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Каталог книг и продолжающихся изданий библиотеки Института биофизики СО РАН

Иностранные журналы библиотеки Института биофизики СО РАН

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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kovel E. S., Sachkova A. S., Vnukova N. G., Churilov G. N., Knyazeva E. M., Kudryasheva N. S.
Заглавие : Antioxidant Activity and Toxicity of Fullerenols via Bioluminescence Signaling: Role of Oxygen Substituents
Место публикации : Int J Mol Sci: NLM (Medline), 2019. - Vol. 20, Is. 9. - ISSN 14220067 (ISSN) , DOI 10.3390/ijms20092324
Аннотация: Fullerenols are nanosized water-soluble polyhydroxylated derivatives of fullerenes, a specific allotropic form of carbon, bioactive compounds, and perspective basis for drug development. Our paper analyzes the antioxidant activity and toxicity of a series of fullerenols with different number of oxygen substituents. Two groups of fullerenols were under investigation: (1) C60Oy(OH)x, C60,70Oy(OH)x, where x + y = 24-28 and (2) C60,70Oy(OH)x, Fe0,5C60Oy(OH)x, Gd@C82Oy(OH)x, where x + y = 40-42. Bioluminescent cellular and enzymatic assays (luminous marine bacteria and their enzymatic reactions, respectively) were applied to monitor toxicity in the model fullerenol solutions and bioluminescence was applied as a signaling physiological parameter. The inhibiting concentrations of the fullerenols were determined, revealing the fullerenols' toxic effects. Antioxidant fullerenol' ability was studied in solutions of model oxidizer, 1,4-benzoquinone, and detoxification coefficients of general and oxidative types (DGT and DOxT) were calculated. All fullerenols produced toxic effect at high concentrations (0.01 g L-1), while their antioxidant activity was demonstrated at low and ultralow concentrations (0.001 g L-1). Quantitative toxic and antioxidant characteristics of the fullerenols (effective concentrations, concentration ranges, DGT, and DOxT) were found to depend on the number of oxygen substituents. Lower toxicity and higher antioxidant activity were determined in solutions of fullerenols with fewer oxygen substituents (x + y
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sutormin O. S., Kolosova E. M., Nemtseva A. V., Iskorneva I. V., Lisitsa A. A., Matvienko V. S., Esimbekova A. N., Kratasyuk V. A.
Заглавие : Enzymatic bioassay of soil: Sensitivity comparison of mono-, double- And triple-enzyme systems to soil toxicants
Место публикации : Tsitologiya: Sankt Peterburg, 2018. - Vol. 60, Is. 10. - С. 826-829. - ISSN 00413771 (ISSN) , DOI 10.7868/S0041377118100132
Ключевые слова (''Своб.индексиров.''): bacterial luciferase--bioluminescent analysis--coupled enzyme systems--ecological monitoring--enzymatic toxicity bioassays--lactate dehydrogenase--nadh:fmn-oxidoreductase--soil
Аннотация: In this paper, we have investigated the possibilities of application of enzymatic systems with increasing chain length as a bioassay to evaluate the soil contamination status. The sensitivity of monoenzyme reaction as well as double- and triple-enzyme chains based on NAD(P)H:FMN-oxidoreductase and luciferase of luminous bacteria and lactate dehydrogenase to pesticides and copper ions in water and water extracts from soils were estimated. For this, the toxicological parameter IC 20 reflecting the sensitivity limit of the enzyme system to the to-xicant was used. It was revealed that elongation of the coupled enzyme chain (from mono- to triple-enzyme) increases the sensitivity of the bioassay, in some cases by several orders of magnitude. This pattern can be used as a tool to improve the properties of enzymic bioassays. The effect of extracts from uncontaminated soils of various types on enzymatic systems also differs, which makes possible to design the specialized enzymatic bioassays as well. © 2018 Sankt Peterburg.All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kotlobay, Alexey A., Dubinnyi, Maxim A., Purtov, Konstantin V., Guglya, Elena B., Rodionova, Natalja S., Petushkov, Valentin N., Bolt, Yaroslav V., Kublitski, Vadim S., Kaskova, Zinaida M., Ziganshin, Rustam H., Nelyubina, Yulia V., Dorovatovskii, Pavel V., Eliseev, Igor E., Branchini, Bruce R., Bourenkov, Gleb, Ivanov, Igor A., Oba, Yuichi, Yampolsky, Ilia V., Tsarkova, Aleksandra S.
