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The mathematical theory of population waves (Russian) / A. G. Degermendzhy, N. S. Pechurkin, I. A. Terskov // Studia Biophysica. - 1974. - Vol. 43, Is. 1. - С. 25-40 . - ISSN 0081-6337
Кл.слова (ненормированные):
evolution -- mathematical model -- model -- population explosion -- population model -- theoretical study

Scopus
Держатели документа:
Dept. Biophys., Inst. Phys., Siberian Branch, Acad. Sci. USSR, Krasnoyarsk, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Degermendzhy, A.G.; Pechurkin, N.S.; Terskov, I.A.

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Comparative analysis of the evolution of Eigen hypercycles and populations of microorganisms in open systems / N. S. Pechurkin, N. V. Nikiforova, A. G. Degermendzhi // Biophysics. - 1982. - Vol. 27, Is. 2. - P304-311 . - ISSN 0006-3509
Аннотация: Models of the evolution of Eigen's hypercycles and microbial flow populations are compared. It is shown that these model descriptions of microevolution completely agree in the case of maintenance of constant organization. In the case of constant flows in the Eigen model it is necessary to make additional allowance for the dynamics of the concentrations of the energy monomers. The results of the experiments on the micro-evolution of microbes in these and other conditions are presented. В© 1982.

Scopus
Держатели документа:
Kirenskii Institute of Physics, Siberian Division, U.S.S.R. Academy of Sciences, Krasnoyarsk, Russian Federation
Krasnoyarsk State University, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pechurkin, N.S.; Nikiforova, N.V.; Degermendzhi, A.G.

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COMPARATIVE-ANALYSIS OF THE EVOLUTION OF EIGENS HYPERCYCLES AND MICROBIAL-POPULATIONS IN OPEN SYSTEMS [Текст] / N. S. PECHURKIN, N. V. NIKIFOROVA, A. G. DEGERMENDZY // Biofizika. - 1982. - Vol. 27, Is. 2. - С. 297-303. - Cited References: 10 . - 7. - ISSN 0006-3029

РУБ Biophysics

Держатели документа:
KRASNOYARSK STATE UNIV,KRASNOYARSK,USSR : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
PECHURKIN, N.S.; NIKIFOROVA, N.V.; DEGERMENDZY, A.G.

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Isolation of bioluminescent functions from Photobacterium leiognathi: analysis of luxA, luxB, luxG and neighboring genes / B. A. Illarrionov [et al.] // Gene. - 1990. - Vol. 86, Is. 1. - P89-94 . - ISSN 0378-1119
Кл.слова (ненормированные):
Bioluminescence -- expression in E. coli -- luciferase -- molecular evolution -- nucleotide sequence -- protein alignment -- recombinant DNA -- luciferase -- amino acid sequence -- article -- bioluminescence -- fungus -- gene structure -- genetic engineering -- heredity -- nonhuman -- nucleotide sequence -- priority journal -- vibrionaceae -- Acyltransferases -- Amino Acid Sequence -- Bacterial Proteins -- Base Sequence -- Cloning, Molecular -- DNA, Bacterial -- Genes, Structural, Bacterial -- Luciferase -- Luminescence -- Molecular Sequence Data -- Operon -- Photobacterium -- Restriction Mapping -- Escherichia coli -- Fungi -- Photobacterium leiognathi -- Vibrio harveyi -- Vibrionaceae
Аннотация: Genes encoding luminescence of Photobacterium leiognathi have been cloned in Escherichia coli. The luminescent clones were readily apparent. Among them, a clone containing a recombinant plasmid with a 13.5-kb insertion was identified. This DNA fragment contained all of the luminescence-encoding genes. The luciferase-encoding genes (lux) in this DNA fragment were localized. We have sequenced a part of the cloned lux region and identified the luxA, luxB and luxG genes encoding the ? and ? subunits of luciferase and a ? protein with an Mr of 26 180, respectively. The analysis of deduced amino acid sequences and comparison with known luciferase sequences from Vibrio harveyi, indicate the common origin of these proteins. В© 1990.

Scopus
Держатели документа:
Krasnoyarsk State University, Krasnoyarsk, 660062, Russian Federation
All-Union Research Institute of Molecular Biology, Novosibirsk Region, 633159, Russian Federation
Institute of Biophysics, Krasnoyarsk, 660036, Russian Federation
Institute of Clinical and Experimental Medicine, Novosibirsk, Russian Federation
Novosibirsk Institute of Bioorganic Chemistry, Novosibirsk, 630090, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Illarrionov, B.A.; Blinov, V.M.; Douchenko, A.P.; Protopopova, M.V.; Karginov, V.A.; Mertvetsov, N.P.; Gitelson, J.I.

