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A low-cost underwater particle tracking velocimetry system for measuring in situ particle flux and sedimentation rate in low-turbulence environments / S. Simoncelli [et al.] // Limnol. Oceanogr. Methods. - 2019, DOI 10.1002/lom3.10341 . - Article in press. - ISSN 1541-5856
Аннотация: We describe a low-cost three-dimensional underwater particle tracking velocimetry system to directly measure particle settling rate and flux in low-turbulence aquatic environments. The system consists of two waterproof cameras that acquire stereoscopic videos of sinking particles at 48 frames s?1 over a tunable sampling volume of about 45 ? 25 ? 24 cm. A dedicated software package has been developed to allow evaluation of particle velocities, concentration and flux, but also of morphometric parameters such as particle area, sinking angle, shape irregularity, and density. Our method offers several advantages over traditional approaches, like sediment trap or expensive in situ camera systems: (1) it does not require beforehand particle collection and handling; (2) it is not subjected to sediment trap biases from turbulence, horizontal advection, or presence of swimmers, that may alter particulate load and flux; (3) the camera system enables faster data processing and flux computation at higher spatial resolution; (4) apart from the particle settling rates, the particle size distribution, and morphology is determined. We tested the camera system in Lake Stechlin (Germany) in low turbulence and mean flow, and analyzed the morphological properties and settling rates of particles to determine their sinking behavior. The particle flux assessed from conventional sediment trap measurements agreed well with that determined by our system. By this, the low-cost approach demonstrated its reliability in low turbulence environments and a strong potential to provide new insights into particulate carbon transport in aquatic systems. Extension of the method to more turbulent and advective conditions is also discussed. © 2019 The Authors. Limnology and Oceanography: Methods published by Wiley Periodicals, Inc. on behalf of Association for the Sciences of Limnology and Oceanography.

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Держатели документа:
Leibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB), Berlin, Germany
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Potsdam University, Institute for Biochemistry and Biology, Potsdam, Germany

Доп.точки доступа:
Simoncelli, S.; Kirillin, G.; Tolomeev, A. P.; Grossart, H. -P.

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An optimization model of competition between species for resource. / V. G. Sukhovol'skii, R. G. Khlebopros, T. R. Iskhakov // Doklady. Biochemistry and biophysics. - 2003. - Vol. 390. - P171-173 . - ISSN 1607-6729
Кл.слова (ненормированные):
animal -- article -- biological model -- comparative study -- competitive behavior -- computer simulation -- ecosystem -- evaluation -- growth, development and aging -- insect -- physiology -- plankton -- population dynamics -- population growth -- quality control -- species difference -- validation study -- Animals -- Competitive Behavior -- Computer Simulation -- Ecosystem -- Insects -- Models, Biological -- Plankton -- Population Dynamics -- Population Growth -- Quality Control -- Species Specificity

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Держатели документа:
Sukachev Institute of Forest, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sukhovol'skii, V.G.; Khlebopros, R.G.; Iskhakov, T.R.

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Analysis of the variability of temperature gradient in the ocean frontal zones based on satellite data / A. V. Kartushinsky, A. Y. Sidorenko // Advances in Space Research. - 2013, DOI 10.1016/j.asr.2013.07.023 . - ISSN 0273-1177
Кл.слова (ненормированные):
Frontal zones -- North Atlantic oscillation -- Sea surface temperature gradients -- South oscillation - El Ninjo -- Thermohaline circulation -- Variability of gradients
Аннотация: AVHRR MCSST data for the periods 1982-2000 (mean weekly data) were used to calculate mean gradient fields in the ocean for different periods of time. Three-month averaged sea surface temperature gradients (SSTG) and their mean seasonal variations have been studied for 25 points in the large-scale oceanic fronts zones. Major oceanic fronts in the Atlantic and Pacific have been identified and compared in literature. In the North Atlantic and Pacific, the areas under study were the North Polar Front and Subpolar Fronts. In the South Atlantic and Pacific we studied the region of the Antarctic Circumpolar Current (ACC) and the fronts formed by this current, known as the South Polar Front, and the Subantarctic Front. SSTG were also calculated for El Nino (Southeast Pacific) and Benguela Current (Southeast Atlantic). In warm periods seasonal SSTG in the North Atlantic markedly increased and exhibit some interannual cycles. The correlation between the North Atlantic Oscillation index and seasonal SSTG for a single point in the Gulf Stream zone can be the key point for evaluation of heat transfer by the currents to the coast of East Europe. In the Southern Atlantic, the SSTG values are low during the cold period (summer in the southern hemisphere) in the ACC zone and increase in the warm season (winter in the southern hemisphere). It also exhibits interannual cycles. In the Northwest Pacific for some points in the Subpolar Front the SSTG values are high in the cold period (winter). Here at seven points in the spring of 1993 and 2000 the calculations disclosed significant increase of the gradient. In these years, the anomalous SSTG in Subpolar Front and South Polar Front were found to vary synchronously in both hemispheres, with maximum intensity in spring (North Pacific) and in summer (South Pacific). Mean annual SSTG in the El Nino zone and south oscillation index have been found to exhibit some correlation. Major jet currents periodically form high-gradient temperature fields and from the temperature satellite data we can derive information about variation in the large-scale fronts in the Global Ocean. В© 2013 COSPAR.