Заглавие : Bioluminescence chemistry of fireworm Odontosyllis
Колич.характеристики :6 с
Коллективы : Russian Science FoundationRussian Science Foundation (RSF) [18-74-10102, 16-14-00052p]; Air Force Office of Scientific ResearchUnited States Department of DefenseAir Force Office of Scientific Research (AFOSR) [FA9550-18-1-0017]
Место публикации : Proc. Natl. Acad. Sci. U. S. A.: NATL ACAD SCIENCES, 2019. - Vol. 116, Is. 38. - С. 18911-18916. - ISSN 0027-8424, DOI 10.1073/pnas.1902095116
Примечания : Cited References:16. - We thank the late Dr. Shoji Inoue and Dr. Hisae Kakoi (Meijo University) for providing Odontosyllis materials, Sergey Shakhov for photography, and Drs. Mikhail Baranov and Andrey Mikhaylov for discussions. Some experiments were carried out using equipment provided by the Institute of Bioorganic Chemistry of the Russian Academy of Sciences.ore Facility. Some experiments were supported by Planta LLC. Structural and mechanistic studies were supported by Russian Science Foundation Grant 18-74-10102. Isolation, purification, and biochemical studies were supported by Russian Science Foundation Grant 16-14-00052p. B.R.B. acknowledges support from the Air Force Office of Scientific Research (FA9550-18-1-0017).
Предметные рубрики: MECHANISM
DECARBOXYLATION
OXIDATION
Аннотация: Marine polychaetes Odontosyllis undecimdonta, commonly known as fireworms, emit bright blue-green bioluminescence. Until the recent identification of the Odontosyllis luciferase enzyme, little progress had been made toward characterizing the key components of this bioluminescence system. Here we present the biomolecular mechanisms of enzymatic (leading to light emission) and nonenzymatic (dark) oxidation pathways of newly described O. undecimdonta luciferin. Spectral studies, including 1D and 2D NMR spectroscopy, mass spectrometry, and X-ray diffraction, of isolated substances allowed us to characterize the luciferin as an unusual tricyclic sulfur-containing heterocycle. Odontosyllis luciferin does not share structural similarity with any other known luciferins. The structures of the Odontosyllis bioluminescent system's low molecular weight components have enabled us to propose chemical transformation pathways for the enzymatic and nonspecific oxidation of luciferin.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondarenko L. S., Kovel E. S., Kydralieva K. A., Dzhardimalieva G. I., Illes E., Tombacz E., Kicheeva A. G., Kudryasheva N. S.
Заглавие : Effects of modified magnetite nanoparticles on bacterial cells and enzyme reactions
Место публикации : Nanomaterials: MDPI AG, 2020. - Vol. 10, Is. 8. - Ст.1499. - С. 1-20. - ISSN 20794991 (ISSN), DOI 10.3390/nano10081499
Аннотация: Current paper presents biological effects of magnetite nanoparticles (MNPs). Analyzing effects of MNP’ characteristics (zeta-potential and hydrodynamic diameters) on bacteria and their enzyme reactions was the main focus. Photobacterium phosphoreum and bacterial enzymatic reactions were chosen as bioassays. Three types of MNPs were under study: bare Fe3O4, Fe3O4 modified with 3-aminopropyltriethoxysilane (Fe3O4/APTES), and humic acids (Fe3O4/HA). Effects of the MNPs were studied at a low concentration range (< 2 mg/L) and attributed to availability and oxidative activity of Fe3+, high negative surface charge, and low hydrodynamic diameter of Fe3O4/HA, as well as higher Fe3+ content in suspensions of Fe3O4/HA. Low-concentration suspensions of bare Fe3O4 provided inhibitory effects in both bacterial and enzymatic bioassays, whereas the MNPs with modified surface (Fe3O4/APTES and Fe3O4/HA) did not affect the enzymatic activity. Under oxidative stress (i.e., in the solutions of model oxidizer, 1,4-benzoquinone), MNPs did not reveal antioxidant activity, moreover, Fe3O4/HA demonstrated additional inhibitory activity. The study contributes to the deeper understanding of a role of humic substances and silica in biogeochemical cycling of iron. Bioluminescence assays, cellular and enzymatic, can serve as convenient tools to evaluate bioavailability of Fe3+ in natural dispersions of iron-containing nanoparticles, e.g., magnetite, ferrihydrite, etc. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolosova E. M., Sutormin O. S., Esimbekova E. N., Lonshakova-Mukina V. I., Kratasyuk V. A.
Заглавие : Set of Enzymatic Bioassays for Assessment of Soil Contamination
Место публикации : Dokl. Biol. Sci.: NLM (Medline), 2019. - Vol. 489, Is. 1. - С. 165-168. - ISSN 16083105 (ISSN), DOI 10.1134/S0012496619060024
Аннотация: A concept of the comprehensive assessment of soil contamination is proposed. According to it, the conclusion regarding the presence of toxic substances in the analyzed sample is based on the inhibition of enzymatic reactions responsible for various functions of a living organism, such as luminescence, respiration, etc. These functions are taken as test functions in classical bioassays with the use of living objects (luminous bacteria, daphnia, algae, and others). The regularities of the impact of different classes of toxicants on the activity of particular enzymes or coupled oligo-enzyme chains have been established. These enzyme reactions are selected as potential test objects: markers of contamination. Three enzyme systems with the maximal sensitivity to different classes of toxicants have been chosen for the set of enzymatic bioassays: butyrylcholinesterase, NAD(P)H:FMN-oxidoreductase + luciferase, and lactate dehydrogenase + NAD(P)H:FMN-oxidoreductase + luciferase. The possibility to use enzymes instead of living organisms in the bioassay of natural complex systems has been shown.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Puzyr A. P., Burov A. E., Medvedeva S. E., Burova O. G., Bondar V. S.
