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1.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondareva L.G., Bolsunovskii A.Ya., Sukhorukov F.V., Kazbanov V.I., Makarova I.V., Legler E.V.
Заглавие : Model assessment of the migration capability of transuranium radionuclides (241Am and Pu isotopes) and 152Eu in the system bottom sediments-Yenisei river water by chemical fractionation technique
Место публикации : Radiochemistry. - 2005. - Vol. 47, Is. 4. - С. 415-421. - ISSN 10663622 (ISSN) , DOI 10.1007/s11137-005-0112-8
Аннотация: Sorption of radiotracers 241Am and 242Pu and weighable amounts of uranium and stable Eu on bottom sediments in the simulated system bottom sediments-Yenisei river water was studied. At a contact time of 40 min these metals are completely sorbed on the bottom sediments, presumably via association with organomineral complexes and hydrated gels. Along with sorption, these metals can form soluble complexes and stable colloids (pseudocolloids). The distribution factors of 241Am and 242Pu, and stable Eu in the simulated system between bottom sediments and liquid phases were calculated from the results of sorption experiments. The introduced 241Am and 152Eu initially present in radioactively contaminated bottom sediments of the Yenisei river show similar pattern of distribution over differently mobile fractions. The distribution of weighable amounts of stable Eu significantly differs from that of initially present 152Eu and tracer 241Am due to decelerated sorption of stable Eu and its considerably higher concentration. A significant part of 242Pu, 241Am, and 152Eu is associated with mobile fractions of bottom sediments, which, under certain environmental conditions, can pass into river water as migrating species. В©2005 Pleiades Publishing, Inc.
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2.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bolsunovsky A.
Заглавие : Chemical fractionation of radionuclides and stable elements in aquatic plants of the yenisei river
Место публикации : Environmental Science and Technology. - 2011. - Vol. 45, Is. 17. - С. 7143-7150. - ISSN 0013936X (ISSN) , DOI 10.1021/es2008853
Ключевые слова (''Своб.индексиров.''): aquatic plants--artificial radionuclides--cell structure--chemical fractionation--distribution patterns--nuclear plant--plant biomass--sequential extraction scheme--stable elements--submerged plants--transuranic elements--americium--biomass--neptunium--river pollution--rivers--radioisotopes--americium--element--neptunium--radioisotope--bioaccumulation--chemical binding--gamma ray spectrometry--isotopic fractionation--monocotyledon--phytomass--radionuclide--river pollution--submerged vegetation--aquatic species--article--batrachium kauffmanii--biomass--cell structure--controlled study--extraction--fontinalis antipyretica--fractionation--gamma spectrometry--higher plant--nonhuman--plant growth--potamogeton lucens--potamogetonaceae--river--russian federation--aquatic organisms--biomass--chemical fractionation--china--industrial waste--plants--radiation monitoring--radioisotopes--rivers--water pollutants, radioactive--russian federation--yenisei river--batrachium--fontinalis antipyretica--potamogeton lucens
Аннотация: The Yenisei River is contaminated with artificial radionuclides released by one of the Russian nuclear plants. The aquatic plants growing in the radioactively contaminated parts of the river contain artificial radionuclides. The aim of the study was to investigate accumulation of artificial radionuclides and stable elements by submerged plants of the Yenisei River and estimate the strength of their binding to plant biomass by using a new sequential extraction scheme. The aquatic plants sampled were: Potamogeton lucens, Fontinalis antipyretica, and Batrachium kauffmanii. Gamma-spectrometric analysis of the samples of aquatic plants has revealed more than 20 radionuclides. We also investigated the chemical fractionation of radionuclides and stable elements in the biomass and rated radionuclides and stable elements based on their distribution in biomass. The greatest number of radionuclides strongly bound to biomass cell structures was found for Potamogeton lucens and the smallest for Batrachium kauffmanii. For Fontinalis antipyretica, the number of distribution patterns that were similar for both radioactive isotopes and their stable counterparts was greater than for the other studied species. The transuranic elements 239Np and 241Am were found in the intracellular fraction of the biomass, and this suggested their active accumulation by the plants. В© 2011 American Chemical Society.
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3.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondareva L., Bolsunovsky A.
