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Вид документа : Статья из сборника (выпуск продолж. издания)
Шифр издания :
Автор(ы) : Kiselev E. G., Zhila N. O., Volova T. G.
Заглавие : Biodegradable polymers - Perspectives and applications in agriculture
Место публикации : IOP Conference Series: Earth and Environmental Science: IOP Publishing Ltd, 2021. - Vol. 689: 2020 International Conference on Germany and Russia: Ecosystems Without Borders, EcoSystConfKlgtu 2020 (5 October 2020 through 10 October 2020, ) Conference code: 167944, Is. 1. - Ст.012036. - , DOI 10.1088/1755-1315/689/1/012036
Аннотация: The paper presents a brief overview of the results of the implementation of the project "Agropreparations of the new generation: a strategy of construction and realization". The first part contains the analysis of the growth of the wild-type strain Cupriavidus necator B-10646 (formerly eutrophus) and the synthesis of polyhydroxyalkanoates by this strain on various substrates: glycerol, palm oil, Siberian oil seed, sunflower seed oils, and oleic acid. On refined glycerin, a highly productive process is implemented when scaling up, allowing to obtain 128 ± 11 g / L PHA. Evaluation of oils has shown that palm oil is the best carbon substrate. The second part presents the results of the development of environmentally friendly slow-release pesticide formulations. They are a degradable matrix of poly-3-hydroxybutyrate mixed with natural materials (peat, clay, wood flour), into which a pesticide (metribuzin, tribenuron-methyl, fenoxaprop-P-ethyl, azoxystrobin, epoxiconazole, and tebuconazole) has been. The developed preparations showed high activity against pathogenic fungi and weeds and had a much weaker negative effect on the soil microflora. Studies of the degradation of the developed preparations and the release of pesticides into the soil confirm their effectiveness over a long period of time, up to 90 days. © Published under licence by IOP Publishing Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Krasitskaya, Vasilisa V., Goncharova, Natalia S., Biriukov, Vladislav V., Bashmakova, Eugenia E., Kabilov, Marsel R., Baykov, Ivan K., Sokolov, Aleksey E., Frank, Ludmila A.
Заглавие : The Ca2+-Regulated Photoprotein Obelin as a Tool for SELEX Monitoring and DNA Aptamer Affinity Evaluation
Колич.характеристики :6 с
Коллективы : Russian Foundation for Basic Research (RFBR)Russian Foundation for Basic Research (RFBR) [18-38-00531]
Место публикации : Photochem. Photobiol.: WILEY, 2020. - Article in press. - ISSN 0031-8655, DOI 10.1111/php.13274. - ISSN 1751-1097(eISSN)
Примечания : Cited References:25. - This work has been supported by the Russian Foundation for Basic Research (RFBR) under the grant no 18-38-00531.
Предметные рубрики: CARDIAC TROPONIN-I
BIOLUMINESCENCE
IMMUNOASSAY
APTASENSOR
DIAGNOSIS
Аннотация: Bioluminescent solid-phase analysis was proposed to monitor the selection process and to determine binding characteristics of the aptamer-target complexes during design and development of the specific aptamers. The assay involves Ca2+-regulated photoprotein obelin as a simple, sensitive and fast reporter. Applicability and the prospects of the approach were exemplified by identification of DNA aptamers to cardiac troponin I, a highly specific early biomarker for acute myocardial infarction. Two structurally different aptamers specific to various epitopes of troponin I were obtained and then tested in a model bioluminescent assay.
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Вид документа : Статья из журнала
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Автор(ы) : Simoncelli S., Kirillin G., Tolomeev A. P., Grossart H. -P.
Заглавие : A low-cost underwater particle tracking velocimetry system for measuring in situ particle flux and sedimentation rate in low-turbulence environments
Место публикации : Limnol. Oceanogr. Methods: Wiley Blackwell, 2019. - Article in press. - ISSN 15415856 (ISSN) , DOI 10.1002/lom3.10341
Аннотация: We describe a low-cost three-dimensional underwater particle tracking velocimetry system to directly measure particle settling rate and flux in low-turbulence aquatic environments. The system consists of two waterproof cameras that acquire stereoscopic videos of sinking particles at 48 frames s?1 over a tunable sampling volume of about 45 ? 25 ? 24 cm. A dedicated software package has been developed to allow evaluation of particle velocities, concentration and flux, but also of morphometric parameters such as particle area, sinking angle, shape irregularity, and density. Our method offers several advantages over traditional approaches, like sediment trap or expensive in situ camera systems: (1) it does not require beforehand particle collection and handling; (2) it is not subjected to sediment trap biases from turbulence, horizontal advection, or presence of swimmers, that may alter particulate load and flux; (3) the camera system enables faster data processing and flux computation at higher spatial resolution; (4) apart from the particle settling rates, the particle size distribution, and morphology is determined. We tested the camera system in Lake Stechlin (Germany) in low turbulence and mean flow, and analyzed the morphological properties and settling rates of particles to determine their sinking behavior. The particle flux assessed from conventional sediment trap measurements agreed well with that determined by our system. By this, the low-cost approach demonstrated its reliability in low turbulence environments and a strong potential to provide new insights into particulate carbon transport in aquatic systems. Extension of the method to more turbulent and advective conditions is also discussed. © 2019 The Authors. Limnology and Oceanography: Methods published by Wiley Periodicals, Inc. on behalf of Association for the Sciences of Limnology and Oceanography.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Murueva A. V., Shershneva A. M., Abanina K. V., Prudnikova S. V., Shishatskaya E. I.
