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Вид документа : Статья из журнала
Шифр издания : 577.34/И 73
Автор(ы) : Болсуновский, Александр Яковлевич, Трофимова, Елена Александровна, Дементьев, Дмитрий Владимирович, Карпов, Антон Дмитриевич
Заглавие : Интенсивность накопления урана-238 представителями разных экологических уровней экосистемы р. Енисей
Коллективы : Институт биофизики Сибирского отделения Российской Академии наук (Красноярск)
Место публикации : Вестник Томского государственного университета. Биология. - Томск: Национальный исследовательский Томский государственный университет, 2016. - № 2 (34) . - С. 161-171. - DOI 10.17223/19988591/34/11
ISSN: 1998-8591
УДК : 577.34 + 597.5
Ключевые слова (''Своб.индексиров.''): коэффициент накопления--трофический уровень--philolimnogammarus viridis--fointinalis antipyretica--thymallus arcticus--esox lucius)
Аннотация: В результате многолетних исследований (2010-2013) получены данные по накоплению урана-238 в гидробионтах разных трофических уровней экосистемы р. Енисей. Пробы водного мха (Fointinalis antipyretica), зообентоса (Philolimnogammarus viridis и Ph. Cyaneus), сибирского хариуса (Thymallus arcticus) и щуки (Esox lucius) отбирали в районе ранее зарегистрированного повышенного содержания урана в воде р. Енисей (вблизи радиоактивных сбросов Горно-химического комбината Росатома). Показано, что уран способен эффективно накапливаться из воды в биомассе всех исследованных гидробионтов. Максимальные коэффициенты накопления (КН) урана получены для водного мха, что многократно превышает КН урана для зообентоса и мышечной ткани рыб. Средние значения КН урана для зообентоса статистически значимо превышают КН в мышцах рыб за весь период наблюдения. Не выявлено статистически значимых отличий в накоплении урана рыбами разного трофического уровня (хариус и щука)The aim of the research was a comparative evaluation of the intensity of uranium-238 accumulation in the biomass of aquatic organisms, occupying different trophic levels in the ecosystem of the Yenisei river. Previously, uranium-238 at concentrations higher than the background ones was detected in water and sediment samples of the Yenisei River collected close to the discharge site of one of the Rosatom facilities (the Mining-and-Chemical Combine). However, radioecological studies of the Yenisei River ecosystem have not focused on uranium isotopes in aquatic organisms so far. The toxic effects of uranium on living organisms are known to be based on its both radioactive and chemical properties. We conducted a study on accumulation of uranium-238 in aquatic organisms at different trophic levels in the Yenisei River ecosystem between 2010 and 2013. We collected samples of aquatic moss (Fointinalis antipyretica), zoobenthos (Philolimnogammarus viridis and Ph. cyaneus), arctic grayling (Thymallus arcticus), and pike (Esox lucius) from the Yenisei region at a distance of 85-90 km downstream of Krasnoyarsk (5-10 km downstream of the radioactive discharge of the Mining-and-Chemical Combine). Data on samples are shown in Table 1. We determined uranium concentration in the samples of aquatic organisms by neutron activation analysis, in water - by ICP-MS (Table 2). The study shows that uranium can be effectively concentrated from water and accumulate in the biomass of all aquatic organisms used in experiments. The highest uranium concentration factors (CFs) were obtained for aquatic moss, and they were several times higher than the uranium CFs for zoobenthos and fish muscles. Based on the uranium concentration from water, all aquatic organisms of the Yenisei River used in this study can be ranked as follows: aquatic moss gammarids grayling = pike. No statistically significant difference has been found between uranium accumulation by fish of different trophic levels (Arctic grayling and pike). The uranium CFs obtained in this study is comparable with the literature data on the CFs of artificial radionuclides for aquatic moss and fish of the Yenisei River
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Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Kukoba N., Ivanova Y., Botvich I.
Заглавие : The influence of altitudinal zonality on spectral characteristics (MODIS/Terra) mountain forests of Western Sayan
Место публикации : Climate Change Impacts on High-Altitude Ecosystems . - 2015. - С. 637-643. - ISBN 9783319128597 (ISBN); 9783319128580 (ISBN), DOI 10.1007/978-3-319-12859-7_26
Ключевые слова (''Своб.индексиров.''): mountain forests of siberia--net primary production (npp)--vegetation indices
Аннотация: The purpose of this study is to find a relationship between changes in spectral characteristics (MODIS/Terra) of mountain forests and the altitude at which they grow. In the study area, which is located in the West Sayan Mountains (in South Siberia), the types of forest ecosystems change markedly with altitude. The study uses the data of the MODIS-NPP model intended for the evaluation of global net production. Results of the study show that the best approach to dividing mountain forests into different types is to use 8-day composites of satellite data collected at the beginning of the growing season (April-May). This is the time when the most significant differences are recorded between vegetation indices, including Normalized Difference Vegetation Index (NDVI), LAI, and EVI of the mountain forests growing in different altitudinal zones. © Springer International Publishing Switzerland 2015.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Krasitskaya, Vasilisa V., Goncharova, Natalia S., Biriukov, Vladislav V., Bashmakova, Eugenia E., Kabilov, Marsel R., Baykov, Ivan K., Sokolov, Aleksey E., Frank, Ludmila A.