Заглавие : Two forms of substrate for the bioluminescent reaction in three species of basidiomycetes
Место публикации : Mycol.: Taylor and Francis Ltd., 2019. - Vol. 10, Is. 2. - С. 84-91. - ISSN 21501203 (ISSN) , DOI 10.1080/21501203.2019.1583688
Ключевые слова (''Своб.индексиров.''): cold and hot extracts--culture liquid--enzymatic system--hispidin--luminous fungi--substrate of luminescent reaction
Аннотация: The luminescent response of the enzymatic system of Armillaria borealis on the cold and hot extracts from cell-free culture liquids of Inonotus obliquus, Pholiota sp. and A. borealis was examined. The greatest influence on the light emission produced by the luminescent system of A. borealis was provided by the temperature at which the probes were prepared for assay. Boiling a culture liquid on water bath for a few minutes promoted a multifold increase in the luminescence. The results of luminescence assay suggest that the substance involved in the bioluminescent reaction in higher fungi is presented in culture liquids and mycelia in two forms. In one form, it is ready to interact with the enzymatic system and in the second form, it becomes accessible for the reaction after heat treatment. The pool of thermoactivated substance was found to be much large than the amount of the ready accessible one. We suggest that predecessors of hispidin, which is fungal luciferin precursor, are responsible for this phenomenon. They are not involved in bioluminescence at their original state and are converted into the substrate under the influence of high temperature. © 2019, © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petrova A. S., Lukonina A. A., Dementyev D. V., Bolsunovsky A. Ya. , Popov A. V., Kudryasheva N. S.
Заглавие : Protein-based fluorescent bioassay for low-dose gamma radiation exposures
Место публикации : Anal. Bioanal. Chem.: Springer Verlag, 2018. - ISSN 16182642 (ISSN) , DOI 10.1007/s00216-018-1282-5
Ключевые слова (''Своб.индексиров.''): bioassay--enzymes--fluorescence/luminescence--fluorescent protein--gamma radiation--radiotoxicity--efficiency--enzymes--fluorescence--gamma rays--proteins--proton transfer--fluorescence characteristics--fluorescence intensities--fluorescence spectra--fluorescence/luminescence--fluorescent protein--photochemical process--physiological liquids--radiotoxicity--bioassay
Аннотация: The study suggests an application of a coelenteramide-containing fluorescent protein (CLM-CFP) as a simplest bioassay for gamma radiation exposures. “Discharged obelin,” a product of the bioluminescence reaction of the marine coelenterate Obelia longissima, was used as a representative of the CLM-CFP group. The bioassay is based on a simple enzymatic reaction—photochemical proton transfer in the coelenteramide-apoprotein complex. Components of this reaction differ in fluorescence color, providing, by this, an evaluation of the proton transfer efficiency in the photochemical process. This efficiency depends on the microenvironment of the coelenteramide within the protein complex, and, hence, can evaluate a destructive ability of gamma radiation. The CLM-CFP samples were exposed to gamma radiation (137Cs, 2 mGy/h) for 7 and 16 days at 20 °C and 5 °C, respectively. As a result, two fluorescence characteristics (overall fluorescence intensity and contributions of color components to the fluorescence spectra) were identified as bioassay parameters. Both parameters demonstrated high sensitivity of the CLM-CFP-based bioassay to the low-dose gamma radiation exposure (up to 100 mGy). Higher temperature (20 °C) enhanced the response of CLM-CFP to gamma radiation. This new bioassay can provide fluorescent multicolor assessment of protein destruction in cells and physiological liquids under exposure to low doses of gamma radiation. [Figure not available: see fulltext.]. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zhila, Natalia, Shishatskaya, Ekaterina
Заглавие : Properties of PHA bi-, ter-, and quarter-polymers containing 4-hydroxybutyrate monomer units
Колич.характеристики :8 с
Коллективы : Russian Science Foundation [17-15-01352]
Место публикации : Int. J. Biol. Macromol.: ELSEVIER SCIENCE BV, 2018. - Vol. 111. - С. 1019-1026. - ISSN 0141-8130, DOI 10.1016/j.ijbiomac.2018.01.130. - ISSN 1879-0003(eISSN)
Примечания : Cited References:39. - The research was supported by the Russian Science Foundation (grant No. 17-15-01352).
