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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vysotski E.S., Liu Z.J., Markova S.V., Blinks J.R., Deng L..., Frank L.A., Herko M..., Malikova N.P., Rose J.P., Wang B.C., Lee J...
Заглавие : Violet bioluminescence and fast kinetics from W92F obelin: Structure-based proposals for the bioluminescence triggering and the identification of the emitting species
Колич.характеристики :12 с
Место публикации : Biochemistry: AMER CHEMICAL SOC, 2003. - Vol. 42, Is. 20. - С. 6013-6024. - ISSN 0006-2960, DOI 10.1021/bi027258h
Примечания : Cited References: 45
Предметные рубрики: RAY CRYSTALLOGRAPHIC ANALYSIS
PHOTOPROTEIN AEQUORIN
ANGSTROM RESOLUTION
RECOMBINANT OBELIN
CALCIUM
LUMINESCENCE
LONGISSIMA
EVOLUTION
PROTEINS
COELENTERAZINE
Аннотация: Obelin from the hydroid Obelia longissima and aequorin are members of a subfamily of Ca2+-regulated photoproteins that is a part of the larger EF-hand calcium binding protein family. On the addition of Ca2+, obelin generates a blue bioluminescence emission (lambda(max) = 485 nm) as the result of the oxidative decarboxylation of the bound substrate, coelenterazine. The W92F obelin mutant is noteworthy because of the unusually high speed with which it responds to sudden changes of [Ca2+] and because it emits violet light rather than blue due to a prominent band with lambda(max) = 405 nm. Increase of pH in the range from 5.5 to 8.5 and using D2O both diminish the contribution of the 405 nm band, indicating that excited state proton transfer is involved. Fluorescence model studies have suggested the origin of the 485 nm emission as the excited state of an anion of coelenteramide, the bioluminescence reaction product, and 405 nm from the excited neutral state. Assuming that the dimensions of the substrate binding cavity do not change during the excited state formation, a His22 residue within hydrogen bonding distance to the 6-(p-hydroxy)-phenyl group of the excited coelenteramide is a likely candidate for accepting the phenol proton to produce an ion-pair excited state, in support of recent suggestions for the bioluminescence emitting state. The proton transfer could be impeded by removal of the Trp92 H-bond, resulting in strong enhancement of a 405 nm band giving the violet color of bioluminescence. Comparative analysis of 3D structures of the wild-type (WT) and W92F obelins reveals that there are structural displacements of certain key Ca2+-ligating residues in the loops of the two C-terminal EF hands as well as clear differences in hydrogen bond networks in W92F. For instance, the hydrogen bond between the side-chain oxygen atom of Asp 169 and the main-chain nitrogen of Arg112 binds together the incoming alpha-helix of loop III and the exiting cc-helix of loop IV in WT, providing probably concerted changes in these EF hands on calcium binding. But this linkage is not found in W92F obelin. These differences apparently do not change the overall affinity to calcium of W92F obelin but may account for the kinetic differences between the WT and mutant obelins. From analysis of the hydrogen bond network in the coelenterazine binding cavity, it is proposed that the trigger for bioluminescence reaction in these Ca2+-regulated photoproteins may be a shift of the hydrogen bond donor-acceptor separations around the coelenterazine-2-hydroperoxy substrate, initiated by small spatial adjustment of the exiting a-helix of loop IV.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petrova A. S., Alieva R. R., Belogurova N. V., Tirranen L. S., Kudryasheva N. S.
Заглавие : Variation of Spectral Characteristics of Coelenteramide-Containing Fluorescent Protein from Obelia Longissima Exposed to Dimethyl Sulfoxide
Колич.характеристики :6 с
Коллективы : Russian Science Foundation [14-14-00076]
Место публикации : Russ. Phys. J.: SPRINGER, 2016. - Vol. 59, Is. 4. - С. 562-567. - ISSN 1064-8887, DOI 10.1007/s11182-016-0806-8. - ISSN 1573-9228(eISSN)
Примечания : Cited References:33. - This work was supported in part by the Russian Science Foundation (Contract No. 14-14-00076).
Предметные рубрики: CA2+-REGULATED PHOTOPROTEINS
SPECTROSCOPIC PROPERTIES
Ключевые слова (''Своб.индексиров.''): fluorescent coelenteramide-containing fluorescent proteins--discharged--obelin--proton transfer--dimethyl sulfoxide
Аннотация: Effect of dimethyl sulfoxide (DMSO), a widespread biomedical agent, on spectral-luminescent characteristics of coelenteramide-containing fluorescent protein - discharged obelin - is investigated. Contributions of violet and blue-green spectral components to fluorescence of discharged obelin are elucidated and characterized at different photoexcitation energies. Dependences of these contributions on the DMSO concentration are presented. Spectral changes are related to the destructive effect of DMSO on fluorescent protein and decreasing efficiency of proton transfer to electronically excited states of fluorophore.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Alieva R. R., Kudryasheva N. S.
