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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolmakov V.I., Levin L.A., Dubovskaya O.P., Gladyshev M.I.
Заглавие : A circadian rhythm of grazing of microalgae by a laboratory culture of Ceriodaphnia quadrangula
Колич.характеристики :4 с
Место публикации : Biofizika: MEZHDUNARODNAYA KNIGA, 2002. - Vol. 47, Is. 4. - P673-676. - ISSN 0006-3029
Примечания : Cited References: 12
Предметные рубрики: CLOSED CULTIVATORS
FLUORESCENCE
ZOOPLANKTON
Ключевые слова (''Своб.индексиров.''): daily rhythm of grazing--fluorescence--microalgae--flowing-through cultivator--photoperiod--statistical spectral analysis
Аннотация: The circadian rhythm of grazing of microalgae by a laboratory culture of Ceriodaphnia quadrangula under continuous illumination was studied by continuous registration of chlorophyll fluorescence at the outlet of a flow-through cultivator. A culture of green alga Chlorella vulgaris was used as a feed. The data obtained were treated by the statistical spectral analysis. It was found that animals preliminarily grown under a 12 h light : 12 h dark regime and transferred to constant light showed two maxima in the circadian rhythm of grazing with periods of 0.7 and 1.1 h. Animals preliminarily grown under constant light showed no circadian rhythm of grazing. It was concluded that the circadian rhythm of grazing of C quadrangula has endogenous nature and can change according to light conditions.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolmakov V.I., Levin L.A., Dubovskaya O.P., Gladyshev M.I.
Заглавие : A circadian rhythm of grazing of microalgae by a laboratory cultureof Ceriodaphnia quadrangula
Место публикации : Biofizika. - 2002. - Vol. 47, Is. 4. - С. 676. - ISSN 00063029 (ISSN)
Ключевые слова (''Своб.индексиров.''): daily rhythm of grazing--flowing-through cultivator--fluorescence--microalgae--photoperiod--statistical spectral analysis--algae--animalia--ceriodaphnia quadrangula--chlorella vulgaris--chlorophyta
Аннотация: The circadian rhythm of grazing of microalgae by a laboratory culture of Ceriodaphnia quadrangula under continuous illumination was studied by continuous registration of chlorophyll fluorescence at the outlet of a flow-through cultivator. A culture of green alga Chlorella vulgaris was used as a feed. The data obtained were treated by the statistical spectral analysis. It was found that animals preliminarily grown under a 12 h light : 12 h dark regime and transferred to constant light showed two maxima in the circadian rhythm of grazing with periods of 0.7 and 1.1 h. Animals preliminarily grown under constant light showed no circadian rhythm of grazing. It was concluded that the circadian rhythm of grazing of C. quadrangula has endogenous nature and can change according to light conditions.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Dubinnyi M.A., Tsarkova A.S., Rodionova N.S., Baranov M.S., Kublitski V.S., Shimomura O..., Yampolsky I.V.
Заглавие : A Novel Type of Luciferin from the Siberian Luminous Earthworm Fridericia heliota: Structure Elucidation by Spectral Studies and Total Synthesis
Колич.характеристики :3 с
Коллективы : Government of the Russian Federation "Measures to attract leading scientists to Russian educational institutions" [11. G34.31.0058]; programs MCB RAS; Russian Federation "Leading science school" [3951.2012.4]; Russian Foundation for Basic Research [14-03-01015]; Russian Federation
Место публикации : Angew. Chem.-Int. Edit.: WILEY-V C H VERLAG GMBH, 2014. - Vol. 53, Is. 22. - С. 5566-5568. - ISSN 1433-7851, DOI 10.1002/anie.201400529. - ISSN 1521-3773
Примечания : Cited References: 13. - We thank Dr. Alexander O. Chizhov for recording mass spectra and Dr. K. S. Mineev for NMR analysis of synthetic intermediates. We acknowledge support from the Program of the Government of the Russian Federation "Measures to attract leading scientists to Russian educational institutions" (grant no. 11. G34.31.0058), the programs MCB RAS, President of the Russian Federation "Leading science school" (grant 3951.2012.4) and the Russian Foundation for Basic Research (grant 14-03-01015). B. M. S. was supported by a stipend from the Program of the President of the Russian Federation.