Предметные рубрики: ALCALIGENES-FAECALIS T1
COMAMONAS-ACIDOVORANS
ENZYMATIC DEGRADATION
Ключевые слова (''Своб.индексиров.''): polyhydroxyalkanoates--4-hydroxybutyrate monomer units--physicochemical--and mechanical properties
Аннотация: The present study investigates physicochemical, mechanical, and biological properties of polyhydroxyalkanoate (PHA) copolymers containing 4-hydroxybutyrate (4HB) synthesized in Cupriavidus eutrophus B10646 culture. In poly(3-hydroxybutyrate/4-hydroxybutyrate) [P(3HB/4HB)]bipolymers, 4HB varied between 10.4 and 75.0 mol%; in poly(3-hydroxybutyrate/3-hydroxyvalerate/4-hydroxybutyrate) terpolymers, 4HB constituted 28.7-55.6 mol%; and in poly(3-hydroxybutyrate/3-hydroxyvalerate/4-hydroxybutyrate/3-hydroxyhexanoate) quaterpolymers, 4HB varied between 9.3 and 13.3 mol%. The degree of crystallinity of P(3HB/4HB) copolymers decreased consistently with an increase in 4HB content, reaching 38%. The incorporation of 3-hydroxyvalerate and 3-hydroxyhexanoate into copolymers enhanced that effect. The effect of 4HB monomer units on temperature properties of copolymers was exhibited as lowering of the melting temperature and crystallization temperature, which improved the processing-related properties of the copolymers. All copolymers containing 4HB showed enhanced elongation at break compared to poly(3-hydroxybutyrate). Polymer films prepared from PHA5 with different chemical composition had similar microstructure and porosity and had no toxic effect on mouse fibroblast NIH 3 T3 cells, proving their high biocompatibility. (C) 2018 Elsevier B.V. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sachkova A. S., Kovel E. S., Churilov G. N., Stom D. I., Kudryasheva N. S.
Заглавие : Biological activity of carbonic nano-structures—comparison via enzymatic bioassay
Место публикации : J. Soils Sed.: Springer Verlag, 2018. - Article in press. - ISSN 14390108 (ISSN) , DOI 10.1007/s11368-018-2134-9
Аннотация: Purpose: The aim of the work is to compare the biological activity of carbonic nano-structures of natural and artificial origination, namely, humic substances (HS) and fullerenols. Materials and methods: The representative of the fullerenol group, С60Оy(OH)x where у + x = 20–22, was chosen. Enzyme-based luminescent bioassay was applied to evaluate toxicity and antioxidant properties of HS and fullerenol (F); chemiluminescent luminol method was used to study a content of reactive oxygen species (ROS) in the solutions. Toxicity of the bioactive compounds was evaluated using effective concentrations ЕС50; detoxification coefficients DOxT were applied to study and compare antioxidant activity of the compounds. Antioxidant activity and ranges of active concentrations of the bioactive compounds were determined in model solutions of organic and inorganic oxidizers—1,4-benzoquinone and potassium ferricianide. Results and discussion: Values of ЕС50 revealed higher toxicity of HS than F (0.005 and 0.108 g L?1, respectively); detoxifying concentrations of F were found to be lower. Antioxidant ability of HS was demonstrated to be time-dependent; the 50-min preliminary incubation in oxidizer solutions was suggested as optimal for the detoxification procedure. On the contrary, F’ antioxidant effect demonstrated independency on time. Antioxidant effect of HS did not depend on amphiphilic characteristics of the media (values of DOxT were 1.3 in the solutions of organic and inorganic oxidizers), while this of F was found to depend: it was maximal (DOxT = 2.0) in solutions of organic oxidizer, 1,4-benzoquinone. Conclusions: Both HS and F demonstrated toxicity and low-concentration antioxidant ability; however, quantitative characteristics of their effects were different. The differences were explained with HS polyfunctionality, higher ability to decrease ROS content, non-rigidity, and diffusion restrictions in their solutions. Antioxidant effect of the bioactive compounds was presumably attributed to catalytic redox activity of their ?-fragments. The paper demonstrates a high potential of luminescent enzymatic bioassay to study biological activity of nano-structures of natural and artificial origination. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Lukyanenko, Kirin A., Denisov, Ivan A., Sorokin, Vladimir V., Yakimov, Anton S., Esimbekova, Elena N., Belobrov, Peter, I
Заглавие : Handheld Enzymatic Luminescent Biosensor for Rapid Detection of Heavy Metals in Water Samples
Колич.характеристики :10 с
Коллективы : Russian Foundation for Basic Research, Government of Krasnoyarsk Territory [18-44-242003]
Место публикации : Chemosensors: MDPI, 2019. - Vol. 7, Is. 1. - Ст.16. - ISSN 2227-9040, DOI 10.3390/chemosensors7010016
Примечания : Cited References:39. - This research was funded by Russian Foundation for Basic Research, Government of Krasnoyarsk Territory, Krasnoyarsk Regional Fund of Science, to the research project #18-44-242003: "Designing an enzyme reagent for bioluminescent analysis: mechanisms for increasing sensitivity and accuracy".
Предметные рубрики: ON-A-CHIP
SILICON PHOTOMULTIPLIER
OPTICAL BIOSENSORS
CELL
Аннотация: Enzymatic luminescent systems are a promising tool for rapid detection of heavy metals ions for water quality assessment. Nevertheless, their widespread use is limited by the lack of test procedure automation and available sensitive handheld luminometers. Herein we describe integration of disposable microfluidic chips for bioluminescent enzyme-inhibition based assay with a handheld luminometer, which detection system is based on a thermally stabilized silicon photomultiplier (SiPM). Microfluidic chips were made of poly(methyl methacrylate) by micro-milling method and sealed using a solvent bonding technique. The composition of the bioluminescent system in microfluidic chip was optimized to achieve higher luminescence intensity and storage time. Results indicate that developed device provided comparable sensitivity with bench-scale PMT-based commercial luminometers. Limit of detection for copper (II) sulfate reached 2.5 mg/L for developed biosensor. Hereby we proved the concept of handheld enzymatic optical biosensors with disposable chips for bioassay. The proposed biosensor can be used as an early warning field-deployable system for rapid detection of heavy metals salts and other toxic chemicals, which affect bioluminescent signal of enzymatic reaction.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Eremeeva, Elena V., Vysotski, Eugene S.