Заглавие : Variability of fluorescence spectra of coelenteramide-containing proteins as a basis for toxicity monitoring
Место публикации : Talanta: Elsevier B.V., 2017. - Vol. 170. - С. 425-431. - ISSN 00399140 (ISSN) , DOI 10.1016/j.talanta.2017.04.043
Ключевые слова (''Своб.индексиров.''): coelenteramide-containing fluorescent protein--multicolor fluorescent bioassay--obelin--primary photochemical process--protein destruction--proton transfer--bioassay--biomarkers--excited states--fluorescence--fluorophores--ionizing radiation--proton transfer--toxicity--electron-excited state--fluorescence spectra--fluorescent protein--green fluorescent protein--obelin--photochemical process--photochemical properties--physicochemical process--proteins
Аннотация: Nowadays, physicochemical approach to understanding toxic effects remains underdeveloped. A proper development of such mode would be concerned with simplest bioassay systems. Coelenteramide-Containing Fluorescent Proteins (CLM-CFPs) can serve as proper tools for study primary physicochemical processes in organisms under external exposures. CLM-CFPs are products of bioluminescent reactions of marine coelenterates. As opposed to Green Fluorescent Proteins, the CLM-CFPs are not widely applied in biomedical research, and their potential as colored biomarkers is undervalued now. Coelenteramide, fluorophore of CLM-CFPs, is a photochemically active molecule; it acts as a proton donor in its electron-excited states, generating several forms of different fluorescent state energy and, hence, different fluorescence color, from violet to green. Contributions of the forms to the visible fluorescence depend on the coelenteramide microenvironment in proteins. Hence, CLM-CFPs can serve as fluorescence biomarkers with color differentiation to monitor results of destructive biomolecule exposures. The paper reviews experimental and theoretical studies of spectral-luminescent and photochemical properties of CLM-CFPs, as well as their variation under different exposures – chemicals, temperature, and ionizing radiation. Application of CLM-CFPs as toxicity bioassays of a new type is justified. © 2017
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gaevsky N.A., Kolmakov V.I., Anishchenko O.V., Gorbaneva T.B.
Заглавие : Using DCMU-fluorescence method for the identification of dominant phytoplankton groups
Колич.характеристики :12 с
Место публикации : J. Appl. Phycol.: SPRINGER, 2005. - Vol. 17, Is. 6. - P483-494. - ISSN 0921-8971, DOI 10.1007/s10811-005-2903-x
Примечания : Cited References: 28
Предметные рубрики: IN-VIVO
CHLOROPHYLL FLUORESCENCE
FLUOROMETRIC METHOD
SITU
PHOTOSYNTHESIS
CYANOBACTERIA
ALGAE
EVOLUTION
CULTURES
LAKES
Ключевые слова (''Своб.индексиров.''): algae--chlorophyll--fluorescence--vizualization
Аннотация: For the identification of ecologically significant dominant groups of phytoplanktonic algae a polychromatic DCMU-induced fluorescence method is recommended. A special fluorometer equipped with a system of replaceable filters is used to differentiate three regions of the spectrum (410 +/- 20, 510 +/- 20 and 540 +/- 10 nm) that can excite the basic light-harvesting pigments. Total and differential (for every algal taxon studied) chlorophyll a calculated from the fluorescence signals is in good agreement with biomass estimates from direct cell counts for several different trophic types of aquatic systems. This is made possible by the vizualization of the ratios of fluorescence signal values in their own coordinates: first, to decide whether it is necessary to correct linear equations in order to eliminate negative solutions; second, to determine the possibility of nulling the negative solution if a point is situated close to a side of the triangle; and third, to reduce the number of linear algebraic equations to two if the points are situated along one of the triangle sides or to one if the points are gathered at the apex. The polychromatic DCMU-induced fluorescence method can be used for monitoring natural phytoplankton populations to detect changes in their taxonomic structure.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kudryasheva N.S., Nemtseva E.V., Meshalkin Y.P., Sizykh A.G.
Заглавие : Upper electron-excited states in bioluminescence: experimental indication
Колич.характеристики :4 с
Место публикации : Luminescence: JOHN WILEY & SONS LTD, 2001. - Vol. 16, Is. 3. - P243-246. - ISSN 1522-7235, DOI 10.1002/bio.613
Примечания : Cited References: 22
Ключевые слова (''Своб.индексиров.''): bioluminescence--upper electron-excited states--energy transfer
Аннотация: The involvement of upper electron-excited states in bacterial bioluminescence process was studied with excitation energy-accepting molecules. The fluorescent aromatic compounds, anthracene and 1.4-bis(5-phenyloxazol-2-yl)benzene, were chosen. Energies of their lowest excited singlet states are higher than the energy of the analogous state of the bioluminescence emitter; their absorption spectra and bioluminescence do not overlap. Hence, the excitation of these molecules by singlet-singlet energy transfer or by light absorption is excluded. Sensitized fluorescence of these compounds in the bioluminescence systems has been recorded, indicating the activity of upper electron-excited states in the bioluminescent process. Copyright (C) 2001 John Wiley & Sons, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Alieva R. R., Tomilin F. N., Kuzubov A. A., Ovchinnikov S. G., Kudryasheva N. S.