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THE COMPARATIVE REDUNDANCY OF GENES OF VARIOUS ORGANISMS AND VIRUSES [Текст] / A. N. GORBAN [и др.] // Genetika. - 1993. - Vol. 29, Is. 9. - P. 1413-1419. - Cited References: 20 . - ISSN 0016-6758

РУБ Genetics & Heredity
Рубрики:
CODON
Аннотация: This paper is devoted to the comparative stude of redundancy of genetic texts of various organisms and viruses. To determine the tedundance of a gene, we have introduced the strict measure for that latter. The measure for a text's redundance is the length of restriction of Frequence/Correlation Dictionary of a given genetic text. Frequence/Correlation Dictionary is the ser of all subsequences belang to a given genetic text, accompanied by the frequencies of their occurrence. The restriction length is defined as that one, for which all the subsequences (of that length) are unique. We have found, that genes of human viruses are less redundant, in comparison to those of human genes. Other aspects of a comparative redundance invastigations of the genes are discussed. The problem of the determination of truet intron could be treated by this methodology, as well, as the evolution of genome.

WOS
Держатели документа:
RUSSIAN ACAD SCI,CTR COMP,KRASNOYARSK,RUSSIA
ИВМ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
GORBAN, A.N.; MIRKES, E.M.; POPOVA, T.G.; SADOVSKY, M.G.

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The comparative redundancy of genes of various organisms and viruses / A. N. Gorban [и др.] // Genetika. - 1993. - Vol. 29, Is. 9. - P. 1413-1419 . - ISSN 0016-6758
Кл.слова (ненормированные):
article -- dna content -- evolution -- genome -- nonhuman -- restriction mapping -- virus -- amino acid sequence -- comparative study -- gene frequency -- human -- molecular genetics -- nucleotide sequence -- restriction mapping -- virus gene -- Amino Acid Sequence -- Base Sequence -- Comparative Study -- English Abstract -- Gene Frequency -- Genes, Viral -- Human -- Molecular Sequence Data -- Restriction Mapping

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gorban, A.N.; Mirkes, E.M.; Popova, T.G.; Sadovsky, M.G.

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Multi-satellite data merge to combine NOAA AVHRR efficiency with Landsat-6 MSS spatial resolution to study vegetation dynamics [Text] / A. . Shevyrnogov, P. . Trefois, G. . Vysotskaya ; ed. , P Trefo // REMOTE SENSING FOR LAND SURFACE CHARACTERISATION. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON PRESS LTD, 2000. - Vol. 26: A3 1 and A3 2 Symposia of COSPAR Scientific Commission A held at the 32nd COSPAR Scientific Assembly (JUL 12-19, 1998, NAGOYA, JAPAN), Is. 7. - P. 1131-1133, DOI 10.1016/S0273-1177(99)01130-8. - Cited References: 2 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Remote Sensing

Аннотация: Spectral range and good replication provided by NOAA AVHRR data make possible to study evolution of vegetation cover and temperature in time. However, the spatial resolution even of 1000 m does not allow accurate location of the changes. The new developed NOAA NDVI image provides better apparent spatial resolution by the following procedure: - resample the NOAA image to 100 m pixel size; - georeference both NOAA and MSS (Landsat-6) to common UTM coordinates; - extract the brightness component from MSS (using the first principal component); - merge NOAA. NDVI and MSS brightness images. The resulting image features the color of NOAA NDVI and topographic details of MSS intensity. This work offers relevant techniques to extract information from satellite imagery to apply to plant dynamics investigation. (C) 2000 COSPAR. Published by Elsevier Science Ltd.

WOS
Держатели документа:
RAS, SB, Inst Biophys, Krasnoyarsk 660036, Russia
Royal Museum Cent Africa, Brussels, Belgium
RAS, SB, Inst Computat Modelling, Krasnoyarsk 660036, Russia
ИБФ СО РАН
ИВМ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shevyrnogov, A...; Trefois, P...; Vysotskaya, G...; Trefo, , P \ed.\

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Evaluation of the effect of light intensity on the measurement of the photosynthetic rate in plankton microalgae by the chlorophyll fluorescence method [Text] / N. A. Gaevskii [et al.] // Russ. J. Plant Physiol. - 2000. - Vol. 47, Is. 6. - P. 820-825, DOI 10.1023/A:1026671531500. - Cited References: 21 . - ISSN 1021-4437

