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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : PETUSHKOV V.N., RODIONOVA N.S., BELOBROV P.I.
Заглавие : EFFICIENCY OF THE FUNCTIONING OF THE BIENZYMATIC SYSTEM NADH-FMN OXIDOREDUCTASE LUCIFERASE OF LUMINESCENT BACTERIA
Место публикации : Biochem.-Moscow: PLENUM PUBL CORP, 1985. - Vol. 50, Is. 3. - С. 338-342. - 5. - ISSN 0006-2979
Примечания : Cited References: 13
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : PETUSHKOV V.N., RODIONOVA N.S., BELOBROV P.I.
Заглавие : EFFICIENCY OF THE FUNCTIONING OF THE BIENZYMATIC SYSTEM NADH-FMN OXIDOREDUCTASE LUCIFERASE OF LUMINESCENT BACTERIA
Колич.характеристики :5 с
Место публикации : Biochem.-Moscow: PLENUM PUBL CORP, 1985. - Vol. 50, Is. 3. - P338-342. - ISSN 0006-2979
Примечания : Cited References: 13
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : LOPATIN V.N., SHAPOVALOV K.A.
Заглавие : EVALUATION OF MAXIMAL VALUES FOR THE FACTORS OF LIGHT-SCATTERING EFFICIENCY IN THE WKB APPROXIMATION
Колич.характеристики :4 с
Место публикации : Opt. Spektrosk.: MEZHDUNARODNAYA KNIGA, 1991. - Vol. 71, Is. 3. - P494-497. - ISSN 0030-4034
Примечания : Cited References: 5
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : GORBAN A.N., POPOVA T.G., SADOVSKY M.G.
Заглавие : CORRELATION APPROACH TO COMPARING NUCLEOTIDE-SEQUENCES
Колич.характеристики :11 с
Место публикации : Zhurnal Obshchei Biol.: MEZHDUNARODNAYA KNIGA, 1994. - Vol. 55, Is. 04.05.2013. - P420-430. - ISSN 0044-4596
Примечания : Cited References: 22
Аннотация: A new method of evaluation of similarity between two nucleotide sequences is proposed. It is based on comparing frequency/correlation dictionaries of the sequences under investigation. The dictionary is a set of all strings of various length occurring within the sequences accompanied by their frequencies. The method proposed allows to compare sequences of arbitrary lengths, its advantage is absence of necessity of informal (expert) choice of the best fit. Efficiency of the method is demonstrated by comparison of several human genes and viruses.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kudryasheva N.S., Shalayeva Ye.V., Zadorozhnaya Ye.N., Kratasyuk V.A., Balayan A.E., Stom D.I.
Заглавие : Patterns of inhibition of bacterial bioluminescence in vitro by quinones and phenols - Components of sewage
Место публикации : Biophysics. - 1994. - Vol. 39, Is. 3. - P441-451. - ISSN 00063509 (ISSN)
Аннотация: Quenching of bioluminescence of the bienzyme system bacterial luciferase-NAD В· H:FMN-oxidoreductaseвЂ вЂ NADВ·H is reduced nicotinamide adenine dinucleotide and FMN is flavine manonucleotide. is considered in terms of the patterns of intermolecular transfer of an electron and hydrogen between donor (bioluminescent centre) and acceptor (compound to be analysed) taking into account the structure of the acceptor molecule. The link between the induction period of bioluminescence and the redox potential of the quinone-acceptor has been confirmed. It is shown that the induction period and the time of passage to the bioluminescence maximum in the presence of quinones are determined by the size of the aromatic and aliphatic fragments of the quinone molecule; the intensity of bioluminescence depends on the efficiency of the processes of intermolecular electron transfer (donor-acceptor) and the competition of the compound being analysed with aldehyde for the binding site on luciferase. В© 1994.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : MATVEEV S.V., ILLARIONOV B.A., VYSOTSKI E.S., BONDAR V.S., MARKOVA S.V., ALAKHOV Y.B.