Заглавие : Development and characterization of ceftriaxone-loaded P3HB-based microparticles for drug delivery
Место публикации : Dry. Technol.: Taylor and Francis Inc., 2018. - Article in press. - ISSN 07373937 (ISSN) , DOI 10.1080/07373937.2018.1487451
Ключевые слова (''Своб.индексиров.''): ceftriaxone--drug release--emulsification technique--microbiological evaluation--p3hb-microparticles--spray-drying--drying--emulsification--encapsulation--functional polymers--spray drying--targeted drug delivery--anti-bacterial activity--ceftriaxone--double emulsifications--drug release--encapsulation efficiency--micro-particles--microbiological evaluation--polymeric microparticles--controlled drug delivery
Аннотация: In this study, polymer-based microparticles are used to improve the therapeutic properties of ceftriaxone (CEF) and render them safer. Poly-3-hydroxybutyrate (P3HB) and poly-3-hydroxybutyrate/polyethylene glycol (P3HB-PEG)-based microparticles were prepared by two methods: a double emulsification technique and spray-drying. The microparticles were characterized in terms of size and zeta potential, morphology, total drug loading and drug release. The microparticles had spherical shapes with diameters of a size range from 0.74 to 1.55 µm (emulsification technique) and from 3.84 to 6.51 µm (spray-drying); CEF encapsulation efficiency was around 63% and 49% for these methods respectively. The CEF release from microparticles obtained by spray-drying reached 100% after 150 h, while for microparticles obtained by emulsification technique the total release of CEF did not exceed 34% after 312 h. The release profiles could be best explained by Zero order kinetics model, Higuchi and Korsmeyer-Peppas models, as the plots showed high linearity. Antibacterial activity of the microparticles was evaluated against gram-positive and gram-negative bacterial strains. In general, CEF encapsulation in polymeric microparticles preserves the therapeutic efficacy of the CEF and provides its prolonged effect. © 2018, © 2018 Taylor & Francis.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mogilnaya O. A., Ronzhin N. O., Bondar V. S.
Заглавие : Extracellular peroxidase activity and light emission of the mycelium of the basidiomycete neonothopanus nambi in the presence of ?-glucosidase
Место публикации : Biophysics: Pleiades Publishing, 2018. - Vol. 63, Is. 1. - С. 93-99. - ISSN 00063509 (ISSN) , DOI 10.1134/S0006350918010104
Ключевые слова (''Своб.индексиров.''): basidiomycetes--cell wall--luminescence--peroxidase--?-glucosidase
Аннотация: A comparative evaluation of the level of extracellular peroxidase activity and light-emission intensity of the mycelium of the luminescent basidiomycete Neonothopanus nambi in the presence of ?-glucosidase was performed. The enzyme activity damages the hyphae of the fungus leading to osmotic imbalance, partial degradation of the mycelium, and release of extracellular peroxidases into the incubation medium. The presence of ?-glucosidase reduces the time necessary to reach the maximum luminescence. Putative biochemical mechanisms that underlie the stimulation of reactive oxygen species formation (first and foremost, of hydrogen peroxide) in the N. nambi mycelium in the presence of ?-glucosidase are proposed. © O.A. Mogilnaya, N.O. Ronzhin, V.S. Bondar and Pleiades Publishing, Inc., 2018.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bashmakova E. E., Krasitskaya V. V., Bondar A. A., Eremina E. N., Slepov E. V., Zukov R. A., Frank L. A.
