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Вид документа : Статья из сборника (выпуск продолж. издания)
Шифр издания :
Автор(ы) : Tikhomirov A.A., Gitelson J.I., Ushakova S.A., Kovaleva N.P., Tikhomirova N.A., Gribovskaya I.V.
Заглавие : The possible way of introducing mineral elements of liquid human wastes into the material cycle in biological life support systems
Место публикации : International Astronautical Federation - 55th International Astronautical Congress 2004. - 2004. - Vol. 3: International Astronautical Federation - 55th International Astronautical Congress 2004 (4 October 2004 through 8 October 2004, Vancouver) Conference code: 69653. - С. 1442-1448
Ключевые слова (''Своб.индексиров.''): biomass--body fluids--hydrogen peroxide--life support systems (spacecraft)--solid wastes--biological life support systems--intrasystem material cycle--liquid human wastes--plant biomass--waste management
Аннотация: Along with the atmosphere, water and food regeneration processes in biological life support systems it is important to provide units and links responsible for utilization of unused plant biomass, human wastes and returning, if possible, the most of wastes into the intrasystem material cycle. The experience on construction of biological life support systems (BLSS) gained by the Institute of Biophysics SB RAS (Krasnoyarsk, Russia) allows us to suggest constructing an integrated biological-physical-chemical life support system with the biological unit predominating. It is possibly to partially mineralize urine and solid wastes by "wet incineration" by hydrogen peroxide in electric field. We suggest decomposing urea by a urease-enzymatic method using soybean or canavalia flour containing sufficient amount of urease. Consumption of 1.5 g of flour for decomposition of urea in daily urine and the possibility of producing flour from soybeans and canavalia grown inside the system make this method of urea decomposition rather prospective. Further ammonia distillation using the nitrification unit and evaporation of solution would make possible to return nitrogen and water back into the intrasystem cycle. Probably, in long-duration space expeditions the utilization of urine would be confined only by extraction of nitrogen and water from urine with further removal of dry residue to the stock, as the problem of returning sodium chloride into the intrasystem cycling has not been solved yet. As all biogenic elements contained in urine (except nitrogen) get lost at that, the solution of the problem with introducing NaCl and mineral elements into the cycle with the help of halophyte plants Salicornia europaea are of sufficient interest. This work presents the experimental results of growing Salicornia europaea on model solutions containing biogenic elements in the amounts equivalent to their content in urine and on urine, which undergone physically-chemically treatment by peroxide and ammonia distillation after urease-enzymatic decomposition. Taking into consideration that the mineral elements content in urine can vary, 2 variants of model solutions were used. In the first variant the content of P was 8-fold, S - 7-fold, K - 8-fold higher than in Knop's solution; the content of Ca and Mg almost complied with that in Knop's solution. In the variant P was 12-fold, S - 17-fold, K - 17-fold, Ca - 6-fold and Mg was 8-fold higher than in Knop's solution. The content of N and NaCl in both variants was the same and constituted 0.18 g/l and 10 g/l respectively. The results of carried experiments showed that growing plants on urine treated in the above-mentioned way is possible; though the productivity of plants would be less than on model solutions. The reasons of plant productivity drop and the possible ways of their removal have been discussed.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sarangova A.B., Somova L.A.
Заглавие : A new enzymatic technique to estimate the efficiency of microbial degradation of pollutants
Место публикации : Advances in Space Research. - 1997. - Vol. 20, Is. 10. - С. 2049-2052. - ISSN 02731177 (ISSN)
Ключевые слова (''Своб.индексиров.''): catalase--hydrogen peroxide--aerobic metabolism--article--bacterium--biomass--bioremediation--enzymology--metabolism--methodology--microbiology--sewage--waste management--water management--aerobiosis--bacteria--biodegradation, environmental--biomass--catalase--hydrogen peroxide--sewage--waste management--water microbiology--water purification
Аннотация: Dynamics of active sludge microorganism activity in aerotanks under chemostat conditions has been studied. Dependence of microorganism catalase activity has been found to depend on residual substrate concentration in proportion to the biomass of microorganisms. Experimental data and field observations has formed the basis to develop a technique to evaluate in relative units the amount of the substrate consumed by biocenosis of the active sludge in the air tanks of purification facilities. В© 1997 COSPAR. Published by Elsevier Science Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Biel K.Y., Fomina I.R., Nazarova G.N., Soukhovolsky V.G., Khlebopros R.G., Nishio J.N.