Заглавие : OBELIN MESSENGER-RNA - A NEW TOOL FOR STUDIES OF TRANSLATION IN CELL-FREE SYSTEMS
Колич.характеристики :6 с
Место публикации : Anal. Biochem.: ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS, 1995. - Vol. 231, Is. 1. - С. 34-39. - ISSN 0003-2697, DOI 10.1006/abio.1995.1499
Примечания : Cited References: 17
Предметные рубрики: MESSENGER-RNA
AEQUORIN
PROTEIN
CLONING
CDNA
Аннотация: Obelin mRNA obtained in vitro with the aid of SP6 RNA polymerase was translated in a wheat germ cell-free system, Only the polypeptide with a molecular mass of about 20 kDa was synthesized. The activation of apoobelin with a synthetic coelenterazine revealed a luminescence activity initiated by calcium. The specific activity was 3.6 +/- 0.4 x 10(15) photons per mg of the in vitro synthesized obelin (k = 6.9 s(-1)). The luminescence of the obelin was in a good correlation with the protein concentration calculated by the incorporation of [C-14]Leu. The determination of the amount of de novo synthesized obelin based on measurement of its luminescence is one-thousand times more sensitive than the approach based on the incorporation of labeled amino acid. Thus, obelin mRNA has some advantages for evaluating the efficiency of cell-free translation when compared with standard methods. (C) 1995 Academic Press, Inc.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zadereev E.S., Gubanov V.G.
Заглавие : The role of population density in gamogenesis induction in moina macrocopa (Cladocera: Crustaceae)
Место публикации : Zhurnal Obshchei Biologii. - 1996. - Vol. 57, Is. 3. - С. 366-367. - ISSN 00444596 (ISSN)
Аннотация: Combined influence of population density, temperature and photoperiod on the gamogenesis of individual females of M. macrocopa was investigated. In all experiments the influence of population density was associated with the direct effect of "swarm water". The photoperiod in its broad natural range also influences gamogenesis induction. The increase in temperature above optimum but lower than lethal level sharply decreases the efficiency of gamogenesis. In this case stimulation of gamogenesis is possible by combined effect of poor feeding and high population density. If temperature and photoperiod are optimum for partenogenetic population the change of reproduction mode in lowdensity population of M. macrocopa is determined by poor feeding while in high-density populations density itself is more important. However the increase in food concentration descreases the efficiency of "swarm water" on the gamogenesis induction. According to the hypothesis of nondirect influence of population density an individual decreases its filtarion rate (and starves) under "swarm water" condition.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ushakova S.A., Tikhomirov A.A., Volkova E.K., Alekhina E.B., Zavorueva E.N.
Заглавие : Photosynthesis and respiration in plants grown under red and white light
Место публикации : Russian Journal of Plant Physiology. - 1997. - Vol. 44, Is. 3. - С. 317-321. - ISSN 10214437 (ISSN)
Ключевые слова (''Своб.индексиров.''): cucumis sativus--dark respiration--helianthus annuus--line-structured spectrum--lycopersicon esculentum--photosynthesis--plant resistance--red light--respiration in light
Аннотация: Tomato (Lycopersicon esculentum Mill), sunflower (Helianthus annuus L.), and cucumber (Cucumis sativus L.) plants were grown under red or white light at a radiance of 115 W/m2 under controlled conditions. The red spectra were characterized by either two well-distinguished spectral lines (RL1) or only one (RL2). Apparent photosynthesis (Pa), gross photosynthesis (Pg), dark (Rd), and light (R1) respiration, the content of pigments, and the biochemical composition of plants were estimated. Enhancing the destructive red light influence led to a rise in the Pa/R1 and Pa/Pg ratios and to a decrease in chlorophyll a, nitrate, and reduced nitrogen. RL1 was more inhibitory for plants than RL2. It is concluded that declined energy efficiency of respiration contributes to the destructive effect of RL in sunflower and cucumber plants. The rearrangement of metabolic processes, at which energy efficiency of respiration is maintained, is responsible for adaptation of tomato plants to red light. The obtained results allow one to outline the ways to study the species-specific mechanisms of plant responses to prolonged RL action.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kargatova T.V., Maksimova E.E., Popova L.Yu., Bril'Kov A.V., Pechurkin N.S.