РУБ Plant Sciences
Рубрики:
QUANTUM YIELDS
   PHYTOPLANKTON
   CHLOROPLASTS
   ALGAE
Кл.слова (ненормированные):
fluorescence -- chlorophyll -- microalgae -- photosynthetic rate -- photosynthetic activity
Аннотация: The use of relative variable fluorescence (RVF) of chlorophyll, as measured in the presence of Diuron, an inhibitor of electron transfer, for the estimation of the photosynthetic activity of plankton microalgae was analyzed under a wide range of light intensities in the PAR region. Oxygen evolution rates (estimated by the method of light and dark bottles and the amperometric method), RVF, and chlorophyll a concentration were measured in parallel in natural algal cenoses and microecosystems. When the previously used regression equation, in the form A = b(DeltaF/F-d)CchlI, where A is O-2 evolution rate (g/(m(3) h), DeltaF/F-d is RVF (relative units), C-chl is chlorophyll a concentration (mg/m(3)), and I is light intensity (W/m(2)), was verified in the PAR region, we observed a nonlinear dependence of the correction coefficient b on I, which can be described by the formula b = 6.227 x 10(3)rootI. This result agrees with the hypothesis that chlorophyll a fluorescence quenching comprises photochemical (qQ) and energy (qE) components. On the basis of the energy model, we determined the upper limit b(max) = 0.003 for light intensity range I < 4.4 W/m(2) and the lower limit b(min) = 0.0003 for I = 400 W/m(2).

WOS
Держатели документа:
Krasnoyarsk State Univ, Dept Biol, Krasnoyarsk 660036, Russia
Russian Acad Sci, Siberian Div, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gaevskii, N.A.; Kolmakov, V.I.; Popel'nitskii, V.A.; Gold, V.M.; Dubovskaya, O.P.

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Multi-satellite data merge to combine NOAA AVHRR efficiency with landsat-6 MSS spatial resolution to study vegetation dynamics / A. Shevyrnogov, P. Trefois, G. Vysotskaya // Advances in Space Research. - 2000. - Vol. 26, Is. 7. - P1131-1133, DOI 10.1016/S0273-1177(99)01130-8 . - ISSN 0273-1177
Кл.слова (ненормированные):
AVHRR -- Landsat multispectral scanner -- NOAA satellite -- satellite data -- spatial resolution -- vegetation dynamics
Аннотация: Spectral range and good replication provided by NOAA AVHRR data make possible to study evolution of vegetation cover and temperature in time. However, the spatial resolution even of 1000 m does not allow accurate location of the changes. The new developed NOAA NDVI image provides better apparent spatial resolution by the following procedure: - resample the NOAA image to 100 m pixel size; - georeference both NOAA and MSS (Landsat-6) to common UTM coordinates; - extract the brightness component from MSS (using the first principal component); - merge NOAA NDVI and MSS brightness images. The resulting image features the color of NOAA NDVI and topographic details of MSS intensity. This work offers relevant techniques to extract information from satellite imagery to apply to plant dynamics investigation. (C) 2000 COSPAR. Published by Elsevier Science Ltd.

Scopus
Держатели документа:
Institute of Biophysics of SB RAS, 660036, Krasnoyarsk, Russian Federation
Royal Museum of Central Africa, Brussels, Belgium
Inst. of Compl. Modelling SB RAS, 660036, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shevyrnogov, A.; Trefois, P.; Vysotskaya, G.

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10.

Preparation and X-ray crystallographic analysis of recombinant obelin crystals diffracting to beyond 1.1 angstrom [Text] / E. S. Vysotski [et al.] // Acta Crystallogr. Sect. D-Biol. Crystallogr. - 2001. - Vol. 57. - P1919-1921, DOI 10.1107/S0907444901016523. - Cited References: 16 . - ISSN 0907-4449

РУБ Biochemical Research Methods + Biochemistry & Molecular Biology + Biophysics + Crystallography
Рубрики:
PHOTOPROTEIN AEQUORIN
   LONGISSIMA
   EVOLUTION
   CDNA
Аннотация: Crystals of recombinant obelin, the Ca2+-regulated photoprotein from the marine hydroid Obelia longissima, have been grown from a solution containing PEG 8000 and potassium phosphate. Hexamine-cobalt trichloride was used as an additive to increase the chance of crystallization. The crystals grow in a light yellow cubic form (0.5 x 0.5 x 0.45 mm) which diffracts to beyond 1.1 Angstrom resolution. The crystals belong to the space group C2, with unit-cell parameters a=83.43, b=54.92, c=52.99 Angstrom, beta = 112.00 degrees. The asymmetric unit contains one molecule. Crystals exposed to calcium ion before and after X-ray irradiation emit light, confirming that the crystals consist of an active photoprotein.