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Держатели документа:
Siberian Federal University, 79 Svobodny, Krasnoyarsk 660041, Russia
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Akademgorodok, Krasnoyarsk 660036, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kartushinsky, A.V.; Sidorenko, A.Y.

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Application of satellite data for investigation of dynamic processes in inland water bodies: Lake Shira (Khakasia, Siberia), a case study / A. P. Shevyrnogov, A. V. Kartushinsky, G. S. Vysotskaya // Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - P153-163, DOI 10.1023/A:1015658927683 . - ISSN 1386-2588
Кл.слова (ненормированные):
Modelling -- Phytopigments -- Satellite data -- Satellite equipment -- Software -- Temperature -- AVHRR -- hydrodynamics -- lake -- limnology -- remote sensing -- saline lake -- satellite data -- water temperature -- Russian Federation
Аннотация: This work describes avenues to use satellite information to analyse dynamic processes in aquatic ecosystems. Information for this analysis, was retrieved from AVHRR satellite sensor data. This information consisteds of time series of images of radiation temperature and turbidity. We expect this information will be of great value in analysing inland water bodies. Methods to process satellite information using original software and data processing techniques are proposed. For the investigation of the process and analyses of satellite information Shira Lake (Khakasia, Siberia) was used as a case study. To study the variability of the surface temperature and turbidity of the Lake in summer, the satellite and ground-truth data of the lake was applied. This study represents the first evaluation of the dynamic processes for Lake Shira based on satellite, ground-truth and modelling data. We developed algorithms and software to process satellite images to enable the reconstruction of time dependence of temperature and spectral reflectance of water bodies in the visible range, and to make computer-animated films visualising the spatial and temporal dynamics of the study parameters. The analyses of morphometric, meteorological and hydrological characteristics of Lake Shira have provided a realistic opportunity for processing the satellite information and to develop numerical models of variability of the hydrological regime of the lake. The results obtained demonstrate the feasibility of systematically retrieving the spatial information from the satellite data on the dynamics of the surface water temperature and of the suspended matter in the lake.

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Держатели документа:
Institute of Biophysics of SB RAS, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shevyrnogov, A.P.; Kartushinsky, A.V.; Vysotskaya, G.S.

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Biocompatibility of polyhydroxybutyrate microspheres: In vitro and in vivo evaluation / E. I. Shishatskaya [et al.] // Journal of Materials Science: Materials in Medicine. - 2008. - Vol. 19, Is. 6. - P2493-2502, DOI 10.1007/s10856-007-3345-6 . - ISSN 0957-4530
Кл.слова (ненормированные):
Biocompatibility -- Drug delivery -- Fibroblasts -- Ion implantation -- Microspheres -- Polymer matrix -- Fibroblast cells -- Hydroxybutyric acid -- Intramuscular implantation -- Polyhydroxybutyrate -- Organic polymers -- microsphere -- poly(3 hydroxybutyric acid) -- polymer -- animal cell -- animal experiment -- animal tissue -- article -- biocompatibility -- cell infiltration -- controlled study -- giant cell -- implantation -- in vitro study -- in vivo study -- inflammation -- macrophage -- mouse -- nonhuman -- priority journal -- rat -- 3-Hydroxybutyric Acid -- Animals -- Biocompatible Materials -- Cell Survival -- Inflammation -- Materials Testing -- Mice -- Microspheres -- Necrosis -- NIH 3T3 Cells -- Polymers -- Rats -- Rats, Wistar -- Tetrazolium Salts -- Thiazoles -- Time Factors -- Rattus norvegicus
Аннотация: Microspheres have been prepared from the resorbable linear polyester of ?-hydroxybutyric acid (polyhydroxybutyrate, PHB) by the solvent evaporation technique and investigated in vitro and in vivo. Biocompatibility of the microspheres has been proved in tests in the culture of mouse fibroblast cell line NIH 3T3 and in experiments on intramuscular implantation of the microspheres to Wistar rats for 3 months. Tissue response to the implantation of polymeric microspheres has been found to consist in a mild inflammatory reaction, pronounced macrophage infiltration that increases over time, involving mono- and poly-nuclear foreign body giant cells that resorb the polymeric matrix. No fibrous capsules were formed around polymeric microparticles; neither necrosis nor any other adverse morphological changes and tissue transformation in response to the implantation of the PHB microparticles were recorded. The results of the study suggest that polyhydroxybutyrate is a good candidate for fabricating prolonged-action drugs in the form of microparticles intended for intramuscular injection. В© 2008 Springer Science+Business Media, LLC.