Заглавие : The Ca2+-Regulated Photoprotein Obelin as a Tool for SELEX Monitoring and DNA Aptamer Affinity Evaluation
Колич.характеристики :6 с
Коллективы : Russian Foundation for Basic Research (RFBR)Russian Foundation for Basic Research (RFBR) [18-38-00531]
Место публикации : Photochem. Photobiol.: WILEY, 2020. - Article in press. - ISSN 0031-8655, DOI 10.1111/php.13274. - ISSN 1751-1097(eISSN)
Примечания : Cited References:25. - This work has been supported by the Russian Foundation for Basic Research (RFBR) under the grant no 18-38-00531.
Предметные рубрики: CARDIAC TROPONIN-I
BIOLUMINESCENCE
IMMUNOASSAY
APTASENSOR
DIAGNOSIS
Аннотация: Bioluminescent solid-phase analysis was proposed to monitor the selection process and to determine binding characteristics of the aptamer-target complexes during design and development of the specific aptamers. The assay involves Ca2+-regulated photoprotein obelin as a simple, sensitive and fast reporter. Applicability and the prospects of the approach were exemplified by identification of DNA aptamers to cardiac troponin I, a highly specific early biomarker for acute myocardial infarction. Two structurally different aptamers specific to various epitopes of troponin I were obtained and then tested in a model bioluminescent assay.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gitelson I.I., Degermendzhy A.G., Rodicheva E.K.
Заглавие : Self-restoration as fundamental property of CES providing their sustainability
Место публикации : Advances in Space Research. - 2003. - Vol. 31, Is. 7. - С. 1641-1648. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(03)00111-X
Ключевые слова (''Своб.индексиров.''): biocatalysts--ecosystems--genes--life support systems (spacecraft)--radiation damage--cell populations--space flight--space shuttle--article--biological model--biomass--cell division--chlorella--cytology--growth, development and aging--microclimate--radiation exposure--radiation response--ultraviolet radiation--biomass--cell division--chlorella--dose-response relationship, radiation--ecological systems, closed--life support systems--models, biological--ultraviolet rays
Аннотация: Sustainability is one of the most important criteria and evaluation of human life support systems intended for use during long space flights. The common feature of biological and physicochemical life support systems is that basically they are both catalytic. But there are two fundamental properties distinguishing biological systems: 1) they are auto-catalytic: their catalysts - enzymes of protein nature - are continuously reproduced when the system functions; 2) the program of every process performed by enzymes and the program of their reproduction are inherent in the biological system itself - in the totality of genomes of the species involved in the functioning of the ecosystem. Actually, one cell with the genome capable of the phenotypic realization is enough for the self-restoration of the function performed by the cells of this species in the ecosystem. The continuous microalgal culture of Chlorella vulgaris was taken to investigate quantitatively the process of self-restoration in unicellular algae population. Based on the data obtained, we proposed a mathematical model of the restoration process in a cell population that has suffered an acute radiation damage. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Gitelson I.I., Degermendzhy A.G., Rodicheva E.K.
Заглавие : Self-restoration as fundamental property of CES providing their sustainability
Колич.характеристики :8 с
Место публикации : SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - P1641-1648. - ISBN 0273-1177, DOI 10.1016/S0273-1177(03)00111-X
Примечания : Cited References: 8
Предметные рубрики: LIFE-SUPPORT
Аннотация: Sustainability is one of the most important criteria in the creation and evaluation of human life support systems intended for use during long space flights. The common feature of biological and physicochemical life support systems is that basically they are both catalytic. But there are two fundamental properties distinguishing biological systems: 1) they are auto-catalytic: their catalysts - enzymes of protein nature - are continuously reproduced when the system functions; 2) the program of every process performed by enzymes and the program of their reproduction are inherent in the biological system itself - in the totality of genomes of the species involved in the functioning of the ecosystem. Actually, one cell with the genome capable of the phenotypic realization is enough for the self-restoration of the function performed by the cells of this species in the ecosystem. The continuous microalgal culture of Chlorella vulgaris was taken to investigate quantitatively the process of self-restoration in unicellular algae population. Based on the data obtained, we proposed a mathematical model of the restoration process in a cell population that has suffered an acute radiation damage. (C) 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petrova A. S., Lukonina A. A., Dementyev D. V., Bolsunovsky A. Ya. , Popov A. V., Kudryasheva N. S.