Заглавие : Untangling metabolic and spatial interactions of stress tolerance in plants. 1. Patterns of carbon metabolism within leaves
Место публикации : Protoplasma. - 2010. - Vol. 245, Is. 1. - С. 49-73. - ISSN 0033183X (ISSN) , DOI 10.1007/s00709-010-0135-7
Ключевые слова (''Своб.индексиров.''): carbon metabolism--leaf anatomy--leaf form and function--maximal ecological utility--photosynthesis--stress tolerance spinacia oleracea--aspartate aminotransferase isoenzyme 1--bicarbonate--carbon--carbon dioxide--catalase--chlorophyll--malate dehydrogenase--oxygen--ribulosebisphosphate carboxylase--vegetable protein--article--enzymology--histology--light--metabolism--oxidation reduction reaction--photosynthesis--physiological stress--physiology--plant leaf--spinach--theoretical model--aspartate aminotransferase, cytoplasmic--bicarbonates--carbon--carbon dioxide--catalase--chlorophyll--light--malate dehydrogenase--models, theoretical--oxidation-reduction--oxygen--photosynthesis--plant leaves--plant proteins--ribulose-bisphosphate carboxylase--spinacia oleracea--stress, physiological--spinacia oleracea
Аннотация: The localization of the key photoreductive and oxidative processes and some stress-protective reactions within leaves of mesophytic C3 plants were investigated. The role of light in determining the profile of Rubisco, glutamate oxaloacetate transaminase, catalase, fumarase, and cytochrome-c-oxidase across spinach leaves was examined by exposing leaves to illumination on either the adaxial or abaxial leaf surfaces. Oxygen evolution in fresh paradermal leaf sections and CO2 gas exchange in whole leaves under adaxial or abaxial illumination was also examined. The results showed that the palisade mesophyll is responsible for the midday depression of photosynthesis in spinach leaves. The photosynthetic apparatus was more sensitive to the light environment than the respiratory apparatus. Additionally, examination of the paradermal leaf sections by optical microscopy allowed us to describe two new types of parenchyma in spinach-pirum mesophyll and pillow spongy mesophyll. A hypothesis that oxaloacetate may protect the upper leaf tissue from the destructive influence of active oxygen is presented. The application of mathematical modeling shows that the pattern of enzymatic distribution across leaves abides by the principle of maximal ecological utility. Light regulation of carbon metabolism across leaves is discussed. В© 2010 Springer-Verlag.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondar V.S., Purtov K.V., Malikova N.P., Frank L.A., Illarionov B.A.
Заглавие : Role of conservative residue Cys158 in the formation of an active photoprotein complex of obelin
Колич.характеристики :5 с
Место публикации : Biochem.-Moscow: MAIK NAUKA/INTERPERIODICA, 2001. - Vol. 66, Is. 9. - С. 1014-1018. - ISSN 0006-2979, DOI 10.1023/A:1012377827626
Примечания : Cited References: 21
Предметные рубрики: CDNA
EXPRESSION
AEQUORIN
SEQUENCE
CLONING
Ключевые слова (''Своб.индексиров.''): photoproteins--obelin--apoobelin mutants--bioluminescence
Аннотация: Using site directed mutagenesis, the conservative residue Cys158 of recombinant apoobelin was substituted for sera ine (C158S, S-mutant) or alanine (C158A, A-mutant). These point mutations resulted in significant changes in the apoobelin structure accompanied by slowing of photoprotein complex formation, decrease of its stability, and changing of its bioluminescence characteristics. The enzymatic properties of the photoprotein decreased in the series: wild-type protein S-mutant A-mutant. This is consistent with rank of nucleophilicity SH OH CH3 of cysteine, serine, and alanine side chain functional groups, respectively. Possible mechanisms of the involvement of the apoobelin Cys158 SH-group in the formation of the enzyme-substrate complex are considered.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Rimatskaia N..., Baigina E..., Kazanceva M..., Stom D..., Kratasyuk V...
Заглавие : Application of bioluminescent enzymatic method for assessment of the state of the soil
Колич.характеристики :2 с
Место публикации : Luminescence: WILEY-BLACKWELL, 2014. - Vol. 29. - С. 76-77. - ISSN 1522-7235. - ISSN 1522-7243
Примечания : Cited References: 0
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Asanova A..., Esimbekova E..., Kratasyuk V...