Заглавие : Phenotypic variability of the population of a recombinant luminescent strain of escherichia coli in aqueous microcosms
Место публикации : Mikrobiologiya. - 1997. - Vol. 66, Is. 1. - С. 101-106. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): article--escherichia coli--genetic recombination--genetics--luminescence--microbiology--penicillin resistance--phenotype--plasmid--ampicillin resistance--escherichia coli--luminescent measurements--phenotype--plasmids--recombination, genetic--water microbiology
Аннотация: The behavior of Escherichia coli Z905, carrying a recombinant plasmid pPHL7 with genes determining ampicillin resistance and bacterial luminescence, and the efficiency of expression of cloned genes were studied after introduction of the strain into model aqueous ecosystems with different trophic chain lengths. The E. coli Z905 variants isolated from ecosystems after different periods of time were found to vary in their resistance to ampicillin (from 50 to 0.05 ?g/ml) and in the intensity of bioluminescence. An increase in the concentration of the selective factor (ampicillin) or in the extent of the aqueous microcosm blooming restored the expression of the recombinant plasmid genes in some clones.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kargatova T.V., Maksimova E.E., Popova L.Yu., Bril'kov A.V., Pechurkin N.S.
Заглавие : Phenotypic variability of the population of a recombinant luminescent strain of escherichia coli in aqueous microcosms
Место публикации : Microbiology. - 1997. - Vol. 66, Is. 1. - С. 85-90. - ISSN 00262617 (ISSN)
Аннотация: The behavior of Escherichia coli Z905, carrying a recombinant plasmid pPHL7 with genes determining ampicillin resistance and bacterial luminescence, and the efficiency of expression of cloned genes were studied after introduction of the strain into model aqueous ecosystems with different trophic chain lengths. The E. coli Z905 variants isolated from ecosystems after different periods of time were found to vary in their resistance to ampicillin (from 50 to 0.05 ?g/ml) and in the intensity of bioluminescence. An increase in the concentration of the selective factor (ampicillin) or in the extent of the aqueous microcosm blooming restored the expression of the recombinant plasmid genes in some clones. В© 1997 MAHK Hayka/Interperiodica Publishing.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kargatova T.V., Maksimova E.E., Popova L.Y., Brilkov A.V., Pechurkin N.S.
Заглавие : Phenotypic variability of the population of a recombinant luminescent strain of Escherichia coli in aqueous microcosms
Колич.характеристики :6 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1997. - Vol. 66, Is. 1. - С. 85-90. - ISSN 0026-2617
Примечания : Cited References: 12
Предметные рубрики: GENETICALLY-MODIFIED MICROORGANISMS
Аннотация: The behavior of Escherichia coil Z905, carrying a recombinant plasmid pPHL7 with genes determining ampicillin resistance and bacterial luminescence, and the efficiency of expression of cloned genes were studied after introduction of the strain into model aqueous ecosystems with different trophic chain lengths. The E. coli Z905 variants isolated from ecosystems after different periods of time were found to vary in their resistance to ampicillin (from 50 to 0.05 mu g/ml) and in the intensity of bioluminescence. An increase in the concentration of the selective factor (ampicillin) or in the extent of the aqueous microcosm blooming restored the expression of the recombinant plasmid genes in some clones.
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12.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Sarangova A.B., Somova L.A.