Заглавие : Ultraviolet fluorescence of coelenteramide and coelenteramide-containing fluorescent proteins. Experimental and theoretical study
Место публикации : J. Photochem. Photobiol. B Biol. - 2016. - Vol. 162. - С. 318-323. - ISSN 10111344 (ISSN) , DOI 10.1016/j.jphotobiol.2016.07.004
Ключевые слова (''Своб.индексиров.''): aequorin--b3lyp--coelenteramide--discharged photoproteins--excitation energy--fluorescence--fluorescent protein--obelin
Аннотация: Coelenteramide-containing fluorescent proteins are products of bioluminescent reactions of marine coelenterates. They are called ‘discharged photoproteins’. Their light-induced fluorescence spectra are variable, depending considerably on external conditions. Current work studies a dependence of light-induced fluorescence spectra of discharged photoproteins obelin, aequorin, and clytin on excitation energy. It was demonstrated that photoexcitation to the upper electron-excited states (260–300 nm) of the discharged photoproteins initiates a fluorescence peak in the near UV region, in addition to the blue-green emission. To characterize the UV fluorescence, the light-induced fluorescence spectra of coelenteramide (CLM), fluorophore of the discharged photoproteins, were studied in methanol solution. Similar to photoproteins, the CLM spectra depended on photoexcitation energy; the additional peak (330 nm) in the near UV region was observed in CLM fluorescence at higher excitation energy (260–300 nm). Quantum chemical calculations by time depending method with B3LYP/cc-pVDZ showed that the conjugated pyrazine-phenolic fragment and benzene moiety of CLM molecule are responsible for the additional UV fluorescence peak. Quantum yields of CLM fluorescence in methanol were 0.028 ± 0.005 at 270–340 nm photoexcitation. A conclusion was made that the UV emission of CLM might contribute to the UV fluorescence of the discharged photoproteins. The study develops knowledge on internal energy transfer in biological structures – complexes of proteins with low-weight aromatic molecules. © 2016 Elsevier B.V.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Van Stokkum I.H.M., Gobets B., Van Mourik F., Lee J., Van Grondelle R., Visser A.J.W.G.
Заглавие : Ultrafast fluorescence relaxation spectroscopy of 6,7-dimethyl-(8-ribityl)-lumazine and riboflavin, free and bound to antenna proteins from bioluminescent bacteria
Место публикации : Journal of Physical Chemistry B. - 2003. - Vol. 107, Is. 39. - С. 10934-10939. - ISSN 15206106 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacteria--bioluminescence--chemical relaxation--chromophores--dielectric properties--proteins--solvents--bioluminescent bacteria--dimethyl ribityl lumazine--photobacterium leiognathi--riboflavin--ultrafast fluorescence relaxation spectroscopy--fluorescence
Аннотация: The solvation dynamics of interesting bioluminescent chromophores have been determined, using subpicosecond and wavelength-resolved fluorescence spectroscopy, in combination with global analysis of the multidimensional data sets. The systems investigated comprise the free ligands 6,7-dimethyl-(8-ribityl)-lumazine (lumazine) and riboflavin in an aqueous buffer and both ligands when noncovalently bound to two bacterial bioluminescent antenna proteins: lumazine protein (from Photobacterium leiognathi) and the blue fluorescent protein (from Vibrio fischeri Y1). Fluorescence spectral relaxation of the free ligands is complete within a few picoseconds. Subsequently, the fluorescence intensity increases by ?7% on a time scale of 15-30 ps. Fluorescence spectral relaxation of the protein-bound ligands is largely complete within 1 ps but reveals a small red shift with a minor, but distinctly longer, relaxation time than that of the free ligands, which is tentatively assigned to the relaxation of protein-bound water in the vicinity of the excited chromophore.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., van Stokkum IHM, Gobets B..., van Mourik F..., Lee J..., van Grondelle R..., Visser AJWG
Заглавие : Ultrafast fluorescence relaxation spectroscopy of 6,7-dimethyl-(8-ribityl)-lumazine and riboflavin, free and bound to antenna proteins from bioluminescent bacteria
Колич.характеристики :6 с
Место публикации : J. Phys. Chem. B: AMER CHEMICAL SOC, 2003. - Vol. 107, Is. 39. - P10934-10939. - ISSN 1520-6106, DOI 10.1021/jp034266e
Примечания : Cited References: 52
Предметные рубрики: TIME-RESOLVED FLUORESCENCE
VIBRIO-FISCHERI Y1
FEMTOSECOND SOLVATION DYNAMICS
FLAVIN ADENINE-DINUCLEOTIDE
PHOTOBACTERIUM-LEIOGNATHI
BIOLOGICAL WATER
SOLVENT DYNAMICS
DIELECTRIC-RELAXATION
MOLECULAR-DYNAMICS
TRYPTOPHAN
Аннотация: The solvation dynamics of interesting bioluminescent chromophores have been determined, using subpicosecond and wavelength-resolved fluorescence spectroscopy, in combination with global analysis of the multidimensional data sets. The systems investigated comprise the free ligands 6,7-dimethyl-(8-ribityl)-lumazine (lumazine) and riboflavin in an aqueous buffer and both ligands when noncovalently bound to two bacterial bioluminescent antenna proteins: lumazine protein (from Photobacterium leiognathi) and the blue fluorescent protein (from Vibrio fischeri Y1). Fluorescence spectral relaxation of the free ligands is complete within a few picoseconds. Subsequently, the fluorescence intensity increases by similar to7% on a time scale of 15-30 ps. Fluorescence spectral relaxation of the protein-bound ligands is largely complete within 1 ps but reveals a small red shift with a minor, but distinctly longer, relaxation time than that of the free ligands, which is tentatively assigned to the relaxation of protein-bound water in the vicinity of the excited chromophore.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Larionova, Marina D., Markova, Svetlana V., Vysotski, Eugene S.