Заглавие : Bioluminescent SNP genotyping technique: Development and application for detection of melanocortin 1 receptor gene polymorphisms
Место публикации : Talanta: Elsevier B.V., 2018. - Vol. 189. - С. 111-115. - ISSN 00399140 (ISSN) , DOI 10.1016/j.talanta.2018.06.057
Ключевые слова (''Своб.индексиров.''): ca2+-regulated photoprotein obelin--genotyping--melanocortin 1 receptor gene--single nucleotide polymorphisms (snp)--bioluminescence--clinical research--curricula--diagnosis--genes--oncology--biomedical research--clinical characteristics--development and applications--genotyping--healthy individuals--photoproteins--receptor genes--single-nucleotide polymorphisms--dermatology
Аннотация: SNP genotyping based on the reaction of specific primer extension with the following bioluminescent detection of its products was shown to be potentially applicable for biomedical exploration. The paper describes its elaboration and first application in extensive biomedical research concerning MC1R gene variants’ frequency and associations with clinical characteristics in melanoma patients of Eastern Siberia (Krasnoyarsk region, Russia). Polymorphisms rs 1805007 (R151C), rs 1805008 (R160W), and rs 1805009 (D294H) were detected in 174 DNA samples from patients with histologically proved diagnosis of cutaneous melanoma and in 200 samples from healthy individuals. All the results on bioluminescent SNP genotyping were confirmed by Sanger sequencing. Some features characteristic of the population were found, i.e. melanoma is mostly associated with R160W or R151C while variant D294H is extremely rare; simultaneous carriage of any two investigated variants is also strongly associated with melanoma; R151C is associated with ulceration and consequently the disease course is more aggressive, etc. The design of the technique allows fast evaluation of any known diagnostically important SNP frequencies and associations across population. © 2018 Elsevier B.V.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev, Michail I., Sushchik, Nadezhda N., Tolomeev, Alexander P., Dgebuadze, Yury Yu
Заглавие : Meta-analysis of factors associated with omega-3 fatty acid contents of wild fish
Колич.характеристики :23 с
Коллективы : Russian Science Foundation [16-14-10001]
Место публикации : Rev. Fish. Biol. Fish.: SPRINGER, 2018. - Vol. 28, Is. 2. - С. 277-299. - ISSN 0960-3166, DOI 10.1007/s11160-017-9511-0. - ISSN 1573-5184(eISSN)
Примечания : Cited References:138. - The work was supported by a Russian Science Foundation Grant (No. 16-14-10001).
Предметные рубрики: FATTY-ACID-COMPOSITION
DIETARY DOCOSAHEXAENOIC ACID
LONG-CHAIN
Ключевые слова (''Своб.индексиров.''): docosahexaenoic acid--ecomorphological factors--eicosapentaenoic acid--nutritive value--phylogenetic factors
Аннотация: Fish are recognized as the main source of physiologically important omega-3 long-chain polyunsaturated fatty acids, namely, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), for human nutrition. However, muscle tissue contents of these fatty acids in diverse fish species, i.e., their nutritive value for humans, varied within two orders of magnitude. We reviewed contents of EPA and DHA, measured by similar methods using an internal standard during chromatography as mg per g of wet mass in 172 fish species belonging to 16 orders, to evaluate probable variations in phylogenetic and ecological drivers. EPA + DHA content varied from 25.6 mg g(-1) of wet mass (Sardinops sagax) to 0.12 mg g(-1) (Gymnura spp.). Multidimensional redundancy analysis revealed that among phylogenetic, ecomorphological and abiotic environmental factors, the highest proportion of variation contribution belonged to the shared contribution of sets of phylogenetic and ecomorphological factors. Specifically, the highest values of EPA + DHA content were characteristic of fish belonging to the orders Clupeiformes or Salmoniformes, were pelagic fast swimmers, ate zooplankton and inhabited marine waters or migrated from fresh to marine waters (anadromous migrations). High EPA and DHA content in muscle tissues of the above species appeared to be a metabolic adaptation for fast continuous swimming. In contrast to common beliefs, our meta-analysis did not support the significant influence of higher trophic levels (piscivory) and cold environments (homeoviscous adaptation) on EPA and DHA content in fish. However, many causes of high and low levels of physiologically important fatty acids in certain fish species remained unexplained and require evaluation in future studies.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shershneva, Anna, Murueva, Anastasiya, Nikolaeva, Elena, Shishatskaya, Ekaterina, Volova, Tatiana
Заглавие : Novel spray-dried PHA microparticles for antitumor drug release
Колич.характеристики :12 с
Коллективы : Russian Academy of Sciences [AAAA-A17- 117013050028-8]
Место публикации : Dry. Technol.: TAYLOR & FRANCIS INC, 2018. - Vol. 36, Is. 11. - С. 1387-1398. - ISSN 0737-3937, DOI 10.1080/07373937.2017.1407940. - ISSN 1532-2300(eISSN)
Примечания : Cited References:67. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences: [Project no. AAAA-A17- 117013050028-8].
