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Вид документа : Статья из журнала
Шифр издания : 577.151.45/П 65
Автор(ы) : Пуртов К.В., Петушков В.Н., Родионова Н.С., Гительзон И.И.
Заглавие : Почему у биолюминесцентного опёнка осеннего (Armillaria mellea) светится мицелий, но не светится плодовое тело? : статья
Место публикации : Доклады Академии наук. - Москва: Федеральное государственное унитарное предприятие Академический научно-издательский, производственно-полиграфический и книгораспространительский центр Наука, 2017. - Т. 474, № 4. - С. 510-512. - ISSN 0869-5652, DOI 10.7868/S0869565217040247
УДК : 577.151.45
Аннотация: С помощью определения компонентов-участников биолюминесцентного процесса в светящихся и несветящихся органах опёнка осеннего выяснили причины неполного свечения Armillaria mellea. Полный набор ферментов и субстратов, необходимых для биолюминесценции, образуется только в мицелии, причём исключительно в условиях свободного доступа кислорода. В плодовых телах синтез предшественника люциферина (гиспидина) и 3-гидроксигиспидингидроксилазы блокирован, вследствие этого и не происходило образование люциферина - ключевого компонента биолюминесцентной системы грибов. Именно поэтому плодовое тело опёнка осеннего не светится, несмотря на наличие люциферазы - фермента, катализирующего окисление люциферина с испусканием кванта света.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Purtov K. V., Petushkov V. N., Rodionova N. S., Gitelson J. I.
Заглавие : Why does the bioluminescent fungus Armillaria mellea have luminous mycelium but nonluminous fruiting body?
Место публикации : Doklad. Biochem. Biophys.: Maik Nauka Publishing / Springer SBM, 2017. - Vol. 474, Is. 1. - С. 217-219. - ISSN 16076729 (ISSN) , DOI 10.1134/S1607672917030176
Аннотация: By determining the components involved in the bioluminescence process in luminous and nonluminous organs of the honey fungus Armillaria mellea, we have established causes of partial luminescence of this fungus. The complete set of enzymes and substrates required for bioluminescence is formed only in the mycelium and only under the conditions of free oxygen access. Since the synthesis of luciferin precursor (hispidin) and 3-hydroxyhispidin hydroxylase in the fruiting bodies is blocked, the formation of luciferin—the key component of fungal bioluminescent system—was not observed. That is why the fruiting body of Armillaria mellea is nonluminous despite the presence of luciferase, the enzyme that catalyzes the oxidation of luciferin with a photon emission. © 2017, Pleiades Publishing, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Puzyr A. P., Medvedeva S. E., Bondar V. S.
Заглавие : The use of glowing wood as a source of luminescent culture of fungus mycelium
Колич.характеристики :17 с
Коллективы : RF Government [11.G34.31.0058]; SB RAS [71]
Место публикации : Mycosphere: MYCOSPHERE PRESS, 2016. - Vol. 7, Is. 1. - С. 1-17. - ISSN 2077-7000, DOI 10.5943/mycosphere/7/1/1
Примечания : Cited References:22. - The authors are grateful to Prof. A. Frank, Director of North Borneo Biostation, for the opportunity to carry out studies of glowing wood; to Nadezhda N. Kudashova, a senior researcher at the Institute of Biology and Biophysics at the Tomsk University, for identifying the species of nonluminous fungi. This study was supported by grant no. 11.G34.31.0058 (RF Government) and Projects no. 71 (SB RAS).