Держатели документа:
Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA
Russian Acad Sci, Siberian Branch, Inst Biophys, Photobiol Lab, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Vysotski, E.S.; Liu, Z.J.; Rose, J...; Wang, R.C.; Lee, J...

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11.

Life as a set of matter transformation cycles: Ecological attributes of life [Text] / S. I. Bartsev, V. V. Mezhevikin, V. A. Okhonin ; ed. Y Mogami [et al.] // SPACE LIFE SCIENCES: LIVING ORGANISMS, BIOLOGICAL PROCESSES AND THE LIMITS OF LIFE. Ser. ADVANCES IN SPACE RESEARCH : ELSEVIER SCIENCE BV, 2001. - Vol. 28: F1 4/F4 5/and F3 2/F3 3 Symposium of COSPAR Scientific Commission F held at the 33rd COPAR Scientific Assembly (JUL 16-23, 2000, WARSAW, POLAND), Is. 4. - P. 607-612, DOI 10.1016/S0273-1177(01)00389-1. - Cited References: 4 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences

Аннотация: An approach to searching for extraterrestrial life on the base of "autotroph" concept of the origin of life is presented in the paper, According to this concept the origin of life took place in three stages. The first stage was developed inside the global geochemical cycle in which the turnover of different chemical transformations was implemented by solar radiation and/or heat energy of bowels of the Earth. At the second stage, after the autocatalytic systems have emerged these systems evolved as a result of "natural selection" by autocatalysis parameters up to emergence of special inheritance systems that drastically improved the autocatalysis parameters. The best in terms of autocatalysis parameters were the autocatalysis systems based on phase-separated particles where complex structures can form not only on the basis of covalent interactions. Such autocatalysis systems can emerge only in liquid in a certain range of temperatures and pressures. At this stage the geochemical cycle complicated involving new substances. At the third stage the evolution involved improvement of inheritance systems resulting in formation of the modern type of genetic apparatus. This concept formed the basis to consider approaches to experimental modeling of major aspects of the origin of life and to outlining some general features of life that can extend the sensitive horizon of searching for extraterrestrial life. (C) 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bartsev, S.I.; Mezhevikin, V.V.; Okhonin, V.A.; Mogami, Y \ed.\; Bruce, L \ed.\; Nechitailo, G \ed.\; Kondyurin, A \ed.\; Clark, BC \ed.\

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12.

Computer modeling of the biotic cycle formation in a closed ecological system / A. V. Brilkov [et al.] // Advances in Space Research. - 2001. - Vol. 27, Is. 9. - P1587-1592, DOI 10.1016/S0273-1177(01)00253-8 . - ISSN 0273-1177
Кл.слова (ненормированные):
Ecology -- Energy utilization -- Mathematical models -- Biotic turnover -- Predators -- Space research -- artificial ecosystem -- alga -- animal -- article -- biological model -- computer simulation -- Cyprinodontiformes -- Daphnia -- ecosystem -- energy metabolism -- evolution -- food chain -- microclimate -- plankton -- Algae -- Animals -- Computer Simulation -- Daphnia -- Ecological Systems, Closed -- Ecosystem -- Energy Metabolism -- Evolution -- Food Chain -- Models, Biological -- Plankton -- Poecilia
Аннотация: The process of biotic turnover in a closed ecological system (CES) with an external energy flow was analyzed by mathematical modeling of the biotic cycle formation. The formation of hierarchical structure in model CESs is governed by energy criteria. Energy flow through the ecosystem increases when a predator is introduced into a "producer-reducer" system at steady state. Analysis of the model shows that under certain conditions the presence of the primary predator with its high mineralization ability accelerates the biotic turnover measured by primary production. We, therefore, conclude that for every system it is possible to find a suitable predator able to provide the system with a higher biotic turnover rate and energy consumption. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Brilkov, A.V.; Ganusov, V.V.; Morozova, E.V.; Pechurkin, N.S.

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13.