Предметные рубрики: DRYING METHOD
IN-VITRO
BIODEGRADABLE NANOPARTICLES
ORAL
Ключевые слова (''Своб.индексиров.''): 5-fluorouracil--efficiency--microparticles--paclitaxel--polyhydroxyalkanoates--spray-drying
Аннотация: The production of poly-3-hydroxybutyrate (P3HB) and poly-3-hydroxybutyrate/polyethylene glycol (PEG)-based microparticles, loaded with antitumor drugs paclitaxel (PTX) and 5-Fluorouracil (5-FU) by spray-drying technique, was investigated. The average diameter of microparticles was found to be 3.4 +/- 0.5 pm and zeta potential was about -44 mV. The addition of surfactant PEG did not show any effect on the morphological characteristics of the particles. But the chemical structure of drug influenced on the properties. Microparticles had heterogeneous pores on the surface when the hydrophobic PTX was encapsulated. It was established that the addition of surfactant positively influenced on the properties of particles and led to the loading of 5-FU directly into the matrix. This is confirmed by the results of electron microscopy and dynamics of drug release in vitro. As a whole, the release profiles of PTX and 5-FU from composite P3HB/PEG microparticles were less than from P3HB microparticles. The results of the morphological evaluation of Hela cells demonstrated that the use of cytostatic drugs loaded in P3HB microparticles induces morphological changes associated with apoptosis (chromatin condensation, core fragmentation, margination of nucleus). Thus, the obtained results can serve as the basis for the development of new antitumor drugs of prolonged action, intended for various modes of administration.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Rota, Emilia, Martinsson, Svante, Erseus, Christer, Petushkov, Valentin N., Rodionova, Natalja S., Omodeo, Pietro
Заглавие : Green light to an integrative view of Microscolex phosphoreus (Duges, 1837) (Annelida: Clitellata: Acanthodrilidae)
Колич.характеристики :15 с
Коллективы : Russian Foundation for Basic Research [15-04-02695-a]; Adlerbert Foundation; [01201351504]
Место публикации : Zootaxa: MAGNOLIA PRESS, 2018. - Vol. 4496, Is. 1. - С. 175-189. - ISSN 1175-5326, DOI 10.11646/zootaxa.4496.1.13. - ISSN 1175-5334(eISSN)
Примечания : Cited References:53. - This work was partially supported by Grant 15-04-02695-a from the Russian Foundation for Basic Research and the state budget allocated to the fundamental research at the Russian Academy of Sciences (project No 01201351504). Funding for travel to Western Australia (CE, SM) was provided by the Adlerbert Foundation.
Предметные рубрики: BIOLUMINESCENT EARTHWORMS
LUMINOUS EARTHWORM
OLIGOCHAETA
HAPLOTYPES
Ключевые слова (''Своб.индексиров.''): peregrine earthworm--cryptic diversity--morphology--dna-barcoding--bioluminescence
Аннотация: The small synanthropic and peregrine earthworm Microscolex phosphoreus (Duges, 1837) is reported for the first time from Siberia. Morphological and DNA barcode (COI) analyses of this and widely separate samples worldwide demonstrate that, as currently identified, M. phosphoreus is a heterogeneous taxon, with divergent lineages occurring often in the same locality and hardly providing geographically structured genetic signals. The combined morphological and genetic evidence suggests that at least four of the found clades should be reclassified as separate species, both morphologically and genetically distinct from each other. However, as the specimen number was limited and only the COI gene was studied for the genetic work, we hesitate in formally describing new species. There would also be the problem of assigning the available names to specific lineages. Our findings encourage careful external and anatomical examination and using reliable characters such as the interchaetal distances and spermathecal morphology for correct identification and for deeper evaluation of cryptic diversity in this interesting bioluminescent worm.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petrova A. S., Lukonina A. A., Dementyev D. V., Bolsunovsky A. Ya. , Popov A. V., Kudryasheva N. S.
Заглавие : Protein-based fluorescent bioassay for low-dose gamma radiation exposures
Место публикации : Anal. Bioanal. Chem.: Springer Verlag, 2018. - ISSN 16182642 (ISSN) , DOI 10.1007/s00216-018-1282-5
Ключевые слова (''Своб.индексиров.''): bioassay--enzymes--fluorescence/luminescence--fluorescent protein--gamma radiation--radiotoxicity--efficiency--enzymes--fluorescence--gamma rays--proteins--proton transfer--fluorescence characteristics--fluorescence intensities--fluorescence spectra--fluorescence/luminescence--fluorescent protein--photochemical process--physiological liquids--radiotoxicity--bioassay
Аннотация: The study suggests an application of a coelenteramide-containing fluorescent protein (CLM-CFP) as a simplest bioassay for gamma radiation exposures. “Discharged obelin,” a product of the bioluminescence reaction of the marine coelenterate Obelia longissima, was used as a representative of the CLM-CFP group. The bioassay is based on a simple enzymatic reaction—photochemical proton transfer in the coelenteramide-apoprotein complex. Components of this reaction differ in fluorescence color, providing, by this, an evaluation of the proton transfer efficiency in the photochemical process. This efficiency depends on the microenvironment of the coelenteramide within the protein complex, and, hence, can evaluate a destructive ability of gamma radiation. The CLM-CFP samples were exposed to gamma radiation (137Cs, 2 mGy/h) for 7 and 16 days at 20 °C and 5 °C, respectively. As a result, two fluorescence characteristics (overall fluorescence intensity and contributions of color components to the fluorescence spectra) were identified as bioassay parameters. Both parameters demonstrated high sensitivity of the CLM-CFP-based bioassay to the low-dose gamma radiation exposure (up to 100 mGy). Higher temperature (20 °C) enhanced the response of CLM-CFP to gamma radiation. This new bioassay can provide fluorescent multicolor assessment of protein destruction in cells and physiological liquids under exposure to low doses of gamma radiation. [Figure not available: see fulltext.]. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
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11.