Предметные рубрики: BIOLUMINESCENCE CHARACTERISTICS
NEONOTHOPANUS-NAMBI
LIGHT-EMISSION
Ключевые слова (''Своб.индексиров.''): bioluminescence--culture of luminous mycelia--kinetics of luminescent--reaction--light emitting wood--luminous fungus
Аннотация: In studies of fungal bioluminescence, not only fruiting bodies and spores of the fungus, but also samples of luminescent wood, leaf litter or soil may need to be used to derive pure mycelial culture. This study describes an approach to isolating the culture of luminescent fungal mycelium from samples of light-emitting wood found on Borneo Island in November-December 2013. A GelDoc XR Imaging System (Bio-Rad Laboratories, Inc., U.S.) was used for the first time to monitor luminescence and select luminous samples. This study shows that for successful isolation of the culture of luminescent mycelium out of the luminescent wood found in the forest, it is imperative to keep the samples moist (mycelium alive until there is water), while immediate and aseptic delivery of the samples to the laboratory is not a crucial condition (inner layers of wood is "sterile"). Investigation of the growth features of the isolated mycelium in various growing conditions revealed some peculiar properties of its luminescence in comparison with the known luminescent cultures of basidiomycetes. When grown on solid nutrient media, mycelium exhibits low growth rates, long-lasting luminescence (140 days or longer), and emergence and disappearance of local zones with high levels of light emission. Mycelium produced in submerged culture does not emit light, and this effect must be caused by the absence or a very low level of the luminescent reaction substrate in the biomass. The luminescence system isolated from mycelial biomass did not induce luminescent reaction in vitro upon the addition of NADPH (recording intensity is 60 100 URL/sec). We found that enzymes of the luminescence systems isolated from mycelium pellets retained their activity and catalyzed luminescent reaction when a hot extract of the luminous fungus Armillaria sp. (IBSO 2360) was added (near 1900 URL/sec). The same effect was obtained after addition of hot extracts from the fruiting bodies of nonluminous higher fungi Pholiota squarrosa, Cortinarius sp., Hypholoma capnoides and Chroogomphus rutilus (near 3500 URL/sec). The pure culture of luminescent mycelium has been registered in the Culture Collection of IBP SB RAS as IBSO 2371; now it can be used for various in vivo and in vitro studies, including identification of the fungus.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gorokhovatsky A. Y., Chepurnykh T. V., Shcheglov A. S., Mokrushina Y. A., Baranova M. N., Goncharuk S. A., Purtov K. V., Petushkov V. N., Rodionova N. S., Yampolsky I. V.
Заглавие : The Recombinant Luciferase of the Fungus Neonothopanus nambi: Obtaining and Properties
Место публикации : Doklad. Biochem. Biophys.: Pleiades journals, 2021. - Vol. 496, Is. 1. - С. 52-55. - ISSN 16076729 (ISSN), DOI 10.1134/S1607672921010051
Аннотация: Abstract: A key component of the recently described bioluminescent system of higher fungi is luciferase, a new class of proteins. The properties of fungal luciferase and their relationship with its structure are interesting both for improving autoluminescent systems already created on its basis and for creating new ones. Therefore, it is extremely important to understand the spatial structure of this protein. We have performed heterologous expression and purification of Neonothopanus nambi luciferase, obtained a protein suitable for subsequent crystallization, and also determined some biochemical properties of the recombinant luciferase. © 2021, The Author(s),.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gorokhovatsky, A. Yu, Chepurnykh, T., V, Shcheglov A. S., Mokrushina, Yu A., Baranova M. N., Goncharuk S. A., Purtov, K., V, Petushkov V. N., Rodionova N. S., Yampolsky, I., V
Заглавие : The Recombinant Luciferase of the Fungus Neonothopanus nambi: Obtaining and Properties
Колич.характеристики :4 с
Коллективы : Russian Science FoundationRussian Science Foundation (RSF) [16-14-00052-P]; President's grant for state support of the leading scientific schools of the Russian Federation [NSh-2605.2020.4]
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2021. - Vol. 496, Is. 1. - С. 52-55. - ISSN 1607-6729, DOI 10.1134/S1607672921010051. - ISSN 1608-3091(eISSN)
Примечания : Cited References:10. - The work was supported by the Russian Science Foundation (project no. 16-14-00052-P). The creation of the luciferase-producing yeast strain nnLuz was supported by the President's grant for state support of the leading scientific schools of the Russian Federation (NSh-2605.2020.4).
Аннотация: A key component of the recently described bioluminescent system of higher fungi is luciferase, a new class of proteins. The properties of fungal luciferase and their relationship with its structure are interesting both for improving autoluminescent systems already created on its basis and for creating new ones. Therefore, it is extremely important to understand the spatial structure of this protein. We have performed heterologous expression and purification of Neonothopanus nambi luciferase, obtained a protein suitable for subsequent crystallization, and also determined some biochemical properties of the recombinant luciferase.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondar V.S., Puzyr A.P., Purtov K.V., Medvedeva S.E., Rodicheva E.K., Gitelson J.I.