Предметные рубрики: BIOLUMINESCENT EARTHWORM
Ключевые слова (''Своб.индексиров.''): bioluminescence--luciferin--natural products--nmr spectroscopy--total synthesis
Аннотация: The structure elucidation and synthesis of the luciferin from the recently discovered luminous earthworm Fridericia heliota is reported. This luciferin is a key component of a novel ATP-dependent bioluminescence system. UV, fluorescence, NMR, and HRMS spectroscopy studies were performed on 0.005 mg of the isolated substance and revealed four isomeric structures that conform to spectral data. These isomers were chemically synthesized and one of them was found to produce light when reacted with a protein extract from F. heliota. The novel luciferin was found to have an unusual extensively modified peptidic nature, thus implying an unprecedented mechanism of action.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Antipina L.Y., Tomilin F.N., Vysotskii E.S., Ovchinnikov S.G.
Заглавие : A QUANTUM CHEMICAL STUDY OF THE FORMATION OF 2-HYDROPEROXY-COELENTERAZINE IN THE Ca2+-REGULATED PHOTOPROTEIN OBELIN
Колич.характеристики :6 с
Место публикации : J. Struct. Chem.: SPRINGER, 2011. - Vol. 52, Is. 5. - С. 870-875. - ISSN 0022-4766
Примечания : Cited References: 19. - The work was supported by RFBR (07-04-00930-a), the "Molecular and Cell Biology" Program of the Presidium of the Russian Academy of Sciences, and the Program of the Siberian Division of the Russian Academy of Sciences (project No. 2) within the implementation of the Federal Targeted Program "Scientific and Scientific Pedagogical Personnel of Innovative Russia, 2010" (P333 and P213).
Предметные рубрики: CALCIUM-DISCHARGED OBELIN
SEMIEMPIRICAL METHODS
1.7 ANGSTROM
OPTIMIZATION
PARAMETERS
MECHANISM
FLUORESCENCE
ELEMENTS
PROTEIN
EMITTER
Ключевые слова (''Своб.индексиров.''): coelenterazine--2-hydroperoxy-coelenterazine--obelia longissima--renilla muelleri
Аннотация: The Ca2+-regulated photoprotein obelin determines the luminescence of the marine hydroid Obelia longissima. Bioluminescence is initiated by calcium and appears as a result of the oxidative decarboxylation related to the coelenterazine substrate. The luciferase of the luminescent marine coral Renilla muelleri (RM) also uses coelenterazine as a substrate. However, three proteins are involved in the in vivo bioluminescence of these animals: luciferase, green fluorescent protein, and Ca2+-regulated coelenterazine-binding protein (CBP). In fact, CBP that contains one strongly bound coelenterazine molecule is the RM luciferase substrate in the in vivo bioluminescent reaction. Coelenterazine becomes available for oxygen and the reaction with luciferase only after binding CBP with calcium ions. Unlike Ca2+-regulated photoproteins, the coelenterazine molecule is not activated by oxygen in the CBP molecule. In this work, by means of quantum chemical methods the behavior of substrates in these proteins is analyzed. It is shown that coelenterazine can form different tautomers: CLZ(2H) and CLZ(7H). The formation of 2-hydroperoxy-coelenterazine is studied. According to the obtained data, these proteins use different forms of the substrates for the reaction. In obelin, the substrate is in the CLZ(2H) form that affords hydrogen peroxide. In RM, coelenterazine is in the CLZ(7H) form, and therefore, CBP is not activated by oxygen.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : NESTERENKO T.V., SIDKO F.Y.