Заглавие : Exploring Bioluminescence Function of the Ca2+-regulated Photoproteins with Site-directed Mutagenesis
Колич.характеристики :16 с
Коллективы : Russian Foundation for Basic Research [17-04-00764]; Russian Academy of Sciences [0356-2017-0017]
Место публикации : Photochem. Photobiol.: WILEY, 2019. - Vol. 95, Is. 1. - С. 8-23. - ISSN 0031-8655, DOI 10.1111/php.12945. - ISSN 1751-1097(eISSN)
Примечания : Cited References:88. - This work was supported by grant 17-04-00764 of Russian Foundation for Basic Research and the state budgetallocated to the fundamental research at the Russian Academy of Sciences (project 0356-2017-0017).
Предметные рубрики: CALCIUM-BINDING PHOTOPROTEIN
GREEN-FLUORESCENT PROTEIN
JELLYFISH
Ключевые слова (''Своб.индексиров.''): bioluminescence--coelenterazine--aequorin--obelin--clytin--mitrocomin--ef-hand protein
Аннотация: Site-directed mutagenesis is a powerful tool to investigate the structure-function relationship of proteins and a function of certain amino acid residues in catalytic conversion of substrates during enzymatic reactions. Hence, it is not surprising that this approach was repeatedly applied to elucidate the role of certain amino acid residues in various aspects of photoprotein bioluminescence, mostly for aequorin and obelin, and to design mutant photoproteins with altered properties (modified calcium affinity, faster or slower bioluminescence kinetics, different emission color) which would either allow the development of novel bioluminescent assays or improvement of characteristics of the already existing ones. This information, however, is scattered over different articles. In this review, we systematize the findings that were made using site-directed mutagenesis studies regarding the impact of various amino acid residues on bioluminescence of hydromedusan Ca2+-regulated photoproteins. All key residues that have been identified are pinpointed, and their influence on different aspects of photoprotein functioning such as active photoprotein complex formation, bioluminescence reaction, calcium response and light emitter formation is discussed.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondarenko, Lyubov S., Kovel, Ekaterina S., Kydralieva, Kamila A., Dzhardimalieva, Gulzhian, I, Illes, Erzsebet, Tombacz, Etelka, Kicheeva, Arina G., Kudryasheva, Nadezhda S.
Заглавие : Effects of Modified Magnetite Nanoparticles on Bacterial Cells and Enzyme Reactions
Колич.характеристики :20 с
Коллективы : Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [19-315-50048, 19-33-90149, 18-29-19003]
Место публикации : Nanomaterials: MDPI, 2020. - Vol. 10, Is. 8. - Ст.1499. - ISSN 2079-4991(eISSN), DOI 10.3390/nano10081499
Примечания : Cited References:83. - This research was funded by the Russian Foundation for Basic Research (#19-315-50048, #19-33-90149, and #18-29-19003).
Предметные рубрики: NATURAL ORGANIC-MATTER
HUMIC-ACID
DETOXIFICATION PROCESSES
Аннотация: Current paper presents biological effects of magnetite nanoparticles (MNPs). Relations of MNP' characteristics (zeta-potential and hydrodynamic diameters) with effects on bacteria and their enzymatic reactions were the main focus.Photobacterium phosphoreumand bacterial enzymatic reactions were chosen as bioassays. Three types of MNPs were under study: bare Fe3O4, Fe(3)O(4)modified with 3-aminopropyltriethoxysilane (Fe3O4/APTES), and humic acids (Fe3O4/HA). Effects of the MNPs were studied at a low concentration range ( 2 mg/L) and attributed to availability and oxidative activity of Fe3+, high negative surface charge, and low hydrodynamic diameter of Fe3O4/HA, as well as higher Fe(3+)content in suspensions of Fe3O4/HA. Low-concentration suspensions of bare Fe(3)O(4)provided inhibitory effects in both bacterial and enzymatic bioassays, whereas the MNPs with modified surface (Fe3O4/APTES and Fe3O4/HA) did not affect the enzymatic activity. Under oxidative stress (i.e., in the solutions of model oxidizer, 1,4-benzoquinone), MNPs did not reveal antioxidant activity, moreover, Fe3O4/HA demonstrated additional inhibitory activity. The study contributes to the deeper understanding of a role of humic substances and silica in biogeochemical cycling of iron. Bioluminescence assays, cellular and enzymatic, can serve as convenient tools to evaluate bioavailability of Fe(3+)in natural dispersions of iron-containing nanoparticles, e.g., magnetite, ferrihydrite, etc.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Esimbekova, Elena N., Kalyabina, Valeriya P., Kopylova, Kseniya, V, Torgashina, Irina G., Kratasyuk, Valentina A.