Заглавие : Bioavailability of 99Tc to a macrophyte of the Yenisei River
Место публикации : Radioprotection. - 2009. - Vol. 44, Is. 5. - С. 71-76. - ISSN 00338451 (ISSN) , DOI 10.1051/radiopro/20095019
Аннотация: The experiments on accumulation of 99Tc by Elodea biomass showed that 99Tc activity concentration can reach 120 В± 6Bq/g, with the concentration factor for 99Tc 2700 В± 500 L/kg. In experiments on 99Tc release, over 504 h about 82% of the total 99Tc activity was released into the water from plant biomass; most of 99Tc was released within the first 192 h. Results of chemical fractionation of the biomass show that 99Tc contained in biomass was mainly concentrated in the exchangeable and the adsorbed fractions (83%). Thus, the data obtained using chemical fractionation of biomass confirmed the experimental data on 99Tc release, which suggested that most of the biomass-bound 99Tc was adsorbed on the biomass surface. 99Tc tightly bound to Elodea biomass (fractions of organics and mineral residue) constituted just 17% of the total 99Tc activity. Decreased illumination of Elodea shoots during the experiment did not cause any reduction in 99Tc activity concentration or concentration factor. Results of chemical fractionation of the biomass grown under lower illumination conditions show that the percentage of 99Tc tightly bound to Elodea biomass (fractions of organics and mineral residue) decreased while the 99Tc of the adsorbed fractions decreased. Our results and data reported by other authors suggest that some part of 99Tc activity can be bioavailable to living organisms and that the portion of bioavailable 99Tc can be determined by a number of factors. В© 2009 EDP Sciences.
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4.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bolsunovsky A., Bondareva L., Sukhorukov F., Melgunov M.
Заглавие : Accumulation of 242Pu by a macrophyte of the Yenisei River (Elodea canadensis) in laboratory experiments
Место публикации : Chemosphere. - 2009. - Vol. 75, Is. 3. - С. 284-288. - ISSN 00456535 (ISSN) , DOI 10.1016/j.chemosphere.2008.12.036
Ключевые слова (''Своб.индексиров.''): 242pu--accumulation--laboratory experiments--sequential extraction technique--submerged plant elodea canadensis--yenisei river--sup242/suppu--accumulation--laboratory experiments--sequential extraction technique--submerged plant elodea canadensis--yenisei river--biological materials--biomass--chemical plants--experiments--positive ions--rivers--plutonium--concentration (composition)--experimental study--laboratory method--macrophyte--plutonium isotope--river water--submerged vegetation--eurasia--russian federation--yenisei river--plutonium--article--biomass--chemistry--hydrocharitaceae--physiology--plant--radiation monitoring--river--time--water pollutant--biomass--hydrocharitaceae--plant shoots--plutonium--radiation monitoring--rivers--time factors--water pollutants, radioactive
Аннотация: The study addresses 242Pu accumulation by Elodea canadensis, one of the abundant species of submerged plants in the Yenisei River. 242Pu in water samples of the "Elodea - Yenisei River water" model system and in the biomass fractions was determined ?-spectrometrically, following radiochemical recovery of 242Pu using 236Pu - a chemical yield tracer. The experiments on accumulation of 242Pu by Elodea biomass showed that the activity concentration of 242Pu can reach 21 В± 2 Bq/g dry wt, with the concentration factor for 242Pu 13100 В± 2100 L/kg dry wt. Results of chemical fractionation proved that during the first few hours of the experiment 242Pu contained in Elodea was mainly concentrated in the exchangeable and the adsorbed fractions of biomass (about 100%). As Elodea biomass accumulated 242Pu, the absolute amount of 242Pu in the exchangeable and the adsorbed fractions remained almost unchanged, although the portion of 242Pu tightly bound to biomass increased. At the end of the experiment, on day 7, 242Pu tightly bound to biomass (fractions of organics and mineral residue) constituted 43-63% (in different experiments) of the total 242Pu in the biomass. В© 2008 Elsevier Ltd. All rights reserved.
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5.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bolsunovsky A., Bondareva L.