Заглавие : AGE-CHANGES OF SLOW FLUORESCENCE INDUCTION IN WHEAT LEAF CHLOROPHYLL
Колич.характеристики :7 с
Место публикации : SOVIET PLANT PHYSIOLOGY: PLENUM PUBL CORP, 1985. - Vol. 32, Is. 3. - P344-350. - ISSN 0038-5719
Примечания : Cited References: 27
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nesterenko T.V., Tikhomirov A.A.
Заглавие : An ontogenetic approach to the assessment of plant resistance to stress factors based on the method of chlorophyll fluorescence induction.
Место публикации : Doklady. Biochemistry and biophysics. - 2003. - Vol. 388. - С. 4-7. - ISSN 16076729 (ISSN)
Ключевые слова (''Своб.индексиров.''): chlorophyll--adaptation--aging--article--comparative study--cucumber--light--metabolism--methodology--photostimulation--photosynthesis--physiology--plant leaf--radiation dose--radiation exposure--spectrofluorometry--adaptation, physiological--aging--chlorophyll--cucumis sativus--light--photic stimulation--photosynthetic reaction center complex proteins--plant leaves--radiation dosage--spectrometry, fluorescence
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nesterenko T.V., Tikhomirov A.A., Shikhov V.N., Zavorueva E.N.
Заглавие : Assessment of plant leaf response to long-term exposure at high temperature using the method of thermoinduced chlorophyll fluorescence.
Место публикации : Doklady. Biochemistry and biophysics. - 2001. - Vol. 378. - С. 165-169. - ISSN 16076729 (ISSN)
Ключевые слова (''Своб.индексиров.''): chlorophyll--pigment--article--biosynthesis--chemistry--light--metabolism--plant leaf--plant physiology--radiation exposure--spectrofluorometry--temperature--thermodynamics--time--chlorophyll--light--pigments, biological--plant leaves--plant physiology--spectrometry, fluorescence--temperature--thermodynamics--time factors
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nemtseva, Elena, V, Gulnov, Dmitry, V, Gerasimova, Marina A., Sukovatyi, Lev A., Burakova, Ludmila P., Karuzina, Natalya E., Melnik, Bogdan S., Kratasyuk, Valentina A.
Заглавие : Bacterial Luciferases from Vibrio harveyi and Photobacterium leiognathi Demonstrate Different Conformational Stability as Detected by Time-Resolved Fluorescence Spectroscopy
Колич.характеристики :17 с
Коллективы : Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]; RFBRRussian Foundation for Basic Research (RFBR) [20-34-90118]; Krasnoyarsk Regional Fund of Science [20-44-243002, 20-44-240006]; RFBRRussian Foundation for Basic Research (RFBR)
Место публикации : Int. J. Mol. Sci.: MDPI, 2021. - Vol. 22, Is. 19. - Ст.10449. - ISSN 1422-0067(eISSN), DOI 10.3390/ijms221910449
Примечания : Cited References:45. - The research was partially funded by the Ministry of Science and Higher Education of the Russian Federation (projects No. FSRZ-2020-0006); by the RFBR and Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science (projects No. 20-44-243002 and 20-44-240006); and by the RFBR (project No. 20-34-90118).
Предметные рубрики: TRYPTOPHAN FLUORESCENCE
CRYSTAL-STRUCTURE
SUBUNIT
BIOLUMINESCENCE
Аннотация: Detecting the folding/unfolding pathways of biological macromolecules is one of the urgent problems of molecular biophysics. The unfolding of bacterial luciferase from Vibrio harveyi is well-studied, unlike that of Photobacterium leiognathi, despite the fact that both of them are actively used as a reporter system. The aim of this study was to compare the conformational transitions of these luciferases from two different protein subfamilies during equilibrium unfolding with urea. Intrinsic steady-state and time-resolved fluorescence spectra and circular dichroism spectra were used to determine the stages of the protein unfolding. Molecular dynamics methods were applied to find the differences in the surroundings of tryptophans in both luciferases. We found that the unfolding pathway is the same for the studied luciferases. However, the results obtained indicate more stable tertiary and secondary structures of P. leiognathi luciferase as compared to enzyme from V. harveyi during the last stage of denaturation, including the unfolding of individual subunits. The distinctions in fluorescence of the two proteins are associated with differences in the structure of the C-terminal domain of alpha-subunits, which causes different quenching of tryptophan emissions. The time-resolved fluorescence technique proved to be a more effective method for studying protein unfolding than steady-state methods./p
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nemtseva E. V., Gulnov D. V., Gerasimova M. A., Sukovatyi L. A., Burakova L. P., Karuzina N. E., Melnik B. S., Kratasyuk V. A.