Заглавие : The luminescent system of the luminous fungus Neonothopanus nambi
Место публикации : Doklady Biochemistry and Biophysics. - 2011. - Vol. 438, Is. 1. - С. 138-140. - ISSN 16076729 (ISSN) , DOI 10.1134/S1607672911030082
Ключевые слова (''Своб.индексиров.''): agaricales--article--chemistry--luminescence--agaricales--luminescence--fungi--neonothopanus nambi
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Furyaeva A.V., Shevchenko V.T., Pechurkin N.S.
Заглавие : The effect of temperature on the growth kinetics of Candida tropicalis 29-10 continuously cultivated in the pH-stat at different growth-limiting conditions
Место публикации : Prikladnaya Biokhimiya i Mikrobiologiya. - 1986. - Vol. 22, Is. 2. - С. 243-247. - ISSN 05551099 (ISSN)
Ключевые слова (''Своб.индексиров.''): candida tropicalis--continuous culture--fungus--growth--nonhuman--ph--temperature
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kobzeva T.V., Melnikov A.R., Karogodina T.Y., Zikirin S.B., Stass D.V., Molin Y.N., Rodicheva E.K., Medvedeva S.E., Puzyr A.P., Burov A.A., Bondar V.S., Gitelson J.I.
Заглавие : Stimulation of luminescence of mycelium of luminous fungus Neonothopanus nambi by ionizing radiation
Колич.характеристики :8 с
Коллективы : Program of Siberian Branch of Russian Academy of Sciences [71]; Council for Grants of the President of the Russian Federation for Support of Leading Scientific Schools [NSh 2272.2012.3]; Russian Foundation for Basic Research [12-03-33082]; Program of Government of Russian Federation "On the Efforts for Attracting Leading Researchers to Educational Institutions of Russia" [11.G34.31.0058]
Место публикации : Luminescence: WILEY-BLACKWELL, 2014. - Vol. 29, Is. 7. - С. 703-710. - ISSN 1522-7235, DOI 10.1002/bio.2656. - ISSN 1522-7243
Примечания : Cited References: 29. - The work was supported by the Program of Siberian Branch of Russian Academy of Sciences (project no. 71), Council for Grants of the President of the Russian Federation for Support of Leading Scientific Schools (project no. NSh 2272.2012.3), the Russian Foundation for Basic Research (project no. 12-03-33082), and the Program of Government of Russian Federation "On the Efforts for Attracting Leading Researchers to Educational Institutions of Russia" (grant no. 11.G34.31.0058).
Предметные рубрики: BIOLUMINESCENCE
COMPONENTS
MECHANISMS
SYSTEM
Ключевые слова (''Своб.индексиров.''): higher luminous fungi--neonothopanus nambi--ionizing irradiation--reactive oxygen species--lipid peroxidation
Аннотация: The luminescent system of higher luminous fungi is not fully understood and the enzyme/substrate pair of the light emission reaction has not been isolated. It was suggested that luminescence of fungi involves oxidase-type enzymes, and reactive oxygen species are important for fungal light production. Generation of reactive oxygen species can be stimulated by ionizing irradiation, which has not been studied for luminous fungi. We report the effect of X-irradiation on the luminescence of fungus Neonothopanus nambi. Experiments were performed withmyceliumon a home-built setup based on an X-ray tube and monochromator/photomultiplier tube. Application of X-rays does not change the emission spectrum, but after approximately 20 min of continuous irradiation, light production from unsupported mycelium starts growing and increases up to approximately five times. After peaking, its level decreases irrespective of the presence of X-irradiation. After staying at a certain level, light production collapses to zero, which is not related to the drying of the mycelium or thermal impact of radiation. The observed shape of kinetics is characteristic of a multistage and/or chain reaction. The time profile of light production must reflect the current levels of radicals present in the system and/or the activity of enzyme complexes involved in light production. Copyright (C) 2014 John Wiley & Sons, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ronzhin N. O., Mogilnaya O. A., Posokhina E. D., Bondar V. S.