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Держатели документа:
Laboratory of Chemoautotrophic Biosynthesis, Institute of Biophysics SB RAS (Siberian Branch Russian Academy of Sciences), Akademgorodok, 50, Krasnoyarsk 660036, Russian Federation
Laboratory of Bacterial Bioluminescence, Institute of Biophysics SB RAS (Siberian Branch Russian Academy of Sciences), Akademgorodok, 50, Krasnoyarsk 660036, Russian Federation
Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Svo Bodnyi Av. 79, Krasnoyarsk 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Voinova, O.N.; Goreva, A.V.; Mogilnaya, O.A.; Volova, T.G.

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Biodegradable polymers - Perspectives and applications in agriculture / E. G. Kiselev, N. O. Zhila, T. G. Volova // IOP Conference Series: Earth and Environmental Science : IOP Publishing Ltd, 2021. - Vol. 689: 2020 International Conference on Germany and Russia: Ecosystems Without Borders, EcoSystConfKlgtu 2020 (5 October 2020 through 10 October 2020, ) Conference code: 167944, Is. 1. - Ст. 012036, DOI 10.1088/1755-1315/689/1/012036
Кл.слова (ненормированные):
Biodegradable polymers -- Ecosystems -- Fungi -- Glycerol -- Monounsaturated fatty acids -- Oilseeds -- Pesticides -- Substrates -- Sunflower oil -- Fenoxaprop-p-ethyl -- Natural materials -- Pesticide formulations -- Poly-3-hydroxybutyrate -- Polyhydroxyalkanoates -- Productive process -- Strategy of constructions -- Various substrates -- Palm oil
Аннотация: The paper presents a brief overview of the results of the implementation of the project "Agropreparations of the new generation: a strategy of construction and realization". The first part contains the analysis of the growth of the wild-type strain Cupriavidus necator B-10646 (formerly eutrophus) and the synthesis of polyhydroxyalkanoates by this strain on various substrates: glycerol, palm oil, Siberian oil seed, sunflower seed oils, and oleic acid. On refined glycerin, a highly productive process is implemented when scaling up, allowing to obtain 128 ± 11 g / L PHA. Evaluation of oils has shown that palm oil is the best carbon substrate. The second part presents the results of the development of environmentally friendly slow-release pesticide formulations. They are a degradable matrix of poly-3-hydroxybutyrate mixed with natural materials (peat, clay, wood flour), into which a pesticide (metribuzin, tribenuron-methyl, fenoxaprop-P-ethyl, azoxystrobin, epoxiconazole, and tebuconazole) has been. The developed preparations showed high activity against pathogenic fungi and weeds and had a much weaker negative effect on the soil microflora. Studies of the degradation of the developed preparations and the release of pesticides into the soil confirm their effectiveness over a long period of time, up to 90 days. © Published under licence by IOP Publishing Ltd.

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Держатели документа:
School of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation
Laboratory of Chemoautotrophic Biosynthesis, Institute of Biophysics, SB, RAS, Federal Research Center, Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Kiselev, E. G.; Zhila, N. O.; Volova, T. G.