Заглавие : Tyr72 and Tyr80 are Involved in the Formation of an Active Site of a Luciferase of Copepod Metridia longa
Колич.характеристики :8 с
Коллективы : Russian Science Foundation [14-14-01119]
Место публикации : Photochem. Photobiol.: WILEY, 2017. - Vol. 93, Is. 2. - С. 503-510. - ISSN 0031-8655, DOI 10.1111/php.12694. - ISSN 1751-1097(eISSN)
Примечания : Cited References:41. - This work was supported by the grant 14-14-01119 of the Russian Science Foundation.
Предметные рубрики: CA2+-REGULATED PHOTOPROTEIN OBELIN
COELENTERAZINE-BINDING PROTEIN
Аннотация: Luciferase of copepod Metridia longa (MLuc) is a naturally secreted enzyme catalyzing the oxidative decarboxylation of coelenterazine with the emission of light. To date, three nonallelic isoforms of different lengths (17-24 kDa) for M. longa luciferase have been cloned. All the isoforms are single-chain proteins consisting of a 17-residue signal peptide for secretion, variable N-terminal part and conservative C-terminus responsible for luciferase activity. In contrast to other bioluminescent proteins containing a lot of aromatic residues which are frequently involved in light emission reaction, the C-terminal part of MLuc contains only four Phe, two Tyr, one Trp and two His residues. To figure out whether Tyr residues influence bioluminescence, we constructed the mutants with substitution of Tyr to Phe (Y72F and Y80F). Tyrosine substitutions do not eliminate the ability of luciferase to bioluminescence albeit significantly reduce relative specific activity and change bioluminescence kinetics. In addition, the Tyr replacements have no effect on bioluminescence spectrum, thereby indicating that tyrosines are not involved in the emitter formation. However, as it was found that the intrinsic fluorescence caused by Tyr residues is quenched by a reaction substrate, coelenterazine, in concentration-dependent manner, we infer that both tyrosine residues are located in the luciferase substrate-binding cavity.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Darin A. V., Rogozin D. Y., Meydus A. V., Babich V. V., Kalugin I. A., Markovich T. I., Rakshun Y. V., Darin F. A., Sorokoletov D. S., Gogin A. A., Senin R. A., Degermendzhi A. G.
Заглавие : Traces of the Tunguska Event (1908) in Sediments of Zapovednoe Lake Based on SR–XRF Data
Место публикации : Dokl. Earth Sci.: Pleiades Publishing, 2020. - Vol. 492, Is. 2. - С. 442-445. - ISSN 1028334X (ISSN), DOI 10.1134/S1028334X20060045
Аннотация: Abstract: An anomalous layer enriched with chemical elements indicating the presence of terrigenous matter was discovered in the sediment core of Zapovednoe Lake located 60 km from the epicenter of the Tunguska event (1908) using synchrotron radiation X-ray fluorescence spectroscopy (SR–XRF). Radioisotope measurements indicate that the age of the layer is consistent with the date of the catastrophe. Apparently, the anomalous layer was formed as a result of an intense terrigenous matter inflow from the water catchment area due to massive forest falls and subsequent wildfires caused by the Tunguska event. Thus, it is established that targeted searches for microparticles of extraterrestrial origin can be carried out in the discovered and dated anomalous bottom sediment layer. © 2020, Pleiades Publishing, Ltd.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Darin, A., V, Rogozin, D. Yu, Meydus, A., V, Babich V. V., Kalugin I. A., Markovich, T., I, Rakshun, Ya, V, Darin F. A., Sorokoletov D. S., Gogin A. A., Senin R. A., Degermendzhi A. G.