Заглавие : Bacterial luciferases from vibrio harveyi and photobacterium leiognathi demonstrate different conformational stability as detected by time-resolved fluorescence spectroscopy
Место публикации : Int. J. Mol. Sci.: MDPI, 2021. - Vol. 22, Is. 19. - Ст.10449. - ISSN 16616596 (ISSN), DOI 10.3390/ijms221910449
Аннотация: Detecting the folding/unfolding pathways of biological macromolecules is one of the urgent problems of molecular biophysics. The unfolding of bacterial luciferase from Vibrio harveyi is well-studied, unlike that of Photobacterium leiognathi, despite the fact that both of them are actively used as a reporter system. The aim of this study was to compare the conformational transitions of these luciferases from two different protein subfamilies during equilibrium unfolding with urea. Intrinsic steady-state and time-resolved fluorescence spectra and circular dichroism spectra were used to determine the stages of the protein unfolding. Molecular dynamics methods were applied to find the differences in the surroundings of tryptophans in both luciferases. We found that the unfolding pathway is the same for the studied luciferases. However, the results obtained indicate more stable tertiary and secondary structures of P. leiognathi luciferase as compared to enzyme from V. harveyi during the last stage of denaturation, including the unfolding of individual subunits. The distinctions in fluorescence of the two proteins are associated with differences in the structure of the C-terminal domain of ?-subunits, which causes different quenching of tryptophan emissions. The time-resolved fluorescence technique proved to be a more effective method for studying protein unfolding than steady-state methods. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova, Tatiana, Baranovsky, Sergey, Petrovskaya, Olga, Shumilova, Anna, Sukovatyi, Alexey
Заглавие : Biological effects of the free and embedded metribuzin and tribenuron-methyl herbicides on various cultivated weed species
Колич.характеристики :11 с
Коллективы : Project "Agropreparations of the new generation: a strategy of construction and realization" [074-02-2018-328]; Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning" [220]
Место публикации : J. Environ. Sci. Health Part B-Pestic. Contam. Agric. Wastes: TAYLOR & FRANCIS INC, 2020. - ISSN 0360-1234, DOI 10.1080/03601234.2020.1807835. - ISSN 1532-4109(eISSN)
Примечания : Cited References:42. - This work was supported by Project "Agropreparations of the new generation: a strategy of construction and realization" [Agreement No 074-02-2018-328] in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning".
Предметные рубрики: SYNTHASE-INHIBITING HERBICIDES
CHLOROPHYLL FLUORESCENCE
RELEASE
Аннотация: The present study addresses the herbicidal activity and biological effects of the metribuzin (MET) and tribenuron-methyl (TBM) herbicides used to control various weed species (Amaranthus retroflexus, Sinapis arvensis,andLeucanthemum maximum). The effects of the free herbicides and the herbicides embedded in granules of degradable polymer poly-3-hydroxybutyrate [P(3HB)] blended with birch wood flour were compared. Metribuzin, regardless of the form, caused 100% mortality of the three weeds by day 21. The herbicidal activity of tribenuron-methyl was lower than that of metribuzin, but the embedded TBM was superior to the free herbicide in the length and strength of its action on the weeds. Both metribuzin forms dramatically decreased the main parameters of fluorescence: maximum quantum yield of photosystem-II [Y(II)(max)], maximum quantum yield of non-photochemical quenching [Y(NPQ)(max)], and maximum rate of non-cyclic electron transport [ETRmax] and concentrations of chlorophyllaandb. The effect of the embedded TBM on the photosynthetic activity of the weeds was lower in the first two weeks of the growth of herbicide-treated plants but lasted longer than the effect of the free TBM and increased over time. Embedding of metribuzin in the matrix of degradable blend did not decrease its herbicidal activity.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T., Baranovsky S., Petrovskaya O., Shumilova A., Sukovatyi A.