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BIOLUMBASE - The database of natural and transgenic bioluminescent organisms / S. E. Medvedeva [et al.] // Luminescence. - 2005. - Vol. 20, Is. 2. - P90-96, DOI 10.1002/bio.809 . - ISSN 1522-7235
Кл.слова (ненормированные):
Bioluminescence -- Database -- Luminous bacteria -- lux gene -- Marine -- article -- bacterial strain -- bacterium culture -- bacterium isolation -- bioluminescence -- data base -- gene construct -- medical information -- transgenics -- wide area network -- Bacteria -- Bacterial Proteins -- Databases, Factual -- Ecology -- Luminescence -- Luminescent Proteins -- Marine Biology -- Organisms, Genetically Modified -- Photobacterium -- Transgenes
Аннотация: The Institute of Biophysics SB RAS hosts and maintains a specialized collection of luminous bacteria (CCIBSO 836) containing over 700 strains isolated in various regions of the world's oceans. The culture collection is a source of lux genes and biologically active substances. The wide application of bioluminescence in medicine and ecology has given importance to analys-ing information on the structure and functioning of bioluminescence systems in natural and transgenic microorganisms, as well as on their features that are closely interrelated with bioluminescence. The aims of our BIOLUMBASE database are: gathering information on microorganisms with lux genes, their analysis and free access, and distribution of this data throughout the global network. The database includes two sections, natural and transgenic luminous microorganisms, and is updated by our own experimental results, the published literature and internet resources. For the future, a publicly available internet site for BIOLUMBASE is planned. This will list the strains and provide comprehensive information on the properties and functions of luminous bacteria, the mechanisms of regulation of bioluminescence systems, constructs with lux genes, and applications of bioluminescence in microbiology, ecology, medicine and biotechnology. It is noteworthy that this database will also be useful for evaluation of biological hazards of transgenic strains. Users will be able to carry out bibliographic and strain searches starting from any feature of interest. Copyright В© 2005 John Wiley & Sons, Ltd.

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Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Medvedeva, S.E.; Boyandin, A.; Lankin, Y.; Kotov, D.; Rodicheva, E.; Popova, L.

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Bioluminescent analysis of intensity of pathological oxidative processes in cells of perfused rat liver after hyperthermia [Text] / N. N. Remmel [et al.] // Bull. Exp. Biol. Med. - 2003. - Vol. 135, Is. 1. - P. 43-45, DOI 10.1023/A:1023489727703. - Cited References: 15 . - ISSN 0007-4888

РУБ Medicine, Research & Experimental

Кл.слова (ненормированные):
bioluminescent analysis -- controlled perfusion of isolated liver -- hyperthermia -- oxidative stress
Аннотация: We studied the possibility of evaluation of the intensity of pathological oxidative processes in rat liver using an integral bioluminescent test with controlled perfusion of the isolated organ. The test revealed a significant correlation between the level of TBA-reactive products and bioluminescence intensity.

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Держатели документа:
Russian Acad Sci, Siberian Div, Inst Biophys, Moscow 117901, Russia
Krasnoyarsk State Univ, Krasnoyarsk, Russia
Russian Acad Sci, Siberian Div, Presidium Krasnoyarsk Res Ctr, Int Res Ctr Extreme States, Moscow 117901, Russia
ИБФ СО РАН
МНЦ КНЦ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Remmel, N.N.; Kratasyuk, V.A.; Maznyak, O.M.; Inzhevatkin, E.V.; Nefedov, V.P.

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Bioluminescent analysis of intensity of pathological oxidative processes in cells of perfused rat liver after hyperthermia / N. N. Remmel [et al.] // Bulletin of Experimental Biology and Medicine. - 2003. - Vol. 135, Is. 1. - P. 43-45, DOI 10.1023/A:1023489727703 . - ISSN 0007-4888
Кл.слова (ненормированные):
Bioluminescent analysis -- Controlled perfusion of isolated liver -- Hyperthermia oxidative stress -- thiobarbituric acid reactive substance -- animal experiment -- animal model -- animal tissue -- article -- bioluminescence -- concentration (parameters) -- controlled study -- correlation analysis -- female -- hyperthermia -- in vivo study -- lipid peroxidation -- liver metabolism -- liver perfusion -- male -- nonhuman -- oxidative stress -- oxygen consumption -- pathology -- rat -- thermal exposure -- Animals -- Female -- Hepatocytes -- Hyperthermia, Induced -- Liver -- Luminescent Measurements -- Male -- Oxidative Stress -- Perfusion -- Rats -- Animalia
Аннотация: We studied the possibility of evaluation of the intensity of pathological oxidative processes in rat liver using an integral bioluminescent test with controlled perfusion of the isolated organ. The test revealed a significant correlation between the level of TBA-reactive products and bioluminescence intensity.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Novosibirsk, Russian Federation
Krasnoyarsk State University, Krasnoyarsk, Russian Federation
Intl. Res. Ctr. of Extreme States, Siberian Division, Russian Academy of Sciences, Novosibirsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Remmel, N.N.; Kratasyuk, V.A.; Maznyak, O.M.; Inzhevatkin, E.V.; Nefedov, V.P.

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10.