Заглавие : Design of bioluminescent biosensors for assessing contamination of complex matrices
Колич.характеристики :9 с
Коллективы : Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR); Government of Krasnoyarsk Territory; Krasnoyarsk Regional Fund of Science [20-44-242001]; Ministry of Science and Higher Education of Russian Federation [FSRZ-2020-0006]
Место публикации : Talanta: ELSEVIER, 2021. - Vol. 233. - Ст.122509. - ISSN 0039-9140, DOI 10.1016/j.talanta.2021.122509. - ISSN 1873-3573(eISSN)
Примечания : Cited References:87. - The reported study was funded by Russian Foundation for Basic Research, Government of Krasnoyarsk Territory, Krasnoyarsk Regional Fund of Science, to the research project No. 20-44-242001 and Ministry of Science and Higher Education of Russian Federation No. FSRZ-2020-0006.
Предметные рубрики: SAMPLE PREPARATION
PESTICIDES
FOOD
BIOMOLECULES
SENSITIVITY
Аннотация: The presence of potentially toxic xenobiotics in complex matrices has become rather the rule than the exception. Therefore, there is a need for highly sensitive inexpensive techniques for analyzing environmental and food matrices for toxicants. Enzymes are selectively sensitive to various toxic compounds, and, thus, they can be used as the basis for detection of contaminants in complex matrices. There are, however, a number of difficulties associated with the analysis of complex matrices using enzyme assays, including the necessity to take into account properties and effects of the natural components of the test media for accurate interpretation of results. The present study describes the six-stage procedure for designing new enzyme sensors intended for assessing the quality of complex matrices. This procedure should be followed both to achieve the highest possible sensitivity of the biosensor to potentially toxic substances and to minimize the effect of the uncontaminated components of complex mixtures on the activity of the biosensor. The proposed strategy has been tested in designing a bioluminescent biosensor for integrated rapid assessment of the safety of fruits and vegetables. The biosensor is based on the coupled enzyme system NAD(P)H:FMN-oxidoreductase and luciferase as the biorecognition element. The study describes methods and techniques for attaining the desired result in each stage. The proposed six-stage procedure for designing bioluminescent enzyme biosensors can be used to design the enzymatic biosensors based on other enzymes.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Esimbekova E. N., Torgashina I. G., Kalyabina V. P., Kratasyuk V. A.
Заглавие : Enzymatic Biotesting: Scientific Basis and Application
Колич.характеристики :15 с
Коллективы : Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [19-14-50238\19]
Место публикации : Contemp. Probl. Ecol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2021. - Vol. 14, Is. 3. - С. 290-304. - ISSN 1995-4255, DOI 10.1134/S1995425521030069. - ISSN 1995-4263(eISSN)
Примечания : Cited References:128. - This study was carried out with financial support from the Russian Foundation for Basic Research, project no. 19-14-50238\19.
Предметные рубрики: ORGANOPHOSPHORUS PESTICIDES
CHRONIC EXPOSURE
BIOSENSOR
Аннотация: The paper provides a review of the current state of research in the field of biotesting, and the problems of environmental studies and ways to solve them are discussed. The basic principles and examples of using enzymes for detecting toxicants in various environmental samples are considered. Based on an analysis of numerous published data, the advantages and limitations, as well as the prospects for using enzymes for performing biotesting tasks, are assessed. A separate section of the review is devoted to bioluminescent enzymatic bioassays developed by the authors and successfully used for environmental monitoring of water, soil, and air. The necessity of developing a battery of enzymatic bioassays is substantiated. It allows one to have the most complete and accurate information about the degree of pollution of environmental objects.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolosova E. M., Sutormin O. S., Stepanova L. V., Shpedt A. A., Rimatskaya N. V., Sukovataya I. E., Kratasyuk V. A.
Заглавие : Bioluminescent enzyme inhibition-based assay for the prediction of toxicity of pollutants in urban soils
Место публикации : Environ. Technol. Innov.: Elsevier B.V., 2021. - Vol. 24. - Ст.101842. - ISSN 23521864 (ISSN), DOI 10.1016/j.eti.2021.101842
Аннотация: There is a need for rapid simple and informative environmental assessment methods. The present investigation is aimed at assessing the possibility of using the combined enzyme system of luminescent bacteria: NAD(P)H:FMN-oxidoreductase + luciferase (Red + Luc) for predicting the potential toxicity of industrial urbostratozems sampled in the city of Krasnoyarsk. Three groups of urbostratozems polluted with fluorine, arsenic and lead, were tested by the methods of chemical analysis and enzymatic bioassay. Only the assessment of the arsenic-contaminated soil samples showed the dependence between the reduced activity of the enzyme system and the arsenic concentration variations. The results reveal that the sensitivity of the Red + Luc enzyme system to the soil pollutants depends on the properties of the studied soil samples. Moreover, the solubility of lead in the soil samples affects the accuracy of the enzymatic bioassays for soil toxicity testing. The results of the enzymatic bioassay of the fluoride-contaminated soil samples are ambiguous. The obtained data show the relevance of the sample preparation during integral bioassays. In addition, soil properties should be taken into account as well. The current study emphasizes the importance of conducting chemical and biological testing as a combined set to obtain comprehensive information about the anthropogenic load. © 2021 Elsevier B.V.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolesnik, Olga V., Rozhko, Tatiana V., Lapina, Maria A., Solovyev, Vladislav S., Sachkova, Anna S., Kudryasheva, Nadezhda S.