Вид документа : Статья из журнала
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Автор(ы) : Grodnitskaya I. D., Sorokin N. D., Evgrafova S. Y., Antonov G. I., Syrtsov S. N., Aleksandrov D. E., Trusova M. Y., Koroban N. V.
Заглавие : Microbial transformation of carbon CH4 and CO2 in permafrost-affected soils in tundra and forest ecosystems in Siberia
Место публикации : Russ. J. For. Sci.: Izdatel'stvo Nauka, 2017. - Is. 2. - С. 111-127. - ISSN 00241148 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacterial diversity--ch4 and co2 emission--dynamics and activity of microbial complexes--frost-crack polygons--larch forests--tundra--сryogenic soils
Аннотация: We studied structure, dynamics and functional (biogeochemical) activity of microbial complexes of cryogenic soils in larch forests in Central Evenkia and polygonal tundra on Samoilovskii Island, Lena Delta. We found that daily flux of methane from soil surface is 3–5 times less in forest soil than in the center of polygon in tundra. Short-term heating to 18.5–22.5 °C of permafrost-affected soil in larch forest caused sweetening of soil solution, shrinkage of eco-trophic groups of microorganisms and microbial biomass, as well as increase in greenhouse gases (CO2 and CH4) emission to the air. Notably the permafrost-affected soil on sandy deposits in tundra had highest microbial diversity of methanogenic archaea including Methanobacteriaceae, Methanomicrobiaceae, Methanosarcinaceae, Methanosaetaceae families. On the other hand only Methanosarcinacea were found in cryosols of larch forest. Both type I and type II methanotrophs were found in the forest soil, while only type II methanotrophs occurred in tundra soil. © 2017, Izdatel’stvo Nauka. All rights reserved.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petrova, Alena S., Lukonina, Anna A., Badun, Gennadii A., Kudryasheva, Nadezhda S.
Заглавие : Fluorescent coelenteramide-containing protein as a color bioindicator for low-dose radiation effects
Колич.характеристики :5 с
Коллективы : Russian Academy of Sciences [01201351504]; Russian Foundation for Basic Research [16-34-00695]
Место публикации : Anal. Bioanal. Chem.: SPRINGER HEIDELBERG, 2017. - Vol. 409, Is. 18. - С. 4377-4381. - ISSN 1618-2642, DOI 10.1007/s00216-017-0404-9. - ISSN 1618-2650(eISSN)
Примечания : Cited References:22. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences (project 01201351504) and by the Russian Foundation for Basic Research, Grant No. 16-34-00695.
Предметные рубрики: LUMINOUS MARINE-BACTERIA
DISCHARGED-OBELIN
AEQUORIN
Ключевые слова (''Своб.индексиров.''): fluorescent protein--coelenteramide--discharged photoprotein obelin--multicolor bioindicator--radiotoxicity
Аннотация: The study addresses the application of fluorescent coelenteramide-containing proteins as color bioindicators for radiotoxicity evaluation. Biological effects of chronic low-dose radiation are under investigation. Tritiated water (200 MBq/L) was used as a model source of low-intensive ionizing radiation of beta type. 'Discharged obelin,' product of bioluminescent reaction of marine coelenterate Obelia longissimi, was used as a representative of the coelenteramide-containing proteins. Coelenteramide, fluorophore of discharged obelin, is a photochemically active molecule; it produces fluorescence forms of different color. Contributions of 'violet' and 'blue-green' forms to the visible fluorescence serve as tested parameters. The contributions depend on the coelenteramide's microenvironment in the protein, and, hence, evaluate distractive ability and toxicity of radiation. The protein samples were exposed to beta radiation for 18 days, and maximal dose accumulated by the samples was 0.28 Gy, being close to a tentative limit of a low-dose interval. Increase of relative contribution of 'violet' fluorescence under exposure to the beta irradiation was revealed. High sensitivity of the protein-based test system to low-dose ionizing radiation (to 0.03 Gy) was demonstrated. The study develops physicochemical understanding of radiotoxic effects.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kudryasheva N. S., Petrova A. S., Dementyev D. V., Bondar A. A.