Заглавие : Bioluminescent Enzymatic Methods for Toxicological Safety Testing of Food Additives
Колич.характеристики :1 с
Место публикации : Luminescence: WILEY-BLACKWELL, 2014. - Vol. 29. - С. 74-74. - ISSN 1522-7235. - ISSN 1522-7243
Примечания : Cited References: 4
Предметные рубрики: ASSAY
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Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Sarangova A.B., Somova L.A.
Заглавие : A new enzymatic technique to estimate the efficiency of microbial degradation of pollutants
Колич.характеристики :4 с
Место публикации : LIFE SCIENCES: LIFE SUPPORT SYSTEMS STUDIES-I. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON PRESS LTD, 1997. - Vol. 20: F4.6, F4.8, F4.2 and F4.9 Symposia of COSPAR Scientific Commission F on Life Sciences - Life Support System Studies-I, at the 31st COSPAR Scientific Assembly (JUL 14-SEP 21, 1996, BIRMINGHAM, ENGLAND), Is. 10. - P2049-2052. - ISBN 0273-1177, DOI 10.1016/S0273-1177(97)00940-X. - ISBN 0-08-043307-3
Примечания : Cited References: 4
Аннотация: Dynamics of active sludge microorganism activity in aerotanks under chemostat conditions has been studied. Dependence of microorganism catalase activity has been found to depend on residual substrate concentration in proportion to the biomass of microorganisms. Experimental data and field observations has formed the basis to develop a technique to evaluate in relative units the amount of the substrate consumed by biocenosis of the active sludge in the air tanks of purification facilities. (C) 1997 COSPAR. Published by Elsevier Science Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kudryasheva N.S., Kudinova I.Y., Esimbekova E.N., Kratasyuk V.A., Stom D.I.
Заглавие : The influence of quinones and phenols on the triple NAD(H)-dependent enzyme systems
Колич.характеристики :8 с
Место публикации : Chemosphere: PERGAMON-ELSEVIER SCIENCE LTD, 1999. - Vol. 38, Is. 4. - P751-758. - ISSN 0045-6535, DOI 10.1016/S0045-6535(98)00218-5
Примечания : Cited References: 7
Аннотация: Kinetics of the triple bioluminescent enzyme system: alcohol dehydrogenase - NADH:FMN-oxidoreductase - luciferase in the presence of quinones and phenols has been studied. The correspondence between the bioluminescent kinetic parameters, redox potentials and concentrations of the quinones and phenols has been estimated. The substances have been shown to change bioluminescent kinetics through moving off the NAD(+)/NADH balance in the enzyme processes. This system is proposed to be used as enzymatic biotest in ecological monitoring. (C) 1998 Elsevier Science Ltd. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kudryasheva N.S., Esimbekova E.N., Kudinova I.Y., Kratasyuk V.A., Stom D.I.
Заглавие : Effects of quinones on NADH-dependent enzymatic bioluminescent systems
Колич.характеристики :5 с
Место публикации : Appl. Biochem. Microbiol.: MAIK NAUKA/INTERPERIODICA, 2000. - Vol. 36, Is. 4. - P409-413. - ISSN 0003-6838, DOI 10.1007/BF02738052
Примечания : Cited References: 13
Аннотация: The effects of a number of quinones on the bioluminescence characteristics of a three-component enzymatic system containing alcohol dehydrogenase, bacterial luciferase, and NADH-FMN oxidoreductase were studied to find the most sensitive kinetic parameters of the system intended to be used in biological testing. Both direct and back reactions catalyzed by alcohol dehydrogenase were studied in the presence and in the absence of quinones. The kinetic parameters of the bioluminescent system were found to depend on the redox potentials and concentrations of quinones. The quinone-induced effects were shown to be associated with changes in the NAD(+)/NADH ratio in the chain of NADH-dependent enzymes, The three-enzyme system based on alcohol dehydrogenase is suggested as a bioluminescence test for ecological monitoring of waste water.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bauer S..., Schott A.K., Illarionova V..., Bacher A..., Huber R..., Fischer M...