Заглавие : A new enzymatic technique to estimate the efficiency of microbial degradation of pollutants
Колич.характеристики :4 с
Место публикации : LIFE SCIENCES: LIFE SUPPORT SYSTEMS STUDIES-I. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON PRESS LTD, 1997. - Vol. 20: F4.6, F4.8, F4.2 and F4.9 Symposia of COSPAR Scientific Commission F on Life Sciences - Life Support System Studies-I, at the 31st COSPAR Scientific Assembly (JUL 14-SEP 21, 1996, BIRMINGHAM, ENGLAND), Is. 10. - P2049-2052. - ISBN 0273-1177, DOI 10.1016/S0273-1177(97)00940-X. - ISBN 0-08-043307-3
Примечания : Cited References: 4
Аннотация: Dynamics of active sludge microorganism activity in aerotanks under chemostat conditions has been studied. Dependence of microorganism catalase activity has been found to depend on residual substrate concentration in proportion to the biomass of microorganisms. Experimental data and field observations has formed the basis to develop a technique to evaluate in relative units the amount of the substrate consumed by biocenosis of the active sludge in the air tanks of purification facilities. (C) 1997 COSPAR. Published by Elsevier Science Ltd.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sarangova A.B., Somova L.A.
Заглавие : A new enzymatic technique to estimate the efficiency of microbial degradation of pollutants
Место публикации : Advances in Space Research. - 1997. - Vol. 20, Is. 10. - С. 2049-2052. - ISSN 02731177 (ISSN)
Ключевые слова (''Своб.индексиров.''): catalase--hydrogen peroxide--aerobic metabolism--article--bacterium--biomass--bioremediation--enzymology--metabolism--methodology--microbiology--sewage--waste management--water management--aerobiosis--bacteria--biodegradation, environmental--biomass--catalase--hydrogen peroxide--sewage--waste management--water microbiology--water purification
Аннотация: Dynamics of active sludge microorganism activity in aerotanks under chemostat conditions has been studied. Dependence of microorganism catalase activity has been found to depend on residual substrate concentration in proportion to the biomass of microorganisms. Experimental data and field observations has formed the basis to develop a technique to evaluate in relative units the amount of the substrate consumed by biocenosis of the active sludge in the air tanks of purification facilities. В© 1997 COSPAR. Published by Elsevier Science Ltd.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Popova L.I., Maksimova E.E., Kargatova T.V., Bril'kov A.V., Pechurkin N.S.
Заглавие : Introduction and long-term storage of recombinant luminescent Escherichia coli strain Z905 in laboratory water microecosystems
Место публикации : Izvestiia Akademii nauk. Seriia biologicheskaia / Rossiiskaia akademiia nauk. - 1998. - Is. 6. - С. 670-677. - ISSN 10263470 (ISSN)
Ключевые слова (''Своб.индексиров.''): article--bacterial gene--chemoluminescence--escherichia coli--gene expression--genetic recombination--genetics--laboratory--microbiology--chemiluminescent measurements--escherichia coli--gene expression--genes, bacterial--laboratories--recombination, genetic--water microbiology
Аннотация: We studied preservation of recombinant Escherichia coli strain Z905 (AprLux+) in liquid microecosystems (LME) after the introduction. E. coli cells were shown to remain viable and preserve the ability to express the cloned lux genes for a long time (more than a year) in LME. The majority of the clones have reduced efficiency of the expression due to either changed regulation of the lux operon or decreased number of copies of the plasmid. These mechanisms could be realized either independently or simultaneously depending on LME conditions. We have exposed the major factors affecting the metabolic activity of the E. coli strain Z905 (AprLux+) introduced into model ecosystems and the level of expression of the cloned genes.
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15.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Shevyrnogov A..., Trefois P..., Vysotskaya G...