Заглавие : Reusable System for Phenol Detection in an Aqueous Medium Based on Nanodiamonds and Extracellular Oxidase from Basidiomycete Neonothopanus nambi
Колич.характеристики :5 с
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2021. - Vol. 499, Is. 1. - С. 220-224. - ISSN 1607-6729, DOI 10.1134/S1607672921040141. - ISSN 1608-3091(eISSN)
Примечания : Cited References:15
Предметные рубрики: PEROXIDASES
EXPRESSION
Аннотация: A reusable system for phenol determination in an aqueous medium was obtained by adsorption of extracellular oxidase from fungus Neonothopanus nambi onto modified nanodiamonds (MND) synthesized by detonation. It was found that the enzyme strongly binds to MND and exhibits catalytic activity in the reaction of co-oxidation of phenol with 4-aminoantipyrine without the addition of hydrogen peroxide. In the presence of the MND-oxidase complex, a significantly (by an order of magnitude) higher yield of the reaction product is recorded as compared to the yield in the presence of a free enzyme; the mechanism of the revealed effect is discussed. Model experiments have demonstrated the multiple use of the MND-oxidase complex for testing phenol in aqueous samples. The immobilized enzyme exhibits functional activity during long-term (2 months) storage of the MND-oxidase complex at 4 degrees C. The data obtained create the prerequisites for using the created system in environmental monitoring of water pollution with phenol.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Beregovaya K. A., Myshkina N. M., Chepurnykh T. V., Kotlobay A. A., Purtov K. V., Petushkov V. N., Rodionova N. S., Yampolsky I. V.
Заглавие : Rational Design and Mutagenesis of Fungal Luciferase from Neonothopanus nambi
Место публикации : Doklad. Biochem. Biophys.: Pleiades journals, 2021. - Vol. 496, Is. 1. - С. 14-17. - ISSN 16076729 (ISSN), DOI 10.1134/S1607672921010026
Аннотация: Abstract: The recently described bioluminescent system from fungi has great potential for developing highly efficient tools for biomedical research. Luciferase enzyme is one of the most crucial components of this system. The luciferase from Neonothopanus nambi fungus belongs to the novel still undescribed protein family. The structure data for this protein is almost absent. A detailed study of the N. nambi luciferase properties is necessary for the improvement of analytical methods based on the fungal bioluminescent system. Here we present the positions of key amino acid residues and their effect on enzyme function described using bioinformatic and experimental approaches. These results are useful for further fungal luciferase structure determination. © 2021, Pleiades Publishing, Ltd.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Beregovaya K. A., Myshkina N. M., Chepurnykh, T., V, Kotlobay A. A., Purtov, K., V, Petushkov V. N., Rodionova N. S., Yampolsky, I., V
Заглавие : Rational Design and Mutagenesis of Fungal Luciferase from Neonothopanus nambi
Колич.характеристики :4 с
Коллективы : Russian Science FoundationRussian Science Foundation (RSF) [16-14-00052P]; President grant for leading scientific schoolsLeading Scientific Schools Program [NSh-2605.2020.4]
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2021. - Vol. 496, Is. 1. - С. 14-17. - ISSN 1607-6729, DOI 10.1134/S1607672921010026. - ISSN 1608-3091(eISSN)
Примечания : Cited References:12. - This work was supported by the grant from the Russian Science Foundation no. 16-14-00052P, alanine screening was performed by the President grant for leading scientific schools NSh-2605.2020.4.
Аннотация: The recently described bioluminescent system from fungi has great potential for developing highly efficient tools for biomedical research. Luciferase enzyme is one of the most crucial components of this system. The luciferase from Neonothopanus nambi fungus belongs to the novel still undescribed protein family. The structure data for this protein is almost absent. A detailed study of the N. nambi luciferase properties is necessary for the improvement of analytical methods based on the fungal bioluminescent system. Here we present the positions of key amino acid residues and their effect on enzyme function described using bioinformatic and experimental approaches. These results are useful for further fungal luciferase structure determination.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Khizhnyak S.V., Tausheva I.V., Berezikova A.A., Nesterenko E.V., Rogozin D.Yu.