Заглавие : Exposure of luminous marine bacteria to low-dose gamma-radiation
Место публикации : J. Environ. Radioact.: Elsevier Ltd, 2017. - Vol. 169-170. - С. 64-69. - ISSN 0265931X (ISSN) , DOI 10.1016/j.jenvrad.2017.01.002
Ключевые слова (''Своб.индексиров.''): bioassay--low-dose gamma-radiation--luminous marine bacteria--mutagenic effect--radiotoxicity--temperature dependence--bacteria--bioassay--bioluminescence--gamma rays--ionizing radiation--irradiation--phosphorescence--physiological models--radiation effects--temperature distribution--low dose--marine bacterium--mutagenic effect--radiotoxicity--temperature dependence--radiation--bacteria (microorganisms)--photobacterium phosphoreum
Аннотация: The study addresses biological effects of low-dose gamma-radiation. Radioactive 137Cs-containing particles were used as model sources of gamma-radiation. Luminous marine bacterium Photobacterium phosphoreum was used as a bioassay with the bioluminescent intensity as the physiological parameter tested. To investigate the sensitivity of the bacteria to the low-dose gamma-radiation exposure (?250 mGy), the irradiation conditions were varied as follows: bioluminescence intensity was measured at 5, 10, and 20°С for 175, 100, and 47 h, respectively, at different dose rates (up to 4100 ?Gy/h). There was no noticeable effect of gamma-radiation at 5 and 10°С, while the 20°С exposure revealed authentic bioluminescence inhibition. The 20°С results of gamma-radiation exposure were compared to those for low-dose alpha- and beta-radiation exposures studied previously under comparable experimental conditions. In contrast to ionizing radiation of alpha and beta types, gamma-emission did not initiate bacterial bioluminescence activation (adaptive response). As with alpha- and beta-radiation, gamma-emission did not demonstrate monotonic dose-effect dependencies; the bioluminescence inhibition efficiency was found to be related to the exposure time, while no dose rate dependence was found. The sequence analysis of 16S ribosomal RNA gene did not reveal a mutagenic effect of low-dose gamma radiation. The exposure time that caused 50% bioluminescence inhibition was suggested as a test parameter for radiotoxicity evaluation under conditions of chronic low-dose gamma irradiation. © 2017 Elsevier Ltd
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mogilnaya O. A., Ronzhin N. O., Bondar V. S.
Заглавие : Comparative evaluation of total peroxidase and catalase activities during light emission of luminous fungus Neonothopanus nambi
Место публикации : Mycosphere: Guizhou Key Laboratory of Agricultural Biotechnology, 2016. - Vol. 7, Is. 4. - С. 499-510. - ISSN 20777000 (ISSN) , DOI 10.5943/mycosphere/7/4/9
Ключевые слова (''Своб.индексиров.''): basidiomycetes--hydrogen peroxide--luminescence--stress
Аннотация: Submerged cultivation of luminous fungus Neonothopanus nambi under orbital stirring causes formation of pellets with smooth or rough surfaces. The experiments showed that luminescence of the pellets washed in water increased considerably. Previous studies suggested possible participation of peroxidases in the light emitting reaction. In this study, oxidative azo coupling reaction accompanied by formation of chromogen was used to evaluate peroxidase activity in vivo, in brightly luminescent pellets and in pellets with low luminescence intensity (dim ones). Staining of the brightly luminescent pellets took a few minutes, and their staining intensity was several times higher than that of the dim pellets. From the results of in vivo experiments it was concluded that the bright pellets differed from the dim ones in the production of hydrogen peroxide, or, possibly, other peroxides. Measurements of total peroxidase and catalase activities in pellet extracts also showed an increase in enzyme activities along with an increase in luminescence intensity of native pellets. However, results of the in vitro experiments do not definitively suggest a direct relationship between luminescence and activity of these enzymes. We assume that luminescence of this fungal species may be an additional way to neutralize peroxide compounds under stress. © Guizhou Academy of Agricultural Sciences.
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15.