Заглавие : Biological effects of the free and embedded metribuzin and tribenuron-methyl herbicides on various cultivated weed species
Место публикации : J. Environ. Sci. Health Part B Pestic. Food Contamin. Agric. Wastes: Taylor and Francis Inc., 2020. - Article in press. - ISSN 03601234 (ISSN), DOI 10.1080/03601234.2020.1807835
Аннотация: The present study addresses the herbicidal activity and biological effects of the metribuzin (MET) and tribenuron-methyl (TBM) herbicides used to control various weed species (Amaranthus retroflexus, Sinapis arvensis, and Leucanthemum maximum). The effects of the free herbicides and the herbicides embedded in granules of degradable polymer poly-3-hydroxybutyrate [P(3HB)] blended with birch wood flour were compared. Metribuzin, regardless of the form, caused 100% mortality of the three weeds by day 21. The herbicidal activity of tribenuron-methyl was lower than that of metribuzin, but the embedded TBM was superior to the free herbicide in the length and strength of its action on the weeds. Both metribuzin forms dramatically decreased the main parameters of fluorescence: maximum quantum yield of photosystem-II [Y(II)max], maximum quantum yield of non-photochemical quenching [Y(NPQ)max], and maximum rate of non-cyclic electron transport [ETRmax] and concentrations of chlorophyll a and b. The effect of the embedded TBM on the photosynthetic activity of the weeds was lower in the first two weeks of the growth of herbicide-treated plants but lasted longer than the effect of the free TBM and increased over time. Embedding of metribuzin in the matrix of degradable blend did not decrease its herbicidal activity. © 2020 Taylor & Francis Group, LLC.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Eremeeva, Elena V., Vysotski, Eugene S.
Заглавие : Bioluminescent and biochemical properties of Cys-free Ca2+-regulated photoproteins obelin and aequorin
Колич.характеристики :9 с
Коллективы : Russian Academy of Sciences [03562016-0712, 0356-2015-0103]; RFBR [17-04-00764]
Место публикации : J. Photochem. Photobiol. B-Biol.: ELSEVIER SCIENCE SA, 2017. - Vol. 174. - С. 97-105. - ISSN 1011-1344, DOI 10.1016/j.jphotobio1.2017.07.021
Примечания : Cited References:54. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences (projects 03562016-0712 and 0356-2015-0103) and the RFBR grant 17-04-00764.