Заглавие : Psychrophilic and psychrotolerant heterotrophic microorganisms of Middle Siberian karst cavities
Место публикации : Russian Journal of Ecology. - 2003. - Vol. 34, Is. 4. - С. 231-235. - ISSN 10674136 (ISSN) , DOI 10.1023/A:1024537513439
Ключевые слова (''Своб.индексиров.''): caves--heterotrophic microorganisms--karst cavities--psychorophilic and psychrotolerant microorganisms--bacterium--cavernicolous species--fungus--heterotrophy--karst--low temperature--russian federation--arthrobacter--bacteria (microorganisms)--chrysosporium--corynebacteriaceae--fungi--mucor--penicillium--pseudomonas
Аннотация: The natural microflora of Middle Siberian karst cavities, which comprises psychrotolerant bacteria and fungi capable of growing at 3-15 and 3...+28В°C, respectively, has been studied. Bacteria are ubiquitous in caves, their count varying from 103 to 107 cells/g ground. The bacteria have been identified as Pseudomonas, Arthrobacter, Bacillus, and coryneform bacteria. Fungi have been found in places exposed to increased anthropogenic impact, their count being as large as 106 to 107 cells/g ground. Mucor, Penicillium, and Chrysosporium were dominant fungal genera.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Polyakova G., Pashenova N., Senashova V., Podolyak N., Kudryasheva N.
Заглавие : Pine stands as bioindicators: Justification for air toxicity monitoring in an industrial metropolis
Место публикации : Environ. - MDPI: MDPI AG, 2020. - Vol. 7, Is. 4. - Ст.28. - ISSN 20763298 (ISSN), DOI 10.3390/environments7040028
Аннотация: Five permanent sample plots (SPs; 200-250 trees per plot) were established in middleaged high-grade suburban pine stands near the industrial city of Krasnoyarsk, Siberia, Russia. Needle damage, inventory parameters of the stands, and the defense response of the stem phloem were evaluated annually for the years 2002-2019 and attributed to acute or chronic toxic exposures (creeping fire or industrial pollutants, respectively). The results form a basis for using trees as bioindicators. A newly elaborated stem lesion test was formed from a hypothesis on the upward sugar transport for the regeneration of an injured crown, based on Eschrich‘s model of bidirectional sugar transport in the phloem. The formation of a phloem lesion was induced by inoculation of the stem with a mycelial extract of the ophiostomatoid fungus Ceratocystis laricicola. The lesion length and its shift relative to the inoculation hole were measured. An increase in the length of needles at early stages of stand weakening by pollutants was found to correspond to the hormesis model (Selye‘s adaptation syndrome). A possibility of assessing the chronology of pollutant toxicity and the duration of the recovery period after creeping fire was shown. © 2020 by the authors.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Polyakova, Galina, Pashenova, Natalia, Senashova, Vera, Podolyak, Natalia, Kudryasheva, Nadezhda
Заглавие : Pine Stands as Bioindicators: Justification for Air Toxicity Monitoring in an Industrial Metropolis
Колич.характеристики :13 с
Коллективы : Russian Academy of SciencesRussian Academy of Sciences [0356-2018-0742, 0356-2017-0017]; RFBRRussian Foundation for Basic Research (RFBR) [15-04-06575]; RFBR-Krasnoyarsk Regional Foundation [18-44-240004, 18-44-242002]
Место публикации : Environments: MDPI, 2020. - Vol. 7, Is. 4. - Ст.28. - ISSN 2076-3298(eISSN), DOI 10.3390/environments7040028
Примечания : Cited References:35. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences, projects 0356-2018-0742 and 0356-2017-0017, and by RFBR grant 15-04-06575, RFBR-Krasnoyarsk Regional Foundation N 18-44-240004, grant 18-44-242002.