Заглавие : Accumulation and release of 99Tc by a macrophyte of the Yenisei River (Elodea canadensis) in laboratory experiments
Место публикации : Journal of Radioanalytical and Nuclear Chemistry. - 2008. - Vol. 277, Is. 3. - С. 631-636. - ISSN 02365731 (ISSN) , DOI 10.1007/s10967-007-7148-5
Ключевые слова (''Своб.индексиров.''): technetium 99m--aquatic flora--article--biomass--controlled study--dry weight--liquid scintillation counting--macrophyte--nonhuman--radiation absorption--radiation detection--radiation dose fractionation--radiation measurement--radioactivity--river--water sampling
Аннотация: The study addresses 99Tc accumulation and release by Elodea canadensis, one of the abundant species of submerged plants in the Yenisei River. 99Tc in water samples of the "Elodea - Yenisei River water" model system and in the biomass fractions was measured using a liquid scintillation analyzer. Experiments on accumulation of 99Tc by Elodea showed that 99Tc activity concentration can reach 120В±6 Bq/g dry wt, with the concentration factor for 99Tc 2700В±500 l/kg dry wt. In experiments on 99Tc release, over 504 hours about 82% of the total 99Tc activity was released into the water from the plant; most of 99Tc was released within the first 192 hours. The data obtained using sequential chemical fractionation of biomass confirmed the experimental data on 99Tc release, which suggested that most of the biomass-bound 99Tc was adsorbed on the surface of Elodea. 99Tc tightly bound to biomass (fractions of organics and mineral residue) constituted just 17% of the total 99Tc activity. В© 2008 Akademiai Kiado, Budapest.
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6.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bolsunovsky A., Zotina T., Bondareva L.
Заглавие : Accumulation and release of 241Am by a macrophyte of the Yenisei River (Elodea canadensis)
Место публикации : Journal of Environmental Radioactivity. - 2005. - Vol. 81, Is. 1. - С. 33-46. - ISSN 0265931X (ISSN) , DOI 10.1016/j.jenvrad.2004.10.012
Ключевые слова (''Своб.индексиров.''): 241am--accumulation--laboratory experiments--release--submerged plant elodea canadensis--yenisei river--activation analysis--biomass--concentration (process)--effluents--plutonium--rivers--aquatic plants--elodea plant--macrophytes--radioactive contamination--transuranium elements--americium 241--river water--americium--americium nitrate--bioaccumulation--biological uptake--macrophyte--pollutant source--radioactive pollution--river water--submerged vegetation--article--biomass--concentration (parameters)--environmental factor--fractionation--laboratory--macrophyte--radioactivity--river--sampling--adsorption--chemistry--hydrocharitaceae--methodology--physiology--plant--radiation exposure--radiation monitoring--russian federation--sediment--soil pollutant--time--tissue distribution--water pollutant--eastern hemisphere--eurasia--russian federation--world--yenisei river--elodea canadensis--adsorption--americium--biomass--chemical fractionation--geologic sediments--hydrocharitaceae--plant shoots--radiation monitoring--rivers--russia--soil pollutants, radioactive--time factors--tissue distribution--water pollutants, radioactive
Аннотация: The source of radioactive contamination of the Yenisei River floodplain, including contamination with transuranic elements, is the Mining-and-Chemical Combine of the Russian Ministry of Atomic Energy, which has for many years been producing weapons-grade plutonium. Transuranic elements have been detected not only in the soil and sediment of the river but also in the biomass of aquatic plants. This work is an investigation of accumulation and release of 241Am by a submerged macrophyte of the Yenisei River (Elodea canadensis) in laboratory experiments. In 2000-2003, laboratory experiments were carried out with biomass of E. canadensis Mich. and filtered river water. The samples were collected from the Yenisei River upstream of the discharge of the Combine's radioactive effluent. The experiments showed that 241Am is accumulated by Elodea biomass: the activity concentration of 241Am can reach 3280 В± 240 Bq/g, with the concentration factor for 241Am 16 600 В± 2200 l/kg. Results of chemical fractionation have proved that in the course of 241Am accumulation by Elodea biomass, 241Am tightly bound to biomass increases from 11% to 27% of the total 241Am in the biomass. Release of 241Am from the decaying Elodea biomass has been evaluated experimentally. By the end of the experiment (lasting up to 127 days), the Elodea plants had lost up to 65% of their initial 241Am activity and the rate of 241Am release into the water environment reached 23 Bq/day. В© 2004 Elsevier Ltd. All rights reserved.
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7.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bolsunovskij A.Ya., Zotina T.A., Bondareva L.G., Degermendzhi A.G.