Заглавие : Protein-based fluorescent bioassay for low-dose gamma radiation exposures
Место публикации : Anal. Bioanal. Chem.: Springer Verlag, 2018. - ISSN 16182642 (ISSN) , DOI 10.1007/s00216-018-1282-5
Ключевые слова (''Своб.индексиров.''): bioassay--enzymes--fluorescence/luminescence--fluorescent protein--gamma radiation--radiotoxicity--efficiency--enzymes--fluorescence--gamma rays--proteins--proton transfer--fluorescence characteristics--fluorescence intensities--fluorescence spectra--fluorescence/luminescence--fluorescent protein--photochemical process--physiological liquids--radiotoxicity--bioassay
Аннотация: The study suggests an application of a coelenteramide-containing fluorescent protein (CLM-CFP) as a simplest bioassay for gamma radiation exposures. “Discharged obelin,” a product of the bioluminescence reaction of the marine coelenterate Obelia longissima, was used as a representative of the CLM-CFP group. The bioassay is based on a simple enzymatic reaction—photochemical proton transfer in the coelenteramide-apoprotein complex. Components of this reaction differ in fluorescence color, providing, by this, an evaluation of the proton transfer efficiency in the photochemical process. This efficiency depends on the microenvironment of the coelenteramide within the protein complex, and, hence, can evaluate a destructive ability of gamma radiation. The CLM-CFP samples were exposed to gamma radiation (137Cs, 2 mGy/h) for 7 and 16 days at 20 °C and 5 °C, respectively. As a result, two fluorescence characteristics (overall fluorescence intensity and contributions of color components to the fluorescence spectra) were identified as bioassay parameters. Both parameters demonstrated high sensitivity of the CLM-CFP-based bioassay to the low-dose gamma radiation exposure (up to 100 mGy). Higher temperature (20 °C) enhanced the response of CLM-CFP to gamma radiation. This new bioassay can provide fluorescent multicolor assessment of protein destruction in cells and physiological liquids under exposure to low doses of gamma radiation. [Figure not available: see fulltext.]. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
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Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Bartsev S.I., Okhonin V.A.
Заглавие : Potentialities of theoretical and experimental prediction of life support systems reliability
Колич.характеристики :6 с
Место публикации : LIFE SCIENCES: ARTIFICIAL ECOSYSTEMS. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON PRESS LTD, 1999. - Vol. 24: F4 3 and F4 4 Symposia of COSPAR Scientific Commission F Held at 31st COSPAR Scientific Assembly (JUL 14-21, 1996, BIRMINGHAM, ENGLAND), Is. 3. - P407-412. - ISBN 0273-1177, DOI 10.1016/S0273-1177(99)00491-3
Примечания : Cited References: 13
Аннотация: To develop and design Life Support Systems it is necessary to evaluate their reliability. However direct experiments take much time, are very expensive, and therefore are practically impossible. Promising way is to use approximate estimates of reliability, which need essentially fewer amounts of experimental data. Two types of estimates of Life Support System reliability - additive and multiplicative ones are considered in the paper. Additive estimate is based on the assumption that total system failure probability is low and therefore it can be considered as the sum of failure probability of separate units. Additive approach allows obtaining near lower-bounded estimate of failure probability. Multiplicative estimate allows evaluating the possibility of system catastrophe due to simultaneous effect of several factors when each of them separately is not dangerous. Evaluation shows that the possible error of reliability forecast increases with the increasing of number of external factors faster than exponential function. An illustration of the ecological similarity approach as promising tool for providing estimation of full-scale system reliability by means the set of small similar experimental models. (C) 1999 COSPAR. Published by Elsevier Science Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shershneva, Anna, Murueva, Anastasiya, Nikolaeva, Elena, Shishatskaya, Ekaterina, Volova, Tatiana
Заглавие : Novel spray-dried PHA microparticles for antitumor drug release
Колич.характеристики :12 с
Коллективы : Russian Academy of Sciences [AAAA-A17- 117013050028-8]
Место публикации : Dry. Technol.: TAYLOR & FRANCIS INC, 2018. - Vol. 36, Is. 11. - С. 1387-1398. - ISSN 0737-3937, DOI 10.1080/07373937.2017.1407940. - ISSN 1532-2300(eISSN)
Примечания : Cited References:67. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences: [Project no. AAAA-A17- 117013050028-8].