Предметные рубрики: SEQUENCE-ANALYSIS
APO-OBELIN
INTRINSIC FLUORESCENCE
COELENTERAZINE
Ключевые слова (''Своб.индексиров.''): bioluminescence--coelenterazine--photoprotein--coelenteramide--cysteine--serine
Аннотация: Bioluminescence of a variety of marine coelenterates is determined by Ca2+-regulated photoproteins. A strong interest in these proteins is for their wide analytical potential as intracellular calcium indicators and labels for in vitro binding assays. The presently known hydromedusan Ca2+-regulated photoproteins contain three (aequorin and clytin) or five (obelin and mitrocomin) cysteine residues with one of them strictly conserved. We have constructed Cys-free aequorin and obelin by substitution of all cysteines to serine residues. Such mutants should be of interest for researchers by the possibility to avoid the incubation with dithiothreitol (or p-mercaptoethanol) required for producing an active photoprotein that is important for some prospective analytical assays in which the photoprotein is genetically fused with a target protein sensitive to the reducing agents. Cys-free mutants were expressed in Escherichia coil, purified, and characterized regarding the efficiency of photoprotein complex formation, functional activity, and conformational stability. The replacement of cysteine residues has been demonstrated to affect different properties of aequorin and obelin. Cys-free aequorin displays a two-fold lower specific bioluminescence activity but preserves similar activation properties and light emission kinetics compared to the wild -type aequorin. In contrast, Cys-free obelin retains only 10% of the bioluminescence activity of wild-type obelin as well as binding coelenterazine and forming active photoprotein much less effectively. In addition, the substitution of Cys residues drastically changes the bioluminescence kinetics of obelin completely eliminating a "fast" component from the light signal decay curve. At the same time, the replacement of Cys residues increases conformational flexibility of both aequorin and obelin molecules, but again, the effect is more prominent in the case of obelin. The values of thermal midpoints of unfolding (Tm) were determined to be 53.3 0.2 and 44.6 0.4 C for aequorin and Cys-free aequorin, and 49.1 0.1 and 28.8 0.3 C for obelin and Cys-free obelin, respectively. Thus, so far only Cys-free aequorin is suitable as a partner for fusing with a tag sensitive to reducing agents since the aequorin mutant preserves almost 50% of the bioluminescent activity and can be produced with a substantial yield.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Belogurova N..., Kudryasheva N...
Заглавие : Change of fluorescence spectra of discharged photoprotein obelin under variation of physico-chemical conditions
Колич.характеристики :1 с
Место публикации : Luminescence: WILEY-BLACKWELL, 2014. - Vol. 29. - С. 63-63. - ISSN 1522-7235. - ISSN 1522-7243
Примечания : Cited References: 6
Предметные рубрики: PECULIARITIES
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14.

15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vetrova E.V., Kudryasheva N.S., Visser AJWG, van Hoek A...
Заглавие : Characteristics of enclogenous flavin fluorescence of Photobacterium leiognathi luciferase and Vibrio fischeri NAD(P)H : FMN-oxidoreductase
Колич.характеристики :5 с
Место публикации : Luminescence: JOHN WILEY & SONS LTD, 2005. - Vol. 20: 11th International Symposium on Luminescence Spectrometry - Detection Techniques in Biomedical and Environmental Analysis (MAY 05-08, 2004, Beijing, JAPAN), Is. 3. - P205-209. - ISSN 1522-7235, DOI 10.1002/bio.815
Примечания : Cited References: 22
Предметные рубрики: FLAVODOXIN
ANISOTROPY
REDUCTASE
DYNAMICS
SYSTEM
Ключевые слова (''Своб.индексиров.''): bacterial bioluminescence--flavin fluorescence
Аннотация: The bioluminescent bacterial enzyme system NAD(P)H:FMN-oxidoreductase-luciferase has been used as a test system for ecological monitoring. One of the modes to quench bioluminescence is the interaction of xenobiotics with the enzymes, which inhibit their activity. The use of endogenous flavin fluorescence for investigation of the interactions of non-fluorescent compounds with the bacterial luciferase from Photobacterium leiognathi and NAD(P)H:FMN-oxidoreductase from Vibrio fischeri has been proposed. Fluorescence spectroscopy methods have been used to study characteristics of endogenous flavin fluorescence (fluorophore lifetime, the rotational correlation time). The fluorescence anisotropy behaviour of FMN has been analysed and compared to that of the enzyme-bound flavin. The fluorescence characteristics of endogenous flavin of luciferase and NAD(P)H:FMN-oxidoreductase have been shown to be applicable in studying enzymes' interactions with non-fluorescent compounds. Copyright (c) 2005 John Wiley & Sons, Ltd.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zavorueva E.N., Ushakova S.A.