Bioluminescent SNP genotyping technique: Development and application for detection of melanocortin 1 receptor gene polymorphisms / E. E. Bashmakova [et al.] // Talanta. - 2018. - Vol. 189. - P111-115, DOI 10.1016/j.talanta.2018.06.057 . - ISSN 0039-9140
Кл.слова (ненормированные):
Ca2+-regulated photoprotein obelin -- Genotyping -- Melanocortin 1 receptor gene -- Single nucleotide polymorphisms (SNP) -- Bioluminescence -- Clinical research -- Curricula -- Diagnosis -- Genes -- Oncology -- Biomedical research -- Clinical characteristics -- Development and applications -- Genotyping -- Healthy individuals -- Photoproteins -- Receptor genes -- Single-nucleotide polymorphisms -- Dermatology
Аннотация: SNP genotyping based on the reaction of specific primer extension with the following bioluminescent detection of its products was shown to be potentially applicable for biomedical exploration. The paper describes its elaboration and first application in extensive biomedical research concerning MC1R gene variants’ frequency and associations with clinical characteristics in melanoma patients of Eastern Siberia (Krasnoyarsk region, Russia). Polymorphisms rs 1805007 (R151C), rs 1805008 (R160W), and rs 1805009 (D294H) were detected in 174 DNA samples from patients with histologically proved diagnosis of cutaneous melanoma and in 200 samples from healthy individuals. All the results on bioluminescent SNP genotyping were confirmed by Sanger sequencing. Some features characteristic of the population were found, i.e. melanoma is mostly associated with R160W or R151C while variant D294H is extremely rare; simultaneous carriage of any two investigated variants is also strongly associated with melanoma; R151C is associated with ulceration and consequently the disease course is more aggressive, etc. The design of the technique allows fast evaluation of any known diagnostically important SNP frequencies and associations across population. © 2018 Elsevier B.V.

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Держатели документа:
Siberian Federal University, Svobodny pr. 79, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Akademgorodok 50/50, Krasnoyarsk, Russian Federation
Blokhin Cancer Research Center, Moscow, Russian Academy of Medical Sciences, Kashirskoye Shosse 24, Moscow, Russian Federation
Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk Lavrentiev Avenue 8, Novosibirsk, Russian Federation
State Medical University named after V.F. Voyno-Yasenetsky, Partizana Zheleznyaka St. 1, Krasnoyarsk, Russian Federation
Regional Clinical Oncology Center named after A.I. Kryzhanovsky, 1 Smolenskaya Str.16, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Bashmakova, E. E.; Krasitskaya, V. V.; Bondar, A. A.; Eremina, E. N.; Slepov, E. V.; Zukov, R. A.; Frank, L. A.

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11.

Bios-3: Siberian experiments in bioregenerative life support / F. B. Salisbury, J. I. Gitelson, G. M. Lisovsky // BioScience. - 1997. - Vol. 47, Is. 9. - P575-585 . - ISSN 0006-3568
Кл.слова (ненормированные):
agriculture -- Chlorella -- construction work and architectural phenomena -- crop -- energy metabolism -- evaluation -- growth, development and aging -- human -- metabolism -- methodology -- microbiology -- microclimate -- NASA Discipline Life Support Systems -- Non-NASA Center -- photon -- review -- Russian Federation -- space flight -- NASA Discipline Life Support Systems -- Non-NASA Center -- Agriculture -- Chlorella -- Crops, Agricultural -- Ecological Systems, Closed -- Energy Metabolism -- Environment, Controlled -- Environmental Microbiology -- Evaluation Studies -- Facility Design and Construction -- Humans -- Life Support Systems -- Photons -- Siberia -- Space Flight -- Space Simulation

Scopus
Держатели документа:
Dept. Plants, Soils, Biometeorology, College of Agriculture, Utah State University, Logan, UT 84322-4820, United States
Institute of Biophysics, Academy of Sciences of Russia, Siberian Branch, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Salisbury, F.B.; Gitelson, J.I.; Lisovsky, G.M.

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12.