Заглавие : Biosynthesis of tetrahydrofolate in plants: Crystal structure of 7,8-dihydroneopterin aldolase from Arabidopsis thaliana reveals a novel adolase class
Колич.характеристики :13 с
Место публикации : J. Mol. Biol.: ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD, 2004. - Vol. 339, Is. 4. - P967-979. - ISSN 0022-2836, DOI 10.1016/j.jmb.2004.04.034
Примечания : Cited References: 66
Предметные рубрики: GTP CYCLOHYDROLASE-I
GUANOSINE TRIPHOSPHATE CYCLOHYDROLASE
6-PYRUVOYL TETRAHYDROPTERIN SYNTHASE
ESCHERICHIA-COLI
DIHYDRONEOPTERIN ALDOLASE
FOLIC-ACID
ENZYMATIC SYNTHESIS
DIHYDROPTEROATE SYNTHASE
REACTION-MECHANISM
3-DIMENSIONAL STRUCTURE
Ключевые слова (''Своб.индексиров.''): tetrahydrofolate biosynthesis--aldolase classes--retroaldol reaction--purin binding--schiff base
Аннотация: Dihydroneopterin aldolase (DHNA) catalyses a retroaldol reaction yielding 6-hydroxymethyl-7,8-dihydropterin, a biosynthetic precursor of the vitamin, tetrahydrofolate. The enzyme is a potential target for antimicrobial and anti-parasite chemotherapy. A gene specifying a dihydroneopterin aldolase from Arabidopsis thaliana was expressed in a recombinant Escherichia coli strain. The recombinant protein was purified to apparent homogeneity and crystallised using polyethylenglycol as the precipitating agent. The crystal structure was solved by X-ray diffraction analysis at 2.2 Angstrom resolution. The enzyme forms a D-4-symmetric homo-octamer. Each polypeptide chain is folded into a single domain comprising an antiparallel four-stranded beta-sheet and two long alpha-helices. Four monomers are arranged in a tetrameric ring, and two of these rings form a hollow cylinder. Well defined purine derivatives are found at all eight topologically equivalent active sites. The subunit fold of the enzyme is related to substructures of dihydroneopterin triphosphate epimerase, GTP cyclohydrolase I, and pyruvoyltetrahydropterin synthase, which are all involved in the biosynthesis of pteridine type cofactors, and to urate oxidase, although some members of that superfamily have no detectable sequence similarity Due to structural and mechanistical differences of DHNA in comparison with class I and class II aldolases, a new aldolase class is proposed. (C) 2004 Elsevier Ltd. All rights reserved.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kudryasheva N.S., Tarasova A.S.
Заглавие : Pollutant toxicity and detoxification by humic substances: mechanisms and quantitative assessment via luminescent biomonitoring
Колич.характеристики :13 с
Коллективы : Russian Foundation for Basic Research [13-04-98072-sibir-a], RussianScience Foundation [14-14-00076]
Место публикации : Environ. Sci. Pollut. Res.: SPRINGER HEIDELBERG, 2015. - Vol. 22, Is. 1. - С. 155-167. - ISSN 0944-1344, DOI 10.1007/s11356-014-3459-6. - ISSN 1614-7499(eISSN)
Примечания : Cited References:120. - The work was supported by the Russian Foundation for Basic Research,Grant No. 13-04-98072-sibir-a. Part of the work (analysis ofdetoxification of radioactive solutions) was supported by the RussianScience Foundation, Grant No. 14-14-00076.
Предметные рубрики: PHOTOBACTERIUM-LEIOGNATHI LUCIFERASE
DISSOLVED ORGANIC-MATTER
Ключевые слова (''Своб.индексиров.''): detoxification mechanisms--humic substances--pollutants--bioassays--bioluminescence
Аннотация: The paper considers mechanisms of detoxification of pollutant solutions by water-soluble humic substances (HSs), natural detoxifying agents. The problems and perspectives of bioassay application for toxicity monitoring of complex solutions are discussed from ecological point of view. Bioluminescence assays based on marine bacteria and their enzymes are of special attention here; they were shown to be convenient tools to study the detoxifying effects on cellular and biochemical levels. The advantages of bioluminescent enzymatic assay for monitoring both integral and oxidative toxicities in complex solutions of model pollutants and HS were demonstrated. The efficiencies of detoxification of the solutions of organic oxidizers and salts of metals (including radioactive ones) by HS were analyzed. The dependencies of detoxification efficiency on time of exposure to HS and HS concentrations were demonstrated. Antioxidant properties of HS were considered in detail. The detoxifying effects of HS were shown to be complex and regarded as 'external' (binding and redox processes in solutions outside the organisms) and/or 'internal' organismal processes. The paper demonstrates that the HS can stimulate a protective response of bacterial cells as a result of (1) changes of rates of biochemical reactions and (2) stabilization of mucous layers outside the cell walls. Acceleration of auto-oxidation of NADH, endogenous reducer, by HS was suggested as a reason for toxicity increase in the presence of HS due to abatement of reduction ability of intracellular media.