Предметные рубрики: DRYING METHOD
IN-VITRO
BIODEGRADABLE NANOPARTICLES
ORAL
Ключевые слова (''Своб.индексиров.''): 5-fluorouracil--efficiency--microparticles--paclitaxel--polyhydroxyalkanoates--spray-drying
Аннотация: The production of poly-3-hydroxybutyrate (P3HB) and poly-3-hydroxybutyrate/polyethylene glycol (PEG)-based microparticles, loaded with antitumor drugs paclitaxel (PTX) and 5-Fluorouracil (5-FU) by spray-drying technique, was investigated. The average diameter of microparticles was found to be 3.4 +/- 0.5 pm and zeta potential was about -44 mV. The addition of surfactant PEG did not show any effect on the morphological characteristics of the particles. But the chemical structure of drug influenced on the properties. Microparticles had heterogeneous pores on the surface when the hydrophobic PTX was encapsulated. It was established that the addition of surfactant positively influenced on the properties of particles and led to the loading of 5-FU directly into the matrix. This is confirmed by the results of electron microscopy and dynamics of drug release in vitro. As a whole, the release profiles of PTX and 5-FU from composite P3HB/PEG microparticles were less than from P3HB microparticles. The results of the morphological evaluation of Hela cells demonstrated that the use of cytostatic drugs loaded in P3HB microparticles induces morphological changes associated with apoptosis (chromatin condensation, core fragmentation, margination of nucleus). Thus, the obtained results can serve as the basis for the development of new antitumor drugs of prolonged action, intended for various modes of administration.
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Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Ivanova Y., Soukhovolsky V.
Заглавие : Net primary production in forest ecosystem of middle siberia: Assessment using a model of tree component phytomass distribution
Место публикации : Climate Change Impacts on High-Altitude Ecosystems . - 2015. - С. 627-635. - ISBN 9783319128597 (ISBN); 9783319128580 (ISBN), DOI 10.1007/978-3-319-12859-7_25
Ключевые слова (''Своб.индексиров.''): forest stands of middle siberia--net primary production (npp)--root phytomass
Аннотация: The authors propose several methods of net primary production (NPP) evaluation for tree stands of Middle Siberia. The authors’ approach is based on the model of the distribution of tree (tree stand) phytomass among different tree components (stem, roots, branches, foliage) using the Zipf-Pareto equation. This model can be used not only to calculate the phytomass and NPP of the tree aboveground parts but also to determine quite accurately the phytomass and NPP of the roots. Several approaches to calculation of tree stand NPP have been proposed, depending on the amount of available data. © Springer International Publishing Switzerland 2015.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Grodnitskaya I. D., Sorokin N. D., Evgrafova S. Y., Antonov G. I., Syrtsov S. N., Aleksandrov D. E., Trusova M. Y., Koroban N. V.
Заглавие : Microbial transformation of carbon CH4 and CO2 in permafrost-affected soils in tundra and forest ecosystems in Siberia
Место публикации : Russ. J. For. Sci.: Izdatel'stvo Nauka, 2017. - Is. 2. - С. 111-127. - ISSN 00241148 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacterial diversity--ch4 and co2 emission--dynamics and activity of microbial complexes--frost-crack polygons--larch forests--tundra--сryogenic soils
Аннотация: We studied structure, dynamics and functional (biogeochemical) activity of microbial complexes of cryogenic soils in larch forests in Central Evenkia and polygonal tundra on Samoilovskii Island, Lena Delta. We found that daily flux of methane from soil surface is 3–5 times less in forest soil than in the center of polygon in tundra. Short-term heating to 18.5–22.5 °C of permafrost-affected soil in larch forest caused sweetening of soil solution, shrinkage of eco-trophic groups of microorganisms and microbial biomass, as well as increase in greenhouse gases (CO2 and CH4) emission to the air. Notably the permafrost-affected soil on sandy deposits in tundra had highest microbial diversity of methanogenic archaea including Methanobacteriaceae, Methanomicrobiaceae, Methanosarcinaceae, Methanosaetaceae families. On the other hand only Methanosarcinacea were found in cryosols of larch forest. Both type I and type II methanotrophs were found in the forest soil, while only type II methanotrophs occurred in tundra soil. © 2017, Izdatel’stvo Nauka. All rights reserved.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev, Michail I., Sushchik, Nadezhda N., Tolomeev, Alexander P., Dgebuadze, Yury Yu
Заглавие : Meta-analysis of factors associated with omega-3 fatty acid contents of wild fish
Колич.характеристики :23 с
Коллективы : Russian Science Foundation [16-14-10001]
Место публикации : Rev. Fish. Biol. Fish.: SPRINGER, 2018. - Vol. 28, Is. 2. - С. 277-299. - ISSN 0960-3166, DOI 10.1007/s11160-017-9511-0. - ISSN 1573-5184(eISSN)
Примечания : Cited References:138. - The work was supported by a Russian Science Foundation Grant (No. 16-14-10001).