Заглавие : Traces of the Tunguska Event (1908) in Sediments of Zapovednoe Lake Based on SR-XRF Data
Колич.характеристики :4 с
Коллективы : Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [19-04-00320, 19-05-50046]; Institute of Nuclear Physics, Siberian Branch, Russian Academy of SciencesRussian Academy of Sciences [RFMEFI62119X0022]
Место публикации : Dokl. Earth Sci.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2020. - Vol. 492, Is. 2. - С. 442-445. - ISSN 1028-334X, DOI 10.1134/S1028334X20060045. - ISSN 1531-8354(eISSN)
Примечания : Cited References:10. - This study was performed as a part of a State Assignment of the Institute of Geology and Mineralogy, Siberian Branch, Russian Academy of Sciences, and supported by the Russian Foundation for Basic Research, project nos. 19-04-00320 and 19-05-50046. This study was per-formed in the Shared Research Center "Siberian Synchrotron and Terahertz Radiation Center" on the basis of the VEPP-4-VEPP-2000 Electron-Positron Collider Complex of the Institute of Nuclear Physics, Siberian Branch, Russian Academy of Sciences, using equipment supported by project no. RFMEFI62119X0022.
Аннотация: An anomalous layer enriched with chemical elements indicating the presence of terrigenous matter was discovered in the sediment core of Zapovednoe Lake located 60 km from the epicenter of the Tunguska event (1908) using synchrotron radiation X-ray fluorescence spectroscopy (SR-XRF). Radioisotope measurements indicate that the age of the layer is consistent with the date of the catastrophe. Apparently, the anomalous layer was formed as a result of an intense terrigenous matter inflow from the water catchment area due to massive forest falls and subsequent wildfires caused by the Tunguska event. Thus, it is established that targeted searches for microparticles of extraterrestrial origin can be carried out in the discovered and dated anomalous bottom sediment layer.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ushakova S.A., Tikhomirov A.A., Shikhov V.N., Gros J.-B., Golovko T.K., Dal'Ke I.V., Zakhozhii I.G.
Заглавие : Tolerance of wheat and lettuce plants grown on human mineralized waste to high temperature stress
Место публикации : Advances in Space Research. - 2013. - Vol. 51, Is. 11. - С. 2075-2083. - ISSN 02731177 (ISSN) , DOI 10.1016/j.asr.2013.01.017
Ключевые слова (''Своб.индексиров.''): air temperature step--blss--heat resistance--human waste recycling--lettuce--wheat--air temperature--blss--human waste--lettuce--wheat--atmospheric temperature--electromagnetic field effects--electromagnetic fields--gas plants--heat resistance--oxygen supply--plants (botany)--productivity--specific heat--thermal stress--waste utilization--plant shutdowns
Аннотация: The main objective of a life support system for space missions is to supply a crew with food, water and oxygen, and to eliminate their wastes. The ultimate goal is to achieve the highest degree of closure of the system using controlled processes offering a high level of reliability and flexibility. Enhancement of closure of a biological life support system (BLSS) that includes plants relies on increased regeneration of plant waste, and utilization of solid and liquid human wastes. Clearly, the robustness of a BLSS subjected to stress will be substantially determined by the robustness of the plant components of the phototrophic unit. The aim of the present work was to estimate the heat resistance of two plants (wheat and lettuce) grown on human wastes. Human exometabolites mineralized by hydrogen peroxide in an electromagnetic field were used to make a nutrient solution for the plants. We looked for a possible increase in the heat tolerance of the wheat plants using changes in photosynthetically active radiation (PAR) intensity during heat stress. At age 15 days, plants were subjected to a rise in air temperature (from 23 В± 1 В°C to 44 В± 1 В°) under different PAR intensities for 4 h. The status of the photosynthetic apparatus of the plants was assessed by external 2 gas exchange and fluorescence measurements. The increased irradiance of the plants during the high temperature period demonstrated its protective action for both the photosynthetic apparatus of the leaves and subsequent plant growth and development. The productivity of the plants subjected to temperature changes at 250 W m-2 of PAR did not differ from that of controls, whereas the productivity of the plants subjected to the same heat stress but in darkness was halved. В© 2012 COSPAR. Published by Elsevier Ltd.
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13.

Вид документа : Статья из сборника (выпуск продолж. издания)
Шифр издания :
Автор(ы) : Tikhomirov A.A., Ushakova S.A., Shikhov V.N., Gros J.-B., Golovko T.K., Dal'Ke I.V., Zakhozhii I.G.