Заглавие : Characteristics of slow induction curve of chlorophyll fluorescence and CO2 exchange for the assessment of plant heat tolerance at various levels of light intensity
Колич.характеристики :8 с
Место публикации : Russ. J. Plant Physiol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2004. - Vol. 51, Is. 3. - P294-301. - ISSN 1021-4437, DOI 10.1023/B:RUPP.0000028674.39572.1c
Примечания : Cited References: 18
Ключевые слова (''Своб.индексиров.''): triticum aestivum--raphanus sativus var. minor--fluorescence--heat tolerance--pigments--co2 exchange
Аннотация: The heat tolerance of wheat (Triticum aestivum L.) and radish (Raphanus sativus L. var. minor) cenoses exposed to elevated and damaging air temperatures (35 degreesC for 20 h, 45 degreesC for 7 h) under photoculture conditions at various levels of photosynthetically active radiation (PAR) was assessed by measuring characteristics of the slow induction curve of chlorophyll fluorescence at 682 and 734 nm and the CO2 exchange rate. Irrespective of the illumination level, the exposure of the cenoses to 35 degreesC did not induce irreversible changes in the plant photosynthetic apparatus. The lowest extent of damage to wheat and radish cenoses exposed to 45 degreesC was observed at 150 W/m(2) of PAR, whereas the highest damage of the plants was observed at an illumination level that was close to the compensation point of the cenose photosynthesis (50-70 W/m(2) of PAR at air temperature of 24 degreesC). Viability index proved to be the most sensitive. characteristic, compared to other characteristics, which were determined by measuring the slow phase of fluorescence induction at 682 and 734 nm. In the cenoses studied, the pattern of changes in the viability index in response to a stress factor was close to the changes in the photosynthetic rate.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nesterenko, T. V., Shikhov, V. N., Tikhomirov, A. A.
Заглавие : Chlorophyll fluorescence as an indicator of age-dependent changes in photosynthetic apparatus of wheat leaves
Колич.характеристики :7 с
Коллективы : section VI of the Program for Basic Research of the State Academies of Sciences [56.1.4]
Место публикации : Russ. J. Plant Physiol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2015. - Vol. 62, Is. 3. - С. 307-313. - ISSN 1021-4437, DOI 10.1134/S1021443715020144. - ISSN 1608-3407(eISSN)
Примечания : Cited References:30. - This work was supported by the State Assignment, topic no. 56.1.4, section VI of the Program for Basic Research of the State Academies of Sciences for 2013-2020.
Предметные рубрики: LEAF SENESCENCE
ONTOGENIC APPROACH
LIGHT DEPENDENCE
PARAMETERS
Ключевые слова (''Своб.индексиров.''): triticum sativus--chlorophyll fluorescence parameters--actinic light--intensity--leaf ontogeny
Аннотация: Wheat (Triticum sativus L.) seedlings of various ages (2- to 16-day-old plants) were used to study age-dependent changes in the chlorophyll fluorescence induction (CFI) at various light intensities during flu- orescence measurements. Plants were raised in a growth chamber using hydroponics with expanded clay, controlled environmental conditions, and 690 A mu mol/(m(2) s) photon flux density (PFD) of photosynthetically active radiation (PAR). Parameters of CFI were determined under actinic PFD of 380, 580, 820, and 1340 A mu mol/(m(2) s) PAR. The fifth leaf from the stem base, exposed to uniform lighting, was sampled for measurements. This leaf emerged at the plant age of 16 days. Based on fluorescence data, we calculated the maximal photochemical quantum yield of photosystem II (F (v)/F (m)), the effective photochemical quantum yield of PSII (Yield), parameters of photochemical (qP) and non-photochemical (qN and NPQ) quenching of chlorophyll fluorescence, the F (p)/F (t) ratio, and the "vitality index" (fluorescence decrease ratio, R (fd)). At moderate actinic PFD, applied commonly in PAM fluorometers (about 380 A mu mol/(m(2) s)), age-dependent changes in NPQ, F (p)/F (t), and R (fd) were observed. Analysis of CFI parameters in wheat leaves of different ages at PFD increasing from 380 to 820 A mu mol/(m(2) s) revealed that R (fd), NPQ, and qN are the most sensitive markers of the leaf age among all parameters tested. These suitable indicators can be used for rapid assessment of the leaf age.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nesterenko T.V., Tikhomirov A.A., Shikhov V.N.