Предметные рубрики: FATTY-ACID-COMPOSITION
DIETARY DOCOSAHEXAENOIC ACID
LONG-CHAIN
Ключевые слова (''Своб.индексиров.''): docosahexaenoic acid--ecomorphological factors--eicosapentaenoic acid--nutritive value--phylogenetic factors
Аннотация: Fish are recognized as the main source of physiologically important omega-3 long-chain polyunsaturated fatty acids, namely, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), for human nutrition. However, muscle tissue contents of these fatty acids in diverse fish species, i.e., their nutritive value for humans, varied within two orders of magnitude. We reviewed contents of EPA and DHA, measured by similar methods using an internal standard during chromatography as mg per g of wet mass in 172 fish species belonging to 16 orders, to evaluate probable variations in phylogenetic and ecological drivers. EPA + DHA content varied from 25.6 mg g(-1) of wet mass (Sardinops sagax) to 0.12 mg g(-1) (Gymnura spp.). Multidimensional redundancy analysis revealed that among phylogenetic, ecomorphological and abiotic environmental factors, the highest proportion of variation contribution belonged to the shared contribution of sets of phylogenetic and ecomorphological factors. Specifically, the highest values of EPA + DHA content were characteristic of fish belonging to the orders Clupeiformes or Salmoniformes, were pelagic fast swimmers, ate zooplankton and inhabited marine waters or migrated from fresh to marine waters (anadromous migrations). High EPA and DHA content in muscle tissues of the above species appeared to be a metabolic adaptation for fast continuous swimming. In contrast to common beliefs, our meta-analysis did not support the significant influence of higher trophic levels (piscivory) and cold environments (homeoviscous adaptation) on EPA and DHA content in fish. However, many causes of high and low levels of physiologically important fatty acids in certain fish species remained unexplained and require evaluation in future studies.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tikhomirov A.A., Ushakova S.A., Manukovsky N.S., Lisovsky G.M., Kudenko Yu.A., Kovalev V.S., Gubanov V.G., Barkhatov Yu.V., Gribovskaya I.V., Zolotukhin I.G., Gros J.B., Lasseur Ch.
Заглавие : Mass exchange in an experimental new-generation life support system model based on biological regeneration of environment
Место публикации : Advances in Space Research. - 2003. - Vol. 31, Is. 7. - С. 1711-1720. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(03)80017-0
Ключевые слова (''Своб.индексиров.''): biomass--photosynthesis--plants (botany)--transpiration--mass exchange--life support systems (spacecraft)--ammonia--nitrogen--oxygen--biosphere--animal--annelid worm--article--biological model--biomass--bioremediation--evaluation--growth, development and aging--human--metabolism--microclimate--photosynthesis--pleurotus--radish--wheat--ammonia--animals--biodegradation, environmental--biomass--ecological systems, closed--evaluation studies--humans--life support systems--models, biological--nitrogen--oligochaeta--oxygen--photosynthesis--pleurotus--raphanus--triticum
Аннотация: An experimental model of a biological life support system was used to evaluate qualitative and quantitative parameters of the internal mass exchange. The photosynthesizing unit included the higher plant component (wheat and radish), and the heterotrophic unit consisted of a soil-like substrate, California worms, mushrooms and microbial microflora. The gas mass exchange involved evolution of oxygen by the photosynthesizing component and its uptake by the heterotroph component along with the formation and maintaining of the SLS structure, growth of mushrooms and California worms, human respiration, and some other processes. Human presence in the system in the form of "virtual human" that at regular intervals took part in the respirative gas exchange during the experiment. Experimental data demonstrated good oxygen/carbon dioxide balance, and the closure of the cycles of these gases was almost complete. The water cycle was nearly 100% closed. The main components in the water mass exchange were transpiration water and the watering solution with mineral elements. Human consumption of the edible plant biomass (grains and roots) was simulated by processing these products by a unique physicochemical method of oxidizing them to inorganic mineral compounds, which were then returned into the system and fully assimilated by the plants. The oxidation was achieved by "wet combustion" of organic biomass, using hydrogen peroxide following a special procedure, which does not require high temperature and pressure. Hydrogen peroxide is produced from the water inside the system. The closure of the cycle was estimated for individual elements and compounds. Stoichiometric proportions are given for the main components included in the experimental model of the system. Approaches to the mathematical modeling of the cycling processes are discussed, using the data of the experimental model. Nitrogen, as a representative of biogenic elements, shows an almost 100% closure of the cycle inside the system. The proposed experimental model of a biological system is discussed as a candidate for potential application in the investigations aimed at creating ecosystems with largely closed cycles of the internal mass exchange. The formation and maintenance of sustainable cycling of vitally important chemical elements and compounds in biological life support systems (BLSS) is an extremely pressing problem. To attain the stable functioning of biological life support systems (BLSS) and to maintain a high degree of closure of material cycles in them, it is essential to understand the character of mass exchange processes and stoichiometric proportions of the initial and synthesized components of the system. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gitelson I.I., Terskov I.A., Kovrov B.G., Lisovskii G.M., Okladnikov Yu.N., Sid'ko F.Ya., Trubachev I.N., Shilenko M.P., Alekseev S.S., Pan'kova I.M., Tirranen L.S.