Заглавие : Multi-satellite data merge to combine NOAA AVHRR efficiency with Landsat-6 MSS spatial resolution to study vegetation dynamics
Колич.характеристики :3 с
Место публикации : REMOTE SENSING FOR LAND SURFACE CHARACTERISATION. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON PRESS LTD, 2000. - Vol. 26: A3 1 and A3 2 Symposia of COSPAR Scientific Commission A held at the 32nd COSPAR Scientific Assembly (JUL 12-19, 1998, NAGOYA, JAPAN), Is. 7. - P1131-1133. - ISBN 0273-1177, DOI 10.1016/S0273-1177(99)01130-8
Примечания : Cited References: 2
Аннотация: Spectral range and good replication provided by NOAA AVHRR data make possible to study evolution of vegetation cover and temperature in time. However, the spatial resolution even of 1000 m does not allow accurate location of the changes. The new developed NOAA NDVI image provides better apparent spatial resolution by the following procedure: - resample the NOAA image to 100 m pixel size; - georeference both NOAA and MSS (Landsat-6) to common UTM coordinates; - extract the brightness component from MSS (using the first principal component); - merge NOAA. NDVI and MSS brightness images. The resulting image features the color of NOAA NDVI and topographic details of MSS intensity. This work offers relevant techniques to extract information from satellite imagery to apply to plant dynamics investigation. (C) 2000 COSPAR. Published by Elsevier Science Ltd.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shevyrnogov A., Trefois P., Vysotskaya G.
Заглавие : Multi-satellite data merge to combine NOAA AVHRR efficiency with landsat-6 MSS spatial resolution to study vegetation dynamics
Место публикации : Advances in Space Research. - 2000. - Vol. 26, Is. 7. - С. 1131-1133. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(99)01130-8
Ключевые слова (''Своб.индексиров.''): avhrr--landsat multispectral scanner--noaa satellite--satellite data--spatial resolution--vegetation dynamics
Аннотация: Spectral range and good replication provided by NOAA AVHRR data make possible to study evolution of vegetation cover and temperature in time. However, the spatial resolution even of 1000 m does not allow accurate location of the changes. The new developed NOAA NDVI image provides better apparent spatial resolution by the following procedure: - resample the NOAA image to 100 m pixel size; - georeference both NOAA and MSS (Landsat-6) to common UTM coordinates; - extract the brightness component from MSS (using the first principal component); - merge NOAA NDVI and MSS brightness images. The resulting image features the color of NOAA NDVI and topographic details of MSS intensity. This work offers relevant techniques to extract information from satellite imagery to apply to plant dynamics investigation. (C) 2000 COSPAR. Published by Elsevier Science Ltd.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ganusov V.V., Bril'kov A.V., Pechurkin N.S.
Заглавие : Mathematical modeling of population dynamics of unstable plasmid-containing bacteria during continuous cultivation in a chemostat
Место публикации : Biofizika. - 2000. - Vol. 45, Is. 5. - С. 908-914. - ISSN 00063029 (ISSN)
Ключевые слова (''Своб.индексиров.''): article--bioreactor--escherichia coli--fermentation--genetics--growth, development and aging--photobacterium--plasmid--theoretical model--bioreactors--escherichia coli--fermentation--models, theoretical--photobacterium--plasmids
Аннотация: A structural approach to studying the regularities of the population dynamics of unstable recombinant bacterial strains in a chemostat was elaborated. The approach is based on the mathematical modeling of cell distribution in a population with different numbers of plasmid copies. The effect of decreased selective preference of plasmidless variants of the recombinant strain in the chemostat, which is related to a decrease in the number of plasmid copies in cells upon long-term incubation was analyzed. It is shown that the time of half-elimination of plasmids from the bacterial population in the steady state in the chemostat T1/2 does not depend on the maximum number of plasmid copies in cells N but is determined only by the mean time of generation g and the probability of the loss of one plasmid copy tau. The dependence of the preference of bacterial plasmidless variants on the efficiency of expression of genes cloned into plasmids in chemostat was analyzed using the recombinant strain E. coli Z905, whose plasmids pPHL-7 contain cloned genes for the luminescence system of marine luminescing bacteria Photobacterium leiognathi.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tikhomirov A.A., Lisovskii G.M.