Volatile metabolites and external CO2 exchange of wheat cenoses under optimal conditions and thermal stress [Text] / I. I. Gitel'son [et al.] // Appl. Biochem. Microbiol. - 2002. - Vol. 38, Is. 1. - P. 78-82, DOI 10.1023/A:1013212907872. - Cited References: 17 . - ISSN 0003-6838

РУБ Biotechnology & Applied Microbiology + Microbiology
Рубрики:
EMISSIONS
Аннотация: The effects of elevated temperature (35 and 45degreesC) on photosynthesis, respiration, and both the qualitative and quantitative compositions of volatile emissions (VE) of wheat (Triticum aestuvum L. cultivar 232) cenoses at light intensities of 70, 150, or 240 W/m(2) of photosynthetically available radiation (PAR) were studied. At a PAR of 240 W/m(2), the thermal stabilities of photosynthesis and respiration increased at 35degreesC and decreased at 45degreesC. Elevated temperatures nonuniformly changed the rates and direction of VE syntheses. In this process, the highest increase in VE evolution was observed at 70 W/m(2) and 35degreesC; the lowest, at 240 W/m(2). In addition, the concentrations and composition of VE during the repair period differed from the initial values.

WOS
Держатели документа:
Russian Acad Sci, Siberian Div, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitel'son, I.I.; Tikhomirov, A.A.; Parshina, O.V.; Ushakova, S.A.; Kalacheva, G.S.

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14.

Volatile metabolites and external CO2 exchange of wheat cenoses under optimal conditions and thermal stress / I. I. Gitel'son [et al.] // Applied Biochemistry and Microbiology. - 2002. - Vol. 38, Is. 1. - P78-82, DOI 10.1023/A:1013212907872 . - ISSN 0003-6838
Кл.слова (ненормированные):
carbon dioxide -- volatile agent -- article -- biosynthesis -- carbon dioxide transport -- chemical composition -- concentration (parameters) -- controlled study -- cultivar -- metabolite -- nonhuman -- photosynthesis -- photosynthetically active radiation -- plant metabolism -- qualitative analysis -- quantitative analysis -- stress -- temperature sensitivity -- thermal exposure -- thermostability -- wheat -- Rickettsia sp. PAR -- Triticum -- Triticum aestivum
Аннотация: The effects of elevated temperature (35 and 45В°C) on photosynthesis, respiration, and both the qualitative and quantitative compositions of volatile emissions (VE) of wheat (Triticum aestuvum L. cultivar 232) cenoses at light intensities of 70, 150, or 240 W/m2 of photosynthetically available radiation (PAR) were studied. At a PAR of 240 W/m2, the thermal stabilities of photosynthesis and respiration increased at 35В°C and decreased at 45В°C. Elevated temperatures nonuniformly changed the rates and direction of VE syntheses. In this process, the highest increase in VE evolution was observed at 70 W/m2 and 35В°C; the lowest, at 240 W/m2. In addition, the concentrations and composition of VE during the repair period differed from the initial values.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitel'son, I.I.; Tikhomirov, A.A.; Parshina, O.V.; Ushakova, S.A.; Kalacheva, G.S.

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15.

Volatile Metabolites and External CO2 Exchange of Wheat Cenoses under Optimal Conditions and Thermal Stress / I. I. Gitel'zon [и др.] // Prikladnaya Biokhimiya i Mikrobiologiya. - 2002. - Vol. 38, Is. 1. - С. 95 . - ISSN 0555-1099
Аннотация: The effects of elevated temperature (35 and 45В°C) on photosynthesis, respiration, and both the qualitative and quantitative compositions of volatile emissions (VE) of wheat (Triticum aestuvum L. cultivar 232) cenoses at light intensities of 70, 150, or 240 W/m2 of photosynthetically available radiation (PAR) were studied. At a PAR of 240 W/m2, the thermal stabilities of photosynthesis and respiration increased at 35В°C and decreased at 45В°C. Elevated temperatures nonuniformly changed the rates and direction of VE syntheses. In this process, the highest increase in VE evolution was observed at 70 W/m2; the lowest, at 240 W/m2 and 35В°C. In addition, the concentrations and composition of VE during the repair period differed from the initial values.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitel'zon, I.I.; Tikhomirov, A.A.; Parshina, O.V.; Ushakova, S.A.; Kalacheva, G.S.

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16.

Violet bioluminescence and fast kinetics from W92F obelin: Structure-based proposals for the bioluminescence triggering and the identification of the emitting species [Text] / E. S. Vysotski [et al.] // Biochemistry. - 2003. - Vol. 42, Is. 20. - P6013-6024, DOI 10.1021/bi027258h. - Cited References: 45 . - ISSN 0006-2960