Вид документа : Статья из журнала
Шифр издания : 577.34/И 73
Автор(ы) : Болсуновский, Александр Яковлевич, Трофимова, Елена Александровна, Дементьев, Дмитрий Владимирович, Карпов, Антон Дмитриевич
Заглавие : Интенсивность накопления урана-238 представителями разных экологических уровней экосистемы р. Енисей
Коллективы : Институт биофизики Сибирского отделения Российской Академии наук (Красноярск)
Место публикации : Вестник Томского государственного университета. Биология. - Томск: Национальный исследовательский Томский государственный университет, 2016. - № 2 (34) . - С. 161-171. - DOI 10.17223/19988591/34/11
ISSN: 1998-8591
УДК : 577.34 + 597.5
Ключевые слова (''Своб.индексиров.''): коэффициент накопления--трофический уровень--philolimnogammarus viridis--fointinalis antipyretica--thymallus arcticus--esox lucius)
Аннотация: В результате многолетних исследований (2010-2013) получены данные по накоплению урана-238 в гидробионтах разных трофических уровней экосистемы р. Енисей. Пробы водного мха (Fointinalis antipyretica), зообентоса (Philolimnogammarus viridis и Ph. Cyaneus), сибирского хариуса (Thymallus arcticus) и щуки (Esox lucius) отбирали в районе ранее зарегистрированного повышенного содержания урана в воде р. Енисей (вблизи радиоактивных сбросов Горно-химического комбината Росатома). Показано, что уран способен эффективно накапливаться из воды в биомассе всех исследованных гидробионтов. Максимальные коэффициенты накопления (КН) урана получены для водного мха, что многократно превышает КН урана для зообентоса и мышечной ткани рыб. Средние значения КН урана для зообентоса статистически значимо превышают КН в мышцах рыб за весь период наблюдения. Не выявлено статистически значимых отличий в накоплении урана рыбами разного трофического уровня (хариус и щука)The aim of the research was a comparative evaluation of the intensity of uranium-238 accumulation in the biomass of aquatic organisms, occupying different trophic levels in the ecosystem of the Yenisei river. Previously, uranium-238 at concentrations higher than the background ones was detected in water and sediment samples of the Yenisei River collected close to the discharge site of one of the Rosatom facilities (the Mining-and-Chemical Combine). However, radioecological studies of the Yenisei River ecosystem have not focused on uranium isotopes in aquatic organisms so far. The toxic effects of uranium on living organisms are known to be based on its both radioactive and chemical properties. We conducted a study on accumulation of uranium-238 in aquatic organisms at different trophic levels in the Yenisei River ecosystem between 2010 and 2013. We collected samples of aquatic moss (Fointinalis antipyretica), zoobenthos (Philolimnogammarus viridis and Ph. cyaneus), arctic grayling (Thymallus arcticus), and pike (Esox lucius) from the Yenisei region at a distance of 85-90 km downstream of Krasnoyarsk (5-10 km downstream of the radioactive discharge of the Mining-and-Chemical Combine). Data on samples are shown in Table 1. We determined uranium concentration in the samples of aquatic organisms by neutron activation analysis, in water - by ICP-MS (Table 2). The study shows that uranium can be effectively concentrated from water and accumulate in the biomass of all aquatic organisms used in experiments. The highest uranium concentration factors (CFs) were obtained for aquatic moss, and they were several times higher than the uranium CFs for zoobenthos and fish muscles. Based on the uranium concentration from water, all aquatic organisms of the Yenisei River used in this study can be ranked as follows: aquatic moss gammarids grayling = pike. No statistically significant difference has been found between uranium accumulation by fish of different trophic levels (Arctic grayling and pike). The uranium CFs obtained in this study is comparable with the literature data on the CFs of artificial radionuclides for aquatic moss and fish of the Yenisei River
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Barkhatov Y. V., Tikhomirov A. A., Ushakova S. A., Shikhov V. N., Bartsev S. I., Degermendzhi A. G.
Заглавие : Experimental modeling of the influence of the rise in average summer temperatures on carbon circulation in tundra ecosystems
Место публикации : Dokl. Earth Sci.: Maik Nauka-Interperiodica Publishing, 2016. - Vol. 471, Is. 1. - С. 1168-1170. - ISSN 1028334X (ISSN) , DOI 10.1134/S1028334X16110106
Ключевые слова (''Своб.индексиров.''): carbon dioxide--ecology--landforms--carbon fluxes--climate condition--comparative evaluations--experimental modeling--physical simulation--soil respiration--summer temperature--temperature rise--ecosystems
Аннотация: A sealed vegetation chamber was designed and constructed for physical simulation of climate conditions in the Subarctic zone during the spring–summer time. The small laboratory tundra-simulating ecosystem (TSE) was created for comparative evaluation of the rates of soil respiration and of the total balance of carbon fluxes in tundra ecosystems. The test experiment was performed to study the TSE response to a temperature rise in air and soil by 2°C in terms of the intensity of the СО2 flux. It was shown that this increase in temperature would cause a pronounced shift in the balance of СО2 production and utilization in the ecosystem from near-zero values to a stable generation of 24 ?mol/h of CO2 per 1 kg of dry biomass. © 2016, Pleiades Publishing, Ltd.
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17.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Kukoba N., Ivanova Y., Botvich I.