Заглавие : Tolerance of plants grown on human mineralized waste to changes in air temperature
Место публикации : International Astronautical Federation - 59th International Astronautical Congress 2008, IAC 2008. - 2008. - Vol. 1: 59th International Astronautical Congress 2008, IAC 2008 (29 September 2008 through 3 October 2008, Glasgow) Conference code: 79748. - С. 311-320
Ключевые слова (''Своб.индексиров.''): air temperature--alternating electromagnetic field--biological life support systems--closedness--controlled process--fluorescence measurements--gas exchange--heat stress--high temperature--human waste--nutrient solution--old plants--photosynthetic apparatus--photosynthetically active radiation--plant components--plant growth--plant wastes--protective action--reproductive organs--temperature changes--temperature conditions--atmospheric temperature--electromagnetic field effects--electromagnetic fields--gas plants--heat resistance--hydrogen peroxide--oxygen supply--plant life extension--productivity--specific heat--thermal stress--waste utilization--plant shutdowns
Аннотация: The main objective of an LSS is to supply a crew with food, water and oxygen, and to eliminate its waste. The ultimate goal is to achieve the highest degree of closure of the system using controlled processes offering a high level of reliability and flexibility. Enhancement of closedness of biological life support systems (BLSS) including plants relies on increased regeneration of gas, water and plant waste, and utilization of solid and liquid human wastes. Clearly, the robustness of an LSS subjected to stress will be substantially determined by the robustness of the plant components of the phototrophic unit. The aim of the present work was to estimate the heat resistance of plants grown on human wastes. Human exometabolites mineralized by hydrogen peroxide in an alternating electromagnetic field were used to make a nutrient solution for the plants. We looked for a possible increase in the heat resistance of the plants using changes in photosynthetically active radiation (PAR) intensity during heat stress. At ages 15 and 25 days, plants were subjected to a rise in air temperature (from 22-24В°C to 44В°C) under different PAR intensities for 4 hours. The status of the photosynthetic apparatus of the plants was assessed by external CO2 gas exchange and fluorescence measurements. The increased irradiance of the plants during the high temperature period demonstrated its protective action for both the photosynthetic apparatus of the leaves and subsequent plant growth and development. The productivity of the plants subjected to temperature changes at 250 W/m2 of PAR did not differ from that of controls, whereas the productivity of the plants subjected to the same heat stress but in darkness was halved. The heat resistance of the reproductive organs of 25-day-old plants was significantly lower than that of 15-day-old plants subjected to similar light and temperature conditions.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nesterenko T.V., Shikhov V.N., Tikhomirov A.A.
Заглавие : Thermoinduction of chlorophyll fluorescence and the age-related condition of higher plant leaves
Колич.характеристики :8 с
Место публикации : Russ. J. Plant Physiol.: MAIK NAUKA/INTERPERIODICA, 2001. - Vol. 48, Is. 2. - P244-251. - ISSN 1021-4437, DOI 10.1023/A:1009016520582
Примечания : Cited References: 27
Ключевые слова (''Своб.индексиров.''): thermoinduction of chlorophyll fluorescence--leaf--development
Аннотация: The age-related changes in the temperature dependence curves (TDC) of chlorophyll fluorescence were studied in leaf segments of wheat (Triticum aestivum L.), tomato (Lycopersicum esculentum Mill.), and cucumber (Cucumis sativum L.) plants grown under controlled photoculture conditions. Three major TDC patterns of chlorophyll fluorescence were identified within the temperature range of 25-70 degreesC, with each of the patterns corresponding to a certain phase of leaf development. The transition from one type of thermogram to another was a gradual and ordered process. The magnitude of the low-temperature TDC peak increased until leaves completely expanded and declined with leaf senescence. In the course of leaf senescence, the thermograms exhibited an additional shoulder, which further changed into a peak at 55-65 degreesC with increasing magnitude. Our data provide the basis for assessing leaf age from the type of chlorophyll fluorescence thermogram and the changes in the particular indices characteristic of TDC of chlorophyll fluorescence.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nesterenko T.V., Shikhov V.N., Tikhomiro A.A.
Заглавие : Thermoinduction of chlorophyll fluorescence and the age-related condition of higher plant leaves
Место публикации : Russian Journal of Plant Physiology. - 2001. - Vol. 48, Is. 2. - С. 244-251. - ISSN 10214437 (ISSN) , DOI 10.1023/A:1009016520582
Ключевые слова (''Своб.индексиров.''): development--leaf--thermoinduction of chlorophyll fluorescence--cucumis--cucumis sativus--embryophyta--lycopersicon esculentum--sativum--triticum aestivum
Аннотация: The age-related changes in the temperature dependence curves (TDC) of chlorophyll fluorescence were studied in leaf segments of wheat (Triticum aestivum L.), tomato (Lycopersicum esculentum Mill.), and cucumber (Cucumis sativum L.) plants grown under controlled photoculture conditions. Three major TDC patterns of chlorophyll fluorescence were identified within the temperature range of 25-70В°C, with each of the patterns corresponding to a certain phase of leaf development. The transition from one type of thermogram to another was a gradual and ordered process. The magnitude of the low-temperature TDC peak increased until leaves completely expanded and declined with leaf senescence. In the course of leaf senescence, the thermograms exhibited an additional shoulder, which further changed into a peak at 55-65В°C with increasing magnitude. Our data provide the basis for assessing leaf age from the type of chlorophyll fluorescence thermogram and the changes in the particular indices characteristic of TDC of chlorophyll fluorescence.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Gibson B.G., Lee J.