Предметные рубрики: HORMESIS
Аннотация: Five permanent sample plots (SPs; 200-250 trees per plot) were established in middle-aged high-grade suburban pine stands near the industrial city of Krasnoyarsk, Siberia, Russia. Needle damage, inventory parameters of the stands, and the defense response of the stem phloem were evaluated annually for the years 2002-2019 and attributed to acute or chronic toxic exposures (creeping fire or industrial pollutants, respectively). The results form a basis for using trees as bioindicators. A newly elaborated stem lesion test was formed from a hypothesis on the upward sugar transport for the regeneration of an injured crown, based on Eschrich's model of bidirectional sugar transport in the phloem. The formation of a phloem lesion was induced by inoculation of the stem with a mycelial extract of the ophiostomatoid fungus Ceratocystis laricicola. The lesion length and its shift relative to the inoculation hole were measured. An increase in the length of needles at early stages of stand weakening by pollutants was found to correspond to the hormesis model (Selye's adaptation syndrome). A possibility of assessing the chronology of pollutant toxicity and the duration of the recovery period after creeping fire was shown.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondar V.S., Rodicheva E.K., Medvedeva S.E., Tyulkova N.A., Tyaglik A.B., Shpak B.A., Gitelson J.I.
Заглавие : On the mechanism of luminescence of the fungus Neonothopanus nambi
Место публикации : Doklady Biochemistry and Biophysics. - 2013. - Vol. 449, Is. 1. - С. 80-83. - ISSN 16076729 (ISSN) , DOI 10.1134/S1607672913020075
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mogilnaya, Olga, Ronzhin, Nikita, Artemenko, Karina, Bondar, Vladimir
Заглавие : Nanodiamonds as an effective adsorbent for immobilization of extracellular peroxidases from luminous fungus Neonothopanus nambi to construct a phenol detection system
Колич.характеристики :9 с
Коллективы : Russian Academy of Sciences [0356-2016-0709]
Место публикации : Biocatal. Biotransform.: TAYLOR & FRANCIS LTD, 2019. - Vol. 37, Is. 2. - С. 97-105. - ISSN 1024-2422, DOI 10.1080/10242422.2018.1472586. - ISSN 1029-2446(eISSN)
Примечания : Cited References:50. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences [project no. 0356-2016-0709].
Предметные рубрики: CARBON NANOTUBES
ARMILLARIA-BOREALIS
LIGHT-EMISSION
DEGRADATION
Ключевые слова (''Своб.индексиров.''): nanodiamonds--immobilization--luminous fungus--beta-glucosidase--peroxidase--indicator system
Аннотация: Modified nanodiamonds (MNDs) produced by detonation synthesis can be used as an effective adsorbent to immobilize extracellular peroxidases of the luminous basidiomycete Neonothopanus nambi. The enzymes are firmly immobilized on MND particles and exhibit catalytic activity. The indicator system (the MND-enzyme complex) reused many times retains its ability to catalyze reaction of co-oxidation of phenol and 4-aminoantipirine in the presence of hydrogen peroxide and remains functionally active during long-term storage (for 1 month or longer) in aqueous suspensions at 4 degrees C. MNDs and enzymes of higher fungi can be effectively used to construct new reusable indicator systems for analytical applications such as monitoring contamination of aquatic environments by phenolic compounds.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mogilnaya O. A., Ronzhin N. O., Artemenko K. S., Bondar V. S.
Заглавие : Morphological properties and levels of extracellular peroxidase activity and light emission of the basidiomycete Armillaria borealis treated with beta-glucosidase and chitinase
Колич.характеристики :12 с
Коллективы : Russian Academy of Sciences [0356-2016-0709, 0356-2015-0103]
Место публикации : Mycosphere: MYCOSPHERE PRESS, 2017. - Vol. 8, Is. 4. - С. 649-+. - ISSN 2077-7000, DOI 10.5943/mycosphere/8/4/11
Примечания : Cited References:39. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences (project no. 0356-2016-0709) and Program No. II. 2 "Integration and Development" of the Siberian Branch of the Russian Academy of Sciences (project no. 0356-2015-0103).