Заглавие : Development of Cellular and Enzymatic Bioluminescent Assay Systems to Study Low-Dose Effects of Thorium
Колич.характеристики :13 с
Место публикации : Bioengineering-Basel: MDPI, 2021. - Vol. 8, Is. 12. - Ст.194. - ISSN 2306-5354(eISSN), DOI 10.3390/bioengineering8120194
Примечания : Cited References:77
Аннотация: Thorium is one of the most widespread radioactive elements in natural ecosystems, along with uranium, it is the most important source of nuclear energy. However, the effects of thorium on living organisms have not been thoroughly studied. Marine luminescent bacteria and their enzymes are optimal bioassays for studying low-dose thorium exposures. Luminescent bioassays provide a quantitative measure of toxicity and are characterized by high rates, sensitivity, and simplicity. It is known that the metabolic activity of bacteria is associated with the production of reactive oxygen species (ROS). We studied the effects of thorium-232 (10(-11)-10(-3) M) on Photobacterium phosphoreum and bacterial enzymatic reactions; kinetics of bacterial bioluminescence and ROS content were investigated in both systems. Bioluminescence activation was revealed under low-dose exposures (0.1 Gy) and discussed in terms of "radiation hormesis". The activation was accompanied by an intensification of the oxidation of a low-molecular reducer, NADH, during the enzymatic processes. Negative correlations were found between the intensity of bioluminescence and the content of ROS in bacteria and enzyme systems; an active role of ROS in the low-dose activation by thorium was discussed. The results contribute to radioecological potential of bioluminescence techniques adapted to study low-intensity radioactive exposures.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolosova, Elizaveta M., Sutormin, Oleg S., Stepanova L. V., Shpedt, Aleksandr A., Rimatskaya N. V., Sukovataya, Irina E., Kratasyuk, Valentina A.
Заглавие : Bioluminescent enzyme inhibition-based assay for the prediction of toxicity of pollutants in urban soils
Колич.характеристики :9 с
Коллективы : Russian Foundation for Basic Research, the Government of the Krasnoyarsk Region, Russia; Krasnoyarsk Regional Foundation for Supporting Scientific and Technological Activities, Russia [18-47-240005]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Место публикации : Environ. Technol. Innov.: ELSEVIER, 2021. - Vol. 24. - Ст.101842. - ISSN 2352-1864, DOI 10.1016/j.eti.2021.101842
Примечания : Cited References:46. - This work was supported by the Russian Foundation for Basic Research, the Government of the Krasnoyarsk Region, Russia, and Krasnoyarsk Regional Foundation for Supporting Scientific and Technological Activities, Russia [grant number 18-47-240005] in the field of statistical analysis and interpretation of the data; and the work related to the sample collection was supported by the Ministry of Science and Higher Education of the Russian Federation [grant number FSRZ-2020-0006].
Предметные рубрики: FLUORIDE
BIOASSAYS
POLLUTION
METALS
WATER
Аннотация: There is a need for rapid simple and informative environmental assessment methods. The present investigation is aimed at assessing the possibility of using the combined enzyme system of luminescent bacteria: NAD(P)H:FMN-oxidoreductase + luciferase (Red + Luc) for predicting the potential toxicity of industrial urbostratozems sampled in the city of Krasnoyarsk. Three groups of urbostratozems polluted with fluorine, arsenic and lead, were tested by the methods of chemical analysis and enzymatic bioassay. Only the assessment of the arsenic-contaminated soil samples showed the dependence between the reduced activity of the enzyme system and the arsenic concentration variations. The results reveal that the sensitivity of the Red + Luc enzyme system to the soil pollutants depends on the properties of the studied soil samples. Moreover, the solubility of lead in the soil samples affects the accuracy of the enzymatic bioassays for soil toxicity testing. The results of the enzymatic bioassay of the fluoride-contaminated soil samples are ambiguous. The obtained data show the relevance of the sample preparation during integral bioassays. In addition, soil properties should be taken into account as well. The current study emphasizes the importance of conducting chemical and biological testing as a combined set to obtain comprehensive information about the anthropogenic load. (C) 2021 Elsevier B.V. All rights reserved.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kovel, Ekaterina S., Kicheeva, Arina G., Vnukova, Natalia G., Churilov, Grigory N., Stepin, Evsei A., Kudryasheva, Nadezhda S.