Biotesting of Effluent and River Water by Lyophilized Luminous Bacteria Biotest / A. M. Kuznetsov, E. K. Rodicheva, S. E. Medvedeva // Field Analytical Chemistry and Technology. - 1998. - Vol. 2, Is. 5. - P267-275 . - ISSN 1086-900X
Кл.слова (ненормированные):
Bioluminescence -- Effluent -- Luminous bacteria biotest -- Toxicity -- Water quality
Аннотация: Waters of the Yenisei River, certain rivers and lakes of the Altai Territory, and effluents of some industrial factories in Krasnoyarsk were studied by luminous bacteria biotest Microbiosensor B17 677F. The lyophilized luminous bacteria Photobacterium phosphoreum from the IBSO collection were used to design this biotest. The bioluminescent test is based on bioluminescence quenching resulting from the action of water samples on luminous bacteria. The test results indicated locations and zones of impaired water quality. The heaviest pollution of water in the Yenisei River was recorded in the zones 0-116 km downstream from Krasnoyarsk (Krasnoyarsk and satellite towns). The effluents of most factories were found to be toxic. Underground and surface waters of some areas of the Altai Territory had different toxicity levels; there were deviations from the norm in most water samples taken from the different lakes and rivers. The data from this study show that the luminous bacteria biotest is simple and convenient, and that the results obtained are within acceptable levels of accuracy for the evaluation of the toxicity of effluent and river water. It takes no more than 30 min to do the biotest. It can be used in ecological monitoring like the Microtox toxicity test. В© 1998 John Wiley & Sons, Inc.

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Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kuznetsov, A.M.; Rodicheva, E.K.; Medvedeva, S.E.

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13.

Comparative evaluation of total peroxidase and catalase activities during light emission of luminous fungus Neonothopanus nambi / O. A. Mogilnaya, N. O. Ronzhin, V. S. Bondar // Mycosphere. - 2016. - Vol. 7, Is. 4. - P499-510, DOI 10.5943/mycosphere/7/4/9 . - ISSN 2077-7000
Кл.слова (ненормированные):
Basidiomycetes -- Hydrogen peroxide -- Luminescence -- Stress
Аннотация: Submerged cultivation of luminous fungus Neonothopanus nambi under orbital stirring causes formation of pellets with smooth or rough surfaces. The experiments showed that luminescence of the pellets washed in water increased considerably. Previous studies suggested possible participation of peroxidases in the light emitting reaction. In this study, oxidative azo coupling reaction accompanied by formation of chromogen was used to evaluate peroxidase activity in vivo, in brightly luminescent pellets and in pellets with low luminescence intensity (dim ones). Staining of the brightly luminescent pellets took a few minutes, and their staining intensity was several times higher than that of the dim pellets. From the results of in vivo experiments it was concluded that the bright pellets differed from the dim ones in the production of hydrogen peroxide, or, possibly, other peroxides. Measurements of total peroxidase and catalase activities in pellet extracts also showed an increase in enzyme activities along with an increase in luminescence intensity of native pellets. However, results of the in vitro experiments do not definitively suggest a direct relationship between luminescence and activity of these enzymes. We assume that luminescence of this fungal species may be an additional way to neutralize peroxide compounds under stress. © Guizhou Academy of Agricultural Sciences.

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Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Mogilnaya, O. A.; Ronzhin, N. O.; Bondar, V. S.

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14.

CORRELATION APPROACH TO COMPARING NUCLEOTIDE-SEQUENCES [Текст] / A. N. GORBAN, T. G. POPOVA, M. G. SADOVSKY // Zhurnal Obshchei Biol. - 1994. - Vol. 55, Is. 04.05.2013. - P. 420-430. - Cited References: 22 . - ISSN 0044-4596

РУБ Biology

Аннотация: A new method of evaluation of similarity between two nucleotide sequences is proposed. It is based on comparing frequency/correlation dictionaries of the sequences under investigation. The dictionary is a set of all strings of various length occurring within the sequences accompanied by their frequencies. The method proposed allows to compare sequences of arbitrary lengths, its advantage is absence of necessity of informal (expert) choice of the best fit. Efficiency of the method is demonstrated by comparison of several human genes and viruses.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
GORBAN, A.N.; POPOVA, T.G.; SADOVSKY, M.G.

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15.

Detoxification of AM-241 solutions by humic substances: Bioluminescent monitoring / T. Rozhko [et al.] // Analytical and Bioanalytical Chemistry. - 2011. - Vol. 400, Is. 2. - P329-334, DOI 10.1007/s00216-010-4442-9 . - ISSN 1618-2642
Кл.слова (ненормированные):
Detoxification -- Humic substances -- Ionizing radiation -- Luminous bacteria -- Bacterial cells -- Bottom sediments -- Humic substances -- Luminescent intensity -- Luminous bacteria -- Natural transformations -- Organic substances -- Photobacterium phosphoreum -- Physiological activity -- Protecting agent -- Water solutions -- Anoxic sediments -- Bacteriology -- Bioluminescence -- Detoxification -- Ionizing radiation -- Luminance -- Radiation shielding -- Radioactivity -- Bacteria -- americium -- radioisotope -- article -- bioremediation -- chemistry -- environmental monitoring -- evaluation -- humic substance -- instrumentation -- luminescence -- metabolism -- methodology -- Photobacterium -- water pollutant -- Americium -- Biodegradation, Environmental -- Environmental Monitoring -- Humic Substances -- Luminescence -- Photobacterium -- Radioisotopes -- Water Pollutants, Chemical -- Bacteria (microorganisms) -- Photobacterium phosphoreum
Аннотация: The study addresses the effect of humic substances on marine luminous bacteria Photobacterium phosphoreum exposed to Am-241 (3,000 Bq L -1, water solution). Luminescent intensity of the bacteria was applied as a marker of their physiological activity. Humic substances have been found to reduce the effect of Am-241 on luminescence, decrease damage to cells, and change distribution of Am-241 between bacterial cells and intercellular media. It was shown that water-soluble humic substances, being products of natural transformation of organic substances in soil and bottom sediments, can serve as protecting agents for water microorganisms exposed to alpha radionuclides. В© 2010 Springer-Verlag.