Заглавие : Chlorophyll fluorescence induction and estimation of plant resistance to stress factors
Место публикации : Zhurnal Obshchei Biologii. - 2007. - Vol. 68, Is. 6. - С. 444-458. - ISSN 00444596 (ISSN)
Ключевые слова (''Своб.индексиров.''): chlorophyll--adaptation--cucumber--fluorescence--growth, development and aging--metabolism--physiology--plant leaf--review--adaptation, physiological--chlorophyll--cucumis sativus--fluorescence--plant leaves--cucumis sativus
Аннотация: The usage of chlorophyll fluorescence induction (CFI) for estimating various types of plant resistance (primary, general, initial, adaptive) to stress factors is reviewed. The necessity of ontogenetic approach (considering the age-specific properties of the photosynthetic apparatus) in determining general and adaptive resistance of plants to prolonged action of stress factors by the CFI method is argued. In the plant Cucumbis sativus L., the possibility is shown of using age-specific qualitative and quantitative traits of leaf CFI (changes in the shape of chlorophyll fluorescence induction curves and in the dynamics of CFI parameters in the course of leaf ontogeny) for comparative study of differences between fully active and stressed plants. Possible criteria are suggested for estimating the effect of outer stress factors by the presence or absence of a steady-state phase in the dynamics of CFI parameters during leaf ontogeny. It is also suggested to use the duration of the steady-state phase following the termination of leaf growth (estimated by the dynamics of the slow phase of CFI as the ratio of fluorescence intensity at the peak P and the steady-state fluorescence intensity, FP/FS, or as the viability index Rfd) and the variability of CFI parameters during this period as qualitative estimates of plant resistance to prolonged action of stress factors.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : NESTERENKO T.V., TIKHOMIROV A.A., ZOLOTUKHIN I.G., VOLKOVA E.K.
Заглавие : CHLOROPHYLL INDUCTION FLUORESCENCE AND CERTAIN CHARACTERISTICS OF CUCUMBER LEAVES GROWN UNDER ACTION OF LIGHT OF VARIOUS SPECTRAL CONTENT AND INTENSITIES
Колич.характеристики :6 с
Место публикации : FIZIOLOGIYA I BIOKHIMIYA KULTURNYKH RASTENII: INST PLANT PHYSIOL GENETICS, 1985. - Vol. 17, Is. 1. - P11-16. - ISSN 0256-1425
Примечания : Cited References: 15
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolmakov V.I., Levin L.A., Dubovskaia O.P., Gladyshev M.I.
Заглавие : Circadian rhythms of feeding with microalgae of Ceriodaphnia quadrangula in laboratory culture
Место публикации : Biofizika. - 2002. - Vol. 47, Is. 4. - С. 673-676. - ISSN 00063029 (ISSN)
Ключевые слова (''Своб.индексиров.''): chlorophyll--alga--animal--article--chemistry--circadian rhythm--crustacea--fluorometry--growth, development and aging--photoperiodicity--physiology--algae--animals--chlorophyll--circadian rhythm--crustacea--fluorometry--photoperiod
Аннотация: The circadian rhythm of grazing of microalgae by a laboratory culture of Ceriodaphnia quadrangula under continuous illumination was studied by continuous registration of chlorophyll fluorescence at the outlet of a flow-through cultivator. A culture of green alga Chlorella vulgaris was used as a feed. The data obtained were treated by the statistical spectral analysis. It was found that animals preliminarily grown under a 12 h light: 12 h dark regime and transferred to constant light showed two maxima in the circadian rhythm of grazing with periods of 0.7 and 1.1 h. Animals preliminarily grown under constant light showed no circadian rhythm of grazing. It was concluded that the circadian rhythm of grazing of C. quadrangula has endogenous nature and can change according to light conditions.
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