Заглавие : Toxicity and Antioxidant Activity of Fullerenol C-60,C-70 with Low Number of Oxygen Substituents
Колич.характеристики :17 с
Коллективы : RFBRRussian Foundation for Basic Research (RFBR) [N18-29-19003]; RFBR, Krasnoyarsk Territory; Krasnoyarsk Regional Fund of Science [N20-44-243001]; Program of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Fundamental Study 2020-2025 (Russian Federation)
Место публикации : Int. J. Mol. Sci.: MDPI, 2021. - Vol. 22, Is. 12. - Ст.6382. - ISSN 1422-0067(eISSN), DOI 10.3390/ijms22126382
Примечания : Cited References:93. - This research was funded by RFBR, N18-29-19003; RFBR, Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science, N20-44-243001; and partly supported by the Program of the Federal Service for Surveillance on Consumer Rights Protection and HumanWellbeing, Fundamental Study 2020-2025 (Russian Federation).
Предметные рубрики: HUMIC SUBSTANCES
DETOXIFICATION PROCESSES
BIOLOGICAL-ACTIVITY
Аннотация: Fullerene is a nanosized carbon structure with potential drug delivery applications. We studied the bioeffects of a water-soluble fullerene derivative, fullerenol, with 10-12 oxygen groups (F10-12); its structure was characterized by IR and XPS spectroscopy. A bioluminescent enzyme system was used to study toxic and antioxidant effects of F10-12 at the enzymatic level. Antioxidant characteristics of F10-12 were revealed in model solutions of organic and inorganic oxidizers. Low-concentration activation of bioluminescence was validated statistically in oxidizer solutions. Toxic and antioxidant characteristics of F10-12 were compared to those of homologous fullerenols with a higher number of oxygen groups:F24-28 and F40-42. No simple dependency was found between the toxic/antioxidant characteristics and the number of oxygen groups on the fullerene's carbon cage. Lower toxicity and higher antioxidant activity of F24-28 were identified and presumptively attributed to its higher solubility. An active role of reactive oxygen species (ROS) in the bioeffects of F10-12 was demonstrated. Correlations between toxic/antioxidant characteristics of F10-12 and ROS content were evaluated. Toxic and antioxidant effects were related to the decrease in ROS content in the enzyme solutions. Our results reveal a complexity of ROS effects in the enzymatic assay system.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Rozhko, Tatiana V., Nemtseva, Elena V., Gardt, Maria V., Raikov, Alexander V., Lisitsa, Albert E., Badun, Gennadii A., Kudryasheva, Nadezhda S.
Заглавие : Enzymatic Responses to Low-Intensity Radiation of Tritium
Колич.характеристики :15 с
Коллективы : RFBR-Krasnoyarsk Regional Foundation [N 18-44-240004, 18-44-242002]
Место публикации : Int. J. Mol. Sci.: MDPI, 2020. - Vol. 21, Is. 22. - Ст.8464. - ISSN 1422-0067(eISSN), DOI 10.3390/ijms21228464
Примечания : Cited References:59. - This work was supported by RFBR-Krasnoyarsk Regional Foundation N 18-44-240004, 18-44-242002.
Предметные рубрики: LUMINOUS MARINE-BACTERIA
IONIZING-RADIATION
DISCHARGED-OBELIN
Аннотация: The present study considers a possible role of enzymatic reactions in the adaptive response of cells to the beta-emitting radionuclide tritium under conditions of low-dose exposures. Effects of tritiated water (HTO) on the reactions of bacterial luciferase and NAD(P)H:FMN-oxidoreductase, as well as a coupled system of these two reactions, were studied at radioactivity concentrations
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Rozhko T. V., Nemtseva E. V., Gardt M. V., Raikov A. V., Lisitsa A. E., Badun G. A., Kudryasheva N. S.
Заглавие : Enzymatic responses to low-intensity radiation of tritium
Место публикации : Int. J. Mol. Sci.: MDPI AG, 2020. - Vol. 21, Is. 22. - Ст.8464. - С. 1-15. - ISSN 16616596 (ISSN), DOI 10.3390/ijms21228464
Аннотация: The present study considers a possible role of enzymatic reactions in the adaptive response of cells to the beta-emitting radionuclide tritium under conditions of low-dose exposures. Effects of tritiated water (HTO) on the reactions of bacterial luciferase and NAD(P)H:FMN-oxidoreductase, as well as a coupled system of these two reactions, were studied at radioactivity concentrations ? 200 MBq/L. Additionally, one of the simplest enzymatic reactions, photobiochemical proton transfer in Coelenteramide-containing Fluorescent Protein (CLM-FP), was also investigated. We found that HTO increased the activity of NAD(P)H:FMN-oxidoreductase at the initial stage of its reaction (by up to 230%); however, a rise of luciferase activity was moderate (20%). The CLM-FP samples did not show any increase in the rate of the photobiochemical proton transfer under the exposure to HTO. The responses of the enzyme systems were compared to the ‘hormetic’ response of luminous marine bacterial cells studied earlier. We conclude that (1) the oxidoreductase reaction contributes significantly to the activation of the coupled enzyme system and bacterial cells by tritium, and (2) an increase in the organization level of biological systems promotes the hormesis phenomenon. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
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