Заглавие : Long-term experiments on man's stay in biological life-support system
Место публикации : Advances in Space Research. - 1989. - Vol. 9, Is. 8. - С. 65-71. - ISSN 02731177 (ISSN)
Ключевые слова (''Своб.индексиров.''): agricultural products--ecosystems--food products--personnel - health--space flight - manned flight--bios-3 complex--phytotrons--spacecraft--water--air conditioning--article--biomass--crop--evaluation--growth, development and aging--human--instrumentation--light--metabolism--methodology--microclimate--photosynthesis--plant--waste management--wheat--air conditioning--biomass--crops, agricultural--ecological systems, closed--evaluation studies--humans--life support systems--light--photosynthesis--plants--triticum--waste management--water
Аннотация: We describe the experimental system having maximal possible closure of material recycling in an ecosystem, including people and plants, which was carried out in a hermetically sealed experimental complex "BIOS-3", 315 m2 in volume. The system included 2 experimentators and 3 phytotrons with plants (total sowing area of 63 m2). Plants were grown with round-the-clock lamp irradiation with 130 Wm-2 PAR intensity. The plants production was food for people. Water exchange of ecosystem, as well as gas exchange, was fully closed excluding liquids and gas samples taken for chemical analysis outside the system. The total closure of material turnover constituted 91%. Health state of the crew was estimated before, during and after the experiment. A 5-months period did not affect their health. The experiments carried out prove that the closed ecosystem of "man-plants" is a prototype of a life-support system for long-term space expeditions. В© 1989.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : PUKHOV K.I., YAKHNINA E.I., MAKARSKAYA G.V., PUKHOVA Y.I., SAVCHENKO V.G.
Заглавие : INVESTIGATION OF WHOLE-BLOOD LEUKOCYTE FUNCTIONAL-ACTIVITY WITH THE USE OF CHEMILUMINESCENCE DURING PLASMAPHERESIS
Колич.характеристики :4 с
Место публикации : Gematol. Transfuziol.: MINISTERSTVO ZDRAVOOKHRANENIYA, 1991. - Vol. 36, Is. 9. - P19-22. - ISSN 0234-5730
Примечания : Cited References: 0
Аннотация: Repeated plasmapheresis sessions attended by chemiluminescent evaluation of cellular immunity parameters lead to the recovery of the whole blood leucocyte activity in patients with unhealing gastroduodenal ulcers. The kinetic analysis of the cellular chemiluminescent response has shown that, among all the components of the plasmapheresis procedure, hemorrhage is the most important factor increasing the functional activity of phagocytes.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Adamovich V.V., Zotina T.A., Degermendzhy A.G.
Заглавие : Interaction coefficients in the Lake Shira algal-bacterial community
Место публикации : Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - С. 261-270. - ISSN 13862588 (ISSN) , DOI 10.1023/A:1015638420766
Ключевые слова (''Своб.индексиров.''): bacterioplankton--feedback coefficient--influence coefficient--phytoplankton--specific growth rate--bacterioplankton--interspecific interaction--microbial community--phytoplankton--quantitative analysis--saline lake--russian federation--algae--bacteria (microorganisms)
Аннотация: A new method is proposed to evaluate the interaction coefficients in microbial communities interacting due to physical-chemical environmental factors. This method differs from the classical one suggested by E.P.Odum. Redefinition of the interaction coefficients allows the evaluation of the experimental (actual) and theoretical values of the coefficients for the hypothetical interaction layout. The interaction layout is considered to be a set of factors, the values of their transformation ratios and the form of dependence of a population's specific growth rate on these factors. A comparison of theoretical and experimental values of interaction coefficients enables us to assess the adequacy of the hypothetical interaction scheme in the microbial communities. The aim of the work is to test the suggested method on a natural algal-bacterial community of Shira Lake (Khakasia, Russia), which has been the object of detailed and concerted limnological studies and for which detailed data are available. Feedback coefficients of phyto- and bacterioplankton and the coefficients of influence of phytoplankton on bacterioplankton have been defined. Dominance of negative experimental IC values has been experimentally shown, which is indicative of the negative feedback in bacterio- and phytoplankton links of Shira Lake and of negative interpopulation (phytoplankton on bacterioplankton) interactions. The considerable differences observed between the experimental and theoretical coefficients indicates inadequacy of the assumed interaction layout of the community under study. Further investigations are needed to provide a precise sketch of the interactions.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Gitelson I.I.