Заглавие : An estimate of 241Am accumulation by the macrophyte Elodea Canadensis
Место публикации : Doklady Akademii Nauk. - 2004. - Vol. 399, Is. 1. - С. 130-133. - ISSN 08695652 (ISSN)
Ключевые слова (''Своб.индексиров.''): biomass--fractionation--minerals--organic compounds--plants (botany)--radioactivity--radioisotopes--accumulation--macrophytes--americium
Аннотация: The lab experiments showed that specific activity of 241Am achieves 3280В±240 Bc/g with 241Am accumulation coefficient = 16600В±2200 l/kg. The chemical fractionation results confirmed that 241Am can be strongly fixed by elodea biomass. At the end of long-term experiment the part of 241Am strongly bonded by the biomass reached 27% of total 241Am content in biomass. The accumulation process proceeds with 241Am redistribution towards decreasing of the part of exchange and adsorbed fractions and increasing of the part of organic and mineral fractions. Thus, the 241Am part in organic fractions doubled during the experiment and in mineral fraction - grew more than 10 times as compared with initial 241Am distribution.
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8.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zotina T.A., Kalacheva G.S., Bolsunovsky A.Y.
Заглавие : Biochemical fractionation and cellular distribution of americium and plutonium in the biomass of freshwater macrophytes
Место публикации : Journal of Radioanalytical and Nuclear Chemistry. - 2011. - Vol. 290, Is. 2. - С. 447-451. - ISSN 02365731 (ISSN) , DOI 10.1007/s10967-011-1228-2
Ключевые слова (''Своб.индексиров.''): 238, 242pu--241am--carbohydrates--cellulose--fractionation--protein--submerged macrophyte--americium 241--plutonium--plutonium 238--plutonium 242--polysaccharide--unclassified drug--article--bioaccumulation--biomass--cellular distribution--ceratophyllum demersum--controlled study--cytosol--elodea canadensis--fontinalis antipyretica--fractionation--freshwater species--macrophyte--moss--myriophyllum spicatum--nonhuman--plant cell--radiation absorption--radioactivity--shoot
Аннотация: Accumulation of americium ( 241Am) and plutonium ( 238,242Pu) and their distribution in cell compartments and biochemical components of the biomass of freshwater aquatic plants Elodea canadensis, Ceratophyllum demersum and Myrioplyllum spicatum and aquatic moss Fontinalis antipyretica have been investigated in laboratory experiments. Americium and plutonium taken up from water by Elodea canadensis apical shoots were mainly absorbed by structural components of plant cells (90% for 241Am; 89% for 238Pu and 82-87% for 242Pu). About 10-18% of isotope activity was recorded in the cytosol fraction. The major concentration (76-92%) of americium was bound to cell wall cellulose-like polysaccharides of Elodea canadensis, Myriophyllum spicatum, Ceratophyllum demersum and Fontinalis antipyretica, 8-24% of americium activity was registered in the fraction of proteins and carbohydrates, and just a minor concentration (<1%) in the lipid fraction. The distribution of plutonium in the biomass fractions of Elodea was similar to that of americium. Hence, americium and plutonium had the highest affinity to cellulose-like polysaccharides of cell walls of freshwater submerged macrophytes. В© 2011 Akademiai Kiado, Budapest, Hungary.
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9.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Sushchik N.N., Kalachova G.S., Makhutova O.N.
Заглавие : Stable isotope composition of fatty acids in organisms of different trophic levels in the yenisei river
Место публикации : PLoS ONE. - 2012. - Vol. 7, Is. 3. - Ст.e34059. - ISSN 19326203 (ISSN) , DOI 10.1371/journal.pone.0034059
Ключевые слова (''Своб.индексиров.''): carbon 13--fatty acid--carbon--apatania crymophila--article--benthos--eulimnogammarus viridis--fatty acid analysis--fatty acid desaturation--fontinalis antipyretica--food chain--food web--freshwater fish--isotope analysis--lipid composition--microalga--moss--nonhuman--pelagic zone--river ecosystem--russian federation--stable isotope analysis--thymallus arcticus--trophic level--animal--chemistry--larva--metabolism--river--bryophyta--gammaridae--trichoptera--animals--carbon isotopes--fatty acids--food chain--larva--microalgae--rivers
Аннотация: We studied four-link food chain, periphytic microalgae and water moss (producers), trichopteran larvae (consumers I), gammarids (omnivorous - consumers II) and Siberian grayling (consumers III) at a littoral site of the Yenisei River on the basis of three years monthly sampling. Analysis of bulk carbon stable isotopes and compound specific isotope analysis of fatty acids (FA) were done. As found, there was a gradual depletion in 13C contents of fatty acids, including essential FA upward the food chain. In all the trophic levels a parabolic dependence of ? 13C values of fatty acids on their degree of unsaturation/chain length occurred, with 18:2n-6 and 18:3n-3 in its lowest point. The pattern in the ? 13C differences between individual fatty acids was quite similar to that reported in literature for marine pelagic food webs. Hypotheses on isotope fractionation were suggested to explain the findings. В© 2012 Gladyshev et al.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Sushchik N.N., Makhutova O.N., Kalachova G.S.