Заглавие : The influence of altitudinal zonality on spectral characteristics (MODIS/Terra) mountain forests of Western Sayan
Место публикации : Climate Change Impacts on High-Altitude Ecosystems . - 2015. - С. 637-643. - ISBN 9783319128597 (ISBN); 9783319128580 (ISBN), DOI 10.1007/978-3-319-12859-7_26
Ключевые слова (''Своб.индексиров.''): mountain forests of siberia--net primary production (npp)--vegetation indices
Аннотация: The purpose of this study is to find a relationship between changes in spectral characteristics (MODIS/Terra) of mountain forests and the altitude at which they grow. In the study area, which is located in the West Sayan Mountains (in South Siberia), the types of forest ecosystems change markedly with altitude. The study uses the data of the MODIS-NPP model intended for the evaluation of global net production. Results of the study show that the best approach to dividing mountain forests into different types is to use 8-day composites of satellite data collected at the beginning of the growing season (April-May). This is the time when the most significant differences are recorded between vegetation indices, including Normalized Difference Vegetation Index (NDVI), LAI, and EVI of the mountain forests growing in different altitudinal zones. © Springer International Publishing Switzerland 2015.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kudryasheva, N. S., Rozhko, T. V.
Заглавие : Effect of low-dose ionizing radiation on luminous marine bacteria: radiation hormesis and toxicity
Колич.характеристики :10 с
Коллективы : Russian Foundation for Basic Research [13-04-01305a]; Program "Molecular and Cellular Biology" of the Russian Academy of Sciences [VI 57.1.1]; Russian Science Foundation [14-14-00076]
Место публикации : J. Environ. Radioact.: ELSEVIER SCI LTD, 2015. - Vol. 142. - С. 68-77. - ISSN 0265-931X, DOI 10.1016/j.jenvrad.2015.01.012. - ISSN 1879-1700(eISSN)
Примечания : Cited References:131. - This work was supported by the Russian Foundation for Basic Research, Grant No.13-04-01305a, the Program "Molecular and Cellular Biology" of the Russian Academy of Sciences, project VI 57.1.1. The part of the work (review of effects of americium-241) was supported by the Russian Science Foundation, Grant No. 14-14-00076.
Предметные рубрики: RECOMBINANT LUMINESCENT MICROORGANISMS
PHOTOBACTERIUM-LEIOGNATHI
Ключевые слова (''Своб.индексиров.''): marine bacteria--low-dose effects--radiation hormesis--radiotoxicity--reactive oxygen species
Аннотация: The paper summarizes studies of effects of alpha- and beta-emitting radionuclides (americium-241, uranium-235+238, and tritium) on marine microorganisms under conditions of chronic low-dose irradiation in aqueous media. Luminous marine bacteria were chosen as an example of these microorganisms; bioluminescent intensity was used as a tested physiological parameter. Non-linear dose-effect dependence was demonstrated. Three successive stages in the bioluminescent response to americium-241 and tritium were found: 1 - absence of effects (stress recognition), 2 - activation (adaptive response), and 3 - inhibition (suppression of physiological function, i.e. radiation toxicity). The effects were attributed to radiation hormesis phenomenon. Biological role of reactive oxygen species, secondary products of the radioactive decay, is discussed. The study suggests an approach to evaluation of non-toxic and toxic stages under conditions of chronic radioactive exposure. (C) 2015 Elsevier Ltd. All rights reserved.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bolsunovsky A.Y., Zotina T.A., Medvedeva M.Y., Trofimova E.A.
Заглавие : Evaluation of cytogenetic abnormalities in cells of submerged plant Elodea canadensis in the Yenisei River section affected by industrial pollution: Field studies and laboratory experiments
Место публикации : Dokl. Biol. Sci. - 2015. - Vol. 460, Is. 1. - С. 27-31. - ISSN 00124966 (ISSN) , DOI 10.1134/S0012496615010044
Ключевые слова (''Своб.индексиров.''): elodea canadensis
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20.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Ivanova Y., Soukhovolsky V.
Заглавие : Net primary production in forest ecosystem of middle siberia: Assessment using a model of tree component phytomass distribution
Место публикации : Climate Change Impacts on High-Altitude Ecosystems . - 2015. - С. 627-635. - ISBN 9783319128597 (ISBN); 9783319128580 (ISBN), DOI 10.1007/978-3-319-12859-7_25
Ключевые слова (''Своб.индексиров.''): forest stands of middle siberia--net primary production (npp)--root phytomass
Аннотация: The authors propose several methods of net primary production (NPP) evaluation for tree stands of Middle Siberia. The authors’ approach is based on the model of the distribution of tree (tree stand) phytomass among different tree components (stem, roots, branches, foliage) using the Zipf-Pareto equation. This model can be used not only to calculate the phytomass and NPP of the tree aboveground parts but also to determine quite accurately the phytomass and NPP of the roots. Several approaches to calculation of tree stand NPP have been proposed, depending on the amount of available data. © Springer International Publishing Switzerland 2015.
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