Заглавие : The yellow bioluminescence bacterium, Vibrio fischeri Y1, contains a bioluminescence active riboflavin protein in addition to the yellow fluorescence FMN protein
Место публикации : Biochemical and Biophysical Research Communications. - 1995. - Vol. 211, Is. 3. - С. 774-779. - ISSN 0006291X (ISSN) , DOI 10.1006/bbrc.1995.1880
Ключевые слова (''Своб.индексиров.''): riboflavin--article--bioluminescence--fluorescence--nonhuman--priority journal--protein analysis--protein synthesis--vibrio--vibrionaceae--bacterial proteins--chromatography, gel--chromatography, thin layer--flavin mononucleotide--flavoproteins--luminescence--riboflavin--spectrometry, fluorescence--support, u.s. gov't, p.h.s.--vibrio--bacteria (microorganisms)--photobacterium--vibrio--vibrio fischeri
Аннотация: The yellow bioluminescence Y1 strain of Vibrio fischeri can produce a 22 kDa protein with either FMN or riboflavin as a bound fluorophore. Both forms are active for shifting the bioluminescence spectral maximum. The fluorescence spectral distribution of the two proteins differs slightly and the in vivo emission appears to be an equal mixture of the two. The bioluminescence activity of the riboflavin Y1 protein contrasts with the inactivity of the related Photobacterium type.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : KUDRYAVTSEVA O.A., BARTSEV S.I., OKHONIN V.A., MEZHEVIKIN V.V.
Заглавие : THE LOCALIZATION OF LUMINESCENT SYSTEM OF LUMINOUS BACTERIA
Колич.характеристики :5 с
Место публикации : Biofizika: MEZHDUNARODNAYA KNIGA, 1993. - Vol. 38, Is. 3. - P435-439. - ISSN 0006-3029
Примечания : Cited References: 10
Аннотация: A method for localization of a light source near the interface of two media is described. The method is based on an optical analog of tunnel effect when the radiation source is at the distance smaller than the wavelength from the interface. Application of the tunnel effect permits to obtain high resolution. The developed method was used to determine the localization of a bacterial luminescent system. It has been found that the sources of bioluminescence are located at thin subsurfase lager with width about 70 nm. This result is in favour of the peripheral (periplasmatic or membrane) localization of the bacterial luminescent system. This method makes it possible to investigate processes pelated to light radiation (luminescence, fluorescence and other optical processes) in a thin surface layer of various biological and physical objects.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Markova S.V., Burakova L.P., Golz S..., Malikova N.P., Frank L.A., Vysotski E.S.
Заглавие : The light-sensitive photoprotein berovin from the bioluminescent ctenophore Beroe abyssicola: a novel type of Ca2+-regulated photoprotein
Колич.характеристики :15 с
Место публикации : FEBS J.: WILEY-BLACKWELL, 2012. - Vol. 279, Is. 5. - С. 856-870. - ISSN 1742-464X, DOI 10.1111/j.1742-4658.2012.08476.x
Примечания : Cited References: 63. - The authors thank Natalia Chervyakova from Department of Zoology of Invertebrates of Moscow State University for the photo of the White Sea ctenophore Beroe abyssicola. This work was supported by RFBR grant 09-04-00172, by grant 64987.2010.4, Molecular and Cellular Biology program of RAS, and Bayer Pharma AG (Germany).
Предметные рубрики: CALCIUM-ACTIVATED PHOTOPROTEINS
C-TERMINAL PROLINE
SEQUENCE-ANALYSIS
MNEMIOPSIS-SP
COELENTERAZINE-BINDING
ANGSTROM RESOLUTION
RECOMBINANT OBELIN
CRYSTAL-STRUCTURES
EXCITED-STATE
CDNA CLONING
Ключевые слова (''Своб.индексиров.''): bioluminescence--calcium--coelenterazine--luciferase--mammalian expression
Аннотация: Light-sensitive Ca2+-regulated photoproteins are responsible for the bright bioluminescence of ctenophores. Using functional screening, four full-size cDNA genes encoding the same 208-amino-acid polypeptide were isolated from two independent cDNA libraries prepared from two Beroe abyssicola specimens. Sequence analysis revealed three canonical EF-hand calcium-binding sites characteristic of Ca2+-regulated photoproteins, but a very low degree of sequence identity (2729%) with aequorin-type photoproteins, despite functional similarities. Recombinant berovin was expressed in Escherichia coli cells, purified, converted to active photoprotein and characterized. Active berovin has absorption maxima at 280 and 437 nm. The Ca2+-discharged protein loses visible absorption, but exhibits a new absorption maximum at 335 nm. The berovin bioluminescence is blue (?max = 491 nm) and a change in pH over the range 6.09.5 has no significant effect on the light emission spectrum. By contrast, the fluorescence of Ca2+-discharged protein (?ex = 350 nm) is pH sensitive: at neutral pH the maximum is at 420 nm and at alkaline pH there are two maxima at 410 and 485 nm. Like native ctenophore photoproteins, recombinant berovin is also inactivated by light. The Ca2+ concentrationeffect curve is a sigmoid with a slope on a loglog plot of similar to 2.5. Although this curve for berovin is very similar to those obtained for obelin and aequorin, there are evident distinctions: berovin responds to calcium changes at lower concentrations than jellyfish photoproteins and its Ca2+-independent luminescence is low. Recombinant berovin was successfully expressed in mammalian cells, thereby demonstrating potential for monitoring intracellular calcium.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Eremeeva E.V., Markova S.V., Westphal A.H., Visser AJWG, van Berkel WJH, Vysotski E.S.