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12.

Вид документа : Статья из сборника (выпуск монографической серии)
Шифр издания :
Автор(ы) : Esimbekova, Elena, Kratasyuk, Valentina, Shimomura, Osamu
Заглавие : Application of Enzyme Bioluminescence in Ecology
Колич.характеристики :43 с
Место публикации : Adv. Biochem. Eng. Biotechnol.: SPRINGER-VERLAG BERLIN, 2014. - Vol. 144. - С. 67-109. - (Advances in Biochemical Engineering-Biotechnology). - , DOI 10.1007/978-3-662-43385-0_3
Примечания : Cited References:85
Предметные рубрики: BACTERIAL LUCIFERASE
IN-VITRO
PYRETHROID INSECTICIDES
FRESH-WATER
Ключевые слова (''Своб.индексиров.''): bioluminescence--ecological monitoring--enzymatic assay--immobilization--integral water toxicity--luciferase
Аннотация: This review examines the general principles of bioluminescent enzymatic toxicity bioassays and describes the applications of these methods and the implementation in commercial biosensors. Bioluminescent enzyme system technology (BEST) has been proposed in the bacterial coupled enzyme system, wherein NADH: FMN-oxidoreductase-luciferase substitutes for living organisms. BEST was introduced to facilitate and accelerate the development of cost-competitive enzymatic systems for use in biosensors for medical, environmental, and industrial applications. For widespread use of BEST, the multicomponent reagent "Enzymolum'' has been developed, which contains the bacterial luciferase, NADH: FMN-oxidoreductase, and their substrates, co-immobilized in starch or gelatin gel. Enzymolum is the central part of Portable Laboratory for Toxicity Detection (PLTD), which consists of a biodetector module, a sampling module, a sample preparation module, and a reagent module. PLTD instantly signals chemical-biological hazards and allows us to detect a wide range of toxic substances. Enzymolum can be integrated as a biological module into the portable biodetector-biosensor originally constructed for personal use. Based on the example of Enzymolum and the algorithm for creating new enzyme biotests with tailored characteristics, a new approach was demonstrated in biotechnological design and construction. The examples of biotechnological design of various bioluminescent methods for ecological monitoring were provided. Possible applications of enzyme bioassays are seen in the examples for medical diagnostics, assessment of the effect of physical load on sportsmen, analysis of food additives, and in practical courses for higher educational institutions and schools. The advantages of enzymatic assays are their rapidity (the period of time required does not exceed 3-5 min), high sensitivity, simplicity and safety of procedure, and possibility of automation of ecological monitoring; the required luminometer is easily available.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tarasova A.S., Stom D.I., Kudryasheva N.S.
Заглавие : Antioxidant activity of humic substances via bioluminescent monitoring in vitro
Колич.характеристики :8 с
Коллективы : Russian Foundation for Basic Research [15-03-06786a], Russian Academy of Sciences [VI 57.1.1]
Место публикации : Environ. Monit. Assess.: SPRINGER, 2015. - Vol. 187, Is. 3. - Ст.89. - ISSN 0167-6369, DOI 10.1007/s10661-015-4304-1. - ISSN 1573-2959(eISSN)
Примечания : Cited References:51. - This work was supported by the Russian Foundation for Basic Research, Grant No. 15-03-06786a, the Program "Molecular and Cellular Biology" of the Russian Academy of Sciences, project VI 57.1.1.