Заглавие : The levels of organization of the photosynthetic apparatus and the control of production processes in phytocenoses under artificial-light culture
Место публикации : Russian Journal of Plant Physiology. - 2001. - Vol. 48, Is. 3. - С. 395-399. - ISSN 10214437 (ISSN) , DOI 10.1023/A:1016682904411
Ключевые слова (''Своб.индексиров.''): artificial-light culture--photosynthetic apparatus--phytocenosis--productivity--embryophyta
Аннотация: The processes limiting the production in higher plant phytocenoses under an artificial-light culture are analyzed in relation to the multilevel organization of the photosynthetic apparatus (PA). The authors consider the feasibility of overcoming these limitations by optimizing the physical parameters of irradiation (the structure of the light spectrum, the rate, and the ratio of radiation fluxes in photosynthetically active radiation (PAR) and infrared (IR) regions) at the molecular, leaf, plant, and cenotic levels of PA organization. To illustrate this approach, the authors used a complex experiment in an artificial ecosystem to evaluate the efficiency of the light control of production processes in multispecies phytocenoses by alleviating or removing the factors that limit plant production at the various levels of PA organization. An artificial-light culture is seen as an instrument for solving several problems of theoretical and applied plant physiology and related disciplines in the future.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tikhomirov A.A., Lisovskii G.M.
Заглавие : The levels of organization of the photosynthetic apparatus and the control of production processes in phytocenoses under artificial-light culture
Колич.характеристики :5 с
Место публикации : Russ. J. Plant Physiol.: MAIK NAUKA/INTERPERIODICA, 2001. - Vol. 48, Is. 3. - P395-399. - ISSN 1021-4437, DOI 10.1023/A:1016682904411
Примечания : Cited References: 21
Ключевые слова (''Своб.индексиров.''): photosynthetic apparatus--phytocenosis--productivity--artificial-light culture
Аннотация: The processes limiting the production in higher plant phytocenoses under an artificial-light culture are analyzed in relation to the multilevel organization of the photosynthetic apparatus (PA). The authors consider the feasibility of overcoming these limitations by optimizing the physical parameters of irradiation (the structure of the light spectrum, the rate, and the ratio of radiation fluxes in photosynthetically active radiation (PAR) and infrared (IR) regions) at the molecular, leaf, plant, and cenotic levels of PA organization. To illustrate this approach, the authors used a complex experiment in an artificial ecosystem to evaluate the efficiency of the light control of production processes in multispecies phytocenoses by alleviating or removing the factors that limit plant production at the various levels of PA organization. An artificial-light culture is seen as an instrument for solving several problems of theoretical and applied plant physiology and related disciplines in the future.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolmakov V.I., Gaevskii N.A., Ivanova E.A., Dubovskaya O.P., Gribovskaya I.V., Kravchuk E.S.
Заглавие : Comparative analysis of ecophysiological characteristics of Stephanodiscus hantzschii Grun. in the periods of its bloom in recreational water bodies
Место публикации : Russian Journal of Ecology. - 2002. - Vol. 33, Is. 2. - С. 97-103. - ISSN 10674136 (ISSN) , DOI 10.1023/A:1014448707663
Ключевые слова (''Своб.индексиров.''): chlorophyll a--efficiency in using photosynthetically active radiation--stephanodiscus hantzschii grun.--algal bloom--comparative study--ecophysiology--photosynthetically active radiation--stephanodiscus hantzschii
Аннотация: In the periods of summer and autumn bloom of the Stephanodiscus hantzschii Crun. in recreational water bodies, studies on the vertical distribution of chlorophyll a, its contents per unit biomass, efficiency in using photosynthetically active radiation (EPhAR), and assimilative activity of microalgae were performed. The results confirmed the existence of two ecophysiological forms of St. hantzschii and provided evidence that both forms are typically autotrophic and can efficiently use low-intensity PhAR for photosynthesis.
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