Заглавие : In vivo toxicological evaluation of polyhydroxyalkanoates.
Место публикации : Doklady Biological Sciences. - 2002. - Vol. 383, Is. 1-6. - С. 109-111. - ISSN 00124966 (ISSN)
Ключевые слова (''Своб.индексиров.''): alkane--animal--animal behavior--article--cytology--drug effect--erythrocyte sedimentation rate--female--heart--kidney--liver--lymphoid tissue--male--rat--spleen--wistar rat--alkanes--animals--behavior, animal--blood sedimentation--female--heart--kidney--liver--lymphoid tissue--male--rats--rats, wistar--spleen
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Rota, Emilia, Martinsson, Svante, Erseus, Christer, Petushkov, Valentin N., Rodionova, Natalja S., Omodeo, Pietro
Заглавие : Green light to an integrative view of Microscolex phosphoreus (Duges, 1837) (Annelida: Clitellata: Acanthodrilidae)
Колич.характеристики :15 с
Коллективы : Russian Foundation for Basic Research [15-04-02695-a]; Adlerbert Foundation; [01201351504]
Место публикации : Zootaxa: MAGNOLIA PRESS, 2018. - Vol. 4496, Is. 1. - С. 175-189. - ISSN 1175-5326, DOI 10.11646/zootaxa.4496.1.13. - ISSN 1175-5334(eISSN)
Примечания : Cited References:53. - This work was partially supported by Grant 15-04-02695-a from the Russian Foundation for Basic Research and the state budget allocated to the fundamental research at the Russian Academy of Sciences (project No 01201351504). Funding for travel to Western Australia (CE, SM) was provided by the Adlerbert Foundation.
Предметные рубрики: BIOLUMINESCENT EARTHWORMS
LUMINOUS EARTHWORM
OLIGOCHAETA
HAPLOTYPES
Ключевые слова (''Своб.индексиров.''): peregrine earthworm--cryptic diversity--morphology--dna-barcoding--bioluminescence
Аннотация: The small synanthropic and peregrine earthworm Microscolex phosphoreus (Duges, 1837) is reported for the first time from Siberia. Morphological and DNA barcode (COI) analyses of this and widely separate samples worldwide demonstrate that, as currently identified, M. phosphoreus is a heterogeneous taxon, with divergent lineages occurring often in the same locality and hardly providing geographically structured genetic signals. The combined morphological and genetic evidence suggests that at least four of the found clades should be reclassified as separate species, both morphologically and genetically distinct from each other. However, as the specimen number was limited and only the COI gene was studied for the genetic work, we hesitate in formally describing new species. There would also be the problem of assigning the available names to specific lineages. Our findings encourage careful external and anatomical examination and using reliable characters such as the interchaetal distances and spermathecal morphology for correct identification and for deeper evaluation of cryptic diversity in this interesting bioluminescent worm.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petrova, Alena S., Lukonina, Anna A., Badun, Gennadii A., Kudryasheva, Nadezhda S.
Заглавие : Fluorescent coelenteramide-containing protein as a color bioindicator for low-dose radiation effects
Колич.характеристики :5 с
Коллективы : Russian Academy of Sciences [01201351504]; Russian Foundation for Basic Research [16-34-00695]
Место публикации : Anal. Bioanal. Chem.: SPRINGER HEIDELBERG, 2017. - Vol. 409, Is. 18. - С. 4377-4381. - ISSN 1618-2642, DOI 10.1007/s00216-017-0404-9. - ISSN 1618-2650(eISSN)
Примечания : Cited References:22. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences (project 01201351504) and by the Russian Foundation for Basic Research, Grant No. 16-34-00695.