Предметные рубрики: DETOXIFICATION PROCESSES
TOXICITY
BIOASSAYS
BACTERIA
ASSAY
Ключевые слова (''Своб.индексиров.''): antioxidant activity--oxidative toxicity--general toxicity--humic--substances--bioassay--bioluminescence
Аннотация: This work considers antioxidant properties of natural detoxifying agents-humic substances (HS) in solutions of model inorganic and organic compounds of oxidative nature-complex salt K-3[Fe(CN)(6)] and 1,4-benzoquinone. Bioluminescent system of coupled enzymatic reactions catalyzed by NAD(P) H:FMN-oxidoreductase and bacterial luciferase was used as a bioassay in vitro to monitor toxicity of the oxidizer solutions. Toxicities of general and oxidative types were evaluated using bioluminescent kinetic parameters-bioluminescence intensity and induction period, respectively. Antioxidant activity of HS was attributed to their ability to decrease both general and oxidative toxicities; the HS antioxidant efficiency was characterized with detoxification coefficients D-GT and D-OxT, respectively. Dependencies of D-GT and D-OxT on HS concentration and time of preliminary incubation of the oxidizers with HS were demonstrated. The optimal conditions for detoxification of the oxidizers were 20-min incubation time and 0.5x10(-4) to 2x10(-4) M of HS concentration. The present study promotes application of the enzymatic luminescent bioassay to monitor toxicity of pollutants of oxidative nature in environmental and waste waters in remediation procedures.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Esimbekova, E. N., Lonshakova-Mukina, V. I., Bezrukikh, A. E., Kratasyuk, V. A.
Заглавие : Design of multicomponent reagents for enzymatic assays
Колич.характеристики :4 с
Коллективы : Program of the Russian Academy of Sciences "Molecular and Cell Biology" [6.8], Ministry of Education and Science of the Russian Federation; Siberian Federal University [1762]
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2015. - Vol. 461, Is. 1. - С. 102-105. - ISSN 1607-6729, DOI 10.1134/S1607672915020106. - ISSN 1608-3091(eISSN)
Примечания : Cited References:8. - The work was supported by the Program of the Russian Academy of Sciences "Molecular and Cell Biology" (project no. 6.8) as well as within the state contract between the Ministry of Education and Science of the Russian Federation and Siberian Federal University (project 1762).
Предметные рубрики: BIOLUMINESCENT
WATER
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kratasyuk, Valentina A., Esimbekova, Elena N.
Заглавие : Applications of Luminous Bacteria Enzymes in Toxicology
Колич.характеристики :8 с
Коллективы : Russian Science Foundation [15-19-10041]
Место публикации : Comb. Chem. High Throughput Screen: BENTHAM SCIENCE PUBL LTD, 2015. - Vol. 18, Is. 10. - С. 952-959. - ISSN 1386-2073, DOI 10.2174/1386207318666150917100257. - ISSN 1875-5402(eISSN)
Примечания : Cited References:88. - The research was supported by the Russian Science Foundation, project No. 15-19-10041.
Предметные рубрики: NADHFMN-OXIDOREDUCTASE-LUCIFERASE
HUMIC SUBSTANCES
BIOLUMINESCENT
Ключевые слова (''Своб.индексиров.''): bioluminescence--bioluminescent toxicity enzymatic assay--immobilization--of enzymes--luciferase--total toxicity
Аннотация: This review describes the principle and applications of bioluminescent enzymatic toxicity bioassays. This type of assays uses bacterial coupled enzyme systems: NADH: FMN-oxidoreductase and luciferase to replace living organisms in developing cost-competitive biosensors for environmental, medical and industrial applications. These biosensors instantly signal chemical and biological hazards and allow for detecting a great amount of toxic compounds with advantages associated with fast results, high sensitivity, simplicity, low cost and safety of the procedure.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kratasyuk V. A., Esimbekova E. N.
Заглавие : Applications of luminous bacteria enzymes in toxicology
Место публикации : Comb. Chem. High Throughput Screen. - 2015. - Vol. 18, Is. 10. - С. 952-959. - ISSN 13862073 (ISSN)
Ключевые слова (''Своб.индексиров.''): bioluminescence--bioluminescent toxicity enzymatic assay--immobilization of enzymes--luciferase--total toxicity
Аннотация: This review describes the principle and applications of bioluminescent enzymatic toxicity bioassays. This type of assays uses bacterial coupled enzyme systems: NADH:FMN-oxidoreductase and luciferase to replace living organisms in developing cost-competitive biosensors for environmental, medical and industrial applications. These biosensors instantly signal chemical and biological hazards and allow for detecting a great amount of toxic compounds with advantages associated with fast results, high sensitivity, simplicity, low cost and safety of the procedure. © 2015 Bentham Science Publishers.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Deeva, Anna A., Temlyakova, Evgenia A., Sorokin, Anatoly A., Nemtseva, Elena V., Kratasyuk, Valentina A.
