннотация: Conservation and sustainable management of aquatic ecosystems is a priority in environmental programs worldwide. However, these aims are highly dependent on the efficiency, accuracy and cost of existent methods for the detection of keystone species and monitoring of biological communities. Rapid advances in eDNA, barcoding and metabarcoding promoted by high-throughput sequencing technologies are generating millions of sequences in a fast way, with a promising cost reduction, and overcoming some difficulties of the traditional taxonomic approaches. This paper provides an updated broad perspective of the current developments in this dynamic field presented in the special session (SS) "The use of molecular tools in ecological and biodiversity assessment of aquatic ecosystems" of the XIX Congress of the Iberian Association of Limnology (AIL2018), held in Coimbra, Portugal. Developments presented are mainly focused on the Iberian Peninsula (Portugal and Spain, including Atlantic Macaronesian islands) but include studies in France, Germany, Finland, Russia (Siberia) and South America. The networks within which these researchers are involved are yet even broader, profiting from existing molecular facilities, and traditional taxonomic expertise, which can be viewed as a characteristic of this new research area. It was evident in the SS that the use of molecular tools is widespread, being used to study a diversity of aquatic systems, from rivers' headwaters to estuaries and coastal lagoons, and volcanic, mountain and frozen lakes to hot springs. The organisms targeted are likewise varied and include fish, macroinvertebrates, meiofauna, microalgae such as diatoms and dinoflagellates, other protists, fungi, and bacteria (cyanobacteria and other). Some studies address the whole biodiversity (i.e., all species present independently of the taxonomic group) from environmental samples of water, biofilms and preservative solution from field samples (e.g., ethanol from macroinvertebrate samples). Great advances were acknowledged in the special session, namely in the use of metabarcoding for detecting hidden biodiversity, juvenile stages, low-abundance species, non-indigenous species and toxicity potential, and ultimately for ecological monitoring of diatoms and invertebrates. Yet, several drawbacks were highlighted and need further work, which include: taxonomic gaps in the reference databases (including gaps at species level and on intraspecific variability) or absence of public databases (e.g. for meiofauna), still high sequencing costs, the need of a substantial bioinformatics effort, difficulties in establishing the amount of environmental sample necessary for a good DNA extraction and the need for testing different genetic markers to obtain accurate results.
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