Предметные рубрики: FUNGAL CELL-WALL
OXIDATIVE STRESS
PHANEROCHAETE-CHRYSOSPORIUM
Ключевые слова (''Своб.индексиров.''): basidiomycetes--bioluminescence--cell wall--beta-glucosidase--chitinase--peroxidase
Аннотация: The study estimates morphological properties and levels of extracellular peroxidase activity and light emission of mycelium of the basidiomycete Armillaria borealis IBSO 2328 treated with beta-glucosidase and chitinase. Mycelium incubated with the enzymes shows considerable morphological changes and indications of osmotic shock. Injuries observed in the cell envelope of the fungal hyphae are primarily attributed to the partial (in the beta-glucosidase treatment) or complete (in the chitinase treatment) disintegration of the melanin layer on the surface of the cell wall. Changes in the cell wall of hyphae are accompanied by release of extracellular peroxidases of the fungus into the incubation medium and an increase in light emission relative to the luminescence of the control pellets. We assume that higher level of luminescence of the enzyme-treated mycelium samples could be related to the disintegration of the surface pigment layer of the hyphae and the partial loss of extracellular peroxidases. The data obtained confirm the previously proposed hypothesis in which light producing reaction of the fungus may be an additional way to neutralize active oxygen radicals under stress.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prudnikova S. V., Evgrafova S. Y., Volova T. G.
Заглавие : Metabolic activity of cryogenic soils in the subarctic zone of Siberia towards “green” bioplastics
Место публикации : Chemosphere: Elsevier Ltd, 2021. - Vol. 263. - Ст.128180. - ISSN 00456535 (ISSN), DOI 10.1016/j.chemosphere.2020.128180
Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic – poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9–11 °C, the total biomass of microorganisms at a 10-20-cm depth was 226–234 mg g?1 soil and CO2 production was 20–46 mg g?1 day?1. The total abundance of microscopic fungi varied between (7.4 ± 2.3) ? 103 and (18.3 ± 2.2) ? 103 CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 ± 0.1) ? 106 CFU g?1 soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique – plating of isolates on the mineral agar with polymer as sole carbon source – was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)-degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. © 2020 Elsevier Ltd
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Puzyr A. P., Medvedeva S. E.
Заглавие : Luminescence of wood samples during long-term storage
Место публикации : Mycosphere: Guizhou Key Laboratory of Agricultural Biotechnology, 2016. - Vol. 7, Is. 6. - С. 716-727. - ISSN 20777000 (ISSN) , DOI 10.5943/mycosphere/7/6/2
Ключевые слова (''Своб.индексиров.''): light emitting wood--luminous mycelia
Аннотация: The present study describes changes in the mycelium of the fungus growing on the luminescent wood collected on Borneo Island in early December 2013 that occurred during 31 months of storage. The study shows that wood samples retain their ability to emit light, forming two types of luminescent mycelium: surface mycelium and aerial mycelium. The hyphae of the surface mycelium form on the surface of the wood sample and then spread over the surface of the polyethylene bag or over the surface of the bottom of tissue culture flasks containing the samples. The aerial mycelium develops later and only in tissue culture flasks, forming biomass composed of local interlaced hyphae, growing upward. The surface mycelium is characterized by non-uniform "flickering" luminescence along the hyphae. There is no diurnal periodicity in the luminescence of this fungus, but luminescence is increased by mechanical disturbance or exposure to ultraviolet radiation. The local impact of these factors causes an increase in luminescence of the mycelium regions that have not been directly affected. It has been assumed that the variable level of luminescence is an individual trait of this fungus species. The results obtained in this study suggest that luminescent wood found on Borneo Island contains mycelium of a fungus species, whose luminescent properties are essentially different from those of the fungi described in the scientific literature.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondar V.S., Puzyr' A.P., Purtov K.V., Petunin A.I., Burov A.E., Rodicheva E.K., Medvedeva S.E., Shpak B.A., Tyaglik A.B., Shimomura O..., Gitel'zon I.I.
Заглавие : Isolation of luminescence system from the luminescent fungus Neonothopanus nambi
Колич.характеристики :3 с
Коллективы : Program of the Government of the Russian Federation "On Measures to Attract the Leading Scientists to the Educational Institutions of Russia" [11, G34.31.0058]; Siberian Branch of the Russian Academy of Sciences [71]
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2014. - Vol. 455, Is. 1. - С. 56-58. - ISSN 1607-6729, DOI 10.1134/S1607672914020045. - ISSN 1608-3091
Примечания : Cited References: 10. - This study was supported by the Program of the Government of the Russian Federation "On Measures to Attract the Leading Scientists to the Educational Institutions of Russia" (project no. 11, G34.31.0058) and the Siberian Branch of the Russian Academy of Sciences (project no. 71).
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