Заглавие : The intrinsic fluorescence of apo-obelin and apo-aequorin and use of its quenching to characterize coelenterazine binding
Колич.характеристики :6 с
Коллективы : Wageningen University Sandwich PhD-Fellowship Program [02.512.12. 2006]; Ministry of Education and Science of Russian Federation, MCB Program of RAS [1211.2008.4]; SB RAS
Место публикации : FEBS Lett.: ELSEVIER SCIENCE BV, 2009. - Vol. 583, Is. 12. - С. 1939-1944. - ISSN 0014-5793, DOI 10.1016/j.febslet.2009.04.043
Примечания : Cited References: 28. - We thank Prof. John Lee for valuable suggestions and providing constructive criticisms. The work was supported by Wageningen University Sandwich PhD-Fellowship Program, Grants 02.512.12. 2006 and 1211.2008.4 of Ministry of Education and Science of Russian Federation, MCB Program of RAS, and by Grant No. 2 of SB RAS.
Предметные рубрики: CRYSTAL-STRUCTURE
CA2+-REGULATED PHOTOPROTEINS
VIOLET BIOLUMINESCENCE
ANGSTROM RESOLUTION
RECOMBINANT OBELIN
W92F OBELIN
CALCIUM
REGENERATION
APOAEQUORIN
EXPRESSION
Ключевые слова (''Своб.индексиров.''): bioluminescence--photoprotein--trp fluorescence
Аннотация: The intrinsic fluorescence of two apo-photoproteins has been characterized and its concentration-dependent quenching by coelenterazine has been for the first time applied to determine the apparent dissociation constants for coelenterazine binding with apo-aequorin (1.2 +/- 0.12 mu M) and apo-obelin (0.2 +/- 0.04 mu M). Stopped-flow measurements of fluorescence quenching showed that coelenterazine binding is a millisecond-scale process, in contrast to the formation of an active photoprotein complex taking several hours. This finding evidently shows that the rate-limiting step of active photoprotein formation is the conversion of coelenterazine into its 2-hydroperoxy derivative. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nesterenko T. V., Shikhov V. N., Tikhomirov A. A.
Заглавие : The fluorescence method for determining of photosynthetic apparatus reactivity in plant leaves
Колич.характеристики :13 с
Место публикации : Zhurnal Obshchei Biol.: MEZHDUNARODNAYA KNIGA, 2019. - Vol. 80, Is. 3. - С. 187-199. - ISSN 0044-4596, DOI 10.1134/S0044459619030060
Примечания : Cited References:31
Предметные рубрики: ELECTRIC-POTENTIAL DIFFERENCE
FREQUENTLY ASKED QUESTIONS
CHLOROPHYLL
Аннотация: Presently, the most promising way of studying, forecasting, and enhancing of organisms' tolerance to harsh environmental impacts is considered to be the estimation of initial functional state of an organism's regulatory systems. To resolve the problem of photosynthetic apparatus (PSA) tolerance to harsh impacts at the level of such a complicated functional system as a plant leaf, it is necessary to assess integral responses of the leaf's PSA to the impact. At that, simple and versatile traits may have certain advantages. At present, chlorophyll fluorescence seems to be one of the main indices of PSA activity, which can be measured relatively fast and easy. One of the possible approaches to operational integrative assessment of PSA activity may consist in usage of the parameters introduced for the curves of chlorophyll fluorescence induction (CFI) slow phase. Temporal patterns of CFI are of special interest. The simplest index T-0.5 (i.e., half-time of chlorophyll fluorescence intensity decrease during the slow phase of CFI) appears to be an integral characteristic of activation rate with regard to a number of photo-assimilation and photo-protective processes in leaves. On basis of the studies, conducted earlier, and published data, we have analyzed the behavior of T-0.5 parameter with comparison to other CFI traits (namely: qN - non-photochemical quenching coefficient, ETR - electron transport rate, Phi(PSII) - effective quantum yield of PSII photochemistry, F-p/F-T ratio) under different conditions. The influence of leaf senescence, changes in intensity of excitation light, slight dehydration of plants and their recovery from water deficiency have been examined. The pattern of T-0.5 behavior, observed in laboratory experiments, and the results of its comparison with other indices of CFI give occasion to propose the usage of T-0.5 for indirect estimation of PSA activity when operational integrative monitoring of PSA state is required. Further studies are necessary for establishing quantitative relationships between PSA activity and fluorescence parameter T-0.5 under specific stress conditions.
WOS,
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