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Держатели документа:
Siberian Federal University, Krasnoyarsk 660041, Russian Federation
Institute of Biophysics SB RAS, Krasnoyarsk 660036, Russian Federation
Irkutsk State University, Irkutsk 664003, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Rozhko, T.; Bondareva, L.; Mogilnaya, O.; Vydryakova, G.; Bolsunovsky, A.; Stom, D.; Kudryasheva, N.

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16.

Development and characterization of ceftriaxone-loaded P3HB-based microparticles for drug delivery / A. V. Murueva [et al.] // Dry. Technol. - 2018, DOI 10.1080/07373937.2018.1487451 . - Article in press. - ISSN 0737-3937
Кл.слова (ненормированные):
Ceftriaxone -- drug release -- emulsification technique -- microbiological evaluation -- P3HB-microparticles -- spray-drying -- Drying -- Emulsification -- Encapsulation -- Functional polymers -- Spray drying -- Targeted drug delivery -- Anti-bacterial activity -- Ceftriaxone -- Double emulsifications -- Drug release -- Encapsulation efficiency -- Micro-particles -- Microbiological evaluation -- Polymeric microparticles -- Controlled drug delivery
Аннотация: In this study, polymer-based microparticles are used to improve the therapeutic properties of ceftriaxone (CEF) and render them safer. Poly-3-hydroxybutyrate (P3HB) and poly-3-hydroxybutyrate/polyethylene glycol (P3HB-PEG)-based microparticles were prepared by two methods: a double emulsification technique and spray-drying. The microparticles were characterized in terms of size and zeta potential, morphology, total drug loading and drug release. The microparticles had spherical shapes with diameters of a size range from 0.74 to 1.55 µm (emulsification technique) and from 3.84 to 6.51 µm (spray-drying); CEF encapsulation efficiency was around 63% and 49% for these methods respectively. The CEF release from microparticles obtained by spray-drying reached 100% after 150 h, while for microparticles obtained by emulsification technique the total release of CEF did not exceed 34% after 312 h. The release profiles could be best explained by Zero order kinetics model, Higuchi and Korsmeyer-Peppas models, as the plots showed high linearity. Antibacterial activity of the microparticles was evaluated against gram-positive and gram-negative bacterial strains. In general, CEF encapsulation in polymeric microparticles preserves the therapeutic efficacy of the CEF and provides its prolonged effect. © 2018, © 2018 Taylor & Francis.

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Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Murueva, A. V.; Shershneva, A. M.; Abanina, K. V.; Prudnikova, S. V.; Shishatskaya, E. I.

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17.

Effect of low-dose ionizing radiation on luminous marine bacteria: radiation hormesis and toxicity [Text] / N. S. Kudryasheva, T. V. Rozhko // J. Environ. Radioact. - 2015. - Vol. 142. - P68-77, DOI 10.1016/j.jenvrad.2015.01.012. - Cited References:131. - This work was supported by the Russian Foundation for Basic Research, Grant No.13-04-01305a, the Program "Molecular and Cellular Biology" of the Russian Academy of Sciences, project VI 57.1.1. The part of the work (review of effects of americium-241) was supported by the Russian Science Foundation, Grant No. 14-14-00076. . - ISSN 0265-931X. - ISSN 1879-1700