Заглавие : Structural distinctions of fast and slow bacterial luciferases revealed by phylogenetic analysis
Колич.характеристики :5 с
Коллективы : RFBR [16-34-00746 mol_a]; Ministry of Education and Science of the Russian Federation [1762]; state budget allocated to the fundamental research at Russian Academy of Sciences [01201351504]
Место публикации : Bioinformatics: OXFORD UNIV PRESS, 2016. - Vol. 32, Is. 20. - С. 3053-3057. - ISSN 1367-4803, DOI 10.1093/bioinformatics/btw386. - ISSN 1460-2059(eISSN)
Примечания : Cited References:31. - The reported study was partially funded by RFBR according to the research project No. 16-34-00746 mol_a; by the Ministry of Education and Science of the Russian Federation [project No 1762] and by the state budget allocated to the fundamental research at the Russian Academy of Sciences [project No 01201351504].
Аннотация: Motivation: Bacterial luciferases are heterodimeric enzymes that catalyze a chemical reaction, so called bioluminescence, which causes light emission in bacteria. Bioluminescence is vastly used as a reporter system in research tools and commercial developments. However, the details of the mechanisms that stabilize and transform the reaction intermediates as well as differences in the enzymatic kinetics amongst different bacterial luciferases remain to be elucidated. Results: Amino acid sequences alignments for 21 bacterial luciferases (both alpha- and beta-subunits) were analyzed. For alpha-subunit, containing the enzyme active center, 48 polymorphic amino acid positions were identified. According to them, the sequences fell into two distinct groups known as slow and fast based on the decay rate of the bioluminescence reaction. The differences in the enzyme active site induced by structural polymorphism are analyzed.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sachkova A. S., Kovel E. S., Churilov G. N., Guseynov O. A., Bondar A. A., Dubinina I. A., Kudryasheva N. S.
Заглавие : On mechanism of antioxidant effect of fullerenols
Место публикации : Biochem. Biophys. Rep.: Elsevier B.V., 2017. - Vol. 9. - С. 1-8. - ISSN 24055808 (ISSN) , DOI 10.1016/j.bbrep.2016.10.011
Ключевые слова (''Своб.индексиров.''): antioxidant activity--bacterial enzymes--fullerenol--hormesis--luminous marine bacteria--ultralow concentrations
Аннотация: Fullerenols are nanosized water-soluble polyhydroxylated derivatives of fullerenes, specific allotropic form of carbon, bioactive compounds and perspective pharmaceutical agents. Antioxidant activity of fullerenols was studied in model solutions of organic and inorganic toxicants of oxidative type – 1,4-benzoquinone and potassium ferricyanide. Two fullerenol preparations were tested: С60О2–4(ОН)20–24 and mixture of two types of fullerenols С60О2–4(ОН)20–24+С70О2–4(ОН)20–24. Bacteria-based and enzyme-based bioluminescent assays were used to evaluate a decrease in cellular and biochemical toxicities, respectively. Additionally, the enzyme-based assay was used for the direct monitoring of efficiency of the oxidative enzymatic processes. The bacteria-based and enzyme-based assays showed similar peculiarities of the detoxification processes: (1) ultralow concentrations of fullerenols were active (ca 10–17–10?4 and 10–17–10? 5 g/L, respectively), (2) no monotonic dependence of detoxification efficiency on fullerenol concentrations was observed, and (3) detoxification of organic oxidizer solutions was more effective than that of the inorganic oxidizer. The antioxidant effect of highly diluted fullerenol solutions on bacterial cells was attributed to hormesis phenomenon; the detoxification was concerned with stimulation of adaptive cellular response under low-dose exposures. Sequence analysis of 16S ribosomal RNA was carried out; it did not reveal mutations in bacterial DNA. The suggestion was made that hydrophobic membrane-dependent processes are involved to the detoxifying mechanism. Catalytic activity of fullerenol (10? 8 g/L) in NADH-dependent enzymatic reactions was demonstrated and supposed to contribute to adaptive bacterial response. © 2016 The Authors
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kudryasheva, Nadezhda S., Kovel, Ekaterina S., Sachkova, Anna S., Vorobeva, Anna A., Isakova, Viktoriya G., Churilov, Grigoriy N.