Предметные рубрики: LUMINOUS MARINE-BACTERIA
DISCHARGED-OBELIN
AEQUORIN
Ключевые слова (''Своб.индексиров.''): fluorescent protein--coelenteramide--discharged photoprotein obelin--multicolor bioindicator--radiotoxicity
Аннотация: The study addresses the application of fluorescent coelenteramide-containing proteins as color bioindicators for radiotoxicity evaluation. Biological effects of chronic low-dose radiation are under investigation. Tritiated water (200 MBq/L) was used as a model source of low-intensive ionizing radiation of beta type. 'Discharged obelin,' product of bioluminescent reaction of marine coelenterate Obelia longissimi, was used as a representative of the coelenteramide-containing proteins. Coelenteramide, fluorophore of discharged obelin, is a photochemically active molecule; it produces fluorescence forms of different color. Contributions of 'violet' and 'blue-green' forms to the visible fluorescence serve as tested parameters. The contributions depend on the coelenteramide's microenvironment in the protein, and, hence, evaluate distractive ability and toxicity of radiation. The protein samples were exposed to beta radiation for 18 days, and maximal dose accumulated by the samples was 0.28 Gy, being close to a tentative limit of a low-dose interval. Increase of relative contribution of 'violet' fluorescence under exposure to the beta irradiation was revealed. High sensitivity of the protein-based test system to low-dose ionizing radiation (to 0.03 Gy) was demonstrated. The study develops physicochemical understanding of radiotoxic effects.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mogilnaya O. A., Ronzhin N. O., Bondar V. S.
Заглавие : Extracellular peroxidase activity and light emission of the mycelium of the basidiomycete neonothopanus nambi in the presence of ?-glucosidase
Место публикации : Biophysics: Pleiades Publishing, 2018. - Vol. 63, Is. 1. - С. 93-99. - ISSN 00063509 (ISSN) , DOI 10.1134/S0006350918010104
Ключевые слова (''Своб.индексиров.''): basidiomycetes--cell wall--luminescence--peroxidase--?-glucosidase
Аннотация: A comparative evaluation of the level of extracellular peroxidase activity and light-emission intensity of the mycelium of the luminescent basidiomycete Neonothopanus nambi in the presence of ?-glucosidase was performed. The enzyme activity damages the hyphae of the fungus leading to osmotic imbalance, partial degradation of the mycelium, and release of extracellular peroxidases into the incubation medium. The presence of ?-glucosidase reduces the time necessary to reach the maximum luminescence. Putative biochemical mechanisms that underlie the stimulation of reactive oxygen species formation (first and foremost, of hydrogen peroxide) in the N. nambi mycelium in the presence of ?-glucosidase are proposed. © O.A. Mogilnaya, N.O. Ronzhin, V.S. Bondar and Pleiades Publishing, Inc., 2018.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kudryasheva N. S., Petrova A. S., Dementyev D. V., Bondar A. A.
Заглавие : Exposure of luminous marine bacteria to low-dose gamma-radiation
Место публикации : J. Environ. Radioact.: Elsevier Ltd, 2017. - Vol. 169-170. - С. 64-69. - ISSN 0265931X (ISSN) , DOI 10.1016/j.jenvrad.2017.01.002
Ключевые слова (''Своб.индексиров.''): bioassay--low-dose gamma-radiation--luminous marine bacteria--mutagenic effect--radiotoxicity--temperature dependence--bacteria--bioassay--bioluminescence--gamma rays--ionizing radiation--irradiation--phosphorescence--physiological models--radiation effects--temperature distribution--low dose--marine bacterium--mutagenic effect--radiotoxicity--temperature dependence--radiation--bacteria (microorganisms)--photobacterium phosphoreum
Аннотация: The study addresses biological effects of low-dose gamma-radiation. Radioactive 137Cs-containing particles were used as model sources of gamma-radiation. Luminous marine bacterium Photobacterium phosphoreum was used as a bioassay with the bioluminescent intensity as the physiological parameter tested. To investigate the sensitivity of the bacteria to the low-dose gamma-radiation exposure (?250 mGy), the irradiation conditions were varied as follows: bioluminescence intensity was measured at 5, 10, and 20°С for 175, 100, and 47 h, respectively, at different dose rates (up to 4100 ?Gy/h). There was no noticeable effect of gamma-radiation at 5 and 10°С, while the 20°С exposure revealed authentic bioluminescence inhibition. The 20°С results of gamma-radiation exposure were compared to those for low-dose alpha- and beta-radiation exposures studied previously under comparable experimental conditions. In contrast to ionizing radiation of alpha and beta types, gamma-emission did not initiate bacterial bioluminescence activation (adaptive response). As with alpha- and beta-radiation, gamma-emission did not demonstrate monotonic dose-effect dependencies; the bioluminescence inhibition efficiency was found to be related to the exposure time, while no dose rate dependence was found. The sequence analysis of 16S ribosomal RNA gene did not reveal a mutagenic effect of low-dose gamma radiation. The exposure time that caused 50% bioluminescence inhibition was suggested as a test parameter for radiotoxicity evaluation under conditions of chronic low-dose gamma irradiation. © 2017 Elsevier Ltd
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