РУБ Biochemistry & Molecular Biology
Рубрики:
RAY CRYSTALLOGRAPHIC ANALYSIS
   PHOTOPROTEIN AEQUORIN
   ANGSTROM RESOLUTION
   RECOMBINANT OBELIN
   CALCIUM
   LUMINESCENCE
   LONGISSIMA
   EVOLUTION
   PROTEINS
   COELENTERAZINE
Аннотация: Obelin from the hydroid Obelia longissima and aequorin are members of a subfamily of Ca2+-regulated photoproteins that is a part of the larger EF-hand calcium binding protein family. On the addition of Ca2+, obelin generates a blue bioluminescence emission (lambda(max) = 485 nm) as the result of the oxidative decarboxylation of the bound substrate, coelenterazine. The W92F obelin mutant is noteworthy because of the unusually high speed with which it responds to sudden changes of [Ca2+] and because it emits violet light rather than blue due to a prominent band with lambda(max) = 405 nm. Increase of pH in the range from 5.5 to 8.5 and using D2O both diminish the contribution of the 405 nm band, indicating that excited state proton transfer is involved. Fluorescence model studies have suggested the origin of the 485 nm emission as the excited state of an anion of coelenteramide, the bioluminescence reaction product, and 405 nm from the excited neutral state. Assuming that the dimensions of the substrate binding cavity do not change during the excited state formation, a His22 residue within hydrogen bonding distance to the 6-(p-hydroxy)-phenyl group of the excited coelenteramide is a likely candidate for accepting the phenol proton to produce an ion-pair excited state, in support of recent suggestions for the bioluminescence emitting state. The proton transfer could be impeded by removal of the Trp92 H-bond, resulting in strong enhancement of a 405 nm band giving the violet color of bioluminescence. Comparative analysis of 3D structures of the wild-type (WT) and W92F obelins reveals that there are structural displacements of certain key Ca2+-ligating residues in the loops of the two C-terminal EF hands as well as clear differences in hydrogen bond networks in W92F. For instance, the hydrogen bond between the side-chain oxygen atom of Asp 169 and the main-chain nitrogen of Arg112 binds together the incoming alpha-helix of loop III and the exiting cc-helix of loop IV in WT, providing probably concerted changes in these EF hands on calcium binding. But this linkage is not found in W92F obelin. These differences apparently do not change the overall affinity to calcium of W92F obelin but may account for the kinetic differences between the WT and mutant obelins. From analysis of the hydrogen bond network in the coelenterazine binding cavity, it is proposed that the trigger for bioluminescence reaction in these Ca2+-regulated photoproteins may be a shift of the hydrogen bond donor-acceptor separations around the coelenterazine-2-hydroperoxy substrate, initiated by small spatial adjustment of the exiting a-helix of loop IV.

Держатели документа:
Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA
Univ Georgia, Dept Chem, Athens, GA USA
RAS, SB, Photobiol Lab, Inst Biophys, Krasnoyarsk, Russia
Univ Washington, Friday Harbor Labs, Seattle, WA 98195 USA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Vysotski, E.S.; Liu, Z.J.; Markova, S.V.; Blinks, J.R.; Deng, L...; Frank, L.A.; Herko, M...; Malikova, N.P.; Rose, J.P.; Wang, B.C.; Lee, J...

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17.

Mass exchange in an experimental new-generation life support system model based on biological regeneration of environment [Text] / A. A. Tikhomirov [et al.] ; ed. M Nelson [et al.] // SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - P. 1711-1720, DOI 10.1016/S0273-1177(03)00108-X. - Cited References: 13 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Ecology + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences

Аннотация: An experimental model of a biological life support system was used to evaluate qualitative and quantitative parameters of the internal mass exchange. The photosynthesizing unit included the higher plant component (wheat and radish), and the heterotrophic unit consisted of a soil-like substrate, California worms, mushrooms and microbial microflora. The gas mass exchange involved evolution of oxygen by the photosynthesizing component and its uptake by the heterotroph component along with the formation and maintaining of the SLS structure, growth of mushrooms and California worms, human respiration, and some other processes. Human presence in the system in the form of "virtual human" that at regular intervals took part in the respirative gas exchange during the experiment. Experimental data demonstrated good oxygen/carbon dioxide balance, and the closure of the cycles of these gases was almost complete. The water cycle was nearly 100% closed. The main components in the water mass exchange were transpiration water and the watering solution with mineral elements. Human consumption of the edible plant biomass (grains and roots) was simulated by processing these products by a unique physicochemical method of oxidizing them to inorganic mineral compounds, which were then returned into the system and fully assimilated by the plants. The oxidation was achieved by "wet combustion" of organic biomass, using hydrogen peroxide following a special procedure, which does not require high temperature and pressure. Hydrogen peroxide is produced from the water inside the system. The closure of the cycle was estimated for individual elements and compounds. Stoichiometric proportions are given for the main components included in the experimental model of the system. Approaches to the mathematical modeling of the cycling processes are discussed, using the data of the experimental model. Nitrogen, as a representative of biogenic elements, shows an almost 100% closure of the cycle inside the system, The proposed experimental model of a biological system is discussed as a candidate for potential application in the investigations aimed at creating ecosystems with largely closed cycles of the internal mass exchange. The formation and maintenance of sustainable cycling of vitally important chemical elements and compounds in biological life support systems (BLSS) is an extremely pressing problem. To attain the stable functioning of biological life support systems (BLSS) and to maintain a high degree of closure of material cycles in them, it is essential to understand the character of mass exchange processes and stoichiometric proportions of the initial and synthesized components of the system. (C) 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Krasnoyarsk, Russia
Univ Strasbourg 1, Clermont Ferrand, France
ESA, Estec, Environm Control & Life Support Sect, Noordwijk, Netherlands
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Ushakova, S.A.; Manukovsky, N.S.; Lisovsky, G.M.; Kudenko, Y.A.; Kovalev, V.S.; Gubanov, V.G.; Barkhatov, Y.V.; Gribovskaya, I.V.; Zolotukhin, I.G.; Gros, J.B.; Lasseur, C...; Nelson, M \ed.\; Pechurkin, NS \ed.\; Dempster, WF \ed.\; Somova, LA \ed.\; Somo, , LA \ed.\

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18.

Mass exchange in an experimental new-generation life support system model based on biological regeneration of environment / A. A. Tikhomirov [et al.] // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1711-1720, DOI 10.1016/S0273-1177(03)80017-0 . - ISSN 0273-1177
Кл.слова (ненормированные):
Biomass -- Photosynthesis -- Plants (botany) -- Transpiration -- Mass exchange -- Life support systems (spacecraft) -- ammonia -- nitrogen -- oxygen -- biosphere -- animal -- annelid worm -- article -- biological model -- biomass -- bioremediation -- evaluation -- growth, development and aging -- human -- metabolism -- microclimate -- photosynthesis -- Pleurotus -- radish -- wheat -- Ammonia -- Animals -- Biodegradation, Environmental -- Biomass -- Ecological Systems, Closed -- Evaluation Studies -- Humans -- Life Support Systems -- Models, Biological -- Nitrogen -- Oligochaeta -- Oxygen -- Photosynthesis -- Pleurotus -- Raphanus -- Triticum
Аннотация: An experimental model of a biological life support system was used to evaluate qualitative and quantitative parameters of the internal mass exchange. The photosynthesizing unit included the higher plant component (wheat and radish), and the heterotrophic unit consisted of a soil-like substrate, California worms, mushrooms and microbial microflora. The gas mass exchange involved evolution of oxygen by the photosynthesizing component and its uptake by the heterotroph component along with the formation and maintaining of the SLS structure, growth of mushrooms and California worms, human respiration, and some other processes. Human presence in the system in the form of "virtual human" that at regular intervals took part in the respirative gas exchange during the experiment. Experimental data demonstrated good oxygen/carbon dioxide balance, and the closure of the cycles of these gases was almost complete. The water cycle was nearly 100% closed. The main components in the water mass exchange were transpiration water and the watering solution with mineral elements. Human consumption of the edible plant biomass (grains and roots) was simulated by processing these products by a unique physicochemical method of oxidizing them to inorganic mineral compounds, which were then returned into the system and fully assimilated by the plants. The oxidation was achieved by "wet combustion" of organic biomass, using hydrogen peroxide following a special procedure, which does not require high temperature and pressure. Hydrogen peroxide is produced from the water inside the system. The closure of the cycle was estimated for individual elements and compounds. Stoichiometric proportions are given for the main components included in the experimental model of the system. Approaches to the mathematical modeling of the cycling processes are discussed, using the data of the experimental model. Nitrogen, as a representative of biogenic elements, shows an almost 100% closure of the cycle inside the system. The proposed experimental model of a biological system is discussed as a candidate for potential application in the investigations aimed at creating ecosystems with largely closed cycles of the internal mass exchange. The formation and maintenance of sustainable cycling of vitally important chemical elements and compounds in biological life support systems (BLSS) is an extremely pressing problem. To attain the stable functioning of biological life support systems (BLSS) and to maintain a high degree of closure of material cycles in them, it is essential to understand the character of mass exchange processes and stoichiometric proportions of the initial and synthesized components of the system. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russian Federation
Universite B. Pascal, Clermont-Ferrand, France
Environ. Control/Life Support Sect., ESA, Estec Noorwijk, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Ushakova, S.A.; Manukovsky, N.S.; Lisovsky, G.M.; Kudenko, Yu.A.; Kovalev, V.S.; Gubanov, V.G.; Barkhatov, Yu.V.; Gribovskaya, I.V.; Zolotukhin, I.G.; Gros, J.B.; Lasseur, Ch.