РУБ Environmental Sciences
Рубрики:
RECOMBINANT LUMINESCENT MICROORGANISMS
PHOTOBACTERIUM-LEIOGNATHI
Кл.слова (ненормированные):
Marine bacteria -- Low-dose effects -- Radiation hormesis -- Radiotoxicity -- Reactive oxygen species
Аннотация: The paper summarizes studies of effects of alpha- and beta-emitting radionuclides (americium-241, uranium-235+238, and tritium) on marine microorganisms under conditions of chronic low-dose irradiation in aqueous media. Luminous marine bacteria were chosen as an example of these microorganisms; bioluminescent intensity was used as a tested physiological parameter. Non-linear dose-effect dependence was demonstrated. Three successive stages in the bioluminescent response to americium-241 and tritium were found: 1 - absence of effects (stress recognition), 2 - activation (adaptive response), and 3 - inhibition (suppression of physiological function, i.e. radiation toxicity). The effects were attributed to radiation hormesis phenomenon. Biological role of reactive oxygen species, secondary products of the radioactive decay, is discussed. The study suggests an approach to evaluation of non-toxic and toxic stages under conditions of chronic radioactive exposure. (C) 2015 Elsevier Ltd. All rights reserved.

WOS
Держатели документа:
Inst Biophys SB RAS, Krasnoyarsk 660036, Russia
Siberian Fed Univ, Krasnoyarsk 660041, Russia
Krasnoyarsk State Med Acad, Krasnoyarsk 660022, Russia

Доп.точки доступа:
Kudryasheva, N. S.; Rozhko, T. V.; Russian Foundation for Basic Research [13-04-01305a]; Program "Molecular and Cellular Biology" of the Russian Academy of Sciences [VI 57.1.1]; Russian Science Foundation [14-14-00076]

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18.

Evaluation of antitumor activity of rubomycin deposited in absorbable polymeric microparticles / E. I. Shishatskaya [et al.] // Bulletin of Experimental Biology and Medicine. - 2008. - Vol. 145, Is. 3. - P358-361, DOI 10.1007/s10517-008-0091-9 . - ISSN 0007-4888
Кл.слова (ненормированные):
Absorbable polymers -- Ehrlich's ascitic carcinoma -- Microencapsulation -- Polyhydroxybutyrate -- Rubomycin -- poly(3 hydroxybutyric acid) -- polymer -- rubomycin -- animal cell -- animal experiment -- animal model -- antineoplastic activity -- article -- Bagg albino mouse -- cancer inhibition -- cancer mortality -- cancer survival -- controlled study -- drug delivery system -- Ehrlich ascites tumor -- microencapsulation -- mouse -- nonhuman -- polymerization -- survival rate -- tumor cell -- tumor volume -- Absorption -- Animals -- Antibiotics, Antineoplastic -- Carcinoma, Ehrlich Tumor -- Daunorubicin -- Hydroxybutyrates -- Mice -- Mice, Inbred BALB C -- Microspheres -- Polyesters -- Mus
Аннотация: An experimental dosage form of rubomycin is developed: the drug is incorporated in absorbable polymeric (polyhydroxybutyrate) matrix in the form of microparticles. Antitumor efficiency of this rubomycin dosage form was studied in laboratory mice with transplanted Ehrlich ascitic carcinoma. Rubomycin deposited in polymeric microparticles exhibited pronounced antitumor activity, inhibited the proliferative activity of Ehrlich ascitic carcinoma, and improved survival of mice with tumors. This dosage form of the drug can be used for local injections. В© Springer Science+Business Media, Inc. 2008.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
International Center for Studies of Critical Conditions, Presidium of Krasnoyarsk Research Center of Siberian Division of Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Goreva, A.V.; Voinova, O.N.; Inzhevatkin, E.V.; Khlebopros, R.G.; Volova, T.G.

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19.

Evaluation of cytogenetic abnormalities in cells of submerged plant Elodea canadensis in the Yenisei River section affected by industrial pollution: Field studies and laboratory experiments / A. Y. Bolsunovsky [et al.] // Dokl. Biol. Sci. - 2015. - Vol. 460, Is. 1. - P27-31, DOI 10.1134/S0012496615010044 . - ISSN 0012-4966
Кл.слова (ненормированные):
Elodea canadensis

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of SciencesKrasnoyarsk, Russian Federation
ИБФ СО РАН

Доп.точки доступа:
Bolsunovsky, A.Y.; Zotina, T.A.; Medvedeva, M.Y.; Trofimova, E.A.

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20.

EVALUATION OF MAXIMAL VALUES FOR THE FACTORS OF LIGHT-SCATTERING EFFICIENCY IN THE WKB APPROXIMATION [Текст] / V. N. LOPATIN, K. A. SHAPOVALOV // Opt. Spektrosk. - 1991. - Vol. 71, Is. 3. - P. 494-497. - Cited References: 5 . - ISSN 0030-4034

РУБ Optics + Spectroscopy

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
LOPATIN, V.N.; SHAPOVALOV, K.A.

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