Заглавие : Trophic fractionation of isotope composition of polyunsaturated fatty acids in the trophic chain of a river ecosystem
Место публикации : Doklad. Biochem. Biophys. - 2014. - Vol. 454, Is. 1. - С. 4-5. - ISSN 16076729 (ISSN) , DOI 10.1134/S1607672914010025
Предметные рубрики: Biochemistry, general
Biophysics and Biological Physics
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Dementyev D.V., Zotina T.A., Manukovsky N.S., Kalacheva G.S., Bolsunovsky, A. Ya.
Заглавие : Biosorption of Am-241 from aqueous solutions and its biochemical fractionation in Pleurotus ostreatus mycelium
Колич.характеристики :3 с
Коллективы : Russian Foundation for Basic Research [12-04-00915]
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2015. - Vol. 460, Is. 1. - С. 34-36. - ISSN 1607-6729, DOI 10.1134/S160767291501010X. - ISSN 1608-3091(eISSN)
Примечания : Cited References:11. - This work was supported by the Russian Foundation for Basic Research (project no. 12-04-00915).
Предметные рубрики: SACCHAROMYCES-CEREVISIAE
REMOVAL
AMERICIUM
MECHANISM
BIOMASS
WOS,
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M. I., Makhutova O. N., Kravchuk E. S., Anishchenko O. V., Sushchik N. N.
Заглавие : Stable isotope fractionation of fatty acids of Daphnia fed laboratory cultures of microalgae
Место публикации : Limnologica. - 2016. - Vol. 56. - С. 23-29. - ISSN 00759511 (ISSN) , DOI 10.1016/j.limno.2015.12.001
Ключевые слова (''Своб.индексиров.''): compound-specific isotope analysis--fatty acids--food webs--stable isotope fractionation--comparative study--crustacean--fatty acid--food web--isotopic analysis--isotopic fractionation--microalga--stable isotope--chlorella vulgaris--cryptomonas sp.--daphnia--daphnia galeata
Аннотация: We tested a comparatively new method of tracing of natural food webs, compound-specific isotope analysis (CSIA) of fatty acids (FA), using laboratory culture of Daphnia galeata fed Chlorella vulgaris and Cryptomonas sp. In general, Daphnia had significantly lighter carbon stable isotope composition of most fatty acids, including essential, than those of their food, microalgae. Thus, our results did not support the pivotal premise of the FA-CSIA application for food web analysis, i.e., transmitting the isotope 'signal' of essential FAs to consumers from their food without any modification. Moreover, the values of isotope fractionation of particular FAs in the consumer relative to its food were not constant, but varied from 1.35‰ to 7.04‰. The different isotope fractionation (depletion) values of diverse FAs in consumer were probably caused by different processes of their synthesis, catabolism and assimilation. More work is evidently to be done for correct interpretation of results of FA-CSIA during field studies for tracing of natural food webs. © 2015 Elsevier GmbH.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Dementyev D. V., Zotina T. A., Manukovsky N. S., Kalacheva G. S.
Заглавие : Biosorption of 241Am from solution and its biochemical fractionation in the mycelium of macromycetes
Место публикации : Radiochemistry. - 2015. - Vol. 57, Is. 6. - С. 661-665. - ISSN 10663622 (ISSN) , DOI 10.1134/S1066362215060144
Ключевые слова (''Своб.индексиров.''): americium--fungus mycelium--sorption
Аннотация: Experiments with macromycetes Pleurotus ostreatus, Neonothopanus nambi, and Agaricus bisporus demonstrated for the first time that live mycelium of mushrooms, cultivated on a liquid culture medium, can efficiently take up dissolved 241Am in its biomass. Biochemical fractionation of the mycelium biomass demonstrated for the first time that the major fraction (up to 90%) of 241Am accumulated in the mycelium is bonded to structural polysaccharides of the cell walls. The sorption capacity of cell wall polysaccharides for 241Am is 2.7-3.6 times higher than that of the initial mycelium biomass, and the activity concentration of 241Am in the polysaccharides reached 950 Bq g-1 dry weight. © 2015 Pleiades Publishing, Inc.
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