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19.

Information capacity of various triplets in the problem of the evolution of genetic systems [Текст] / M. A. Mamonova, M. G. Sadovsky // Zhurnal Obshchei Biol. - 2003. - Vol. 64, Is. 5. - P. 421-433. - Cited References: 44 . - ISSN 0044-4596

РУБ Biology
Рубрики:
NUCLEOTIDE-SEQUENCES
   CODON FREQUENCIES
   DNA-SEQUENCES
   WORDS
   LINGUISTICS
   PREDICTION
   REDUNDANCY
   INTRONS
   REGIONS
   TEXTS
Аннотация: New method to reveal the sites in genomes obtaining the high information capacity is developed. A distribution of those sites of the length 3 among 16 viral genomes and I I bacteriophages genomes has been studied. It is shown that some tripletes with high information capacity occur in a family of relatively close genomes with the increased frequency. The molecular evolution aspects of a persistence of highly scored sites with respect to their information capacity among various genomes are discussed.

WOS
Держатели документа:
Krasnoyarsk State Univ, Krasnoyarsk 660041, Russia
RAS, SD, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Mamonova, M.A.; Sadovsky, M.G.

Найти похожие

20.

Mass exchange in an experimental new-generation life support system model based on biological regeneration of environment [Text] / A. A. Tikhomirov [et al.] ; ed. M Nelson [et al.] // SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - P1711-1720, DOI 10.1016/S0273-1177(03)00108-X. - Cited References: 13 . - 10. - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Ecology + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences

Аннотация: An experimental model of a biological life support system was used to evaluate qualitative and quantitative parameters of the internal mass exchange. The photosynthesizing unit included the higher plant component (wheat and radish), and the heterotrophic unit consisted of a soil-like substrate, California worms, mushrooms and microbial microflora. The gas mass exchange involved evolution of oxygen by the photosynthesizing component and its uptake by the heterotroph component along with the formation and maintaining of the SLS structure, growth of mushrooms and California worms, human respiration, and some other processes. Human presence in the system in the form of "virtual human" that at regular intervals took part in the respirative gas exchange during the experiment. Experimental data demonstrated good oxygen/carbon dioxide balance, and the closure of the cycles of these gases was almost complete. The water cycle was nearly 100% closed. The main components in the water mass exchange were transpiration water and the watering solution with mineral elements. Human consumption of the edible plant biomass (grains and roots) was simulated by processing these products by a unique physicochemical method of oxidizing them to inorganic mineral compounds, which were then returned into the system and fully assimilated by the plants. The oxidation was achieved by "wet combustion" of organic biomass, using hydrogen peroxide following a special procedure, which does not require high temperature and pressure. Hydrogen peroxide is produced from the water inside the system. The closure of the cycle was estimated for individual elements and compounds. Stoichiometric proportions are given for the main components included in the experimental model of the system. Approaches to the mathematical modeling of the cycling processes are discussed, using the data of the experimental model. Nitrogen, as a representative of biogenic elements, shows an almost 100% closure of the cycle inside the system, The proposed experimental model of a biological system is discussed as a candidate for potential application in the investigations aimed at creating ecosystems with largely closed cycles of the internal mass exchange. The formation and maintenance of sustainable cycling of vitally important chemical elements and compounds in biological life support systems (BLSS) is an extremely pressing problem. To attain the stable functioning of biological life support systems (BLSS) and to maintain a high degree of closure of material cycles in them, it is essential to understand the character of mass exchange processes and stoichiometric proportions of the initial and synthesized components of the system. (C) 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Держатели документа:
Russian Acad Sci, Inst Biophys, Krasnoyarsk, Russia
Univ Strasbourg 1, Clermont Ferrand, France
ESA, Estec, Environm Control & Life Support Sect, Noordwijk, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Ushakova, S.A.; Manukovsky, N.S.; Lisovsky, G.M.; Kudenko, Y.A.; Kovalev, V.S.; Gubanov, V.G.; Barkhatov, Y.V.; Gribovskaya, I.V.; Zolotukhin, I.G.; Gros, J.B.; Lasseur, C...; Nelson, M \ed.\; Pechurkin, NS \ed.\; Dempster, WF \ed.\; Somova, LA \ed.\; Somo, , LA \ed.\

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