Заглавие : Bioluminescent Enzymatic Assay as a Tool for Studying Antioxidant Activity and Toxicity of Bioactive Compounds
Колич.характеристики :5 с
Коллективы : Russian Foundation for Basic Research [15-03-06786, 15-43-04377-sibir]; Russian Academy of Sciences [01201351504]
Место публикации : Photochem. Photobiol.: WILEY, 2017. - Vol. 93, Is. 2. - С. 536-540. - ISSN 0031-8655, DOI 10.1111/php.12639. - ISSN 1751-1097(eISSN)
Примечания : Cited References:40. - The work was supported by the Russian Foundation for Basic Research, Grants 15-03-06786 and 15-43-04377-sibir; the state budget allocated to the fundamental research at the Russian Academy of Sciences (project 01201351504).
Предметные рубрики: LUMINOUS MARINE-BACTERIA
HUMIC SUBSTANCES
DETOXIFICATION PROCESSES
Аннотация: A bioluminescent assay based on a system of coupled enzymatic reactions catalyzed by bacterial luciferase and NADH:FMN-oxidoreductase was developed to monitor toxicity and antioxidant activity of bioactive compounds. The assay enables studying toxic effects at the level of biomolecules and physicochemical processes, as well as determining the toxicity of general and oxidative types. Toxic and detoxifying effects of bioactive compounds were studied. Fullerenols, perspective pharmaceutical agents, nanosized particles, water-soluble polyhydroxylated fullerene-60 derivatives were chosen as bioactive compounds. Two homologous fullerenols with different number and type of substituents, C60O2-4(OH)(20-24) and Fe0.5C60(OH) O-x(y) (x + y = 40-42), were used. They suppressed bioluminescent intensity at concentrations 0.01 g L-1 and 0.001 g L-1 for C60O2-4(OH)(20-24) and Fe0.5C60(OH)(x)O-y, respectively; hence, a lower toxicity of C60O2-4(OH)(20-24) was demonstrated. Antioxidant activity of fullerenols was studied in model solutions of organic and inorganic oxidizers; changes in toxicities of general and oxidative type were determined; detoxification coefficients were calculated. Fullerenol C60O2-4(OH)(20-24) revealed higher antioxidant ability at concentrations 10(-17)-10(-5) g L-1. The difference in the toxicity and antioxidant activity of fullerenols was explained through their electron donor/acceptor properties and different catalytic activity. Principles of bioluminescent enzyme assay application for evaluating the toxic effect and antioxidant activity of bioactive compounds were summarized and the procedure steps were described.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Lukyanenko, Kirill A., Belousov, Kirill I., Denisov, Ivan A., Yakimov, Anton S., Esimbekova, Elena N., Bukatin, Anton S., Evstrapov, Anatoly A., Belobrov, Peter I.
Заглавие : Active mixing of immobilised enzymatic system in microfluidic chip
Колич.характеристики :5 с
Коллективы : Russian Science Foundation [15-19-10041]
Место публикации : Micro Nano Lett.: INST ENGINEERING TECHNOLOGY-IET, 2017. - Vol. 12, Is. 6. - С. 377-381. - ISSN 1750-0443, DOI 10.1049/mnl.2016.0646
Примечания : Cited References:17. - The research was supported by the grant of the Russian Science Foundation (project no. 15-19-10041).
Предметные рубрики: POLY(METHYL METHACRYLATE)
SURFACE MODIFICATION
POINT
DEVICES
PMMA
Аннотация: Parameters for sample introduction, dried reagents dissolution and mixing with sample for bienzyme system NAD(H):FMN-oxidoreductase and luciferase immobilised in microfluidic chip were successfully determined. Numerical simulations of reaction chamber geometry, flavin mononucleotide (FMN) escape from starch gel and mixing options were conducted to achieve higher sensitivity of bioluminescent reaction. Results of numerical simulations were verified experimentally. The active mixer for dried reagents was made from an electro-mechanical speaker's membrane which was connected to the input of the chip. Such a mixer provided better efficiency than a passive mixing, and it is simple enough for use in point-of-care devices with any systems based on immobilised enzymes in chips.
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